Starting /dee2/code/volunteer_pipeline.sh SRR26075353 current disk space = 3048989003776 free memory = 1531032188 SRR26075353 SRAfilesize 2652f7699ab8a03f460cdd47efff7f05 SRR26075353.sra SRR26075353.sra file validated SRR26075353 is paired end SRR26075353 is conventional basespace SRR26075353 read1 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR26075353_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.659 37.0 37.0 37.0 37.0 37.0 2 36.5365 37.0 37.0 37.0 37.0 37.0 3 36.572 37.0 37.0 37.0 37.0 37.0 4 36.706 37.0 37.0 37.0 37.0 37.0 5 36.756 37.0 37.0 37.0 37.0 37.0 6 36.6565 37.0 37.0 37.0 37.0 37.0 7 36.6475 37.0 37.0 37.0 37.0 37.0 8 36.625 37.0 37.0 37.0 37.0 37.0 9 36.6245 37.0 37.0 37.0 37.0 37.0 10-14 36.6456 37.0 37.0 37.0 37.0 37.0 15-19 36.687200000000004 37.0 37.0 37.0 37.0 37.0 20-24 36.5553 37.0 37.0 37.0 37.0 37.0 25-29 36.416700000000006 37.0 37.0 37.0 37.0 37.0 30-34 36.3997 37.0 37.0 37.0 37.0 37.0 35-39 36.3775 37.0 37.0 37.0 37.0 37.0 40-44 36.294200000000004 37.0 37.0 37.0 37.0 37.0 45-49 36.1332 37.0 37.0 37.0 37.0 37.0 50-54 36.2106 37.0 37.0 37.0 37.0 37.0 55-59 35.985699999999994 37.0 37.0 37.0 37.0 37.0 60-64 35.908699999999996 37.0 37.0 37.0 37.0 37.0 65-69 35.8809 37.0 37.0 37.0 37.0 37.0 70-74 35.9439 37.0 37.0 37.0 37.0 37.0 75-79 35.995099999999994 37.0 37.0 37.0 37.0 37.0 80-84 35.9794 37.0 37.0 37.0 37.0 37.0 85-89 35.8918 37.0 37.0 37.0 37.0 37.0 90-94 35.9003 37.0 37.0 37.0 37.0 37.0 95-99 35.8851 37.0 37.0 37.0 37.0 37.0 100-104 35.7845 37.0 37.0 37.0 37.0 37.0 105-109 35.74499999999999 37.0 37.0 37.0 37.0 37.0 110-114 35.6158 37.0 37.0 37.0 37.0 37.0 115-119 35.5607 37.0 37.0 37.0 37.0 37.0 120-124 35.5609 37.0 37.0 37.0 37.0 37.0 125-129 35.4747 37.0 37.0 37.0 37.0 37.0 130-134 35.2949 37.0 37.0 37.0 29.8 37.0 135-139 35.2924 37.0 37.0 37.0 34.6 37.0 140-144 35.0513 37.0 37.0 37.0 25.0 37.0 145-149 35.0513 37.0 37.0 37.0 25.0 37.0 150-151 34.96225 37.0 37.0 37.0 25.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.0 1101 2 0.0 1101 3 0.0 1101 4 0.0 1101 5 0.0 1101 6 0.0 1101 7 0.0 1101 8 0.0 1101 9 0.0 1101 10-14 0.0 1101 15-19 0.0 1101 20-24 0.0 1101 25-29 0.0 1101 30-34 0.0 1101 35-39 0.0 1101 40-44 0.0 1101 45-49 0.0 1101 50-54 0.0 1101 55-59 0.0 1101 60-64 0.0 1101 65-69 0.0 1101 70-74 0.0 1101 75-79 0.0 1101 80-84 0.0 1101 85-89 0.0 1101 90-94 0.0 1101 95-99 0.0 1101 100-104 0.0 1101 105-109 0.0 1101 110-114 0.0 1101 115-119 0.0 1101 120-124 0.0 1101 125-129 0.0 1101 130-134 0.0 1101 135-139 0.0 1101 140-144 0.0 1101 145-149 0.0 1101 150-151 0.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 20 2.0 21 3.0 22 2.0 23 7.0 24 7.0 25 13.0 26 16.0 27 18.0 28 19.0 29 17.0 30 21.0 31 41.0 32 51.0 33 122.0 34 181.0 35 446.0 36 2826.0 37 208.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.518277416124185 13.044566850275412 7.361041562343515 34.07611417125688 2 19.7 15.25 33.175 31.874999999999996 3 16.225 18.0 29.45 36.325 4 20.8 24.25 24.325 30.625000000000004 5 26.450000000000003 27.250000000000004 23.974999999999998 22.325 6 24.25 32.2 21.275 22.275 7 17.825 28.625 37.85 15.7 8 16.725 26.55 32.125 24.6 9 19.925 24.175 33.85 22.05 10-14 19.97 30.195 26.69 23.145 15-19 21.305 28.475 26.924999999999997 23.294999999999998 20-24 20.515 26.93 28.27 24.285 25-29 20.549999999999997 27.61 27.18 24.66 30-34 19.78 27.435 28.285 24.5 35-39 22.125 26.884999999999998 26.619999999999997 24.37 40-44 21.48 27.22 27.189999999999998 24.11 45-49 21.25 27.495000000000005 26.950000000000003 24.305 50-54 21.48 27.36 26.91 24.25 55-59 21.565 27.755000000000003 27.42 23.26 60-64 21.36 26.305 27.93 24.404999999999998 65-69 21.325 27.025 27.905 23.745 70-74 22.445 25.945 27.765 23.845 75-79 21.97 26.97 27.36 23.7 80-84 22.66 26.96 26.889999999999997 23.49 85-89 22.865 26.790000000000003 26.665 23.68 90-94 21.765 26.705000000000002 27.595 23.935000000000002 95-99 22.415 26.150000000000002 26.640000000000004 24.795 100-104 22.34 27.775 25.814999999999998 24.07 105-109 22.595000000000002 26.784999999999997 26.810000000000002 23.810000000000002 110-114 22.365 27.150000000000002 26.555 23.93 115-119 22.935 27.115000000000002 25.945 24.005000000000003 120-124 22.925 26.529999999999998 26.61 23.935000000000002 125-129 22.78 27.205000000000002 25.715 24.3 130-134 21.990000000000002 27.415 26.465 24.13 135-139 22.55 25.995 26.075 25.380000000000003 140-144 23.27 27.189999999999998 25.215 24.325 145-149 23.735 26.314999999999998 25.929999999999996 24.02 150-151 23.9375 25.8625 26.1625 24.0375 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.5 7 1.5 8 1.0 9 0.0 10 0.0 11 0.5 12 0.5 13 0.0 14 1.0 15 1.0 16 0.0 17 0.0 18 0.0 19 0.5 20 1.0 21 1.5 22 1.5 23 3.0 24 3.5 25 1.5 26 1.0 27 4.0 28 9.5 29 10.0 30 8.0 31 13.0 32 22.0 33 37.5 34 45.0 35 48.5 36 68.0 37 100.0 38 138.0 39 136.0 40 139.5 41 168.0 42 187.5 43 215.5 44 238.0 45 256.0 46 249.0 47 245.5 48 228.0 49 206.5 50 213.0 51 180.0 52 136.0 53 111.5 54 98.0 55 87.5 56 65.5 57 45.0 58 36.5 59 30.0 60 26.0 61 27.0 62 17.5 63 10.5 64 14.5 65 18.5 66 19.0 67 19.5 68 22.5 69 15.5 70 5.5 71 2.0 72 1.5 73 3.0 74 2.0 75 0.0 76 0.0 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.15 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150-151 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 61.375 #Duplication Level Percentage of deduplicated Percentage of total 1 62.64765784114053 38.45 2 23.380855397148675 28.7 3 8.268839103869654 15.225 4 2.8513238289205702 7.000000000000001 5 1.7515274949083504 5.375 6 0.6517311608961304 2.4 7 0.1629327902240326 0.7000000000000001 8 0.0814663951120163 0.4 9 0.0814663951120163 0.44999999999999996 >10 0.12219959266802445 1.3 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTGGACGTATCTCGTAT 31 0.775 TruSeq Adapter, Index 3 (97% over 37bp) GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTGGACGTATCGCGTAT 11 0.27499999999999997 TruSeq Adapter, Index 3 (97% over 37bp) CTTTGAAAATGAAGAAGAAATATCAGTGTTCCTATAAAGGCCATCGGAAA 10 0.25 No Hit GGAAGGGCAAAACGAGATGCAATTATGGAGCAATGAGCCGCGTATTTACG 9 0.22499999999999998 No Hit ATCTTTTTTCCATCATTTGAAATCACATAATCACGTGAGCTTGAAACCAA 9 0.22499999999999998 No Hit TCCCCCCAGAACCCAAAAACTTTGATTTCTCATAAGGTGCTGGCGGAGTC 8 0.2 No Hit GCCTGAATGGTGACAGTTTTCTTGTTTGCCAAACCAGAGTGCTTGTAAGA 8 0.2 No Hit GCCTCGCTGAGTCCATTGAAGAAGCTTCTTTCCACTGTTTCTAGAACCTT 7 0.17500000000000002 No Hit GCTGTTTTCTTCCTTCCGAAGCACTGCACGGACTCCGTCGGCGCCGCCAT 7 0.17500000000000002 No Hit CCGAGCTGCTGGAGGAGGACTGGACGCTGTTGCAGCACTGAGGGGACGAC 7 0.17500000000000002 No Hit ATCTTCCTTCCCACAGCTGGATAGCTAATATTTGTTCTTCTTCCACAAGA 7 0.17500000000000002 No Hit CCTACTGCTGGAACTAAATCCTACAAGATCCGAATACATCATGGCCGAAG 6 0.15 No Hit CTTCAGATTTGATGGAAGATTGGTGAACAAATAGATCATCACCGCCATCA 6 0.15 No Hit GCAAACTTTCAGGATGGTGGGAGAAGAAGTCGAGAATCCTCCAGTTCTCC 6 0.15 No Hit GCTGACATCCTAGCTGCTGTACTAATGCTCTTTCAATTTGCTTCCCGTTT 6 0.15 No Hit CTTCAGTTCTCAAAATAAATCACACGACATTGTCAAATTACAGTCATAGA 6 0.15 No Hit GGCTTGTCTGAGGGCCTCTTGGGCTCCTGGATCTGGTCGAGGGCATCCAG 6 0.15 No Hit CCTTGGATATGCTTGGAAGCATGTTTGGGAACATGGAAGGACTGGCTCCT 6 0.15 No Hit CTGGCTTTGGCATTCTGAACTGCACGGTGAAGTTCACTTAAGAAGTCCAT 6 0.15 No Hit ATCCCAGTTGCTAACAATACCATGCTCAATAGGGTATTTCAAGGTCAAGA 6 0.15 No Hit GGCTTGAAAAAAAAGAAATCTTTAAAGAGTAGAGGTAGACGGCTGCACAA 6 0.15 No Hit TCCATATCTTCCTCCCAAAGTCATACATCTTTTTGTCAACAAAATCTGCC 6 0.15 No Hit GCCGCGTCTGGTGGTGGACGGCAGAGGGAGCCTGGTGGACTGCGAGGTCA 6 0.15 No Hit CCCTGTTATATTCTTCACACTGGTCCAGCTACACGCGCCACTGTCATCTC 6 0.15 No Hit GTCTACTTTGATGCGTTCAGCAATTTGTGGACCTCGGACGGGGTAGGGAG 6 0.15 No Hit GCCAGCTATCAGTAGCCCCAAAGATGCAACTAGCAACAGCTGCACCCCGA 6 0.15 No Hit GGTCACTGGTGTCGTCGGTGTCCCTGGAGTTCCTGGCATAGTCATGGACC 6 0.15 No Hit GCCCAATCAAGCCACTGGTTCTCACAACAATAATTCCAACACAAATACAA 5 0.125 No Hit GCAGCATTCCCCAACTCCTTTTCTTTCACTGCTTGCGCCTTCCTCTCCTT 5 0.125 No Hit GTCAGATAACATTATATGACCATCTTCACGTAGTAATATGTTCTCCGGCT 5 0.125 No Hit GTCTATATATCACACTTGAGCTCGCAAAATCCAGACATACAAGTCAATAC 5 0.125 No Hit GCAAGCTTCAACAAGGTTCACAGTGCCTAAATTATCAACCTTCCATGGAG 5 0.125 No Hit GTCCGTTTTTCACAAGATTTGCGGCAATTTGATCCTCATCAAGGGAAACA 5 0.125 No Hit GCATCTTCTGAAGCCACCTGCAGGTCATCACTAGACAGTATTGCTTCAAT 5 0.125 No Hit CTGCAAACATCATGACAGACTCGGGCTCGGGAATTAAGCAAGAGAAAGGC 5 0.125 No Hit CTGCACCCCTGTACTACTTGTCCTGTTACCTATGGCCGGAATAACTGATG 5 0.125 No Hit CTCAGTTGGATTCTCAGCAACTCCATGGAATTTCTTGAATCCAGTCACAT 5 0.125 No Hit GGGCCATGGAAATGAAGAGTGTGAATCAGTTCCTAAGGTGGTGGAAGCAT 5 0.125 No Hit ATTCCCTTCAAGTTCAAGCAGCAGTTTCAGCAGGGGCCTCGGCGATTGGC 5 0.125 No Hit CTCGGGAAGTTTTTTACGGAATGCAACATCCAAACGCGCATCAAGGGAAT 5 0.125 No Hit CTCAAGTATGTGGAAGAGCCTTTCTTCAACCCAGGCATTGAGACTTGTTG 5 0.125 No Hit CGATCCACAATCCACCCTAAATACGGGATGGATTCATCTGCTGAGATGCT 5 0.125 No Hit CTGTCCATCGAAGTCTCTGTATCCGACCGCATCCCACCCAAAACCAATAG 5 0.125 No Hit ACCAAAGTTGCTATCACCATACCAGTTGTGGTCCTTCCACGCCCCATTTG 5 0.125 No Hit CTTCACACAATTCAATCAAACAAATTCTACAAACAGCCTCTTCTTCGGGT 5 0.125 No Hit GTCCTCAAGGAGGGTATGATTTTGCAAAAACCCATCGGGAAGCTCCCTCA 5 0.125 No Hit GGCCGTAAATGCGAATCTGCCCAACAATCCGATATTAGCTTGCCCAGTGG 5 0.125 No Hit AGCAAGGTTGTGTATTTTCTTTTCAGTGATGGTAGCCACCACAGCAGCGA 5 0.125 No Hit GACCGCACAGGTTCCACGCGTGACACCAGCAGCGCTGGGCGCTTTGGTTG 5 0.125 No Hit GGTGTTAGGATCAAAGATAGCAAGATTGACATAAGAATTGGAATAGGCAG 5 0.125 No Hit CTTCGCTAGACATTACGGGTATATATATTTATGTATAACTGGACACCATA 5 0.125 No Hit CCTCCCTCACATGCGACTATGTTCACTATGTCCCTTGCCAGGCACTGTTT 5 0.125 No Hit CATGCATTTGGATTCTCTTCCATGCTCTGGACATAGTAATATGTGGTCAT 5 0.125 No Hit CAGTTGAAGGACTTATCATCTGCACCCTTATGTGTACGTACAGTACGGAT 5 0.125 No Hit CCTACAGCGAAAAAAGAACGATTTTCTGGCATGTCCTTGCCTCGCCTCCA 5 0.125 No Hit ACTAGCTACTTTATTGAAACTTGTTGAAGACAAGAGACAACCCTTATAAA 5 0.125 No Hit GATGGATTCCATCCTTTCAAATGGGTCTGCACCACCTTTGCTCGGAGAAA 5 0.125 No Hit GAACGCGAAAAGAGAGAACTGTATTCTATATTCTCCAAAACAAGAGACCA 5 0.125 No Hit GGGAGCGTTGCCACCGATGCGAAAGAGTTCGACAAGATCATGGAATTGAC 5 0.125 No Hit GCTCCTTCAGGTGAGCTATAACTTCAGCAAGCAACGATGCCTTGTCCATC 5 0.125 No Hit GGAGATGGTTATAGATGTGTGTGGGTGGCGAGAGAAGGAAGAGAAATGTG 5 0.125 No Hit CTCCCAGTTCAGGTCCATAGCACGGTAGTAGTTGAGAGGACCAGTGAAGC 5 0.125 No Hit CAGCAATTCCAAAGAGACCAATAAGAGCGAAGTTACGACGAGTCCAGCGA 5 0.125 No Hit GGAACTATGGGTTTCATTAATATATTGTCGATCTCATTGTGTACCCTCCA 5 0.125 No Hit CAATATTCTTAGTAGCAAGGTTGCTACCAGGGTGGAACACGCTCCTCCAA 5 0.125 No Hit GTGAATTGACAAGTTCTTTTCCAAAAATTACAGCAGAAGAAACATCTTCA 5 0.125 No Hit AGAGATCTAAAGGGAGGAGGGGGGGAAGAAGGATTCTACGAACACTGGAC 5 0.125 No Hit GAATGAATCAGTTAGATAAATATCGCACAAAAAATAGAGAGAGATTTATA 5 0.125 No Hit CCACGAATTTAAAATGCTGCTCTTGAATCCCTTTTGGCTTTTACGCATCA 5 0.125 No Hit GTCCCATGCTGGTATTGAAGTGTTGAAACATTAGTTCTTGGCAGATTTTC 5 0.125 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0125 0.0 0.0 0.0 0.0 34-35 0.025 0.0 0.0 0.0 0.0 36-37 0.025 0.0 0.0 0.0 0.0 38-39 0.025 0.0 0.0 0.0 0.0 40-41 0.025 0.0 0.0 0.0 0.0 42-43 0.025 0.0 0.0 0.0 0.0 44-45 0.025 0.0 0.0 0.0 0.0 46-47 0.025 0.0 0.0 0.0 0.0 48-49 0.025 0.0 0.0 0.0 0.0 50-51 0.025 0.0 0.0 0.0 0.0 52-53 0.025 0.0 0.0 0.0 0.0 54-55 0.025 0.0 0.0 0.0 0.0 56-57 0.025 0.0 0.0 0.0 0.0 58-59 0.025 0.0 0.0 0.0 0.0 60-61 0.025 0.0 0.0 0.0 0.0 62-63 0.025 0.0 0.0 0.0 0.0 64-65 0.025 0.0 0.0 0.0 0.0 66-67 0.025 0.0 0.0 0.0 0.0 68-69 0.025 0.0 0.0 0.0 0.0 70-71 0.025 0.0 0.0 0.0 0.0 72-73 0.025 0.0 0.0 0.0 0.0 74-75 0.075 0.0 0.0 0.0 0.0 76-77 0.15 0.0 0.0 0.0 0.0 78-79 0.15 0.0 0.0 0.0 0.0 80-81 0.16249999999999998 0.0 0.0 0.0 0.0 82-83 0.2 0.0 0.0 0.0 0.0 84-85 0.3375 0.0 0.0 0.0 0.0 86-87 0.4125 0.0 0.0 0.0 0.0 88-89 0.6375 0.0 0.0 0.0 0.0 90-91 0.8999999999999999 0.0 0.0 0.0 0.0 92-93 1.025 0.0 0.0 0.0 0.0 94-95 1.0875 0.0 0.0 0.0 0.0 96-97 1.1875 0.0 0.0 0.0 0.0 98-99 1.4 0.0 0.0 0.0 0.0 100-101 1.75 0.0 0.0 0.0 0.0 102-103 2.0 0.0 0.0 0.0 0.0 104-105 2.425 0.0 0.0 0.0 0.0 106-107 3.0625 0.0 0.0 0.0 0.0 108-109 3.5625 0.0 0.0 0.0 0.0 110-111 4.025 0.0 0.0 0.0 0.0 112-113 4.5 0.0 0.0 0.0 0.0 114-115 5.0 0.0 0.0 0.0 0.0 116-117 5.75 0.0 0.0 0.0 0.0 118-119 6.4625 0.0 0.0 0.0 0.0 120-121 7.0 0.0 0.0 0.0 0.0 122-123 7.862500000000001 0.0 0.0 0.0 0.0 124-125 8.4625 0.0 0.0 0.0 0.0 126-127 8.962499999999999 0.0 0.0 0.0 0.0 128-129 9.774999999999999 0.0 0.0 0.0 0.0 130-131 10.55 0.0 0.0 0.0 0.0 132-133 11.4875 0.0 0.0 0.0 0.0 134-135 12.2375 0.0 0.0 0.0 0.0 136-137 12.850000000000001 0.0 0.0 0.0 0.0 138-139 13.575 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content pass >>END_MODULE SRR26075353 read2 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR26075353_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.1195 37.0 37.0 37.0 37.0 37.0 2 36.303 37.0 37.0 37.0 37.0 37.0 3 36.179 37.0 37.0 37.0 37.0 37.0 4 36.303 37.0 37.0 37.0 37.0 37.0 5 36.2085 37.0 37.0 37.0 37.0 37.0 6 36.1205 37.0 37.0 37.0 37.0 37.0 7 36.1735 37.0 37.0 37.0 37.0 37.0 8 36.181 37.0 37.0 37.0 37.0 37.0 9 36.2055 37.0 37.0 37.0 37.0 37.0 10-14 36.126000000000005 37.0 37.0 37.0 37.0 37.0 15-19 36.050200000000004 37.0 37.0 37.0 37.0 37.0 20-24 35.90839999999999 37.0 37.0 37.0 37.0 37.0 25-29 35.8318 37.0 37.0 37.0 37.0 37.0 30-34 35.6673 37.0 37.0 37.0 37.0 37.0 35-39 35.6356 37.0 37.0 37.0 37.0 37.0 40-44 35.546200000000006 37.0 37.0 37.0 37.0 37.0 45-49 35.396300000000004 37.0 37.0 37.0 37.0 37.0 50-54 35.2963 37.0 37.0 37.0 37.0 37.0 55-59 35.2745 37.0 37.0 37.0 37.0 37.0 60-64 35.4276 37.0 37.0 37.0 37.0 37.0 65-69 35.2547 37.0 37.0 37.0 37.0 37.0 70-74 35.15050000000001 37.0 37.0 37.0 37.0 37.0 75-79 35.1123 37.0 37.0 37.0 37.0 37.0 80-84 35.189800000000005 37.0 37.0 37.0 37.0 37.0 85-89 35.2155 37.0 37.0 37.0 37.0 37.0 90-94 35.153099999999995 37.0 37.0 37.0 34.6 37.0 95-99 35.2697 37.0 37.0 37.0 37.0 37.0 100-104 35.0868 37.0 37.0 37.0 27.4 37.0 105-109 35.080499999999994 37.0 37.0 37.0 29.8 37.0 110-114 35.0524 37.0 37.0 37.0 27.4 37.0 115-119 35.1349 37.0 37.0 37.0 29.8 37.0 120-124 34.955799999999996 37.0 37.0 37.0 27.4 37.0 125-129 34.9794 37.0 37.0 37.0 25.0 37.0 130-134 34.915200000000006 37.0 37.0 37.0 27.4 37.0 135-139 34.7911 37.0 37.0 37.0 25.0 37.0 140-144 34.80675 37.0 37.0 37.0 25.0 37.0 145-149 34.72205 37.0 37.0 37.0 25.0 37.0 150-151 34.473625 37.0 37.0 37.0 25.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.0 1101 2 0.0 1101 3 0.0 1101 4 0.0 1101 5 0.0 1101 6 0.0 1101 7 0.0 1101 8 0.0 1101 9 0.0 1101 10-14 0.0 1101 15-19 0.0 1101 20-24 0.0 1101 25-29 0.0 1101 30-34 0.0 1101 35-39 0.0 1101 40-44 0.0 1101 45-49 0.0 1101 50-54 0.0 1101 55-59 0.0 1101 60-64 0.0 1101 65-69 0.0 1101 70-74 0.0 1101 75-79 0.0 1101 80-84 0.0 1101 85-89 0.0 1101 90-94 0.0 1101 95-99 0.0 1101 100-104 0.0 1101 105-109 0.0 1101 110-114 0.0 1101 115-119 0.0 1101 120-124 0.0 1101 125-129 0.0 1101 130-134 0.0 1101 135-139 0.0 1101 140-144 0.0 1101 145-149 0.0 1101 150-151 0.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 12 1.0 13 3.0 14 10.0 15 5.0 16 14.0 17 16.0 18 7.0 19 10.0 20 11.0 21 12.0 22 17.0 23 16.0 24 16.0 25 28.0 26 22.0 27 15.0 28 17.0 29 22.0 30 23.0 31 24.0 32 57.0 33 92.0 34 172.0 35 637.0 36 2552.0 37 201.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 47.55 22.025 9.275 21.15 2 29.9 24.95 26.825 18.325 3 24.9 28.075 28.7 18.325 4 29.45 31.324999999999996 19.925 19.3 5 26.674999999999997 34.925 20.0 18.4 6 24.925 37.4 20.95 16.725 7 25.074999999999996 19.7 34.025 21.2 8 25.55 25.275 23.575 25.6 9 26.075 24.825 26.424999999999997 22.675 10-14 26.99 27.950000000000003 24.335 20.724999999999998 15-19 26.340000000000003 28.32 24.55 20.79 20-24 26.83 27.339999999999996 25.85 19.98 25-29 26.465 27.055 26.009999999999998 20.47 30-34 26.650000000000002 27.310000000000002 25.53 20.51 35-39 26.275 26.695 26.284999999999997 20.745 40-44 26.0 27.755000000000003 25.495 20.75 45-49 25.845000000000002 27.765 25.95 20.44 50-54 24.95 27.555000000000003 27.189999999999998 20.305 55-59 25.919999999999998 27.91 25.259999999999998 20.91 60-64 26.484999999999996 27.765 24.4 21.349999999999998 65-69 26.179999999999996 27.224999999999998 25.85 20.745 70-74 26.650000000000002 27.6 25.405 20.345 75-79 24.26 28.794999999999998 25.605 21.34 80-84 25.27 27.815 25.825 21.09 85-89 26.615 26.805 25.509999999999998 21.07 90-94 25.230000000000004 27.875 25.535000000000004 21.36 95-99 26.085 27.425 25.775 20.715 100-104 25.215 28.7 24.88 21.205 105-109 25.814999999999998 27.97 25.64 20.575 110-114 26.865 29.054999999999996 24.66 19.42 115-119 26.625 28.134999999999998 25.014999999999997 20.225 120-124 26.88 28.88 24.785 19.455 125-129 27.544999999999998 28.515 24.875 19.064999999999998 130-134 27.73 27.935 24.905 19.43 135-139 28.08 27.08 25.990000000000002 18.85 140-144 28.196409820491024 27.646382319115958 25.06625331266563 19.09095454772739 145-149 28.339250887633145 27.70915637345602 24.908736310446567 19.04285642846427 150-151 28.978622327790976 27.028378547318415 25.19064883110389 18.802350293786724 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 1.0 6 1.5 7 1.0 8 2.0 9 2.5 10 1.5 11 2.0 12 3.0 13 3.0 14 1.5 15 0.0 16 0.5 17 2.0 18 2.0 19 0.5 20 0.0 21 0.5 22 1.0 23 3.0 24 2.5 25 0.0 26 2.0 27 3.5 28 4.0 29 5.0 30 6.5 31 10.5 32 14.5 33 19.0 34 18.0 35 18.0 36 38.5 37 67.0 38 92.0 39 123.0 40 148.5 41 189.5 42 228.0 43 231.5 44 256.0 45 296.5 46 290.5 47 260.5 48 230.0 49 197.5 50 180.0 51 179.5 52 153.5 53 108.0 54 83.5 55 69.5 56 65.5 57 56.5 58 43.5 59 36.5 60 29.0 61 18.5 62 15.5 63 19.0 64 12.5 65 8.5 66 7.5 67 3.5 68 3.5 69 3.0 70 2.0 71 3.5 72 4.0 73 2.0 74 2.5 75 3.5 76 2.5 77 2.0 78 2.5 79 2.0 80 2.0 81 5.0 82 6.0 83 5.0 84 7.5 85 6.5 86 4.0 87 5.5 88 4.0 89 1.5 90 4.5 91 4.5 92 1.5 93 1.0 94 1.0 95 1.0 96 1.0 97 0.5 98 1.0 99 2.5 100 16.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.005 145-149 0.015 150-151 0.0125 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 62.3 #Duplication Level Percentage of deduplicated Percentage of total 1 64.64686998394865 40.275 2 21.990369181380416 27.400000000000002 3 7.825040128410915 14.625 4 2.8089887640449436 7.000000000000001 5 1.7656500802568218 5.5 6 0.521669341894061 1.95 7 0.16051364365971107 0.7000000000000001 8 0.04012841091492777 0.2 9 0.08025682182985554 0.44999999999999996 >10 0.16051364365971107 1.9 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG 45 1.125 No Hit CCCCACGTAGTTCAAGCTCTCTCTCAAACAAATATGTTCTCTAAACTCTC 11 0.27499999999999997 No Hit GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGT 10 0.25 No Hit ATACAAAGGGCATTCCTGCTTCTGGTTACAAGGATCTGAGATCTTGTTTT 10 0.25 No Hit GTAAGGTTTCCTGCGATAATGGAGTCAGCACTGTCAAGGAACATGGCGTG 9 0.22499999999999998 No Hit ATGCAACCTACTACATACTTTGCAGTGTGTCTGCTGCTGGGAGGATCGTG 9 0.22499999999999998 No Hit CGAACAACTGCGAAAGCATTTGCCAAGGATGTTTTCATTAATCAAGAACG 8 0.2 No Hit TGATCAACGGAGTGGAAGCAAGCAAGAAGAGAGGGGACAAATGCGATCAA 7 0.17500000000000002 No Hit AGGGGATTAAAGGGAAATATGGTGGGATTCATCAAATTAGCTGCGGTGAG 7 0.17500000000000002 No Hit TGTGAAGGAGAAAGCATAACGTGTTTCTTAGTCCACCTTTACATGAATTT 7 0.17500000000000002 No Hit CTTGTGAGTTTTTAAGCGCTCGGCTGCAACCAAACCCCGAGAAGAAAACC 7 0.17500000000000002 No Hit CCTGAAACCCTGAGGGATCCTCGTGGTTTTGCAGTGAAGTTTTACACCAG 6 0.15 No Hit CGATAATGCATAGCTACAATGATACAGCGATAGATTTATCTGAAAGACCA 6 0.15 No Hit TGGTGATCAAGTAACCCTGTTGGATTTCTGGTCAAGTCCATTTGGTATGA 6 0.15 No Hit TTTGAAACTTGCAGGTCGCAAAAAGGCATCGGTGAATAATTTACCTACAG 6 0.15 No Hit TGTGTTGATATAGAAAACAATGGCACTACATGGAAAGATTGAGACAACAT 6 0.15 No Hit CAGAAGTTGAGAGAGGTACTAAGATTTGCAAATGCATGTGGAGCAATCAT 6 0.15 No Hit CAACTTCTGTTAATTCTTCGTAGATATGGATATCGTGAGAAGGTTGGTTG 6 0.15 No Hit CCCCGCTTGAAATGTGGCGGCGGCAGTACTGCGCAGTTCCATGCCAGCCG 6 0.15 No Hit GTTTTCGTTTTCAAGAAAACACCATGAGCGATCAGAGGCAAACAGGGAAG 6 0.15 No Hit GATAAGCTAGGCAAGATGGTTTTACTAGACAAGATGTGGGATGATGTTGT 6 0.15 No Hit GGAGAAGGAAGGGCTTCTACAACTTCCATCTGACAAGGCTCTTCTATCTG 6 0.15 No Hit AAACAAGCAATTTAGGCCAGGCCACGTCTATAGCTAGATTTGGCTTGGTT 6 0.15 No Hit GTACAACACAGCTGAACATGCTTAAAGCCTCGGTGTGGGAGAAGCATTCT 6 0.15 No Hit CAGTCTTAACAACTACTTGCAAGTGAAGGCTGTCGTTACCTCACTCAAGA 5 0.125 No Hit GACAACAAGAAATGGAGAGGAATGCAGCCGTGAGCAAGCCACACACTTCC 5 0.125 No Hit GCACAAGCGAAAGCAGAAAGAAGAGAAAACAGCAATGGAAGAGGAAATGG 5 0.125 No Hit GAGAGAGACCAGCTTCTTCGGATGGCTAAGAGCTTGAATCCAAAACTAGT 5 0.125 No Hit ATGGAACGTTCAGCCTGTCAAATGTCCAGTGACTGTCTGTGGTGATATTC 5 0.125 No Hit GTGGACCAATGGGAACCAGTGTCATGTGATTCTGTGAAGACACCACTTGA 5 0.125 No Hit ACTCTACGATGCTGATGTCCTGGAGGAAGAATACATTGTGCAGTGGTATC 5 0.125 No Hit CGGACCCCCATGGGACGTGCTGGAGAACCAGGAGAAGTCTCTTCTGTTGT 5 0.125 No Hit ATGAAGCCAGAGTCTGCTTTGCTTTATCTGGAGCTTCCATCCAGTGTCTT 5 0.125 No Hit GATAAATAAGACTGATCCGTTGGTAGCTCCTTCGGGCATGGAGATCATTG 5 0.125 No Hit CTTTGATCGAGATAAATTTCATGAAGTGGTTCATGACACCGAGGCTGTAG 5 0.125 No Hit GGAAAGGGGTGGCGGCTGAGCGAAGCATTGCCGCTTTAAAGAATCATAGT 5 0.125 No Hit GAGAGCGAGCCGCTAGCTAGCTGCTCTGCAGGACGCTGTTCTTCTTGAGA 5 0.125 No Hit GAGGAGAGACACACACACAGAATTCTCCCTCTCTCAAAAAGTTGGCATTT 5 0.125 No Hit CGAGAACATTTCTCGCAACTGGTATTTTGGCAATGACCGATCGATGCTTG 5 0.125 No Hit AAAGGATTAGAAGGTTTTCCTGTGGCTGCTAGGTTCTGACGCAAATTTGC 5 0.125 No Hit GGATGGGACCCGGTGAAGTACGAGCCTCTGAGTCAAGTCTTTGTTTACGA 5 0.125 No Hit GGACCGTTTTGCAAACTATAATCTTGGTGTGGGTTACTTTGAGAACAGAT 5 0.125 No Hit GCCAACTGTACCTGCGATCCTTGCACTTGTAAATGAGAGCGATCCGCTGG 5 0.125 No Hit GTTCTGTTGGTGCTGGTGAATGCTGGCACAAACATGGCAGCTTTCTTGAT 5 0.125 No Hit CGTAAACAGCCTGGTAATCGGTTACCAGTTCAGGCTGTTAAGTTCTTTGC 5 0.125 No Hit AATGAGGGAAATTATGACAGCCCAAGCAACTTCTTGTGATCTCAAGGAAT 5 0.125 No Hit GATCCTCCGAAGCTCAACCGTTGTACGGAGTTGCTGTCTATGCAAGAGGA 5 0.125 No Hit CGGCAATGCTATTACAGATAGCTTCTGCGCCGCTCAAAAGACCGCGTTCG 5 0.125 No Hit CTTTAGCTGAGCCGGAAAGGGAAGAGAGTAGAACAAATCAGAGAGCACAG 5 0.125 No Hit GCTGGGGACATATGAAGATAATAGGTACAAGTCAGATTCAAAGAAACCTG 5 0.125 No Hit GTGTGATCAAGTGTGTAGGCTTTGTTTTGACACCTGGCATTTTTGCAGCT 5 0.125 No Hit CTTTTCCGCGGTGGCTGGACGATGCGTAAATTCTCGGCGACTGTCGTCAA 5 0.125 No Hit GATACATCAAGCTATGGGGACTGGAGTGGATGTCACTTTTGATTGTGCAG 5 0.125 No Hit GTACAAGAAAGGTGATGGTTTCGTTGAAGGCACAGAGTGCTCAGTTTGTC 5 0.125 No Hit ATTAGAGCAATGGGCTGCGTGTACTTTTCCTCACCTTCAAAAATTGAAGA 5 0.125 No Hit CTTCAAACCAATTCCCTTTTTGGTGGTTCGGTTCCACCATCAAATCCCGT 5 0.125 No Hit GCGATCATGAGTGTGATCCTGAAGAACCAGGTTCATGTGACTCTGGATGC 5 0.125 No Hit ACGAGAACTACTCCGAAGCTGAAGACATCACTCCTTACTGTAAAATTACC 5 0.125 No Hit TGGGTGACCATTTCAAAGGCGCAGGGGACAGCGTCGCATCGACGTTCCAA 5 0.125 No Hit GCCTGTTCATCAGCAGCAGCATTATGACCTAGACTAGCAGATTCACCACA 5 0.125 No Hit GTTTGCTCAGATTGAAGGAGCCTGCTGTCTTGCTACGTTGCCGGAAAGAT 5 0.125 No Hit GCCGATGTAACTAAGGGGTCTGACAAGCTAGTACAGGCCATAGGGGATGA 5 0.125 No Hit AGAAAACCCCTAATAATCTGAATCTCTACTTGAAGGATCAGAGAGTGATG 5 0.125 No Hit TGTGCATGCATCGAATGAAGATAGTTCATCGGGATCTTAAAAGTGCAAAT 5 0.125 No Hit AGAGAGTGCAATGGTCACATATCAGTTGGTATTTGGTAAGAATGAGATGG 5 0.125 No Hit CAGACCCTGCTTTTCTTGTCTCCAAGAAATCTCTGTCCCTTTCCTTGCCG 5 0.125 No Hit ATCAAAACCTTAGCCTTGGATGATGATACAAATGAGCAGACAACAACCAT 5 0.125 No Hit AGGATATCAAGCTGAAACGCTGGCGCGTTTTCTAAAAGCTAGAGAATGGA 5 0.125 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0125 0.0 0.0 0.0 0.0 34-35 0.025 0.0 0.0 0.0 0.0 36-37 0.025 0.0 0.0 0.0 0.0 38-39 0.025 0.0 0.0 0.0 0.0 40-41 0.025 0.0 0.0 0.0 0.0 42-43 0.025 0.0 0.0 0.0 0.0 44-45 0.025 0.0 0.0 0.0 0.0 46-47 0.025 0.0 0.0 0.0 0.0 48-49 0.025 0.0 0.0 0.0 0.0 50-51 0.025 0.0 0.0 0.0 0.0 52-53 0.025 0.0 0.0 0.0 0.0 54-55 0.025 0.0 0.0 0.0 0.0 56-57 0.025 0.0 0.0 0.0 0.0 58-59 0.025 0.0 0.0 0.0 0.0 60-61 0.025 0.0 0.0 0.0 0.0 62-63 0.025 0.0 0.0 0.0 0.0 64-65 0.025 0.0 0.0 0.0 0.0 66-67 0.025 0.0 0.0 0.0 0.0 68-69 0.025 0.0 0.0 0.0 0.0 70-71 0.025 0.0 0.0 0.0 0.0 72-73 0.025 0.0 0.0 0.0 0.0 74-75 0.1 0.0 0.0 0.0 0.0 76-77 0.175 0.0 0.0 0.0 0.0 78-79 0.175 0.0 0.0 0.0 0.0 80-81 0.1875 0.0 0.0 0.0 0.0 82-83 0.225 0.0 0.0 0.0 0.0 84-85 0.36250000000000004 0.0 0.0 0.0 0.0 86-87 0.4375 0.0 0.0 0.0 0.0 88-89 0.6375 0.0 0.0 0.0 0.0 90-91 0.8625 0.0 0.0 0.0 0.0 92-93 0.95 0.0 0.0 0.0 0.0 94-95 1.0375 0.0 0.0 0.0 0.0 96-97 1.1375000000000002 0.0 0.0 0.0 0.0 98-99 1.325 0.0 0.0 0.0 0.0 100-101 1.675 0.0 0.0 0.0 0.0 102-103 1.925 0.0 0.0 0.0 0.0 104-105 2.35 0.0 0.0 0.0 0.0 106-107 2.9875 0.0 0.0 0.0 0.0 108-109 3.5125 0.0 0.0 0.0 0.0 110-111 3.9250000000000003 0.0 0.0 0.0 0.0 112-113 4.425000000000001 0.0 0.0 0.0 0.0 114-115 4.925000000000001 0.0 0.0 0.0 0.0 116-117 5.675000000000001 0.0 0.0 0.0 0.0 118-119 6.387499999999999 0.0 0.0 0.0 0.0 120-121 6.925 0.0 0.0 0.0 0.0 122-123 7.7875 0.0 0.0 0.0 0.0 124-125 8.3625 0.0 0.0 0.0 0.0 126-127 8.9 0.0 0.0 0.0 0.0 128-129 9.7625 0.0 0.0 0.0 0.0 130-131 10.5375 0.0 0.0 0.0 0.0 132-133 11.45 0.0 0.0 0.0 0.0 134-135 12.175 0.0 0.0 0.0 0.0 136-137 12.8875 0.0 0.0 0.0 0.0 138-139 13.6125 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GCTGTAT 10 0.006830828 145.0 5 AGCTGTA 15 1.1411342E-4 145.0 4 AAGCTGT 10 0.006830828 145.0 3 >>END_MODULE Read 1695614 spots for SRR26075353.sra Written 1695614 spots for SRR26075353.sra Read 1695614 spots for SRR26075353.sra Written 1695614 spots for SRR26075353.sra Read 1695614 spots for SRR26075353.sra Written 1695614 spots for SRR26075353.sra Read 1695614 spots for SRR26075353.sra Written 1695614 spots for SRR26075353.sra Read 1695614 spots for SRR26075353.sra Written 1695614 spots for SRR26075353.sra Read 1695614 spots for SRR26075353.sra Written 1695614 spots for SRR26075353.sra Read 1695614 spots for SRR26075353.sra Written 1695614 spots for SRR26075353.sra Read 1695614 spots for SRR26075353.sra Written 1695614 spots for SRR26075353.sra Read 1695614 spots for SRR26075353.sra Written 1695614 spots for SRR26075353.sra Read 1695614 spots for SRR26075353.sra Written 1695614 spots for SRR26075353.sra Read 1695614 spots for SRR26075353.sra Written 1695614 spots for SRR26075353.sra Read 1695614 spots for SRR26075353.sra Written 1695614 spots for SRR26075353.sra Read 1695614 spots for SRR26075353.sra Written 1695614 spots for SRR26075353.sra Read 1695614 spots for SRR26075353.sra Written 1695614 spots for SRR26075353.sra Read 1695614 spots for SRR26075353.sra Written 1695614 spots for SRR26075353.sra Read 1695614 spots for SRR26075353.sra Written 1695614 spots for SRR26075353.sra Read 1695614 spots for SRR26075353.sra Written 1695614 spots for SRR26075353.sra Read 1695621 spots for SRR26075353.sra Written 1695621 spots for SRR26075353.sra Read 1695614 spots for SRR26075353.sra Written 1695614 spots for SRR26075353.sra Read 1695614 spots for SRR26075353.sra Written 1695614 spots for SRR26075353.sra SRR ids: ['SRR26075353.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_aw8wv476 SRR26075353.sra spots: 33912287 blocks: [[1, 1695614], [1695615, 3391228], [3391229, 5086842], [5086843, 6782456], [6782457, 8478070], [8478071, 10173684], [10173685, 11869298], [11869299, 13564912], [13564913, 15260526], [15260527, 16956140], [16956141, 18651754], [18651755, 20347368], [20347369, 22042982], [22042983, 23738596], [23738597, 25434210], [25434211, 27129824], [27129825, 28825438], [28825439, 30521052], [30521053, 32216666], [32216667, 33912287]] SRR26075353 file size 12522963 SRR26075353 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o SRR26075353 SRR26075353_1.fastq SRR26075353_2.fastq Input file: SRR26075353_1.fastq Paired file: SRR26075353_2.fastq trimmed: SRR26075353-trimmed-pair1.fastq, SRR26075353-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Wed Feb 12 02:52:27 2025 >> started Wed Feb 12 02:53:24 2025 >> done (56.886s) 33912287 read pairs processed; of these: 250 ( 0.00%) short read pairs filtered out after trimming by size control 485914 ( 1.43%) empty read pairs filtered out after trimming by size control 33426123 (98.57%) read pairs available; of these: 5899741 (17.65%) trimmed read pairs available after processing 27526382 (82.35%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 18 18 0.00% 19 19 0.00% 20 26 0.00% 21 30 0.00% 22 35 0.00% 23 31 0.00% 24 54 0.00% 25 47 0.00% 26 53 0.00% 27 51 0.00% 28 62 0.00% 29 76 0.00% 30 57 0.00% 31 76 0.00% 32 80 0.00% 33 59 0.00% 34 82 0.00% 35 90 0.00% 36 76 0.00% 37 102 0.00% 38 85 0.00% 39 92 0.00% 40 80 0.00% 41 87 0.00% 42 137 0.00% 43 126 0.00% 44 113 0.00% 45 171 0.00% 46 172 0.00% 47 224 0.00% 48 233 0.00% 49 214 0.00% 50 267 0.00% 51 316 0.00% 52 300 0.00% 53 333 0.00% 54 381 0.00% 55 414 0.00% 56 472 0.00% 57 534 0.00% 58 628 0.00% 59 713 0.00% 60 813 0.00% 61 879 0.00% 62 1032 0.00% 63 1196 0.00% 64 1368 0.00% 65 1348 0.00% 66 1555 0.00% 67 1844 0.01% 68 2148 0.01% 69 2317 0.01% 70 2700 0.01% 71 3388 0.01% 72 3728 0.01% 73 4255 0.01% 74 4803 0.01% 75 5372 0.02% 76 5985 0.02% 77 6615 0.02% 78 7294 0.02% 79 8491 0.03% 80 9570 0.03% 81 10672 0.03% 82 12317 0.04% 83 13573 0.04% 84 14947 0.04% 85 16738 0.05% 86 17851 0.05% 87 19239 0.06% 88 20737 0.06% 89 22213 0.07% 90 23649 0.07% 91 25703 0.08% 92 28592 0.09% 93 31465 0.09% 94 33731 0.10% 95 36534 0.11% 96 38305 0.11% 97 39819 0.12% 98 41321 0.12% 99 44358 0.13% 100 46094 0.14% 101 48261 0.14% 102 50253 0.15% 103 53663 0.16% 104 57192 0.17% 105 60352 0.18% 106 62830 0.19% 107 63795 0.19% 108 65843 0.20% 109 68150 0.20% 110 70068 0.21% 111 72040 0.22% 112 74663 0.22% 113 76416 0.23% 114 81122 0.24% 115 84368 0.25% 116 86258 0.26% 117 89433 0.27% 118 90620 0.27% 119 91346 0.27% 120 93421 0.28% 121 96243 0.29% 122 97828 0.29% 123 101755 0.30% 124 105749 0.32% 125 108106 0.32% 126 109984 0.33% 127 111828 0.33% 128 114654 0.34% 129 115549 0.35% 130 116398 0.35% 131 116945 0.35% 132 120207 0.36% 133 123320 0.37% 134 124035 0.37% 135 128951 0.39% 136 132196 0.40% 137 131075 0.39% 138 135292 0.40% 139 138030 0.41% 140 135691 0.41% 141 138683 0.41% 142 139930 0.42% 143 141435 0.42% 144 147175 0.44% 145 148261 0.44% 146 148772 0.45% 147 152372 0.46% 148 150968 0.45% 149 151899 0.45% 150 154541 0.46% 151 27526382 82.35% 33426123 reads passed initial QC criterion=sequence-density sequence-density=0.38 sequence-density-rank=1 fanout-score=2.28 fanout-score-rank=33 prefix-density=0.37 prefix-fanout=2.3 sequence=GTCAGGGTACAT criterion=fanout-score sequence-density=0.03 sequence-density-rank=37 fanout-score=294.86 fanout-score-rank=1 prefix-density=0.77 prefix-fanout=12.1 sequence=CCACCACCACCTCCACCACTTCCGCGGGATTGAGCTTCGTTCACGGTGATGTTACGGCCATCGAGGTCCTGACCGTTCATTCCATCAATAGCATCTCTCATTGCCTTCTC criterion=sequence-density sequence-density=0.63 sequence-density-rank=1 fanout-score=2.37 fanout-score-rank=23 prefix-density=0.64 prefix-fanout=2.3 sequence=ATGTACCCTGAC criterion=fanout-score sequence-density=0.01 sequence-density-rank=35 fanout-score=167.70 fanout-score-rank=1 prefix-density=0.14 prefix-fanout=6.9 sequence=GAGAAAAGATAAGCTAGGCAAGATGGTTTTACTAGACAAGATGTGGGATGATGTTGTTGCTGGACCTCAGCCAGAACGTGGCCTTGGCAAGCTTAGAAAGATCAGCACCAGACCACTTAACATCAAAGATATTGACGTCGGAGAGGGGAGCAGTCCTGTTAATAAGTTTCAGAGGTCCATGACTATGCCAGGAACTCCAGGGACACCGACGACACCAGTGACCCCTACAACCCCAGTGTCGGCGCGTAGCAATGTTTGGAGGAGCGTGTTCCACCCTGGTAGCAACCTTGCTACTAAGAATATTGGTGCTCATGTTTTTGACAAGCCACAGCCTAACACACCCACTGTCTATGACTGGATGTACAGTGGAGAGACGAAGAGCGAGCATCGTTGATGAGGTTGCCTTCAACCAAGGTTGCCCATGTAAATACGTACTGTGTTTTGTTTTTCAGTACT SRR26075353 testing PE reads STAR mapping to Ensembl genome Started job on | Feb 12 02:54:08 Started mapping on | Feb 12 02:54:08 Finished on | Feb 12 03:01:19 Mapping speed, Million of reads per hour | 279.20 Number of input reads | 33426123 Average input read length | 293 UNIQUE READS: Uniquely mapped reads number | 28602271 Uniquely mapped reads % | 85.57% Average mapped length | 291.40 Number of splices: Total | 25803631 Number of splices: Annotated (sjdb) | 25141609 Number of splices: GT/AG | 25317285 Number of splices: GC/AG | 368236 Number of splices: AT/AC | 29398 Number of splices: Non-canonical | 88712 Mismatch rate per base, % | 0.41% Deletion rate per base | 0.03% Deletion average length | 3.04 Insertion rate per base | 0.02% Insertion average length | 2.44 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 845591 % of reads mapped to multiple loci | 2.53% Number of reads mapped to too many loci | 94385 % of reads mapped to too many loci | 0.28% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 11.08% % of reads unmapped: other | 0.53% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 3978261 3978261 3978261 N_multimapping 845591 845591 845591 N_noFeature 763982 28256599 957067 N_ambiguous 323675 1748 169842 UnstrandedReadsAssigned:27514614 PositiveStrandReadsAssigned:343924 NegativeStrandReadsAssigned:27475362 Dataset is classified negative stranded MeadianReadLen=151 20thPercentileLength=151 echo kmer=147 SRR26075353 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,400 [index] number of k-mers: 62,057,036 [index] number of equivalence classes: 130,681 [quant] running in paired-end mode [quant] will process pair 1: SRR26075353-trimmed-pair1.fastq SRR26075353-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 33,426,123 reads, 28,029,770 reads pseudoaligned [quant] estimated average fragment length: 208.494 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,229 rounds 52401 SRR26075353.ke.tsv 34699 SRR26075353.se.tsv 87100 total ==> SRR26075353.ke.tsv <== target_id length eff_length est_counts tpm Potri.005G200100.1.v4.1 2018 1810.51 3289 53.9716 Potri.005G024800.1.v4.1 1035 827.506 3750 134.636 Potri.004G059700.1.v4.1 961 753.516 0 0 Potri.007G009000.2.v4.1 1416 1208.51 0 0 Potri.003G141000.2.v4.1 2943 2735.51 1222 13.2719 Potri.016G087400.1.v4.1 270 92.7747 1907 610.692 Potri.015G069301.1.v4.1 564 358.048 0 0 Potri.010G195200.1.v4.1 1773 1565.51 1207 22.9062 Potri.012G127500.1.v4.1 977 769.511 20020 772.948 ==> SRR26075353.se.tsv <== Potri.001G166300.v4.1 0 Potri.001G448400.v4.1 69 Potri.001G233950.v4.1 0 Potri.001G122700.v4.1 438 Potri.001G212900.v4.1 0 Potri.001G182400.v4.1 0 Potri.001G256600.v4.1 0 Potri.001G040500.v4.1 8 Potri.001G416900.v4.1 0 Potri.001G452600.v4.1 2235 SRR26075353 completed mapping pipeline successfully