Starting /dee2/code/volunteer_pipeline.sh SRR26075347 current disk space = 3049201455104 free memory = 1429511756 SRR26075347 SRAfilesize 3ae7f13a048e9251a017748690bb8fb2 SRR26075347.sra SRR26075347.sra file validated SRR26075347 is paired end SRR26075347 is conventional basespace SRR26075347 read1 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR26075347_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.62925 37.0 37.0 37.0 37.0 37.0 2 36.6025 37.0 37.0 37.0 37.0 37.0 3 36.5885 37.0 37.0 37.0 37.0 37.0 4 36.6675 37.0 37.0 37.0 37.0 37.0 5 36.6435 37.0 37.0 37.0 37.0 37.0 6 36.656 37.0 37.0 37.0 37.0 37.0 7 36.5725 37.0 37.0 37.0 37.0 37.0 8 36.652 37.0 37.0 37.0 37.0 37.0 9 36.594 37.0 37.0 37.0 37.0 37.0 10-14 36.6411 37.0 37.0 37.0 37.0 37.0 15-19 36.606700000000004 37.0 37.0 37.0 37.0 37.0 20-24 36.605000000000004 37.0 37.0 37.0 37.0 37.0 25-29 36.5769 37.0 37.0 37.0 37.0 37.0 30-34 36.48780000000001 37.0 37.0 37.0 37.0 37.0 35-39 36.4433 37.0 37.0 37.0 37.0 37.0 40-44 36.3626 37.0 37.0 37.0 37.0 37.0 45-49 36.304 37.0 37.0 37.0 37.0 37.0 50-54 36.2034 37.0 37.0 37.0 37.0 37.0 55-59 36.179100000000005 37.0 37.0 37.0 37.0 37.0 60-64 36.150400000000005 37.0 37.0 37.0 37.0 37.0 65-69 36.0167 37.0 37.0 37.0 37.0 37.0 70-74 36.0517 37.0 37.0 37.0 37.0 37.0 75-79 36.025999999999996 37.0 37.0 37.0 37.0 37.0 80-84 36.0251 37.0 37.0 37.0 37.0 37.0 85-89 35.9851 37.0 37.0 37.0 37.0 37.0 90-94 35.9182 37.0 37.0 37.0 37.0 37.0 95-99 35.939499999999995 37.0 37.0 37.0 37.0 37.0 100-104 35.8711 37.0 37.0 37.0 37.0 37.0 105-109 35.7265 37.0 37.0 37.0 37.0 37.0 110-114 35.576499999999996 37.0 37.0 37.0 34.6 37.0 115-119 35.53830000000001 37.0 37.0 37.0 37.0 37.0 120-124 35.5684 37.0 37.0 37.0 37.0 37.0 125-129 35.416 37.0 37.0 37.0 37.0 37.0 130-134 35.2827 37.0 37.0 37.0 34.6 37.0 135-139 35.0978 37.0 37.0 37.0 29.8 37.0 140-144 34.9789 37.0 37.0 37.0 27.4 37.0 145-149 34.9802 37.0 37.0 37.0 25.0 37.0 150-151 34.8275 37.0 37.0 37.0 25.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.0 1101 2 0.0 1101 3 0.0 1101 4 0.0 1101 5 0.0 1101 6 0.0 1101 7 0.0 1101 8 0.0 1101 9 0.0 1101 10-14 0.0 1101 15-19 0.0 1101 20-24 0.0 1101 25-29 0.0 1101 30-34 0.0 1101 35-39 0.0 1101 40-44 0.0 1101 45-49 0.0 1101 50-54 0.0 1101 55-59 0.0 1101 60-64 0.0 1101 65-69 0.0 1101 70-74 0.0 1101 75-79 0.0 1101 80-84 0.0 1101 85-89 0.0 1101 90-94 0.0 1101 95-99 0.0 1101 100-104 0.0 1101 105-109 0.0 1101 110-114 0.0 1101 115-119 0.0 1101 120-124 0.0 1101 125-129 0.0 1101 130-134 0.0 1101 135-139 0.0 1101 140-144 0.0 1101 145-149 0.0 1101 150-151 0.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 18 1.0 19 0.0 20 1.0 21 0.0 22 1.0 23 2.0 24 4.0 25 9.0 26 10.0 27 14.0 28 21.0 29 21.0 30 34.0 31 45.0 32 59.0 33 123.0 34 159.0 35 442.0 36 2871.0 37 183.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.556167125344004 13.610207655741807 7.180385288966725 37.65323992994746 2 17.675 13.225000000000001 36.5 32.6 3 17.05 17.95 29.125 35.875 4 21.65 23.875 25.474999999999998 28.999999999999996 5 26.6 25.825 25.15 22.425 6 22.45 33.6 22.025 21.925 7 15.625 29.725 38.9 15.75 8 16.75 27.700000000000003 32.5 23.05 9 18.6 24.625 33.425 23.35 10-14 19.775000000000002 29.555 27.88 22.79 15-19 19.595000000000002 27.750000000000004 27.515 25.14 20-24 19.09 27.88 28.465 24.565 25-29 20.015 28.57 27.875 23.54 30-34 20.09 29.060000000000002 26.325 24.525 35-39 20.305 28.435 26.88 24.38 40-44 19.79 26.950000000000003 28.43 24.83 45-49 20.7 27.29 27.825 24.185000000000002 50-54 20.655 28.065 27.650000000000002 23.630000000000003 55-59 20.5 27.500000000000004 27.185 24.815 60-64 20.169999999999998 28.155 27.04 24.635 65-69 20.72 27.98 27.375 23.925 70-74 20.419999999999998 27.21 28.725 23.645 75-79 20.89 27.229999999999997 27.284999999999997 24.595 80-84 21.765 27.389999999999997 27.425 23.419999999999998 85-89 21.04 27.700000000000003 28.310000000000002 22.95 90-94 21.23 26.919999999999998 28.21 23.64 95-99 21.075 28.050000000000004 26.99 23.885 100-104 21.33 27.095000000000002 27.725 23.849999999999998 105-109 20.91 27.544999999999998 27.18 24.365000000000002 110-114 20.93 28.625 26.884999999999998 23.56 115-119 21.48 27.639999999999997 26.900000000000002 23.98 120-124 21.584999999999997 27.6 27.18 23.635 125-129 21.825 27.755000000000003 26.174999999999997 24.245 130-134 21.83 28.38 25.505 24.285 135-139 21.465 28.08 26.200000000000003 24.255 140-144 21.705 28.405 26.08 23.810000000000002 145-149 21.43 28.175 26.365 24.03 150-151 22.9375 28.1875 24.775 24.099999999999998 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.5 4 1.0 5 0.5 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.5 14 0.5 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.5 21 2.0 22 2.0 23 3.5 24 3.0 25 2.0 26 4.0 27 6.5 28 5.5 29 3.0 30 10.5 31 18.5 32 22.5 33 33.0 34 37.5 35 46.0 36 81.5 37 104.5 38 113.5 39 149.5 40 180.0 41 209.0 42 241.5 43 245.5 44 243.5 45 250.5 46 257.5 47 291.5 48 292.0 49 240.0 50 185.5 51 134.0 52 118.5 53 102.0 54 73.5 55 59.0 56 42.0 57 34.5 58 33.5 59 27.5 60 25.0 61 17.0 62 9.5 63 4.5 64 3.5 65 3.0 66 0.5 67 0.5 68 1.5 69 2.0 70 2.5 71 3.5 72 3.5 73 3.0 74 3.0 75 2.5 76 1.0 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.5 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.075 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150-151 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 60.425 #Duplication Level Percentage of deduplicated Percentage of total 1 63.50848158874638 38.375 2 20.893669838642946 25.25 3 8.688456764584195 15.75 4 3.6822507240380635 8.9 5 1.696317749275962 5.125 6 0.6206040546131568 2.25 7 0.3723624327678941 1.575 8 0.2896152254861398 1.4000000000000001 9 0.2068680182043856 1.125 >10 0.04137360364087712 0.25 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CCACTGAGGTACTTTTCATAGATGGAGAACTGAAGCCGACCATCAGTTCC 10 0.25 No Hit GTCAGGGTTGTAACCAACCTTCTTCAGGTAGGAAGAAACTTCCTTCACGA 9 0.22499999999999998 No Hit CTAGTAACAGAACCATATCCTGCAGCCTCTACTGTGGCCTAACCCTATGG 9 0.22499999999999998 No Hit GCGACCAAGCCTAGGTTTTGCTTATATAGAGAGTAGAGGCCCAAAGGTTT 9 0.22499999999999998 No Hit GGTGGAAGTGAAAGGGAACTCTTTGAGTGGATCCCAAATGTCGAATGCGG 9 0.22499999999999998 No Hit CTGCGGTACATGGCTCAGAATCATCTTAAAAGGAAAAAGTTGGGTGAGTT 9 0.22499999999999998 No Hit GCTCTCTTCACTTTGCGCTCGCATCCTTCACAGTCTATGCGCACCTTGAC 8 0.2 No Hit ATCGACGGTTGCTAGAGCCCCTTTTAACCAGTCCCACTCAGATGAATCGT 8 0.2 No Hit CCATCGTTCATGAAAGGGCCGCTCTATGACAGACACTTGGTTGACTGCCA 8 0.2 No Hit CTTTGCTTTGTGGCGGCCATGCCTTCGTATTCATCCTGCTTTGACAGCGA 8 0.2 No Hit GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGGTCCAATCTCGGTT 8 0.2 TruSeq Adapter, Index 20 (97% over 37bp) AGCACATCTAAGGAAGGGACAAAGCACAAATAAAGCATCGTTCCTCAAAA 8 0.2 No Hit GCCTTCATGGTGGATACTTCACTTCTCAGCAAAATATGTTATGCCTTATT 8 0.2 No Hit GCTTGCACAAGCAAAAGCTTGTCATTCGGAGAAGACCTCCCCATCACTGA 7 0.17500000000000002 No Hit GGCTCAAGAATCTGTAGGCAAGAAAGTGTCACCGTCCCATAACGTTTCGA 7 0.17500000000000002 No Hit CCCACTGCTTCAAACTTTCAAAAACGAATAACAACAATGTCCTAGCACTA 7 0.17500000000000002 No Hit GTGGCGGAGAGGGTGGAGGTGACTGTGGAATAACAACTGGTGGTGGAGGT 7 0.17500000000000002 No Hit CTGGCACTGTCCCTGATGCAATCATCCTGCCACCGCTCTTGAAAGAGTAG 7 0.17500000000000002 No Hit GTCCACAAGAAGCGCTGCCCCGTATCTTGGTGTCGGCTGGAAGTAGCATC 7 0.17500000000000002 No Hit GATCTCTTTCGGCTTCGTCTCCGCCGCTCCTTCACCACCGCTAACGGTGG 7 0.17500000000000002 No Hit GTCAATTGATCCGCCTGAAACCTTCTTCGGAGCAGGTTCTGTCTTTTCAA 7 0.17500000000000002 No Hit GCTTTTATGAGGAAAAAACAATAAAAAGAAAAAGGGTAGGTGGGAGGGGG 7 0.17500000000000002 No Hit GTGTCGGTAGATGTGATTGTGGTGAAGACTTCCTCAATCTCCTTATCATC 6 0.15 No Hit GTCACTTGAATGTGCCCTCACTAGAAAAACTGATGGTAAGGTCACGAGGA 6 0.15 No Hit CTTCACTTTTTGCACGATGTACCTCTCATTATTCATCAAGAAGACAGAGC 6 0.15 No Hit CTATGGAGAGTTTAAAGATAAATATCCCAGAAAACGAACAGGGACCCGCA 6 0.15 No Hit CTCTTTCATAAACTTCCCAGTGTTTTTCTCCATGAATCCTGTCAGCGATA 6 0.15 No Hit GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGGTCCAATCTCGGGT 6 0.15 TruSeq Adapter, Index 20 (97% over 37bp) CTCCTGAATCCCTCGAAAAATCACTCTCAAAAGAGTACATGTATAAACCC 6 0.15 No Hit GTATTAAAGGAGTCACCGGGCTTAAAATAAGAGAAGGGAAATCGAGAATG 6 0.15 No Hit GGGTACAAAAGTTGCAGAAACTCACCCCTTGAAGACAGCTGAAACCAAGT 6 0.15 No Hit CAATCTTATTCAACCATGACCTGCTTCTTCCCCTGAAAACCCCACTTGAA 6 0.15 No Hit CCCATCAACTCAGATAGCAGAGATAAATCCAATCCATCTCTTTCTAATTT 6 0.15 No Hit CAGGAACTTGGAAAAGTTCCATTTGATGCTGTCCCCAAAAAGTCCACCCT 6 0.15 No Hit TTTCAGCACAGTCTTGTACCCCAGGGTGTATTTCCCACTCTTCATGACCA 6 0.15 No Hit GGAGAATCCAACAATGGCACCAGAGACTGGCAAAGAGAACCCAGCTCCAG 6 0.15 No Hit CGGGTCGGAAATCTGTTTGGGAGAAGACAAGGAAGAGGAACTTTTCCGAA 6 0.15 No Hit CAGTGGTTGAATTTTGTAAAATCATAGCTTTTTCTGGCTGCATATTCTTT 5 0.125 No Hit CTCATTCCAGGTATCCTCAGAGATTGCAAATGCTCCGTCGGCCTTTGATT 5 0.125 No Hit TCTGCCTGTGAAGCAATCACCTTCTGAGTTTTTCCAACAGTCAGGTCATT 5 0.125 No Hit CTGAATGAGGGGGCATGCCCATTGCAAGCTCTGGCTGTGGACAAGTTGGA 5 0.125 No Hit CCCAGCCGATCGAAACCAAAGTCGAGAATATATTCCATAATCTCGAACTG 5 0.125 No Hit GTTCAATTTCTTATTCCACCCCTTCTCATATAGTAATGGTCTGTCAAACA 5 0.125 No Hit AGGCAAGAATCTTGTCAGTGGCCAAAACACCTCGCAGTTTTGGCAAAAGT 5 0.125 No Hit AGCTGCTGCAAGAATGTCATTTTCTGTAATAACAGCGCCAACAGATTCAT 5 0.125 No Hit CTTACGAAAGAGATCTATCAAAATTGGATTAGGACAAGACTGTACAAAGA 5 0.125 No Hit CATCAACATTCCCTATTACCGTGGCCTTTTGTTGCCTTAAATCAACATCT 5 0.125 No Hit ACCTTCTTCTCTTGTTTCCTAGCCAACGATCTCGAGGAGCTTCCCTCTCG 5 0.125 No Hit GTACATATTGGTAAGCCTTGTGCTTGGATTTTACACGAGCATCGGCAGGA 5 0.125 No Hit CTCCTCCAAACTTAACATCCACGATCGGAACCAAACAACGGAACAACAAA 5 0.125 No Hit GCATTAAAAAAGACTATTAATAATAGCAATTCTGATAACTAATTAGCCAG 5 0.125 No Hit CTTCAGAGTTGCCAGGGACAAGCTTGAGTTGCATGTCAATCTTTCCGAAC 5 0.125 No Hit GCTCTCTCTTTCTTTATCTTCCTCCTCAATTACTGGAGCACTAACCGGAA 5 0.125 No Hit ACCAGCAGCTAGCATGACTCAAAGCCCATCACTCTTGAGCTCCTCCTCAA 5 0.125 No Hit CCGCGTTATTGACAGAGGTGCCAAGGAAAGCTTCAGCAATCTCACGCATT 5 0.125 No Hit GAATGAACTTCTTCCTCATGAATCATGCTGAGAGTCGGCCCTGCCTCTTG 5 0.125 No Hit GCCCGGACCAAATGAACTGTTTCGTTCATCAAGCAAATCTCTCCATTCTT 5 0.125 No Hit GTGGCTGGGTGCTGCTGGATGAAGAAGGCGCCTCTGTGGGGTTGGTCGGC 5 0.125 No Hit CTCTCGACCTCCAGAGTGATGGTCTTTCCGGTCAAAGTCTTGACAAAAAT 5 0.125 No Hit GCTTGAAAGGAGGGACGCGCGGCGCTGGCACTGACCCGGATACTATTTGA 5 0.125 No Hit GGGCAGGCATGTCAGTATCATCACCAGCAGCCTCATCCTCATCAATGCTA 5 0.125 No Hit CCTATTTCTATCCAATAAAGTTTTTGCTAATAGAATTCTTCACTAATTTG 5 0.125 No Hit GTTGTTTTCTTTCTACATACATTTCCAAATTTCTCTACAAGCGGCTTCAT 5 0.125 No Hit CCACACTTTGCCGCTCACATCTTCCAAGTTAAGCAATACACCTTTAGTAC 5 0.125 No Hit ACTGGGAGAAACCCTAAAAAGAAAAATCAGGTGATGGTATTAAGAGAGAA 5 0.125 No Hit GTTCCGGATAAGAACGCCTTTCCACTCCAACAATGAACCTGCTCTCATTG 5 0.125 No Hit GTCTTCATCCTCACTTTCCTCCTTAACTGGTTCCTTCTTCTCCTCAGCGG 5 0.125 No Hit GTCGGAGATTGAATTTCCATAGATCCAGTACCCAATTAGAGCAACAGGGA 5 0.125 No Hit CACTGTAGAAACATCCACACCAAGTGTCAGCATGGCATCCAAGTCATTCT 5 0.125 No Hit GCTGTTTTCAATCATGGACTCAGTGAAAATATTGTTTTCCTCGTTTTCAT 5 0.125 No Hit CTTGGATGGTTGGTAGACAAGAAGTCCTGTGATACCATTGGAGTGTCTAG 5 0.125 No Hit GCTCGGATACATGAAAACTCTAACACGAGTTCCCATGGACTTTGGAGGCA 5 0.125 No Hit TCGCTTCCCATTCTCTGAACAGTACTTCACCACGGGCAGCGCGTCAATAA 5 0.125 No Hit GCCAGCTGAATATAAGCCTGGGCTCTTCTGTAGAGAGCCTTCACATTTCT 5 0.125 No Hit ACTTTTCTGATCATAATCTCCAGATAGTTTGATGATCTGCATTTCTTGAT 5 0.125 No Hit ACAAATTCTTGAACAATAAGAACGCTTGGATTGCTGATGCAGTGCACTCA 5 0.125 No Hit CCGGGAGTATCCATCTCGTAATCCAAATGAGGGGGCAGGACCAAATCTCC 5 0.125 No Hit CGCTGGATTGTTGATCTGCTGCTGAGCAGCTACAGTCCTAAGAACCTCCA 5 0.125 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.025 0.0 0.0 0.0 0.0 58-59 0.05 0.0 0.0 0.0 0.0 60-61 0.05 0.0 0.0 0.0 0.0 62-63 0.05 0.0 0.0 0.0 0.0 64-65 0.05 0.0 0.0 0.0 0.0 66-67 0.05 0.0 0.0 0.0 0.0 68-69 0.07500000000000001 0.0 0.0 0.0 0.0 70-71 0.1 0.0 0.0 0.0 0.0 72-73 0.1375 0.0 0.0 0.0 0.0 74-75 0.15 0.0 0.0 0.0 0.0 76-77 0.225 0.0 0.0 0.0 0.0 78-79 0.30000000000000004 0.0 0.0 0.0 0.0 80-81 0.4 0.0 0.0 0.0 0.0 82-83 0.42500000000000004 0.0 0.0 0.0 0.0 84-85 0.5125 0.0 0.0 0.0 0.0 86-87 0.6125 0.0 0.0 0.0 0.0 88-89 0.6875 0.0 0.0 0.0 0.0 90-91 0.7625 0.0 0.0 0.0 0.0 92-93 0.9625 0.0 0.0 0.0 0.0 94-95 1.1 0.0 0.0 0.0 0.0 96-97 1.25 0.0 0.0 0.0 0.0 98-99 1.3875000000000002 0.0 0.0 0.0 0.0 100-101 1.6375 0.0 0.0 0.0 0.0 102-103 2.0125 0.0 0.0 0.0 0.0 104-105 2.4749999999999996 0.0 0.0 0.0 0.0 106-107 3.0375 0.0 0.0 0.0 0.0 108-109 3.575 0.0 0.0 0.0 0.0 110-111 3.975 0.0 0.0 0.0 0.0 112-113 4.4 0.0 0.0 0.0 0.0 114-115 4.9625 0.0 0.0 0.0 0.0 116-117 5.2125 0.0 0.0 0.0 0.0 118-119 5.9125 0.0 0.0 0.0 0.0 120-121 6.475 0.0 0.0 0.0 0.0 122-123 7.0 0.0 0.0 0.0 0.0 124-125 7.575 0.0 0.0 0.0 0.0 126-127 8.025 0.0 0.0 0.0 0.0 128-129 8.35 0.0 0.0 0.0 0.0 130-131 8.8 0.0 0.0 0.0 0.0 132-133 9.587499999999999 0.0 0.0 0.0 0.0 134-135 10.3125 0.0 0.0 0.0 0.0 136-137 11.0625 0.0 0.0 0.0 0.0 138-139 11.6875 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content pass >>END_MODULE SRR26075347 read2 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR26075347_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.14825 37.0 37.0 37.0 37.0 37.0 2 36.228 37.0 37.0 37.0 37.0 37.0 3 36.143 37.0 37.0 37.0 37.0 37.0 4 36.299 37.0 37.0 37.0 37.0 37.0 5 36.277 37.0 37.0 37.0 37.0 37.0 6 36.308 37.0 37.0 37.0 37.0 37.0 7 36.1935 37.0 37.0 37.0 37.0 37.0 8 36.254 37.0 37.0 37.0 37.0 37.0 9 36.2565 37.0 37.0 37.0 37.0 37.0 10-14 36.248599999999996 37.0 37.0 37.0 37.0 37.0 15-19 36.1605 37.0 37.0 37.0 37.0 37.0 20-24 36.1262 37.0 37.0 37.0 37.0 37.0 25-29 36.0225 37.0 37.0 37.0 37.0 37.0 30-34 35.8706 37.0 37.0 37.0 37.0 37.0 35-39 35.8391 37.0 37.0 37.0 37.0 37.0 40-44 35.7671 37.0 37.0 37.0 37.0 37.0 45-49 35.778499999999994 37.0 37.0 37.0 37.0 37.0 50-54 35.6689 37.0 37.0 37.0 37.0 37.0 55-59 35.6559 37.0 37.0 37.0 37.0 37.0 60-64 35.6486 37.0 37.0 37.0 37.0 37.0 65-69 35.5736 37.0 37.0 37.0 37.0 37.0 70-74 35.5729 37.0 37.0 37.0 37.0 37.0 75-79 35.41330000000001 37.0 37.0 37.0 37.0 37.0 80-84 35.405 37.0 37.0 37.0 37.0 37.0 85-89 35.467 37.0 37.0 37.0 37.0 37.0 90-94 35.37515 37.0 37.0 37.0 37.0 37.0 95-99 35.4279 37.0 37.0 37.0 37.0 37.0 100-104 35.3031 37.0 37.0 37.0 34.6 37.0 105-109 35.3191 37.0 37.0 37.0 34.6 37.0 110-114 35.2813 37.0 37.0 37.0 34.6 37.0 115-119 35.2197 37.0 37.0 37.0 32.2 37.0 120-124 35.07695 37.0 37.0 37.0 27.4 37.0 125-129 35.07055 37.0 37.0 37.0 27.4 37.0 130-134 35.031000000000006 37.0 37.0 37.0 27.4 37.0 135-139 34.8959 37.0 37.0 37.0 25.0 37.0 140-144 34.84325 37.0 37.0 37.0 25.0 37.0 145-149 34.84555 37.0 37.0 37.0 25.0 37.0 150-151 34.45075 37.0 37.0 37.0 25.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.0 1101 2 0.0 1101 3 0.0 1101 4 0.0 1101 5 0.0 1101 6 0.0 1101 7 0.0 1101 8 0.0 1101 9 0.0 1101 10-14 0.0 1101 15-19 0.0 1101 20-24 0.0 1101 25-29 0.0 1101 30-34 0.0 1101 35-39 0.0 1101 40-44 0.0 1101 45-49 0.0 1101 50-54 0.0 1101 55-59 0.0 1101 60-64 0.0 1101 65-69 0.0 1101 70-74 0.0 1101 75-79 0.0 1101 80-84 0.0 1101 85-89 0.0 1101 90-94 0.0 1101 95-99 0.0 1101 100-104 0.0 1101 105-109 0.0 1101 110-114 0.0 1101 115-119 0.0 1101 120-124 0.0 1101 125-129 0.0 1101 130-134 0.0 1101 135-139 0.0 1101 140-144 0.0 1101 145-149 0.0 1101 150-151 0.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 13 4.0 14 7.0 15 12.0 16 9.0 17 7.0 18 2.0 19 4.0 20 3.0 21 5.0 22 6.0 23 11.0 24 9.0 25 16.0 26 12.0 27 22.0 28 18.0 29 19.0 30 30.0 31 27.0 32 53.0 33 108.0 34 232.0 35 721.0 36 2455.0 37 208.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 42.010502625656414 23.830957739434858 11.002750687671918 23.15578894723681 2 30.175 26.424999999999997 26.950000000000003 16.45 3 23.200000000000003 26.75 31.424999999999997 18.625 4 26.8 31.574999999999996 22.6 19.025 5 27.55 35.175 19.1 18.175 6 22.025 38.3 21.349999999999998 18.325 7 22.8 22.025 36.7 18.475 8 23.400000000000002 26.150000000000002 26.25 24.2 9 23.95 24.975 27.025 24.05 10-14 24.055 30.775000000000002 24.34 20.830000000000002 15-19 24.72 28.51 26.88 19.89 20-24 24.755 28.24 26.665 20.34 25-29 24.965 28.975 25.724999999999998 20.335 30-34 24.465 27.944999999999997 26.740000000000002 20.849999999999998 35-39 25.305 27.865000000000002 26.974999999999998 19.855 40-44 23.865 28.23 26.97 20.935000000000002 45-49 25.240000000000002 28.4 25.72 20.64 50-54 23.57 28.585 27.315 20.53 55-59 25.405 27.529999999999998 27.694999999999997 19.37 60-64 24.38 28.005000000000003 27.595 20.02 65-69 25.695 26.974999999999998 26.974999999999998 20.355 70-74 24.015 29.03 26.355 20.599999999999998 75-79 23.775 28.854999999999997 26.68 20.69 80-84 24.345 28.865000000000002 27.060000000000002 19.73 85-89 24.67 28.044999999999998 27.029999999999998 20.255000000000003 90-94 25.136256812840642 28.15640782039102 26.911345567278367 19.795989799489973 95-99 25.650000000000002 27.625 26.305 20.419999999999998 100-104 25.045 27.76 27.08 20.115 105-109 25.86 28.09 25.75 20.3 110-114 26.340000000000003 27.93 26.119999999999997 19.61 115-119 26.38 28.515 25.395 19.71 120-124 25.146257312865643 28.091404570228512 26.936346817340866 19.82599129956498 125-129 26.846342317115855 28.421421071053555 25.306265313265662 19.425971298564928 130-134 26.640000000000004 28.01 25.929999999999996 19.42 135-139 26.37027405481096 28.440688137627525 25.980196039207843 19.208841768353672 140-144 26.171542885721433 27.94698674668667 26.466616654163538 19.414853713428357 145-149 26.83670917729432 27.701925481370342 26.186546636659163 19.274818704676168 150-151 26.469117279319832 28.14453613403351 26.04401100275069 19.342335583895974 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.5 5 1.0 6 1.5 7 1.0 8 1.0 9 1.5 10 2.0 11 1.5 12 0.0 13 0.0 14 0.5 15 1.0 16 1.5 17 1.0 18 0.0 19 0.5 20 1.5 21 1.5 22 1.5 23 3.5 24 3.5 25 1.5 26 3.5 27 8.0 28 7.5 29 8.0 30 9.5 31 15.0 32 19.0 33 32.5 34 36.5 35 38.5 36 61.5 37 81.0 38 108.5 39 156.0 40 205.0 41 224.0 42 260.5 43 284.0 44 274.0 45 271.0 46 265.0 47 262.5 48 238.0 49 201.5 50 161.5 51 131.0 52 124.5 53 104.5 54 72.5 55 61.5 56 50.5 57 29.5 58 21.0 59 18.0 60 14.0 61 14.0 62 11.0 63 7.0 64 5.0 65 1.0 66 1.5 67 3.0 68 3.0 69 1.5 70 2.0 71 2.0 72 1.0 73 1.0 74 2.5 75 2.0 76 0.5 77 1.0 78 0.5 79 1.0 80 3.0 81 5.5 82 5.5 83 2.5 84 4.0 85 4.0 86 3.0 87 2.5 88 0.5 89 0.5 90 0.5 91 1.5 92 2.0 93 1.0 94 1.0 95 1.0 96 0.5 97 0.5 98 0.0 99 0.5 100 7.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.025 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.005 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.005 125-129 0.005 130-134 0.0 135-139 0.02 140-144 0.025 145-149 0.025 150-151 0.025 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 61.550000000000004 #Duplication Level Percentage of deduplicated Percentage of total 1 65.59707554833469 40.375 2 19.65881397238018 24.2 3 7.920389926888709 14.625 4 3.9805036555645814 9.8 5 1.462225832656377 4.5 6 0.487408610885459 1.7999999999999998 7 0.3249390739236393 1.4000000000000001 8 0.2843216896831844 1.4000000000000001 9 0.16246953696181965 0.8999999999999999 >10 0.12185215272136475 1.0 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG 19 0.475 No Hit GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGT 11 0.27499999999999997 No Hit CAGGACTTCACAGTTGGTCTAGAGGCCTTCTCTCGAATAGCTCCAGCAGT 10 0.25 No Hit AAATGACTTGCAAGAATACTTAAAAATGTCAATTGTAACATGTTTATATC 9 0.22499999999999998 No Hit ATTTCAAGGAGATCATTAGTTTTTTGGGGTCATTTTCACTCAACAAGCCG 9 0.22499999999999998 No Hit CTCCAAGGATGGCCAGACCCGTGAACATGCCTTGCTTGCCTTCACCCTTG 9 0.22499999999999998 No Hit CCCCGATTTCCTCCTCCTCCTCTGTATTTTAGGCAGCCAAATGGTCCTTC 9 0.22499999999999998 No Hit AACCACCACAACCGAACTTCTCTCGTCCTTATCTCCATTCTTCCATAGCT 8 0.2 No Hit CTTGCCACTACGATCCATATGGCCCTATCTACAATGATCGTGAGCGTGTG 8 0.2 No Hit TGATTATTGATACTATGCCGGACTCACAGAAGCAGGAGAGAAGATACCTT 8 0.2 No Hit ATTGAGTACTCAAGATTTGGTTTGCACCCTCAGTACATTTCTTGAAAGAT 8 0.2 No Hit AAAGAATGGTTTTCGAAGAAGGTGCTTGTCCATGGCAAAGCAGCAAAAGA 8 0.2 No Hit GGATACTGCATAATATCTCACAGTTGGCAGAGGCCATTTTGTTCAGATGT 8 0.2 No Hit CTATCATTTAATTGCCTAACTTATTAAAACCTTTTGAGATATTTCTGTCT 8 0.2 No Hit ACTTGTTGGATTCACTCTGTCTTGTTTAGCTACCAGGAAAGAAGAGAAAA 7 0.17500000000000002 No Hit GTTTGCTTCTCTCGATTTATGCATGAGAGATTTGGATTGGAGATGGGTGG 7 0.17500000000000002 No Hit GGACAAAGTTCCAGCTGATGAACAACAACTAGCTCAATGGGTGATTCCTC 7 0.17500000000000002 No Hit AGATTGGCCCAAGATTTCTCACATCCCAGACATAGATATCAACTGGGATA 7 0.17500000000000002 No Hit CATGCTCTCAGGGAAGATTCCGGCAAGTATTTTGACAGGAGGAATTGTTT 7 0.17500000000000002 No Hit TTTCCTTAAAGAAGGCAGAAAGCAAACAGAAGAAAGATCACAAATAGCCC 7 0.17500000000000002 No Hit ACCGACATGAATCTGGAAAGCGTGCACCCAGACAGCATAGACTATGGTGC 7 0.17500000000000002 No Hit AAGAGGAAGAGATGGCAGAGCAGGAGGTGAAGAAGGTAGAGGCTGTAACG 7 0.17500000000000002 No Hit CGTAGAAACTCAATCAAGAACCTCAAGATCAACCCTTTAAATCCGGTATT 6 0.15 No Hit CGAGCCTTCATTTTGTCTGCAGAAACCTCTCGCATAACTACCATGGTGGC 6 0.15 No Hit CAACAATAATCCTGATCTGGAAAGCATGAGCAAGTATGGTCAGGAACTAC 6 0.15 No Hit AATCATTACAACTCTCTTGTCAACCCACGCCGCTCAACAATTGGTGCAGG 6 0.15 No Hit GTTGGAGAGTAATTTGGAAGTGAAGGCAAAGATTTATGGGGATACTGCCT 6 0.15 No Hit CTGAATTACAATCTCGGGCACTTGAGCAAGTCAACTTGTCGCCTAGGACC 6 0.15 No Hit AAAAATGTCGGGCGGGTTTTTCAGGGGCACTTCTGCTGATCAGGACACTC 6 0.15 No Hit GGAGCACAAGAAGAAGGTCGAGTCCAAGAACTCTTTGGAGAACTATGCCT 6 0.15 No Hit GTTTGTTAGATAATGAGATGTTTGGTCAAATTCTATGAACTTCCTTGTCT 6 0.15 No Hit TGTGAATCTAATCGGTTTACTGAGATTGACTCAACAGTTAGAACACCTAC 6 0.15 No Hit CTCGGAAATTAGCTTCTCTGTCCTTCCTATGTTATTGTATTTGAGTTGCA 6 0.15 No Hit CTTTAAAGGTCACCCTTCAATATTCCATGCCTTTGTGATTTCTGTCGTCT 6 0.15 No Hit TGAAAGCGCGTGAACAGCTTTTTGAGAAAGCTGTGACTCCGAGTGATGTT 5 0.125 No Hit TATGGCTGGACAGCTAACATGGAGAGGATCATGAAGGCTCAGGCATTGAG 5 0.125 No Hit GGAAGAGAATGCAATCAAACAGAGAATCTGCAAGGAGATCCAGGATGAAA 5 0.125 No Hit GCTGACTATGTTGAAGTCATTAGCAAGCACAACGAAGACAAACAGTATGT 5 0.125 No Hit GCTGAAACATTAGTACCAGTTTGGATGGAGGCCCTCGTTGGTGCTGCTTC 5 0.125 No Hit GGAAGGCATCCCCCCAGACCAGCAGAGGTTGATTTTTGCTGGGAAGCAGC 5 0.125 No Hit CGACGATGAACCGCCAAATCAGAAGTATATTCAGGAGATATTGAGTTTGA 5 0.125 No Hit AAGGTGGCCAACTCGTCCACAACTCAGCCAACCTCCTCTCCTCTAGTGTT 5 0.125 No Hit GAATCTCGTCTGATCCTGGATTTCACTGATCACGTTTGGACCGAGTGCTT 5 0.125 No Hit ACACCAAGGAAGAAGTTGCATGGTATGCTGGTAAGCGCATGGCATACATC 5 0.125 No Hit TTCTGCAACTCCATGGACATATCTTCGTGTTTATTCAGCATTGTTGCTGG 5 0.125 No Hit CTAAGCTTTACAAAGAAAACACATCAATCTCTCTCCTTTTCTTCTCTCCA 5 0.125 No Hit GGTAAAGCCATGGATAAAAACAAGTCTAGCCCCAGGCTCCGGAGTTGTTA 5 0.125 No Hit TGAGAAACTAATGGCGTTGGGTTCAAGAAACTTCTTTCTGTGTCCAAAAA 5 0.125 No Hit GATTCAGCAGCCAAGGTTACAGGCATGCTTCTAGAGATGGATCAAACTGA 5 0.125 No Hit ATTTTCTTTGAGAGTGCATAGATTTGTGTTGATATAGAAAACAATGGCAC 5 0.125 No Hit TGATCTCAGGCAGCAGAAACAAACACGTGAAATCTCCGAGGATGGGAGTG 5 0.125 No Hit AGAACATCTCCATCACTGCAGAAAAGATTGCAACATTGGTGAAAGCAGCC 5 0.125 No Hit GTTTCATAATAGCCGATGGCTTCTTTGAAGACTGTGCTTGTGCCCCTTGT 5 0.125 No Hit GTTCAAGGCTGGAAAATATGCAAAGGCATCTAAAAGATATGAGAAGGCTG 5 0.125 No Hit ACTGAAGAGGAACATAGGATGTTTCTACTTGGTCTGCAGAAGCTTGGTAA 5 0.125 No Hit GCCTTATCCCTGGGTGCCGTGTGCACCTTATGTTGTTAAGCCACAAGGGT 5 0.125 No Hit GTTGAAAGTAGTGCTCTGAATCCGACTGCCCCAAGTTCGGAAGAAATATT 5 0.125 No Hit GAATTCTTCCACATGTTGACAACTTCGCCCACATTGGAGGTTTCATGAGT 5 0.125 No Hit AATCAGCGATCCTTCAGTTCAGAGTGATATCAAGCTCTGGCCATTCAAGG 5 0.125 No Hit GGGTCTTCTACTACTTGAGATTTGGGGCGATCAAGATTTTCTTGAAGAGA 5 0.125 No Hit ACGATTGCTTGGTCGGCTGCTGTTGATAAGGGTGTTCAGCCAGATGTGCA 5 0.125 No Hit TTATGTTCTTGTATGAACTGAGATAGCAAGAATTGGATATACTTGCCTTC 5 0.125 No Hit TGGGCAGCAAGCGGCCTTTGCTCTCTCTTCCCTTCTTCATGGACAGGGAA 5 0.125 No Hit AGGGCGTATACTAGAAGCTGTGGACGAAAGGCTGAACAATGACTATGTTG 5 0.125 No Hit ACTCGCAGTGCGAGAATAAAAAAATGCCTTTTGTTTCCCAGATAAAAAGA 5 0.125 No Hit GGAACTGGCACAGTAATCCTTCAACTTCAACAGTCTTAAAAAGCAAACGC 5 0.125 No Hit CTTTCTCGAATTCCTCCCCGCTCTCTCTTCCTCATCCAGAACAGACGCCC 5 0.125 No Hit ACTCCACCCGTGGCATCGCCTTCACTGGAAAATGATTTCTCGAAACAAAG 5 0.125 No Hit GTTTCCTAGCTGGAGCAGCTACAACAGCAGCTGGAAGATTAGTAGCCCAA 5 0.125 No Hit GCTGGCCTCAGTGAGATTTCATTAGAGTACTCCCAAAGAGTTAGAGAAGT 5 0.125 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.025 0.0 0.0 0.0 0.0 58-59 0.05 0.0 0.0 0.0 0.0 60-61 0.05 0.0 0.0 0.0 0.0 62-63 0.05 0.0 0.0 0.0 0.0 64-65 0.05 0.0 0.0 0.0 0.0 66-67 0.05 0.0 0.0 0.0 0.0 68-69 0.07500000000000001 0.0 0.0 0.0 0.0 70-71 0.1 0.0 0.0 0.0 0.0 72-73 0.1375 0.0 0.0 0.0 0.0 74-75 0.15 0.0 0.0 0.0 0.0 76-77 0.225 0.0 0.0 0.0 0.0 78-79 0.30000000000000004 0.0 0.0 0.0 0.0 80-81 0.4 0.0 0.0 0.0 0.0 82-83 0.4375 0.0 0.0 0.0 0.0 84-85 0.5375000000000001 0.0 0.0 0.0 0.0 86-87 0.6375 0.0 0.0 0.0 0.0 88-89 0.7375 0.0 0.0 0.0 0.0 90-91 0.8125 0.0 0.0 0.0 0.0 92-93 1.0125 0.0 0.0 0.0 0.0 94-95 1.15 0.0 0.0 0.0 0.0 96-97 1.2999999999999998 0.0 0.0 0.0 0.0 98-99 1.4375 0.0 0.0 0.0 0.0 100-101 1.6875 0.0 0.0 0.0 0.0 102-103 2.0625 0.0 0.0 0.0 0.0 104-105 2.5250000000000004 0.0 0.0 0.0 0.0 106-107 3.0875 0.0 0.0 0.0 0.0 108-109 3.625 0.0 0.0 0.0 0.0 110-111 4.0375 0.0 0.0 0.0 0.0 112-113 4.4875 0.0 0.0 0.0 0.0 114-115 5.0875 0.0 0.0 0.0 0.0 116-117 5.3375 0.0 0.0 0.0 0.0 118-119 6.0875 0.0 0.0 0.0 0.0 120-121 6.65 0.0 0.0 0.0 0.0 122-123 7.175 0.0 0.0 0.0 0.0 124-125 7.800000000000001 0.0 0.0 0.0 0.0 126-127 8.3 0.0 0.0 0.0 0.0 128-129 8.6875 0.0 0.0 0.0 0.0 130-131 9.149999999999999 0.0 0.0 0.0 0.0 132-133 9.9375 0.0 0.0 0.0 0.0 134-135 10.6875 0.0 0.0 0.0 0.0 136-137 11.524999999999999 0.0 0.0 0.0 0.0 138-139 12.1875 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content pass >>END_MODULE Read 1115454 spots for SRR26075347.sra Written 1115454 spots for SRR26075347.sra Read 1115454 spots for SRR26075347.sra Written 1115454 spots for SRR26075347.sra Read 1115454 spots for SRR26075347.sra Written 1115454 spots for SRR26075347.sra Read 1115454 spots for SRR26075347.sra Written 1115454 spots for SRR26075347.sra Read 1115454 spots for SRR26075347.sra Written 1115454 spots for SRR26075347.sra Read 1115454 spots for SRR26075347.sra Written 1115454 spots for SRR26075347.sra Read 1115454 spots for SRR26075347.sra Written 1115454 spots for SRR26075347.sra Read 1115454 spots for SRR26075347.sra Written 1115454 spots for SRR26075347.sra Read 1115454 spots for SRR26075347.sra Written 1115454 spots for SRR26075347.sra Read 1115454 spots for SRR26075347.sra Written 1115454 spots for SRR26075347.sra Read 1115454 spots for SRR26075347.sra Written 1115454 spots for SRR26075347.sra Read 1115469 spots for SRR26075347.sra Written 1115469 spots for SRR26075347.sra Read 1115454 spots for SRR26075347.sra Written 1115454 spots for SRR26075347.sra Read 1115454 spots for SRR26075347.sra Written 1115454 spots for SRR26075347.sra Read 1115454 spots for SRR26075347.sra Written 1115454 spots for SRR26075347.sra Read 1115454 spots for SRR26075347.sra Written 1115454 spots for SRR26075347.sra Read 1115454 spots for SRR26075347.sra Written 1115454 spots for SRR26075347.sra Read 1115454 spots for SRR26075347.sra Written 1115454 spots for SRR26075347.sra Read 1115454 spots for SRR26075347.sra Written 1115454 spots for SRR26075347.sra Read 1115454 spots for SRR26075347.sra Written 1115454 spots for SRR26075347.sra SRR ids: ['SRR26075347.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_j3eywke6 SRR26075347.sra spots: 22309095 blocks: [[1, 1115454], [1115455, 2230908], [2230909, 3346362], [3346363, 4461816], [4461817, 5577270], [5577271, 6692724], [6692725, 7808178], [7808179, 8923632], [8923633, 10039086], [10039087, 11154540], [11154541, 12269994], [12269995, 13385448], [13385449, 14500902], [14500903, 15616356], [15616357, 16731810], [16731811, 17847264], [17847265, 18962718], [18962719, 20078172], [20078173, 21193626], [21193627, 22309095]] SRR26075347 file size 8234473 SRR26075347 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o SRR26075347 SRR26075347_1.fastq SRR26075347_2.fastq Input file: SRR26075347_1.fastq Paired file: SRR26075347_2.fastq trimmed: SRR26075347-trimmed-pair1.fastq, SRR26075347-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Wed Feb 12 02:35:27 2025 >> started Wed Feb 12 02:35:52 2025 >> done (25.056s) 22309095 read pairs processed; of these: 119 ( 0.00%) short read pairs filtered out after trimming by size control 90921 ( 0.41%) empty read pairs filtered out after trimming by size control 22218055 (99.59%) read pairs available; of these: 3545901 (15.96%) trimmed read pairs available after processing 18672154 (84.04%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 18 10 0.00% 19 9 0.00% 20 5 0.00% 21 18 0.00% 22 13 0.00% 23 18 0.00% 24 11 0.00% 25 25 0.00% 26 14 0.00% 27 25 0.00% 28 15 0.00% 29 29 0.00% 30 35 0.00% 31 36 0.00% 32 29 0.00% 33 38 0.00% 34 41 0.00% 35 46 0.00% 36 44 0.00% 37 37 0.00% 38 59 0.00% 39 66 0.00% 40 97 0.00% 41 61 0.00% 42 69 0.00% 43 88 0.00% 44 87 0.00% 45 129 0.00% 46 103 0.00% 47 147 0.00% 48 146 0.00% 49 167 0.00% 50 218 0.00% 51 260 0.00% 52 266 0.00% 53 345 0.00% 54 300 0.00% 55 323 0.00% 56 318 0.00% 57 416 0.00% 58 510 0.00% 59 575 0.00% 60 674 0.00% 61 847 0.00% 62 826 0.00% 63 1022 0.00% 64 1123 0.01% 65 1285 0.01% 66 1331 0.01% 67 1532 0.01% 68 1770 0.01% 69 2000 0.01% 70 2269 0.01% 71 2592 0.01% 72 2951 0.01% 73 3440 0.02% 74 3723 0.02% 75 4353 0.02% 76 4714 0.02% 77 4949 0.02% 78 5562 0.03% 79 6211 0.03% 80 6715 0.03% 81 7697 0.03% 82 8689 0.04% 83 9621 0.04% 84 10479 0.05% 85 11555 0.05% 86 12269 0.06% 87 13146 0.06% 88 14107 0.06% 89 14780 0.07% 90 16044 0.07% 91 17110 0.08% 92 18672 0.08% 93 20275 0.09% 94 21935 0.10% 95 23306 0.10% 96 24574 0.11% 97 25414 0.11% 98 26824 0.12% 99 27905 0.13% 100 28659 0.13% 101 29619 0.13% 102 31093 0.14% 103 33293 0.15% 104 34687 0.16% 105 36902 0.17% 106 38168 0.17% 107 39012 0.18% 108 40862 0.18% 109 40661 0.18% 110 41883 0.19% 111 43513 0.20% 112 44602 0.20% 113 46282 0.21% 114 48903 0.22% 115 49547 0.22% 116 50183 0.23% 117 52564 0.24% 118 53856 0.24% 119 55102 0.25% 120 55996 0.25% 121 56702 0.26% 122 56899 0.26% 123 58751 0.26% 124 61133 0.28% 125 62788 0.28% 126 64514 0.29% 127 65949 0.30% 128 66359 0.30% 129 68092 0.31% 130 69881 0.31% 131 70486 0.32% 132 71125 0.32% 133 72190 0.32% 134 73250 0.33% 135 74485 0.34% 136 77278 0.35% 137 77088 0.35% 138 79743 0.36% 139 81266 0.37% 140 81982 0.37% 141 82683 0.37% 142 82226 0.37% 143 83613 0.38% 144 84865 0.38% 145 87212 0.39% 146 86868 0.39% 147 88760 0.40% 148 90115 0.41% 149 90844 0.41% 150 93828 0.42% 151 18672154 84.04% 22218055 reads passed initial QC criterion=sequence-density sequence-density=0.25 sequence-density-rank=1 fanout-score=5.55 fanout-score-rank=23 prefix-density=0.41 prefix-fanout=3.5 sequence=TCCTTGTCCTGGATCTT criterion=fanout-score sequence-density=0.12 sequence-density-rank=21 fanout-score=84.41 fanout-score-rank=1 prefix-density=0.61 prefix-fanout=16.6 sequence=TTCTTCTTGTCATC criterion=sequence-density sequence-density=0.24 sequence-density-rank=1 fanout-score=2.28 fanout-score-rank=42 prefix-density=0.25 prefix-fanout=2.2 sequence=CCAGACCAGCAGAGGTTGAT criterion=fanout-score sequence-density=0.02 sequence-density-rank=39 fanout-score=534.99 fanout-score-rank=1 prefix-density=0.41 prefix-fanout=21.4 sequence=GAGAAGAAACACTAAAAAAATCACACAAGCGAAATCTCCAGCGAGAAGAGAAAGATGGATTTCAGAATCATGGGTCTTGACGCGCCACTCTTCAACACCCTCCAGCACATGATGGATGCAAGTGATCATGAGGCAGACAAGTCCTTCAATGCGCCAACACGCACTTACGTACGTGATGCCAAGGCAATGGCATCAACACCAGCTGATGTGAAAGAGTATCCAAACTCTTATGCGTTCGTCATTGACATGCCGGGACTGAAATCAGGGGACATCAAGGTTCAAGTGGA SRR26075347 testing PE reads STAR mapping to Ensembl genome Started job on | Feb 12 02:36:33 Started mapping on | Feb 12 02:36:34 Finished on | Feb 12 02:39:22 Mapping speed, Million of reads per hour | 476.10 Number of input reads | 22218055 Average input read length | 293 UNIQUE READS: Uniquely mapped reads number | 20287040 Uniquely mapped reads % | 91.31% Average mapped length | 292.03 Number of splices: Total | 16121793 Number of splices: Annotated (sjdb) | 15672169 Number of splices: GT/AG | 15855901 Number of splices: GC/AG | 195952 Number of splices: AT/AC | 18286 Number of splices: Non-canonical | 51654 Mismatch rate per base, % | 0.41% Deletion rate per base | 0.03% Deletion average length | 2.92 Insertion rate per base | 0.02% Insertion average length | 2.63 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 629090 % of reads mapped to multiple loci | 2.83% Number of reads mapped to too many loci | 46012 % of reads mapped to too many loci | 0.21% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 5.33% % of reads unmapped: other | 0.33% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 1301925 1301925 1301925 N_multimapping 629090 629090 629090 N_noFeature 535160 20070883 668219 N_ambiguous 212743 1202 129109 UnstrandedReadsAssigned:19539137 PositiveStrandReadsAssigned:214955 NegativeStrandReadsAssigned:19489712 Dataset is classified negative stranded MeadianReadLen=151 20thPercentileLength=151 echo kmer=147 SRR26075347 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,400 [index] number of k-mers: 62,057,036 [index] number of equivalence classes: 130,681 [quant] running in paired-end mode [quant] will process pair 1: SRR26075347-trimmed-pair1.fastq SRR26075347-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 22,218,055 reads, 19,845,359 reads pseudoaligned [quant] estimated average fragment length: 214.413 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,276 rounds 52401 SRR26075347.ke.tsv 34699 SRR26075347.se.tsv 87100 total ==> SRR26075347.ke.tsv <== target_id length eff_length est_counts tpm Potri.005G200100.1.v4.1 2018 1804.59 4752 116.391 Potri.005G024800.1.v4.1 1035 821.587 9705 522.11 Potri.004G059700.1.v4.1 961 747.602 1 0.0591221 Potri.007G009000.2.v4.1 1416 1202.59 0 0 Potri.003G141000.2.v4.1 2943 2729.59 990 16.0309 Potri.016G087400.1.v4.1 270 91.0399 1341 651.054 Potri.015G069301.1.v4.1 564 352.837 0 0 Potri.010G195200.1.v4.1 1773 1559.59 39 1.10529 Potri.012G127500.1.v4.1 977 763.602 4678 270.778 ==> SRR26075347.se.tsv <== Potri.001G166300.v4.1 0 Potri.001G448400.v4.1 16 Potri.001G233950.v4.1 0 Potri.001G122700.v4.1 89 Potri.001G212900.v4.1 0 Potri.001G182400.v4.1 0 Potri.001G256600.v4.1 0 Potri.001G040500.v4.1 3 Potri.001G416900.v4.1 0 Potri.001G452600.v4.1 117 SRR26075347 completed mapping pipeline successfully