Starting /dee2/code/volunteer_pipeline.sh SRR26075352 current disk space = 3052633223168 free memory = 1462793176 SRR26075352 SRAfilesize cd48e0918d82db2ab9956a8954024fd0 SRR26075352.sra SRR26075352.sra file validated SRR26075352 is paired end SRR26075352 is conventional basespace SRR26075352 read1 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR26075352_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.469 37.0 37.0 37.0 37.0 37.0 2 36.52 37.0 37.0 37.0 37.0 37.0 3 36.6585 37.0 37.0 37.0 37.0 37.0 4 36.687 37.0 37.0 37.0 37.0 37.0 5 36.7405 37.0 37.0 37.0 37.0 37.0 6 36.62 37.0 37.0 37.0 37.0 37.0 7 36.656 37.0 37.0 37.0 37.0 37.0 8 36.6455 37.0 37.0 37.0 37.0 37.0 9 36.699 37.0 37.0 37.0 37.0 37.0 10-14 36.66555 37.0 37.0 37.0 37.0 37.0 15-19 36.61450000000001 37.0 37.0 37.0 37.0 37.0 20-24 36.5471 37.0 37.0 37.0 37.0 37.0 25-29 36.5084 37.0 37.0 37.0 37.0 37.0 30-34 36.3938 37.0 37.0 37.0 37.0 37.0 35-39 36.339999999999996 37.0 37.0 37.0 37.0 37.0 40-44 36.3752 37.0 37.0 37.0 37.0 37.0 45-49 36.3189 37.0 37.0 37.0 37.0 37.0 50-54 36.2143 37.0 37.0 37.0 37.0 37.0 55-59 36.2278 37.0 37.0 37.0 37.0 37.0 60-64 36.16330000000001 37.0 37.0 37.0 37.0 37.0 65-69 36.07469999999999 37.0 37.0 37.0 37.0 37.0 70-74 36.1201 37.0 37.0 37.0 37.0 37.0 75-79 36.048899999999996 37.0 37.0 37.0 37.0 37.0 80-84 35.8983 37.0 37.0 37.0 37.0 37.0 85-89 35.9096 37.0 37.0 37.0 37.0 37.0 90-94 35.8583 37.0 37.0 37.0 37.0 37.0 95-99 35.861599999999996 37.0 37.0 37.0 37.0 37.0 100-104 35.6991 37.0 37.0 37.0 37.0 37.0 105-109 35.7433 37.0 37.0 37.0 37.0 37.0 110-114 35.5989 37.0 37.0 37.0 37.0 37.0 115-119 35.4853 37.0 37.0 37.0 34.6 37.0 120-124 35.5637 37.0 37.0 37.0 37.0 37.0 125-129 35.508399999999995 37.0 37.0 37.0 37.0 37.0 130-134 35.31999999999999 37.0 37.0 37.0 32.2 37.0 135-139 35.156600000000005 37.0 37.0 37.0 29.8 37.0 140-144 35.0056 37.0 37.0 37.0 25.0 37.0 145-149 34.9901 37.0 37.0 37.0 25.0 37.0 150-151 34.8245 37.0 37.0 37.0 25.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.0 1101 2 0.0 1101 3 0.0 1101 4 0.0 1101 5 0.0 1101 6 0.0 1101 7 0.0 1101 8 0.0 1101 9 0.0 1101 10-14 0.0 1101 15-19 0.0 1101 20-24 0.0 1101 25-29 0.0 1101 30-34 0.0 1101 35-39 0.0 1101 40-44 0.0 1101 45-49 0.0 1101 50-54 0.0 1101 55-59 0.0 1101 60-64 0.0 1101 65-69 0.0 1101 70-74 0.0 1101 75-79 0.0 1101 80-84 0.0 1101 85-89 0.0 1101 90-94 0.0 1101 95-99 0.0 1101 100-104 0.0 1101 105-109 0.0 1101 110-114 0.0 1101 115-119 0.0 1101 120-124 0.0 1101 125-129 0.0 1101 130-134 0.0 1101 135-139 0.0 1101 140-144 0.0 1101 145-149 0.0 1101 150-151 0.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 17 1.0 18 0.0 19 0.0 20 0.0 21 1.0 22 1.0 23 4.0 24 4.0 25 9.0 26 7.0 27 9.0 28 22.0 29 16.0 30 33.0 31 49.0 32 67.0 33 108.0 34 184.0 35 500.0 36 2801.0 37 184.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 35.0828729281768 13.937719738824711 9.040683073832245 41.93872425916625 2 18.099999999999998 13.750000000000002 37.275000000000006 30.875000000000004 3 18.0 15.575 28.599999999999998 37.824999999999996 4 22.35 25.2 23.875 28.575 5 23.35 30.55 26.3 19.8 6 20.65 32.824999999999996 23.25 23.275000000000002 7 14.825 28.9 39.425 16.85 8 16.725 27.05 32.75 23.474999999999998 9 18.625 24.55 32.725 24.099999999999998 10-14 19.940997049852495 29.071453572678635 28.116405820291014 22.87114355717786 15-19 19.91 27.3 28.865000000000002 23.925 20-24 19.81 27.965 28.655 23.57 25-29 19.905 27.755000000000003 27.634999999999998 24.705 30-34 19.35 28.28 28.155 24.215 35-39 19.400000000000002 28.025 28.050000000000004 24.525 40-44 20.375 27.115000000000002 28.88 23.630000000000003 45-49 20.990000000000002 28.01 27.08 23.919999999999998 50-54 20.205000000000002 29.07 27.560000000000002 23.165 55-59 19.82 28.275 27.63 24.275 60-64 19.7 28.535 27.42 24.345 65-69 20.05 28.155 27.495000000000005 24.3 70-74 19.805 28.349999999999998 27.810000000000002 24.035 75-79 19.875 28.16 27.965 24.0 80-84 20.445 27.744999999999997 28.134999999999998 23.674999999999997 85-89 20.345 28.03 28.24 23.385 90-94 20.875 27.775 27.73 23.62 95-99 21.39 27.63 27.839999999999996 23.14 100-104 20.990000000000002 27.96 27.965 23.085 105-109 20.415 28.32 27.73 23.535 110-114 20.445 27.779999999999998 27.26 24.515 115-119 20.595 28.035 27.334999999999997 24.035 120-124 21.285 28.835 26.314999999999998 23.565 125-129 20.94 28.299999999999997 26.41 24.349999999999998 130-134 21.965 27.54 26.415 24.08 135-139 21.15 27.775 27.060000000000002 24.015 140-144 20.810000000000002 27.950000000000003 26.625 24.615000000000002 145-149 21.51 27.625 26.995 23.87 150-151 21.6125 28.549999999999997 25.35 24.4875 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.5 16 0.5 17 0.0 18 0.0 19 0.0 20 2.0 21 2.5 22 4.5 23 4.0 24 0.0 25 0.5 26 5.0 27 8.0 28 8.5 29 14.0 30 20.5 31 18.5 32 24.0 33 32.5 34 44.0 35 62.0 36 79.0 37 101.5 38 113.0 39 143.5 40 191.0 41 226.0 42 268.5 43 276.0 44 269.5 45 276.0 46 267.0 47 260.0 48 245.0 49 205.5 50 156.0 51 141.5 52 133.5 53 102.0 54 73.0 55 54.0 56 38.5 57 26.0 58 21.0 59 15.0 60 12.0 61 9.0 62 6.5 63 8.5 64 7.5 65 6.0 66 3.5 67 3.0 68 4.5 69 3.0 70 1.0 71 0.5 72 0.5 73 0.5 74 0.5 75 0.0 76 0.0 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.44999999999999996 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.005 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150-151 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 60.824999999999996 #Duplication Level Percentage of deduplicated Percentage of total 1 63.13193588162762 38.4 2 21.783806000822032 26.5 3 7.93259350595972 14.475 4 4.315659679408138 10.5 5 1.4385532264693794 4.375 6 0.8631319358816275 3.15 7 0.2877106452938759 1.225 8 0.04110152075626798 0.2 9 0.16440608302507193 0.8999999999999999 >10 0.04110152075626798 0.27499999999999997 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source ATCTGTTCAGTCCTCTTACGAATGGCGTTCTCAGCATAGATCCGAGCACC 11 0.27499999999999997 No Hit GTGGATTTCTAAATGTTGGTTTACACAACTATACATGTTAGTCGCCGTAT 9 0.22499999999999998 No Hit GTCGATCATCTTCGTTTGATATGGTGCCCATGTTATGTGCTCGAATGATA 9 0.22499999999999998 No Hit CAGTCTTGTTCCTCTCGTGAGCCAAGTGATCCACTCCTTTCATTTTCTTC 9 0.22499999999999998 No Hit CCCTAAGCGAAGGTGTGCGATTTTGCAGTTGCTTCTCTCGATTTCAGCAA 9 0.22499999999999998 No Hit CCTCTCACAGTTATCAATTTATGCGCCATTCTTGCTCGAAAAACAAGTGG 8 0.2 No Hit CTGAACATCTCCTGGATGGAGGTTGAGTTTCCAATGAAGGTGGATGCCAT 7 0.17500000000000002 No Hit AGGTAATGCAAGGACGTCTTCTTTCTAGGACATACTGCATTCCGGAGGTA 7 0.17500000000000002 No Hit TCTTGCTCAGGGATGCCCACTTTCACCTGCACAAACATACACTGAGCCGG 7 0.17500000000000002 No Hit CTCTGATATCATAATATTTCTCATCCACTTAACTAAACTTAATTCACGAG 7 0.17500000000000002 No Hit TGTTGGCTTTGGCTTTGGCTTTGGCTTTGGTTTTGGTTTTGGAGACGGGC 7 0.17500000000000002 No Hit TGTGGAAACTTTACCCAATCCTCCAGGCCACCATTTGGATGAAGTTAATG 7 0.17500000000000002 No Hit TGCACCACCAGCCATACGATAAAAACCTCAAACACTAAATCTCTAGAATA 7 0.17500000000000002 No Hit CCCGAATCAGCGACCTTTTACCCGATCTGGATCTAAAAGCAAAACCACCA 6 0.15 No Hit CGAGGGATAAAAATAAACACTTTTGCAGCAAATTAAAATTTCTTCCCCGG 6 0.15 No Hit CCCAAGATTCCTTTTCCTTGGTGAAGGAGATCATCTCAACTTCATAGTAC 6 0.15 No Hit ATCTTCTTCATGTTTTAGGTTCGCGTTTTACGAGCAAACTTTCTCTGCTT 6 0.15 No Hit CGCCACGTTTGGCTTCAACCATGGCCCTCTGTTCTTCAGCATCTTTATGA 6 0.15 No Hit GTGGAAAGGGTAAATGGGCTTACTGTTCGTGGTCCGACTGCTTCACAACA 6 0.15 No Hit CTCATCTTCACCCTCTTTTATCTCCCATGGTGTCCTTCCTCTGCTGTACC 6 0.15 No Hit AGTAGCCACAGTGACATAAAGGAAGAGAAGTGTAGCAATGAACTCAGCTA 6 0.15 No Hit GTTCGATTTCTACTCCGAAAGCTGCCGTCGGAGCTGGGATTGTTTACCTT 6 0.15 No Hit CCTCAATGAGAAGAATGGAACGATTGGTGGTGGAGGTAAGTATCCTCCTC 6 0.15 No Hit GTTGCTGGCAGATGTATAAACATGATAATCATAGTAATCAGCTGGGTGAT 6 0.15 No Hit CTGAAATCCCTGCAAGCAATCACAATTCTCTGCTTCTTTTCGGACAACAT 6 0.15 No Hit GTATACAATCATCTTCGTCTTCTTCACGAGCAGCCGGTATCTATGCCAGG 6 0.15 No Hit TCCTGAAAGAACTGGTTCCTGCATGATAATCAAGACATCCATGGGATCAT 6 0.15 No Hit ATTCTCTTTGCTGGTGCCCAAATAATGATAAGGAATACTATTCCTTTCAA 6 0.15 No Hit CGTTGCTGTCACCATTTTGGTTTCTTCACTTGAGGATTTTTGTTTTATAG 6 0.15 No Hit GGCTCCTTCAATCTGAGTAAACTCTGAACAATGAGATCTTTGAGAAGATG 6 0.15 No Hit GTTGATCTAGTAAAAGTATTCCTCTCTCTCTCCCCTACACGATAATAGGA 6 0.15 No Hit CTGACAAATCGGCAACATATTTGGCCAAGGAAACAGCCACATCCTGTTCC 6 0.15 No Hit CACCGATCATGGCTGTGACAGAGTGGAAAGTTGAGTACCACCATTTGGCT 6 0.15 No Hit CAGCAAATGAAATCCTACAGAGGATTTTGAGGAACCCTTTGCTGTTTTAT 6 0.15 No Hit CTCACAGCATCATCAAATTTTACATTAAATCAAAATTTTGCACACAAATG 5 0.125 No Hit TGACTTTCATTCCCCTTCTAAGCCTTACTATGCTGCTGCAGCTCCCTTGT 5 0.125 No Hit CCGGGACTTCAGGATGGAGTTTTTCGTATTCAATGGTCAGTTTTGCCAAG 5 0.125 No Hit CTGAAAACCTTTTTCTCATAACCTCATTACTCAGTCTTTTCTACTGGGGT 5 0.125 No Hit GAGACAGTGGCCACATCGTGTGCACCTGTAGTGAATGTGAATTCTGCCAA 5 0.125 No Hit GCTTCGTCTCTCTGCAGATGCTCTTTTGGAAATACTTGCCCTTCAACCAA 5 0.125 No Hit GATCCATTGATAATCTCGTCTCTGCCAAAGCAACTGCCTTTTGTGCTAAT 5 0.125 No Hit GGCTAGAAAGACAAGACAGAAAGCAATTCCCTTGAGCTGATGACAATTTG 5 0.125 No Hit ACCGTACTGGCTAAAAGCCTCTTGGAGGACTTGGTCAGTGGTGGCCCAAG 5 0.125 No Hit GGGTCTACGAGGATGGACCTCACATACTCTAGCAAATTGATTTTATACAT 5 0.125 No Hit CTTGGGAATGCACAGCTTTGTCTCCAGAATTTCTGCAATTGCAGCACTGA 5 0.125 No Hit GTTGGCAGAGTTGAGGCACTCATTGTCATGGACACGCAAGTCATTGCGAA 5 0.125 No Hit GACATCTTCTCCGAGGATTGTTAAGTTCTCTACACGGGCCCATTGCCCAA 5 0.125 No Hit CTCTAATTTCCTGAAGTTTTGCTTTCCACCACTCATTGCTAGCTAAATTT 5 0.125 No Hit TGAAATTTCTATAAGAAGCGGTGAAAGGAGCCAGGGCCCAATCTGTCTTG 5 0.125 No Hit CAAAGATGGTTATGCCAAGCTTTCTATGGGTGTTGTAGGTGATTCCAGGA 5 0.125 No Hit TCTGCATCTTTATGCTGGGAAAACCACCTCCTGATGTTGGAGCTCGAATA 5 0.125 No Hit CATTCAAGCACAAGTAGTAATATCTGTTGGCACCAGTTGGTCTAAATATC 5 0.125 No Hit GGCCTCCAGATAAAAGAACTCCAAAAGGCACATCAGTCATTAGCCTTTTA 5 0.125 No Hit GCAACCAAAACATTCTCAGAATCATTCAGCTCTCCCTTGGAATGTTCAGT 5 0.125 No Hit CCTCTCATAAATGGCCTTTGGTACATTGGCATTCCTTCATCATTTATAGC 5 0.125 No Hit GTTGTTGTTTAGCTCCTTAATCAAACCAACATTTCGAGTCAAGTTGTCAG 5 0.125 No Hit TTTTTTTTTTGTTTTAAAATATATTAAAATAATATCAATTTAGCAGGTTT 5 0.125 No Hit CTCAACAACACCATCATCTCTTGGCCTAGTATCCAACATCTTACCATGAA 5 0.125 No Hit GTCAGGGTCTTGCGCCAAGCAAGCATCGAGTACGGCATCAGAGATCTGGT 5 0.125 No Hit GCTAGCTAGACAAGAACAGAACTGCAAAAATCAAAGCCATAAGGCTCCAG 5 0.125 No Hit TCAGTGTCAAGATTACAGCACCAGCTGCAAGCAGTGCAAATGCAACAGCA 5 0.125 No Hit GTTTTTTGTAATCCCTTTGAAAATATTTAATTCATCTGGCTTCCGGGCAT 5 0.125 No Hit CAGGGCTATAGAGTCCAGAGTCTTGCAGAGCCCTTTCGTTGACATCAGAG 5 0.125 No Hit CACCACCTCAATATCCGGGCACTTGAGTGCAATGACAGCCATTGTAGGCC 5 0.125 No Hit CTCAGATCGGGTACTCCAGCAGCCTTGAACGCCTCGTCGTTTGGAGCAAA 5 0.125 No Hit CTCCGTCACCCTCTCCAGCTCCTTAACGGCACCGTTTAGGCTCTCCCCTT 5 0.125 No Hit CTGTATTCTTGATTGTCCCAACCTTTTCATATTTGCTCATGAACTTGTAT 5 0.125 No Hit GTCCTGCAAAGGTGAGAGCTGGTCCAATTGGAACTGATCTTGTGAAGCAA 5 0.125 No Hit ATCATCATTGTCCTCAACTCACTTAATCGACTTCCTCGATCTTAGGACCT 5 0.125 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0125 0.0 0.0 0.0 0.0 52-53 0.025 0.0 0.0 0.0 0.0 54-55 0.025 0.0 0.0 0.0 0.0 56-57 0.025 0.0 0.0 0.0 0.0 58-59 0.05 0.0 0.0 0.0 0.0 60-61 0.05 0.0 0.0 0.0 0.0 62-63 0.05 0.0 0.0 0.0 0.0 64-65 0.05 0.0 0.0 0.0 0.0 66-67 0.05 0.0 0.0 0.0 0.0 68-69 0.0625 0.0 0.0 0.0 0.0 70-71 0.075 0.0 0.0 0.0 0.0 72-73 0.075 0.025 0.0 0.0 0.0 74-75 0.0875 0.05 0.0 0.0 0.0 76-77 0.15 0.05 0.0 0.0 0.0 78-79 0.1875 0.05 0.0 0.0 0.0 80-81 0.2375 0.05 0.0 0.0 0.0 82-83 0.3375 0.05 0.0 0.0 0.0 84-85 0.4625 0.05 0.0 0.0 0.0 86-87 0.5875 0.05 0.0 0.0 0.0 88-89 0.65 0.05 0.0 0.0 0.0 90-91 0.8875 0.05 0.0 0.0 0.0 92-93 1.2 0.05 0.0 0.0 0.0 94-95 1.375 0.05 0.0 0.0 0.0 96-97 1.6124999999999998 0.05 0.0 0.0 0.0 98-99 1.9375 0.05 0.0 0.0 0.0 100-101 2.225 0.05 0.0 0.0 0.0 102-103 2.5 0.05 0.0 0.0 0.0 104-105 2.925 0.05 0.0 0.0 0.0 106-107 3.3375 0.05 0.0 0.0 0.0 108-109 3.6500000000000004 0.05 0.0 0.0 0.0 110-111 4.025 0.05 0.0 0.0 0.0 112-113 4.525 0.05 0.0 0.0 0.0 114-115 5.125 0.05 0.0 0.0 0.0 116-117 5.5375 0.05 0.0 0.0 0.0 118-119 6.1625 0.05 0.0 0.0 0.0 120-121 6.699999999999999 0.05 0.0 0.0 0.0 122-123 7.25 0.05 0.0 0.0 0.0 124-125 7.9 0.05 0.0 0.0 0.0 126-127 8.4375 0.05 0.0 0.0 0.0 128-129 9.05 0.05 0.0 0.0 0.0 130-131 9.5875 0.05 0.0 0.0 0.0 132-133 10.462499999999999 0.05 0.0 0.0 0.0 134-135 10.9875 0.05 0.0 0.0 0.0 136-137 11.875 0.05 0.0 0.0 0.0 138-139 12.45 0.05 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CAATGTA 10 0.006830828 145.0 4 TATATGG 10 0.006830828 145.0 145 TCAATGT 10 0.006830828 145.0 3 TAGCACT 10 0.006830828 145.0 9 AATGTAG 10 0.006830828 145.0 5 >>END_MODULE SRR26075352 read2 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR26075352_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.2245 37.0 37.0 37.0 37.0 37.0 2 36.269 37.0 37.0 37.0 37.0 37.0 3 36.2795 37.0 37.0 37.0 37.0 37.0 4 36.39 37.0 37.0 37.0 37.0 37.0 5 36.345 37.0 37.0 37.0 37.0 37.0 6 36.333 37.0 37.0 37.0 37.0 37.0 7 36.326 37.0 37.0 37.0 37.0 37.0 8 36.4015 37.0 37.0 37.0 37.0 37.0 9 36.48 37.0 37.0 37.0 37.0 37.0 10-14 36.4028 37.0 37.0 37.0 37.0 37.0 15-19 36.3917 37.0 37.0 37.0 37.0 37.0 20-24 36.3461 37.0 37.0 37.0 37.0 37.0 25-29 36.293600000000005 37.0 37.0 37.0 37.0 37.0 30-34 36.2 37.0 37.0 37.0 37.0 37.0 35-39 36.1618 37.0 37.0 37.0 37.0 37.0 40-44 36.09310000000001 37.0 37.0 37.0 37.0 37.0 45-49 36.0852 37.0 37.0 37.0 37.0 37.0 50-54 35.9856 37.0 37.0 37.0 37.0 37.0 55-59 35.97879999999999 37.0 37.0 37.0 37.0 37.0 60-64 36.0298 37.0 37.0 37.0 37.0 37.0 65-69 35.964299999999994 37.0 37.0 37.0 37.0 37.0 70-74 35.930899999999994 37.0 37.0 37.0 37.0 37.0 75-79 35.8074 37.0 37.0 37.0 37.0 37.0 80-84 35.8749 37.0 37.0 37.0 37.0 37.0 85-89 35.81339999999999 37.0 37.0 37.0 37.0 37.0 90-94 35.7254 37.0 37.0 37.0 37.0 37.0 95-99 35.768600000000006 37.0 37.0 37.0 37.0 37.0 100-104 35.636 37.0 37.0 37.0 37.0 37.0 105-109 35.5781 37.0 37.0 37.0 37.0 37.0 110-114 35.5393 37.0 37.0 37.0 37.0 37.0 115-119 35.5269 37.0 37.0 37.0 37.0 37.0 120-124 35.349599999999995 37.0 37.0 37.0 34.6 37.0 125-129 35.4015 37.0 37.0 37.0 37.0 37.0 130-134 35.4594 37.0 37.0 37.0 37.0 37.0 135-139 35.150600000000004 37.0 37.0 37.0 29.8 37.0 140-144 35.2995 37.0 37.0 37.0 29.8 37.0 145-149 35.162299999999995 37.0 37.0 37.0 27.4 37.0 150-151 34.7325 37.0 37.0 37.0 25.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.0 1101 2 0.0 1101 3 0.0 1101 4 0.0 1101 5 0.0 1101 6 0.0 1101 7 0.0 1101 8 0.0 1101 9 0.0 1101 10-14 0.0 1101 15-19 0.0 1101 20-24 0.0 1101 25-29 0.0 1101 30-34 0.0 1101 35-39 0.0 1101 40-44 0.0 1101 45-49 0.0 1101 50-54 0.0 1101 55-59 0.0 1101 60-64 0.0 1101 65-69 0.0 1101 70-74 0.0 1101 75-79 0.0 1101 80-84 0.0 1101 85-89 0.0 1101 90-94 0.0 1101 95-99 0.0 1101 100-104 0.0 1101 105-109 0.0 1101 110-114 0.0 1101 115-119 0.0 1101 120-124 0.0 1101 125-129 0.0 1101 130-134 0.0 1101 135-139 0.0 1101 140-144 0.0 1101 145-149 0.0 1101 150-151 0.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 13 1.0 14 1.0 15 0.0 16 1.0 17 0.0 18 0.0 19 1.0 20 3.0 21 6.0 22 7.0 23 1.0 24 8.0 25 13.0 26 7.0 27 13.0 28 16.0 29 17.0 30 24.0 31 40.0 32 60.0 33 90.0 34 207.0 35 676.0 36 2590.0 37 218.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 39.900000000000006 20.825 11.774999999999999 27.500000000000004 2 26.25 25.575 31.7 16.475 3 19.5 27.750000000000004 33.2 19.55 4 25.650000000000002 32.225 24.45 17.675 5 26.174999999999997 34.525 21.875 17.424999999999997 6 20.375 39.675 21.825 18.125 7 20.549999999999997 22.7 38.475 18.275 8 20.349999999999998 26.075 29.825000000000003 23.75 9 25.174999999999997 22.925 28.975 22.925 10-14 23.485 29.225 26.924999999999997 20.365 15-19 22.95 29.110000000000003 27.345000000000002 20.595 20-24 23.31 28.65 27.955000000000002 20.085 25-29 23.34 28.075 27.66 20.925 30-34 23.294999999999998 28.4 27.85 20.455000000000002 35-39 23.630000000000003 27.395000000000003 27.839999999999996 21.135 40-44 23.69 28.265 28.055000000000003 19.99 45-49 24.52 27.935 27.52 20.025000000000002 50-54 22.650000000000002 29.17 27.525 20.655 55-59 23.235 28.01 28.23 20.525 60-64 24.04 27.82 27.339999999999996 20.8 65-69 23.575 28.465 27.485 20.474999999999998 70-74 23.494999999999997 28.895 27.150000000000002 20.46 75-79 23.585 28.155 27.12 21.14 80-84 23.485 28.794999999999998 26.76 20.96 85-89 23.1 28.53 26.995 21.375 90-94 23.62 28.82 26.825 20.735 95-99 24.215 29.975 25.869999999999997 19.939999999999998 100-104 24.560000000000002 28.73 27.29 19.42 105-109 24.85 28.09 27.365000000000002 19.695 110-114 24.375 28.675 27.18 19.77 115-119 24.575 28.42 26.82 20.185 120-124 24.325 29.285 26.700000000000003 19.689999999999998 125-129 24.995 28.13 26.650000000000002 20.225 130-134 25.91 28.494999999999997 26.119999999999997 19.475 135-139 25.900000000000002 28.03 26.08 19.99 140-144 26.36 28.34 26.545 18.755 145-149 27.165 28.485 25.805 18.545 150-151 26.724999999999998 27.975 26.474999999999998 18.825 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.5 5 0.5 6 0.5 7 1.0 8 1.0 9 0.5 10 1.5 11 1.5 12 0.0 13 0.5 14 1.0 15 1.0 16 1.0 17 1.5 18 1.0 19 0.5 20 1.0 21 0.5 22 1.0 23 1.5 24 1.0 25 2.5 26 3.5 27 3.5 28 7.5 29 8.0 30 7.0 31 15.0 32 25.5 33 31.0 34 41.0 35 64.0 36 87.0 37 104.5 38 135.0 39 176.0 40 201.5 41 252.0 42 274.5 43 265.0 44 271.5 45 252.0 46 271.5 47 268.5 48 236.5 49 202.5 50 168.5 51 145.0 52 110.0 53 83.5 54 60.5 55 46.5 56 32.5 57 28.0 58 22.5 59 16.5 60 14.0 61 9.0 62 5.5 63 7.5 64 5.0 65 0.5 66 0.0 67 2.5 68 4.0 69 1.5 70 0.0 71 0.5 72 0.5 73 0.5 74 0.5 75 0.0 76 0.0 77 0.5 78 0.5 79 0.0 80 0.0 81 0.5 82 0.5 83 0.0 84 0.5 85 0.5 86 0.0 87 0.0 88 0.0 89 0.5 90 1.0 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.5 100 2.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150-151 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 61.5 #Duplication Level Percentage of deduplicated Percentage of total 1 64.67479674796748 39.775 2 20.975609756097562 25.8 3 7.1951219512195115 13.275 4 3.9430894308943087 9.700000000000001 5 1.788617886178862 5.5 6 0.8130081300813009 3.0 7 0.3658536585365854 1.575 8 0.08130081300813008 0.4 9 0.08130081300813008 0.44999999999999996 >10 0.08130081300813008 0.525 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source AGAAGAAAGAGTTGATAAAATCCAATCGAATTGAAGTGGATTATTTGATA 11 0.27499999999999997 No Hit GAAGGACCTCATTTAATTAGTTTCAGACCACATGCTCGTAATGTGCCACT 10 0.25 No Hit GGTGCCTGTATGCTTATGAACGATTGCCTTGTTGCAGGGTGACCTTTGTG 9 0.22499999999999998 No Hit TATCTAAAGTATTGTCACTTTACTTAACACTTGAGCTGCATAGAATGTCT 9 0.22499999999999998 No Hit CCTTGGGCTCGGTCTTTGTCGCAGCCATCGTGTCTCTCTTCCTTGGATAC 8 0.2 No Hit GTCTATAGGCCCGTCTGGCAGCTTACACCAAAAGGCTCGGGCTGCTTGGC 8 0.2 No Hit GCAAGCTGCTACCCCCCCCCACCAAACCAAACCAAACCAACTTAATTTCA 7 0.17500000000000002 No Hit AGAAGGGCCATGAAGAGGAGCCACCATTTGAGTTCAAGATAGATGAAGAG 7 0.17500000000000002 No Hit GGCTAAGGAGTTTTGTGGATGAACCTTCTTTTACGGATCTTCATTGGGGA 7 0.17500000000000002 No Hit GTTGAAATGGGTCCGGGAGAATTATATGGTATACAATTACTGCAAAGATG 7 0.17500000000000002 No Hit CTCTTCTATAGCTTCTATTAGCTAGTATTCACTGTAGCTTCTAAATTCAA 7 0.17500000000000002 No Hit GAACAAATGATCAATGTCCAGAACAAGAATTCATCCTACTTTGTTGAGTG 7 0.17500000000000002 No Hit CTCTATTCCAGATAATCAGGACAGCTTCAAGTGGAGGCATGGCCTAAGCC 7 0.17500000000000002 No Hit CAAACACTCAAATTCACAGAAAAGTGTGACATTTACAGTTTTGGTGTGGT 7 0.17500000000000002 No Hit GCTGCCGTTGCTGCCTTGTGTGTGCAATATGAGGCTGATTTCCGGCCAAA 7 0.17500000000000002 No Hit GGAAAGACTAACCCTAATTTATTCTAAACCATCATGCTACTCTCTCCAGG 6 0.15 No Hit TTCTTTCCAAGAAACCAATAAGCACAGGACTGTTTCTGTAGTTACTGGCA 6 0.15 No Hit GGTAGAGCTTGGAAAAGAGGGTACCTGCTCTACGGTCCACCAGGGACTGG 6 0.15 No Hit GCATTTTGCTCTTGGGTCCGTGCATACACAGCCCATCGTGGTGACCTGAA 6 0.15 No Hit GTCCTTATGGACCTTGAACCTGGTACTATGGATAGTGTCAGGTCCGGTCC 6 0.15 No Hit GTTGCTGTTGGGAATGAGGATTGTGGGAAGAAGAATTACCGAGGAAATTA 6 0.15 No Hit AGAAGATTGAGTATTCTATGCAGCTTAATGCTTCTCGGATCAAAGTGCTT 6 0.15 No Hit CTTTTGAGAGCTCAACTTCTTTTGCTTCGATCCCTCTGCAACCAATCCCC 6 0.15 No Hit CTATTCTTGGCTGTGACTATGGTTTCAACTTCTCCTTCCAAGGAAGATCA 6 0.15 No Hit CAAGGTTAAGAGGGCCATCATGGGTAGTGTAAGCAACTATGTGGTGAATA 6 0.15 No Hit GATTATAGCTCCAGGGATTTTAACAGCTCCGGCCCCAACACCGTCAAGTG 6 0.15 No Hit GCCTGATTGATTTGTTACACTCATGGCAAGATGGAAGACTTCCGGTTGAT 6 0.15 No Hit TGAACTTTGGTGCTGAGAATGGCTGCAAGTGTGGATCAAGCTGCACCTGT 6 0.15 No Hit AGCGGAAAACAAGTCTCAGAAAAAGCTCTCTGCTGTTGTTGCATGGGAGA 6 0.15 No Hit CCCTCAACAATCTCAACACAAGAAAAGAGTGGCCCCTGCTGCACCAATAG 6 0.15 No Hit GATGATTGTATCACCCATTGGAGGGATGAGGCAAATCATAATTCAAGCCA 6 0.15 No Hit AACCAGTAGCTAGAGAGATAGAGAGAGGAGGAAAGGCTTGTGGCAAGAGA 6 0.15 No Hit GGGGCAAGGTGAAATATGAACTTGACAAAAAAACTGGACTGATCAAGGTT 6 0.15 No Hit CTAGTTGGGAATTCTGGACTCCCTTTGATGGATTGGGTTCTGGTGCCTTA 6 0.15 No Hit GAGATCGAGGAAGTTCAAGCAAAAACCAAGACAATTTCAGGTGCACTCTA 6 0.15 No Hit GCCAAGATTGGAGAGGGTTGTTTGATTGGACCTGATGTTGCAATAGGACC 5 0.125 No Hit TCCAGATGGCAAGGCTTCACAGAATGTTAGGCTTGTTGATTATTTCAGGC 5 0.125 No Hit CACTCTTTCTTGGTCCGCTTCTGTTAAAAAGTCTGAGGTGTTTGCTGTTG 5 0.125 No Hit CATGAAGTACCTCCGTCACCTACCACGCAGGTTCAAGACTAACTTTAGAG 5 0.125 No Hit ATCAATTGAAGGGAGATTGAAATGGAAGGTGATTTATTTTCAGGCTTAAG 5 0.125 No Hit CTGAGCTTGAGTGCATTCTTCTAAGTAAAAGAAATCCCTTAATTTCATCA 5 0.125 No Hit GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG 5 0.125 No Hit GTTAGGTGTTGCCGTTAGAGCGCCGTCCGAGTTTTTGTGGGCTGACTCGT 5 0.125 No Hit GGAGTCTGACATGTGTGCGAGTCAACGGGCGAGTAAACCCGTAAGGCGCA 5 0.125 No Hit GCAACCAGCTTGCGGAAGCAGATGAGTTTGACGACAAGATGAAGGAGCTT 5 0.125 No Hit TGAACATTTCCACAAACGTCAGCCTTGATGGCGTTGATGTCTCTGCTATC 5 0.125 No Hit AAGAAAATCAATAGCAAAGTATCCAGAGATGGAAGAGCAGAAGCCCAACA 5 0.125 No Hit GCACATTGGAAGGATGGAAGTACACAGCGAAGAATTTGCTGATGTATCAT 5 0.125 No Hit CTTTGTCAAATGAAAAGCTCTTCGTGGTGTTCCAAGGTGTTTCAAATGTA 5 0.125 No Hit GAGAGAGAGAGAGAGAGAGAGAGATACTGTTCTCATGGAAGAAGCAAAAG 5 0.125 No Hit GATACCTACTCAAGCCACCTCTCTAACTCACCTCTCTCTCACTTCTTCCA 5 0.125 No Hit CCTGCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTGTAA 5 0.125 No Hit CTCTCTGGGTTGCTTAAATTTAATTGGCAAAACCATTGCTTTGGCAGAAT 5 0.125 No Hit GTCCCATTAAATGCAGTGCAGCTCCTGTGGGTTAACCTTATCATGGATAC 5 0.125 No Hit GTGGGAATCATTATAGCTAGGTACCTTAAGGTGTTCAAGTCAGCAGGCCC 5 0.125 No Hit CTGGGGAACTTGAGTGGGGGTGCATGATGGGCTCCTTGTCATAGAACCCC 5 0.125 No Hit AGACAAGGTGCTGTGTTTGGTGGGCTGCTAGAATGTCCATTGAAACCTAC 5 0.125 No Hit GATGATGAGAACAAGATCATAACTCTTAATGGTTTGGAAGGAGATGTCAT 5 0.125 No Hit GGGAAGCACACAGGAGGGGCAGATCTAGGAATGGAGATGTGTATTTAAAC 5 0.125 No Hit AAAAGGAATCTGCAGATGGAACTGTCAGAATCTCAGAACAGGAAAATCCA 5 0.125 No Hit ATTGTCACTTTACTTAACACTTGAGCTGCATAGAATGTCTACAGTCAATT 5 0.125 No Hit TGATGAGATTAGTGTTTTAGAAGAACTGGAAATGATTGGTAATGATATTG 5 0.125 No Hit GATGGAAATTTCTTATAACATCATTCAAAGAACAAACCGGCCATGCATTC 5 0.125 No Hit GCAAAGCAATGTGTGGATGAGGATCCCATCCTACGGCCTGACATGAAGCA 5 0.125 No Hit ACTTAGAGCAAGGAGATGTGGTGATGAATTCTTCAGCAGGAGATATTGAT 5 0.125 No Hit GACCATTACCCTTGAGGTTGAGAGTTCTGACACCATTGATAATGTGAAAG 5 0.125 No Hit CTAAGCTTGACAAGAAGAGGAAACAGCATTTTCTTTCAAGCAGAGCAGCA 5 0.125 No Hit CACTACTCTTCCTCTCTATAATTTTCTAGGGTTTAGCAATGTCTGCCGAG 5 0.125 No Hit CGAATGCTTGGTTCTCAGAGGAGCTTGATTCAACAAGGCAGGAGAGGTTG 5 0.125 No Hit TATGCCTCTTAAGATTTGTTACCCTGCTTTGGAATATGAAGAATGGCGCA 5 0.125 No Hit GTTAGCCGAAAGATGGTTATCGGTTCAAGGACGCACGGTGTCCCTGTTTT 5 0.125 No Hit AGAGGAGATCCCAGATGGCAGAATTCAAATGGTTCTACCGGGGAGTGAAA 5 0.125 No Hit CAATTCGAGAGTTCAAGAACCTGGAGTCTATGGGGGTTCCCTTTCCAAAA 5 0.125 No Hit CTTTTTTTAACATCTTGAATAGAGTTTCGCCGTTTTGTTTTTTATTTGGT 5 0.125 No Hit GGGATCACCCCCCTCTATATTGGCTGGGGACTTGATGGGTCCGTGTGGAT 5 0.125 No Hit GTCTCTGGTCTTGGTCCATTTGAATTGGAAGCCTTGCAGGACTGGGAATA 5 0.125 No Hit CTGGACATTGTCTCTGATTTTAGCTAAGAACCTTACTCTAACTGAACATT 5 0.125 No Hit CTTCGAATGAAGCAGGAAATGATGCTTTCCAACAAGTTAAAACTGGAGAT 5 0.125 No Hit CTTGATTTGCTCGGAGATAGCTCCAGAGATGTGGCGCGGCTCTGTTTCGA 5 0.125 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0125 0.0 0.0 0.0 0.0 52-53 0.025 0.0 0.0 0.0 0.0 54-55 0.025 0.0 0.0 0.0 0.0 56-57 0.025 0.0 0.0 0.0 0.0 58-59 0.05 0.0 0.0 0.0 0.0 60-61 0.05 0.0 0.0 0.0 0.0 62-63 0.05 0.0 0.0 0.0 0.0 64-65 0.05 0.0 0.0 0.0 0.0 66-67 0.05 0.0 0.0 0.0 0.0 68-69 0.0625 0.0 0.0 0.0 0.0 70-71 0.075 0.0 0.0 0.0 0.0 72-73 0.075 0.0 0.0 0.0 0.0 74-75 0.1 0.0 0.0 0.0 0.0 76-77 0.175 0.0 0.0 0.0 0.0 78-79 0.21250000000000002 0.0 0.0 0.0 0.0 80-81 0.2875 0.0 0.0 0.0 0.0 82-83 0.3875 0.0 0.0 0.0 0.0 84-85 0.5125 0.0 0.0 0.0 0.0 86-87 0.6375 0.0 0.0 0.0 0.0 88-89 0.7 0.0 0.0 0.0 0.0 90-91 0.9375 0.0 0.0 0.0 0.0 92-93 1.25 0.0 0.0 0.0 0.0 94-95 1.425 0.0 0.0 0.0 0.0 96-97 1.6749999999999998 0.0 0.0 0.0 0.0 98-99 2.0125 0.0 0.0 0.0 0.0 100-101 2.3 0.0 0.0 0.0 0.0 102-103 2.575 0.0 0.0 0.0 0.0 104-105 2.9625000000000004 0.0 0.0 0.0 0.0 106-107 3.3375 0.0 0.0 0.0 0.0 108-109 3.6500000000000004 0.0 0.0 0.0 0.0 110-111 4.025 0.0 0.0 0.0 0.0 112-113 4.525 0.0 0.0 0.0 0.0 114-115 5.125 0.0 0.0 0.0 0.0 116-117 5.5625 0.0 0.0 0.0 0.0 118-119 6.2125 0.0 0.0 0.0 0.0 120-121 6.75 0.0 0.0 0.0 0.0 122-123 7.2875 0.0 0.0 0.0 0.0 124-125 7.95 0.0 0.0 0.0 0.0 126-127 8.5 0.0 0.0 0.0 0.0 128-129 9.1125 0.0 0.0 0.0 0.0 130-131 9.6625 0.0 0.0 0.0 0.0 132-133 10.537500000000001 0.0 0.0 0.0 0.0 134-135 11.075 0.0 0.0 0.0 0.0 136-137 12.024999999999999 0.0 0.0 0.0 0.0 138-139 12.5875 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGCAAAG 10 0.006830828 145.0 1 CATCTGT 10 0.006830828 145.0 6 >>END_MODULE Read 2352576 spots for SRR26075352.sra Written 2352576 spots for SRR26075352.sra Read 2352576 spots for SRR26075352.sra Written 2352576 spots for SRR26075352.sra Read 2352576 spots for SRR26075352.sra Written 2352576 spots for SRR26075352.sra Read 2352576 spots for SRR26075352.sra Written 2352576 spots for SRR26075352.sra Read 2352576 spots for SRR26075352.sra Written 2352576 spots for SRR26075352.sra Read 2352576 spots for SRR26075352.sra Written 2352576 spots for SRR26075352.sra Read 2352576 spots for SRR26075352.sra Written 2352576 spots for SRR26075352.sra Read 2352576 spots for SRR26075352.sra Written 2352576 spots for SRR26075352.sra Read 2352576 spots for SRR26075352.sra Written 2352576 spots for SRR26075352.sra Read 2352576 spots for SRR26075352.sra Written 2352576 spots for SRR26075352.sra Read 2352576 spots for SRR26075352.sra Written 2352576 spots for SRR26075352.sra Read 2352576 spots for SRR26075352.sra Written 2352576 spots for SRR26075352.sra Read 2352576 spots for SRR26075352.sra Written 2352576 spots for SRR26075352.sra Read 2352576 spots for SRR26075352.sra Written 2352576 spots for SRR26075352.sra Read 2352576 spots for SRR26075352.sra Written 2352576 spots for SRR26075352.sra Read 2352576 spots for SRR26075352.sra Written 2352576 spots for SRR26075352.sra Read 2352577 spots for SRR26075352.sra Written 2352577 spots for SRR26075352.sra Read 2352576 spots for SRR26075352.sra Written 2352576 spots for SRR26075352.sra Read 2352576 spots for SRR26075352.sra Written 2352576 spots for SRR26075352.sra Read 2352576 spots for SRR26075352.sra Written 2352576 spots for SRR26075352.sra SRR ids: ['SRR26075352.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_bj4_zwqt SRR26075352.sra spots: 47051521 blocks: [[1, 2352576], [2352577, 4705152], [4705153, 7057728], [7057729, 9410304], [9410305, 11762880], [11762881, 14115456], [14115457, 16468032], [16468033, 18820608], [18820609, 21173184], [21173185, 23525760], [23525761, 25878336], [25878337, 28230912], [28230913, 30583488], [30583489, 32936064], [32936065, 35288640], [35288641, 37641216], [37641217, 39993792], [39993793, 42346368], [42346369, 44698944], [44698945, 47051521]] SRR26075352 file size 17379153 SRR26075352 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o SRR26075352 SRR26075352_1.fastq SRR26075352_2.fastq Input file: SRR26075352_1.fastq Paired file: SRR26075352_2.fastq trimmed: SRR26075352-trimmed-pair1.fastq, SRR26075352-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Tue Feb 11 22:01:57 2025 >> started Tue Feb 11 22:02:56 2025 >> done (59.668s) 47051521 read pairs processed; of these: 148 ( 0.00%) short read pairs filtered out after trimming by size control 36768 ( 0.08%) empty read pairs filtered out after trimming by size control 47014605 (99.92%) read pairs available; of these: 8305060 (17.66%) trimmed read pairs available after processing 38709545 (82.34%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 18 17 0.00% 19 22 0.00% 20 20 0.00% 21 18 0.00% 22 18 0.00% 23 35 0.00% 24 22 0.00% 25 32 0.00% 26 38 0.00% 27 30 0.00% 28 29 0.00% 29 56 0.00% 30 69 0.00% 31 65 0.00% 32 72 0.00% 33 61 0.00% 34 61 0.00% 35 60 0.00% 36 96 0.00% 37 109 0.00% 38 147 0.00% 39 187 0.00% 40 215 0.00% 41 251 0.00% 42 235 0.00% 43 258 0.00% 44 246 0.00% 45 283 0.00% 46 323 0.00% 47 363 0.00% 48 485 0.00% 49 590 0.00% 50 682 0.00% 51 773 0.00% 52 831 0.00% 53 897 0.00% 54 1015 0.00% 55 1153 0.00% 56 1162 0.00% 57 1463 0.00% 58 1658 0.00% 59 1820 0.00% 60 2256 0.00% 61 2754 0.01% 62 3017 0.01% 63 3231 0.01% 64 3783 0.01% 65 4163 0.01% 66 4564 0.01% 67 5261 0.01% 68 5959 0.01% 69 6571 0.01% 70 7445 0.02% 71 8535 0.02% 72 9999 0.02% 73 11362 0.02% 74 12206 0.03% 75 13722 0.03% 76 15263 0.03% 77 16557 0.04% 78 18070 0.04% 79 20056 0.04% 80 21714 0.05% 81 23975 0.05% 82 26235 0.06% 83 28875 0.06% 84 32148 0.07% 85 35281 0.08% 86 37550 0.08% 87 38931 0.08% 88 40873 0.09% 89 42842 0.09% 90 45696 0.10% 91 48566 0.10% 92 50990 0.11% 93 55173 0.12% 94 58039 0.12% 95 61406 0.13% 96 64790 0.14% 97 66313 0.14% 98 68855 0.15% 99 70552 0.15% 100 73362 0.16% 101 75654 0.16% 102 77877 0.17% 103 81978 0.17% 104 84745 0.18% 105 90240 0.19% 106 93464 0.20% 107 95101 0.20% 108 97297 0.21% 109 99535 0.21% 110 99616 0.21% 111 102458 0.22% 112 106049 0.23% 113 107905 0.23% 114 111837 0.24% 115 116927 0.25% 116 118170 0.25% 117 123762 0.26% 118 126813 0.27% 119 126476 0.27% 120 129161 0.27% 121 130168 0.28% 122 131714 0.28% 123 134649 0.29% 124 138206 0.29% 125 141005 0.30% 126 146262 0.31% 127 150348 0.32% 128 152520 0.32% 129 154526 0.33% 130 156469 0.33% 131 158028 0.34% 132 157692 0.34% 133 160128 0.34% 134 162449 0.35% 135 167374 0.36% 136 170752 0.36% 137 174393 0.37% 138 178456 0.38% 139 180769 0.38% 140 182541 0.39% 141 183376 0.39% 142 186150 0.40% 143 183688 0.39% 144 188959 0.40% 145 191222 0.41% 146 192613 0.41% 147 196886 0.42% 148 199933 0.43% 149 201343 0.43% 150 204469 0.43% 151 38709545 82.34% 47014605 reads passed initial QC criterion=sequence-density sequence-density=0.53 sequence-density-rank=1 fanout-score=2.56 fanout-score-rank=29 prefix-density=0.57 prefix-fanout=2.4 sequence=GGAATTGAATTAATGAACTTGGTGGCAGCAAGGAAGGCGTTGTAGGAGGCTAAATAGCTAACTCC criterion=fanout-score sequence-density=0.01 sequence-density-rank=34 fanout-score=28.29 fanout-score-rank=1 prefix-density=0.07 prefix-fanout=2.2 sequence=AGCTTCTACTTTTATTTAATAGTTTTATAGATTACACAAAGGAAATACAACACAAGATCTCCCCACAAATCACACACATTGATGCAGTACTGAACTCGTTGCACGAAAGCGCTTAGATATATATTATACAAGTACTAGCATGATCACAAACATGTGATGCTTATTGGTCGAGATCGATGACCCCTTCTATTACTCCGTGCTAAGGGCTTCGTCGATGTCTTTAGTCATATGAACCATAAGATCAACATAAATCTCTGGAACCGGGACTTCAGGATGGAGTTTTTCGTATTCAATGGTCAGTTTTGCCAAGCAGCCCGAGCCTTTTGGTGTAAGCTGCCAGACGGGCCTATAGACCTTGTAAATTTTCATGACATCTCCTTCCAAACCATTAAGAGTTATGATCTTGTTCTCATCATCGAAGGAAACCTCCTCTTTAAAGACCCCGGCTTTCCCTCCGATTGTGTACTGCCAAATCCTGATAGAGCCCGCAGTCTCCCAGTCACCTGCATGT criterion=sequence-density sequence-density=0.48 sequence-density-rank=1 fanout-score=4.25 fanout-score-rank=18 prefix-density=0.58 prefix-fanout=3.5 sequence=AGAAAACAATGG criterion=fanout-score sequence-density=0.16 sequence-density-rank=23 fanout-score=25.02 fanout-score-rank=1 prefix-density=0.50 prefix-fanout=8.3 sequence=TGATGAGAAGATG SRR26075352 testing PE reads STAR mapping to Ensembl genome Started job on | Feb 11 22:04:40 Started mapping on | Feb 11 22:04:40 Finished on | Feb 11 22:11:03 Mapping speed, Million of reads per hour | 441.91 Number of input reads | 47014605 Average input read length | 292 UNIQUE READS: Uniquely mapped reads number | 43637763 Uniquely mapped reads % | 92.82% Average mapped length | 290.74 Number of splices: Total | 34878401 Number of splices: Annotated (sjdb) | 33962668 Number of splices: GT/AG | 34347734 Number of splices: GC/AG | 377781 Number of splices: AT/AC | 31496 Number of splices: Non-canonical | 121390 Mismatch rate per base, % | 0.42% Deletion rate per base | 0.03% Deletion average length | 2.88 Insertion rate per base | 0.02% Insertion average length | 2.66 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 1320864 % of reads mapped to multiple loci | 2.81% Number of reads mapped to too many loci | 547806 % of reads mapped to too many loci | 1.17% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 2.93% % of reads unmapped: other | 0.28% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 2055978 2055978 2055978 N_multimapping 1320864 1320864 1320864 N_noFeature 1249574 43126121 1582945 N_ambiguous 458641 3526 278057 UnstrandedReadsAssigned:41929548 PositiveStrandReadsAssigned:508116 NegativeStrandReadsAssigned:41776761 Dataset is classified negative stranded MeadianReadLen=151 20thPercentileLength=151 echo kmer=147 SRR26075352 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,400 [index] number of k-mers: 62,057,036 [index] number of equivalence classes: 130,681 [quant] running in paired-end mode [quant] will process pair 1: SRR26075352-trimmed-pair1.fastq SRR26075352-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 47,014,605 reads, 42,569,913 reads pseudoaligned [quant] estimated average fragment length: 210.377 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,167 rounds 52401 SRR26075352.ke.tsv 34699 SRR26075352.se.tsv 87100 total ==> SRR26075352.ke.tsv <== target_id length eff_length est_counts tpm Potri.005G200100.1.v4.1 2018 1808.62 7120 79.7967 Potri.005G024800.1.v4.1 1035 825.623 19048 467.649 Potri.004G059700.1.v4.1 961 751.629 147 3.9643 Potri.007G009000.2.v4.1 1416 1206.62 0 0 Potri.003G141000.2.v4.1 2943 2733.62 1866.9 13.8431 Potri.016G087400.1.v4.1 270 93.9402 3287.61 709.386 Potri.015G069301.1.v4.1 564 356.758 0 0 Potri.010G195200.1.v4.1 1773 1563.62 20 0.259269 Potri.012G127500.1.v4.1 977 767.623 11360 299.974 ==> SRR26075352.se.tsv <== Potri.001G166300.v4.1 0 Potri.001G448400.v4.1 201 Potri.001G233950.v4.1 0 Potri.001G122700.v4.1 289 Potri.001G212900.v4.1 5 Potri.001G182400.v4.1 0 Potri.001G256600.v4.1 0 Potri.001G040500.v4.1 3 Potri.001G416900.v4.1 0 Potri.001G452600.v4.1 771 SRR26075352 completed mapping pipeline successfully