Starting /dee2/code/volunteer_pipeline.sh SRR26075350 current disk space = 3052789112832 free memory = 1422706040 SRR26075350 SRAfilesize 20120d6a22ed349fa74f78640e1e6fbf SRR26075350.sra SRR26075350.sra file validated SRR26075350 is paired end SRR26075350 is conventional basespace SRR26075350 read1 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR26075350_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.53025 37.0 37.0 37.0 37.0 37.0 2 36.5725 37.0 37.0 37.0 37.0 37.0 3 36.65 37.0 37.0 37.0 37.0 37.0 4 36.633 37.0 37.0 37.0 37.0 37.0 5 36.714 37.0 37.0 37.0 37.0 37.0 6 36.74 37.0 37.0 37.0 37.0 37.0 7 36.6285 37.0 37.0 37.0 37.0 37.0 8 36.6685 37.0 37.0 37.0 37.0 37.0 9 36.672 37.0 37.0 37.0 37.0 37.0 10-14 36.665 37.0 37.0 37.0 37.0 37.0 15-19 36.624199999999995 37.0 37.0 37.0 37.0 37.0 20-24 36.6065 37.0 37.0 37.0 37.0 37.0 25-29 36.5357 37.0 37.0 37.0 37.0 37.0 30-34 36.464200000000005 37.0 37.0 37.0 37.0 37.0 35-39 36.3993 37.0 37.0 37.0 37.0 37.0 40-44 36.31439999999999 37.0 37.0 37.0 37.0 37.0 45-49 36.1711 37.0 37.0 37.0 37.0 37.0 50-54 36.1043 37.0 37.0 37.0 37.0 37.0 55-59 36.0924 37.0 37.0 37.0 37.0 37.0 60-64 35.9701 37.0 37.0 37.0 37.0 37.0 65-69 35.9403 37.0 37.0 37.0 37.0 37.0 70-74 36.001799999999996 37.0 37.0 37.0 37.0 37.0 75-79 36.057 37.0 37.0 37.0 37.0 37.0 80-84 36.0364 37.0 37.0 37.0 37.0 37.0 85-89 35.9274 37.0 37.0 37.0 37.0 37.0 90-94 35.9091 37.0 37.0 37.0 37.0 37.0 95-99 35.9151 37.0 37.0 37.0 37.0 37.0 100-104 35.87480000000001 37.0 37.0 37.0 37.0 37.0 105-109 35.764500000000005 37.0 37.0 37.0 37.0 37.0 110-114 35.597899999999996 37.0 37.0 37.0 37.0 37.0 115-119 35.605599999999995 37.0 37.0 37.0 37.0 37.0 120-124 35.5862 37.0 37.0 37.0 37.0 37.0 125-129 35.4904 37.0 37.0 37.0 37.0 37.0 130-134 35.328199999999995 37.0 37.0 37.0 32.2 37.0 135-139 35.269800000000004 37.0 37.0 37.0 32.2 37.0 140-144 35.0865 37.0 37.0 37.0 25.0 37.0 145-149 34.994299999999996 37.0 37.0 37.0 25.0 37.0 150-151 34.714 37.0 37.0 37.0 25.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.0 1101 2 0.0 1101 3 0.0 1101 4 0.0 1101 5 0.0 1101 6 0.0 1101 7 0.0 1101 8 0.0 1101 9 0.0 1101 10-14 0.0 1101 15-19 0.0 1101 20-24 0.0 1101 25-29 0.0 1101 30-34 0.0 1101 35-39 0.0 1101 40-44 0.0 1101 45-49 0.0 1101 50-54 0.0 1101 55-59 0.0 1101 60-64 0.0 1101 65-69 0.0 1101 70-74 0.0 1101 75-79 0.0 1101 80-84 0.0 1101 85-89 0.0 1101 90-94 0.0 1101 95-99 0.0 1101 100-104 0.0 1101 105-109 0.0 1101 110-114 0.0 1101 115-119 0.0 1101 120-124 0.0 1101 125-129 0.0 1101 130-134 0.0 1101 135-139 0.0 1101 140-144 0.0 1101 145-149 0.0 1101 150-151 0.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 18 1.0 19 1.0 20 7.0 21 0.0 22 3.0 23 5.0 24 4.0 25 4.0 26 7.0 27 13.0 28 23.0 29 29.0 30 31.0 31 38.0 32 73.0 33 111.0 34 149.0 35 439.0 36 2848.0 37 214.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 38.27870903177383 15.561671253440078 7.930948211158369 38.22867150362772 2 17.150000000000002 14.499999999999998 37.175000000000004 31.175000000000004 3 17.474999999999998 15.675 29.25 37.6 4 22.225 23.375 23.799999999999997 30.599999999999998 5 23.925 27.6 26.85 21.625 6 24.15 30.275000000000002 23.075000000000003 22.5 7 16.25 28.65 37.225 17.875 8 16.725 27.875 31.45 23.95 9 19.125 23.200000000000003 34.65 23.025000000000002 10-14 19.895 29.154999999999998 26.939999999999998 24.01 15-19 19.805 26.815 28.505000000000003 24.875 20-24 20.075000000000003 28.62 27.685 23.62 25-29 20.630000000000003 27.975 28.02 23.375 30-34 19.814999999999998 28.749999999999996 26.97 24.465 35-39 20.599999999999998 26.490000000000002 27.950000000000003 24.959999999999997 40-44 19.06 28.215 27.495000000000005 25.230000000000004 45-49 20.165 27.665 27.395000000000003 24.775 50-54 21.23 28.78 26.165 23.825 55-59 19.975 27.26 27.034999999999997 25.729999999999997 60-64 20.65 27.76 27.189999999999998 24.4 65-69 20.385 28.24 26.945000000000004 24.43 70-74 21.305 27.884999999999998 27.145000000000003 23.665 75-79 20.49 27.505000000000003 27.384999999999998 24.62 80-84 22.225 26.905 27.195000000000004 23.674999999999997 85-89 21.25 27.11 28.15 23.49 90-94 21.565 26.66 27.634999999999998 24.14 95-99 22.28 27.089999999999996 26.965 23.665 100-104 21.395 27.639999999999997 27.284999999999997 23.68 105-109 21.97 28.060000000000002 26.47 23.5 110-114 20.695 27.235 27.455000000000002 24.615000000000002 115-119 21.97 27.685 26.540000000000003 23.805 120-124 21.45 27.045 27.265 24.240000000000002 125-129 21.33 28.139999999999997 26.935 23.595 130-134 21.04 27.74 26.51 24.709999999999997 135-139 22.355 26.715 26.915 24.015 140-144 20.919999999999998 27.925 26.58 24.575 145-149 22.835 25.945 26.169999999999998 25.05 150-151 22.325 27.700000000000003 25.4625 24.5125 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 1.0 8 1.0 9 0.0 10 0.0 11 0.0 12 0.5 13 0.5 14 0.0 15 0.0 16 0.0 17 0.0 18 0.5 19 1.0 20 0.5 21 0.5 22 2.0 23 2.0 24 1.0 25 1.5 26 2.5 27 5.5 28 6.0 29 7.0 30 12.5 31 13.5 32 13.5 33 29.5 34 42.5 35 44.0 36 79.0 37 101.5 38 115.5 39 146.0 40 169.0 41 218.0 42 221.5 43 217.0 44 254.5 45 277.5 46 276.5 47 265.5 48 243.5 49 214.0 50 172.5 51 144.0 52 136.0 53 104.0 54 82.0 55 64.5 56 61.5 57 62.5 58 44.5 59 24.0 60 19.5 61 17.0 62 7.0 63 7.5 64 9.5 65 8.5 66 7.0 67 8.0 68 8.5 69 5.0 70 3.0 71 6.5 72 5.5 73 2.5 74 1.0 75 0.0 76 0.0 77 0.0 78 0.5 79 0.5 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.075 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150-151 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 60.75000000000001 #Duplication Level Percentage of deduplicated Percentage of total 1 63.12757201646091 38.35 2 21.728395061728396 26.400000000000002 3 8.518518518518519 15.525 4 3.3333333333333335 8.1 5 1.9753086419753085 6.0 6 0.7818930041152263 2.85 7 0.12345679012345678 0.525 8 0.1646090534979424 0.8 9 0.12345679012345678 0.675 >10 0.12345679012345678 0.775 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GATCGGAAGAGCACACGTCTGAACTCCAGTCACTATTCGCCATCTCGTTT 11 0.27499999999999997 TruSeq Adapter, Index 9 (97% over 36bp) GGCAGCAAGTCTAATATCTGTGGCCTTTTTCCCCTCCAAGCTCCTGGACA 10 0.25 No Hit ACTCGGTAAAGTTACTGAAAGGCAATGGAGTGAAACCAGACTGTAAAAAG 10 0.25 No Hit GCAACCCCAAGCATAAACCAACACACATCAGCCGCCACGAGGAGAGGGAA 9 0.22499999999999998 No Hit ACCGGGACTTCAGGATGGAGTTTTTCGTATTCAATGGTCAGTTTTGCCAA 9 0.22499999999999998 No Hit GGAATGTGGTTCAGTCCAAATAAATTCCGCATTTACCAAAATAACTCCTG 9 0.22499999999999998 No Hit GCTGAGGGTGGCGGCGGTGATGTCCCTGGTGAGGTGGTGTTGCCTGCAGC 8 0.2 No Hit CTTTCATAATCCAGAAGAATAATACTGTCATTAGTGCCAAGTAACCTCCA 8 0.2 No Hit ATGCGTTAACACAAAGTTTGAATCTCTCTTCGGCAGCTGCCTGTGAAGCA 8 0.2 No Hit GCTTTCTGGCCAACCGTGATCTTGCAGTGTGCAAACTCCATCGTACTTTT 8 0.2 No Hit ATCCTCCACGACCAGTTGGATCCTCATTATAGCCTCCACGGCCATTAGGA 7 0.17500000000000002 No Hit CTCTGACAACACCACTGTTTCCATGAGGCCTTGTGACCTTGCCCCAAATG 7 0.17500000000000002 No Hit GTTCGTGTACCCTGTCTCGGTTCTCCTCCCACCACAACCTTGCACGTGTT 7 0.17500000000000002 No Hit CTTCGACCTCAAGGCTATAGCTATAATTCCGAGCCTCAGCCTCATCACCC 6 0.15 No Hit CCTTTAAAGTATCTTGAAGCAACACGAACATTGTATTCTTTCCCAGGGTG 6 0.15 No Hit CCCAACAGCTCTGCCCATGATGTGATGCCGCTGAAGCCAGAGCTGCCCTT 6 0.15 No Hit ATCTGTCCTGACCTTCCCATCAACAAGGACATGTCTCTGCATCAAAATAG 6 0.15 No Hit GCCATCTACCGTACCAGATTGTGTGGACCGTTATGTTCCCAGTTAATACT 6 0.15 No Hit CCGGATTCGATGCCACCGCCGCCACAAAGCCGGCAGCAAAACTCGCCGTC 6 0.15 No Hit CTTCCTATCCCACTTGACCAAAACTGAGCAACAGCTAATTCCATTGTCTG 6 0.15 No Hit CACTGCATTAGACGCTAATGCCAATATGGCAACTCCTGAATTTGTATAAA 6 0.15 No Hit CCTCAACTTGTTTAGCCACTCAACCTTATCGCCCATGCTCTCAGCTTTTA 6 0.15 No Hit GGCCCAAAACAAGGTCTTAAAATCATAATAGCAAACTACAAATAAAAAAC 6 0.15 No Hit GCTGTACTGATCAACTAGAAGGTATTTCACATCTTCTTCACTACATTTCA 6 0.15 No Hit GTACCTCACTGAAGTAGCCAAATCCACCCTGAAAGACGTCGTCGTCGACC 6 0.15 No Hit GCCTTGCCATGATCTAGAGCAACCTTCAGAACTGATTCATTTGTTGCATT 6 0.15 No Hit CATATCTGCAATCATCATCTGGCAATGAAGCAGCAAGATCGTCATAGCCT 6 0.15 No Hit GCGCTGTAAACAAGCTTGGAAGAACCGTGTTGTTATCTTTCTTGAGGTAC 6 0.15 No Hit GATCGGAAGAGCACACGTCTGAACTCCAGTCACTATTCGCCATCTCGTAT 6 0.15 TruSeq Adapter, Index 9 (97% over 36bp) AATTGAACCTAATCTACAATTCTAAAAATTCAGTTTTACAAAGAAACCAC 6 0.15 No Hit AGCTTCCTGTCTCATCCGGCTATATCGTTGAGCAAGATGGCGGGCATCTT 6 0.15 No Hit GTGGAATTGGACGAGGAAACTATACAGGAAGAACCGCCTAAAAGGGACTG 6 0.15 No Hit AGACAACTGCAGGGGTTTTGGCAATATTGAGTTGGAAGTGAATGAGGCCA 5 0.125 No Hit GCTAGCAAGAGTCAAGTCGAATGCTGTTAAAGGAAGTGAGTTTTAGAAAA 5 0.125 No Hit GTGGTCATAAGTCCCTCAACAATGCCAAATCTGTCATGAATGACCTTAGC 5 0.125 No Hit CACCCTTCTTATTCTGCCCTGGACTGGCCAGCACGGGATGACAAGATGAT 5 0.125 No Hit ACACGGGCGGCAAAGAAAGCTTCAAGCCTGATAGCTAGAAAGTGAGGATA 5 0.125 No Hit GAAGACAATAACGGATCAGGTGCGGAATTGGACGAGGAAACTATACAGGA 5 0.125 No Hit GTGGTGGTGGCGCCGCCTCCGACGTTGGTGGTGGCTGAGGGGGGAAGTTG 5 0.125 No Hit GCTCCATTGCCTGGACAAGCAGCGAGGAGTCATCAGTCAGATCAGAATAT 5 0.125 No Hit CGGCTTTAAGCATCCCAAGAACACCATCTCTATTTCTGAGGAGCTTCCAG 5 0.125 No Hit CTCGTTGTAGCCTCCTCGACCATTAGGATTCTCGTTGTAGCCTCCTCGAC 5 0.125 No Hit CACGTGAGCTGGAAACCAAGATAGAGCTTATGGTTTGATTCTTCTTAGCA 5 0.125 No Hit CCAGTGTTCACTTGGAGACAGCAGCAAACAAGGGAGTGGTAAAGAATTTA 5 0.125 No Hit CTTCAGTGTGTGAAAGATGTTAAGGTTTCTAGAGACCTCGATGGCAACTC 5 0.125 No Hit CCTCTCATTGCCTTCTCATTGCCGAAGGTCACAAATCCAAAACCACGAGA 5 0.125 No Hit GCTATGGGAATATTGACCGCCATGATCAAAGTTTTCCGCTGTAGGCAAAG 5 0.125 No Hit GCTTCCTTGGTAATGGTCCGGCCACTTGGGCCAATCGCTACAGCTGCAGG 5 0.125 No Hit CGCGTCTGACATGTCAATCTAATAGGGGAAATTTTCTTTAGTTTCCCGGG 5 0.125 No Hit GTCACTCTGGCCCTCCTTCATCCTTGTCACATAATCCTTCAGGCTGGTCA 5 0.125 No Hit CCCACAACTTGTGCCTTAGAAGCAGGAGCAGCCCCATGGCCATTAGTGTT 5 0.125 No Hit ATCAACAAGCAACTTTGTCTCGCTAATTAGTAGTTATAATTAGCAGTAGT 5 0.125 No Hit ATCCGATGTAGACCTCCTTCGAAACCGAAACGACTCTCTGCTTCCAGGAA 5 0.125 No Hit GATGGATTCTGCTAGGCTGAGACTTGGTCATCCTTGCTTCCCCGTAGGGT 5 0.125 No Hit GGTGAGACAATCATGCTGACAAAGCGAGTTAATAAAAATCCTGAACCCCA 5 0.125 No Hit TTCTTGAGCTTGTTCAGTTCATCTCCTTGAATGCCCCAATCCAGTTTCCC 5 0.125 No Hit GTCGGACCTCGGCTTTCAGGGAGTACTTTAATGAACCACGCGTGAAGGCG 5 0.125 No Hit GTCGTGAATACCCAGATTAGTGCCCCAGTGTAAGCTCTTCAAGGGTTCAA 5 0.125 No Hit CTTGTCAAATGGAGGTTGCTCCTGGAGCCTAAATGTGTCAACGATGTCAA 5 0.125 No Hit AGCACGCAAAGAGAATGCCTTTAATGTGGGGACCACCATACCACCTTCCA 5 0.125 No Hit GTGCTCGATATGGTTGAGGAGATCGGCATAGTTCTTTATATGTTTCTCAA 5 0.125 No Hit TCCTTATCGACGGGTGCGTGAATAGCCTTCGCATCTTCCATGAGGATGAT 5 0.125 No Hit TCCACTCCTTAGCTGTCTCAACAGCTTCTACCTCGTTCGTTTTCCAATGC 5 0.125 No Hit GTCAAAACTTCCCACTTCAGAGATGTCACGTTCACTAGCCCAACCTAACA 5 0.125 No Hit CAATGGTCTAATGTGATCATCGGCAGGCGCTCTGTGACTTTGTTGGGTGG 5 0.125 No Hit CTCACATTCAACCAGGTGATCTTGGAAAAAGCAAACCTAGCCCCATTCCT 5 0.125 No Hit CTGGAAGGAGGCGGCCTGATCTGTACCGAACCCCACAAGCATTGCAAAGG 5 0.125 No Hit GAGGACAAGGTGAAGGGTAGACTCTTTTTGGATATTGTAGTCAGCCAGAG 5 0.125 No Hit CATGTTTTCAGAGTACACATGTAACAAGAAGAGGATCACATCAGCATCGC 5 0.125 No Hit CTCATTGTGTCTTATGGGACTCCCAGGTCCGACTGGGGAATTTGGAATGA 5 0.125 No Hit GTACTTGTCATCAATAGAGGGTTGCTGTGCCTTCCTTCCCTCTTTCATTC 5 0.125 No Hit TGGCAGGATCTACTATCTATCCATCCATCCATCCATCTCTTTCTTGGCTT 5 0.125 No Hit ACTCCGTGCTAAGGGCTTCGTCGATGTCTTTAGTCATATGAACCATAAGA 5 0.125 No Hit CTCAAGTATAGTATCTTTCACTTGAAATGGTCTTGCTGGAGAATTGTGAT 5 0.125 No Hit GTTCCGATTCTGCTACCTGACCAAATCTCTAGAAGATCCAATCTCAGTTA 5 0.125 No Hit CCTCTCTATTTGATTCACAAGTCCCAAGAGACAAGCCTATCAAATCAACA 5 0.125 No Hit CGGCACTGGCTTTTTAGTTATCCCAAGATTTGCATTATAAAATGCCTCAA 5 0.125 No Hit CCCAAATATTTCTTTCGGTAGGCTCTTCACAAACTTGTCAAAAGCAGATT 5 0.125 No Hit GCCTGGCATTGCAGCCTCAACACAATTTTCTTTGTTGTCTTTGCCTTCTT 5 0.125 No Hit CTGCGCCTTATGGAGGCGAGTAGCATGCTACTGAGAAGAGAACTGCACAA 5 0.125 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.025 0.0 0.0 0.0 0.0 54-55 0.025 0.0 0.0 0.0 0.0 56-57 0.025 0.0 0.0 0.0 0.0 58-59 0.025 0.0 0.0 0.0 0.0 60-61 0.025 0.0 0.0 0.0 0.0 62-63 0.05 0.0 0.0 0.0 0.0 64-65 0.0875 0.0 0.0 0.0 0.0 66-67 0.1 0.0 0.0 0.0 0.0 68-69 0.1 0.0 0.0 0.0 0.0 70-71 0.1 0.0 0.0 0.0 0.0 72-73 0.1125 0.0 0.0 0.0 0.0 74-75 0.125 0.0 0.0 0.0 0.0 76-77 0.1375 0.0 0.0 0.0 0.0 78-79 0.21250000000000002 0.0 0.0 0.0 0.0 80-81 0.225 0.0 0.0 0.0 0.0 82-83 0.225 0.0 0.0 0.0 0.0 84-85 0.225 0.0 0.0 0.0 0.0 86-87 0.2625 0.0 0.0 0.0 0.0 88-89 0.4375 0.0 0.0 0.0 0.0 90-91 0.5625 0.0 0.0 0.0 0.0 92-93 0.75 0.0 0.0 0.0 0.0 94-95 1.0875 0.0 0.0 0.0 0.0 96-97 1.3875 0.0 0.0 0.0 0.0 98-99 1.6625 0.0 0.0 0.0 0.0 100-101 1.85 0.0 0.0 0.0 0.0 102-103 2.0375 0.0 0.0 0.0 0.0 104-105 2.2125 0.0 0.0 0.0 0.0 106-107 3.025 0.0 0.0 0.0 0.0 108-109 3.2874999999999996 0.0 0.0 0.0 0.0 110-111 3.7625 0.0 0.0 0.0 0.0 112-113 4.112500000000001 0.0 0.0 0.0 0.0 114-115 4.4 0.0 0.0 0.0 0.0 116-117 4.7625 0.0 0.0 0.0 0.0 118-119 5.300000000000001 0.0 0.0 0.0 0.0 120-121 5.675 0.0 0.0 0.0 0.0 122-123 6.1875 0.0 0.0 0.0 0.0 124-125 6.5 0.0 0.0 0.0 0.0 126-127 7.0 0.0 0.0 0.0 0.0 128-129 7.65 0.0 0.0 0.0 0.0 130-131 8.462499999999999 0.0 0.0 0.0 0.0 132-133 9.2375 0.0 0.0 0.0 0.0 134-135 10.075 0.0 0.0 0.0 0.0 136-137 10.625 0.0 0.0 0.0 0.0 138-139 11.3 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AAGTATA 10 0.006830828 145.0 4 TCAAGTA 10 0.006830828 145.0 2 CCAGAGC 10 0.006830828 145.0 3 GTTCAGT 10 0.006830828 145.0 9 GAATGTG 10 0.006830828 145.0 2 GTATAGT 10 0.006830828 145.0 6 ATAGTAT 10 0.006830828 145.0 8 TATAGTA 10 0.006830828 145.0 7 CTCAAGT 10 0.006830828 145.0 1 CAAGTAT 10 0.006830828 145.0 3 GTCACTA 30 1.4118372E-5 96.666664 145 ACACGTC 40 0.0076550315 36.25 145 CCCCCCC 20 0.00593511 29.0 50-54 CAGTCAC 30 0.0014437955 24.166668 140-144 ACTCCAG 30 0.0014437955 24.166668 135-139 GTCTGAA 30 0.0014437955 24.166668 130-134 CGTCTGA 30 0.0014437955 24.166668 130-134 CCAGTCA 35 0.0035366106 20.714287 140-144 GAACTCC 35 0.0035366106 20.714287 135-139 TCTGAAC 35 0.0035366106 20.714287 130-134 >>END_MODULE SRR26075350 read2 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR26075350_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.40375 37.0 37.0 37.0 37.0 37.0 2 36.318 37.0 37.0 37.0 37.0 37.0 3 36.3345 37.0 37.0 37.0 37.0 37.0 4 36.3985 37.0 37.0 37.0 37.0 37.0 5 36.4425 37.0 37.0 37.0 37.0 37.0 6 36.3395 37.0 37.0 37.0 37.0 37.0 7 36.457 37.0 37.0 37.0 37.0 37.0 8 36.4315 37.0 37.0 37.0 37.0 37.0 9 36.4195 37.0 37.0 37.0 37.0 37.0 10-14 36.312200000000004 37.0 37.0 37.0 37.0 37.0 15-19 36.216 37.0 37.0 37.0 37.0 37.0 20-24 36.2094 37.0 37.0 37.0 37.0 37.0 25-29 36.073100000000004 37.0 37.0 37.0 37.0 37.0 30-34 35.983 37.0 37.0 37.0 37.0 37.0 35-39 35.8587 37.0 37.0 37.0 37.0 37.0 40-44 35.9181 37.0 37.0 37.0 37.0 37.0 45-49 35.829 37.0 37.0 37.0 37.0 37.0 50-54 35.6882 37.0 37.0 37.0 37.0 37.0 55-59 35.794599999999996 37.0 37.0 37.0 37.0 37.0 60-64 35.795100000000005 37.0 37.0 37.0 37.0 37.0 65-69 35.709500000000006 37.0 37.0 37.0 37.0 37.0 70-74 35.664699999999996 37.0 37.0 37.0 37.0 37.0 75-79 35.55309999999999 37.0 37.0 37.0 37.0 37.0 80-84 35.6194 37.0 37.0 37.0 37.0 37.0 85-89 35.692299999999996 37.0 37.0 37.0 37.0 37.0 90-94 35.6256 37.0 37.0 37.0 37.0 37.0 95-99 35.63869999999999 37.0 37.0 37.0 37.0 37.0 100-104 35.539699999999996 37.0 37.0 37.0 37.0 37.0 105-109 35.6124 37.0 37.0 37.0 37.0 37.0 110-114 35.48819999999999 37.0 37.0 37.0 37.0 37.0 115-119 35.4071 37.0 37.0 37.0 37.0 37.0 120-124 35.3229 37.0 37.0 37.0 34.6 37.0 125-129 35.3846 37.0 37.0 37.0 37.0 37.0 130-134 35.33030000000001 37.0 37.0 37.0 34.6 37.0 135-139 35.24515 37.0 37.0 37.0 32.2 37.0 140-144 35.198 37.0 37.0 37.0 27.4 37.0 145-149 35.0961 37.0 37.0 37.0 25.0 37.0 150-151 34.8125 37.0 37.0 37.0 25.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.0 1101 2 0.0 1101 3 0.0 1101 4 0.0 1101 5 0.0 1101 6 0.0 1101 7 0.0 1101 8 0.0 1101 9 0.0 1101 10-14 0.0 1101 15-19 0.0 1101 20-24 0.0 1101 25-29 0.0 1101 30-34 0.0 1101 35-39 0.0 1101 40-44 0.0 1101 45-49 0.0 1101 50-54 0.0 1101 55-59 0.0 1101 60-64 0.0 1101 65-69 0.0 1101 70-74 0.0 1101 75-79 0.0 1101 80-84 0.0 1101 85-89 0.0 1101 90-94 0.0 1101 95-99 0.0 1101 100-104 0.0 1101 105-109 0.0 1101 110-114 0.0 1101 115-119 0.0 1101 120-124 0.0 1101 125-129 0.0 1101 130-134 0.0 1101 135-139 0.0 1101 140-144 0.0 1101 145-149 0.0 1101 150-151 0.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 12 1.0 13 4.0 14 15.0 15 5.0 16 3.0 17 4.0 18 1.0 19 1.0 20 2.0 21 6.0 22 8.0 23 9.0 24 13.0 25 13.0 26 11.0 27 17.0 28 24.0 29 15.0 30 25.0 31 33.0 32 37.0 33 83.0 34 175.0 35 592.0 36 2653.0 37 250.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 41.83545886471618 21.980495123780948 11.70292573143286 24.48112028007002 2 30.575000000000003 25.25 25.95 18.224999999999998 3 23.125 27.775 30.2 18.9 4 27.900000000000002 33.25 21.55 17.299999999999997 5 28.325 34.55 21.525 15.6 6 24.0 37.375 20.75 17.875 7 24.25 21.475 36.025 18.25 8 22.575 26.974999999999998 27.025 23.425 9 22.275 25.374999999999996 29.799999999999997 22.55 10-14 25.885 28.115000000000002 25.465 20.535 15-19 25.040000000000003 27.735 27.089999999999996 20.135 20-24 25.53 27.775 25.56 21.135 25-29 25.330000000000002 28.33 25.56 20.78 30-34 25.025 27.865000000000002 26.369999999999997 20.74 35-39 25.755 27.650000000000002 25.775 20.82 40-44 25.679999999999996 28.044999999999998 25.94 20.335 45-49 25.019999999999996 27.900000000000002 26.43 20.65 50-54 23.82 26.91 27.47 21.8 55-59 24.685000000000002 28.53 26.150000000000002 20.635 60-64 25.295 28.01 25.71 20.985 65-69 25.224999999999998 27.675 26.08 21.02 70-74 25.335 28.03 26.775 19.86 75-79 25.21 28.83 25.525 20.435 80-84 24.5 27.57 27.0 20.93 85-89 25.025 27.67 26.424999999999997 20.880000000000003 90-94 25.405 27.04 27.07 20.485 95-99 24.89 27.884999999999998 26.685 20.54 100-104 25.435000000000002 27.29 26.540000000000003 20.735 105-109 25.605 27.68 26.82 19.895 110-114 26.195 27.83 25.629999999999995 20.345 115-119 26.435 27.685 26.5 19.38 120-124 26.145000000000003 28.1 26.39 19.365 125-129 25.845000000000002 27.92 25.94 20.294999999999998 130-134 26.75 27.705000000000002 25.759999999999998 19.785 135-139 26.50132506625331 28.056402820141006 26.09630481524076 19.34596729836492 140-144 26.960392078415683 27.510502100420087 26.255251050210042 19.27385477095419 145-149 28.141256502601042 26.58063225290116 26.17046818727491 19.10764305722289 150-151 28.732183045761438 27.68192048012003 26.03150787696924 17.554388597149288 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.5 6 0.5 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.5 14 1.0 15 0.5 16 0.0 17 1.5 18 2.0 19 1.5 20 1.0 21 0.5 22 1.0 23 0.5 24 0.5 25 0.5 26 1.5 27 2.0 28 3.5 29 6.0 30 4.5 31 13.0 32 21.0 33 21.0 34 26.5 35 27.5 36 43.0 37 68.5 38 106.0 39 144.5 40 189.0 41 227.0 42 244.0 43 264.0 44 270.0 45 271.5 46 274.5 47 276.5 48 259.0 49 214.5 50 176.5 51 157.0 52 126.0 53 97.5 54 71.5 55 60.0 56 58.5 57 49.0 58 35.0 59 23.0 60 20.5 61 16.0 62 15.0 63 15.0 64 12.5 65 5.5 66 2.5 67 3.5 68 3.0 69 2.0 70 2.5 71 1.5 72 0.0 73 1.5 74 1.5 75 1.0 76 1.0 77 0.0 78 0.0 79 0.5 80 1.5 81 1.0 82 0.0 83 0.0 84 1.0 85 2.5 86 2.5 87 3.0 88 2.5 89 3.0 90 3.5 91 1.0 92 1.5 93 2.5 94 3.0 95 4.0 96 2.5 97 0.5 98 0.5 99 0.5 100 7.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.025 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.005 140-144 0.02 145-149 0.04 150-151 0.025 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 61.824999999999996 #Duplication Level Percentage of deduplicated Percentage of total 1 65.02224019409624 40.2 2 20.703598867771937 25.6 3 7.925596441568944 14.7 4 3.194500606550748 7.9 5 1.8196522442377678 5.625 6 0.7682976142337242 2.85 7 0.16174686615446826 0.7000000000000001 8 0.16174686615446826 0.8 9 0.16174686615446826 0.8999999999999999 >10 0.08087343307723413 0.7250000000000001 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG 19 0.475 No Hit GGAATCACTGGATGCCGTGAATGTGAACCTAGATGCATTGCAAAAGATTG 10 0.25 No Hit AAGGTCTATAGGCCCGTCTGGCAGCTTACACCAAAAGGCTCGGGCTGCTT 9 0.22499999999999998 No Hit AACGAGCGATGGAGCGGCAATGTCCAGAATTACCATCGTTTTGTGGCTCG 9 0.22499999999999998 No Hit CGGGGGTGAAGAGAGAAGTGCCTGTGCCTGTGTTTCAAAGCGTTGGCTTA 9 0.22499999999999998 No Hit GAAGAATGTAATTCTTTTACATTGCCCGGAGTGTGATACCTACTTGGATC 9 0.22499999999999998 No Hit AGAAGCTGTATATTCCAAGGAGATTAATGATTGGGAAAGATGAATATGTT 8 0.2 No Hit GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGT 8 0.2 No Hit CACGATGTGGCCACTGTCTCAAAGAGTGATTATGATAATTGCAACACAGG 8 0.2 No Hit TCTGGATTTGAGTGGAGAGAGCCTCCAAAGTGGAAGGACCAAAGATACAA 8 0.2 No Hit GTTTGGCTTCATGTTTATTGCCCTGATATGGAAGTTTGACTTCGCTCCAT 7 0.17500000000000002 No Hit GCTAAAACTGAGACATCATCAATAGATGGTTCTGTGAGGACTAGTATGTC 7 0.17500000000000002 No Hit AAACCTTTTTGCGTGTAGTGTAAATTTAGCATGGCAAAATCAGCCTTCTC 7 0.17500000000000002 No Hit GTCAACACCAAGGAAGAAGTTGCATGGTATGCTGGTAAGCGCATGGCATA 7 0.17500000000000002 No Hit GCTGACATCACTTTTTATGTGGCTCAGCTTACGTACTTCATCACCTGATT 6 0.15 No Hit CAAGCTGACGGGCGTTTACCCGTGTCCCAAAGGCGGAACTATAAAAGCGT 6 0.15 No Hit GATGATCACAAGGTGGACATGCACATAGGATGCACTTTCAACCATCGCTA 6 0.15 No Hit GCTTGGTTACACAAAAAAAGCAAGAAGAAAGACAATTCCGTGGTGTTATT 6 0.15 No Hit AGTTGGAACCAGTGGCGATGATCAAGCTGCTCTTGCAGCACCCATGGTTG 6 0.15 No Hit GATACTACTCGACGATTTTTTGTTGACACTGTTCAGATTTGTCCCTTACA 6 0.15 No Hit GAAACATATTCAAGATGCAGCGCAAGAGGAAATCACGTAGAGGTGGACCT 6 0.15 No Hit GCAAGGTTGATTGCAAAGTACAAACCCATAACGCCTGTTATATCTGTTGT 6 0.15 No Hit CCCTCGTCTTTATCAAAAATATCGTAGGGTTTCTCAATGGCTGCTGAGGT 6 0.15 No Hit AGGCTCAAGAGAGGCGCAGCTCAGCAGAGTTACTGACCTTTTCAACTTTA 6 0.15 No Hit ATTAGATTCCCCAGTGTTCCATCGAGCAAAACCATCAATGATTCTGGCAG 6 0.15 No Hit GGCGGCGGCGTAAGCCAGAGTCAAAGTCAAAGAAGCGGTGGTGGATTTGA 6 0.15 No Hit GAAAGGAAAGGACATCACTGAGCTGATTGCTTCCGGCAGGGAAAAGTTGG 6 0.15 No Hit GAGTGTTAAAATTGTTGATCTAAGCTACCTCTCTTCCGTTATCCTCTTAA 6 0.15 No Hit CAAGAACTCATTCCATGAAATGAAATGGAGAAAAGTTCATAGGTACATAG 6 0.15 No Hit TGCAGCTATATATGGTGATGCTCGTAAACATGTGGAACAGGAGCAGGATG 6 0.15 No Hit CTTTGATATTAGGTATTACATCTACGAGCTTCTGAAGGCATTGGATTATT 6 0.15 No Hit CACCATGCCCTTGTAATATAACCCACCCTTCATAGTCCATCCCTTCTTCC 6 0.15 No Hit TATAACATTTACTTTTTTTCACAGGTCCATGAAAGGCAAGATACTGGGGA 6 0.15 No Hit ATTTTTTTTCTTTTTTTCCCTCTCTCTCACCGGCCGACAAAGGAGTTGAA 5 0.125 No Hit ACCAGCTTGAGCAAATTCAGTTTCTAAGCAAAAGCTTTCCAGGCCCCTTT 5 0.125 No Hit TATGGGTTTGATACATCTGCCGCAGTGGCAGCTGCTGTAATGAATTCAAG 5 0.125 No Hit GTCGTTTTTAACAAATATCTCCTTCTTAAGATAAAGATAAAGATAAAAAT 5 0.125 No Hit GGTCAAAAGCTACCAGATGCAGAAGATGACCAGCATTATGATCACTACGA 5 0.125 No Hit CCCCTCCCCATATATTCTCTCCCTATGTATCATTCAACCTAGTGTAATGC 5 0.125 No Hit GTTTACTTTGTGTCTTTTAAATCCCCTGGAGACTGAAAGAACTACAATTT 5 0.125 No Hit TGGGAATATATGTTGTCAGCAAGAATGTGATGCTGGATCTGCTGAGAGAA 5 0.125 No Hit AAAATGAGGGCAGCGATTGGGAAGTTTGGGTTGACAGGCAAGGCCCAGGT 5 0.125 No Hit GTGAGAGGCAAACTTTCACTGGTCCTCCTGAAAATGTAAGGGACCATGTA 5 0.125 No Hit TCTGAAAGACAAATGGAGTCCTGCTCTTCAGATACGAACCGTACTTTTGA 5 0.125 No Hit CTTCATTTGAGCCAGGCCGCCCTGGGGAATCTGCAGTGGAGTTGGGTCGA 5 0.125 No Hit CCGGCAAGGAGTCAGGCGGCGGCGGCGTGATGAGATCGCACAGTGAGGAA 5 0.125 No Hit GTGACGTTAAAGGAGAGAGATCCTTCGCTGAAGATGTTGAGCCGAGGCCT 5 0.125 No Hit CCGGAGCTGAGTATATTGTGGAGTCAACTGGAGTTTTCACTGACAAGGAA 5 0.125 No Hit GCAATGTCTGCCGAGGTTGAGTACAGGTGCTTTGTTGGCGGCCTCGCTTG 5 0.125 No Hit GTAAGAGAGAGAAAGAGGATCGATGGTTATTGTGAAGAGGAGGATCCGAT 5 0.125 No Hit AGAGTTAGAGTTCTAGAGCATTTCACACAGAAAGAATTTCTCCGGTATGG 5 0.125 No Hit GGGAGTCATGGTGATGTTGTCTTCTTAACAAATCGAGGGGAGGTGACATC 5 0.125 No Hit CAAGGCCGAGTGGGTGATTTTGGTTTCCCCAGGATGAAGAAAGCAGCAGC 5 0.125 No Hit CGCATTCGATCTCAAAGGAGGCTGCTTCTGCGGTGCAATCCGCTACACGA 5 0.125 No Hit GGTACATCCAAAGCAGTGGAGAACATTGGAAACAGAAGGATGAAGATGGG 5 0.125 No Hit TGGGTATCCGTTCAACCTTGATAGACTGAATTTCCTTAAAGAGCAAGAAG 5 0.125 No Hit AGAGAGGCCGAAAACCGAAGCCCAAAGAAGATCAACAGCAGCAGCAGCAG 5 0.125 No Hit ATGATGAATCACTGCAAACAACAGCAAGTGTATTTTAAGTTATGTTTATT 5 0.125 No Hit TTTTGGCTTGGTTTGAGGTAAGGAAGAAGGCACGAGGCTGTTGATCTGAG 5 0.125 No Hit AGAGCTCTTCCGCTTCTCCGCTACAATGGTGAACGTTCCTAAGACTAAGA 5 0.125 No Hit CCCATGCCTGTCACGGTGATGACACTTCTTGTTGCAACTTTGTTGCTCAT 5 0.125 No Hit GTGATATTTGGCTGTATCCCTGTTATTATAGCAGATGACATTGTTTTACC 5 0.125 No Hit CCTTTGACGAGTTCTATTCAGAAATGCCTTGGTTAGCCCTTCCATTTGGT 5 0.125 No Hit AGCTAAGCACACAAATTAAGGCTTAAAGATATAGAGAGAAAGAAACAACA 5 0.125 No Hit TGGTTATTTTGGGAATCCTGGCTTTGGTGTTGGTATGTCATATCCTGGAA 5 0.125 No Hit AAGAAGAGAAGCTAGATGGGGATGCAGCTTTGAACAAATTTTTCCGAGAA 5 0.125 No Hit AGCTTGAAGAAATAGTTCAAGTAGCTCTGTTATGTACCCAATACCTTCCA 5 0.125 No Hit GTGGAATCAAATACGAAGCCTTTGTTTGTAATTTTTGGGTGATGGAGTTC 5 0.125 No Hit TTTCTCTGTTGCCATTCATGGTGGCTCTGCTAACTGGGTTTTCTCTGGGA 5 0.125 No Hit CGTACGTGGGTGTGTTGCTGTACGGAGTAGGAGGCATAGTGGTAGCAGGG 5 0.125 No Hit GAATTAGAATACTATAATGATGAGGAAATATTGGATTCTTCCAGGAAACA 5 0.125 No Hit CTTGAAGCTCTCAACTTTTTGTTGACATCAGAGGTTCGTAGTCAAGATGC 5 0.125 No Hit GTACTCAGTACTCAGTCTTCAGCGCAGACCGCTCAGCGACAACTTTCCTC 5 0.125 No Hit GCAGATTTTTGTGAAGACCTTGACAGGGAAGACCATTACTCTAGAGGTAG 5 0.125 No Hit GACTTTCTGGTCATCATCTGGAGGGGTTGTTTACAGCACTGACTGTAACA 5 0.125 No Hit TAGAGTTTGGCTGGGCACTTACAACACAGCTGATGAAGCCGCTAAAGCTT 5 0.125 No Hit GCAAGAGATCGCACAGAGCTAGAGAGAGAGAGAGAAGTGAAGAGAAGAGG 5 0.125 No Hit CACTTATGATCTTGCCGAAGAAGCCGAGACGGTGGAGAAACTATTGCCTG 5 0.125 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.025 0.0 0.0 0.0 0.0 54-55 0.025 0.0 0.0 0.0 0.0 56-57 0.025 0.0 0.0 0.0 0.0 58-59 0.025 0.0 0.0 0.0 0.0 60-61 0.025 0.0 0.0 0.0 0.0 62-63 0.05 0.0 0.0 0.0 0.0 64-65 0.0875 0.0 0.0 0.0 0.0 66-67 0.1 0.0 0.0 0.0 0.0 68-69 0.1 0.0 0.0 0.0 0.0 70-71 0.1 0.0 0.0 0.0 0.0 72-73 0.1125 0.0 0.0 0.0 0.0 74-75 0.125 0.0 0.0 0.0 0.0 76-77 0.1375 0.0 0.0 0.0 0.0 78-79 0.21250000000000002 0.0 0.0 0.0 0.0 80-81 0.225 0.0 0.0 0.0 0.0 82-83 0.225 0.0 0.0 0.0 0.0 84-85 0.225 0.0 0.0 0.0 0.0 86-87 0.2625 0.0 0.0 0.0 0.0 88-89 0.4375 0.0 0.0 0.0 0.0 90-91 0.5625 0.0 0.0 0.0 0.0 92-93 0.75 0.0 0.0 0.0 0.0 94-95 1.0875 0.0 0.0 0.0 0.0 96-97 1.3875 0.0 0.0 0.0 0.0 98-99 1.6625 0.0 0.0 0.0 0.0 100-101 1.8624999999999998 0.0 0.0 0.0 0.0 102-103 2.075 0.0 0.0 0.0 0.0 104-105 2.2625 0.0 0.0 0.0 0.0 106-107 3.025 0.0 0.0 0.0 0.0 108-109 3.2874999999999996 0.0 0.0 0.0 0.0 110-111 3.7625 0.0 0.0 0.0 0.0 112-113 4.1375 0.0 0.0 0.0 0.0 114-115 4.45 0.0 0.0 0.0 0.0 116-117 4.825 0.0 0.0 0.0 0.0 118-119 5.375 0.0 0.0 0.0 0.0 120-121 5.775 0.0 0.0 0.0 0.0 122-123 6.3125 0.0 0.0 0.0 0.0 124-125 6.625 0.0 0.0 0.0 0.0 126-127 7.125 0.0 0.0 0.0 0.0 128-129 7.775 0.0 0.0 0.0 0.0 130-131 8.6125 0.0 0.0 0.0 0.0 132-133 9.412500000000001 0.0 0.0 0.0 0.0 134-135 10.2 0.0 0.0 0.0 0.0 136-137 10.7375 0.0 0.0 0.0 0.0 138-139 11.399999999999999 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CCAAAGC 10 0.006830828 145.0 8 GTACATC 10 0.006830828 145.0 2 GAATGTA 10 0.006830828 145.0 4 TACATCC 10 0.006830828 145.0 3 ACATCCA 10 0.006830828 145.0 4 GTAATTC 10 0.006830828 145.0 8 AATGTAA 10 0.006830828 145.0 5 GGTACAT 10 0.006830828 145.0 1 GTGTTAT 30 1.4118372E-5 96.666664 145 GTCGTGT 40 0.0076550315 36.25 145 GAGTGTT 30 0.0014437955 24.166668 140-144 GTGTAGG 30 0.0014437955 24.166668 130-134 AGTGTTA 30 0.0014437955 24.166668 140-144 AGAGTGT 30 0.0014437955 24.166668 140-144 TGTAGGG 30 0.0014437955 24.166668 130-134 GTAGGGA 30 0.0014437955 24.166668 130-134 GAAAGAG 45 6.5511256E-4 19.333332 135-139 AGAGCGT 40 0.0076550315 18.125 120-124 GCGTCGT 40 0.0076550315 18.125 125-129 CGTCGTG 40 0.0076550315 18.125 125-129 >>END_MODULE Read 819553 spots for SRR26075350.sra Written 819553 spots for SRR26075350.sra Read 819553 spots for SRR26075350.sra Written 819553 spots for SRR26075350.sra Read 819553 spots for SRR26075350.sra Written 819553 spots for SRR26075350.sra Read 819553 spots for SRR26075350.sra Written 819553 spots for SRR26075350.sra Read 819553 spots for SRR26075350.sra Written 819553 spots for SRR26075350.sra Read 819553 spots for SRR26075350.sra Written 819553 spots for SRR26075350.sra Read 819553 spots for SRR26075350.sra Written 819553 spots for SRR26075350.sra Read 819553 spots for SRR26075350.sra Written 819553 spots for SRR26075350.sra Read 819553 spots for SRR26075350.sra Written 819553 spots for SRR26075350.sra Read 819553 spots for SRR26075350.sra Written 819553 spots for SRR26075350.sra Read 819553 spots for SRR26075350.sra Written 819553 spots for SRR26075350.sra Read 819553 spots for SRR26075350.sra Written 819553 spots for SRR26075350.sra Read 819553 spots for SRR26075350.sra Written 819553 spots for SRR26075350.sra Read 819553 spots for SRR26075350.sra Written 819553 spots for SRR26075350.sra Read 819553 spots for SRR26075350.sra Written 819553 spots for SRR26075350.sra Read 819553 spots for SRR26075350.sra Written 819553 spots for SRR26075350.sra Read 819553 spots for SRR26075350.sra Written 819553 spots for SRR26075350.sra Read 819556 spots for SRR26075350.sra Written 819556 spots for SRR26075350.sra Read 819553 spots for SRR26075350.sra Written 819553 spots for SRR26075350.sra Read 819553 spots for SRR26075350.sra Written 819553 spots for SRR26075350.sra SRR ids: ['SRR26075350.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_smp3m8j3 SRR26075350.sra spots: 16391063 blocks: [[1, 819553], [819554, 1639106], [1639107, 2458659], [2458660, 3278212], [3278213, 4097765], [4097766, 4917318], [4917319, 5736871], [5736872, 6556424], [6556425, 7375977], [7375978, 8195530], [8195531, 9015083], [9015084, 9834636], [9834637, 10654189], [10654190, 11473742], [11473743, 12293295], [12293296, 13112848], [13112849, 13932401], [13932402, 14751954], [14751955, 15571507], [15571508, 16391063]] SRR26075350 file size 6047203 SRR26075350 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o SRR26075350 SRR26075350_1.fastq SRR26075350_2.fastq Input file: SRR26075350_1.fastq Paired file: SRR26075350_2.fastq trimmed: SRR26075350-trimmed-pair1.fastq, SRR26075350-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Tue Feb 11 21:22:35 2025 >> started Tue Feb 11 21:22:54 2025 >> done (19.256s) 16391063 read pairs processed; of these: 66 ( 0.00%) short read pairs filtered out after trimming by size control 101571 ( 0.62%) empty read pairs filtered out after trimming by size control 16289426 (99.38%) read pairs available; of these: 2611115 (16.03%) trimmed read pairs available after processing 13678311 (83.97%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 18 1 0.00% 19 2 0.00% 20 5 0.00% 21 6 0.00% 22 11 0.00% 23 6 0.00% 24 11 0.00% 25 12 0.00% 26 11 0.00% 27 17 0.00% 28 12 0.00% 29 8 0.00% 30 14 0.00% 31 28 0.00% 32 19 0.00% 33 19 0.00% 34 27 0.00% 35 24 0.00% 36 17 0.00% 37 21 0.00% 38 21 0.00% 39 23 0.00% 40 43 0.00% 41 26 0.00% 42 26 0.00% 43 36 0.00% 44 35 0.00% 45 50 0.00% 46 37 0.00% 47 49 0.00% 48 73 0.00% 49 76 0.00% 50 73 0.00% 51 75 0.00% 52 111 0.00% 53 121 0.00% 54 133 0.00% 55 139 0.00% 56 148 0.00% 57 172 0.00% 58 223 0.00% 59 222 0.00% 60 284 0.00% 61 332 0.00% 62 342 0.00% 63 363 0.00% 64 529 0.00% 65 544 0.00% 66 675 0.00% 67 590 0.00% 68 786 0.00% 69 842 0.01% 70 922 0.01% 71 1117 0.01% 72 1293 0.01% 73 1580 0.01% 74 1686 0.01% 75 2037 0.01% 76 2197 0.01% 77 2507 0.02% 78 2861 0.02% 79 3100 0.02% 80 3408 0.02% 81 3910 0.02% 82 4500 0.03% 83 5052 0.03% 84 5950 0.04% 85 6438 0.04% 86 6927 0.04% 87 7496 0.05% 88 8217 0.05% 89 8870 0.05% 90 9426 0.06% 91 10272 0.06% 92 11331 0.07% 93 12568 0.08% 94 13893 0.09% 95 14447 0.09% 96 15428 0.09% 97 16916 0.10% 98 18019 0.11% 99 18456 0.11% 100 19049 0.12% 101 20298 0.12% 102 20734 0.13% 103 22450 0.14% 104 23817 0.15% 105 25574 0.16% 106 26252 0.16% 107 27557 0.17% 108 28322 0.17% 109 29712 0.18% 110 30134 0.18% 111 31271 0.19% 112 31771 0.20% 113 33435 0.21% 114 34233 0.21% 115 36048 0.22% 116 37963 0.23% 117 38302 0.24% 118 40324 0.25% 119 40963 0.25% 120 41595 0.26% 121 43171 0.27% 122 42717 0.26% 123 45000 0.28% 124 45651 0.28% 125 46823 0.29% 126 48173 0.30% 127 49991 0.31% 128 51155 0.31% 129 52407 0.32% 130 53676 0.33% 131 53689 0.33% 132 53967 0.33% 133 54720 0.34% 134 55989 0.34% 135 57630 0.35% 136 58822 0.36% 137 60510 0.37% 138 61015 0.37% 139 62474 0.38% 140 63204 0.39% 141 64637 0.40% 142 64131 0.39% 143 65515 0.40% 144 66630 0.41% 145 67778 0.42% 146 69162 0.42% 147 69283 0.43% 148 70566 0.43% 149 71093 0.44% 150 73438 0.45% 151 13678311 83.97% 16289426 reads passed initial QC criterion=sequence-density sequence-density=0.26 sequence-density-rank=1 fanout-score=2.57 fanout-score-rank=32 prefix-density=0.26 prefix-fanout=2.6 sequence=GTCAGGGTACAT criterion=fanout-score sequence-density=0.07 sequence-density-rank=32 fanout-score=63.56 fanout-score-rank=1 prefix-density=0.30 prefix-fanout=15.1 sequence=CATCTCCATCGCAGTTCCATCCTTCCTTTCCCTTTTCAGTTCCAGTATCTTCATCTTCCTTCAAAGCACACTTAGGGTGAAGATCAAAGTCACATTGTTTGCAAT criterion=sequence-density sequence-density=0.46 sequence-density-rank=1 fanout-score=2.66 fanout-score-rank=27 prefix-density=0.47 prefix-fanout=2.6 sequence=ATGTACCCTGAC criterion=fanout-score sequence-density=0.01 sequence-density-rank=31 fanout-score=100.63 fanout-score-rank=1 prefix-density=0.09 prefix-fanout=8.1 sequence=AGAAAAGATAAGCTAGGCAAGATGGTTTTACTAGACAAGATGTGGGATGATGTTGTTGCTGGACCTCAGCCAGAACGTGGCCTTGGCAAGCTTAGAAAGATCAGCACCAGACCACTTAACATCAAAGATATTGACGTCGGAGAGGGGAGCAGTCCTGTTAATAAGTTTCAGAGGTCCATGACTATGCCAGGAACTCCAGGGACACCGACGACACCAGTGACCCCTACAACCCCAGTGTCGGCGCGTAGCAATGTTTGGAGGAGCGTGTTCCACCCTGGTAGCAACCTTGCTACTAAGAATATTGGTGCTCATGTTTTTGACAAGCCACAGCCTAACACACCCACTGTCTATGACTGGATGTACAGTGGAGAGACGAAGAGCGAGCATCGTTGATGAGGTTGCCTTCAACCAAGGTTGCCCATGTAAATACGTACTGTGTTTTGTTTTTCAGTACT SRR26075350 testing PE reads STAR mapping to Ensembl genome Started job on | Feb 11 21:23:38 Started mapping on | Feb 11 21:23:38 Finished on | Feb 11 21:26:44 Mapping speed, Million of reads per hour | 315.28 Number of input reads | 16289426 Average input read length | 294 UNIQUE READS: Uniquely mapped reads number | 14399933 Uniquely mapped reads % | 88.40% Average mapped length | 292.67 Number of splices: Total | 13603269 Number of splices: Annotated (sjdb) | 13272694 Number of splices: GT/AG | 13358744 Number of splices: GC/AG | 185660 Number of splices: AT/AC | 13050 Number of splices: Non-canonical | 45815 Mismatch rate per base, % | 0.39% Deletion rate per base | 0.03% Deletion average length | 3.28 Insertion rate per base | 0.02% Insertion average length | 2.61 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 441452 % of reads mapped to multiple loci | 2.71% Number of reads mapped to too many loci | 55925 % of reads mapped to too many loci | 0.34% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 8.16% % of reads unmapped: other | 0.38% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 1448041 1448041 1448041 N_multimapping 441452 441452 441452 N_noFeature 362268 14210535 467920 N_ambiguous 164040 964 79645 UnstrandedReadsAssigned:13873625 PositiveStrandReadsAssigned:188434 NegativeStrandReadsAssigned:13852368 Dataset is classified negative stranded MeadianReadLen=151 20thPercentileLength=151 echo kmer=147 SRR26075350 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,400 [index] number of k-mers: 62,057,036 [index] number of equivalence classes: 130,681 [quant] running in paired-end mode [quant] will process pair 1: SRR26075350-trimmed-pair1.fastq SRR26075350-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 16,289,426 reads, 13,998,973 reads pseudoaligned [quant] estimated average fragment length: 214.729 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,122 rounds 52401 SRR26075350.ke.tsv 34699 SRR26075350.se.tsv 87100 total ==> SRR26075350.ke.tsv <== target_id length eff_length est_counts tpm Potri.005G200100.1.v4.1 2018 1804.27 2007 67.1463 Potri.005G024800.1.v4.1 1035 821.271 740 54.3903 Potri.004G059700.1.v4.1 961 747.277 11 0.888562 Potri.007G009000.2.v4.1 1416 1202.27 0 0 Potri.003G141000.2.v4.1 2943 2729.27 593 13.1155 Potri.016G087400.1.v4.1 270 88.8065 1324 899.953 Potri.015G069301.1.v4.1 564 351.92 0 0 Potri.010G195200.1.v4.1 1773 1559.27 227 8.78782 Potri.012G127500.1.v4.1 977 763.277 10905 862.422 ==> SRR26075350.se.tsv <== Potri.001G166300.v4.1 0 Potri.001G448400.v4.1 96 Potri.001G233950.v4.1 0 Potri.001G122700.v4.1 335 Potri.001G212900.v4.1 0 Potri.001G182400.v4.1 0 Potri.001G256600.v4.1 0 Potri.001G040500.v4.1 0 Potri.001G416900.v4.1 0 Potri.001G452600.v4.1 725 SRR26075350 completed mapping pipeline successfully