Starting /dee2/code/volunteer_pipeline.sh SRR26075354 current disk space = 3052627419136 free memory = 1475803180 SRR26075354 SRAfilesize bf26fcc630fc813375faddca6cbdfc04 SRR26075354.sra SRR26075354.sra file validated SRR26075354 is paired end SRR26075354 is conventional basespace SRR26075354 read1 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR26075354_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 49 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.50225 37.0 37.0 37.0 37.0 37.0 2 36.644 37.0 37.0 37.0 37.0 37.0 3 36.6365 37.0 37.0 37.0 37.0 37.0 4 36.727 37.0 37.0 37.0 37.0 37.0 5 36.7165 37.0 37.0 37.0 37.0 37.0 6 36.6205 37.0 37.0 37.0 37.0 37.0 7 36.6155 37.0 37.0 37.0 37.0 37.0 8 36.5425 37.0 37.0 37.0 37.0 37.0 9 36.626 37.0 37.0 37.0 37.0 37.0 10-14 36.619699999999995 37.0 37.0 37.0 37.0 37.0 15-19 36.5517 37.0 37.0 37.0 37.0 37.0 20-24 36.506299999999996 37.0 37.0 37.0 37.0 37.0 25-29 36.400800000000004 37.0 37.0 37.0 37.0 37.0 30-34 36.3882 37.0 37.0 37.0 37.0 37.0 35-39 36.330400000000004 37.0 37.0 37.0 37.0 37.0 40-44 36.0236 37.0 37.0 37.0 37.0 37.0 45-49 34.427699999999994 37.0 37.0 37.0 24.2 37.0 50-54 33.6086 37.0 37.0 37.0 16.6 37.0 55-59 33.159800000000004 37.0 37.0 37.0 11.0 37.0 60-64 33.3661 37.0 37.0 37.0 13.8 37.0 65-69 33.1217 37.0 37.0 37.0 13.8 37.0 70-74 34.0634 37.0 37.0 37.0 19.4 37.0 75-79 35.7658 37.0 37.0 37.0 37.0 37.0 80-84 35.902300000000004 37.0 37.0 37.0 37.0 37.0 85-89 35.847699999999996 37.0 37.0 37.0 37.0 37.0 90-94 35.7494 37.0 37.0 37.0 37.0 37.0 95-99 35.7646 37.0 37.0 37.0 37.0 37.0 100-104 35.740700000000004 37.0 37.0 37.0 37.0 37.0 105-109 35.5726 37.0 37.0 37.0 37.0 37.0 110-114 35.4904 37.0 37.0 37.0 37.0 37.0 115-119 35.3211 37.0 37.0 37.0 34.6 37.0 120-124 35.30069999999999 37.0 37.0 37.0 34.6 37.0 125-129 35.0601 37.0 37.0 37.0 27.4 37.0 130-134 34.7359 37.0 37.0 37.0 25.0 37.0 135-139 34.5841 37.0 37.0 37.0 25.0 37.0 140-144 34.2315 37.0 37.0 37.0 25.0 37.0 145-149 33.988 37.0 37.0 37.0 25.0 37.0 150-151 33.839 37.0 37.0 37.0 25.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.0 1101 2 0.0 1101 3 0.0 1101 4 0.0 1101 5 0.0 1101 6 0.0 1101 7 0.0 1101 8 0.0 1101 9 0.0 1101 10-14 0.0 1101 15-19 0.0 1101 20-24 0.0 1101 25-29 0.0 1101 30-34 0.0 1101 35-39 0.0 1101 40-44 0.0 1101 45-49 0.0 1101 50-54 0.0 1101 55-59 0.0 1101 60-64 0.0 1101 65-69 0.0 1101 70-74 0.0 1101 75-79 0.0 1101 80-84 0.0 1101 85-89 0.0 1101 90-94 0.0 1101 95-99 0.0 1101 100-104 0.0 1101 105-109 0.0 1101 110-114 0.0 1101 115-119 0.0 1101 120-124 0.0 1101 125-129 0.0 1101 130-134 0.0 1101 135-139 0.0 1101 140-144 0.0 1101 145-149 0.0 1101 150-151 0.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 22 3.0 23 3.0 24 4.0 25 11.0 26 19.0 27 17.0 28 21.0 29 37.0 30 43.0 31 73.0 32 494.0 33 274.0 34 222.0 35 398.0 36 2241.0 37 140.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.11838475043893 5.242036619011788 11.98896413343366 38.650614497115626 2 20.674999999999997 17.849999999999998 31.2 30.275000000000002 3 20.65 15.024999999999999 37.125 27.200000000000003 4 23.400000000000002 18.8 23.65 34.150000000000006 5 35.85 20.775 25.55 17.825 6 35.025 22.25 24.125 18.6 7 13.8 34.475 39.6 12.125 8 18.175 29.725 30.049999999999997 22.05 9 29.175 18.224999999999998 34.25 18.35 10-14 20.927092709270926 30.098009800980098 26.36263626362636 22.612261226122612 15-19 21.64 26.650000000000002 28.315 23.395 20-24 21.105 29.23 27.589999999999996 22.075 25-29 21.14 26.534999999999997 27.715 24.610000000000003 30-34 18.64 26.265 27.584999999999997 27.51 35-39 21.45 26.865 28.854999999999997 22.830000000000002 40-44 18.89 29.25 27.355 24.505 45-49 24.75 24.104999999999997 28.53 22.615 50-54 25.6 24.13 28.395 21.875 55-59 27.815 24.905 27.145000000000003 20.135 60-64 28.294999999999998 24.575 27.315 19.814999999999998 65-69 24.73 26.6 28.305000000000003 20.365 70-74 30.845 25.074999999999996 24.085 19.994999999999997 75-79 31.135 24.86 24.545 19.46 80-84 31.55 23.79 24.875 19.785 85-89 31.04 25.1 23.865 19.994999999999997 90-94 31.514999999999997 24.335 24.575 19.575 95-99 31.605 24.565 23.845 19.985 100-104 31.615 23.94 23.915 20.53 105-109 31.335 24.51 24.135 20.02 110-114 31.685000000000002 24.935 23.325000000000003 20.055 115-119 31.595000000000002 24.585 23.115 20.705000000000002 120-124 32.574999999999996 24.645 22.085 20.695 125-129 32.545 25.0 21.9 20.555 130-134 32.82 24.310000000000002 22.615 20.255000000000003 135-139 33.43 23.845 22.1 20.625 140-144 33.989999999999995 23.685000000000002 21.945 20.380000000000003 145-149 34.57 23.485 21.215 20.73 150-151 34.625 22.6375 21.6625 21.075 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1.0 1 0.5 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.5 9 0.5 10 0.0 11 0.0 12 0.0 13 0.0 14 0.5 15 1.0 16 0.5 17 0.5 18 2.0 19 2.0 20 0.5 21 1.0 22 3.5 23 4.0 24 5.5 25 6.5 26 8.0 27 10.0 28 9.0 29 15.0 30 22.0 31 25.5 32 35.0 33 46.5 34 50.5 35 57.0 36 78.5 37 100.5 38 108.5 39 121.0 40 160.5 41 187.0 42 203.0 43 210.0 44 186.5 45 201.5 46 225.5 47 209.5 48 191.5 49 186.0 50 156.0 51 107.5 52 90.0 53 92.5 54 79.5 55 58.0 56 54.5 57 44.0 58 29.0 59 26.5 60 24.5 61 22.0 62 14.5 63 10.5 64 8.5 65 5.0 66 5.0 67 5.0 68 11.0 69 23.5 70 41.0 71 65.0 72 76.5 73 77.0 74 74.5 75 57.5 76 35.0 77 16.5 78 6.0 79 4.0 80 2.5 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.325 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.01 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150-151 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 57.85 #Duplication Level Percentage of deduplicated Percentage of total 1 67.54537597234227 39.074999999999996 2 19.490060501296455 22.55 3 7.908383751080381 13.725000000000001 4 2.4632670700086434 5.7 5 1.166810717372515 3.375 6 0.6050129645635264 2.1 7 0.17286084701815038 0.7000000000000001 8 0.08643042350907519 0.4 9 0.216076058772688 1.125 >10 0.17286084701815038 1.225 >50 0.08643042350907519 3.025 >100 0.08643042350907519 7.000000000000001 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTATCGCAATCTCGGTT 150 3.75 TruSeq Adapter, Index 2 (97% over 37bp) GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTATCGCAATCTCGGGT 130 3.25 TruSeq Adapter, Index 2 (97% over 37bp) GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTATCGCAATCGCGGGT 65 1.625 TruSeq Adapter, Index 2 (97% over 37bp) GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTATCGCAATCGCGGTT 56 1.4000000000000001 TruSeq Adapter, Index 2 (97% over 37bp) GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTATCGCAATCTCGTAT 15 0.375 TruSeq Adapter, Index 2 (97% over 37bp) GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTATCGCAATCTCGGGG 12 0.3 TruSeq Adapter, Index 2 (97% over 37bp) CTCCGCCTCGACAAGCTCCCAAATCCATCCCTCATGTCACGAACTATACT 12 0.3 No Hit GTCGAAATCAGACATGAAAACTTAACTGTTCAAGGAAGTCCAACAACCTG 10 0.25 No Hit ACAGCTATACCAAATCCATCAGAGGAATATTACCTAGTGAGATAGTATAT 9 0.22499999999999998 No Hit GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTATCGCAATCTCGTTT 9 0.22499999999999998 TruSeq Adapter, Index 2 (97% over 37bp) GTCTGTATGTATAGTTGTATATTGAAAGATAAAGATGCAGCATGGGACAA 9 0.22499999999999998 No Hit GTACTGATTCATCTGCTGGTTCACAGCTAATGTGGTTGCAACGGCTCCTT 9 0.22499999999999998 No Hit ATTGTGGCGGAGTAAAAATAGGGTTTTGGTTGAGGAGAGAAAGATGTGCC 9 0.22499999999999998 No Hit CTCCGCTCACGCTCTTCCAACTCCTCACGGAGATTCCTCTTCAGCAATTC 8 0.2 No Hit GTTTGGAGAAAGCGGATCGGTCGATTAGTTCGGTAAAGGGGCGGCGTTGG 8 0.2 No Hit GCAATGAGGGCATTTATCCTTCTCACTAATTAGCTCACCTGAGCCTCTGC 7 0.17500000000000002 No Hit GTGGAGATGGGTTTTGATCAGGACCGTTGCTTTGCTGCATCTTGATCTGA 7 0.17500000000000002 No Hit CTGTGCTCTTGTTGGTTGTACCCACCTTGTGCGCCTCCCCTGTGCTCTTG 7 0.17500000000000002 No Hit CCTTTATCATTTCCAGTCCCGACTGTACAATCTGACAGCATTATGGTTTC 7 0.17500000000000002 No Hit GTGGGTACTGAGCCTAGTGGTGGAGAGACTCGGAAATGATCAATGAGTTT 6 0.15 No Hit CCTCAGATGTGCTTGCTTCAGAAATGGACTTGCTTGTGTCCTTGTACTCA 6 0.15 No Hit GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTATCGCAATCTCGTGT 6 0.15 TruSeq Adapter, Index 2 (97% over 37bp) GCGGTGAGTTCATGCATCCAAATGGTAGCGGTGACGTTGGCAATTGAAAC 6 0.15 No Hit TTTTTGTGTGCCAATCAGAATCTTCCAACACCAACATACAAATCCTTTAT 6 0.15 No Hit TCCAGGTAATGCTGCCATTCCAGTTGGATCCAGATAAGATCCGTCAGCCG 6 0.15 No Hit AACGCCCGAAAGTCAACTGGTGTTATATAACAATAAAAGTCACAGCCTTT 6 0.15 No Hit CTCGGGCGTGGAGAGGGAGAGGGAGACCTTCATGCATTCAGTGGCATCAG 6 0.15 No Hit GGCTTTTCAGCGGCGAAGAAGGAGTCCACATCGGATTGGCGCGTGAGGTC 6 0.15 No Hit CCCAGATCTCTTCCTCCTTGGCTGGAACTGCAGTGATTTTGTTTTTAGGA 6 0.15 No Hit TGGACTTAATTAATGAGTCCTTTTCTGCAACTTGGAGTTTGACTTCATCG 6 0.15 No Hit CTCATCTTCTGACTTGTACTTCTCGGCCTCCTGGACCATCTTCTCAATGT 6 0.15 No Hit CACCGGATAATCCTTTCCCAACAGCAACCTCATGTATGGTTTCATCAGGA 6 0.15 No Hit TGGGGGAAGATAGGGACGGCTGAGAGGGAGAAAAGAGGCTTGTGTGCTCT 6 0.15 No Hit GTAGAGAATTGCTTGGGACATCTAGCTCTTCACCAGGAAGTGATCCAGAT 5 0.125 No Hit CAGAACATAATCCCCCCCCTGTAAGGTGGAAAACCCATTCCCATTAGAGA 5 0.125 No Hit GGTGAATTGGGGTCTTTGTAAATGCAGCTGCTGCTTTCCATTGGTCTGAT 5 0.125 No Hit CCACTGCTGGGGTGAGTTGACTACTGTTGCATTTCTGTTGTTCTGGCGCC 5 0.125 No Hit GCAGGGAAGAGAAGAAGAAGGAAACAGATAGTATATGGAAGATTAAGGGT 5 0.125 No Hit AGGGCAAATAAGATGATTGAAGAGATGAAGGAGAGGTGGATCCCAGAGAG 5 0.125 No Hit GGAGCGGACAAGAAAGGAAAAATCTCTACAGACTGAAAAGACATGGACTG 5 0.125 No Hit GGTGGGGGCAATGAGGTCATGGACTCTGCAGGTGCAGACATTGGCGGTGG 5 0.125 No Hit CTGTCATCTTGGCCATCGACTTCAGATTTGGACCTGTGAGAATGTTTGTA 5 0.125 No Hit CGAACTGCTCGGCCAGGACTTTCCATAAATGCTTCCACTTGAAGTAATCT 5 0.125 No Hit CTAACTATTTCGAGGAGGACAACTCCGAAACTGTAAACATCAGCTTTAAC 5 0.125 No Hit CTATTGGATATTCATCACCATGAAACCTGGAGGTTTTTATAACTTGACAT 5 0.125 No Hit GTCGGTTTGTTTTTCTTTGAGGAGAAGGGCCTGTGTTTTGTGGGGTTCTC 5 0.125 No Hit GTTAAAGGGAAAATGAGGGCTGGTCTTGGTCGGCTTGCCCGGCTGAAGGG 5 0.125 No Hit TTTTTTGGCAAAGAGCACTAGCAATTAACCTCAATAAACACAACCACAAG 5 0.125 No Hit GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTATCGCAATCGCGGGG 5 0.125 TruSeq Adapter, Index 2 (97% over 37bp) GCAAGGCTGAAATCTTTGCGAACATAATAATACGAGAAGTTGCCGAATCC 5 0.125 No Hit GGATTTGATTTTTAGGGTTTAAAAGGACAGAGAGAGAAAGGGGAAATCTA 5 0.125 No Hit GCTCCAGGGAGCTTATTTTTCCATTCATAAGCAGATTTCTGGAACCAGCC 5 0.125 No Hit GTGTCTGTCTTTGTTTGAGCTTCTCTTTGGTGCTAACTGTTTCTTTTGCT 5 0.125 No Hit TTTTTCTGCTAACAAGAAGCTCAATATTATCAGATTGAAAGAAATGTATC 5 0.125 No Hit GCTCTTCTCAGCTAGCGCACATTATTGAGCACACATTTTTTTTTGGGCTA 5 0.125 No Hit CCCCCATCTGGGACTGATCACCCTTTAAAGATGTCTTTCCACATGCAAAC 5 0.125 No Hit CGGATGCGAATAATAAACAAGAGCTTAGCCTCAGGGTCAACATAAAATCC 5 0.125 No Hit GGGGTCTACTGGTGGAGGCCGGTCTGTGAACCGGTGGGTGTTGGTCTGGC 5 0.125 No Hit CCATTATCTACCGTATGAAGATCCTTAACATTAAAAGACGGGTAAGGATG 5 0.125 No Hit GGTAGAATAAGATTGATGTTGAGAAGCTTAGCCTTGATAACGGTGCAAAG 5 0.125 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.05 0.0 0.0 0.0 0.0 2 0.05 0.0 0.0 0.0 0.0 3 0.05 0.0 0.0 0.0 0.0 4 0.05 0.0 0.0 0.0 0.0 5 0.05 0.0 0.0 0.0 0.0 6 0.05 0.0 0.0 0.0 0.0 7 0.05 0.0 0.0 0.0 0.0 8 0.05 0.0 0.0 0.0 0.0 9 0.05 0.0 0.0 0.0 0.0 10-11 0.05 0.0 0.0 0.0 0.0 12-13 0.05 0.0 0.0 0.0 0.0 14-15 0.05 0.0 0.0 0.0 0.0 16-17 0.05 0.0 0.0 0.0 0.0 18-19 0.05 0.0 0.0 0.0 0.0 20-21 0.05 0.0 0.0 0.0 0.0 22-23 0.05 0.0 0.0 0.0 0.0 24-25 0.05 0.0 0.0 0.0 0.0 26-27 0.05 0.0 0.0 0.0 0.0 28-29 0.05 0.0 0.0 0.0 0.0 30-31 0.05 0.0 0.0 0.0 0.0 32-33 0.05 0.0 0.0 0.0 0.0 34-35 0.05 0.0 0.0 0.0 0.0 36-37 0.05 0.0 0.0 0.0 0.0 38-39 0.0625 0.0 0.0 0.0 0.0 40-41 0.075 0.0 0.0 0.0 0.0 42-43 0.175 0.0 0.0 0.0 0.0 44-45 0.175 0.0 0.0 0.0 0.0 46-47 0.175 0.0 0.0 0.0 0.0 48-49 0.175 0.0 0.0 0.0 0.0 50-51 0.1875 0.0 0.0 0.0 0.0 52-53 0.2 0.0 0.0 0.0 0.0 54-55 0.2 0.0 0.0 0.0 0.0 56-57 0.21250000000000002 0.0 0.0 0.0 0.0 58-59 0.2625 0.0 0.0 0.0 0.0 60-61 0.3 0.0 0.0 0.0 0.0 62-63 0.5875 0.0 0.0 0.0 0.0 64-65 0.65 0.0 0.0 0.0 0.0 66-67 0.75 0.0 0.0 0.0 0.0 68-69 0.825 0.0 0.0 0.0 0.0 70-71 1.0 0.0 0.0 0.0 0.0 72-73 1.15 0.0 0.0 0.0 0.0 74-75 1.325 0.0 0.0 0.0 0.0 76-77 1.4874999999999998 0.0 0.0 0.0 0.0 78-79 1.7875 0.0 0.0 0.0 0.0 80-81 2.1 0.0 0.0 0.0 0.0 82-83 2.4875 0.0 0.0 0.0 0.0 84-85 3.05 0.0 0.0 0.0 0.0 86-87 3.3375 0.0 0.0 0.0 0.0 88-89 3.6375 0.0 0.0 0.0 0.0 90-91 4.05 0.0 0.0 0.0 0.0 92-93 4.7375 0.0 0.0 0.0 0.0 94-95 5.325 0.0 0.0 0.0 0.0 96-97 5.8 0.0 0.0 0.0 0.0 98-99 6.362500000000001 0.0 0.0 0.0 0.0 100-101 6.975 0.0 0.0 0.0 0.0 102-103 7.4125 0.0 0.0 0.0 0.0 104-105 8.075 0.0 0.0 0.0 0.0 106-107 8.5625 0.0 0.0 0.0 0.0 108-109 9.2625 0.0 0.0 0.0 0.0 110-111 9.837499999999999 0.0 0.0 0.0 0.0 112-113 10.787500000000001 0.0 0.0 0.0 0.0 114-115 11.925 0.0 0.0 0.0 0.0 116-117 12.787500000000001 0.0 0.0 0.0 0.0 118-119 13.5 0.0 0.0 0.0 0.0 120-121 14.4125 0.0 0.0 0.0 0.0 122-123 15.175 0.0 0.0 0.0 0.0 124-125 15.9125 0.0 0.0 0.0 0.0 126-127 16.9625 0.0 0.0 0.0 0.0 128-129 17.5875 0.0 0.0 0.0 0.0 130-131 18.6875 0.0 0.0 0.0 0.0 132-133 19.6 0.0 0.0 0.0 0.0 134-135 20.5 0.0 0.0 0.0 0.0 136-137 21.725 0.0 0.0 0.0 0.0 138-139 22.65 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GAGCACA 140 0.0 56.96429 9 AGAGCAC 140 0.0 56.96429 8 AAGAGCA 150 0.0 53.166668 7 GAAGAGC 155 0.0 51.451614 6 TCGGAAG 155 0.0 51.451614 3 CGGAAGA 160 0.0 49.84375 4 GGAAGAG 160 0.0 49.84375 5 GATCGGA 165 0.0 48.333336 1 ATCGGAA 165 0.0 48.333336 2 AGGGGGG 30 4.189703E-5 29.000002 65-69 GGTTGGC 45 8.383813E-7 25.777777 45-49 GTTGGCG 45 8.383813E-7 25.777777 45-49 TGGCGGG 45 8.383813E-7 25.777777 50-54 CGGTTGG 45 8.383813E-7 25.777777 45-49 TCGGTTG 35 1.1966578E-4 24.857143 45-49 CTCGGTT 35 1.1966578E-4 24.857143 40-44 TTGGCGG 50 2.0994885E-6 23.199999 45-49 TCTCGGT 40 2.9585467E-4 21.75 40-44 AATCTCG 70 1.807628E-6 18.642857 40-44 ATCTCGG 55 1.1668232E-4 18.454546 40-44 >>END_MODULE SRR26075354 read2 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR26075354_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 51 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.35475 37.0 37.0 37.0 37.0 37.0 2 36.5075 37.0 37.0 37.0 37.0 37.0 3 36.4135 37.0 37.0 37.0 37.0 37.0 4 36.427 37.0 37.0 37.0 37.0 37.0 5 36.3575 37.0 37.0 37.0 37.0 37.0 6 36.318 37.0 37.0 37.0 37.0 37.0 7 36.2085 37.0 37.0 37.0 37.0 37.0 8 36.063 37.0 37.0 37.0 37.0 37.0 9 36.144 37.0 37.0 37.0 37.0 37.0 10-14 35.9063 37.0 37.0 37.0 37.0 37.0 15-19 35.731 37.0 37.0 37.0 37.0 37.0 20-24 35.447199999999995 37.0 37.0 37.0 37.0 37.0 25-29 34.684900000000006 37.0 37.0 37.0 25.0 37.0 30-34 34.0998 37.0 37.0 37.0 25.0 37.0 35-39 33.5544 37.0 37.0 37.0 16.6 37.0 40-44 33.4032 37.0 37.0 37.0 13.8 37.0 45-49 33.106 37.0 37.0 37.0 11.0 37.0 50-54 32.6985 37.0 37.0 37.0 11.0 37.0 55-59 33.0264 37.0 37.0 37.0 11.0 37.0 60-64 33.4782 37.0 37.0 37.0 13.8 37.0 65-69 33.0849 37.0 37.0 37.0 11.0 37.0 70-74 32.7381 37.0 37.0 37.0 11.0 37.0 75-79 32.6019 37.0 37.0 37.0 11.0 37.0 80-84 32.819 37.0 37.0 37.0 11.0 37.0 85-89 33.3815 37.0 37.0 37.0 13.8 37.0 90-94 33.9092 37.0 37.0 37.0 25.0 37.0 95-99 34.5706 37.0 37.0 37.0 25.0 37.0 100-104 34.7387 37.0 37.0 37.0 25.0 37.0 105-109 34.807599999999994 37.0 37.0 37.0 25.0 37.0 110-114 34.958800000000004 37.0 37.0 37.0 25.0 37.0 115-119 34.9939 37.0 37.0 37.0 25.0 37.0 120-124 35.000249999999994 37.0 37.0 37.0 25.0 37.0 125-129 35.025600000000004 37.0 37.0 37.0 25.0 37.0 130-134 34.9294 37.0 37.0 37.0 25.0 37.0 135-139 34.7339 37.0 37.0 37.0 25.0 37.0 140-144 34.7477 37.0 37.0 37.0 25.0 37.0 145-149 34.6443 37.0 37.0 37.0 25.0 37.0 150-151 34.267 37.0 37.0 37.0 25.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.0 1101 2 0.0 1101 3 0.0 1101 4 0.0 1101 5 0.0 1101 6 0.0 1101 7 0.0 1101 8 0.0 1101 9 0.0 1101 10-14 0.0 1101 15-19 0.0 1101 20-24 0.0 1101 25-29 0.0 1101 30-34 0.0 1101 35-39 0.0 1101 40-44 0.0 1101 45-49 0.0 1101 50-54 0.0 1101 55-59 0.0 1101 60-64 0.0 1101 65-69 0.0 1101 70-74 0.0 1101 75-79 0.0 1101 80-84 0.0 1101 85-89 0.0 1101 90-94 0.0 1101 95-99 0.0 1101 100-104 0.0 1101 105-109 0.0 1101 110-114 0.0 1101 115-119 0.0 1101 120-124 0.0 1101 125-129 0.0 1101 130-134 0.0 1101 135-139 0.0 1101 140-144 0.0 1101 145-149 0.0 1101 150-151 0.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 12 1.0 13 5.0 14 13.0 15 11.0 16 12.0 17 15.0 18 10.0 19 13.0 20 14.0 21 19.0 22 28.0 23 37.0 24 57.0 25 87.0 26 108.0 27 116.0 28 70.0 29 31.0 30 25.0 31 31.0 32 42.0 33 68.0 34 169.0 35 587.0 36 2197.0 37 234.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 50.71267816954238 1.9504876219054765 20.305076269067268 27.031757939484873 2 46.125 6.425 25.75 21.7 3 32.475 16.425 33.425 17.675 4 35.85 23.825 20.075000000000003 20.25 5 37.075 28.1 17.0 17.825 6 31.900000000000002 32.550000000000004 18.6 16.950000000000003 7 33.75 19.75 30.3 16.2 8 34.275 21.575 21.75 22.400000000000002 9 33.0 22.325 23.775 20.9 10-14 34.12 25.369999999999997 22.425 18.085 15-19 34.06 24.490000000000002 23.055 18.395 20-24 32.824999999999996 24.485 23.89 18.8 25-29 33.375 25.324999999999996 23.22 18.08 30-34 32.895 25.235000000000003 23.425 18.445 35-39 33.15 25.215 23.865 17.77 40-44 33.425 25.509999999999998 22.770000000000003 18.295 45-49 32.574999999999996 25.619999999999997 24.01 17.794999999999998 50-54 22.515 25.395 33.06 19.03 55-59 30.485 24.585 26.974999999999998 17.955 60-64 33.839999999999996 24.675 23.200000000000003 18.285 65-69 33.445 25.86 23.32 17.375 70-74 27.3 30.575000000000003 23.745 18.38 75-79 24.975 31.0 25.380000000000003 18.645 80-84 30.404999999999998 27.235 23.985 18.375 85-89 33.565 26.169999999999998 23.369999999999997 16.895 90-94 33.42 25.840000000000003 22.695 18.045 95-99 33.875 25.935000000000002 22.09 18.099999999999998 100-104 34.760000000000005 25.705 22.31 17.224999999999998 105-109 34.585 26.200000000000003 22.17 17.044999999999998 110-114 34.415 26.045 22.5 17.04 115-119 35.025 25.715 22.07 17.19 120-124 35.75678783939197 26.136306815340767 21.576078803940195 16.530826541327066 125-129 35.335 24.63 22.985 17.05 130-134 36.445 25.45 21.75 16.355 135-139 36.22862286228623 26.117611761176118 21.382138213821385 16.27162716271627 140-144 36.5623124624925 26.07021404280856 21.424284856971397 15.943188637727545 145-149 37.07482993197279 25.075030012004802 21.988795518207283 15.861344537815125 150-151 36.384096024006 25.70642660665166 21.705426356589147 16.204051012753187 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.5 3 0.5 4 0.5 5 0.5 6 0.0 7 0.0 8 1.5 9 1.5 10 1.0 11 1.0 12 3.0 13 3.5 14 2.0 15 2.0 16 0.5 17 1.0 18 1.5 19 2.0 20 2.5 21 2.0 22 1.5 23 1.5 24 2.5 25 2.5 26 6.0 27 6.5 28 6.0 29 9.0 30 11.0 31 19.5 32 24.0 33 29.5 34 46.5 35 60.5 36 86.0 37 112.0 38 103.5 39 117.0 40 168.0 41 170.5 42 204.5 43 238.5 44 220.5 45 211.5 46 211.0 47 194.5 48 175.5 49 169.0 50 145.5 51 126.5 52 105.5 53 82.5 54 67.0 55 47.0 56 36.5 57 44.0 58 32.5 59 20.5 60 19.0 61 18.5 62 13.5 63 13.0 64 12.0 65 6.0 66 4.5 67 3.0 68 3.0 69 3.0 70 4.0 71 3.0 72 1.0 73 1.0 74 1.5 75 4.5 76 6.0 77 6.0 78 5.5 79 7.0 80 8.0 81 6.5 82 12.5 83 17.5 84 25.5 85 35.0 86 40.0 87 41.5 88 41.5 89 46.5 90 45.0 91 32.5 92 30.0 93 29.5 94 18.5 95 12.5 96 10.5 97 8.0 98 5.5 99 4.0 100 26.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.025 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.005 125-129 0.0 130-134 0.0 135-139 0.01 140-144 0.02 145-149 0.04 150-151 0.025 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 59.425 #Duplication Level Percentage of deduplicated Percentage of total 1 69.75178796802692 41.449999999999996 2 18.00588977702987 21.4 3 7.278081615481699 12.975 4 2.3559108119478336 5.6000000000000005 5 1.2200252419015565 3.6249999999999996 6 0.6310475389145982 2.25 7 0.12620950778291964 0.525 8 0.16827934371055953 0.8 9 0.16827934371055953 0.8999999999999999 >10 0.2103491796381994 1.6 >50 0.0 0.0 >100 0.08413967185527976 8.875 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGT 223 5.575 No Hit GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG 132 3.3000000000000003 No Hit GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGT 17 0.42500000000000004 No Hit GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGT 17 0.42500000000000004 No Hit GTCTGAGCTTTCAGGTTGTTGGACTTCCTTGAACAGTTAAGTTTTCATGT 10 0.25 No Hit TGTCGTTGTAGACTTTTCTAGAGAGAGAAATCGTGCGGTGAAGCTAAGGA 10 0.25 No Hit CGTCATCACAGCTTCAAATTAATGAAACCCAAACCCTAATACCAGGTATT 10 0.25 No Hit TCAAGCAAAAAACACTTTGCACCACAATACTAAACACACATGAAGTAGTT 9 0.22499999999999998 No Hit GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGGT 9 0.22499999999999998 No Hit GACAAATTGTCCTATCAATCACCGTCCATCTCGCCGTCAACGACCAAGAG 9 0.22499999999999998 No Hit CCTCCCATGAGATTGATGAGCAAGATAATGAAAGAGCTTTGGAAGGATTG 9 0.22499999999999998 No Hit GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGT 8 0.2 No Hit CGTCAAGGGATCTTGCTGCTCATACCACTCTAAAACCAAGAAAGGAAGGG 8 0.2 No Hit GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGGGGGGGGGGT 8 0.2 No Hit CCTCCACACGCCTTACGCGCATTCCTTAACAACATAACCGAATCCGTCCG 8 0.2 No Hit TGCAGAGTCAGCATTTCTCAAGAAATGATTTAAAATTTGTAGCTTGTGTT 7 0.17500000000000002 No Hit GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGGGGGGGGGGGGGGGT 7 0.17500000000000002 No Hit GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGGGGGGGGGGGT 7 0.17500000000000002 No Hit GGAACTGGTGGTGACTGGGGAACATTGGATGGGTATAACCAAAGTAGGGA 6 0.15 No Hit GTTGGTTTCACCTCGATGGAACAATATGATGGCTCCACAACAGCAATTTG 6 0.15 No Hit TGCAAAGGAAATTGTTGGAGAAAGATGAGCTTTTGAAATCAGCAGAAGTT 6 0.15 No Hit CCCAAAAAGCTACCACATATTTCGTTGTATTGCGAGGTATGGCTACAAGG 6 0.15 No Hit GCCATCAACACAGACCCACAGACACCCGATCACGGCACCAACACTCCCTT 6 0.15 No Hit CCCACTCCTCTAATCTCCCCTCTCTCTCTCTCTCTAAAAATAACCAAAAA 6 0.15 No Hit TTTTTATTATGAGCCAATATCTCATGTGATTAGTTTCTGGGTGGAAGAAT 6 0.15 No Hit CGGCGCACAAGGTGAGCGCAAGGAAGGGTTTGTTGACCAGATGAAGGACA 6 0.15 No Hit CTTGCTGAAGAAGTTCATAAGACTGATAATGAAGATGTTTTCATGGACAA 6 0.15 No Hit GTCTTTCCAAGTAGCAAAGGATAGCAATGACATTCTCAACTCATGGGCAA 6 0.15 No Hit GGACAAGACCACAGGACAGAAGAATAAGATCACAATTACCAATGACAAGG 6 0.15 No Hit CGTGGCGGGACACCGTGGTCTAGTCGGCTCCGCAATCGTCCGCAAGCTCC 6 0.15 No Hit CTCATCTCTTTCCTCTTCGCAAACAATGGCTCATTCTCATCCTGTAAAAC 6 0.15 No Hit CTTCTTGTAGGCTGTAACTACCGCCATGTTCCCGCTATAAAATAATTAAA 6 0.15 No Hit CAAGAAAGTAGTAAATTCGGTGTTTATCAAGTGTTTAATTTTATCTATGA 6 0.15 No Hit CTGCATGGCTTCAGCGAAAATCAAAAGGTCTATTGTTGATGGTACAACCC 5 0.125 No Hit GCAGAACGAAATTCATCCCACCAAGCAACCTCGCCCTCCCTTTCTCTTTC 5 0.125 No Hit TTATTGGCTCTCTCTTGGGTTTTATTTCTTGTTTGTTCTTGGAGAGAGAA 5 0.125 No Hit GGTGAAACTACTCGTAAAGGATTCTATTTGTATGATGATAGGCGTAAAGC 5 0.125 No Hit CTTACACCAAAAGATGATGGAAGCTTGGCCAGGCTGATCATTGAATATGA 5 0.125 No Hit GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGA 5 0.125 No Hit GCTGGATGCAGGTATTATTTTTAATGTACCATTACTTTGCTGCGACTGAA 5 0.125 No Hit CTCCTCCATCTCACAGTGCTCTCACACGCTCCAAAGCTCTCACTCTCTCA 5 0.125 No Hit TGCCAAAACAAACGAGAGCCGCCCTATTCCCACAAATCTTCCCCTTCAGC 5 0.125 No Hit GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGGGGGGGGGGGGGT 5 0.125 No Hit TCTCTCTATACAAAACCCATCGATTCAAAACACACAAAAAAACCCACCAA 5 0.125 No Hit GAGGCAACTAAGGTAGCGGTTCCAGAGTCTGTCTTGAAGAAGCAGAAGAG 5 0.125 No Hit TGTCTGCTGCAGAGAAAGCTCCATATGAAGCTAAAGCAGCAATAAAGAAA 5 0.125 No Hit CGGCGTGTGGTGAAGCGTTTTATTGATCAGAACATTGAGTTTGTCCGGAA 5 0.125 No Hit TGTCAATTTTCCTCGAAGTTCTTTGCTTTCCCTTCTTTTCTTGTTCAATT 5 0.125 No Hit TTTTGAATTCATCATCAACAATGGTGGCATTGACACTGAAGATGACTATC 5 0.125 No Hit CAAAGTATGCCACCACCTCACGTGCAATACCAACAACAATACCAACAACA 5 0.125 No Hit GGGCGGACCGCCATTGAGATGTATAGTGACTACATGAAGAGCTTCAGGGA 5 0.125 No Hit GCGGATATGAAGGGAAGATTGGAGTCAACAATGAGAGTATCTGAGGTGAT 5 0.125 No Hit GCAGAGTTGAAGAGACATAATACAATTGAACATGCTGGTCATATGTCTCG 5 0.125 No Hit GTCCAATTTGTCGGCACCCATTTAATCACTTCCCACGTGTGTGTCAATTG 5 0.125 No Hit CCTCCTCATCCTCCTCCACAACCTCATCTTCAACAACCACAACAACTCCA 5 0.125 No Hit GGGAGTTTTGCCACTGTTTCCGTCCTCGGTGCTCGCCCAATGGCGTTCAA 5 0.125 No Hit CGAAACCTAGATTGTCAATTGAACAACCAGAGCATGGATTAGGAGTCCTT 5 0.125 No Hit TTTTGCTTTTCTCTCTTGGGGCAAATACCTGGGTAAAAGATTAAGTTTTT 5 0.125 No Hit GGCGGAGAAAATTTGAGAGGAAAAGGGGCAGAACAAAATAAGCCCTAAAC 5 0.125 No Hit CTCTACTTCCTTGTAACTGTTGCAGAACTCCCACCAGAAGGAACGAAGGG 5 0.125 No Hit GTTTGGCCACATGGATTAACCTCTTCCATTTAGTGCTATTATGGGCTAAA 5 0.125 No Hit CGAGTAGTGATGATGGGTGATGATACATGGATCCAGTTGTTTCCTCATCA 5 0.125 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.05 0.0 0.0 0.0 0.0 2 0.05 0.0 0.0 0.0 0.0 3 0.05 0.0 0.0 0.0 0.0 4 0.05 0.0 0.0 0.0 0.0 5 0.05 0.0 0.0 0.0 0.0 6 0.05 0.0 0.0 0.0 0.0 7 0.05 0.0 0.0 0.0 0.0 8 0.05 0.0 0.0 0.0 0.0 9 0.05 0.0 0.0 0.0 0.0 10-11 0.05 0.0 0.0 0.0 0.0 12-13 0.05 0.0 0.0 0.0 0.0 14-15 0.05 0.0 0.0 0.0 0.0 16-17 0.05 0.0 0.0 0.0 0.0 18-19 0.05 0.0 0.0 0.0 0.0 20-21 0.05 0.0 0.0 0.0 0.0 22-23 0.05 0.0 0.0 0.0 0.0 24-25 0.05 0.0 0.0 0.0 0.0 26-27 0.05 0.0 0.0 0.0 0.0 28-29 0.05 0.0 0.0 0.0 0.0 30-31 0.05 0.0 0.0 0.0 0.0 32-33 0.05 0.0 0.0 0.0 0.0 34-35 0.05 0.0 0.0 0.0 0.0 36-37 0.05 0.0 0.0 0.0 0.0 38-39 0.0625 0.0 0.0 0.0 0.0 40-41 0.075 0.0 0.0 0.0 0.0 42-43 0.175 0.0 0.0 0.0 0.0 44-45 0.175 0.0 0.0 0.0 0.0 46-47 0.175 0.0 0.0 0.0 0.0 48-49 0.175 0.0 0.0 0.0 0.0 50-51 0.1875 0.0 0.0 0.0 0.0 52-53 0.2 0.0 0.0 0.0 0.0 54-55 0.2 0.0 0.0 0.0 0.0 56-57 0.21250000000000002 0.0 0.0 0.0 0.0 58-59 0.2625 0.0 0.0 0.0 0.0 60-61 0.3 0.0 0.0 0.0 0.0 62-63 0.5875 0.0 0.0 0.0 0.0 64-65 0.6625 0.0 0.0 0.0 0.0 66-67 0.8 0.0 0.0 0.0 0.0 68-69 0.875 0.0 0.0 0.0 0.0 70-71 1.0499999999999998 0.0 0.0 0.0 0.0 72-73 1.2 0.0 0.0 0.0 0.0 74-75 1.375 0.0 0.0 0.0 0.0 76-77 1.5375 0.0 0.0 0.0 0.0 78-79 1.875 0.0 0.0 0.0 0.0 80-81 2.2125000000000004 0.0 0.0 0.0 0.0 82-83 2.6125 0.0 0.0 0.0 0.0 84-85 3.175 0.0 0.0 0.0 0.0 86-87 3.4625 0.0 0.0 0.0 0.0 88-89 3.775 0.0 0.0 0.0 0.0 90-91 4.2 0.0 0.0 0.0 0.0 92-93 4.9 0.0 0.0 0.0 0.0 94-95 5.5 0.0 0.0 0.0 0.0 96-97 6.0 0.0 0.0 0.0 0.0 98-99 6.5375 0.0 0.0 0.0 0.0 100-101 7.25 0.0 0.0 0.0 0.0 102-103 7.6875 0.0 0.0 0.0 0.0 104-105 8.375 0.0 0.0 0.0 0.0 106-107 8.8875 0.0 0.0 0.0 0.0 108-109 9.5375 0.0 0.0 0.0 0.0 110-111 10.15 0.0 0.0 0.0 0.0 112-113 11.1375 0.0 0.0 0.0 0.0 114-115 12.375 0.0 0.0 0.0 0.0 116-117 13.275 0.0 0.0 0.0 0.0 118-119 14.05 0.0 0.0 0.0 0.0 120-121 14.9625 0.0 0.0 0.0 0.0 122-123 15.712499999999999 0.0 0.0 0.0 0.0 124-125 16.4625 0.0 0.0 0.0 0.0 126-127 17.5375 0.0 0.0 0.0 0.0 128-129 18.1875 0.0 0.0 0.0 0.0 130-131 19.275 0.0 0.0 0.0 0.0 132-133 20.2 0.0 0.0 0.0 0.0 134-135 21.15 0.0 0.0 0.0 0.0 136-137 22.35 0.0 0.0 0.0 0.0 138-139 23.3625 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGAGACA 10 0.006830828 145.0 1 ACACGAT 10 0.006830828 145.0 5 GACACGA 10 0.006830828 145.0 4 ACGATGG 10 0.006830828 145.0 7 CGATGGC 10 0.006830828 145.0 8 AGACACG 10 0.006830828 145.0 3 TTTTGGG 35 3.5374105E-6 29.0 50-54 TTTTTTT 20 0.00593511 29.0 90-94 TTTTTGG 25 4.977651E-4 29.0 50-54 GGTTTTT 20 0.00593511 29.0 45-49 GGGGGGA 35 3.5374105E-6 29.0 65-69 GGGGGTT 40 9.990927E-6 25.375 45-49 GGGTTTT 35 1.1966578E-4 24.857143 45-49 GGGGTTT 35 1.1966578E-4 24.857143 45-49 GGGGGAA 30 0.0014437955 24.166668 65-69 TTGGGGG 45 6.5511256E-4 19.333332 50-54 GGGGGGT 80 6.46479E-6 16.3125 40-44 TTTGGGG 65 4.1823133E-4 15.615384 50-54 >>END_MODULE Read 2338795 spots for SRR26075354.sra Written 2338795 spots for SRR26075354.sra Read 2338795 spots for SRR26075354.sra Written 2338795 spots for SRR26075354.sra Read 2338795 spots for SRR26075354.sra Written 2338795 spots for SRR26075354.sra Read 2338795 spots for SRR26075354.sra Written 2338795 spots for SRR26075354.sra Read 2338795 spots for SRR26075354.sra Written 2338795 spots for SRR26075354.sra Read 2338795 spots for SRR26075354.sra Written 2338795 spots for SRR26075354.sra Read 2338795 spots for SRR26075354.sra Written 2338795 spots for SRR26075354.sra Read 2338795 spots for SRR26075354.sra Written 2338795 spots for SRR26075354.sra Read 2338795 spots for SRR26075354.sra Written 2338795 spots for SRR26075354.sra Read 2338795 spots for SRR26075354.sra Written 2338795 spots for SRR26075354.sra Read 2338795 spots for SRR26075354.sra Written 2338795 spots for SRR26075354.sra Read 2338795 spots for SRR26075354.sra Written 2338795 spots for SRR26075354.sra Read 2338795 spots for SRR26075354.sra Written 2338795 spots for SRR26075354.sra Read 2338795 spots for SRR26075354.sra Written 2338795 spots for SRR26075354.sra Read 2338795 spots for SRR26075354.sra Written 2338795 spots for SRR26075354.sra Read 2338795 spots for SRR26075354.sra Written 2338795 spots for SRR26075354.sra Read 2338795 spots for SRR26075354.sra Written 2338795 spots for SRR26075354.sra Read 2338795 spots for SRR26075354.sra Written 2338795 spots for SRR26075354.sra Read 2338795 spots for SRR26075354.sra Written 2338795 spots for SRR26075354.sra Read 2338797 spots for SRR26075354.sra Written 2338797 spots for SRR26075354.sra SRR ids: ['SRR26075354.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_y34atwev SRR26075354.sra spots: 46775902 blocks: [[1, 2338795], [2338796, 4677590], [4677591, 7016385], [7016386, 9355180], [9355181, 11693975], [11693976, 14032770], [14032771, 16371565], [16371566, 18710360], [18710361, 21049155], [21049156, 23387950], [23387951, 25726745], [25726746, 28065540], [28065541, 30404335], [30404336, 32743130], [32743131, 35081925], [35081926, 37420720], [37420721, 39759515], [39759516, 42098310], [42098311, 44437105], [44437106, 46775902]] SRR26075354 file size 17277285 SRR26075354 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o SRR26075354 SRR26075354_1.fastq SRR26075354_2.fastq Input file: SRR26075354_1.fastq Paired file: SRR26075354_2.fastq trimmed: SRR26075354-trimmed-pair1.fastq, SRR26075354-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Tue Feb 11 22:02:51 2025 >> started Tue Feb 11 22:03:47 2025 >> done (55.689s) 46775902 read pairs processed; of these: 447 ( 0.00%) short read pairs filtered out after trimming by size control 5157121 (11.03%) empty read pairs filtered out after trimming by size control 41618334 (88.97%) read pairs available; of these: 13301655 (31.96%) trimmed read pairs available after processing 28316679 (68.04%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 18 18 0.00% 19 14 0.00% 20 29 0.00% 21 32 0.00% 22 54 0.00% 23 50 0.00% 24 53 0.00% 25 57 0.00% 26 69 0.00% 27 61 0.00% 28 135 0.00% 29 117 0.00% 30 122 0.00% 31 121 0.00% 32 198 0.00% 33 186 0.00% 34 195 0.00% 35 240 0.00% 36 949 0.00% 37 1167 0.00% 38 1123 0.00% 39 826 0.00% 40 1162 0.00% 41 995 0.00% 42 962 0.00% 43 1348 0.00% 44 1005 0.00% 45 1302 0.00% 46 1605 0.00% 47 1841 0.00% 48 2427 0.01% 49 2703 0.01% 50 3343 0.01% 51 3731 0.01% 52 6103 0.01% 53 3864 0.01% 54 4242 0.01% 55 4826 0.01% 56 5570 0.01% 57 6948 0.02% 58 8546 0.02% 59 10920 0.03% 60 12319 0.03% 61 13028 0.03% 62 14637 0.04% 63 17556 0.04% 64 14997 0.04% 65 15869 0.04% 66 18150 0.04% 67 20428 0.05% 68 23858 0.06% 69 27669 0.07% 70 31737 0.08% 71 36516 0.09% 72 39554 0.10% 73 41487 0.10% 74 42183 0.10% 75 42147 0.10% 76 44175 0.11% 77 47720 0.11% 78 52919 0.13% 79 59059 0.14% 80 67104 0.16% 81 73818 0.18% 82 82971 0.20% 83 82912 0.20% 84 83872 0.20% 85 83400 0.20% 86 82474 0.20% 87 86217 0.21% 88 89926 0.22% 89 97852 0.24% 90 107397 0.26% 91 115742 0.28% 92 123102 0.30% 93 126897 0.30% 94 126814 0.30% 95 124545 0.30% 96 122986 0.30% 97 123011 0.30% 98 125106 0.30% 99 131385 0.32% 100 139584 0.34% 101 148620 0.36% 102 157505 0.38% 103 160736 0.39% 104 160594 0.39% 105 159704 0.38% 106 155480 0.37% 107 151610 0.36% 108 155938 0.37% 109 158745 0.38% 110 166196 0.40% 111 177296 0.43% 112 188117 0.45% 113 193858 0.47% 114 194320 0.47% 115 193673 0.47% 116 187103 0.45% 117 185286 0.45% 118 183243 0.44% 119 181917 0.44% 120 189217 0.45% 121 201766 0.48% 122 205736 0.49% 123 215184 0.52% 124 219461 0.53% 125 218701 0.53% 126 215369 0.52% 127 210422 0.51% 128 207842 0.50% 129 207793 0.50% 130 212029 0.51% 131 221170 0.53% 132 231601 0.56% 133 239293 0.57% 134 244551 0.59% 135 243582 0.59% 136 242790 0.58% 137 234010 0.56% 138 233001 0.56% 139 232776 0.56% 140 233892 0.56% 141 238565 0.57% 142 248572 0.60% 143 250810 0.60% 144 258336 0.62% 145 261106 0.63% 146 254392 0.61% 147 250383 0.60% 148 244006 0.59% 149 240392 0.58% 150 242584 0.58% 151 28316679 68.04% 41618334 reads passed initial QC criterion=sequence-density sequence-density=0.56 sequence-density-rank=1 fanout-score=25.09 fanout-score-rank=6 prefix-density=0.56 prefix-fanout=25.1 sequence=GGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTATCGCAATCTCGG criterion=fanout-score sequence-density=0.08 sequence-density-rank=27 fanout-score=311.94 fanout-score-rank=1 prefix-density=0.91 prefix-fanout=26.2 sequence=TCTTCTTCTTCTC criterion=sequence-density sequence-density=0.37 sequence-density-rank=1 fanout-score=3.22 fanout-score-rank=24 prefix-density=0.37 prefix-fanout=3.2 sequence=CAAGGTGGGTACAACCAACAAGAGCACAGGGGCGGCGCACAAG criterion=fanout-score sequence-density=0.08 sequence-density-rank=21 fanout-score=365.86 fanout-score-rank=1 prefix-density=0.98 prefix-fanout=31.0 sequence=AAGAAGAAGAAA SRR26075354 testing PE reads STAR mapping to Ensembl genome Started job on | Feb 11 22:04:26 Started mapping on | Feb 11 22:04:27 Finished on | Feb 11 22:11:09 Mapping speed, Million of reads per hour | 372.70 Number of input reads | 41618334 Average input read length | 281 UNIQUE READS: Uniquely mapped reads number | 36001295 Uniquely mapped reads % | 86.50% Average mapped length | 278.84 Number of splices: Total | 27788462 Number of splices: Annotated (sjdb) | 27006257 Number of splices: GT/AG | 27202404 Number of splices: GC/AG | 412486 Number of splices: AT/AC | 35356 Number of splices: Non-canonical | 138216 Mismatch rate per base, % | 0.40% Deletion rate per base | 0.03% Deletion average length | 2.98 Insertion rate per base | 0.02% Insertion average length | 2.88 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 1300389 % of reads mapped to multiple loci | 3.12% Number of reads mapped to too many loci | 59506 % of reads mapped to too many loci | 0.14% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 8.83% % of reads unmapped: other | 1.40% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 4316650 4316650 4316650 N_multimapping 1300389 1300389 1300389 N_noFeature 1514739 35523291 1783099 N_ambiguous 430714 3689 218057 UnstrandedReadsAssigned:34055842 PositiveStrandReadsAssigned:474315 NegativeStrandReadsAssigned:34000139 Dataset is classified negative stranded MeadianReadLen=151 20thPercentileLength=129 echo kmer=125 SRR26075354 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,400 [index] number of k-mers: 62,057,036 [index] number of equivalence classes: 130,681 [quant] running in paired-end mode [quant] will process pair 1: SRR26075354-trimmed-pair1.fastq SRR26075354-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 41,618,334 reads, 34,714,577 reads pseudoaligned [quant] estimated average fragment length: 170.021 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,114 rounds 52401 SRR26075354.ke.tsv 34699 SRR26075354.se.tsv 87100 total ==> SRR26075354.ke.tsv <== target_id length eff_length est_counts tpm Potri.005G200100.1.v4.1 2018 1848.98 4596 82.7669 Potri.005G024800.1.v4.1 1035 865.979 1131 43.4875 Potri.004G059700.1.v4.1 961 791.979 49 2.06011 Potri.007G009000.2.v4.1 1416 1246.98 0 0 Potri.003G141000.2.v4.1 2943 2773.98 1445 17.345 Potri.016G087400.1.v4.1 270 110.935 2200 660.335 Potri.015G069301.1.v4.1 564 395.079 0 0 Potri.010G195200.1.v4.1 1773 1603.98 336 6.97508 Potri.012G127500.1.v4.1 977 807.979 27701 1141.57 ==> SRR26075354.se.tsv <== Potri.001G166300.v4.1 0 Potri.001G448400.v4.1 498 Potri.001G233950.v4.1 0 Potri.001G122700.v4.1 592 Potri.001G212900.v4.1 26 Potri.001G182400.v4.1 2 Potri.001G256600.v4.1 0 Potri.001G040500.v4.1 0 Potri.001G416900.v4.1 0 Potri.001G452600.v4.1 1426 SRR26075354 completed mapping pipeline successfully