Starting /dee2/code/volunteer_pipeline.sh SRR26075349 current disk space = 3049123733504 free memory = 1578902976 SRR26075349 SRAfilesize 042d706481381f3cbd19c2a500402631 SRR26075349.sra SRR26075349.sra file validated SRR26075349 is paired end SRR26075349 is conventional basespace SRR26075349 read1 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR26075349_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.5785 37.0 37.0 37.0 37.0 37.0 2 36.65 37.0 37.0 37.0 37.0 37.0 3 36.662 37.0 37.0 37.0 37.0 37.0 4 36.613 37.0 37.0 37.0 37.0 37.0 5 36.7155 37.0 37.0 37.0 37.0 37.0 6 36.687 37.0 37.0 37.0 37.0 37.0 7 36.627 37.0 37.0 37.0 37.0 37.0 8 36.623 37.0 37.0 37.0 37.0 37.0 9 36.684 37.0 37.0 37.0 37.0 37.0 10-14 36.6488 37.0 37.0 37.0 37.0 37.0 15-19 36.6632 37.0 37.0 37.0 37.0 37.0 20-24 36.537699999999994 37.0 37.0 37.0 37.0 37.0 25-29 36.4836 37.0 37.0 37.0 37.0 37.0 30-34 36.4462 37.0 37.0 37.0 37.0 37.0 35-39 36.3797 37.0 37.0 37.0 37.0 37.0 40-44 36.341499999999996 37.0 37.0 37.0 37.0 37.0 45-49 36.1849 37.0 37.0 37.0 37.0 37.0 50-54 36.151399999999995 37.0 37.0 37.0 37.0 37.0 55-59 36.036199999999994 37.0 37.0 37.0 37.0 37.0 60-64 36.0538 37.0 37.0 37.0 37.0 37.0 65-69 35.966899999999995 37.0 37.0 37.0 37.0 37.0 70-74 36.0544 37.0 37.0 37.0 37.0 37.0 75-79 36.0522 37.0 37.0 37.0 37.0 37.0 80-84 35.9953 37.0 37.0 37.0 37.0 37.0 85-89 35.943599999999996 37.0 37.0 37.0 37.0 37.0 90-94 35.8569 37.0 37.0 37.0 37.0 37.0 95-99 35.812799999999996 37.0 37.0 37.0 37.0 37.0 100-104 35.7953 37.0 37.0 37.0 37.0 37.0 105-109 35.6737 37.0 37.0 37.0 37.0 37.0 110-114 35.61729999999999 37.0 37.0 37.0 37.0 37.0 115-119 35.4654 37.0 37.0 37.0 34.6 37.0 120-124 35.4576 37.0 37.0 37.0 37.0 37.0 125-129 35.3655 37.0 37.0 37.0 34.6 37.0 130-134 35.2308 37.0 37.0 37.0 29.8 37.0 135-139 35.0143 37.0 37.0 37.0 29.8 37.0 140-144 34.763799999999996 37.0 37.0 37.0 25.0 37.0 145-149 34.7336 37.0 37.0 37.0 25.0 37.0 150-151 34.632999999999996 37.0 37.0 37.0 25.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.0 1101 2 0.0 1101 3 0.0 1101 4 0.0 1101 5 0.0 1101 6 0.0 1101 7 0.0 1101 8 0.0 1101 9 0.0 1101 10-14 0.0 1101 15-19 0.0 1101 20-24 0.0 1101 25-29 0.0 1101 30-34 0.0 1101 35-39 0.0 1101 40-44 0.0 1101 45-49 0.0 1101 50-54 0.0 1101 55-59 0.0 1101 60-64 0.0 1101 65-69 0.0 1101 70-74 0.0 1101 75-79 0.0 1101 80-84 0.0 1101 85-89 0.0 1101 90-94 0.0 1101 95-99 0.0 1101 100-104 0.0 1101 105-109 0.0 1101 110-114 0.0 1101 115-119 0.0 1101 120-124 0.0 1101 125-129 0.0 1101 130-134 0.0 1101 135-139 0.0 1101 140-144 0.0 1101 145-149 0.0 1101 150-151 0.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 19 1.0 20 0.0 21 0.0 22 9.0 23 2.0 24 8.0 25 3.0 26 7.0 27 18.0 28 19.0 29 28.0 30 27.0 31 45.0 32 77.0 33 121.0 34 179.0 35 515.0 36 2769.0 37 172.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.817269076305216 14.206827309236946 7.254016064257028 36.721887550200805 2 19.225 13.55 35.025 32.2 3 15.6 16.6 28.9 38.9 4 19.950000000000003 24.075 24.9 31.075000000000003 5 22.425 27.925 26.35 23.3 6 21.9 32.7 24.7 20.7 7 16.55 28.95 37.175000000000004 17.325 8 15.275 28.849999999999998 31.5 24.375 9 19.575 23.75 33.525 23.150000000000002 10-14 19.656965696569657 28.85788578857886 27.892789278927893 23.592359235923592 15-19 18.975 27.694999999999997 28.175 25.155 20-24 19.91 27.055 29.38 23.655 25-29 20.28 26.400000000000002 28.444999999999997 24.875 30-34 19.74 26.91 28.444999999999997 24.905 35-39 19.235 26.86 28.955 24.95 40-44 19.03 27.894999999999996 27.994999999999997 25.080000000000002 45-49 20.45 26.669999999999998 28.23 24.65 50-54 19.915 26.834999999999997 28.505000000000003 24.745 55-59 20.48 25.995 28.754999999999995 24.77 60-64 20.305 26.584999999999997 28.645 24.465 65-69 20.485 27.35 28.185 23.98 70-74 20.82 27.500000000000004 27.405 24.275 75-79 21.02 27.05 27.589999999999996 24.34 80-84 20.14 27.145000000000003 28.475 24.240000000000002 85-89 20.935000000000002 25.814999999999998 28.199999999999996 25.05 90-94 20.745 27.665 27.275 24.315 95-99 20.75 27.215 27.439999999999998 24.595 100-104 21.625 25.929999999999996 28.000000000000004 24.445 105-109 20.080000000000002 27.38 27.54 25.0 110-114 21.07 26.43 27.375 25.124999999999996 115-119 20.905 26.665 27.42 25.009999999999998 120-124 21.725 26.229999999999997 27.445000000000004 24.6 125-129 21.215 26.495 27.46 24.83 130-134 20.945 26.76 27.295 25.0 135-139 21.01 27.775 27.08 24.135 140-144 21.685 26.729999999999997 26.784999999999997 24.8 145-149 23.035 26.44 26.19 24.335 150-151 21.0375 28.1875 25.912499999999998 24.8625 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.5 4 1.5 5 1.5 6 0.5 7 0.0 8 0.5 9 0.5 10 0.5 11 1.0 12 0.5 13 0.0 14 0.0 15 0.0 16 0.5 17 1.0 18 1.0 19 0.5 20 0.0 21 2.0 22 4.5 23 3.0 24 1.0 25 2.5 26 4.5 27 5.0 28 7.5 29 13.5 30 15.5 31 21.0 32 21.5 33 31.0 34 40.0 35 44.0 36 68.5 37 81.5 38 87.5 39 105.0 40 165.0 41 188.5 42 199.5 43 260.0 44 277.5 45 290.0 46 301.0 47 283.5 48 272.5 49 246.0 50 190.5 51 161.0 52 132.5 53 105.5 54 84.0 55 51.5 56 47.5 57 36.0 58 21.0 59 16.0 60 18.0 61 17.0 62 12.5 63 9.0 64 4.0 65 5.0 66 3.5 67 4.0 68 7.0 69 8.5 70 4.0 71 2.5 72 3.5 73 1.5 74 1.0 75 1.0 76 0.0 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.4 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.01 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150-151 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 61.45 #Duplication Level Percentage of deduplicated Percentage of total 1 64.27990235964198 39.5 2 21.358828315703825 26.25 3 7.689178193653377 14.174999999999999 4 3.7428803905614325 9.2 5 1.4646053702196908 4.5 6 0.5695687550854354 2.1 7 0.40683482506102525 1.7500000000000002 8 0.2847843775427177 1.4000000000000001 9 0.20341741253051263 1.125 >10 0.0 0.0 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source TGTAGTTCGATGGTCTGGACGAACTTGCGGGGCTTCTCATTCACACCATT 9 0.22499999999999998 No Hit ATTACATTAGTGCATCAAGTTGGAACATACAAAACAAAATCACACGATCG 9 0.22499999999999998 No Hit CTTGTCTTCCTTCTCCACGTTCCTCTGTCCGCTAATCTGGAGCACTCTGT 9 0.22499999999999998 No Hit CACAGAGTCACAGACAGGCACCCAATTCTCACAATGGATGTATAATCTCA 9 0.22499999999999998 No Hit CTTCTTCTTCTTCAACAAACCCAACTGGCACCGTTGATCCCTCTTCTTCC 9 0.22499999999999998 No Hit GCTACAAGCGAGAGAGATATGAGTTCATGGGGAAGTAAGAAACTACGATT 8 0.2 No Hit GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGTACCGTATCTCGTAT 8 0.2 TruSeq Adapter, Index 22 (97% over 38bp) CTAGGAGACCATTTAGAGGCTTCATTTCAACTTTGGGATCGGTAGCACTG 8 0.2 No Hit CCTGGATTTAGTCTTCACAGGAATAGGATTTTGCTGGTCGGATTGACCAT 8 0.2 No Hit GGCCAGTCCAGTCCCTCATAGAGACCTTCACCAGAAGTGGCACAGGTGCT 8 0.2 No Hit GCCACAGCCGTCGGCTCAGGCATCAACCGGAGGATGTGGAGGCCAGCCAT 8 0.2 No Hit GTCTTTGTATCTTATCAGCAGGTTTGTGCGCTTCTTGATCACTTCTGGAT 8 0.2 No Hit GACGTTATAAAGAGATAGAAACCAATCATTATAAGCTGGTTGGCCTGAGA 7 0.17500000000000002 No Hit CCCATCATTAATGAACAGCGAGCTTGCGCCTATAAATTTATAAATTATAC 7 0.17500000000000002 No Hit GGTAGAGTGACTGCACAGACAAGCTCATATTGCGCTCCAAATTTGAGTGC 7 0.17500000000000002 No Hit GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGTACCGTATCTCGGTT 7 0.17500000000000002 TruSeq Adapter, Index 22 (97% over 38bp) GCTTGTCATTTCATCGCCACTCTTAGTTGAATGGTACCGGAGCAAATCAG 7 0.17500000000000002 No Hit GAAAAATTCAGGACACCATTAGGCCTCCCATCGCTATTTCGGACCTGATA 7 0.17500000000000002 No Hit CCTGCACCGCTGCCACCAGATGGAGGAGCATCATCATCCATGCCACCACC 7 0.17500000000000002 No Hit ACAGAGTCACAGACAGGCACCCAATTCTCACAATGGATGTATAATCTCAC 7 0.17500000000000002 No Hit GTCAAGTTTCAACCCTTCCTTTGTAGCAGAAACCAGCTTCTTGCCGTCAT 7 0.17500000000000002 No Hit CTTCAACTTGTCCTCAAATTCATCTACTTCTGCCAATTGGTTCCTGTCTA 7 0.17500000000000002 No Hit ATCTTGAAGAGTTTACCTTCATACTATCTGAATCTTTTATTCCCCTCTTC 6 0.15 No Hit GGTCTTTCAGGATTGGTCGTTGTGTGTCACCACCTCCCACCTATCCTACA 6 0.15 No Hit ACAAGCCATACACGAGCTCCTTTTTTGTTAGGGTCACGGATCTCAGCTGC 6 0.15 No Hit ATCCGACAAAAGTAGTTATAAATAGCCTGTGCATCACCCCTTTTAAGGTT 6 0.15 No Hit GCCTCAGCTTATCAACATCCATTACATTCCGTCCATCAAAAACAAAAGCA 6 0.15 No Hit GTACAGAGAGTATCCACTTGCAGCTTGAAGGCATTGCCTCCAGCGGTAAT 6 0.15 No Hit ATCGGCGTAGTGGTGGAGAGACGCGTGAGCCGCCGACCGGTTAGAGTAGA 6 0.15 No Hit GGCTCTTAAGACGGTATCCAATTCTGAGAGGGGCTGCACAAAAGCATGGC 6 0.15 No Hit GGAAAGAGAGAGACGTGCTCTGGCTCTGGCTCTGGCTCTGGCTCTGGCTC 6 0.15 No Hit CCCCTCAGGATCATACATTTCCCAGAATCCTTTGATAGCAACCTCACGGA 6 0.15 No Hit AGCGTCGCAGTGTCATCCATCGTCTCAGCCACACGTATCTCCTCGGCATG 6 0.15 No Hit GAGGGATTGGCGTGAGCATTTCTAGCAGGTTTCTTTGCCTTTTGGCAGAA 6 0.15 No Hit GCAACTCCTATCCGCATAGGCCTACTTGAGCAAAAAGCACCATTCATCTC 6 0.15 No Hit GTCCATGAGAAGACATATCCGTCCTGCCTCTATTAATACTGGACCTTGCG 6 0.15 No Hit GCATGGTTTGCAGCTCACGGTTCTTATACCTCTTATCTTGAAGAACTTTC 5 0.125 No Hit GCATGTCATATTTCGAGATTGTAATATATATATATAATTTCAGCTATTAC 5 0.125 No Hit CCCAGTTCTGCGTCAAGAACCCATACCGACTCCCGATAATCTCGGCGTCA 5 0.125 No Hit CTTCGAATCTTTGTCCTTGCCTTTCTTCTTCTCTTTTTTATCTTTCTTTT 5 0.125 No Hit CTGGAATTGAGGTGGAAGACATCTCATTGCGGTATGCACTTTCTGTGTAG 5 0.125 No Hit GCCGGAGTTTGACCGGAGACCGTAGCTCGGTTTCTTCCGTAGCTTCCCGA 5 0.125 No Hit CTCAATCTTCAAAATCTCCAGCATTTTCCAATAGATAGTTGGCTGCCAGT 5 0.125 No Hit GCATCATGTCTTTCCCATTCTTTCTAATTGCGACTCATCATCCTTCGATT 5 0.125 No Hit GGCCTTCCTATTTGGAAGCAGGGATTCATAATTGTTCATCACTCCCAAGC 5 0.125 No Hit CCCAAACAGACCCTTTACCTGATCGACAATATCGGGAACTAACGGAGCCA 5 0.125 No Hit CTCTTGGCGGCTTGCCTGACAATGTAAGCTCCACTCCTATCCACCTTGGT 5 0.125 No Hit GGATGTGCCAGGGGGGAAAAAGAGCCTTACATGCAAGTTTTCCCAAACAA 5 0.125 No Hit CCCCACTCGATTTCACCAAACCAGAAGAAGATAAAATCACACCAGAAGTA 5 0.125 No Hit CCCACATTCTTTAACGCTTGGTTTCAATGATTTACCCTTGCGCAGTGCAT 5 0.125 No Hit ACCACATCCAACAGTCTCAGATTTGGCTAAAACCATAATATCTGCTATCG 5 0.125 No Hit GCGCAGAAGGTATCTAAAGATATACATCGCCACCGCCGATCAAGAACCCA 5 0.125 No Hit CCTTCATCTTGTCCTCAAACTCATCTGCTTCTGCAAGCTGGTTGGTGTCT 5 0.125 No Hit GCACAGCATATTTACATTCATATTCCCAATCACACGACAGCACAAAATCT 5 0.125 No Hit GGGAGAATGAGGAGAAGTAGTGCTTGATGCAGTAACCTTGGCACTGAATT 5 0.125 No Hit CTCAAAACAAACAGGGCAATTATGGTGCATAGCCCTTTCAACACAACGGT 5 0.125 No Hit GTTCTGTTGCCTTGATCGTTAGCTATGATTTCCACTCTGTCGTGTTGCCA 5 0.125 No Hit TCTCTCTTGAGGTACTCACTAAAGTGAGACTGGTATTTCTCAGGTTCATC 5 0.125 No Hit GCTATATGAAAATTACTTGAGAACAACCAGCAAGCAGGAAGAAGAGCCCT 5 0.125 No Hit ACAGAGAGAAAGAAACAGCTTTTGGGGGTGCGGTGCAGGTGCTCGCAACA 5 0.125 No Hit GCCTCAGACTCAGCAGCACCATTCTGCAATTCATCAGCAGCATTAGCAAC 5 0.125 No Hit GAGCTGGAAGAGAAACTGGGTGGTTATTGTCGGGTTTCCGGGGAGATAGT 5 0.125 No Hit GTTAAAATCAGGAACCTCTGTGATGTCCACCAGGTATATCACAGCAAAAT 5 0.125 No Hit CAGCTGGTGTTGATGCCATTGCCTTGGCATCACGTACGTAAGTGCGTGTT 5 0.125 No Hit CTGGCAAGAAAAGCATAAGAGAGGAATCATAGAATAGAGCAAGAGGAAGA 5 0.125 No Hit CACACTAAATACCATGAATTTCATATCATATCCTCCAAATACAGTTTCGA 5 0.125 No Hit GCCGGATCTAAGAATTTAATAGTTTCTTGATTCCTGACTTCTGCTCAGCA 5 0.125 No Hit CTCCTTTCCAAGTCAATGAAGCTATTTACCATCACCCCCTCAGCCAATCT 5 0.125 No Hit CCTAGTAGAGTATTGTGTTTTCAACCCATGGAAAAGCCGTTCACTGACAA 5 0.125 No Hit CTCCATTCTTTGAAATACTGTGACGCTGATTTCTCTTCCATGCCTTTTGC 5 0.125 No Hit CAGGTTTTTTGACTTCCTCTTTAGGCACAGTCACAGTCAAGACCCCGTTT 5 0.125 No Hit AGGGTACATCTTGCATCCTCCACAGCCGCTACCGCACTTGCAGCCAGAGC 5 0.125 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0 0.0 0.0 0.0 0.0 64-65 0.0 0.0 0.0 0.0 0.0 66-67 0.0375 0.0 0.0 0.0 0.0 68-69 0.0875 0.0 0.0 0.0 0.0 70-71 0.1125 0.0 0.0 0.0 0.0 72-73 0.1875 0.0 0.0 0.0 0.0 74-75 0.25 0.0 0.0 0.0 0.0 76-77 0.275 0.0 0.0 0.0 0.0 78-79 0.3875 0.0 0.0 0.0 0.0 80-81 0.425 0.0 0.0 0.0 0.0 82-83 0.5125 0.0 0.0 0.0 0.0 84-85 0.6499999999999999 0.0 0.0 0.0 0.0 86-87 0.8 0.0 0.0 0.0 0.0 88-89 0.9624999999999999 0.0 0.0 0.0 0.0 90-91 1.1749999999999998 0.0 0.0 0.0 0.0 92-93 1.3250000000000002 0.0 0.0 0.0 0.0 94-95 1.6124999999999998 0.0 0.0 0.0 0.0 96-97 1.7625 0.0 0.0 0.0 0.0 98-99 1.9625 0.0 0.0 0.0 0.0 100-101 2.2 0.0 0.0 0.0 0.0 102-103 2.3625 0.0 0.0 0.0 0.0 104-105 2.6375 0.0 0.0 0.0 0.0 106-107 3.1375 0.0 0.0 0.0 0.0 108-109 3.4625 0.0 0.0 0.0 0.0 110-111 3.825 0.0 0.0 0.0 0.0 112-113 4.2875 0.0 0.0 0.0 0.0 114-115 4.737500000000001 0.0 0.0 0.0 0.0 116-117 5.0875 0.0 0.0 0.0 0.0 118-119 5.4375 0.0 0.0 0.0 0.0 120-121 5.7875 0.0 0.0 0.0 0.0 122-123 6.35 0.0 0.0 0.0 0.0 124-125 6.8375 0.0 0.0 0.0 0.0 126-127 7.45 0.0 0.0 0.0 0.0 128-129 8.625 0.0 0.0 0.0 0.0 130-131 9.600000000000001 0.0 0.0 0.0 0.0 132-133 10.3125 0.0 0.0 0.0 0.0 134-135 10.9625 0.0 0.0 0.0 0.0 136-137 11.575 0.0 0.0 0.0 0.0 138-139 12.1875 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content pass >>END_MODULE SRR26075349 read2 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR26075349_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.3625 37.0 37.0 37.0 37.0 37.0 2 36.173 37.0 37.0 37.0 37.0 37.0 3 36.272 37.0 37.0 37.0 37.0 37.0 4 36.164 37.0 37.0 37.0 37.0 37.0 5 36.3145 37.0 37.0 37.0 37.0 37.0 6 36.1795 37.0 37.0 37.0 37.0 37.0 7 36.2285 37.0 37.0 37.0 37.0 37.0 8 36.2375 37.0 37.0 37.0 37.0 37.0 9 36.3475 37.0 37.0 37.0 37.0 37.0 10-14 36.23610000000001 37.0 37.0 37.0 37.0 37.0 15-19 36.1537 37.0 37.0 37.0 37.0 37.0 20-24 36.19760000000001 37.0 37.0 37.0 37.0 37.0 25-29 36.0138 37.0 37.0 37.0 37.0 37.0 30-34 35.844100000000005 37.0 37.0 37.0 37.0 37.0 35-39 35.7825 37.0 37.0 37.0 37.0 37.0 40-44 35.7622 37.0 37.0 37.0 37.0 37.0 45-49 35.6288 37.0 37.0 37.0 37.0 37.0 50-54 35.4821 37.0 37.0 37.0 37.0 37.0 55-59 35.5023 37.0 37.0 37.0 37.0 37.0 60-64 35.5321 37.0 37.0 37.0 37.0 37.0 65-69 35.535 37.0 37.0 37.0 37.0 37.0 70-74 35.4685 37.0 37.0 37.0 37.0 37.0 75-79 35.376999999999995 37.0 37.0 37.0 37.0 37.0 80-84 35.4441 37.0 37.0 37.0 37.0 37.0 85-89 35.4145 37.0 37.0 37.0 37.0 37.0 90-94 35.30309999999999 37.0 37.0 37.0 37.0 37.0 95-99 35.356399999999994 37.0 37.0 37.0 37.0 37.0 100-104 35.2877 37.0 37.0 37.0 37.0 37.0 105-109 35.2023 37.0 37.0 37.0 32.2 37.0 110-114 35.1596 37.0 37.0 37.0 29.8 37.0 115-119 35.1825 37.0 37.0 37.0 29.8 37.0 120-124 35.1034 37.0 37.0 37.0 29.8 37.0 125-129 35.0478 37.0 37.0 37.0 27.4 37.0 130-134 35.018899999999995 37.0 37.0 37.0 29.8 37.0 135-139 34.851800000000004 37.0 37.0 37.0 25.0 37.0 140-144 34.835499999999996 37.0 37.0 37.0 25.0 37.0 145-149 34.7097 37.0 37.0 37.0 25.0 37.0 150-151 34.47275 37.0 37.0 37.0 25.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.0 1101 2 0.0 1101 3 0.0 1101 4 0.0 1101 5 0.0 1101 6 0.0 1101 7 0.0 1101 8 0.0 1101 9 0.0 1101 10-14 0.0 1101 15-19 0.0 1101 20-24 0.0 1101 25-29 0.0 1101 30-34 0.0 1101 35-39 0.0 1101 40-44 0.0 1101 45-49 0.0 1101 50-54 0.0 1101 55-59 0.0 1101 60-64 0.0 1101 65-69 0.0 1101 70-74 0.0 1101 75-79 0.0 1101 80-84 0.0 1101 85-89 0.0 1101 90-94 0.0 1101 95-99 0.0 1101 100-104 0.0 1101 105-109 0.0 1101 110-114 0.0 1101 115-119 0.0 1101 120-124 0.0 1101 125-129 0.0 1101 130-134 0.0 1101 135-139 0.0 1101 140-144 0.0 1101 145-149 0.0 1101 150-151 0.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 13 4.0 14 6.0 15 9.0 16 11.0 17 6.0 18 4.0 19 3.0 20 4.0 21 10.0 22 10.0 23 18.0 24 17.0 25 13.0 26 17.0 27 15.0 28 23.0 29 22.0 30 22.0 31 38.0 32 62.0 33 88.0 34 197.0 35 698.0 36 2507.0 37 196.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 46.6 20.724999999999998 10.7 21.975 2 28.599999999999998 27.800000000000004 24.825 18.775 3 23.974999999999998 28.675 28.575 18.775 4 25.5 33.75 21.625 19.125 5 26.150000000000002 38.275 19.925 15.65 6 20.95 40.225 21.825 17.0 7 25.174999999999997 21.275 35.199999999999996 18.35 8 23.549999999999997 26.950000000000003 25.15 24.349999999999998 9 23.5 26.875 26.950000000000003 22.675 10-14 25.465 30.395 24.165 19.975 15-19 25.445 29.62 25.569999999999997 19.365 20-24 25.715 28.970000000000002 25.840000000000003 19.475 25-29 24.935 28.95 25.374999999999996 20.74 30-34 25.795 29.099999999999998 25.580000000000002 19.525000000000002 35-39 26.565 28.720000000000002 24.425 20.29 40-44 25.635 28.51 25.240000000000002 20.615 45-49 25.014999999999997 29.720000000000002 25.259999999999998 20.005 50-54 25.174999999999997 28.04 26.82 19.965 55-59 26.035000000000004 27.01 26.51 20.445 60-64 25.86 29.060000000000002 25.905 19.175 65-69 25.729999999999997 28.199999999999996 26.115 19.955000000000002 70-74 26.8 27.49 26.165 19.545 75-79 24.92 29.385 25.585 20.11 80-84 25.740000000000002 28.935 25.81 19.515 85-89 25.365 29.48 25.005 20.150000000000002 90-94 25.580000000000002 28.665000000000003 25.66 20.095 95-99 25.285000000000004 28.999999999999996 25.44 20.275000000000002 100-104 25.235000000000003 28.860000000000003 25.935000000000002 19.97 105-109 26.284999999999997 28.765 25.645 19.305 110-114 26.055 28.125 26.165 19.655 115-119 24.965 29.12 25.569999999999997 20.345 120-124 26.58 28.15 26.46 18.81 125-129 26.534999999999997 28.345 25.130000000000003 19.99 130-134 26.784999999999997 28.64 25.619999999999997 18.955 135-139 26.77 28.185 26.38 18.665000000000003 140-144 27.26 28.22 25.795 18.725 145-149 27.445000000000004 27.534999999999997 26.38 18.64 150-151 28.050000000000004 27.737499999999997 24.5125 19.7 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.5 8 0.5 9 1.0 10 1.0 11 0.0 12 1.5 13 1.5 14 0.5 15 1.0 16 0.5 17 0.0 18 0.0 19 0.5 20 1.0 21 1.5 22 1.5 23 0.5 24 0.5 25 2.5 26 3.5 27 5.0 28 6.5 29 7.5 30 7.5 31 9.0 32 11.5 33 19.5 34 32.0 35 45.0 36 56.5 37 74.5 38 108.5 39 148.0 40 177.5 41 215.0 42 258.5 43 271.0 44 276.5 45 286.5 46 277.0 47 269.0 48 246.0 49 210.5 50 196.0 51 162.5 52 120.5 53 109.0 54 83.0 55 50.0 56 40.5 57 32.5 58 26.0 59 16.5 60 10.0 61 6.5 62 3.0 63 2.5 64 7.0 65 6.0 66 6.0 67 6.0 68 1.5 69 1.0 70 0.5 71 1.0 72 0.5 73 0.5 74 3.0 75 3.0 76 0.5 77 2.0 78 3.5 79 1.5 80 0.5 81 2.0 82 2.0 83 1.5 84 1.5 85 2.5 86 3.5 87 2.5 88 2.5 89 1.5 90 2.0 91 3.5 92 3.5 93 2.5 94 1.0 95 1.0 96 1.0 97 1.5 98 1.0 99 1.5 100 11.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150-151 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 61.95 #Duplication Level Percentage of deduplicated Percentage of total 1 65.57707828894269 40.625 2 20.298627925746572 25.15 3 8.192090395480225 15.225 4 3.188054882970137 7.9 5 1.331719128329298 4.125 6 0.5649717514124294 2.1 7 0.20177562550443903 0.8750000000000001 8 0.36319612590799033 1.7999999999999998 9 0.20177562550443903 1.125 >10 0.08071025020177562 1.075 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG 31 0.775 No Hit GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGT 12 0.3 No Hit CCAAAAGATTGTATCCGACAAAGAAAGAAGAGAGAGATGGCGTTGATTCC 9 0.22499999999999998 No Hit CTTCTCTTCTCCAAGTTCCTCCATTTTTGTTCTTTGGACAAAACTCTGAA 9 0.22499999999999998 No Hit GCCTGAGAATGCTAAGGTGGATCAGGTCAAGGCTTCTATGGAGAATGGGG 9 0.22499999999999998 No Hit CCGGATGTTGAATGAGGATGAATTGAGGGATGCTGTGCTACTTGTGTTTG 9 0.22499999999999998 No Hit AAAACATCAAACAGTTGGGACAGTTCAATGAAGATGTTGAAGAGAAAAGA 9 0.22499999999999998 No Hit AAGCTCAAGAGTGATGAAGCAAAGCAAGAGCTCTAGATGTTTTCATGTAT 8 0.2 No Hit AGTGGACAGAGAAGGAGGTAGCAACCCTAAATGGAACCATATGATGCAGT 8 0.2 No Hit CCGGAGGATTTGGATGTTGGTGTTGAGGAGTTTTCTAACTTGGAAGCTCA 8 0.2 No Hit TCTACATCAACTCAAATTGCTGCAATGAGAGGAATGGGTTTTTATGAGCC 8 0.2 No Hit TCAGACTGCCTGAGAATGCTAAGGTGGATCAGGTCAAGGCTTCTATGGAG 8 0.2 No Hit TGATGTGCCGACATCGATGAGTGATTATTGTGATTCGGTGCGAGGAGTGG 8 0.2 No Hit GCAAAAGAAAGTTAACTAGTTGGCCTACAGTGTCTTCACCAATACTACTT 8 0.2 No Hit CCACAACCGAACTTCTCTCGTCCTTATCTCCATTCTTCCATAGCTCTCAT 8 0.2 No Hit AGTCCTTGCAATATTTCTGGTTGAACTTCGAGCCATGGCTGAACTTCAGT 8 0.2 No Hit GTCATTTTTGACATTTCTGTGTTGATATATTATTTTCTTCATCCATGGTG 7 0.17500000000000002 No Hit CAGTGAGAGATGACAAGGTTGGTGGTAAGTTGGACCCGGCCGACAAGCAG 7 0.17500000000000002 No Hit CAGTGGGGTTGAAGGAATGCAGGCAGTCATGTCAAGCGATATTGCAATTG 7 0.17500000000000002 No Hit CTCGTGAGATGCTTCAACAGAATAAAATCCTCAAGGTTATTAGGAAGAAC 7 0.17500000000000002 No Hit GACATTTACTATATTACTGGTGAAAGCAAGAAGGCAGTGGAGAACTCTCC 7 0.17500000000000002 No Hit GGAGGAACCCAAAAAAGAAGCCAAGGAAAAAGAGCCGGCAAAGCCCAAAG 6 0.15 No Hit GTTTAATTATGCCTGCTGCTGGTGTTACCTATATCCAGACACAGGAAAGA 6 0.15 No Hit GTTACTGGTCCGACGACTATGGTCCCCTACGCCATGTGCTTCATTCTCAC 6 0.15 No Hit CCCCAATCCTCACCAAAGTCTCCAAGCACTCTAAGCCAGAGAAAACCATC 6 0.15 No Hit GAAATTCCTTGTCGCATAAGTAGCGACCTGCACGAATGGTGTAACGACTG 6 0.15 No Hit CAGGGAATTCCGATGCAGAAAACCTATGGTCCTACCACTAAAACCCAGTC 6 0.15 No Hit GTTTGCATGGGGAAATACTACTGTTCTAAGTGCAAGTTCTTCGATGATGA 6 0.15 No Hit CTTCGATTTGCTCTCGTCTGTTATACCGCTCAGTCCGACAAAATAAATTT 6 0.15 No Hit GCATGGCCGAAGAAGCCAAATCCCGAGGCAACGCGGCCTTCTCAGCCCGC 6 0.15 No Hit GGATGCTTATGAGGCGACAAAGGGTGCTCACGGGATCTGCGTTCTAACTG 6 0.15 No Hit GCCCAAGAAGAGGGTGAAGCTTGGTAATGAGTCCGATGCACGCCCTTCGA 6 0.15 No Hit CTTGAACACAACCACTCGTTAGGGGCTAAGATATACAGACCTGTTAAGAA 6 0.15 No Hit GTTGCAGGAAACCTTTGCTGGAAAATATCCATCCGTCAAGGGAGCAAAGG 6 0.15 No Hit CTTACACTTGATCTCAGGAACAATTCGCTTTCAAGCATTTTAGGAGAAGT 6 0.15 No Hit GTTTGCTTGGTCGTGAAGAGCTCGACATGAGAGAGCCATTGCTTCCAGCT 5 0.125 No Hit GCATGGATTACTGAGGTTGATTTTTAGAGACTGATCCAACCTGCCTGACA 5 0.125 No Hit CATTAAAGAGGCGAAGAGAGAAGAAAACATAAAAAGAAACAATCAACAGT 5 0.125 No Hit CAGCACAATGCAATCTTTACTGATGCAAATGAGACTGTTTGGAGATGAAT 5 0.125 No Hit AGCTTTTATATCCCCTATTCTCTTCGTGAAGATGTCTTGCTGTGGAGGAA 5 0.125 No Hit GAGATGACAAAGAAATGGAGGCAACAGTGGTGGATGGTAATGGTACAGAG 5 0.125 No Hit CAGGAGGATAAGAAAGAGGGAAAAAAGAAAGATAAAAAAGAGAAGAAGAA 5 0.125 No Hit GGCGAAACCCGAAAACTCAATTTTCTCTTTTATCGTCCCCCGCGTTATTC 5 0.125 No Hit ATGAATTCCTCACTTCATCATTTCTTTCATTATTTTTTCTTCCTGATGAA 5 0.125 No Hit GAAGAAGGTCGAGTCCAAGAACTCTTTGGAGAACTATGCCTACAACATGA 5 0.125 No Hit ACCAAATCTGCTGCCAAGAAGGGTGGCAAGTGAACGGAGCAGGTTGATGC 5 0.125 No Hit ATCTACCTGTGGAGGAAGCAGCAAGAGAAAGAGTGAAGAGAGGAGATTTA 5 0.125 No Hit GCAAGTCACTGTACAAGAGACACTGGAAAAGCATGAGCTCACAAAACCAC 5 0.125 No Hit CCTGTCTGTTATCATGAAGTCACACTCAGTTTCATCCTCACCTTCATCTT 5 0.125 No Hit GATGGTTTCTTGCGAGTACAGTGAGATGCAAGAACCAGTTGAGATTCCCG 5 0.125 No Hit TGGATATCCTATATACTCAGCCTCTAAAGGAGCAATTAACCAACTCACAA 5 0.125 No Hit TGGCAACAAAGAATTGGTAGCTTTCAAGACAACTAGAAGAAAGAGAAAGT 5 0.125 No Hit GGATAGACAGTCCTTTTTTTTCCCACAAGCAGACCAACCACTTCAAGCAT 5 0.125 No Hit GCCACAAGAGTAACAGCAGCTCATTATGATGTCAAACTCAGCCCATCATT 5 0.125 No Hit AAGTAGAAGTGAAATGGCAATGGAAGATTACTGTGGAGACGAATTGGAAC 5 0.125 No Hit GAATTTTGGGGAGAGTAAGAGGAGTCCTTCCAGCGCAAATGGGAATTTCT 5 0.125 No Hit GCTTGAGATGGATGAGGAATATGAGGGCAATGTTGAGGCCACTGGCGAGG 5 0.125 No Hit TTAGAAACCATTCCAATTTCCCATCAACACCATCACCATCACCTACCGCT 5 0.125 No Hit GGTGAAAAACATGTTTCCAGGGGATTAGGTCGTTTTGTTTGCTGCAAGGC 5 0.125 No Hit GGAATAGCTATGAGGTGAACGTCACCATCGTTGATGTGGACTTCTCTTCC 5 0.125 No Hit ATCGACTGCTGGGCGTATTTCACATTCAAGGAAATGAATTCATCCAGGAT 5 0.125 No Hit GGAGAAGGCATAAGCTAGCAATGAAGTTCTTGTTCCAGTGCCCTTGCTGC 5 0.125 No Hit TGGTGGTGGAGGTGGTGCTGGTGGCGGACTTGGCTCTCTTGATTTCCTTA 5 0.125 No Hit CCATCATCTTGCCTCCTCTTGGTGTCTTCCTCAGGTTTGGCTGCGGGGTG 5 0.125 No Hit GTTCATCTCACGACCTTGGATGGGAGGACCCTCACCATCCCCGTCAACAA 5 0.125 No Hit AATAAGGTCATGCCAGATGGTGTGAATGCCAAGCTTCTTGGATGCCATGG 5 0.125 No Hit GTATAATCCAAAGCAAGGGCTTGATTCTCTAGTTATAACGCCAATTTCAC 5 0.125 No Hit TGACAAATGATGAGATTGCGGCTGATCTCAAGGAGCATGTCATCAAGCCC 5 0.125 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0 0.0 0.0 0.0 0.0 64-65 0.0 0.0 0.0 0.0 0.0 66-67 0.0375 0.0 0.0 0.0 0.0 68-69 0.0875 0.0 0.0 0.0 0.0 70-71 0.1125 0.0 0.0 0.0 0.0 72-73 0.1875 0.0 0.0 0.0 0.0 74-75 0.25 0.0 0.0 0.0 0.0 76-77 0.275 0.0 0.0 0.0 0.0 78-79 0.3875 0.0 0.0 0.0 0.0 80-81 0.425 0.0 0.0 0.0 0.0 82-83 0.5125 0.0 0.0 0.0 0.0 84-85 0.675 0.0 0.0 0.0 0.0 86-87 0.825 0.0 0.0 0.0 0.0 88-89 0.9875 0.0 0.0 0.0 0.0 90-91 1.2000000000000002 0.0 0.0 0.0 0.0 92-93 1.35 0.0 0.0 0.0 0.0 94-95 1.6375000000000002 0.0 0.0 0.0 0.0 96-97 1.8125 0.0 0.0 0.0 0.0 98-99 2.05 0.0 0.0 0.0 0.0 100-101 2.3 0.0 0.0 0.0 0.0 102-103 2.4625 0.0 0.0 0.0 0.0 104-105 2.7375 0.0 0.0 0.0 0.0 106-107 3.2375 0.0 0.0 0.0 0.0 108-109 3.6125 0.0 0.0 0.0 0.0 110-111 3.975 0.0 0.0 0.0 0.0 112-113 4.4375 0.0 0.0 0.0 0.0 114-115 4.949999999999999 0.0 0.0 0.0 0.0 116-117 5.3375 0.0 0.0 0.0 0.0 118-119 5.6875 0.0 0.0 0.0 0.0 120-121 6.0375 0.0 0.0 0.0 0.0 122-123 6.625 0.0 0.0 0.0 0.0 124-125 7.0875 0.0 0.0 0.0 0.0 126-127 7.675000000000001 0.0 0.0 0.0 0.0 128-129 8.837499999999999 0.0 0.0 0.0 0.0 130-131 9.8375 0.0 0.0 0.0 0.0 132-133 10.6 0.0 0.0 0.0 0.0 134-135 11.2875 0.0 0.0 0.0 0.0 136-137 11.9125 0.0 0.0 0.0 0.0 138-139 12.5625 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CAAGACC 10 0.006830828 145.0 4 AAGACCA 10 0.006830828 145.0 5 AGACCAA 10 0.006830828 145.0 6 >>END_MODULE Read 2429751 spots for SRR26075349.sra Written 2429751 spots for SRR26075349.sra Read 2429751 spots for SRR26075349.sra Written 2429751 spots for SRR26075349.sra Read 2429765 spots for SRR26075349.sra Written 2429765 spots for SRR26075349.sra Read 2429751 spots for SRR26075349.sra Written 2429751 spots for SRR26075349.sra Read 2429751 spots for SRR26075349.sra Written 2429751 spots for SRR26075349.sra Read 2429751 spots for SRR26075349.sra Written 2429751 spots for SRR26075349.sra Read 2429751 spots for SRR26075349.sra Written 2429751 spots for SRR26075349.sra Read 2429751 spots for SRR26075349.sra Written 2429751 spots for SRR26075349.sra Read 2429751 spots for SRR26075349.sra Written 2429751 spots for SRR26075349.sra Read 2429751 spots for SRR26075349.sra Written 2429751 spots for SRR26075349.sra Read 2429751 spots for SRR26075349.sra Written 2429751 spots for SRR26075349.sra Read 2429751 spots for SRR26075349.sra Written 2429751 spots for SRR26075349.sra Read 2429751 spots for SRR26075349.sra Written 2429751 spots for SRR26075349.sra Read 2429751 spots for SRR26075349.sra Written 2429751 spots for SRR26075349.sra Read 2429751 spots for SRR26075349.sra Written 2429751 spots for SRR26075349.sra Read 2429751 spots for SRR26075349.sra Written 2429751 spots for SRR26075349.sra Read 2429751 spots for SRR26075349.sra Written 2429751 spots for SRR26075349.sra Read 2429751 spots for SRR26075349.sra Written 2429751 spots for SRR26075349.sra Read 2429751 spots for SRR26075349.sra Written 2429751 spots for SRR26075349.sra Read 2429751 spots for SRR26075349.sra Written 2429751 spots for SRR26075349.sra SRR ids: ['SRR26075349.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_n24o9wl4 SRR26075349.sra spots: 48595034 blocks: [[1, 2429751], [2429752, 4859502], [4859503, 7289253], [7289254, 9719004], [9719005, 12148755], [12148756, 14578506], [14578507, 17008257], [17008258, 19438008], [19438009, 21867759], [21867760, 24297510], [24297511, 26727261], [26727262, 29157012], [29157013, 31586763], [31586764, 34016514], [34016515, 36446265], [36446266, 38876016], [38876017, 41305767], [41305768, 43735518], [43735519, 46165269], [46165270, 48595034]] SRR26075349 file size 17949633 SRR26075349 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o SRR26075349 SRR26075349_1.fastq SRR26075349_2.fastq Input file: SRR26075349_1.fastq Paired file: SRR26075349_2.fastq trimmed: SRR26075349-trimmed-pair1.fastq, SRR26075349-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Wed Feb 12 02:49:07 2025 >> started Wed Feb 12 02:50:05 2025 >> done (57.373s) 48595034 read pairs processed; of these: 251 ( 0.00%) short read pairs filtered out after trimming by size control 319982 ( 0.66%) empty read pairs filtered out after trimming by size control 48274801 (99.34%) read pairs available; of these: 8578194 (17.77%) trimmed read pairs available after processing 39696607 (82.23%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 18 19 0.00% 19 33 0.00% 20 36 0.00% 21 40 0.00% 22 32 0.00% 23 51 0.00% 24 38 0.00% 25 56 0.00% 26 59 0.00% 27 59 0.00% 28 63 0.00% 29 72 0.00% 30 61 0.00% 31 48 0.00% 32 68 0.00% 33 68 0.00% 34 79 0.00% 35 74 0.00% 36 96 0.00% 37 79 0.00% 38 86 0.00% 39 108 0.00% 40 107 0.00% 41 107 0.00% 42 145 0.00% 43 150 0.00% 44 177 0.00% 45 147 0.00% 46 165 0.00% 47 150 0.00% 48 227 0.00% 49 208 0.00% 50 305 0.00% 51 304 0.00% 52 349 0.00% 53 377 0.00% 54 432 0.00% 55 461 0.00% 56 533 0.00% 57 602 0.00% 58 669 0.00% 59 793 0.00% 60 994 0.00% 61 1167 0.00% 62 1256 0.00% 63 1361 0.00% 64 1665 0.00% 65 1745 0.00% 66 2043 0.00% 67 2002 0.00% 68 2525 0.01% 69 2699 0.01% 70 3397 0.01% 71 4029 0.01% 72 4582 0.01% 73 5047 0.01% 74 5882 0.01% 75 6763 0.01% 76 7686 0.02% 77 8416 0.02% 78 9263 0.02% 79 10722 0.02% 80 11763 0.02% 81 13378 0.03% 82 15266 0.03% 83 17081 0.04% 84 19157 0.04% 85 21801 0.05% 86 23288 0.05% 87 25117 0.05% 88 26954 0.06% 89 28631 0.06% 90 31280 0.06% 91 34843 0.07% 92 37238 0.08% 93 40644 0.08% 94 44019 0.09% 95 48196 0.10% 96 51890 0.11% 97 54749 0.11% 98 57266 0.12% 99 60299 0.12% 100 63689 0.13% 101 66511 0.14% 102 70856 0.15% 103 73945 0.15% 104 78479 0.16% 105 84151 0.17% 106 88505 0.18% 107 90495 0.19% 108 96376 0.20% 109 98477 0.20% 110 98330 0.20% 111 102320 0.21% 112 106477 0.22% 113 111272 0.23% 114 115418 0.24% 115 121350 0.25% 116 123446 0.26% 117 128395 0.27% 118 133588 0.28% 119 134247 0.28% 120 136393 0.28% 121 140795 0.29% 122 142260 0.29% 123 148016 0.31% 124 152444 0.32% 125 156443 0.32% 126 160290 0.33% 127 167185 0.35% 128 165793 0.34% 129 172576 0.36% 130 176098 0.36% 131 176343 0.37% 132 181639 0.38% 133 182080 0.38% 134 185231 0.38% 135 191972 0.40% 136 193667 0.40% 137 195108 0.40% 138 200678 0.42% 139 207022 0.43% 140 204537 0.42% 141 206734 0.43% 142 212094 0.44% 143 210843 0.44% 144 214384 0.44% 145 219919 0.46% 146 221677 0.46% 147 225764 0.47% 148 227427 0.47% 149 230894 0.48% 150 231724 0.48% 151 39696607 82.23% 48274801 reads passed initial QC criterion=sequence-density sequence-density=1.46 sequence-density-rank=1 fanout-score=2.69 fanout-score-rank=29 prefix-density=1.53 prefix-fanout=2.6 sequence=GCATTCTCAGGCAGCCTAAACCTCCTC criterion=fanout-score sequence-density=0.23 sequence-density-rank=35 fanout-score=20.75 fanout-score-rank=1 prefix-density=1.13 prefix-fanout=4.3 sequence=CTTCCTCTTTTTTAAGCCCTGGAAGATC criterion=sequence-density sequence-density=1.03 sequence-density-rank=1 fanout-score=3.22 fanout-score-rank=30 prefix-density=1.04 prefix-fanout=3.2 sequence=CTGCCTGAGAATGC criterion=fanout-score sequence-density=0.02 sequence-density-rank=42 fanout-score=241.89 fanout-score-rank=1 prefix-density=0.37 prefix-fanout=13.9 sequence=AAGAGAAGAAACACTAAAAAAATCACACAAGCGAAATCTCCAGCGAGAAGAGAAAGATGGATTTCAGAATCATGGGTCTTGACGCGCCACTCTTCAACACCCTCCAGCACATGATGGATGCAAGTGATCATGAGGCAGACAAGTCCTTCAATGCGCCAACACGCACTTACGTACGTGATGCCAAGGCAATGGCATCAACACCAGCTGATGTGAAAGAGTATCCAA SRR26075349 testing PE reads STAR mapping to Ensembl genome Started job on | Feb 12 02:51:44 Started mapping on | Feb 12 02:51:44 Finished on | Feb 12 02:59:26 Mapping speed, Million of reads per hour | 376.17 Number of input reads | 48274801 Average input read length | 289 UNIQUE READS: Uniquely mapped reads number | 36702286 Uniquely mapped reads % | 76.03% Average mapped length | 290.82 Number of splices: Total | 25072959 Number of splices: Annotated (sjdb) | 24226076 Number of splices: GT/AG | 24653851 Number of splices: GC/AG | 278668 Number of splices: AT/AC | 28764 Number of splices: Non-canonical | 111676 Mismatch rate per base, % | 0.48% Deletion rate per base | 0.04% Deletion average length | 3.17 Insertion rate per base | 0.03% Insertion average length | 3.11 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 1621681 % of reads mapped to multiple loci | 3.36% Number of reads mapped to too many loci | 109410 % of reads mapped to too many loci | 0.23% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 19.87% % of reads unmapped: other | 0.51% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 9950834 9950834 9950834 N_multimapping 1621681 1621681 1621681 N_noFeature 827450 36312882 1083970 N_ambiguous 896008 7669 757069 UnstrandedReadsAssigned:34978828 PositiveStrandReadsAssigned:381735 NegativeStrandReadsAssigned:34861247 Dataset is classified negative stranded MeadianReadLen=147 20thPercentileLength=147 echo kmer=143 SRR26075349 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,400 [index] number of k-mers: 62,057,036 [index] number of equivalence classes: 130,681 [quant] running in paired-end mode [quant] will process pair 1: SRR26075349-trimmed-pair1.fastq SRR26075349-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 48,274,801 reads, 41,485,032 reads pseudoaligned [quant] estimated average fragment length: 201.777 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,195 rounds 52401 SRR26075349.ke.tsv 34699 SRR26075349.se.tsv 87100 total ==> SRR26075349.ke.tsv <== target_id length eff_length est_counts tpm Potri.005G200100.1.v4.1 2018 1817.22 9252 107.433 Potri.005G024800.1.v4.1 1035 834.223 14492 366.569 Potri.004G059700.1.v4.1 961 760.233 107 2.96993 Potri.007G009000.2.v4.1 1416 1215.22 0 0 Potri.003G141000.2.v4.1 2943 2742.22 1173.32 9.02864 Potri.016G087400.1.v4.1 270 96.1674 2586 567.427 Potri.015G069301.1.v4.1 564 364.323 0 0 Potri.010G195200.1.v4.1 1773 1572.22 82 1.10055 Potri.012G127500.1.v4.1 977 776.223 6225 169.224 ==> SRR26075349.se.tsv <== Potri.001G166300.v4.1 0 Potri.001G448400.v4.1 37 Potri.001G233950.v4.1 0 Potri.001G122700.v4.1 107 Potri.001G212900.v4.1 0 Potri.001G182400.v4.1 0 Potri.001G256600.v4.1 0 Potri.001G040500.v4.1 5 Potri.001G416900.v4.1 0 Potri.001G452600.v4.1 305 SRR26075349 completed mapping pipeline successfully