Starting /dee2/code/volunteer_pipeline.sh SRR26075348 current disk space = 3049569755136 free memory = 1290755856 SRR26075348 SRAfilesize 05c71f9b2dd09653b7df202d7abdf416 SRR26075348.sra SRR26075348.sra file validated SRR26075348 is paired end SRR26075348 is conventional basespace SRR26075348 read1 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR26075348_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.568 37.0 37.0 37.0 37.0 37.0 2 36.6635 37.0 37.0 37.0 37.0 37.0 3 36.684 37.0 37.0 37.0 37.0 37.0 4 36.632 37.0 37.0 37.0 37.0 37.0 5 36.6745 37.0 37.0 37.0 37.0 37.0 6 36.6785 37.0 37.0 37.0 37.0 37.0 7 36.593 37.0 37.0 37.0 37.0 37.0 8 36.654 37.0 37.0 37.0 37.0 37.0 9 36.6805 37.0 37.0 37.0 37.0 37.0 10-14 36.638600000000004 37.0 37.0 37.0 37.0 37.0 15-19 36.636 37.0 37.0 37.0 37.0 37.0 20-24 36.5733 37.0 37.0 37.0 37.0 37.0 25-29 36.4603 37.0 37.0 37.0 37.0 37.0 30-34 36.4387 37.0 37.0 37.0 37.0 37.0 35-39 36.4134 37.0 37.0 37.0 37.0 37.0 40-44 36.35979999999999 37.0 37.0 37.0 37.0 37.0 45-49 36.3215 37.0 37.0 37.0 37.0 37.0 50-54 36.31320000000001 37.0 37.0 37.0 37.0 37.0 55-59 36.259299999999996 37.0 37.0 37.0 37.0 37.0 60-64 36.20270000000001 37.0 37.0 37.0 37.0 37.0 65-69 36.1754 37.0 37.0 37.0 37.0 37.0 70-74 36.1608 37.0 37.0 37.0 37.0 37.0 75-79 36.1055 37.0 37.0 37.0 37.0 37.0 80-84 36.05219999999999 37.0 37.0 37.0 37.0 37.0 85-89 35.9088 37.0 37.0 37.0 37.0 37.0 90-94 35.948899999999995 37.0 37.0 37.0 37.0 37.0 95-99 35.882799999999996 37.0 37.0 37.0 37.0 37.0 100-104 35.9269 37.0 37.0 37.0 37.0 37.0 105-109 35.8254 37.0 37.0 37.0 37.0 37.0 110-114 35.7616 37.0 37.0 37.0 37.0 37.0 115-119 35.6126 37.0 37.0 37.0 37.0 37.0 120-124 35.627199999999995 37.0 37.0 37.0 37.0 37.0 125-129 35.4307 37.0 37.0 37.0 37.0 37.0 130-134 35.36800000000001 37.0 37.0 37.0 34.6 37.0 135-139 35.2844 37.0 37.0 37.0 34.6 37.0 140-144 35.1145 37.0 37.0 37.0 27.4 37.0 145-149 35.0589 37.0 37.0 37.0 25.0 37.0 150-151 34.99875 37.0 37.0 37.0 25.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.0 1101 2 0.0 1101 3 0.0 1101 4 0.0 1101 5 0.0 1101 6 0.0 1101 7 0.0 1101 8 0.0 1101 9 0.0 1101 10-14 0.0 1101 15-19 0.0 1101 20-24 0.0 1101 25-29 0.0 1101 30-34 0.0 1101 35-39 0.0 1101 40-44 0.0 1101 45-49 0.0 1101 50-54 0.0 1101 55-59 0.0 1101 60-64 0.0 1101 65-69 0.0 1101 70-74 0.0 1101 75-79 0.0 1101 80-84 0.0 1101 85-89 0.0 1101 90-94 0.0 1101 95-99 0.0 1101 100-104 0.0 1101 105-109 0.0 1101 110-114 0.0 1101 115-119 0.0 1101 120-124 0.0 1101 125-129 0.0 1101 130-134 0.0 1101 135-139 0.0 1101 140-144 0.0 1101 145-149 0.0 1101 150-151 0.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 18 1.0 19 0.0 20 2.0 21 1.0 22 5.0 23 1.0 24 7.0 25 9.0 26 9.0 27 9.0 28 22.0 29 22.0 30 28.0 31 30.0 32 68.0 33 90.0 34 155.0 35 401.0 36 2941.0 37 199.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 46.238716148445334 13.139418254764292 7.923771313941826 32.698094282848544 2 20.05 12.525 32.775 34.65 3 16.475 19.075 30.7 33.75 4 22.675 25.174999999999997 25.2 26.950000000000003 5 24.15 30.4 24.349999999999998 21.099999999999998 6 22.675 32.15 23.825 21.349999999999998 7 15.049999999999999 27.275 42.175000000000004 15.5 8 18.65 26.025 31.900000000000002 23.425 9 18.25 24.025 35.75 21.975 10-14 20.915 29.94 26.36 22.785 15-19 19.97 27.860000000000003 28.12 24.05 20-24 20.69 27.51 28.09 23.71 25-29 20.244999999999997 27.88 27.93 23.945 30-34 19.99 28.185 27.47 24.355 35-39 20.59 27.315 27.85 24.245 40-44 21.09 28.199999999999996 27.584999999999997 23.125 45-49 20.06 28.475 27.74 23.724999999999998 50-54 20.465 27.705000000000002 27.93 23.9 55-59 19.665 28.48 27.694999999999997 24.16 60-64 20.335 26.889999999999997 28.46 24.315 65-69 20.5 28.13 26.91 24.46 70-74 20.03 28.34 27.465 24.165 75-79 20.22 27.515 27.505000000000003 24.759999999999998 80-84 20.8 28.585 26.43 24.185000000000002 85-89 20.985 27.58 27.860000000000003 23.575 90-94 21.265 27.650000000000002 27.650000000000002 23.435 95-99 21.12 26.790000000000003 27.939999999999998 24.15 100-104 21.04 27.87 27.42 23.669999999999998 105-109 21.095 27.41 27.800000000000004 23.695 110-114 21.175 27.865000000000002 26.855 24.104999999999997 115-119 21.745 27.889999999999997 27.67 22.695 120-124 20.94 27.500000000000004 27.58 23.98 125-129 21.349999999999998 27.950000000000003 26.77 23.93 130-134 21.81 28.51 26.19 23.49 135-139 21.545 27.439999999999998 26.75 24.265 140-144 21.535 26.965 27.345000000000002 24.154999999999998 145-149 22.09 27.229999999999997 26.200000000000003 24.48 150-151 21.8625 26.35 26.6625 25.124999999999996 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.5 7 0.5 8 0.0 9 0.0 10 1.0 11 1.0 12 0.0 13 0.5 14 0.5 15 0.0 16 1.0 17 1.0 18 0.0 19 0.0 20 0.0 21 0.5 22 2.5 23 2.5 24 2.5 25 5.0 26 4.0 27 2.5 28 7.5 29 14.5 30 14.5 31 13.5 32 25.5 33 30.5 34 35.5 35 58.5 36 86.0 37 109.0 38 117.0 39 131.0 40 166.0 41 216.5 42 231.0 43 237.5 44 255.0 45 254.5 46 269.0 47 294.0 48 272.0 49 227.0 50 185.5 51 137.0 52 107.0 53 97.5 54 82.5 55 59.0 56 53.5 57 43.5 58 34.0 59 23.0 60 11.5 61 12.0 62 17.0 63 16.5 64 7.5 65 4.0 66 6.0 67 3.5 68 2.5 69 3.0 70 2.0 71 1.0 72 0.0 73 0.0 74 0.0 75 0.0 76 0.0 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150-151 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 62.050000000000004 #Duplication Level Percentage of deduplicated Percentage of total 1 64.46414182111201 40.0 2 20.30620467365028 25.2 3 9.105560032232072 16.950000000000003 4 3.585817888799355 8.9 5 1.4907332796132151 4.625 6 0.6446414182111201 2.4 7 0.201450443190975 0.8750000000000001 8 0.12087026591458502 0.6 9 0.08058017727639001 0.44999999999999996 >10 0.0 0.0 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CCCGTCTCAGCAACTGAACAAGAGGCAACATGTAAGCCAGAGTCTTTCCA 9 0.22499999999999998 No Hit GCTTCTATTGCAAATATTGCATCAAACGTGTTGTCAGAAAATGGCATTTT 9 0.22499999999999998 No Hit ATAGAGCCCGCAGTCTCCCAGTCACCTGCATGTATATCAACTCCTTGGAT 8 0.2 No Hit ACGTATAGAGAATGGTAATGCATATACCAAGTGCGAAAGAATGTGCATTT 8 0.2 No Hit GCCCTTTCTTGAATTTCAACTTCTTCACCAGAGGTATTACGGATCTCGGG 8 0.2 No Hit GTGTGAAAAGAATGCCATTCAGTCGGACAAACCGAACACAGAAGGACTTG 7 0.17500000000000002 No Hit GCTTGTGTAGAGCACGTGAGAGCTGCACCTGCTACCTGTTATTCTCTCAC 7 0.17500000000000002 No Hit GTCAGATATACTTGCATGTTACAACAAGGTCTCCAAAAACTCGCTGATTG 7 0.17500000000000002 No Hit ACCCACTCTCCCAAAATGACACTTTTTTCTCCACTTCTTCGTCAACTTCC 7 0.17500000000000002 No Hit GCCAGTAGCAGCAACCCTGAGGACAGATTCAGCAGCTCCAATGGTATTAG 7 0.17500000000000002 No Hit GTTACCAAGTGTAGATTTTGGAGCCACTACCATATGAGGACCAGTGATTC 6 0.15 No Hit CTTCTGGACTACCTTGAGAGATGCCTTGTTGTCGTTGTTGCTGAGAACTT 6 0.15 No Hit CTCATTACCATCAGCAATGCACCTCTCATCTCGTCTCTTGATAGGACTGT 6 0.15 No Hit CCCAAAAGTGCAAGATCCTTTGTTGAAGTTCTCACAGATCTTGGTCTTGA 6 0.15 No Hit CTACTATTTCTCTCCTCCTTCACCACGGACACCACTAGCACGGCCACCCC 6 0.15 No Hit CTCGTATATTTTCTCTAGTCTCTGAAGACGATATATACATGGACGGACAT 6 0.15 No Hit GGCTAGGCTGCTGGCCAGTAGAATAACCTTGCTGTCCTGTTGCAGATGGT 6 0.15 No Hit GCTCATATGGATGCCGCGGATCTTTGAGCATACCTCGGCAGAAATTCAGC 6 0.15 No Hit GTCGGACTGGTGAGACTCCACTTCTTGCCATACCAAAATTGTTGCATACC 6 0.15 No Hit CACCGCCTTGTCCTAGGTCAGGAGTCATGGGGTGCATAGCATCACCAGCT 6 0.15 No Hit AGTTCCTCCACCGAGTGAGTGGCAAACTTGAAATCCTTGAAGACAGTCAC 6 0.15 No Hit GCCTAGTCAACCAGATGTGTGCTAGCCCGTCGAGACTGAAAAGCTATAAC 6 0.15 No Hit CTCAGCTTTTGCATCTGATTCTGAAGGATTCATACGAATGTATAGTTCCT 6 0.15 No Hit GCTGCGCGTGACGAACCACTGAGTGGCCGTTATAACGTTGCTGTTCGCCC 6 0.15 No Hit CGACAAATCCAGCATGGGCAGGTTCATGCCCACTCCCTCCTCCTGATATA 6 0.15 No Hit CTTGATTGTTGATCTGCTGCTGAGCAGCTACAGTCCTAAGGACCTCCATA 6 0.15 No Hit GCTGTGGTTCCAAATCTTCTAGCAACCTCGGCCTCAGACAATGTATCCCA 5 0.125 No Hit GCCACAGCTAGCATAGATGCCGAACAATCCAGTTTTATTGAAGATGCTGT 5 0.125 No Hit CAAAAGTCATAGGCCAAATAGATCCAGTGCGAGCCCAGTTCATCAAACTA 5 0.125 No Hit CATAGTTCCTCTGCTAGCTAGTAGTTTATCAAATAACTAGTAAGGACCCC 5 0.125 No Hit TCTCACTACACCGCTGTTACCATGGGGCCTAGTGACCTTGCCCCAAATGC 5 0.125 No Hit CAATGGAATAAATCTCTAAATTGATATCACCTAACACAGCCTAACAGAGG 5 0.125 No Hit GCCAAATCTGAAGCATCCAATGGGAGTCCAGCAATACCTTTTCCCGAAGT 5 0.125 No Hit GTCCAAGTGAGCCATGTTCCATATATTCGTAGATGAGCAAAATTAGGTGC 5 0.125 No Hit TATGGCTATCAGGGAGTGTAGGATGCAGATCGTAGAGAAAGCTGACGAAG 5 0.125 No Hit CCTGTCCAGTTGTAAACGATGGCCCCGGCAGGCAAAGAAATGCGACAACA 5 0.125 No Hit CTCTGCTAAACTTGAGTTCTGTTTCATACTGCTTCTTCTGCAACCTAAAA 5 0.125 No Hit GCACAGAAGGTTCCTACATGAGTTTGCTATAGCAGATATTGCAGCATCGG 5 0.125 No Hit ATGACGATTCTCATCCTCAGGGGCACCATGTTCCTTGCCTACAGGATTAA 5 0.125 No Hit GGCGCCCCTTGAATGAAACTCAAGATTCGAGTGGGGCTCGGCTTATGGCC 5 0.125 No Hit GTCTGATTCTGGATAACATGTGAACCCTGGCGCCAGGCCTTAGGTTTCTG 5 0.125 No Hit GTTTGGAAAGCGTTTGAGCAGACCGACCTCAAGGTGTCCGAACCAACAAC 5 0.125 No Hit ACAACGCTCCTGGATTTCTGAAGTGCCAACTCCAATAAACTGGCTAACAA 5 0.125 No Hit ACGAGACCGAGAGTGAATCTTGGTTGGCTTCCTGATAATGAAGCCATCCT 5 0.125 No Hit CCCCTATCCAGAACCTTCTCAATGTTCTCCATCATGACACCTTTAACTTC 5 0.125 No Hit GCAAACTAGAGGGATTTTCCTTTGATGTAGCCAGAGGAGGAACATTCCTT 5 0.125 No Hit AGACAGTTGCACCTGCTGATCCAGTTCCCTGTAGATGTAGATGTTCCAGT 5 0.125 No Hit CTCTTATCAAAATTATAATTTCCTATCACAGCACTACGGTGTAGAGTGCT 5 0.125 No Hit CCTCACCAATTCCTCAAAAAACCTCCTCTATTATCAATTCGGTCAATTGA 5 0.125 No Hit CCAGGAGGTAGTAAAACCATACAGAGTGCTGCAGAGGCAAGAACAGTTGC 5 0.125 No Hit GTCAGACAAAAACAAAGCTTAGTCCATAAGGTCCCCAATGGAGTCGTCAT 5 0.125 No Hit TCATCATCATAAAAAGCAAGCATCCGATGGGAAATGGAACACAGTTCTGA 5 0.125 No Hit CCAGCATACACAAGCAACTTGATTCCATCCTCAAGGAGAGCAGGAATGCC 5 0.125 No Hit GGGTTCTTCACATGCCTTCCTACTTGTTTTTCTTATCCTCATTTAGCTGT 5 0.125 No Hit GTCACCATACCTACGAGACCCATCTGAAATTGTTTTCTTCTGGGATGTTT 5 0.125 No Hit ATCCTGTCTAAAATCTCATCCAAGACTATCCTGCCACTGCTGGCCAGATA 5 0.125 No Hit GGTAAAACTCCCCTGCTAATCTTCGACAACACCATTGCCACCATTCTCAG 5 0.125 No Hit AGTAGCCTGAATCTCATAGTGAATACCATCCAGCTCCCTTCTAAATCTGT 5 0.125 No Hit CTCACGAATTACAACCCAGAACAGTTACATCAGTGCACTAGCACCACCAC 5 0.125 No Hit CCAATCTTTTGTTTCTTCCGGCGATAGAGGAAAATGATCAGAAATCCAGC 5 0.125 No Hit CTCTTGTCCTTCAATGGCAATGCCAACCAAGGCATTGTCTCAAAACTCTC 5 0.125 No Hit GGGTCAACCATGGATATGGAAAAGCATTCAAGACCTTCTTGTTGTATATG 5 0.125 No Hit GTCATGGTTAATACCAAAGGATGATATAGATGCAACAAAAAGTGCTGCAC 5 0.125 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0 0.0 0.0 0.0 0.0 64-65 0.0 0.0 0.0 0.0 0.0 66-67 0.0 0.0 0.0 0.0 0.0 68-69 0.0 0.0 0.0 0.0 0.0 70-71 0.0 0.0 0.0 0.0 0.0 72-73 0.0 0.0 0.0 0.0 0.0 74-75 0.0 0.0 0.0 0.0 0.0 76-77 0.125 0.0 0.0 0.0 0.0 78-79 0.15 0.0 0.0 0.0 0.0 80-81 0.1875 0.0 0.0 0.0 0.0 82-83 0.2 0.0 0.0 0.0 0.0 84-85 0.21250000000000002 0.0 0.0 0.0 0.0 86-87 0.35 0.0 0.0 0.0 0.0 88-89 0.45 0.0 0.0 0.0 0.0 90-91 0.575 0.0 0.0 0.0 0.0 92-93 0.625 0.0 0.0 0.0 0.0 94-95 0.825 0.0 0.0 0.0 0.0 96-97 1.175 0.0 0.0 0.0 0.0 98-99 1.4 0.0 0.0 0.0 0.0 100-101 1.5875 0.0 0.0 0.0 0.0 102-103 1.7625000000000002 0.0 0.0 0.0 0.0 104-105 2.0625 0.0 0.0 0.0 0.0 106-107 2.2625 0.0 0.0 0.0 0.0 108-109 2.5625 0.0 0.0 0.0 0.0 110-111 2.7875 0.0 0.0 0.0 0.0 112-113 3.0125 0.0 0.0 0.0 0.0 114-115 3.5999999999999996 0.0 0.0 0.0 0.0 116-117 4.0875 0.0 0.0 0.0 0.0 118-119 4.7625 0.0 0.0 0.0 0.0 120-121 5.3375 0.0 0.0 0.0 0.0 122-123 5.762499999999999 0.0 0.0 0.0 0.0 124-125 6.325 0.0 0.0 0.0 0.0 126-127 6.824999999999999 0.0 0.0 0.0 0.0 128-129 7.25 0.0 0.0 0.0 0.0 130-131 8.05 0.0 0.0 0.0 0.0 132-133 8.7875 0.0 0.0 0.0 0.0 134-135 9.45 0.0 0.0 0.0 0.0 136-137 10.1625 0.0 0.0 0.0 0.0 138-139 10.9875 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CCAAATC 10 0.006830828 145.0 2 CTCTTGT 10 0.006830828 145.0 1 CTGAAGC 10 0.006830828 145.0 8 ATAAAAG 10 0.006830828 145.0 9 CCTTCAA 10 0.006830828 145.0 8 TCAAACA 20 3.5877043E-4 108.75 3 AAAAAAA 175 9.405505E-5 9.114285 25-29 >>END_MODULE SRR26075348 read2 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR26075348_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.17475 37.0 37.0 37.0 37.0 37.0 2 36.3475 37.0 37.0 37.0 37.0 37.0 3 36.4055 37.0 37.0 37.0 37.0 37.0 4 36.31 37.0 37.0 37.0 37.0 37.0 5 36.4235 37.0 37.0 37.0 37.0 37.0 6 36.3275 37.0 37.0 37.0 37.0 37.0 7 36.261 37.0 37.0 37.0 37.0 37.0 8 36.276 37.0 37.0 37.0 37.0 37.0 9 36.3045 37.0 37.0 37.0 37.0 37.0 10-14 36.2821 37.0 37.0 37.0 37.0 37.0 15-19 36.2676 37.0 37.0 37.0 37.0 37.0 20-24 36.220600000000005 37.0 37.0 37.0 37.0 37.0 25-29 36.160000000000004 37.0 37.0 37.0 37.0 37.0 30-34 36.0656 37.0 37.0 37.0 37.0 37.0 35-39 36.0409 37.0 37.0 37.0 37.0 37.0 40-44 35.9961 37.0 37.0 37.0 37.0 37.0 45-49 35.9362 37.0 37.0 37.0 37.0 37.0 50-54 35.8454 37.0 37.0 37.0 37.0 37.0 55-59 35.839999999999996 37.0 37.0 37.0 37.0 37.0 60-64 35.9307 37.0 37.0 37.0 37.0 37.0 65-69 35.8421 37.0 37.0 37.0 37.0 37.0 70-74 35.691700000000004 37.0 37.0 37.0 37.0 37.0 75-79 35.63349999999999 37.0 37.0 37.0 37.0 37.0 80-84 35.7159 37.0 37.0 37.0 37.0 37.0 85-89 35.653499999999994 37.0 37.0 37.0 37.0 37.0 90-94 35.514599999999994 37.0 37.0 37.0 37.0 37.0 95-99 35.589 37.0 37.0 37.0 37.0 37.0 100-104 35.5435 37.0 37.0 37.0 37.0 37.0 105-109 35.3707 37.0 37.0 37.0 37.0 37.0 110-114 35.327799999999996 37.0 37.0 37.0 34.6 37.0 115-119 35.358 37.0 37.0 37.0 37.0 37.0 120-124 35.20685 37.0 37.0 37.0 32.2 37.0 125-129 35.309799999999996 37.0 37.0 37.0 37.0 37.0 130-134 35.142399999999995 37.0 37.0 37.0 29.8 37.0 135-139 34.9611 37.0 37.0 37.0 25.0 37.0 140-144 34.93655 37.0 37.0 37.0 25.0 37.0 145-149 34.89535 37.0 37.0 37.0 25.0 37.0 150-151 34.589124999999996 37.0 37.0 37.0 25.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.0 1101 2 0.0 1101 3 0.0 1101 4 0.0 1101 5 0.0 1101 6 0.0 1101 7 0.0 1101 8 0.0 1101 9 0.0 1101 10-14 0.0 1101 15-19 0.0 1101 20-24 0.0 1101 25-29 0.0 1101 30-34 0.0 1101 35-39 0.0 1101 40-44 0.0 1101 45-49 0.0 1101 50-54 0.0 1101 55-59 0.0 1101 60-64 0.0 1101 65-69 0.0 1101 70-74 0.0 1101 75-79 0.0 1101 80-84 0.0 1101 85-89 0.0 1101 90-94 0.0 1101 95-99 0.0 1101 100-104 0.0 1101 105-109 0.0 1101 110-114 0.0 1101 115-119 0.0 1101 120-124 0.0 1101 125-129 0.0 1101 130-134 0.0 1101 135-139 0.0 1101 140-144 0.0 1101 145-149 0.0 1101 150-151 0.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 13 4.0 14 5.0 15 6.0 16 3.0 17 3.0 18 5.0 19 6.0 20 3.0 21 6.0 22 3.0 23 7.0 24 12.0 25 11.0 26 11.0 27 13.0 28 21.0 29 18.0 30 26.0 31 40.0 32 57.0 33 98.0 34 200.0 35 627.0 36 2615.0 37 200.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 46.73668417104276 23.380845211302827 9.802450612653162 20.080020005001252 2 28.975 25.174999999999997 28.175 17.675 3 19.950000000000003 28.549999999999997 32.65 18.85 4 24.55 33.4 23.225 18.825 5 25.624999999999996 37.4 19.925 17.05 6 22.55 36.075 22.75 18.625 7 22.775000000000002 22.7 34.775 19.75 8 22.075 26.325 27.6 24.0 9 23.525 24.45 28.925 23.1 10-14 24.36 29.29 26.090000000000003 20.26 15-19 23.505000000000003 28.835 26.515 21.145 20-24 23.685000000000002 28.825 26.700000000000003 20.79 25-29 23.91 28.854999999999997 25.96 21.275 30-34 23.474999999999998 27.805000000000003 27.605 21.115000000000002 35-39 24.57 27.265 27.089999999999996 21.075 40-44 24.21 28.775000000000002 26.529999999999998 20.485 45-49 23.799999999999997 29.054999999999996 26.229999999999997 20.915 50-54 23.044999999999998 28.084999999999997 27.97 20.9 55-59 23.73 27.415 27.67 21.185000000000002 60-64 23.765 27.634999999999998 27.655 20.945 65-69 24.02 27.415 27.575 20.990000000000002 70-74 23.880000000000003 27.655 26.655 21.81 75-79 23.98 29.220000000000002 26.009999999999998 20.79 80-84 24.72 28.71 26.090000000000003 20.48 85-89 24.585 27.534999999999997 27.805000000000003 20.075000000000003 90-94 23.61 28.265 27.045 21.08 95-99 23.94 27.900000000000002 27.725 20.435 100-104 24.125 28.499999999999996 26.19 21.185000000000002 105-109 24.675 27.839999999999996 26.63 20.855 110-114 25.264999999999997 27.994999999999997 27.275 19.465 115-119 25.055 28.299999999999997 26.875 19.77 120-124 24.911245562278115 28.97144857242862 26.206310315515775 19.91099554977749 125-129 25.014999999999997 29.65 25.185000000000002 20.150000000000002 130-134 26.005 28.535 25.430000000000003 20.03 135-139 25.917591759175917 27.787778777877786 26.237623762376238 20.05700570057006 140-144 26.498974846226936 28.949342401360205 25.678851827774167 18.872830924638695 145-149 25.936484121030258 28.877219304826205 25.696424106026505 19.489872468117028 150-151 28.178522315289413 29.428678584823103 24.32804100512564 18.064758094761846 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 0.0 1 0.0 2 0.5 3 0.5 4 0.0 5 0.0 6 0.0 7 0.5 8 0.5 9 0.0 10 1.0 11 1.5 12 1.0 13 1.0 14 0.5 15 0.5 16 1.0 17 1.0 18 0.5 19 1.5 20 1.5 21 1.5 22 2.0 23 0.5 24 1.0 25 2.5 26 2.0 27 1.0 28 3.5 29 6.0 30 7.5 31 10.5 32 13.0 33 17.0 34 31.5 35 55.5 36 67.0 37 85.5 38 111.0 39 141.5 40 208.5 41 244.5 42 261.5 43 275.0 44 290.0 45 289.5 46 275.5 47 268.0 48 232.0 49 215.0 50 192.0 51 153.5 52 119.5 53 86.0 54 64.5 55 48.0 56 38.0 57 30.0 58 15.5 59 14.0 60 19.5 61 15.0 62 14.0 63 10.0 64 3.5 65 6.0 66 5.5 67 2.5 68 1.0 69 2.5 70 3.0 71 1.0 72 0.5 73 0.5 74 0.0 75 0.5 76 0.5 77 0.0 78 0.5 79 0.5 80 0.0 81 0.0 82 0.5 83 0.5 84 0.5 85 1.0 86 1.0 87 0.5 88 0.5 89 1.0 90 1.0 91 1.0 92 1.0 93 0.5 94 0.0 95 0.0 96 0.0 97 0.5 98 0.5 99 1.0 100 5.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.025 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.005 125-129 0.0 130-134 0.0 135-139 0.01 140-144 0.015 145-149 0.025 150-151 0.0125 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 62.150000000000006 #Duplication Level Percentage of deduplicated Percentage of total 1 65.36604987932422 40.625 2 19.428801287208366 24.15 3 8.809332260659694 16.425 4 3.8616251005631534 9.6 5 1.3676588897827837 4.25 6 0.7240547063555913 2.7 7 0.2413515687851971 1.05 8 0.12067578439259855 0.6 9 0.04022526146419952 0.22499999999999998 >10 0.04022526146419952 0.375 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG 15 0.375 No Hit ATTGAGGTTCCTACTGAGATTCAATGTATTGGTATTCCTGCTATTTTGGA 9 0.22499999999999998 No Hit GGGTGTGGAGCAGGAGTTCATTTCTAAGAATTCTCTCAGTGAAGAAGTTT 8 0.2 No Hit TTTGTGTTGATATAGAAAACAATGGCACTACATGGAAAGATTGAGACAAC 8 0.2 No Hit GGGAGGGATGTGGGCCTTAACCAGATATCTATGTTCGAGGCAAAGATAGC 8 0.2 No Hit GTTGCATTTATCTAAAGTATTGTCACTTTACTTAACACTTGAGCTGCATA 7 0.17500000000000002 No Hit CGGGATTCTAGCCTCTTCTCTTTGATGTCTGATGTCCTCATCGTCCTCCT 7 0.17500000000000002 No Hit GGGAAGAAGCTTGAGAAGATTTATGTACCAGAAGATGAGCTGCTTATGAA 7 0.17500000000000002 No Hit ATTTGTTCGACACTTCAATTGCTTTATGCCCTCCACATCTGCTTTTGTCT 7 0.17500000000000002 No Hit TTCACCTGTGCGCTTGACTGGCAAAGGTAGAGGGAAGAGAGAAGCAAACC 7 0.17500000000000002 No Hit ATTTCGTTGGCAGCATCATCTTCTGGTCCATTTTCTTCCATTTCTTCACA 7 0.17500000000000002 No Hit GTCTAAGGGCGGTGTACACCCTTTTGAGCAATGATTGCACAACCTGCGAT 6 0.15 No Hit ATTTTCAGTACTGCTTGCTGCACTAAAAATCCTCATTTTGATATCAAAAC 6 0.15 No Hit AGACATTGCAGGGATGGGGTCTGACCTATTTGGATCTTATGCTGAATCAT 6 0.15 No Hit GCACAACCCATGCCCATTACTGCTTTAGCTACTGCCCTGGCAAATGCAAC 6 0.15 No Hit AGAACCTTGTTATCATTCGTGATCAAGCTATTTGCACTTTGTCGTAGTCT 6 0.15 No Hit GTCGATATCATATTTAAAACTGGATGGTCTTGTAAGGAGAAGAGCCCAGA 6 0.15 No Hit AAACAAGAGATTGATGAGATTGTTGGGATTGGTTATTTGAATGGGCTCTT 6 0.15 No Hit GCCACAGGCGTCATACTTCCCAAGAAGCGGCCATAGCCCAGATGCGAGGT 6 0.15 No Hit GGAGAACATAATTGAGAAGGCAAAATATGGCCACTCCCATTTCCTCCGTC 6 0.15 No Hit ATTGAACTGCCAGAAGGGAGATGGATTCGTGAAATATCTGGGGCTGAAAT 6 0.15 No Hit CATGGCCCCTTTGAAACTCCATGGAAGCGTTTTATCTACCAACACACAGC 6 0.15 No Hit ACCAACAAGAGCACAGGGGCGACGCACAAGGTGAGCGCAAGGAAGGGTTT 6 0.15 No Hit GCCAGATCTTTAGGCCTGATAACTTTGTGTTTGGACAGTCTGGCGCTGGA 6 0.15 No Hit GACTGTATGAGAATGGTATAAACGGAATTCTTGCAGATGAAATGGGTCTT 6 0.15 No Hit CGAGAAAATACTTGAGAGAGGTGACCGAATTGAGCTTCTTGTTGACAAAA 6 0.15 No Hit CTTATGATCAACAGCAAGGTTATACTGCTGCTCCTAACTATAGCAATGTA 6 0.15 No Hit ATTTGATCAGATCAGTCAAGCAAGTGACATGGTTCGCCAGCTTATTTCAA 6 0.15 No Hit CTTCAACATACCTAGACGTGGTCCGCCATGCTGATCTTTCAACATTAACA 6 0.15 No Hit ATTTATTTGTGGTGAGTCTTGAAGATGGTACAGTTCAAGGTTTTGACATC 5 0.125 No Hit GAAATAGGAAGTGAAGTTTATGCCAGGAAGGCCTTGGACTTGAAACATGA 5 0.125 No Hit GGACCTTGAAGGAAAATTGGTTGGCTTGTATTTTTCAGCCCATGCTCATA 5 0.125 No Hit GTCACGGTAAGGGTATTTCAGCTTCAGCTTTGCCTTACAAGAGAACCCCA 5 0.125 No Hit CATCACTCAAAGCAATGAAACCTGTTGGCACTAAGCCAATCGAGCCTGTC 5 0.125 No Hit CCCAAAACCAGAAGCCACACAGGGGTCCTTTGTTCCCTGTTGAAGCCGCC 5 0.125 No Hit GAGCAGGCTTCTCTTCAGCTTATACTGGAGGATAAAGATTTGCAACTCTT 5 0.125 No Hit GGCTGCATGTCAACTGGGCCGCATTTTAATCCTGTAGGCAAGGAACATGG 5 0.125 No Hit ATGTGTTCTAGGCAGAAATAGACTCCTATCTCGCTCAGTTTCACAACAAG 5 0.125 No Hit GTTATTATCATCAACTTTACTTTCATTCTTGCTAATATGATTACCCCCCA 5 0.125 No Hit CAACAGCTCTCGAAGAAGACGCGGAGATGGTTTCCCTCAAACTCCAAAAG 5 0.125 No Hit TGACAATAAGGGCCTTTGAGGAGGAAGAACGTTTCTTCGCAAAAACCCTT 5 0.125 No Hit ACACCAGCTCCCTACACTCGCGCACCGCCACCTTCCTCATCCTCACTAAC 5 0.125 No Hit GTTACTGGTAACCTTGACTCTAGTACCATACAACAAATGATGATTCCACG 5 0.125 No Hit GAAGATTTCACTGCATCTTTGCCTGAGAACGACTGTCGATACGCTGTTTA 5 0.125 No Hit AGAAAGGGTAAAGAAAGATGATTTCAGCAAGATCGTTCCATCAGAATGCC 5 0.125 No Hit GGAATTTGGGCCAAAATTGAAGGAACATATGAAGTATTGTGCTGATCATC 5 0.125 No Hit GCTGGAAGGATTGTCTTTGTGTCCTCTATTTGCGGCGTGACATCTGTCAA 5 0.125 No Hit ATGTTGCTCTAGCTTTTGAAGCCAGTGGGGGATGGCATAAAGAGAAAGAT 5 0.125 No Hit CAAAATACTAGGTGGCGACTCCCATTCCATTTTTCTACTAATAAAAGACA 5 0.125 No Hit GCTTGCTCCACCAGTGCTACCAAAGTTTGAAGATCCTGTTGCAGCTGCAC 5 0.125 No Hit GCAGAAGAGGAAGTCAAGCCTGTGAAAAGAAAGTGCAAATCTAAGAAGAT 5 0.125 No Hit GAAAGTCTACAGCTCTTTTCTGGTGGCACTTCGTCGTTGCAAGATGCAAG 5 0.125 No Hit GAAATGGTGAAGGGACGCCAAGGAGAGCGAGTCAGGCTTTATGTACGGGG 5 0.125 No Hit GAAATTCTTCAGGCACATTGCAACCTAACCCACTGTTCAAATTCATCAAA 5 0.125 No Hit CCCAGCGGTGTGTATCGTTATTATTGTGGCAAAGGATTTGAGTGGGAAAT 5 0.125 No Hit GACCAGTCAGTGGTTGGCAGGAGACGCATATACTATGATCGACATGGCAG 5 0.125 No Hit CTCTCGCGCAAAATACTTCCCAGAAAGTGGATATTTTCCCGGAAAATCGA 5 0.125 No Hit GCAATTGCAGCAATATGCAACTTGTGTGCTGGAGATGGGATTGCAGATAT 5 0.125 No Hit CTATACTCCGATTGTTTTCATTCAAACAAGTGTAATGTTGCTGGCGAGCT 5 0.125 No Hit GTGCTCGGTTGTGTTCACAGGAAGTATTTTCCAAGCGGATTGCTGAGATG 5 0.125 No Hit GTCTATCTGTATGCTCAATTGATTATAGTTTTCTGTCAGAGTTTGAAGCT 5 0.125 No Hit GACATAGTGATCTTCTGCATATTGATGCAGGTCACTGCTTTTCAGAAGTT 5 0.125 No Hit GGACAGGCCTTGGGTGGTCACAAGAGGTGTCACTATGAAGGCATCATAGG 5 0.125 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0 0.0 0.0 0.0 0.0 64-65 0.0 0.0 0.0 0.0 0.0 66-67 0.0 0.0 0.0 0.0 0.0 68-69 0.0 0.0 0.0 0.0 0.0 70-71 0.0 0.0 0.0 0.0 0.0 72-73 0.0 0.0 0.0 0.0 0.0 74-75 0.0 0.0 0.0 0.0 0.0 76-77 0.125 0.0 0.0 0.0 0.0 78-79 0.15 0.0 0.0 0.0 0.0 80-81 0.1875 0.0 0.0 0.0 0.0 82-83 0.2 0.0 0.0 0.0 0.0 84-85 0.21250000000000002 0.0 0.0 0.0 0.0 86-87 0.35 0.0 0.0 0.0 0.0 88-89 0.45 0.0 0.0 0.0 0.0 90-91 0.575 0.0 0.0 0.0 0.0 92-93 0.625 0.0 0.0 0.0 0.0 94-95 0.825 0.0 0.0 0.0 0.0 96-97 1.175 0.0 0.0 0.0 0.0 98-99 1.4125 0.0 0.0 0.0 0.0 100-101 1.6125 0.0 0.0 0.0 0.0 102-103 1.7875 0.0 0.0 0.0 0.0 104-105 2.1125 0.0 0.0 0.0 0.0 106-107 2.3125 0.0 0.0 0.0 0.0 108-109 2.6125 0.0 0.0 0.0 0.0 110-111 2.8375 0.0 0.0 0.0 0.0 112-113 3.0625 0.0 0.0 0.0 0.0 114-115 3.675 0.0 0.0 0.0 0.0 116-117 4.1625 0.0 0.0 0.0 0.0 118-119 4.8625 0.0 0.0 0.0 0.0 120-121 5.425000000000001 0.0 0.0 0.0 0.0 122-123 5.862500000000001 0.0 0.0 0.0 0.0 124-125 6.4 0.0 0.0 0.0 0.0 126-127 6.9 0.0 0.0 0.0 0.0 128-129 7.35 0.0 0.0 0.0 0.0 130-131 8.1375 0.0 0.0 0.0 0.0 132-133 8.899999999999999 0.0 0.0 0.0 0.0 134-135 9.55 0.0 0.0 0.0 0.0 136-137 10.2375 0.0 0.0 0.0 0.0 138-139 11.1375 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GAAATTT 10 0.006830828 145.0 9 GGACCTT 10 0.006830828 145.0 1 TAAAAAC 10 0.006830828 145.0 1 CCTTGAA 10 0.006830828 145.0 4 GGATAAT 10 0.006830828 145.0 2 ACATAGA 10 0.006830828 145.0 6 ATAGAAG 10 0.006830828 145.0 8 CATAGAA 10 0.006830828 145.0 7 GGGATAA 10 0.006830828 145.0 1 CGACGAA 10 0.006830828 145.0 145 AAAACAT 10 0.006830828 145.0 3 GACCTTG 10 0.006830828 145.0 2 GAAAGCA 10 0.006830828 145.0 9 AACATAG 10 0.006830828 145.0 5 GGAGATC 40 0.0076550315 18.125 135-139 >>END_MODULE Read 2477440 spots for SRR26075348.sra Written 2477440 spots for SRR26075348.sra Read 2477440 spots for SRR26075348.sra Written 2477440 spots for SRR26075348.sra Read 2477440 spots for SRR26075348.sra Written 2477440 spots for SRR26075348.sra Read 2477440 spots for SRR26075348.sra Written 2477440 spots for SRR26075348.sra Read 2477447 spots for SRR26075348.sra Written 2477447 spots for SRR26075348.sra Read 2477440 spots for SRR26075348.sra Written 2477440 spots for SRR26075348.sra Read 2477440 spots for SRR26075348.sra Written 2477440 spots for SRR26075348.sra Read 2477440 spots for SRR26075348.sra Written 2477440 spots for SRR26075348.sra Read 2477440 spots for SRR26075348.sra Written 2477440 spots for SRR26075348.sra Read 2477440 spots for SRR26075348.sra Written 2477440 spots for SRR26075348.sra Read 2477440 spots for SRR26075348.sra Written 2477440 spots for SRR26075348.sra Read 2477440 spots for SRR26075348.sra Written 2477440 spots for SRR26075348.sra Read 2477440 spots for SRR26075348.sra Written 2477440 spots for SRR26075348.sra Read 2477440 spots for SRR26075348.sra Written 2477440 spots for SRR26075348.sra Read 2477440 spots for SRR26075348.sra Written 2477440 spots for SRR26075348.sra Read 2477440 spots for SRR26075348.sra Written 2477440 spots for SRR26075348.sra Read 2477440 spots for SRR26075348.sra Written 2477440 spots for SRR26075348.sra Read 2477440 spots for SRR26075348.sra Written 2477440 spots for SRR26075348.sra Read 2477440 spots for SRR26075348.sra Written 2477440 spots for SRR26075348.sra Read 2477440 spots for SRR26075348.sra Written 2477440 spots for SRR26075348.sra SRR ids: ['SRR26075348.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_brz9ep6g SRR26075348.sra spots: 49548807 blocks: [[1, 2477440], [2477441, 4954880], [4954881, 7432320], [7432321, 9909760], [9909761, 12387200], [12387201, 14864640], [14864641, 17342080], [17342081, 19819520], [19819521, 22296960], [22296961, 24774400], [24774401, 27251840], [27251841, 29729280], [29729281, 32206720], [32206721, 34684160], [34684161, 37161600], [37161601, 39639040], [39639041, 42116480], [42116481, 44593920], [44593921, 47071360], [47071361, 49548807]] SRR26075348 file size 18302152 SRR26075348 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o SRR26075348 SRR26075348_1.fastq SRR26075348_2.fastq Input file: SRR26075348_1.fastq Paired file: SRR26075348_2.fastq trimmed: SRR26075348-trimmed-pair1.fastq, SRR26075348-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Wed Feb 12 02:17:06 2025 >> started Wed Feb 12 02:18:10 2025 >> done (64.174s) 49548807 read pairs processed; of these: 164 ( 0.00%) short read pairs filtered out after trimming by size control 131388 ( 0.27%) empty read pairs filtered out after trimming by size control 49417255 (99.73%) read pairs available; of these: 7068180 (14.30%) trimmed read pairs available after processing 42349075 (85.70%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 18 28 0.00% 19 16 0.00% 20 13 0.00% 21 30 0.00% 22 34 0.00% 23 20 0.00% 24 43 0.00% 25 38 0.00% 26 44 0.00% 27 51 0.00% 28 71 0.00% 29 43 0.00% 30 61 0.00% 31 62 0.00% 32 81 0.00% 33 66 0.00% 34 76 0.00% 35 67 0.00% 36 71 0.00% 37 93 0.00% 38 91 0.00% 39 95 0.00% 40 96 0.00% 41 124 0.00% 42 124 0.00% 43 150 0.00% 44 115 0.00% 45 153 0.00% 46 152 0.00% 47 212 0.00% 48 186 0.00% 49 238 0.00% 50 300 0.00% 51 313 0.00% 52 367 0.00% 53 315 0.00% 54 412 0.00% 55 443 0.00% 56 537 0.00% 57 529 0.00% 58 590 0.00% 59 730 0.00% 60 821 0.00% 61 1010 0.00% 62 1051 0.00% 63 1209 0.00% 64 1311 0.00% 65 1535 0.00% 66 1592 0.00% 67 1734 0.00% 68 2126 0.00% 69 2508 0.01% 70 2767 0.01% 71 3371 0.01% 72 3761 0.01% 73 4149 0.01% 74 4757 0.01% 75 5407 0.01% 76 5908 0.01% 77 6554 0.01% 78 7253 0.01% 79 8102 0.02% 80 9405 0.02% 81 10410 0.02% 82 11812 0.02% 83 13205 0.03% 84 15227 0.03% 85 16602 0.03% 86 18153 0.04% 87 19228 0.04% 88 20338 0.04% 89 22631 0.05% 90 24555 0.05% 91 26923 0.05% 92 30029 0.06% 93 32401 0.07% 94 35341 0.07% 95 38019 0.08% 96 40330 0.08% 97 42619 0.09% 98 44620 0.09% 99 46458 0.09% 100 48813 0.10% 101 51820 0.10% 102 56176 0.11% 103 58810 0.12% 104 63552 0.13% 105 66418 0.13% 106 68500 0.14% 107 72058 0.15% 108 74472 0.15% 109 76157 0.15% 110 77463 0.16% 111 81832 0.17% 112 84879 0.17% 113 88991 0.18% 114 93258 0.19% 115 98494 0.20% 116 102052 0.21% 117 104783 0.21% 118 104985 0.21% 119 105625 0.21% 120 111034 0.22% 121 112135 0.23% 122 116513 0.24% 123 120984 0.24% 124 125063 0.25% 125 129647 0.26% 126 134533 0.27% 127 135767 0.27% 128 136296 0.28% 129 138812 0.28% 130 141675 0.29% 131 143373 0.29% 132 148296 0.30% 133 152117 0.31% 134 154751 0.31% 135 161794 0.33% 136 162819 0.33% 137 165136 0.33% 138 167703 0.34% 139 169580 0.34% 140 174272 0.35% 141 173857 0.35% 142 179082 0.36% 143 179601 0.36% 144 187250 0.38% 145 190728 0.39% 146 192208 0.39% 147 196812 0.40% 148 197623 0.40% 149 196133 0.40% 150 200936 0.41% 151 42349075 85.70% 49417255 reads passed initial QC criterion=sequence-density sequence-density=0.29 sequence-density-rank=1 fanout-score=4.40 fanout-score-rank=14 prefix-density=0.39 prefix-fanout=3.3 sequence=AGGAAACCTCCT criterion=fanout-score sequence-density=0.02 sequence-density-rank=37 fanout-score=31.08 fanout-score-rank=1 prefix-density=0.11 prefix-fanout=5.3 sequence=TTATTTATTTAAGCAAAGTACCCAGCAACGCCACACATATGAATAGCAAGTAAGAGACTGGCCATGACATGGTTGCTGCATTTTTCTTTTGCGCGAGTTGCATTATTTCACTAACAAGGCTTGGCGCAAAAACAGCGGCGGCGGAACCCTTTGCATTCACCACCGGGAAAACCTTCAGTCTTGCACACACTAGCACAGTTGTGGCCTCTTACACATGGTCCTTTAAAACTATGGCTCTGTGACAAACAAACCCTAGCCTCAGCAGGTACCATCATTTCCTGGGAAGCCAGGGCAATGAGCAGCAACAAGAAAAGACCATAGCATTTCTTCTCCATCTCTCTGTCTAACAAGAACCTGTCTAACACTAGCTCTCTGTCTGCAACTACTACTAGT criterion=sequence-density sequence-density=0.41 sequence-density-rank=1 fanout-score=5.13 fanout-score-rank=9 prefix-density=0.54 prefix-fanout=3.9 sequence=AGAAAACAATGG criterion=fanout-score sequence-density=0.12 sequence-density-rank=25 fanout-score=19.62 fanout-score-rank=1 prefix-density=0.37 prefix-fanout=6.1 sequence=TGAAGATGTCTCATCCAGAAATCACAAACAGATGGTCTCTCCAGGGAACCACTGCTCTTGTCACTGGTGGAACCAAAGGGATCGGATATGCTGTTGTGGAAGAATTGGCAGCCCTAGGAGCATGTGTTCATACATGTGCGAGGACCCAAGGCCAGATTGATGCATGCTTACGTCAATGGAAGGAGAGGGGTCTTAAAGTTAGCGGATCAGTCTGTGATGTATCCTCTCAAGCTGACCGAGAGAAGCTGATAAAGGAGGTTTCC SRR26075348 testing PE reads STAR mapping to Ensembl genome Started job on | Feb 12 02:19:00 Started mapping on | Feb 12 02:19:01 Finished on | Feb 12 02:29:07 Mapping speed, Million of reads per hour | 293.57 Number of input reads | 49417255 Average input read length | 295 UNIQUE READS: Uniquely mapped reads number | 43326381 Uniquely mapped reads % | 87.67% Average mapped length | 293.43 Number of splices: Total | 41250485 Number of splices: Annotated (sjdb) | 40316757 Number of splices: GT/AG | 40521669 Number of splices: GC/AG | 563713 Number of splices: AT/AC | 34699 Number of splices: Non-canonical | 130404 Mismatch rate per base, % | 0.39% Deletion rate per base | 0.03% Deletion average length | 3.13 Insertion rate per base | 0.02% Insertion average length | 2.64 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 1495586 % of reads mapped to multiple loci | 3.03% Number of reads mapped to too many loci | 137814 % of reads mapped to too many loci | 0.28% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 8.70% % of reads unmapped: other | 0.32% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 4595288 4595288 4595288 N_multimapping 1495586 1495586 1495586 N_noFeature 1098888 42830447 1375610 N_ambiguous 458514 2224 237768 UnstrandedReadsAssigned:41768979 PositiveStrandReadsAssigned:493710 NegativeStrandReadsAssigned:41713003 Dataset is classified negative stranded MeadianReadLen=151 20thPercentileLength=151 echo kmer=147 SRR26075348 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,400 [index] number of k-mers: 62,057,036 [index] number of equivalence classes: 130,681 [quant] running in paired-end mode [quant] will process pair 1: SRR26075348-trimmed-pair1.fastq SRR26075348-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 49,417,255 reads, 42,350,980 reads pseudoaligned [quant] estimated average fragment length: 216.909 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,200 rounds 52401 SRR26075348.ke.tsv 34699 SRR26075348.se.tsv 87100 total ==> SRR26075348.ke.tsv <== target_id length eff_length est_counts tpm Potri.005G200100.1.v4.1 2018 1802.09 4564 57.0887 Potri.005G024800.1.v4.1 1035 819.091 2038 56.0859 Potri.004G059700.1.v4.1 961 745.102 6 0.181517 Potri.007G009000.2.v4.1 1416 1200.09 0 0 Potri.003G141000.2.v4.1 2943 2727.09 1977.41 16.3447 Potri.016G087400.1.v4.1 270 88.3224 3324 848.343 Potri.015G069301.1.v4.1 564 350.454 0 0 Potri.010G195200.1.v4.1 1773 1557.09 618 8.94655 Potri.012G127500.1.v4.1 977 761.091 13821 409.34 ==> SRR26075348.se.tsv <== Potri.001G166300.v4.1 0 Potri.001G448400.v4.1 771 Potri.001G233950.v4.1 0 Potri.001G122700.v4.1 595 Potri.001G212900.v4.1 0 Potri.001G182400.v4.1 0 Potri.001G256600.v4.1 0 Potri.001G040500.v4.1 0 Potri.001G416900.v4.1 0 Potri.001G452600.v4.1 1563 SRR26075348 completed mapping pipeline successfully