Starting /dee2/code/volunteer_pipeline.sh SRR26075351 current disk space = 3052618092544 free memory = 1490396672 SRR26075351 SRAfilesize 69672871a116322a6edd2263bee224f8 SRR26075351.sra SRR26075351.sra file validated SRR26075351 is paired end SRR26075351 is conventional basespace SRR26075351 read1 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR26075351_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.658 37.0 37.0 37.0 37.0 37.0 2 36.6175 37.0 37.0 37.0 37.0 37.0 3 36.555 37.0 37.0 37.0 37.0 37.0 4 36.7015 37.0 37.0 37.0 37.0 37.0 5 36.6395 37.0 37.0 37.0 37.0 37.0 6 36.6625 37.0 37.0 37.0 37.0 37.0 7 36.5995 37.0 37.0 37.0 37.0 37.0 8 36.6875 37.0 37.0 37.0 37.0 37.0 9 36.6695 37.0 37.0 37.0 37.0 37.0 10-14 36.62525 37.0 37.0 37.0 37.0 37.0 15-19 36.586200000000005 37.0 37.0 37.0 37.0 37.0 20-24 36.5192 37.0 37.0 37.0 37.0 37.0 25-29 36.4523 37.0 37.0 37.0 37.0 37.0 30-34 36.432500000000005 37.0 37.0 37.0 37.0 37.0 35-39 36.3745 37.0 37.0 37.0 37.0 37.0 40-44 36.327600000000004 37.0 37.0 37.0 37.0 37.0 45-49 36.2467 37.0 37.0 37.0 37.0 37.0 50-54 36.175700000000006 37.0 37.0 37.0 37.0 37.0 55-59 36.1045 37.0 37.0 37.0 37.0 37.0 60-64 36.0358 37.0 37.0 37.0 37.0 37.0 65-69 35.9687 37.0 37.0 37.0 37.0 37.0 70-74 36.0101 37.0 37.0 37.0 37.0 37.0 75-79 35.9819 37.0 37.0 37.0 37.0 37.0 80-84 35.9868 37.0 37.0 37.0 37.0 37.0 85-89 35.876 37.0 37.0 37.0 37.0 37.0 90-94 35.902100000000004 37.0 37.0 37.0 37.0 37.0 95-99 35.949 37.0 37.0 37.0 37.0 37.0 100-104 35.8493 37.0 37.0 37.0 37.0 37.0 105-109 35.7903 37.0 37.0 37.0 37.0 37.0 110-114 35.6716 37.0 37.0 37.0 37.0 37.0 115-119 35.6118 37.0 37.0 37.0 37.0 37.0 120-124 35.58729999999999 37.0 37.0 37.0 37.0 37.0 125-129 35.502 37.0 37.0 37.0 37.0 37.0 130-134 35.3606 37.0 37.0 37.0 29.8 37.0 135-139 35.2295 37.0 37.0 37.0 34.6 37.0 140-144 35.1341 37.0 37.0 37.0 29.8 37.0 145-149 35.064299999999996 37.0 37.0 37.0 27.4 37.0 150-151 34.89025 37.0 37.0 37.0 25.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.0 1101 2 0.0 1101 3 0.0 1101 4 0.0 1101 5 0.0 1101 6 0.0 1101 7 0.0 1101 8 0.0 1101 9 0.0 1101 10-14 0.0 1101 15-19 0.0 1101 20-24 0.0 1101 25-29 0.0 1101 30-34 0.0 1101 35-39 0.0 1101 40-44 0.0 1101 45-49 0.0 1101 50-54 0.0 1101 55-59 0.0 1101 60-64 0.0 1101 65-69 0.0 1101 70-74 0.0 1101 75-79 0.0 1101 80-84 0.0 1101 85-89 0.0 1101 90-94 0.0 1101 95-99 0.0 1101 100-104 0.0 1101 105-109 0.0 1101 110-114 0.0 1101 115-119 0.0 1101 120-124 0.0 1101 125-129 0.0 1101 130-134 0.0 1101 135-139 0.0 1101 140-144 0.0 1101 145-149 0.0 1101 150-151 0.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 21 3.0 22 2.0 23 7.0 24 2.0 25 8.0 26 9.0 27 10.0 28 21.0 29 34.0 30 31.0 31 42.0 32 52.0 33 115.0 34 161.0 35 421.0 36 2886.0 37 196.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.74374374374375 12.912912912912914 8.608608608608609 34.73473473473474 2 18.875 12.950000000000001 33.1 35.075 3 18.325 18.325 29.15 34.2 4 21.325 27.450000000000003 24.75 26.474999999999998 5 23.425 30.9 25.374999999999996 20.3 6 23.225 34.425 21.3 21.05 7 14.274999999999999 31.15 37.45 17.125 8 18.4 28.299999999999997 30.65 22.650000000000002 9 19.325 22.575 34.775 23.325000000000003 10-14 20.066003300165008 29.091454572728637 27.706385319265962 23.13615680784039 15-19 20.555 27.189999999999998 27.700000000000003 24.555 20-24 20.724999999999998 27.21 27.615000000000002 24.45 25-29 19.919999999999998 28.08 26.965 25.035 30-34 19.8 27.825 27.800000000000004 24.575 35-39 20.44 26.695 28.43 24.435000000000002 40-44 20.31 28.88 27.169999999999998 23.64 45-49 20.805 27.589999999999996 27.605 24.0 50-54 20.4 26.790000000000003 28.775000000000002 24.035 55-59 20.845 28.17 27.639999999999997 23.345 60-64 20.64 27.725 27.515 24.12 65-69 21.029999999999998 28.105000000000004 27.16 23.705000000000002 70-74 20.96 27.275 26.875 24.89 75-79 21.044999999999998 27.045 27.700000000000003 24.21 80-84 20.925 27.27 27.725 24.08 85-89 21.215 26.884999999999998 28.060000000000002 23.84 90-94 21.42 26.77 27.96 23.849999999999998 95-99 21.055 28.205000000000002 26.445 24.295 100-104 21.335 28.315 26.939999999999998 23.41 105-109 21.315 28.084999999999997 26.640000000000004 23.96 110-114 21.32 26.935 27.805000000000003 23.94 115-119 21.495 28.285 26.674999999999997 23.544999999999998 120-124 20.880000000000003 28.955 25.845000000000002 24.32 125-129 21.86 27.345000000000002 27.155 23.64 130-134 21.255 28.255000000000003 26.834999999999997 23.655 135-139 22.065 28.105000000000004 25.835 23.995 140-144 21.23 28.345 26.314999999999998 24.11 145-149 22.145 27.150000000000002 26.150000000000002 24.555 150-151 22.5 28.125 25.1875 24.1875 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 1.0 8 1.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.5 16 0.5 17 0.0 18 0.0 19 0.0 20 0.0 21 0.5 22 1.0 23 2.5 24 2.0 25 1.5 26 3.0 27 6.5 28 8.0 29 12.5 30 18.5 31 18.0 32 27.0 33 39.5 34 49.0 35 62.5 36 63.5 37 77.5 38 100.0 39 133.5 40 169.0 41 193.0 42 231.5 43 252.5 44 254.0 45 273.5 46 279.0 47 251.5 48 234.0 49 225.0 50 200.0 51 142.0 52 112.0 53 106.5 54 94.0 55 89.5 56 60.0 57 41.5 58 36.5 59 19.0 60 15.0 61 13.5 62 13.5 63 12.5 64 11.0 65 8.5 66 4.0 67 8.5 68 8.0 69 4.5 70 3.5 71 2.0 72 1.5 73 0.5 74 0.0 75 0.0 76 0.0 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.1 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.005 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150-151 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 61.275 #Duplication Level Percentage of deduplicated Percentage of total 1 64.17788657690738 39.324999999999996 2 21.78702570379437 26.700000000000003 3 7.507139942880457 13.8 4 3.2231742146062827 7.9 5 1.7135862913096693 5.25 6 0.5711954304365565 2.1 7 0.44879640962872297 1.925 8 0.44879640962872297 2.1999999999999997 9 0.04079967360261118 0.22499999999999998 >10 0.08159934720522236 0.575 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CCGGTCATTCCCATGCAGGATCAGCCCCCAGTTGGCACTTGCTGCAGTCC 13 0.325 No Hit GCCCAGTTCCCTCGTTACCAAGTAAGAAAGCAGTACTTCTCTTAAAAGGG 10 0.25 No Hit AGGGCATCCTTTATTATCATAGCACTCAAAAGACTCACGATCGAGGAGAT 9 0.22499999999999998 No Hit GCCAGGTGGACCATGAACACAGCAAACCCAATTGGAAGTGGAGCCAAAAC 8 0.2 No Hit CCTGTCATTAGCTTCCCACCAAGAACTATTTGGATTGGAAGAAGCCTAGC 8 0.2 No Hit GGGAAGAGCAGATGTAGGGGTTATGACTGGAACTCCTCCCAGAGGTTTTG 8 0.2 No Hit GACACAAAATTTAAAGTTCTGGCACTCGCACTTGAACATCGCGATGAGGT 8 0.2 No Hit GATACAGATTATGGCTGAGTAAAAGGAATATATTCCAAACCTTATATCTG 8 0.2 No Hit CTCAATAATATGTTGAAGACAAGACCCCCCAACCATGTATACCAAAACAG 8 0.2 No Hit GCAATCCTACTCCTGCAAACAGCACAACGAAACCACCAAATGCTTGGCAG 8 0.2 No Hit GTCAGCAAGTGTCCTTCCATCTTCAAGCTGTTTTCCAGCAAAAATCAACC 8 0.2 No Hit GCAGAAAATAACCTCGCTCACTCCTCGGCGTCGGCGGGAAGCTCGATCAA 8 0.2 No Hit CCAACTCATAGTCTATATCACCGTCTCCAACAACGTCCTTCACCAAGCTC 8 0.2 No Hit CTCGAAATCAGATAATGGGGTCATCTGCTCCTTCAAACCCTTTCGCTTCC 8 0.2 No Hit CTTCACCATGGCCATCCCCGTCCTTGTACTTACCATGTTCTGGCTTGTGC 7 0.17500000000000002 No Hit GCCACTTAAATCCAGCCCCAAGGTCTTCGGGTCACCGTCATAAATTGCAA 7 0.17500000000000002 No Hit CTGCACGGTGTGAAGTCTTTCTGTTTGAAGGGTTGAGTCATCGGAAGAGG 7 0.17500000000000002 No Hit CTCGTGTGCTTAAACCTCCCACCAGAAGATTGTTCCGCCTATCGGAACCC 7 0.17500000000000002 No Hit CCAACGATGACTCCAACACCGACAACATCTCCAACTTTGAACTTTGTCAC 7 0.17500000000000002 No Hit AGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACGTTCCC 7 0.17500000000000002 No Hit GTCTTGAAGTCCTGGGCAATTTCACGAACAAGCCTCTGGAATGGGAGTTT 7 0.17500000000000002 No Hit CCTGAAAGCAGCACTGTCATAAGCTTTTGCAGCTTCAATGGCTGTATCAA 7 0.17500000000000002 No Hit CTCCTGAATGGGGGCCGCTTGTGTCGCGCTCCTGCTTCGACAAAGTAAAG 7 0.17500000000000002 No Hit CATTGTTCTTAAACCATTTATTCTGGGCTTCATCAACTATAAGAAACTCT 7 0.17500000000000002 No Hit GACGTGAAGTGCACCTGGCCAGTAAAGGCTGAGCCTTGGGCATACTGAGT 7 0.17500000000000002 No Hit ATGGAGTTTTCATACTGCAGAGATGTCCTAAACTGCCTTGCTTCTCCCCA 6 0.15 No Hit ACTTAAATTGTCGTAATTATGGTCCCCCTTGCCATAATTTTCAAAGTTGG 6 0.15 No Hit GATCGGAAGAGCACACGTCTGAACTCCAGTCACAACGGTCAATCTCGTTT 6 0.15 TruSeq Adapter, Index 1 (97% over 36bp) GCTTGGCATACATGAGATCAAACTTGTGGTCAATGCGAGAGAAGACTTCT 6 0.15 No Hit AGAATGCAAAATCATCACTTGGATCACCCCTTAGTTTTGTTTCTGGTTTC 6 0.15 No Hit GTAACATCCCACCCTCGCTCCTTAATGCCCTAACAGCAGTAGCCCAACTG 6 0.15 No Hit TTCGAAACCCATCTTTTTCGACCCAATTCTTTTACCATACCAATAGTTTG 6 0.15 No Hit CCGGCGCCCCTTTCCAGGGGACTTGGGCCTGGTCCGCCGCTGAGGACGCT 6 0.15 No Hit CCCACGTGGAAAATACAAGTCATTGATACAAAAAATCTTGAAAATAGAAG 6 0.15 No Hit GATCGGAAGAGCACACGTCTGAACTCCAGTCACAACGGTCAATCTCGTAT 6 0.15 TruSeq Adapter, Index 1 (97% over 36bp) TGTTGATGGCATGGGATATCCCGGCAACAACACCTACCAAGTTAACGATG 6 0.15 No Hit CCTTAGCTTTGGTCTTAGCCAGAGATTTGGAACCCATGATGCCACCCCCC 6 0.15 No Hit AGATAGGTCTTCACCAATTGGGATTTCATAGAATTGCTTAGTCTCGTACC 6 0.15 No Hit GTCTACTTGAAGAATCCTTGAGCCATCTTGAAAGCTCCCCCGAGCCCACT 6 0.15 No Hit ATGCAGAGTAGGCCTCAGAATCTTCTTTGGCCACCGCCCCATCTTCCATT 5 0.125 No Hit CACTTTTTTATTATATCAAGTAAATTCAGGTCTGATGCATCCTCATGTAT 5 0.125 No Hit AGCGGCCGATGCAACCAGTGCACAACATAAAGTCAGATAAACCTGCTTGA 5 0.125 No Hit TCATACAAGATTTTCCGAGTCTGCAGAGGAAGACCATGCGATATGTAATA 5 0.125 No Hit GTATCCAATCAGTGACACTAGATGGAGGTGGCGAAGATTGGATAACATCT 5 0.125 No Hit TCTGCCTCTTCTTGCTTGAATGCCTTCGAGATCTCCTCAATCGGAACCAC 5 0.125 No Hit GGAGGGAAATGGATGGTTACTAGAAACACCCCACCTGCATATGGACTATC 5 0.125 No Hit ATTCGTCGCCAGGCAGCTATACGGCTGTAAAGAGCTGGGAAAAGGAAGCA 5 0.125 No Hit GTTATAATCAGGTGGCGGCGGCGGAGTAGGAGGGGGATAATAATCACCAC 5 0.125 No Hit TGGAAGTGAAAATAAAATTACTGGTGAGCTAAGCCAGGTAAAACTAGCTA 5 0.125 No Hit GGTGGAAGTCTAGAGACATGATAGGTGAACTATGCCCTGTATACTCATGC 5 0.125 No Hit ATGGAGGAGCTGGGTCTTGGTTGGGTGTAGCCGTGCGTGGAGAAGGAGCT 5 0.125 No Hit AGGGGCTATGACAGAGTAGCAAAGAACAATTGTGAGAACAAGCATGTCAC 5 0.125 No Hit AGGTTATTCAGAGCCCACAATATCCAAGTGTTCCCTTTTTTGGAAATTTT 5 0.125 No Hit GGAACCAACAAAGCAGCAGGAAATACAAGACACTTACAGATTACTAGCCA 5 0.125 No Hit GTTCAACATAGTTTTATCAGATGGCTGCGCTGCTGCTGCTGCTGCTGGCG 5 0.125 No Hit GTTATAACACTAAACCTAAAAATTATCAAAGGAAATCTCTTTTCAAGACC 5 0.125 No Hit CCGCGGGATTGAGCTTCGTTCACGGTGATGTTACGGCCATCGAGGTCCTG 5 0.125 No Hit GTCCTTTTCATCTTTCCCTCGCGGTACTTGTTTGCTATCGGTCTCTCGCC 5 0.125 No Hit CTCCTGGGGAACAAGGCATCCACCCTTCCCTCTTGATTGGCCATGAAGAT 5 0.125 No Hit CCTTCAACTAGCTTAGCATAAGGATCAATAAGCATGATGCTGCTATCAAA 5 0.125 No Hit CATTGTATCTGCTTGCCCTGCATGATTTCCATTGTTCTCTTCCGCATGCT 5 0.125 No Hit GGCAGAACATGCAATGCTGTTAAATCTGGTCCCATTCTCTGACAAGCTGC 5 0.125 No Hit GTCAACCTCGCCTAAAATAACGGCCATCTCCTCGCTGCTCGGCGGCTCGG 5 0.125 No Hit GCCTTGTATAGCATCAAAAACAGGACGGTGTGAGTAAGGGAATTGTGTGT 5 0.125 No Hit GTTGAAAGGAAAGGTTTTTCTGTGTTGTTTGAGATTTGTGAGGGGGGGGT 5 0.125 No Hit CGAGTAACGAGAGAGCTAAGCCATGTAGGACATCAAACGCAATCACATAT 5 0.125 No Hit GCTCAGGTCCATTAAGCCTGTTGTTTGCGAAACTTTCCATCGGAAACGAG 5 0.125 No Hit AGCAGTTTCAGTAACGGCAACGGCAACAGCCGCAGCCGTCAACGAAGCAC 5 0.125 No Hit CTGTACACTGCTTGGGGCAGTGGAATTAGAGGGCTGGTTGTCCTTTTATA 5 0.125 No Hit CAGGCACTGATGTGGAACCAAAGGATCCAAATATACCTTCTGTAATCCTT 5 0.125 No Hit GTAACAGCAAAAACCGCAGATGCATAGGTTATCATCATTGAAGCTGTAGC 5 0.125 No Hit GCCCTCCATTTGCTTCCCTTTCACCCATTCAGTAGCAACAGAGGAGGAAG 5 0.125 No Hit ATATAATCAAAGCTACGTGTAACCAACTGATTGGCTACAGAGCATATTTA 5 0.125 No Hit TAAAAATGTTGGCACAGGATGTGGAAGAGCCAACAAAGTGATGTGGACCT 5 0.125 No Hit CACATAACACCATTCTCTTTGGTGAATTCCGCAGCAGGAACCATCACCAA 5 0.125 No Hit GCACAACTTTGTATACTTCAATCAGGAGCTTCGGGAAGCGACTCGCAAAA 5 0.125 No Hit TCTAAACATTGAATTAATTTCAAGGACATGATTTGGGGTAAATGTCCAAC 5 0.125 No Hit CAGCGGATTGAACTCGTTAGCATCTTTTCCCCATATATCAGTGTCATGAT 5 0.125 No Hit GAATCGATGATTTCACCGTACTGGCTAAAAGCCTCTTGGAGGACTTGGTC 5 0.125 No Hit GGGTGATATCGATCACTTGGTAATGATATAGACGGCATAAATGATTCCAG 5 0.125 No Hit CGGGACGGTATTAAACTAAAGTCTAAAGCCATCAGGCGATCTACATGAAA 5 0.125 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0 0.0 0.0 0.0 0.0 64-65 0.0 0.0 0.0 0.0 0.0 66-67 0.0 0.0 0.0 0.0 0.0 68-69 0.0 0.0 0.0 0.0 0.0 70-71 0.0 0.0 0.0 0.0 0.0 72-73 0.0 0.0 0.0 0.0 0.0 74-75 0.0125 0.0 0.0 0.0 0.0 76-77 0.05 0.0 0.0 0.0 0.0 78-79 0.0875 0.0 0.0 0.0 0.0 80-81 0.125 0.0 0.0 0.0 0.0 82-83 0.175 0.0 0.0 0.0 0.0 84-85 0.25 0.0 0.0 0.0 0.0 86-87 0.30000000000000004 0.0 0.0 0.0 0.0 88-89 0.325 0.0 0.0 0.0 0.0 90-91 0.4 0.0 0.0 0.0 0.0 92-93 0.5625 0.0 0.0 0.0 0.0 94-95 0.75 0.0 0.0 0.0 0.0 96-97 1.0 0.0 0.0 0.0 0.0 98-99 1.1 0.0 0.0 0.0 0.0 100-101 1.3125 0.0 0.0 0.0 0.0 102-103 1.575 0.0 0.0 0.0 0.0 104-105 1.85 0.0 0.0 0.0 0.0 106-107 2.0875000000000004 0.0 0.0 0.0 0.0 108-109 2.425 0.0 0.0 0.0 0.0 110-111 2.5625 0.0 0.0 0.0 0.0 112-113 2.925 0.0 0.0 0.0 0.0 114-115 3.2375 0.0 0.0 0.0 0.0 116-117 3.7125000000000004 0.0125 0.0 0.0 0.0 118-119 4.1 0.025 0.0 0.0 0.0 120-121 4.362500000000001 0.025 0.0 0.0 0.0 122-123 4.9625 0.025 0.0 0.0 0.0 124-125 5.5125 0.025 0.0 0.0 0.0 126-127 6.2125 0.025 0.0 0.0 0.0 128-129 7.0125 0.025 0.0 0.0 0.0 130-131 7.5125 0.025 0.0 0.0 0.0 132-133 7.925 0.025 0.0 0.0 0.0 134-135 8.2375 0.025 0.0 0.0 0.0 136-137 8.7875 0.025 0.0 0.0 0.0 138-139 9.662500000000001 0.025 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TGCCTCT 10 0.006830828 145.0 3 TCTGCCT 10 0.006830828 145.0 1 TTCTTGC 10 0.006830828 145.0 9 CTGCCTC 10 0.006830828 145.0 2 >>END_MODULE SRR26075351 read2 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR26075351_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.15375 37.0 37.0 37.0 37.0 37.0 2 36.0865 37.0 37.0 37.0 37.0 37.0 3 36.141 37.0 37.0 37.0 37.0 37.0 4 36.079 37.0 37.0 37.0 37.0 37.0 5 36.2335 37.0 37.0 37.0 37.0 37.0 6 36.071 37.0 37.0 37.0 37.0 37.0 7 36.094 37.0 37.0 37.0 37.0 37.0 8 36.1335 37.0 37.0 37.0 37.0 37.0 9 36.17 37.0 37.0 37.0 37.0 37.0 10-14 36.0878 37.0 37.0 37.0 37.0 37.0 15-19 36.0179 37.0 37.0 37.0 37.0 37.0 20-24 35.849599999999995 37.0 37.0 37.0 37.0 37.0 25-29 35.7327 37.0 37.0 37.0 37.0 37.0 30-34 35.5911 37.0 37.0 37.0 37.0 37.0 35-39 35.6058 37.0 37.0 37.0 37.0 37.0 40-44 35.4978 37.0 37.0 37.0 37.0 37.0 45-49 35.4529 37.0 37.0 37.0 37.0 37.0 50-54 35.3339 37.0 37.0 37.0 37.0 37.0 55-59 35.3328 37.0 37.0 37.0 37.0 37.0 60-64 35.4461 37.0 37.0 37.0 37.0 37.0 65-69 35.3815 37.0 37.0 37.0 37.0 37.0 70-74 35.271100000000004 37.0 37.0 37.0 37.0 37.0 75-79 35.202299999999994 37.0 37.0 37.0 37.0 37.0 80-84 35.24210000000001 37.0 37.0 37.0 37.0 37.0 85-89 35.1682 37.0 37.0 37.0 34.6 37.0 90-94 35.1848 37.0 37.0 37.0 34.6 37.0 95-99 35.160999999999994 37.0 37.0 37.0 34.6 37.0 100-104 35.0785 37.0 37.0 37.0 29.8 37.0 105-109 35.0934 37.0 37.0 37.0 29.8 37.0 110-114 35.0209 37.0 37.0 37.0 27.4 37.0 115-119 34.9427 37.0 37.0 37.0 27.4 37.0 120-124 34.8386 37.0 37.0 37.0 25.0 37.0 125-129 34.86639999999999 37.0 37.0 37.0 25.0 37.0 130-134 34.839 37.0 37.0 37.0 25.0 37.0 135-139 34.67504999999999 37.0 37.0 37.0 25.0 37.0 140-144 34.70125 37.0 37.0 37.0 25.0 37.0 145-149 34.684749999999994 37.0 37.0 37.0 25.0 37.0 150-151 34.481125 37.0 37.0 37.0 25.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.0 1101 2 0.0 1101 3 0.0 1101 4 0.0 1101 5 0.0 1101 6 0.0 1101 7 0.0 1101 8 0.0 1101 9 0.0 1101 10-14 0.0 1101 15-19 0.0 1101 20-24 0.0 1101 25-29 0.0 1101 30-34 0.0 1101 35-39 0.0 1101 40-44 0.0 1101 45-49 0.0 1101 50-54 0.0 1101 55-59 0.0 1101 60-64 0.0 1101 65-69 0.0 1101 70-74 0.0 1101 75-79 0.0 1101 80-84 0.0 1101 85-89 0.0 1101 90-94 0.0 1101 95-99 0.0 1101 100-104 0.0 1101 105-109 0.0 1101 110-114 0.0 1101 115-119 0.0 1101 120-124 0.0 1101 125-129 0.0 1101 130-134 0.0 1101 135-139 0.0 1101 140-144 0.0 1101 145-149 0.0 1101 150-151 0.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 12 1.0 13 8.0 14 20.0 15 14.0 16 11.0 17 7.0 18 12.0 19 8.0 20 6.0 21 11.0 22 15.0 23 18.0 24 16.0 25 12.0 26 19.0 27 19.0 28 14.0 29 19.0 30 34.0 31 23.0 32 43.0 33 71.0 34 214.0 35 663.0 36 2541.0 37 181.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 48.21205301325332 22.80570142535634 9.202300575143786 19.779944986246562 2 32.05 23.925 24.8 19.225 3 23.325000000000003 29.099999999999998 28.875 18.7 4 26.875 34.775 20.200000000000003 18.15 5 27.500000000000004 35.775 18.35 18.375 6 23.625 36.75 22.0 17.625 7 24.65 23.025000000000002 33.2 19.125 8 24.25 25.374999999999996 25.724999999999998 24.65 9 24.625 27.500000000000004 26.974999999999998 20.9 10-14 25.71 28.975 24.995 20.32 15-19 25.805 28.455000000000002 26.179999999999996 19.56 20-24 25.685000000000002 27.88 26.31 20.125 25-29 25.685000000000002 27.85 26.200000000000003 20.265 30-34 25.080000000000002 27.189999999999998 27.51 20.22 35-39 24.555 28.005000000000003 26.375 21.065 40-44 25.564999999999998 28.720000000000002 26.215 19.5 45-49 25.64 27.675 26.035000000000004 20.65 50-54 22.895 28.99 27.139999999999997 20.974999999999998 55-59 25.19 27.185 27.175 20.45 60-64 25.09 27.32 27.21 20.380000000000003 65-69 25.97 28.1 25.22 20.71 70-74 23.705000000000002 28.985 26.625 20.685000000000002 75-79 23.044999999999998 29.054999999999996 27.495000000000005 20.405 80-84 24.515 27.63 26.445 21.41 85-89 25.36 28.555000000000003 26.400000000000002 19.685 90-94 25.22 29.134999999999998 26.02 19.625 95-99 24.795 28.470000000000002 26.884999999999998 19.85 100-104 25.195 28.58 26.41 19.814999999999998 105-109 25.44 28.035 26.375 20.150000000000002 110-114 25.455 28.53 25.045 20.97 115-119 25.255 29.060000000000002 26.169999999999998 19.515 120-124 24.709999999999997 29.65 25.715 19.925 125-129 26.265 29.330000000000002 25.16 19.245 130-134 25.490000000000002 29.185 26.41 18.915000000000003 135-139 25.316265813290666 29.19645982299115 25.98629931496575 19.500975048752437 140-144 25.878881832274843 29.59443916587488 24.913737060559086 19.612941941291194 145-149 27.186796699174792 29.167291822955736 24.98624656164041 18.659664916229058 150-151 27.428428553569194 28.56607075884486 25.040630078759847 18.964870608826104 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.5 3 1.0 4 0.5 5 0.0 6 1.0 7 2.5 8 2.0 9 1.0 10 2.5 11 2.5 12 1.5 13 1.5 14 2.5 15 3.5 16 2.0 17 1.0 18 0.5 19 0.5 20 1.0 21 1.0 22 2.0 23 2.0 24 2.0 25 3.0 26 4.0 27 4.5 28 5.5 29 4.5 30 3.5 31 12.0 32 19.0 33 23.0 34 32.5 35 43.0 36 57.0 37 79.0 38 133.0 39 186.0 40 204.0 41 224.5 42 246.5 43 230.0 44 243.5 45 268.0 46 255.0 47 259.0 48 244.0 49 201.5 50 157.5 51 139.5 52 122.5 53 118.5 54 101.0 55 50.0 56 36.5 57 36.5 58 29.0 59 18.0 60 13.5 61 14.0 62 11.0 63 13.5 64 13.0 65 11.0 66 9.0 67 5.0 68 2.0 69 1.0 70 2.5 71 3.0 72 2.0 73 2.0 74 1.5 75 1.0 76 1.5 77 2.0 78 2.0 79 1.0 80 1.0 81 1.0 82 2.0 83 3.0 84 2.5 85 3.0 86 3.5 87 4.0 88 2.5 89 0.5 90 2.0 91 2.0 92 0.5 93 2.0 94 3.0 95 1.5 96 1.5 97 1.5 98 2.5 99 3.5 100 9.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.025 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.005 140-144 0.015 145-149 0.025 150-151 0.0125 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 62.55 #Duplication Level Percentage of deduplicated Percentage of total 1 66.94644284572342 41.875 2 20.26378896882494 25.35 3 6.594724220623502 12.375 4 3.037569944044764 7.6 5 1.5987210231814548 5.0 6 0.5995203836930456 2.25 7 0.4796163069544364 2.1 8 0.2797761790567546 1.4000000000000001 9 0.07993605115907274 0.44999999999999996 >10 0.1199040767386091 1.6 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG 33 0.8250000000000001 No Hit GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGT 20 0.5 No Hit AACATCTTCTTGGGGTACACATCTTGGCGATGCAGCAACTACACCTGCTC 11 0.27499999999999997 No Hit TGTAAGACGTTCTTGGCAGGAGCATACTTCACTTGCTCTCAGTGTTTCTT 9 0.22499999999999998 No Hit GTTCCAAGGTGTTTCAAATGTAGCTGGTAAAACAAGTTCAGACTCAGGAG 9 0.22499999999999998 No Hit GCTTGCTGCCCTTGGTATGTCAGACTTTCCTGGGATTGTTGAAGTTGAAC 8 0.2 No Hit GTCAAAGTTTTGTTACACTGGAAGACCACCTAGGTGCTGCACTACAGAAG 8 0.2 No Hit GGAGTCCACCCTCCACTTGGTGCTTCGTCTACGTGGTGGCATGCAGATTT 8 0.2 No Hit GGTAGGCGTCTACATATAAGCCTCTTTGTAACTTATCCATTCCTTATTGA 8 0.2 No Hit TGATAAACAAGCTGGAAACCATTATCCTGTGACAACCTATGGTACACAGC 8 0.2 No Hit GTTTGAAGAAATTCAGAGGCCCGGTACCCCCCTGTACAATATCAAGGCGT 8 0.2 No Hit ATCGCGTCTCTTTTCTCGTCTTGGGCATGGCTTCTCCTCGCCGGCTTCAG 8 0.2 No Hit AGAAACTCCATCTCTCTCTCTTAGCCTCATTGTTTCAAGAAAATGGGTAG 7 0.17500000000000002 No Hit AGGGGAGATCGCACGGGGATTTCCCGGGTTCTCCCCTCTAGTCTCTGTGT 7 0.17500000000000002 No Hit CCCAAGGAAGCAGCTCGCTACTAAGGCTGCCCGTAAGTCTGCCCCAACCA 7 0.17500000000000002 No Hit CAAAAATTATAAGAACAGAGCCCTCAGATCTCCTTTTATGGAGTCTGGTT 7 0.17500000000000002 No Hit GCGCAGTCTGTGGTGTTGGCTTGGTCAAGGCCTTCATGAAGCCATACTAC 7 0.17500000000000002 No Hit AGTACAGCCAGTGGTGATCAAGGCAGATGTTTCAAGTGAACAAGAAAGGC 7 0.17500000000000002 No Hit CTGAATGATTCTGAGAATGTTTTGGTTGCTGAAACTAAAGATGCATCAGA 7 0.17500000000000002 No Hit GGCGGGACTACCCGCTGAGTTTAAGCATATCAATAAGCGGAGGAAAAGAA 7 0.17500000000000002 No Hit CCAGGGCAGAATAAGAAGGGTGATTGCTAACTACTGCTATTCTTTTATAG 7 0.17500000000000002 No Hit AGAGAGAAAAGGAAAGACACCATGGCAGGAATCATGCACAAGATTGAGGA 7 0.17500000000000002 No Hit GGAACAAAAGGGTAAAAGCTCGTTTGATTCTGATTTCCAGTACGAATACG 7 0.17500000000000002 No Hit TGACTAATTAAGTTTATCTTGCAATTACAAAAATGTCGCAGTTGCTAGGC 7 0.17500000000000002 No Hit GTTCTGAAATATGATGCACATTTTATCTTTGGGGCTTCTGAGTTGGCCGA 6 0.15 No Hit GGAGTTTCACTTTCTGGAAACAGGCAAAGTGGCCGGGCTTTTTTTGGCAT 6 0.15 No Hit GGAGTCTGACATGTGTGCGAGTCAACGGGCGAGTAAACCCGTAAGGCGCA 6 0.15 No Hit GTGCAAAGGTTCAAAGGGTGATTCTTTCTCTTCAAATCCGATCATGGAAG 6 0.15 No Hit CCAACTGTTGTTCCAGGAGGCGACCTTGCTAAGGTTCAGAGGGCTGTTTG 6 0.15 No Hit GTTACTGCCTCCGAAATGCTTCCGCACGTGTTGGACAATGTTGTCAGGAA 6 0.15 No Hit GTTTTACTAGACAAGATGTGGGATGATGTTGTTGCTGGACCTCAGCCAGA 6 0.15 No Hit ACAAATATGAGGCAGCAACAATCTGTTCAAGGAGAAAGCAACTGTATATT 6 0.15 No Hit CTTACATCAGTGAAGAATGAGGATAAATTGGAGTTTAGCAAGGTTTTGGA 6 0.15 No Hit GCTCAAAGTACTCGCTGGAATTGACACCAACTTCACTGTCACGTCCAAGT 6 0.15 No Hit GGGAAGGTTTGGCCATGCGCATGGCTGGATTGACTCTGGGGTCATTACTT 6 0.15 No Hit AGACTCTCATCAGAAAAGCTTAGACTCATCCGATTTAGGGTTTCGCTTCA 6 0.15 No Hit GTTGTCTCTGGCAGGGTTCTGTAAGGGTTTTGCTATCTCCCTCTCTCCCA 6 0.15 No Hit AGCAAAACTAGTTTATGCTTGTGAATGGAATCCTCATGCTGTTGAGGCAC 6 0.15 No Hit AGAAAGTTGAACAAAAAGCAGAAAAAATACAAGTACCATCCAGTTGCAGG 6 0.15 No Hit CAGAGTCAGACTCAGTGAAGAAATCAGAGGAGCTGAAGAGGAAGGCTAGA 5 0.125 No Hit CACCACTGAAGAGCATAAATCAGTCATCACAGATGTTCGTTTCAGGCCAA 5 0.125 No Hit ATTGCAGGTGGTGCCTTGTTAAGTACATTGTCAATTCGCCTTGGTTACAA 5 0.125 No Hit CAGGCAGCTGTTGCCATTGCTTAAACAAGTTGCCCGTTCTTGTGTCAATG 5 0.125 No Hit ACAAAATAGACAGGGCCAAAGTTGCTGGTGCTGCAGAAGATCTTCTTGAA 5 0.125 No Hit AGGCATTAAAGAGGGTGCAACTGTAAAAAATCAAGCTGTTTAAAGAAATA 5 0.125 No Hit CAAGGAGTTGGGTAATTTGAAAAACCTTGTTAGCATGGATATGTATGGCA 5 0.125 No Hit CAGCAATTTATGCATCTCAAACGGGTGCTTACTATGTTTTCTTGGTATCA 5 0.125 No Hit GGCACAAGAAATCTGGTCATCATCGACACAACCATGACAAGCACAAACAC 5 0.125 No Hit GGGAAAGAAGAAAACAAGAAGGATAAGGAGAAGAAAGAGAAAAAGCACAG 5 0.125 No Hit GATCCCTCAAAATAATTACGAGTTCACTGAGGTTGGAAAAGCACTCGATC 5 0.125 No Hit CCCAAACTATTTTGGCGAGATTCTCCTTTGGTGGGGTATTTTTGTGGCTT 5 0.125 No Hit TAATCTGTTTGATCTGTTTGTGTTACTATTTGTTACTGTTCACTGTTCTG 5 0.125 No Hit GGACCGATATCTCGGCCGATCTTTATTTCGAGTTGACCCAGGCCTTTAAG 5 0.125 No Hit GGAATTCAGAGCTGAGATTGTCATTTCTTCGCGACACAAGTGACAGAGTA 5 0.125 No Hit GAAGACCTCCTTGTAAACTTGCTAGCTGCAGTAAAATCAGTTGAAGCAAA 5 0.125 No Hit GTTGAAGGTGCAGACAATGAAAATGTTGGCTTTTATGGGAGATGTGCACT 5 0.125 No Hit GCCATTCTCCTCCTCCCCCAGGTCCGCATGCACCACCATCCGGTCCACCA 5 0.125 No Hit GATGTCGATATTTCAAGAACGTGGTGGGGTCTGATGGAATCCCGCCTAGT 5 0.125 No Hit GATTGTCTCACGGCAATGAACTTACTGGCACGATTCTGAATTCTGATCTA 5 0.125 No Hit CGTCACATGTGGAGAATTACACATCATCATCGTCCGACTGTGGGGCTGGA 5 0.125 No Hit ACTGATCTTTGCCGACGGTTTACATTTTTCGAAATCAACGAAGCAACAGG 5 0.125 No Hit GCTCATGGGGAGCAAGTATTTCCACGAGTTAAAGAAAAATGTTCTTCAAA 5 0.125 No Hit TCTCTCTAAACAATGGAATCGTTCGCTTCCTTCTTTGACTCTGAATCGTC 5 0.125 No Hit GCAACTTTCTTCCTGACTTTTGTGGCTCTGAAGTTTTTCGTTGGCTATGG 5 0.125 No Hit AGACCACTACTCTTCCTCTCTATAATTTTCTAGGGTTTAGCAATGTCTGC 5 0.125 No Hit GGCGGCTTTCGATTGGAATCATAAGCGAAAACTTGAAGCAATGAATGACT 5 0.125 No Hit GGATGTAACAGTGGAATTGGGGATCAGAGACTTGGTTTTTGTAGTTTGGG 5 0.125 No Hit GCCATTACAACTCAAACAACCCACATCAACCTCCTACAGAGCAGCCGTCC 5 0.125 No Hit TATCAATCCATAAAATATAGTCCTCGTGGTGTCATCCATTCATTAGAGCT 5 0.125 No Hit AAGGATGTTGAGGCTACTGGCATCCGAGAGATTATTGCTCTCCCAAAGAA 5 0.125 No Hit AGTCTAAGCATTTGTTCCTCACTGAATCTGTGAAGGTGTGGGTTAAACTC 5 0.125 No Hit GTCTGGTCACTCCATGTTTGTCTAATATAGTATTTGCTGTAAATTAAAGT 5 0.125 No Hit CACTCCCTCACAGGGGAAAGTCCGAAAGGACGGATGGTGGAATGATTGAG 5 0.125 No Hit CCACCTACTTCTTGCAGTGCAGGTCCTGTAGCTGAAGATATGTTTCACTG 5 0.125 No Hit CGGCAATGGGTTTTTTTCAGTCTGGATGTTGTCGTAAACTGTCGAATAAA 5 0.125 No Hit AATGAATCTATGGACGGACGACAACGGGTCTGTAATGGAAGCGTTTATGA 5 0.125 No Hit ATAATGAATTGGAGTCAACTCCCCCCAGCTCTTCATTGACGCCAACCACT 5 0.125 No Hit GAAGTGAATAAAGAGACGGTTGATCATGACGAACAATTTTCAATCCTGAA 5 0.125 No Hit GTTCATTCCTATCTCTCAATTTTGCCCTTTTACGTATGACTCCCTTAATA 5 0.125 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0 0.0 0.0 0.0 0.0 64-65 0.0 0.0 0.0 0.0 0.0 66-67 0.0 0.0 0.0 0.0 0.0 68-69 0.0 0.0 0.0 0.0 0.0 70-71 0.0 0.0 0.0 0.0 0.0 72-73 0.0 0.0 0.0 0.0 0.0 74-75 0.0125 0.0 0.0 0.0 0.0 76-77 0.05 0.0 0.0 0.0 0.0 78-79 0.0875 0.0 0.0 0.0 0.0 80-81 0.125 0.0 0.0 0.0 0.0 82-83 0.175 0.0 0.0 0.0 0.0 84-85 0.2375 0.0 0.0 0.0 0.0 86-87 0.275 0.0 0.0 0.0 0.0 88-89 0.3 0.0 0.0 0.0 0.0 90-91 0.4 0.0 0.0 0.0 0.0 92-93 0.5625 0.0 0.0 0.0 0.0 94-95 0.75 0.0 0.0 0.0 0.0 96-97 1.0 0.0 0.0 0.0 0.0 98-99 1.1 0.0 0.0 0.0 0.0 100-101 1.3125 0.0 0.0 0.0 0.0 102-103 1.575 0.0 0.0 0.0 0.0 104-105 1.85 0.0 0.0 0.0 0.0 106-107 2.0875000000000004 0.0 0.0 0.0 0.0 108-109 2.425 0.0 0.0 0.0 0.0 110-111 2.5625 0.0 0.0 0.0 0.0 112-113 2.925 0.0 0.0 0.0 0.0 114-115 3.25 0.0 0.0 0.0 0.0 116-117 3.7375 0.0 0.0 0.0 0.0 118-119 4.125 0.0 0.0 0.0 0.0 120-121 4.4375 0.0 0.0 0.0 0.0 122-123 5.0375 0.0 0.0 0.0 0.0 124-125 5.5875 0.0 0.0 0.0 0.0 126-127 6.2875 0.0 0.0 0.0 0.0 128-129 7.15 0.0 0.0 0.0 0.0 130-131 7.6625 0.0 0.0 0.0 0.0 132-133 8.075 0.0 0.0 0.0 0.0 134-135 8.3875 0.0 0.0 0.0 0.0 136-137 8.9375 0.0 0.0 0.0 0.0 138-139 9.75 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ACGTTCT 10 0.006830828 145.0 7 TAAGACG 10 0.006830828 145.0 3 CATTTGT 10 0.006830828 145.0 9 GACGTTC 10 0.006830828 145.0 6 TCTAAGC 10 0.006830828 145.0 3 AGACGTT 10 0.006830828 145.0 5 AAGACGT 10 0.006830828 145.0 4 AACTATA 10 0.006830828 145.0 145 GTCTAAG 10 0.006830828 145.0 2 TTTGGCC 10 0.006830828 145.0 8 AGTCTAA 10 0.006830828 145.0 1 AAGCATT 10 0.006830828 145.0 6 CGTTCTT 10 0.006830828 145.0 8 >>END_MODULE Read 1768816 spots for SRR26075351.sra Written 1768816 spots for SRR26075351.sra Read 1768816 spots for SRR26075351.sra Written 1768816 spots for SRR26075351.sra Read 1768816 spots for SRR26075351.sra Written 1768816 spots for SRR26075351.sra Read 1768816 spots for SRR26075351.sra Written 1768816 spots for SRR26075351.sra Read 1768816 spots for SRR26075351.sra Written 1768816 spots for SRR26075351.sra Read 1768816 spots for SRR26075351.sra Written 1768816 spots for SRR26075351.sra Read 1768816 spots for SRR26075351.sra Written 1768816 spots for SRR26075351.sra Read 1768816 spots for SRR26075351.sra Written 1768816 spots for SRR26075351.sra Read 1768816 spots for SRR26075351.sra Written 1768816 spots for SRR26075351.sra Read 1768816 spots for SRR26075351.sra Written 1768816 spots for SRR26075351.sra Read 1768816 spots for SRR26075351.sra Written 1768816 spots for SRR26075351.sra Read 1768816 spots for SRR26075351.sra Written 1768816 spots for SRR26075351.sra Read 1768830 spots for SRR26075351.sra Written 1768830 spots for SRR26075351.sra Read 1768816 spots for SRR26075351.sra Written 1768816 spots for SRR26075351.sra Read 1768816 spots for SRR26075351.sra Written 1768816 spots for SRR26075351.sra Read 1768816 spots for SRR26075351.sra Written 1768816 spots for SRR26075351.sra Read 1768816 spots for SRR26075351.sra Written 1768816 spots for SRR26075351.sra Read 1768816 spots for SRR26075351.sra Written 1768816 spots for SRR26075351.sra Read 1768816 spots for SRR26075351.sra Written 1768816 spots for SRR26075351.sra Read 1768816 spots for SRR26075351.sra Written 1768816 spots for SRR26075351.sra SRR ids: ['SRR26075351.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_k66q4012 SRR26075351.sra spots: 35376334 blocks: [[1, 1768816], [1768817, 3537632], [3537633, 5306448], [5306449, 7075264], [7075265, 8844080], [8844081, 10612896], [10612897, 12381712], [12381713, 14150528], [14150529, 15919344], [15919345, 17688160], [17688161, 19456976], [19456977, 21225792], [21225793, 22994608], [22994609, 24763424], [24763425, 26532240], [26532241, 28301056], [28301057, 30069872], [30069873, 31838688], [31838689, 33607504], [33607505, 35376334]] SRR26075351 file size 13064062 SRR26075351 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o SRR26075351 SRR26075351_1.fastq SRR26075351_2.fastq Input file: SRR26075351_1.fastq Paired file: SRR26075351_2.fastq trimmed: SRR26075351-trimmed-pair1.fastq, SRR26075351-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Tue Feb 11 22:05:01 2025 >> started Tue Feb 11 22:05:40 2025 >> done (39.079s) 35376334 read pairs processed; of these: 130 ( 0.00%) short read pairs filtered out after trimming by size control 132563 ( 0.37%) empty read pairs filtered out after trimming by size control 35243641 (99.62%) read pairs available; of these: 5382985 (15.27%) trimmed read pairs available after processing 29860656 (84.73%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 18 14 0.00% 19 22 0.00% 20 21 0.00% 21 26 0.00% 22 29 0.00% 23 41 0.00% 24 37 0.00% 25 45 0.00% 26 39 0.00% 27 66 0.00% 28 60 0.00% 29 60 0.00% 30 63 0.00% 31 53 0.00% 32 68 0.00% 33 84 0.00% 34 93 0.00% 35 78 0.00% 36 66 0.00% 37 88 0.00% 38 111 0.00% 39 131 0.00% 40 105 0.00% 41 135 0.00% 42 128 0.00% 43 156 0.00% 44 175 0.00% 45 156 0.00% 46 181 0.00% 47 245 0.00% 48 277 0.00% 49 290 0.00% 50 311 0.00% 51 346 0.00% 52 394 0.00% 53 472 0.00% 54 526 0.00% 55 535 0.00% 56 545 0.00% 57 720 0.00% 58 752 0.00% 59 803 0.00% 60 1062 0.00% 61 1127 0.00% 62 1294 0.00% 63 1442 0.00% 64 1754 0.00% 65 1604 0.00% 66 2007 0.01% 67 2284 0.01% 68 2520 0.01% 69 2948 0.01% 70 3395 0.01% 71 4056 0.01% 72 4474 0.01% 73 5280 0.01% 74 5459 0.02% 75 6389 0.02% 76 6926 0.02% 77 7821 0.02% 78 8462 0.02% 79 9140 0.03% 80 10969 0.03% 81 11672 0.03% 82 13537 0.04% 83 14631 0.04% 84 16349 0.05% 85 17814 0.05% 86 18465 0.05% 87 19944 0.06% 88 20835 0.06% 89 22268 0.06% 90 24331 0.07% 91 25812 0.07% 92 28777 0.08% 93 30327 0.09% 94 32910 0.09% 95 35026 0.10% 96 36238 0.10% 97 37572 0.11% 98 38966 0.11% 99 40532 0.12% 100 42384 0.12% 101 43724 0.12% 102 47360 0.13% 103 49251 0.14% 104 52193 0.15% 105 54179 0.15% 106 56372 0.16% 107 57632 0.16% 108 57983 0.16% 109 60514 0.17% 110 61418 0.17% 111 64415 0.18% 112 67406 0.19% 113 68719 0.19% 114 72430 0.21% 115 75431 0.21% 116 77283 0.22% 117 79097 0.22% 118 80662 0.23% 119 81002 0.23% 120 82630 0.23% 121 84160 0.24% 122 86521 0.25% 123 90004 0.26% 124 92415 0.26% 125 95208 0.27% 126 97970 0.28% 127 99170 0.28% 128 101839 0.29% 129 103694 0.29% 130 103475 0.29% 131 105677 0.30% 132 106252 0.30% 133 110836 0.31% 134 112736 0.32% 135 115624 0.33% 136 119916 0.34% 137 119811 0.34% 138 121821 0.35% 139 123637 0.35% 140 122617 0.35% 141 125674 0.36% 142 126949 0.36% 143 129573 0.37% 144 133199 0.38% 145 136370 0.39% 146 137138 0.39% 147 138073 0.39% 148 141102 0.40% 149 142091 0.40% 150 142382 0.40% 151 29860656 84.73% 35243641 reads passed initial QC criterion=sequence-density sequence-density=0.26 sequence-density-rank=1 fanout-score=19.32 fanout-score-rank=5 prefix-density=0.22 prefix-fanout=19.3 sequence=GGATCGGAAGAGCACACGTCTGAACTCCAGTCACAACGGTCAATCTCGTATGCCGTCTTCTGCTTG criterion=fanout-score sequence-density=0.05 sequence-density-rank=34 fanout-score=60.54 fanout-score-rank=1 prefix-density=0.27 prefix-fanout=12.0 sequence=TTCTTCACAATGCCAGCCTGAACAAGGAAGGTCGATACATTCTTGCGCTGGTCACCTTGAAGTTGAATAACCTGGCCTAATTCAGGGTCCTGCACCACTGTACCATTACAGCAGAACTCTTTCTTGAGGTCCTTTAGTATCTTGTTATAGCTGAATTCTTTTTTCAAACCTTGCACAGTTGTCAAGCTTTTCCTACCATTGCGTTGCTGTATACGAATGTGCACATAATCTTTTGTCCCAGCACCAGAGTCCTCGGCATTTGCATCAGCAAAAGGATCATAAGCTGAAGGAGTTTGGGCGTCGAAATCAGACATGAAAACTTAACTGTTCAAGGAAGTCCAACAACCTGAAAGCTCAGACCTTATCCGGTGATTAGGGTTTCGACGCGTTAGATTATTTGATTGATATGAAGCGAAACCCTAAATCGGATGAGTCTAAGCTTTTCTGATG criterion=sequence-density sequence-density=0.21 sequence-density-rank=1 fanout-score=2.79 fanout-score-rank=35 prefix-density=0.22 prefix-fanout=2.8 sequence=ATGTACCCTGACTTAGGTTTCTCAGA criterion=fanout-score sequence-density=0.01 sequence-density-rank=43 fanout-score=289.62 fanout-score-rank=1 prefix-density=0.13 prefix-fanout=11.8 sequence=AGAGAAAAGATAAGCTAGGCAAGATGGTTTTACTAGACAAGATGTGGGATGATGTTGTTGCTGGACCTCAGCCAGAACGTGGCCTTGGCAAGCTTAGAAAGATCAGCACCAGACCACTTAACATCAAAGATATTGACGTCGGAGAGGGGAGCAGTCCTGTTAATAAGTTTCAGAGGTCCATGACTATGCCAGGAACTCCAGGGACACCGACGACACCAGTGACCCCTACAACCCCAGTGTCGGCGCGTAGCAATGTTTGGAGGAGCGTGTTCCACCCTGGTAGCAACCTTGCTACTAAGAATATTGGTGCTCATGTTTTTGACAAGCCACAGCCTAACACACCCACTGTCTATGACTGGATGTACAGTGGAGAGACGAAGAGCGAGCATCGTTGATGAGGTTGCCTTCAACCAAGGTTGCCCATGTAAATACGTACTGTGTTTTGTTTTTCAGTACT SRR26075351 testing PE reads STAR mapping to Ensembl genome Started job on | Feb 11 22:06:23 Started mapping on | Feb 11 22:06:23 Finished on | Feb 11 22:11:39 Mapping speed, Million of reads per hour | 401.51 Number of input reads | 35243641 Average input read length | 294 UNIQUE READS: Uniquely mapped reads number | 30974040 Uniquely mapped reads % | 87.89% Average mapped length | 292.10 Number of splices: Total | 26948926 Number of splices: Annotated (sjdb) | 26280598 Number of splices: GT/AG | 26474208 Number of splices: GC/AG | 356150 Number of splices: AT/AC | 27229 Number of splices: Non-canonical | 91339 Mismatch rate per base, % | 0.41% Deletion rate per base | 0.03% Deletion average length | 3.34 Insertion rate per base | 0.02% Insertion average length | 2.70 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 985872 % of reads mapped to multiple loci | 2.80% Number of reads mapped to too many loci | 179601 % of reads mapped to too many loci | 0.51% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 8.14% % of reads unmapped: other | 0.67% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 3283729 3283729 3283729 N_multimapping 985872 985872 985872 N_noFeature 707589 30523703 1010278 N_ambiguous 351043 2704 201410 UnstrandedReadsAssigned:29915408 PositiveStrandReadsAssigned:447633 NegativeStrandReadsAssigned:29762352 Dataset is classified negative stranded MeadianReadLen=151 20thPercentileLength=151 echo kmer=147 SRR26075351 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,400 [index] number of k-mers: 62,057,036 [index] number of equivalence classes: 130,681 [quant] running in paired-end mode [quant] will process pair 1: SRR26075351-trimmed-pair1.fastq SRR26075351-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 35,243,641 reads, 30,600,405 reads pseudoaligned [quant] estimated average fragment length: 214.865 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,221 rounds 52401 SRR26075351.ke.tsv 34699 SRR26075351.se.tsv 87100 total ==> SRR26075351.ke.tsv <== target_id length eff_length est_counts tpm Potri.005G200100.1.v4.1 2018 1804.14 6291 101.987 Potri.005G024800.1.v4.1 1035 821.135 3625 129.118 Potri.004G059700.1.v4.1 961 747.142 72 2.81852 Potri.007G009000.2.v4.1 1416 1202.14 0 0 Potri.003G141000.2.v4.1 2943 2729.14 1533 16.4289 Potri.016G087400.1.v4.1 270 90.3865 2774 897.625 Potri.015G069301.1.v4.1 564 352.979 0 0 Potri.010G195200.1.v4.1 1773 1559.14 162 3.03895 Potri.012G127500.1.v4.1 977 763.142 13951 534.678 ==> SRR26075351.se.tsv <== Potri.001G166300.v4.1 0 Potri.001G448400.v4.1 754 Potri.001G233950.v4.1 0 Potri.001G122700.v4.1 278 Potri.001G212900.v4.1 0 Potri.001G182400.v4.1 0 Potri.001G256600.v4.1 0 Potri.001G040500.v4.1 3 Potri.001G416900.v4.1 0 Potri.001G452600.v4.1 895 SRR26075351 completed mapping pipeline successfully