Starting /dee2/code/volunteer_pipeline.sh SRR26075346 current disk space = 3050549334016 free memory = 1389654368 SRR26075346 SRAfilesize cb1d7a13451c9d54adc905002bf7e515 SRR26075346.sra SRR26075346.sra file validated SRR26075346 is paired end SRR26075346 is conventional basespace SRR26075346 read1 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR26075346_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.69025 37.0 37.0 37.0 37.0 37.0 2 36.6565 37.0 37.0 37.0 37.0 37.0 3 36.73 37.0 37.0 37.0 37.0 37.0 4 36.7015 37.0 37.0 37.0 37.0 37.0 5 36.7465 37.0 37.0 37.0 37.0 37.0 6 36.7295 37.0 37.0 37.0 37.0 37.0 7 36.725 37.0 37.0 37.0 37.0 37.0 8 36.6435 37.0 37.0 37.0 37.0 37.0 9 36.6565 37.0 37.0 37.0 37.0 37.0 10-14 36.660650000000004 37.0 37.0 37.0 37.0 37.0 15-19 36.5938 37.0 37.0 37.0 37.0 37.0 20-24 36.5693 37.0 37.0 37.0 37.0 37.0 25-29 36.5227 37.0 37.0 37.0 37.0 37.0 30-34 36.3912 37.0 37.0 37.0 37.0 37.0 35-39 36.3448 37.0 37.0 37.0 37.0 37.0 40-44 36.23109999999999 37.0 37.0 37.0 37.0 37.0 45-49 36.268499999999996 37.0 37.0 37.0 37.0 37.0 50-54 36.2121 37.0 37.0 37.0 37.0 37.0 55-59 36.1444 37.0 37.0 37.0 37.0 37.0 60-64 36.0921 37.0 37.0 37.0 37.0 37.0 65-69 36.0817 37.0 37.0 37.0 37.0 37.0 70-74 36.0128 37.0 37.0 37.0 37.0 37.0 75-79 35.9551 37.0 37.0 37.0 37.0 37.0 80-84 35.9192 37.0 37.0 37.0 37.0 37.0 85-89 35.781400000000005 37.0 37.0 37.0 37.0 37.0 90-94 35.8112 37.0 37.0 37.0 37.0 37.0 95-99 35.795300000000005 37.0 37.0 37.0 37.0 37.0 100-104 35.7725 37.0 37.0 37.0 37.0 37.0 105-109 35.677099999999996 37.0 37.0 37.0 37.0 37.0 110-114 35.5745 37.0 37.0 37.0 37.0 37.0 115-119 35.4105 37.0 37.0 37.0 34.6 37.0 120-124 35.51990000000001 37.0 37.0 37.0 37.0 37.0 125-129 35.3129 37.0 37.0 37.0 37.0 37.0 130-134 35.1851 37.0 37.0 37.0 29.8 37.0 135-139 35.014700000000005 37.0 37.0 37.0 27.4 37.0 140-144 34.927600000000005 37.0 37.0 37.0 25.0 37.0 145-149 34.9883 37.0 37.0 37.0 25.0 37.0 150-151 34.872749999999996 37.0 37.0 37.0 25.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.0 1101 2 0.0 1101 3 0.0 1101 4 0.0 1101 5 0.0 1101 6 0.0 1101 7 0.0 1101 8 0.0 1101 9 0.0 1101 10-14 0.0 1101 15-19 0.0 1101 20-24 0.0 1101 25-29 0.0 1101 30-34 0.0 1101 35-39 0.0 1101 40-44 0.0 1101 45-49 0.0 1101 50-54 0.0 1101 55-59 0.0 1101 60-64 0.0 1101 65-69 0.0 1101 70-74 0.0 1101 75-79 0.0 1101 80-84 0.0 1101 85-89 0.0 1101 90-94 0.0 1101 95-99 0.0 1101 100-104 0.0 1101 105-109 0.0 1101 110-114 0.0 1101 115-119 0.0 1101 120-124 0.0 1101 125-129 0.0 1101 130-134 0.0 1101 135-139 0.0 1101 140-144 0.0 1101 145-149 0.0 1101 150-151 0.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 19 1.0 20 4.0 21 2.0 22 3.0 23 4.0 24 9.0 25 8.0 26 9.0 27 20.0 28 24.0 29 28.0 30 37.0 31 37.0 32 51.0 33 106.0 34 164.0 35 475.0 36 2830.0 37 188.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.776609065865266 14.675682444277486 7.062359128474831 34.48534936138242 2 19.8 13.175 35.775 31.25 3 17.424999999999997 15.85 30.65 36.075 4 21.875 22.825 24.925 30.375000000000004 5 22.6 30.099999999999998 25.374999999999996 21.925 6 21.9 30.575000000000003 26.125 21.4 7 16.825000000000003 26.950000000000003 39.25 16.975 8 16.900000000000002 27.775 32.9 22.425 9 17.4 24.575 33.85 24.175 10-14 19.39290893634045 29.76446466970046 27.679151872780917 23.163474521178177 15-19 19.7 27.615000000000002 27.98 24.705 20-24 18.345 27.935 28.985 24.735 25-29 18.915000000000003 28.095 28.725 24.265 30-34 20.18 26.735 28.825 24.26 35-39 20.185 29.409999999999997 26.695 23.71 40-44 19.06 28.754999999999995 27.935 24.25 45-49 20.385 27.16 27.98 24.474999999999998 50-54 19.715 27.595 28.835 23.855 55-59 18.86 27.089999999999996 29.675 24.375 60-64 19.88 26.865 28.939999999999998 24.315 65-69 19.564999999999998 27.700000000000003 27.965 24.77 70-74 19.82 27.045 28.715000000000003 24.42 75-79 19.775000000000002 27.685 27.77 24.77 80-84 19.61 27.52 28.18 24.69 85-89 20.665 26.465 28.544999999999998 24.325 90-94 20.685000000000002 27.125 28.32 23.87 95-99 20.880000000000003 27.450000000000003 27.24 24.43 100-104 19.950000000000003 27.315 27.915 24.82 105-109 20.84 27.49 27.77 23.9 110-114 21.3 27.875 27.765 23.06 115-119 20.8 26.935 28.015 24.25 120-124 20.03 27.495000000000005 27.589999999999996 24.884999999999998 125-129 21.505 27.750000000000004 27.38 23.365 130-134 20.03 27.11 27.834999999999997 25.025 135-139 21.335 26.3 28.27 24.095 140-144 20.599999999999998 27.685 27.425 24.29 145-149 20.805 27.08 27.36 24.755 150-151 21.587500000000002 26.674999999999997 27.675 24.0625 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 0.0 1 0.0 2 0.5 3 1.0 4 1.0 5 1.0 6 0.5 7 1.5 8 2.5 9 1.0 10 0.0 11 0.0 12 0.5 13 0.5 14 0.5 15 0.5 16 0.5 17 0.5 18 0.5 19 1.0 20 3.5 21 3.0 22 3.5 23 5.0 24 3.0 25 2.5 26 4.0 27 7.0 28 8.5 29 14.5 30 18.0 31 18.5 32 23.5 33 29.0 34 43.0 35 62.0 36 70.0 37 86.0 38 115.5 39 153.0 40 187.5 41 204.0 42 215.0 43 261.0 44 281.0 45 266.5 46 258.0 47 255.5 48 267.5 49 245.5 50 177.0 51 120.0 52 117.0 53 118.0 54 98.5 55 67.5 56 42.0 57 34.0 58 28.0 59 14.0 60 11.0 61 8.5 62 8.5 63 13.0 64 5.5 65 2.0 66 3.0 67 2.0 68 1.0 69 1.0 70 0.5 71 0.0 72 0.0 73 0.0 74 0.0 75 0.0 76 0.0 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.17500000000000002 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.015 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150-151 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 60.525 #Duplication Level Percentage of deduplicated Percentage of total 1 62.783973564642714 38.0 2 20.73523337463858 25.1 3 9.830648492358529 17.849999999999998 4 4.171829822387443 10.100000000000001 5 1.3630731102850062 4.125 6 0.49566294919454773 1.7999999999999998 7 0.12391573729863693 0.525 8 0.3304419661296985 1.6 9 0.16522098306484925 0.8999999999999999 >10 0.0 0.0 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTGGTGGAGGATGCTTCCCAGCAACCACAGTTAAAACCCCATTTTCGCAT 9 0.22499999999999998 No Hit ATCAGAACTTGGTTCTCTTCCATTCAGTTGATGCAGAGAACCACCTAGCA 9 0.22499999999999998 No Hit GCCTGATCGATGGAGTTTCTCAAAGAGAGTAGGTTTCTTCTCCTCTTCCT 9 0.22499999999999998 No Hit AGCCGACTTCAGAAACCACTAGCAATTCACTCAATCATCTCGTCGTCGTC 9 0.22499999999999998 No Hit AGTAGCATCAACTCTGTTTTGATCTTGTACAGAGCCTGAATGAGGTACTT 8 0.2 No Hit CTTCCTTCCTCTCCGACGCAGAGGACTGAGGAGTGGACGTCTCCTCCGGA 8 0.2 No Hit GGGGAGTTTTCTTAAACCAATCCTTCGACTAGTTCAGATCATCAGGTGAG 8 0.2 No Hit CTGCAATTATAGTTGCTTAAAATCAAAATTTATGGGATTACTGATGTGTT 8 0.2 No Hit GCCAGGCAGAAGAAGTTGCCATGTCTCTATCTCGCCAGCGAAGGTAAAGA 8 0.2 No Hit GTCACACAAGCTGGCTAAGCACCATCATCTTCCAGGCTGTTGACCCTTTC 8 0.2 No Hit GTGAAATTTGATGTGAGCAATCCAACCTCTCCATCATCCTCTTATTTTTC 8 0.2 No Hit GCTCCTTCCTCCATACCCTTCAACCTCTTCCTCGTAACTCTGCTTCCTGC 8 0.2 No Hit CTGACACAGAATCTTGGGATTCTTTGACTGGTTCACTGACAACTGTAGTG 7 0.17500000000000002 No Hit CATGTAATCAACAAATTGGCTGCTACCTAATGCCACAGATGCAACTTCTT 7 0.17500000000000002 No Hit GCCTTGGCAATAACCTCCTCAATACCGCCAACCATGTAAAATGACTGCTC 7 0.17500000000000002 No Hit TTTTCATCCGGAACTCCGAAGGCGACAGCCTGAGCAATGTCAGGATGAGA 6 0.15 No Hit GCTCCCAGCAAAAAAGCTCCCCGCAACGACATCGGAATCCGGTCAACCCT 6 0.15 No Hit CTGGGAGATCAGCCTTGAAAACGTGAGCCTCGGGGGTCTCCTTCCAGTCT 6 0.15 No Hit GGCAGTTAAACCAACATTAATACCACAACTATCTTAATTGCCACTGACTA 6 0.15 No Hit ACCTCTTGCTGTTCTGGCTCCAATTTGGGTGTCAGCAACTTAATGTACCT 6 0.15 No Hit GGGACAAATCAATAACGCAACAAACACGTGAAGTTAAGGAGAGTAAACAT 6 0.15 No Hit TTTTTTTTATAAAACAAAAATATAATTAAACTCAAAATTAGCCATGCAAA 6 0.15 No Hit GACCAGCTGAAAGATTATCCAATGGTTAAGTTCAGGCAATGTGCCATGTT 6 0.15 No Hit CTGCACCTTCAGCTATCACAATAACCATGTGCCCATTTTCTTTCAGTTGT 6 0.15 No Hit GCGTGGTAGAATTACTGGCACTCCAATGATTCCATATAACGGCCATAATG 6 0.15 No Hit GTAGCTTAGAAACCATGAATTTTAATGCTTTCCTTCTTCACAATGCCAGC 6 0.15 No Hit GTCCGTATCATCACCAGCAGCCTCATCCTCATCAATGCTAAGACCCAGTT 6 0.15 No Hit GTTCTGGATTCATTACTGGCCGTTTCAAAGGAATAGGGTTCCCAAACTGC 5 0.125 No Hit CCTGGTACTCAGCTGCTTCTGACTTGGCCTTTGCAATTCTCTTCTTTTTG 5 0.125 No Hit CTTCTTTTTTGGGTTCCTCCTTGGCCTTTGACTTAGCAGATTCTTTGGGC 5 0.125 No Hit GAAGAAATGAAGGCTCAGTAAGGAAGTATTGTCTCAAAACAGTGAACTCC 5 0.125 No Hit CGGTTCCGTCGCCACTGCCAGCCAAGCGTGCCTCAAGACCATTGGCGAAG 5 0.125 No Hit GTGTGGCAAATCTTTCTCAACTTGCCCACTATGCAAACTGCATCGATGTC 5 0.125 No Hit GGTTCATGAGATTGGTAATTTTGTGGCAAATGTGGTTGAGGTTCTTGCGA 5 0.125 No Hit GGCAGCAGAGGTTCATTATCCTTGGAGAGACTTAGAAGAGATAGTAGTCC 5 0.125 No Hit GCTCAGGCATCTCCAAAGTCTTTGTTTGTGAGTCCAAGGATAACTTCTGC 5 0.125 No Hit GCTCTTGATGCCTCTCTCTTGAGAATCTGCGAGGGCCATCAATATAATTT 5 0.125 No Hit GTCACTCAAAAGTCGATCTAACTTCTCCGCAATAGAAGGTGTAACTCTTA 5 0.125 No Hit ATCCGGGCAGGCATTTATCTGAATAGCATTTTCTTAACTTCATATCCTTG 5 0.125 No Hit CCAGCGCCTTGATACATCTTGGCAATTATAGGATTGCAAATGCTCTCCAG 5 0.125 No Hit GGGTTATCTTTTGGAAAAATTGAATCAAATGAGTCTCGCATGGTTGGATC 5 0.125 No Hit GGAAAATCCATGGACGACAAAACAACACCAGACTGAAGGGCATCAAGGAA 5 0.125 No Hit GGCTATCAAAAGATTTCATCGAATTGACATCATATACAAGAACACAACAA 5 0.125 No Hit TCTCGCTTCGTAGGAAACCAAGACTGAAGTCGTCAGGTGAACCTATGAGC 5 0.125 No Hit GTGCGAAGAGAATTGGATGCCAAAACACCAGAGGGTGCAGATTGTGCAGT 5 0.125 No Hit GGAAGCTATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTG 5 0.125 No Hit CTTAAATATAATGCAAATATCAAATTTTGTAATGATTGAGCAATTTAAGA 5 0.125 No Hit GATGCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTA 5 0.125 No Hit GTCAGGGTACATCTTGCATCCTCCACAGCCGCTGCCGCACTTGCAGCCAG 5 0.125 No Hit GCTGGGATGTTTGAGTGCCTCTTCTCATATCTCTGGTATTTTTTGACCCA 5 0.125 No Hit CTTCTACTTTTATTTAATAGTTTTATAGATTACACAAAGGAAATACAACA 5 0.125 No Hit GTGACATTGGATTGCCGGTTGGTTCGAATTTCATGGTCATGAAGAAGTGA 5 0.125 No Hit GCTCTGGGTAACCCAAAAGCATATAAGCCATTGCCAATTGCGAAAGCAAT 5 0.125 No Hit CAAGCGAAAAACACTTTGAAAAACAATTACTACCACGATACCAAACAGGC 5 0.125 No Hit CTCTGAGTATGAGCTGGTGGAGGAGCATGGTTAACTGTTTGGGGAGGTGG 5 0.125 No Hit GCAACATCAATCTCCGAAGTGACGATTTTCAAGGATGGGAATCTTTTGCA 5 0.125 No Hit GATCATTTCCTTTGTTCATGGTAGCGTGTCCCTCTGACTTACAATTCATG 5 0.125 No Hit GTACATTGATCCACCAACATTGGGTATCCCAGACATTGATTCAAACTCTC 5 0.125 No Hit CCGGCAGGTTCAAAGGGGGCAAGGTTGAAGCCGGCAGGTTCAAAGGGGGC 5 0.125 No Hit GGTCGTAGATCATGGCGGTTGGTGGTGGTGGGGTTGGTGAAGTCAAGGGA 5 0.125 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0 0.0 0.0 0.0 0.0 64-65 0.0125 0.0 0.0 0.0 0.0 66-67 0.025 0.0 0.0 0.0 0.0 68-69 0.05 0.0 0.0 0.0 0.0 70-71 0.05 0.0 0.0 0.0 0.0 72-73 0.05 0.0 0.0 0.0 0.0 74-75 0.05 0.0 0.0 0.0 0.0 76-77 0.05 0.0 0.0 0.0 0.0 78-79 0.05 0.0 0.0 0.0 0.0 80-81 0.05 0.0 0.0 0.0 0.0 82-83 0.075 0.0 0.0 0.0 0.0 84-85 0.0875 0.0 0.0 0.0 0.0 86-87 0.1375 0.0 0.0 0.0 0.0 88-89 0.16249999999999998 0.0 0.0 0.0 0.0 90-91 0.23750000000000002 0.0 0.0 0.0 0.0 92-93 0.4 0.0 0.0 0.0 0.0 94-95 0.44999999999999996 0.0 0.0 0.0 0.0 96-97 0.5 0.0 0.0 0.0 0.0 98-99 0.65 0.0 0.0 0.0 0.0 100-101 1.0875 0.0 0.0 0.0 0.0 102-103 1.4125 0.0 0.0 0.0 0.0 104-105 1.6375000000000002 0.0 0.0 0.0 0.0 106-107 1.85 0.0 0.0 0.0 0.0 108-109 2.0625 0.0 0.0 0.0 0.0 110-111 2.25 0.0 0.0 0.0 0.0 112-113 2.4375 0.0 0.0 0.0 0.0 114-115 2.8875 0.0 0.0 0.0 0.0 116-117 3.2375 0.0 0.0 0.0 0.0 118-119 3.75 0.0 0.0 0.0 0.0 120-121 4.0625 0.0 0.0 0.0 0.0 122-123 4.475 0.0 0.0 0.0 0.0 124-125 5.0375 0.0 0.0 0.0 0.0 126-127 5.4625 0.0 0.0 0.0 0.0 128-129 5.875 0.0 0.0 0.0 0.0 130-131 6.425 0.0 0.0 0.0 0.0 132-133 6.612500000000001 0.0 0.0 0.0 0.0 134-135 7.3625 0.0 0.0 0.0 0.0 136-137 8.225 0.0 0.0 0.0 0.0 138-139 8.850000000000001 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GCACCTT 10 0.006830828 145.0 1 >>END_MODULE SRR26075346 read2 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR26075346_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 35.8385 37.0 37.0 37.0 37.0 37.0 2 36.0515 37.0 37.0 37.0 37.0 37.0 3 36.07 37.0 37.0 37.0 37.0 37.0 4 36.052 37.0 37.0 37.0 37.0 37.0 5 36.152 37.0 37.0 37.0 37.0 37.0 6 36.03 37.0 37.0 37.0 37.0 37.0 7 36.1125 37.0 37.0 37.0 37.0 37.0 8 36.1385 37.0 37.0 37.0 37.0 37.0 9 36.202 37.0 37.0 37.0 37.0 37.0 10-14 36.1305 37.0 37.0 37.0 37.0 37.0 15-19 36.07809999999999 37.0 37.0 37.0 37.0 37.0 20-24 36.0558 37.0 37.0 37.0 37.0 37.0 25-29 35.9322 37.0 37.0 37.0 37.0 37.0 30-34 35.8197 37.0 37.0 37.0 37.0 37.0 35-39 35.7506 37.0 37.0 37.0 37.0 37.0 40-44 35.784499999999994 37.0 37.0 37.0 37.0 37.0 45-49 35.7477 37.0 37.0 37.0 37.0 37.0 50-54 35.6376 37.0 37.0 37.0 37.0 37.0 55-59 35.7483 37.0 37.0 37.0 37.0 37.0 60-64 35.674600000000005 37.0 37.0 37.0 37.0 37.0 65-69 35.6531 37.0 37.0 37.0 37.0 37.0 70-74 35.603500000000004 37.0 37.0 37.0 37.0 37.0 75-79 35.4425 37.0 37.0 37.0 37.0 37.0 80-84 35.5683 37.0 37.0 37.0 37.0 37.0 85-89 35.5159 37.0 37.0 37.0 37.0 37.0 90-94 35.358 37.0 37.0 37.0 34.6 37.0 95-99 35.4414 37.0 37.0 37.0 37.0 37.0 100-104 35.3556 37.0 37.0 37.0 34.6 37.0 105-109 35.2736 37.0 37.0 37.0 32.2 37.0 110-114 35.1664 37.0 37.0 37.0 29.8 37.0 115-119 35.151900000000005 37.0 37.0 37.0 29.8 37.0 120-124 35.0558 37.0 37.0 37.0 27.4 37.0 125-129 34.971900000000005 37.0 37.0 37.0 25.0 37.0 130-134 34.9196 37.0 37.0 37.0 25.0 37.0 135-139 34.7735 37.0 37.0 37.0 25.0 37.0 140-144 34.808550000000004 37.0 37.0 37.0 25.0 37.0 145-149 34.816250000000004 37.0 37.0 37.0 25.0 37.0 150-151 34.383875 37.0 37.0 37.0 25.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.0 1101 2 0.0 1101 3 0.0 1101 4 0.0 1101 5 0.0 1101 6 0.0 1101 7 0.0 1101 8 0.0 1101 9 0.0 1101 10-14 0.0 1101 15-19 0.0 1101 20-24 0.0 1101 25-29 0.0 1101 30-34 0.0 1101 35-39 0.0 1101 40-44 0.0 1101 45-49 0.0 1101 50-54 0.0 1101 55-59 0.0 1101 60-64 0.0 1101 65-69 0.0 1101 70-74 0.0 1101 75-79 0.0 1101 80-84 0.0 1101 85-89 0.0 1101 90-94 0.0 1101 95-99 0.0 1101 100-104 0.0 1101 105-109 0.0 1101 110-114 0.0 1101 115-119 0.0 1101 120-124 0.0 1101 125-129 0.0 1101 130-134 0.0 1101 135-139 0.0 1101 140-144 0.0 1101 145-149 0.0 1101 150-151 0.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 13 3.0 14 2.0 15 7.0 16 6.0 17 3.0 18 6.0 19 5.0 20 2.0 21 7.0 22 9.0 23 12.0 24 13.0 25 10.0 26 10.0 27 8.0 28 10.0 29 18.0 30 31.0 31 43.0 32 57.0 33 144.0 34 278.0 35 917.0 36 2269.0 37 130.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.375 22.425 11.225 20.974999999999998 2 31.6 26.875 23.775 17.75 3 24.0 29.875 28.799999999999997 17.325 4 25.35 33.825 22.375 18.45 5 25.124999999999996 38.2 21.3 15.375 6 22.875 38.25 21.7 17.175 7 21.25 24.375 36.449999999999996 17.925 8 23.375 26.900000000000002 26.174999999999997 23.549999999999997 9 23.5 25.7 27.875 22.925 10-14 24.92 30.349999999999998 24.18 20.549999999999997 15-19 24.62 28.29 26.75 20.34 20-24 24.36 29.054999999999996 26.22 20.365 25-29 24.255 28.64 25.729999999999997 21.375 30-34 25.224999999999998 27.88 26.6 20.294999999999998 35-39 24.785 28.694999999999997 26.119999999999997 20.4 40-44 24.525 28.52 26.8 20.155 45-49 24.610000000000003 28.71 26.705000000000002 19.975 50-54 24.465 27.985 26.985 20.565 55-59 24.25 29.020000000000003 26.174999999999997 20.555 60-64 24.69 28.845 25.919999999999998 20.544999999999998 65-69 24.279999999999998 29.26 26.284999999999997 20.175 70-74 24.404999999999998 29.685 25.935000000000002 19.975 75-79 23.400000000000002 29.29 26.295 21.015 80-84 24.715 30.125 24.779999999999998 20.380000000000003 85-89 24.69 28.83 27.005000000000003 19.475 90-94 24.01 28.515 27.400000000000002 20.075000000000003 95-99 25.074999999999996 29.385 25.31 20.23 100-104 24.935 28.845 26.05 20.169999999999998 105-109 25.195 28.87 25.395 20.54 110-114 24.68 28.744999999999997 26.474999999999998 20.1 115-119 25.224999999999998 28.785 25.86 20.13 120-124 25.85 28.4 26.369999999999997 19.38 125-129 25.88 29.145 25.915 19.06 130-134 26.185000000000002 28.549999999999997 26.584999999999997 18.68 135-139 26.0 28.59 26.22 19.189999999999998 140-144 25.51127556377819 28.63643182159108 26.811340567028353 19.04095204760238 145-149 27.2590888633295 28.479271890783618 25.11376706505976 19.147872180827125 150-151 25.065633204150515 28.491061382672832 26.453306663332913 19.98999874984373 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.5 6 0.5 7 0.5 8 1.5 9 1.5 10 0.5 11 0.5 12 1.5 13 2.0 14 1.0 15 0.0 16 1.0 17 1.5 18 1.5 19 1.0 20 1.5 21 1.5 22 1.5 23 3.5 24 4.0 25 4.0 26 4.5 27 3.5 28 3.0 29 7.5 30 10.5 31 11.5 32 15.0 33 15.5 34 13.5 35 44.0 36 78.5 37 93.5 38 108.5 39 136.0 40 160.0 41 204.5 42 289.0 43 314.0 44 290.0 45 286.0 46 276.5 47 282.5 48 254.0 49 206.0 50 169.0 51 129.5 52 123.0 53 105.5 54 83.0 55 62.0 56 45.0 57 29.0 58 20.5 59 13.0 60 7.5 61 9.0 62 6.0 63 3.5 64 3.5 65 5.0 66 7.0 67 4.5 68 1.0 69 2.0 70 1.0 71 0.0 72 0.0 73 0.5 74 1.5 75 2.0 76 1.0 77 0.5 78 0.5 79 0.0 80 0.0 81 0.5 82 2.0 83 1.5 84 0.0 85 0.5 86 1.5 87 1.5 88 1.0 89 0.5 90 0.0 91 0.0 92 0.5 93 0.5 94 0.0 95 0.0 96 0.0 97 0.5 98 2.0 99 2.0 100 6.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.005 145-149 0.015 150-151 0.0125 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 61.375 #Duplication Level Percentage of deduplicated Percentage of total 1 64.4806517311609 39.574999999999996 2 19.918533604887983 24.45 3 9.327902240325866 17.175 4 3.8696537678207736 9.5 5 1.1405295315682282 3.5000000000000004 6 0.4887983706720978 1.7999999999999998 7 0.20366598778004072 0.8750000000000001 8 0.40733197556008144 2.0 9 0.12219959266802445 0.675 >10 0.04073319755600815 0.44999999999999996 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG 18 0.44999999999999996 No Hit GATCAATTTTATTAATTACAGGTTACTGTTGAAGATGAGAATACTCTGGT 9 0.22499999999999998 No Hit GCCCAGATGGACAGGACTTGGTTTATCGTGTGGAGAAGAATGGCGGGCTC 9 0.22499999999999998 No Hit ATGAGTATGAGACCAAAGTTGGTGAAGAGAGTGGTGCTGTTGAGACCAAG 9 0.22499999999999998 No Hit GGCGAAGTTAACAGCGGATCCGTCGACGAGAATGTATTTGCAGCAGCCGG 8 0.2 No Hit AAGTTGAAAGCGATGGAACGATACCTTCGCCACAATATCATCTGCTTCAT 8 0.2 No Hit GACATTCCTTGCTAAAGCTCTCAACAACCCATCCTTTATCGAGCAATTTG 8 0.2 No Hit TGGATTCTTGGGGTTTAGGAATTCCACCAAATCATTTGTGCACTGGATTG 8 0.2 No Hit GTTAGCTATGAAGGAGCCAACATGAGGAGGCCAGGGTCCGAATATGAAGA 8 0.2 No Hit GCTTAATGTAGCTGATATTGAGGCCACTCTTTCCCGTGTTTGTCAGATGG 8 0.2 No Hit GCTGAGAGGGTTGAACGGCATGAACTTCTTGCGAAGTGGAGGTCTCGATT 8 0.2 No Hit TGGAAAACGAATCCTCTAGTGTTATTTATTTCTCCTTGTACAGAACATAG 8 0.2 No Hit GGCAAACCTCTTTTAGATAAGAATGGCAACCTTCAGATACTCACTTCCCA 8 0.2 No Hit GAGAAAGAGGTTGTGTGTTCATGAAGCAGGAAACAAACCAAGTGGTACTG 8 0.2 No Hit CAGCAATTCACAACGACAATTCTCAGCGTTCAACCAATCATATCAACACC 7 0.17500000000000002 No Hit GTGTTGAATGTCGCATTGATATGAAGAACTTGATTGGTGAGGCTGTGTGT 7 0.17500000000000002 No Hit GGGCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATAGGAGGTTT 7 0.17500000000000002 No Hit CCTGATCCTCTGTGCACAAGCTGTCTCTGCCAATGATTTCAGGACTGCTA 7 0.17500000000000002 No Hit TGAAAAATCGTTTGAGGGAGAGAAAACAGTTGAAAGTGTTGAACCAGCTT 7 0.17500000000000002 No Hit CAAAGCTAGAAATTGTGAAAGGGGATAGTTTTAGGTGGCGTGGTAGCTGT 6 0.15 No Hit GAGAGGGTGAGAGGAGAGCTTTCTCATTCTTCTTTTTACCCTCCAAATTG 6 0.15 No Hit GAACAAGGAACCAGTCAGGTGATTAACAGCAACCCATCAAGCTACAAGAT 6 0.15 No Hit ATGGATTTTTGCACCTTGTCGGTCGGATCAAGGAGCTTATTAACCGTGGA 6 0.15 No Hit GCACTACTGTGGTAAGTGTGGGCTTACCTATGTTTACCAGACGGCTGGTG 6 0.15 No Hit AGGAATGGGAGTATTTGCACTTGTGGTAACGGTATTTGCATTATTGATGG 6 0.15 No Hit GCAATGGTAGGAAAAGCTTGACAACTGTGCAAGGTTTGAAAAAAGAATTC 6 0.15 No Hit CCCATACAAGGAAATTGAGAATGGAGTGCTTTGGGAAGTGGAAGGAAAGT 6 0.15 No Hit CAGAAAGCAGTGCACTGTTGCAAACAAGCACCACTAGAAACTTTGATTTG 6 0.15 No Hit GTGGGTTTATTGCTATGTATGCTACTCTTGCAAGCCGAGATGTGGATTGT 6 0.15 No Hit GATCATCAAAGGCAACCAAAAACTGCCTTGGATAAAGAAAGATCTGACAG 6 0.15 No Hit CGAAGAAGTAACATCTTTGATCCTTTCTCTCTTGACATCTGGGACCCTTT 6 0.15 No Hit AGACAATGGAAAACAGCTTATATATGAGAAGCTCCCCAAGGATATTTCAG 5 0.125 No Hit GTTAGGGGCTCCATCGCGTATATGATCAACACATGCGATGATCTTGAACG 5 0.125 No Hit CATTCACGGAGTCATCTATTTTGGGAATGCTGGAAGCTTGGATAAGAAAA 5 0.125 No Hit GAGTAATAGAAGGGGTCATCGATCTCGACCAATAAGCATCACATGTTTGT 5 0.125 No Hit GTAGTCTATACTCGTGGAGTTTGGAGGATTAAGGGCATTATACAGGTATC 5 0.125 No Hit GAATCTGATCCATCACCTCAGTTCCACGATCAAGTTAATGATGAGCCCTA 5 0.125 No Hit GTACATTCGGCAAATGTCTTGCATCGTGATCTAAAACCCAGTAATTTGTT 5 0.125 No Hit GGTGTCATCACAGCGCAATCCCACCAATATGCCTGCTATCCAGCATCTTG 5 0.125 No Hit GCGAGAAGAGAAAGATGGATTTCAGAATCATGGGTCTTGACGCGCCACTC 5 0.125 No Hit ATTCAACAAACCCTAAACAGCCAAACCAAAGATCATGGCTTGGAAGCGCC 5 0.125 No Hit CAAGACCTCTCTGTTCTGAACTTGGTTATTGTGAAGAAGGGTGATAATGA 5 0.125 No Hit ACAGTACTCGATGATTCATCGTCTTGAAACAAATAAGCTGCGCAATGTGG 5 0.125 No Hit AAACCCTAAAAGCTCAAACCTTTGGCGGCTATCCACTGAACCCACCGAAG 5 0.125 No Hit GAACTCTTCCGCACTATCACACAATATCAGACTTGCAGAGAAGCTTTGGA 5 0.125 No Hit CTCTGCGGAAACAGAGACGTTTGAGCAGGAGTAGGCTGATCATTCCCATC 5 0.125 No Hit CTCTAGAAGCCTCTCTCTGTCTATCTAATCGCCGCCTATCTCATCATCCA 5 0.125 No Hit GAGGTATCCCAATAGGAGGTTTCCTCCTATGGTTTTCAAAACAATCACCA 5 0.125 No Hit GCAAGGAAACCAGAATTTTTCCCCTCCATTCAAGTAATCAGAGAAAAGGT 5 0.125 No Hit CAAACATCTAATTCTACAATAAGCTAGCTTTTACGTTGCAAACCTTGTTT 5 0.125 No Hit AGCAGCAGACATTTCTCAACAACCACCGCCATGGCTGAGCAGACGGAGAA 5 0.125 No Hit CATCAACTTTGCCTTCAACTGTACCCCTAATGCCCTCTGCCTCTCTGCCT 5 0.125 No Hit ACCATGCTGTGGCGATGATGCTGTTCAGGATGTAGCAAGCAAGAACTCGC 5 0.125 No Hit GAACTATTCAGATCTTGTTGCTTATGGCCGTGTGTTCTTGGCCAATCCAG 5 0.125 No Hit CGAGCTTCTAATTCATCATCTCCTGGCAAGACAAGATCTATATCAGGCAG 5 0.125 No Hit CTTTGAGGCTTGTGTCAAAGCGGTTTGGATTGGGGATCTCTACTTGTCAT 5 0.125 No Hit ATGAGAAGATTGGATCCCAACTTAGCCCAGCTGATAAGAAGAAAATCGAG 5 0.125 No Hit GCTACTATGGCCCCCCTAATGGGATTCCACATTTACACCAATCTTTGCTT 5 0.125 No Hit GGAGCCTTTTGGTATTTGTTCTCAATAGAACGAGAAACAAATTGTTGGAG 5 0.125 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0 0.0 0.0 0.0 0.0 64-65 0.0 0.0 0.0 0.0 0.0 66-67 0.0 0.0 0.0 0.0 0.0 68-69 0.025 0.0 0.0 0.0 0.0 70-71 0.025 0.0 0.0 0.0 0.0 72-73 0.025 0.0 0.0 0.0 0.0 74-75 0.025 0.0 0.0 0.0 0.0 76-77 0.025 0.0 0.0 0.0 0.0 78-79 0.025 0.0 0.0 0.0 0.0 80-81 0.025 0.0 0.0 0.0 0.0 82-83 0.05 0.0 0.0 0.0 0.0 84-85 0.0625 0.0 0.0 0.0 0.0 86-87 0.1125 0.0 0.0 0.0 0.0 88-89 0.1375 0.0 0.0 0.0 0.0 90-91 0.2125 0.0 0.0 0.0 0.0 92-93 0.375 0.0 0.0 0.0 0.0 94-95 0.42500000000000004 0.0 0.0 0.0 0.0 96-97 0.475 0.0 0.0 0.0 0.0 98-99 0.6375 0.0 0.0 0.0 0.0 100-101 1.1125 0.0 0.0 0.0 0.0 102-103 1.4625 0.0 0.0 0.0 0.0 104-105 1.6875 0.0 0.0 0.0 0.0 106-107 1.9 0.0 0.0 0.0 0.0 108-109 2.1125 0.0 0.0 0.0 0.0 110-111 2.2875 0.0 0.0 0.0 0.0 112-113 2.4749999999999996 0.0 0.0 0.0 0.0 114-115 2.9375 0.0 0.0 0.0 0.0 116-117 3.3375 0.0 0.0 0.0 0.0 118-119 3.8625 0.0 0.0 0.0 0.0 120-121 4.1875 0.0 0.0 0.0 0.0 122-123 4.625 0.0 0.0 0.0 0.0 124-125 5.1875 0.0 0.0 0.0 0.0 126-127 5.6125 0.0 0.0 0.0 0.0 128-129 6.025 0.0 0.0 0.0 0.0 130-131 6.575 0.0 0.0 0.0 0.0 132-133 6.775 0.0 0.0 0.0 0.0 134-135 7.550000000000001 0.0 0.0 0.0 0.0 136-137 8.45 0.0 0.0 0.0 0.0 138-139 9.075 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTTAGAG 10 0.006830828 145.0 5 CCTTAGA 10 0.006830828 145.0 4 >>END_MODULE Read 1596639 spots for SRR26075346.sra Written 1596639 spots for SRR26075346.sra Read 1596639 spots for SRR26075346.sra Written 1596639 spots for SRR26075346.sra Read 1596639 spots for SRR26075346.sra Written 1596639 spots for SRR26075346.sra Read 1596639 spots for SRR26075346.sra Written 1596639 spots for SRR26075346.sra Read 1596639 spots for SRR26075346.sra Written 1596639 spots for SRR26075346.sra Read 1596639 spots for SRR26075346.sra Written 1596639 spots for SRR26075346.sra Read 1596639 spots for SRR26075346.sra Written 1596639 spots for SRR26075346.sra Read 1596639 spots for SRR26075346.sra Written 1596639 spots for SRR26075346.sra Read 1596639 spots for SRR26075346.sra Written 1596639 spots for SRR26075346.sra Read 1596641 spots for SRR26075346.sra Written 1596641 spots for SRR26075346.sra Read 1596639 spots for SRR26075346.sra Written 1596639 spots for SRR26075346.sra Read 1596639 spots for SRR26075346.sra Written 1596639 spots for SRR26075346.sra Read 1596639 spots for SRR26075346.sra Written 1596639 spots for SRR26075346.sra Read 1596639 spots for SRR26075346.sra Written 1596639 spots for SRR26075346.sra Read 1596639 spots for SRR26075346.sra Written 1596639 spots for SRR26075346.sra Read 1596639 spots for SRR26075346.sra Written 1596639 spots for SRR26075346.sra Read 1596639 spots for SRR26075346.sra Written 1596639 spots for SRR26075346.sra Read 1596639 spots for SRR26075346.sra Written 1596639 spots for SRR26075346.sra Read 1596639 spots for SRR26075346.sra Written 1596639 spots for SRR26075346.sra Read 1596639 spots for SRR26075346.sra Written 1596639 spots for SRR26075346.sra SRR ids: ['SRR26075346.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd__d_6cdjl SRR26075346.sra spots: 31932782 blocks: [[1, 1596639], [1596640, 3193278], [3193279, 4789917], [4789918, 6386556], [6386557, 7983195], [7983196, 9579834], [9579835, 11176473], [11176474, 12773112], [12773113, 14369751], [14369752, 15966390], [15966391, 17563029], [17563030, 19159668], [19159669, 20756307], [20756308, 22352946], [22352947, 23949585], [23949586, 25546224], [25546225, 27142863], [27142864, 28739502], [28739503, 30336141], [30336142, 31932782]] SRR26075346 file size 11791342 SRR26075346 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o SRR26075346 SRR26075346_1.fastq SRR26075346_2.fastq Input file: SRR26075346_1.fastq Paired file: SRR26075346_2.fastq trimmed: SRR26075346-trimmed-pair1.fastq, SRR26075346-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Wed Feb 12 02:14:18 2025 >> started Wed Feb 12 02:14:54 2025 >> done (36.287s) 31932782 read pairs processed; of these: 169 ( 0.00%) short read pairs filtered out after trimming by size control 85163 ( 0.27%) empty read pairs filtered out after trimming by size control 31847450 (99.73%) read pairs available; of these: 4356145 (13.68%) trimmed read pairs available after processing 27491305 (86.32%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 18 24 0.00% 19 14 0.00% 20 24 0.00% 21 27 0.00% 22 43 0.00% 23 28 0.00% 24 32 0.00% 25 30 0.00% 26 31 0.00% 27 41 0.00% 28 50 0.00% 29 41 0.00% 30 58 0.00% 31 42 0.00% 32 57 0.00% 33 62 0.00% 34 49 0.00% 35 65 0.00% 36 67 0.00% 37 55 0.00% 38 79 0.00% 39 62 0.00% 40 86 0.00% 41 121 0.00% 42 85 0.00% 43 96 0.00% 44 125 0.00% 45 105 0.00% 46 126 0.00% 47 129 0.00% 48 132 0.00% 49 143 0.00% 50 173 0.00% 51 213 0.00% 52 247 0.00% 53 255 0.00% 54 210 0.00% 55 276 0.00% 56 271 0.00% 57 325 0.00% 58 392 0.00% 59 410 0.00% 60 556 0.00% 61 528 0.00% 62 694 0.00% 63 717 0.00% 64 804 0.00% 65 831 0.00% 66 977 0.00% 67 1068 0.00% 68 1228 0.00% 69 1399 0.00% 70 1618 0.01% 71 1809 0.01% 72 2179 0.01% 73 2419 0.01% 74 2962 0.01% 75 3151 0.01% 76 3588 0.01% 77 3804 0.01% 78 4420 0.01% 79 4823 0.02% 80 5442 0.02% 81 6271 0.02% 82 7165 0.02% 83 7611 0.02% 84 8896 0.03% 85 9527 0.03% 86 10350 0.03% 87 11557 0.04% 88 12183 0.04% 89 13269 0.04% 90 14449 0.05% 91 16210 0.05% 92 17207 0.05% 93 18805 0.06% 94 21584 0.07% 95 22093 0.07% 96 24186 0.08% 97 25456 0.08% 98 25981 0.08% 99 27992 0.09% 100 29602 0.09% 101 30790 0.10% 102 32238 0.10% 103 34492 0.11% 104 36858 0.12% 105 39562 0.12% 106 41204 0.13% 107 43496 0.14% 108 44780 0.14% 109 46722 0.15% 110 46222 0.15% 111 49282 0.15% 112 52177 0.16% 113 52949 0.17% 114 56559 0.18% 115 59348 0.19% 116 60958 0.19% 117 63078 0.20% 118 65245 0.20% 119 66355 0.21% 120 66622 0.21% 121 68725 0.22% 122 71202 0.22% 123 73963 0.23% 124 76708 0.24% 125 77991 0.24% 126 79983 0.25% 127 83739 0.26% 128 84719 0.27% 129 87472 0.27% 130 89472 0.28% 131 88847 0.28% 132 92370 0.29% 133 93695 0.29% 134 96096 0.30% 135 99943 0.31% 136 101035 0.32% 137 102092 0.32% 138 104966 0.33% 139 108021 0.34% 140 107092 0.34% 141 109376 0.34% 142 113388 0.36% 143 113697 0.36% 144 115073 0.36% 145 120913 0.38% 146 121005 0.38% 147 123606 0.39% 148 125060 0.39% 149 127809 0.40% 150 128838 0.40% 151 27491305 86.32% 31847450 reads passed initial QC criterion=sequence-density sequence-density=0.91 sequence-density-rank=1 fanout-score=3.99 fanout-score-rank=17 prefix-density=0.98 prefix-fanout=3.7 sequence=GCATTCTCAGGCAGCCTAAACCTCCTC criterion=fanout-score sequence-density=0.01 sequence-density-rank=38 fanout-score=32.63 fanout-score-rank=1 prefix-density=0.08 prefix-fanout=5.5 sequence=CTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGAGATGTTGGAACTCTACCAATTGGAGCTTTCTTAGCTGTCTTAGCAGTAGTTTATAAGGAATATATCCCATTTTTAGTTATAATGATGCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAACCAATGAGAGAGCCTCACTTAGTTACAGTTTTATGGATAATTGGGATATTCTTTGGTATAGTTGGGATTTTAATATCATTAATAGCATGATGGTGATTGTTTTGAAAACCATAGGAGGAAACCTCC criterion=sequence-density sequence-density=0.49 sequence-density-rank=1 fanout-score=3.76 fanout-score-rank=29 prefix-density=0.76 prefix-fanout=2.4 sequence=AAGATGGCAATGATCCCAAGCTTCTTCGACAACAGACGAGGCACCATCTTTGATCCATTCACTTGGGAACCCTTCAAGGACTT criterion=fanout-score sequence-density=0.03 sequence-density-rank=38 fanout-score=241.08 fanout-score-rank=1 prefix-density=0.45 prefix-fanout=17.6 sequence=AGAGAAGAAACACTAAAAAAATCACACAAGCGAAATCTCCAGCGAGAAGAGAAAGATGGATTTCAGAATCATGGGTCTTGACGCGCCACTCTTCAACACCCTCCAGCACATGATGGATGCAAGTGATCATGAGGCAGACAAGTCCTTCAATGCGCCAACACGCACTTACGTACGTGATGCCAAGGCAATGGCATCAACACCAGCTGATGTGAAAGAGTATCCAA SRR26075346 testing PE reads STAR mapping to Ensembl genome Started job on | Feb 12 02:15:40 Started mapping on | Feb 12 02:15:41 Finished on | Feb 12 02:21:11 Mapping speed, Million of reads per hour | 347.43 Number of input reads | 31847450 Average input read length | 295 UNIQUE READS: Uniquely mapped reads number | 28147955 Uniquely mapped reads % | 88.38% Average mapped length | 293.78 Number of splices: Total | 21119595 Number of splices: Annotated (sjdb) | 20468804 Number of splices: GT/AG | 20767026 Number of splices: GC/AG | 252972 Number of splices: AT/AC | 30933 Number of splices: Non-canonical | 68664 Mismatch rate per base, % | 0.44% Deletion rate per base | 0.03% Deletion average length | 2.85 Insertion rate per base | 0.02% Insertion average length | 2.91 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 1074902 % of reads mapped to multiple loci | 3.38% Number of reads mapped to too many loci | 81875 % of reads mapped to too many loci | 0.26% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 7.69% % of reads unmapped: other | 0.30% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 2624593 2624593 2624593 N_multimapping 1074902 1074902 1074902 N_noFeature 685730 27828066 890095 N_ambiguous 301509 2292 184277 UnstrandedReadsAssigned:27160716 PositiveStrandReadsAssigned:317597 NegativeStrandReadsAssigned:27073583 Dataset is classified negative stranded MeadianReadLen=151 20thPercentileLength=151 echo kmer=147 SRR26075346 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,400 [index] number of k-mers: 62,057,036 [index] number of equivalence classes: 130,681 [quant] running in paired-end mode [quant] will process pair 1: SRR26075346-trimmed-pair1.fastq SRR26075346-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 31,847,450 reads, 27,729,230 reads pseudoaligned [quant] estimated average fragment length: 219.452 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,173 rounds 52401 SRR26075346.ke.tsv 34699 SRR26075346.se.tsv 87100 total ==> SRR26075346.ke.tsv <== target_id length eff_length est_counts tpm Potri.005G200100.1.v4.1 2018 1799.55 5942 94.8073 Potri.005G024800.1.v4.1 1035 816.548 10947 384.933 Potri.004G059700.1.v4.1 961 742.558 31 1.19868 Potri.007G009000.2.v4.1 1416 1197.55 0 0 Potri.003G141000.2.v4.1 2943 2724.55 1122.3 11.8273 Potri.016G087400.1.v4.1 270 86.4201 2903 964.506 Potri.015G069301.1.v4.1 564 347.508 0 0 Potri.010G195200.1.v4.1 1773 1554.55 72 1.32984 Potri.012G127500.1.v4.1 977 758.558 4821 182.482 ==> SRR26075346.se.tsv <== Potri.001G166300.v4.1 0 Potri.001G448400.v4.1 70 Potri.001G233950.v4.1 0 Potri.001G122700.v4.1 167 Potri.001G212900.v4.1 0 Potri.001G182400.v4.1 0 Potri.001G256600.v4.1 0 Potri.001G040500.v4.1 6 Potri.001G416900.v4.1 0 Potri.001G452600.v4.1 432 SRR26075346 completed mapping pipeline successfully