Starting /dee2/code/volunteer_pipeline.sh SRR26075355 current disk space = 3052591513600 free memory = 1400077700 SRR26075355 SRAfilesize b710476943eabfac20e8955a3bed31fd SRR26075355.sra SRR26075355.sra file validated SRR26075355 is paired end SRR26075355 is conventional basespace SRR26075355 read1 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR26075355_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.57975 37.0 37.0 37.0 37.0 37.0 2 36.657 37.0 37.0 37.0 37.0 37.0 3 36.649 37.0 37.0 37.0 37.0 37.0 4 36.76 37.0 37.0 37.0 37.0 37.0 5 36.759 37.0 37.0 37.0 37.0 37.0 6 36.7555 37.0 37.0 37.0 37.0 37.0 7 36.6645 37.0 37.0 37.0 37.0 37.0 8 36.6095 37.0 37.0 37.0 37.0 37.0 9 36.674 37.0 37.0 37.0 37.0 37.0 10-14 36.661500000000004 37.0 37.0 37.0 37.0 37.0 15-19 36.5911 37.0 37.0 37.0 37.0 37.0 20-24 36.5544 37.0 37.0 37.0 37.0 37.0 25-29 36.4634 37.0 37.0 37.0 37.0 37.0 30-34 36.4433 37.0 37.0 37.0 37.0 37.0 35-39 36.375099999999996 37.0 37.0 37.0 37.0 37.0 40-44 36.2957 37.0 37.0 37.0 37.0 37.0 45-49 36.133799999999994 37.0 37.0 37.0 37.0 37.0 50-54 36.131 37.0 37.0 37.0 37.0 37.0 55-59 36.0537 37.0 37.0 37.0 37.0 37.0 60-64 35.99250000000001 37.0 37.0 37.0 37.0 37.0 65-69 35.950500000000005 37.0 37.0 37.0 37.0 37.0 70-74 35.96319999999999 37.0 37.0 37.0 37.0 37.0 75-79 36.0 37.0 37.0 37.0 37.0 37.0 80-84 36.0098 37.0 37.0 37.0 37.0 37.0 85-89 35.9253 37.0 37.0 37.0 37.0 37.0 90-94 35.825300000000006 37.0 37.0 37.0 37.0 37.0 95-99 35.7637 37.0 37.0 37.0 37.0 37.0 100-104 35.7219 37.0 37.0 37.0 37.0 37.0 105-109 35.615700000000004 37.0 37.0 37.0 37.0 37.0 110-114 35.4993 37.0 37.0 37.0 37.0 37.0 115-119 35.4146 37.0 37.0 37.0 34.6 37.0 120-124 35.493700000000004 37.0 37.0 37.0 37.0 37.0 125-129 35.2401 37.0 37.0 37.0 34.6 37.0 130-134 35.1259 37.0 37.0 37.0 25.0 37.0 135-139 34.978899999999996 37.0 37.0 37.0 25.0 37.0 140-144 34.8332 37.0 37.0 37.0 25.0 37.0 145-149 34.668299999999995 37.0 37.0 37.0 25.0 37.0 150-151 34.41775 37.0 37.0 37.0 25.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.0 1101 2 0.0 1101 3 0.0 1101 4 0.0 1101 5 0.0 1101 6 0.0 1101 7 0.0 1101 8 0.0 1101 9 0.0 1101 10-14 0.0 1101 15-19 0.0 1101 20-24 0.0 1101 25-29 0.0 1101 30-34 0.0 1101 35-39 0.0 1101 40-44 0.0 1101 45-49 0.0 1101 50-54 0.0 1101 55-59 0.0 1101 60-64 0.0 1101 65-69 0.0 1101 70-74 0.0 1101 75-79 0.0 1101 80-84 0.0 1101 85-89 0.0 1101 90-94 0.0 1101 95-99 0.0 1101 100-104 0.0 1101 105-109 0.0 1101 110-114 0.0 1101 115-119 0.0 1101 120-124 0.0 1101 125-129 0.0 1101 130-134 0.0 1101 135-139 0.0 1101 140-144 0.0 1101 145-149 0.0 1101 150-151 0.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 17 1.0 18 0.0 19 1.0 20 1.0 21 1.0 22 7.0 23 6.0 24 15.0 25 6.0 26 7.0 27 16.0 28 21.0 29 29.0 30 33.0 31 48.0 32 70.0 33 142.0 34 151.0 35 466.0 36 2784.0 37 195.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 35.73039338511651 13.329992483086944 8.64445001252819 42.29516411926835 2 16.675 13.775 36.9 32.65 3 16.0 15.8 29.95 38.25 4 22.225 22.625 23.375 31.775 5 24.2 26.700000000000003 26.85 22.25 6 23.200000000000003 30.875000000000004 23.575 22.35 7 15.275 26.6 39.375 18.75 8 17.525 26.900000000000002 32.45 23.125 9 18.85 22.3 35.625 23.225 10-14 19.401940194019403 28.562856285628563 27.752775277527753 24.282428242824285 15-19 20.365 26.424999999999997 28.449999999999996 24.759999999999998 20-24 20.355 26.395000000000003 28.96 24.29 25-29 19.220000000000002 26.745 28.175 25.86 30-34 20.885 25.81 28.27 25.035 35-39 20.150000000000002 27.325 27.689999999999998 24.834999999999997 40-44 20.28 27.250000000000004 27.63 24.84 45-49 20.435 26.47 28.035 25.06 50-54 21.005 25.7 27.575 25.72 55-59 20.685000000000002 25.955000000000002 28.32 25.040000000000003 60-64 20.325 25.89 27.765 26.02 65-69 20.080000000000002 27.295 28.16 24.465 70-74 21.345 26.455000000000002 27.005000000000003 25.195 75-79 21.78 26.13 27.665 24.425 80-84 21.92 26.150000000000002 27.084999999999997 24.845 85-89 21.625 27.389999999999997 26.490000000000002 24.495 90-94 21.44 26.215 27.834999999999997 24.51 95-99 21.285 27.01 27.425 24.279999999999998 100-104 21.27 25.985000000000003 27.22 25.525 105-109 22.53 25.785000000000004 26.640000000000004 25.045 110-114 21.05 26.735 28.095 24.12 115-119 21.97 26.395000000000003 26.939999999999998 24.695 120-124 21.72 26.900000000000002 26.479999999999997 24.9 125-129 21.59 26.105 27.61 24.695 130-134 22.375 27.375 26.179999999999996 24.07 135-139 22.165000000000003 27.375 25.509999999999998 24.95 140-144 22.38 26.279999999999998 25.755 25.585 145-149 23.255 27.01 25.185000000000002 24.55 150-151 24.0125 26.8375 24.4875 24.6625 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.5 15 1.5 16 1.5 17 0.5 18 0.0 19 0.0 20 0.5 21 0.5 22 2.5 23 4.0 24 2.5 25 1.0 26 0.5 27 2.0 28 3.0 29 4.5 30 8.0 31 13.5 32 22.5 33 29.0 34 29.0 35 37.0 36 55.5 37 71.5 38 89.5 39 124.0 40 166.0 41 174.5 42 178.5 43 225.0 44 249.0 45 250.0 46 285.0 47 307.0 48 280.0 49 241.5 50 217.5 51 176.0 52 143.5 53 126.5 54 106.0 55 83.5 56 56.0 57 40.5 58 33.5 59 27.0 60 24.0 61 18.5 62 7.5 63 9.5 64 8.0 65 4.0 66 5.5 67 12.5 68 11.5 69 7.5 70 8.5 71 4.5 72 2.5 73 2.0 74 1.5 75 1.0 76 0.5 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.22499999999999998 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.01 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150-151 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 59.199999999999996 #Duplication Level Percentage of deduplicated Percentage of total 1 62.204391891891895 36.825 2 21.91722972972973 25.95 3 8.488175675675675 15.075 4 3.927364864864865 9.3 5 1.6469594594594592 4.875 6 0.8023648648648648 2.85 7 0.46452702702702703 1.925 8 0.16891891891891891 0.8 9 0.21114864864864866 1.125 >10 0.16891891891891891 1.275 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CCCTCCCCTAACTTGCGCATGCTAATAACTTGAAGAGATACAAAACAGGC 18 0.44999999999999996 No Hit GGGGAGACAACAAGGGAGAATCCAACAATGGCACCAGAGACTGGCAAAGA 13 0.325 No Hit TGTCATCTAAGCCTTGTTTGCGATGTTGTTTGAGAGCCAGTCCAATCCCT 10 0.25 No Hit CACGGCTGTAACCACCGCCGCCTTCACGGCGTCCACCTCCTCCATAACCG 10 0.25 No Hit CTCGAATAAAAAAAACGTAACATAGCATACCATCAGCATCACGAGGCGGG 9 0.22499999999999998 No Hit CTCTCACCTTCACTAGCCATGTCAACATTCTCCAAAGTTCCATTAAACTC 9 0.22499999999999998 No Hit GTCCTGACTTGAAAAGAGCCATCACCAAGCAACCAAAGCAGCACCACCGA 9 0.22499999999999998 No Hit GGCGGATCTGAAGCCCATTTGGAGATTGTAACTGGGTTTGTGTGCTTGCT 9 0.22499999999999998 No Hit CCTTCTTATATCCATGAAAGTCTCCTTCTTGTTTTCCCGCTTCTTGTCTT 9 0.22499999999999998 No Hit ATACAAGGAATCACGATTGATGGGTTCAACATGCCAATTTCGCTCGTTGC 8 0.2 No Hit TTTCTCTGGGAAGGTTATTTTTGTTCCCCTTTTCCAACCAGGCTTGATAT 8 0.2 No Hit CCTGGCTAGTTATCACACTTAATAAAAGACTACAAGAGCTGACAATAAGA 8 0.2 No Hit CCACTCCACAACCCACATATATCCATCGATCCCTTCTTTTTTTCTTTTTC 8 0.2 No Hit CATGGAGATAGACATGTGTACGTGCTCCCAGTTTGAAAGGTTATCTCCCG 7 0.17500000000000002 No Hit CTTCTTTCACCTTCTTGAACCCAATTGAAGCAACCTGTTTGGTCTTTCCA 7 0.17500000000000002 No Hit GGTGGGGGATGGTGCATTTGCTGCCGGTGGTGGGGTTTCCAATGGAGATG 7 0.17500000000000002 No Hit CACATCAACTTCTGGTTCATCACTCACAACAACTTCATTAGGTTCCCCGG 7 0.17500000000000002 No Hit CTTGGAGAGACTTAGAAGAGATAGTAGTCCATGATCATACGGCTTGCACA 7 0.17500000000000002 No Hit CCTATATATCTCAGAAAGAATTGTTTGAAAAGCCTTGTCAACATTGGTGG 7 0.17500000000000002 No Hit CTCCTTAATCTCCTTCTCAATCTTCTGCTTGTCGGCCGGGTCCAACTTAC 7 0.17500000000000002 No Hit CTGCGGGACTAGTGGTAGTAGTGACTTCCTGGCTTTCGGATGTTGATGAT 7 0.17500000000000002 No Hit CCAGAGTATTCTCATCTTCAACAGTAACCTGGATATCAGACTTGGACAAG 7 0.17500000000000002 No Hit GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTGCAGTATCTCGTTT 7 0.17500000000000002 TruSeq Adapter, Index 14 (97% over 38bp) CTTCAACTTGAATAAAATTTTGCACATTAATACAATTTGGCCAGCAAATG 7 0.17500000000000002 No Hit TCTGGATATCAAACAGCATTCTGTCATCATCACTTGTCACAAAGTTAATA 6 0.15 No Hit CCTAAAACAGAATCAGGAACAGTGCAGCCACGGGTGAACAGAGTCGGAGG 6 0.15 No Hit CTCATATTCAACCTTCCTCTTGTCCGCTTTGGCAACAAAGGGTGCTTTCT 6 0.15 No Hit GTTATGTAATACATTGAGCACTTTTGTGCATGTTAACAGGGAGACCGGAA 6 0.15 No Hit CCTTCACAGTGTTCCTCATGTTGTAGGCGTAGTTCTCCAAAGAATTCTTG 6 0.15 No Hit ACTCCTCTTCCAATCCGATCTAACCCAGCGGCTCTCCCAACCATCTTCAA 6 0.15 No Hit GTTCCATTCAGTGGCTTGTTGCTTTTGTCATAGGAATTTGCAAGTACAGA 6 0.15 No Hit GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTGCAGTATCGCGGTT 6 0.15 TruSeq Adapter, Index 14 (97% over 38bp) GACTGCTTTCTTCTCGTCATCTCCAGCAGCTACGAACTCTTTTACCAAGG 6 0.15 No Hit CCAAGAAACATCAGGCTCATCAACATGTGAAGCTGTCAAGCTGGCAGCTG 6 0.15 No Hit GGGAAAGCATTTCAGACATATATTTGGCTGTGTTTATGAAAGCAGGGTCC 6 0.15 No Hit CCAAGCTCCAAATTAGGTTAGATAAAACATCTCAACATGGGACGAGCGAC 6 0.15 No Hit CCAGTGATCGAGATACGCGGATCCCAGCAACCTGCAAAAATTCCATTGTT 6 0.15 No Hit TCTGCCTTTCCATGTGTTTCGCAGGTGGTGGTGGAATTACAACAGGTGCA 6 0.15 No Hit CTTAGAAATATATCAGCCAGTGCTCCCTGAATAGCAGCTCGATAGAAAAG 6 0.15 No Hit GGATAGTGGTGTTGCGGGGGATGAGCTTGGTCATCATACCGCCAGCAGTC 6 0.15 No Hit CTCCAGGACCTAAAACCAGGACATGATGAGCAGTTACAGGCGAAGAAGCT 6 0.15 No Hit GTCGTGCTTCCAAAGCAAATATAAACAACTGAATTTGGTTTCTTTGAGTC 6 0.15 No Hit AGCACATGCCGAACATAATGGTGATAGGGAAAGCATTAACATTGTACAGA 6 0.15 No Hit CTCTCGCCACATGCAGCCCTGAAAGCCATGCCACAAACCCTTCTTCTGCT 5 0.125 No Hit CTCTGCTCAACATCTCATCAGATTCATCAAGAACTAGTACTCTGATGGCT 5 0.125 No Hit GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTGCAGTATCGCGTTT 5 0.125 TruSeq Adapter, Index 14 (97% over 38bp) CTCTTGTAGAACCCAATACGATCCTCCACCTGTTGCTTCCATCCCATACC 5 0.125 No Hit CCTGTCATCTTCAATCTCGACTTTCACTTCCTCCTTTTTAAGCCCCGGGA 5 0.125 No Hit ACCGATAGCCCAAACTTAGTATCTTCTTCGTCGTCATCATCGTCATCAAC 5 0.125 No Hit GTCACGTTTCAAAAGATCGATTTCTCCATCTAATCCAAAACGGCTAACTT 5 0.125 No Hit TTCAAAATTAACACAACTAGATCTCAGCAGAGAGTGAGTTTACGGTCCAG 5 0.125 No Hit GCCCCTTTGACCTCCGATACAATCCCCTGCAAACCACCCTCCAAGCACAC 5 0.125 No Hit GCTAAATAGTCCGTCACACGGAGGAACATTGCACAGCGGCAATCATCAGC 5 0.125 No Hit ACCTCCTCTTTGCAGTATTGGTCGCAGGTTTCATCCCATTCTTCTGGAGA 5 0.125 No Hit GCTGTTATTCCCACTAACCAATTGTGTCCATGAGGATTTATTGAGCCATG 5 0.125 No Hit GTTGCCCGGGAAGTTGGCGCACGCGCTCGGTCGTGGTGCCATTCTTATCC 5 0.125 No Hit CTTCTCTTCCAAAGCTATCCTCACCCTTGCTGCAAAGGGACTCATCTCAA 5 0.125 No Hit GGATCTTCTACCACTTTAGTCAACAAACAAGCTTGGCAATGGCAAGACTC 5 0.125 No Hit TGTTATTCGCTCTTCAAAATTCTTGGTTGTCTTATGGGGATTGAGGTAAA 5 0.125 No Hit ATGCAAGTTCAACAGCCTCCTACTGAAACAGAATCCTATCATGCCCTTTT 5 0.125 No Hit CATTTGGTTCCAGTCCCAACTTGCATATGGTTCTGCTTGGTATCCTCCAC 5 0.125 No Hit ACGGGAACTGTTGGATTGACTTCCTTTATGATCATCACCGGGCAAAGATG 5 0.125 No Hit GGCAGTTAAAGTTTTAGAAAATAATGGATGCAGGGATATTATTTTATTGC 5 0.125 No Hit GCCACATCTACCTTGATCTGCCCTGCGCCTGCCTTGAGCGCATCAATCGC 5 0.125 No Hit ACCCCCTTTGCCTTTTCTTCAAGCTCCTCCTCCAATTGCTTTAGATGTTC 5 0.125 No Hit GTATGAAGTCGGGAAAAAAAAAGAGGGAGAAAGGAGAATATTACCTCTCT 5 0.125 No Hit CTCAACTGGTCACATATGTAAGCCCTCTCCAGTTGCTCAACCCTCTGCGA 5 0.125 No Hit ATCAATGTTCGATCCTTTCCTTGGAATTATGGCACAATTTATCTCTTCCC 5 0.125 No Hit GCCTTAAAGACATGGGCTTCTGGGGTCTCTTTCCAATCGATGCGCGTGTT 5 0.125 No Hit ATTCCTTTTCCTTGGTGAAGGAGATCATCTCAACTTCATAGTACACTGTT 5 0.125 No Hit TCCCGATCTTTTCCCTATCTAATTCACCTCCTATTAGGAGCCGATCGTGC 5 0.125 No Hit GGTTTCTGTGATGTGTTTTCCCAATGACTTTCATCTCATTCTGAAATTCT 5 0.125 No Hit CCGTCTTGGCACTCCCTAAATTGACGATCTTGGGACATCCATCCCTATCT 5 0.125 No Hit ATCTTGGATCGAATAATATCAGGTAAGCAGAGTCTAAATAGCTGCATGGG 5 0.125 No Hit ACAGAGTACACGCATCATTAAGATGGATTGTTGAGCATTGTTCTTCCTAG 5 0.125 No Hit CTTGTGGACTAGGATCAGTCACAGCCCCAGCTGATGCACCAGAAGCTAAA 5 0.125 No Hit TTCTTCTTGTAAGGCTTGTTTTTGCCTTCACGAGAAGCATCAAGTTCATC 5 0.125 No Hit GCCAGATCAGTCTCGTCGTAATCCTTCTTCTCAGACTTTGGCGCCTTCAA 5 0.125 No Hit GTCCTGCTGGTGTTGGATTTTTGGCTGGAAAACATTGTGACCTTCTGTGA 5 0.125 No Hit CCCTAGCTGATTAGGATACTTCTGCAAGCCACTCTTTTCCAAATATTTGG 5 0.125 No Hit CCTTATCATTTGTGAAGGCCTGGTAGTTTAGAACTTCCCCGGACTTCGTT 5 0.125 No Hit GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTGCAGTATCTCGGTT 5 0.125 TruSeq Adapter, Index 14 (97% over 38bp) >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0125 0.0 0.0 0.0 0.0 60-61 0.025 0.0 0.0 0.0 0.0 62-63 0.025 0.0 0.0 0.0 0.0 64-65 0.025 0.0 0.0 0.0 0.0 66-67 0.025 0.0 0.0 0.0 0.0 68-69 0.025 0.0 0.0 0.0 0.0 70-71 0.037500000000000006 0.0 0.0 0.0 0.0 72-73 0.05 0.0 0.0 0.0 0.0 74-75 0.05 0.0 0.0 0.0 0.0 76-77 0.07500000000000001 0.0 0.0 0.0 0.0 78-79 0.1 0.0 0.0 0.0 0.0 80-81 0.225 0.0 0.0 0.0 0.0 82-83 0.2875 0.0 0.0 0.0 0.0 84-85 0.4125 0.0 0.0 0.0 0.0 86-87 0.55 0.0 0.0 0.0 0.0 88-89 0.7125 0.0 0.0 0.0 0.0 90-91 1.1 0.0 0.0 0.0 0.0 92-93 1.35 0.0 0.0 0.0 0.0 94-95 1.5625 0.0 0.0 0.0 0.0 96-97 2.05 0.0 0.0 0.0 0.0 98-99 2.375 0.0 0.0 0.0 0.0 100-101 2.75 0.0 0.0 0.0 0.0 102-103 3.15 0.0 0.0 0.0 0.0 104-105 3.575 0.0 0.0 0.0 0.0 106-107 4.1875 0.0 0.0 0.0 0.0 108-109 4.5 0.0 0.0 0.0 0.0 110-111 5.0125 0.0 0.0 0.0 0.0 112-113 5.825 0.0 0.0 0.0 0.0 114-115 6.375 0.0 0.0 0.0 0.0 116-117 7.800000000000001 0.0 0.0 0.0 0.0 118-119 8.725000000000001 0.0 0.0 0.0 0.0 120-121 9.5625 0.0 0.0 0.0 0.0 122-123 10.6625 0.0 0.0 0.0 0.0 124-125 11.712499999999999 0.0 0.0 0.0 0.0 126-127 12.675 0.0 0.0 0.0 0.0 128-129 13.600000000000001 0.0 0.0 0.0 0.0 130-131 14.6875 0.0 0.0 0.0 0.0 132-133 15.725 0.0 0.0 0.0 0.0 134-135 16.862499999999997 0.0 0.0 0.0 0.0 136-137 17.85 0.0 0.0 0.0 0.0 138-139 18.987499999999997 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CCCCTAA 10 0.006830828 145.0 5 CCCTCCC 10 0.006830828 145.0 1 TCCCCTA 10 0.006830828 145.0 4 AATTGAG 10 0.006830828 145.0 145 GATCGGA 65 4.1823133E-4 15.615384 135-139 GAAGAGC 75 0.0012377208 13.533334 140-144 AGATCGG 65 0.0076375785 13.384615 135-139 >>END_MODULE SRR26075355 read2 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR26075355_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.2505 37.0 37.0 37.0 37.0 37.0 2 36.259 37.0 37.0 37.0 37.0 37.0 3 36.2525 37.0 37.0 37.0 37.0 37.0 4 36.2995 37.0 37.0 37.0 37.0 37.0 5 36.305 37.0 37.0 37.0 37.0 37.0 6 36.1435 37.0 37.0 37.0 37.0 37.0 7 36.1935 37.0 37.0 37.0 37.0 37.0 8 36.2535 37.0 37.0 37.0 37.0 37.0 9 36.312 37.0 37.0 37.0 37.0 37.0 10-14 36.1683 37.0 37.0 37.0 37.0 37.0 15-19 36.1293 37.0 37.0 37.0 37.0 37.0 20-24 36.0632 37.0 37.0 37.0 37.0 37.0 25-29 35.930099999999996 37.0 37.0 37.0 37.0 37.0 30-34 35.819 37.0 37.0 37.0 37.0 37.0 35-39 35.6948 37.0 37.0 37.0 37.0 37.0 40-44 35.6726 37.0 37.0 37.0 37.0 37.0 45-49 35.5544 37.0 37.0 37.0 37.0 37.0 50-54 35.512 37.0 37.0 37.0 37.0 37.0 55-59 35.4878 37.0 37.0 37.0 37.0 37.0 60-64 35.614599999999996 37.0 37.0 37.0 37.0 37.0 65-69 35.5145 37.0 37.0 37.0 37.0 37.0 70-74 35.4375 37.0 37.0 37.0 37.0 37.0 75-79 35.350300000000004 37.0 37.0 37.0 37.0 37.0 80-84 35.45020000000001 37.0 37.0 37.0 37.0 37.0 85-89 35.404 37.0 37.0 37.0 37.0 37.0 90-94 35.3335 37.0 37.0 37.0 37.0 37.0 95-99 35.452 37.0 37.0 37.0 37.0 37.0 100-104 35.3428 37.0 37.0 37.0 37.0 37.0 105-109 35.2676 37.0 37.0 37.0 34.6 37.0 110-114 35.3255 37.0 37.0 37.0 37.0 37.0 115-119 35.2044 37.0 37.0 37.0 32.2 37.0 120-124 35.0533 37.0 37.0 37.0 27.4 37.0 125-129 35.030100000000004 37.0 37.0 37.0 25.0 37.0 130-134 34.989 37.0 37.0 37.0 25.0 37.0 135-139 34.8133 37.0 37.0 37.0 25.0 37.0 140-144 34.7915 37.0 37.0 37.0 25.0 37.0 145-149 34.859500000000004 37.0 37.0 37.0 25.0 37.0 150-151 34.498999999999995 37.0 37.0 37.0 25.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.0 1101 2 0.0 1101 3 0.0 1101 4 0.0 1101 5 0.0 1101 6 0.0 1101 7 0.0 1101 8 0.0 1101 9 0.0 1101 10-14 0.0 1101 15-19 0.0 1101 20-24 0.0 1101 25-29 0.0 1101 30-34 0.0 1101 35-39 0.0 1101 40-44 0.0 1101 45-49 0.0 1101 50-54 0.0 1101 55-59 0.0 1101 60-64 0.0 1101 65-69 0.0 1101 70-74 0.0 1101 75-79 0.0 1101 80-84 0.0 1101 85-89 0.0 1101 90-94 0.0 1101 95-99 0.0 1101 100-104 0.0 1101 105-109 0.0 1101 110-114 0.0 1101 115-119 0.0 1101 120-124 0.0 1101 125-129 0.0 1101 130-134 0.0 1101 135-139 0.0 1101 140-144 0.0 1101 145-149 0.0 1101 150-151 0.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 13 2.0 14 8.0 15 13.0 16 13.0 17 11.0 18 3.0 19 7.0 20 8.0 21 12.0 22 6.0 23 16.0 24 11.0 25 16.0 26 22.0 27 24.0 28 15.0 29 21.0 30 19.0 31 34.0 32 44.0 33 75.0 34 180.0 35 641.0 36 2593.0 37 206.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.475 21.925 11.899999999999999 22.7 2 31.05 24.975 27.3 16.675 3 25.174999999999997 27.125 29.025000000000002 18.675 4 27.224999999999998 32.85 19.875 20.05 5 29.849999999999998 35.5 19.625 15.024999999999999 6 24.975 39.125 19.075 16.825000000000003 7 25.35 22.650000000000002 34.599999999999994 17.4 8 25.85 25.825 23.25 25.074999999999996 9 25.900000000000002 25.825 26.974999999999998 21.3 10-14 26.955000000000002 28.22 24.505 20.32 15-19 25.8 28.044999999999998 25.72 20.435 20-24 26.215 28.360000000000003 25.535000000000004 19.89 25-29 26.825 27.439999999999998 24.92 20.815 30-34 26.595000000000002 29.01 24.695 19.7 35-39 26.534999999999997 28.655 25.324999999999996 19.485 40-44 25.695 27.639999999999997 25.485000000000003 21.18 45-49 26.13 28.535 24.895 20.44 50-54 25.040000000000003 27.41 27.175 20.375 55-59 25.915 27.98 25.655 20.45 60-64 26.705000000000002 27.200000000000003 25.724999999999998 20.369999999999997 65-69 25.679999999999996 27.650000000000002 25.52 21.15 70-74 25.55 28.65 25.88 19.919999999999998 75-79 25.25 29.095 26.179999999999996 19.475 80-84 25.11 28.84 25.759999999999998 20.29 85-89 25.825 28.62 25.295 20.26 90-94 26.035000000000004 28.999999999999996 25.765 19.2 95-99 26.0 28.83 25.44 19.73 100-104 26.93 29.099999999999998 24.25 19.72 105-109 25.4 29.409999999999997 25.35 19.84 110-114 26.87 28.27 25.3 19.56 115-119 27.32 28.744999999999997 24.27 19.665 120-124 28.139999999999997 28.68 24.465 18.715 125-129 28.33 28.634999999999998 24.44 18.595 130-134 29.18 27.76 24.72 18.34 135-139 29.04 27.839999999999996 24.465 18.655 140-144 30.04 28.78 24.025 17.155 145-149 29.04 28.485 24.46 18.015 150-151 29.725 28.3875 25.424999999999997 16.4625 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.5 3 1.5 4 1.5 5 0.5 6 0.5 7 0.5 8 0.0 9 1.0 10 1.0 11 0.0 12 0.0 13 1.0 14 2.0 15 1.5 16 1.0 17 0.5 18 0.5 19 1.0 20 1.0 21 0.5 22 0.5 23 1.5 24 3.0 25 3.0 26 3.0 27 3.5 28 5.0 29 7.5 30 10.0 31 13.5 32 12.5 33 17.0 34 25.5 35 48.0 36 51.0 37 48.0 38 73.5 39 122.5 40 151.5 41 163.0 42 208.0 43 243.0 44 271.0 45 269.5 46 274.5 47 315.0 48 298.0 49 244.0 50 204.5 51 155.5 52 118.0 53 104.0 54 90.5 55 74.0 56 59.5 57 57.5 58 43.5 59 26.5 60 21.5 61 13.5 62 9.0 63 12.5 64 15.5 65 12.5 66 7.0 67 1.0 68 0.5 69 2.0 70 2.0 71 1.5 72 1.5 73 1.5 74 1.0 75 1.0 76 1.0 77 0.5 78 2.0 79 2.5 80 1.5 81 1.0 82 2.0 83 7.0 84 6.5 85 2.0 86 2.5 87 4.0 88 2.5 89 2.0 90 2.0 91 0.5 92 0.5 93 0.5 94 1.5 95 1.0 96 0.5 97 2.0 98 2.0 99 1.0 100 6.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150-151 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 60.9 #Duplication Level Percentage of deduplicated Percentage of total 1 65.10673234811166 39.65 2 19.991789819376027 24.349999999999998 3 8.292282430213465 15.15 4 3.6124794745484397 8.799999999999999 5 1.354679802955665 4.125 6 0.6157635467980296 2.25 7 0.41050903119868637 1.7500000000000002 8 0.16420361247947454 0.8 9 0.20525451559934318 1.125 >10 0.24630541871921183 2.0 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG 20 0.5 No Hit AGGAGGGTTTTGGGTATTTGGATTTGATCAGATCTCAATTCGGGTTCTGT 17 0.42500000000000004 No Hit AAAGCCAAGCTCCCAGACCTAAGCTTTACAAAGAAAACACATCAATCTCT 13 0.325 No Hit GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGT 10 0.25 No Hit CGTGAACGAAGCTCAATCCCGCGGAAGTGGTGGAGGTGGTGGTGGCGGCG 10 0.25 No Hit GTTGAGGGATGCTGTGCTGCTAGTGTTTGCAAACAAACAGGATCTTCCGA 10 0.25 No Hit GTGGTGATGTAAATGATGATGATGATGCACTAGAGATGGCAATAGAGCAT 9 0.22499999999999998 No Hit GCTAAAGACAGCTTTCTGTGCTAGAGATTTGGATTTGGAGATGGAATTGC 9 0.22499999999999998 No Hit GACAGCACCCGCAGAAAGGGTGGCAGAAGGGGAAGAAGGCTGTAAGCTGT 9 0.22499999999999998 No Hit GCAGATTCGAATCTGCAGACATTTCCTCCATCAGGTTCGCAGGGGAAGAT 9 0.22499999999999998 No Hit GTTGATTCTATCGCTGCTGCTGCTAATGGTGATGATTCTGTTGTGTCTGC 9 0.22499999999999998 No Hit AATCAATACAGCTCCTCGGAAAGCTCCTCCAATAGAGAATACATTGCCTT 8 0.2 No Hit GGTCAACAAGCGTAGATTTTCTATGTTTTTGTAGATTAAGCCCATGCTAA 8 0.2 No Hit GACATATATTAGCGTTGGCCATAGGCGAACTCTCTATGACCACCATAACA 8 0.2 No Hit GATTTCCTGGGTTCCAGATCCTCGTACTGGATTCTACAGACCAGAGAATG 8 0.2 No Hit GAGACATCATGGGCTGATCAATGGGACTACAACAAAGATCCTGTTGTCTA 7 0.17500000000000002 No Hit TCATGTTGCAGTCAAATGGTTGATGTTACTCCAAAGTCCAACCTTGAGAA 7 0.17500000000000002 No Hit GTGAAGAATAAGATTACCATCACCAATGACAAGGGAAGACTGGGCAAGGA 7 0.17500000000000002 No Hit GAATGAGTGTAATGCTGCTGTTGGGTTCAAATGGATGCTTAATCAGGGCG 7 0.17500000000000002 No Hit GTCCTCGAGGGTGAACTGCCAGGTATCGATCAGAAGGATGTCACGATCGA 7 0.17500000000000002 No Hit ATCCGCGGTCGAGTGATCATACAACCAATGAAATCAGGAGCAATAGTATT 7 0.17500000000000002 No Hit CAGCAACTGAACCATGATTACCTTTACGGGTTTGAGCTTTCATTTGACAG 7 0.17500000000000002 No Hit GATGCAGCACTGCTTGTCAGAAAACTGCAGAGCACCTTCCCTGTTTACTT 7 0.17500000000000002 No Hit GCACAATATTCTCCTTCATCTTCCCCTGCATCCTCACCCACCAAATCACC 7 0.17500000000000002 No Hit GTTGATTACATGAATCAGGATTTACCAACTATTGAGAACGAGATGGAGAC 7 0.17500000000000002 No Hit CTCAGCCGCCTTTCTTACCCGCTGTCCCAGTTTTGAAGAGATCCAATAGC 6 0.15 No Hit CGTTAATTGGGAACAAGACTGATCTTAAACATCTTAGAGCAGTAGCTACT 6 0.15 No Hit TGGAGGAGTTCAGAGAGCAGTACAAGAGGGAACACCCTAAAAACAAATCC 6 0.15 No Hit TTTACATCTAATGGTGTTGATGGCATATGGAATGATATGAATGAGCCAGC 6 0.15 No Hit TGGGGTTGAAGAGAATTATAGCAGGGAAATCAATGGGAGTGCAGTCTCCA 6 0.15 No Hit GTTAGGGCAGCGTCTGAGGAAGTTTGCTGCGGTTTCGCCTTGACCGCGGG 6 0.15 No Hit AAATTATGATCTGCCGACTCAACCTGAAAACTATCTTCACCGTATTGGAC 6 0.15 No Hit GGAAAATCTTGGTCTTCATCGCTTGATTGAAGCGATGAAGCACATGTTTG 6 0.15 No Hit GCGGAACGAATACACATCAGCATTCAGCAGAGAAAGACAGCTGCATATGC 6 0.15 No Hit GAAGTCGTGAAGTTGTCGCCTCATGCTCGTGCCATTAGAGATGTGGAGGA 6 0.15 No Hit GCTTGTGTAGGGATATTCTGGCCTTCCATTATGAAGATGAGGTCCCAATA 6 0.15 No Hit AGCAAATCATTTTAATCATGAGGAATCAGAGGAAGAGGAGGAGCCTGAAC 6 0.15 No Hit GCAGCATTTCCGCAATGACGGACTTTGCATTAGCTATCAGTACTAAAGTT 6 0.15 No Hit GGGAAATTGGACTGGGGCATTCAAGGAGATGAGCTGAACAAGCTCAAGAA 6 0.15 No Hit GGGTTTCTTACTGTCACTGTGCCTAAGGAGGAAGTCAAGAAACCTGAAGT 6 0.15 No Hit TGAAAATACAAGGGAGCACATGCTTGATATTTCATTCTATAAACCATCAT 5 0.125 No Hit TTTCAATTGATGTTGATGTTTCAAGTAGTAGCAGCAGCAGCACCGATCGC 5 0.125 No Hit GGTGGTTGGTTGGAGAGGACTACTGTCGAGGACATGGACATTGCTGTTCG 5 0.125 No Hit ACGGGAACTGATGCCTATGCCTTGGCCAAGAAAGCTGGGCGTTATAAGCA 5 0.125 No Hit GGAACGACTACACCCTACACCTACAACATGTCTTCGCACTCGCAATCCTA 5 0.125 No Hit CAGTAGCCGATCCAAGAACAGATCACCAACAAAAGCTTCTTGAGAGTCTT 5 0.125 No Hit CAACCACCACAACCGAACTTCTCTCGTCCTTATCTCCATTCTTCCATAGC 5 0.125 No Hit GTTACTGGAGAGGATGATGGAGGATAATGAGAGAATGATGGGATTGATGG 5 0.125 No Hit TGGAAGCAAGAAAATCAAAATGAACATTTCAGCAGGATCAAAGAAGTGGG 5 0.125 No Hit TTCTGATCCTCGTCATTCCCCTGAAAATATGTATGGATCTCCTTTGAGTG 5 0.125 No Hit GCTTGAGCCAGCTTACGCTGATTACTACAAGAAGGTCTTGGGCAGAAGGG 5 0.125 No Hit TGACAGCACAGTCTCCAAAGCCCTCATGCCACAGTGTCATCACTGGGAAT 5 0.125 No Hit GATATCTTATGATTTGTTTCTTTTCCTTTAACCTAGGATGGCTGGGGCTG 5 0.125 No Hit CCGCTCGCTCTTGTTGAACCAACTCAACTGCAAAGCTGTCGCTTCTTCTT 5 0.125 No Hit GTTTGCCAATGAAGGATTTCTGAGGGGTCAAAAGCAGCTTCTAAGGAATA 5 0.125 No Hit CGAGGATATTCGAAAACTAGAACATGGAGTTCATGTAGTTTCTGGGACTC 5 0.125 No Hit CTTGTATATCAGGACCTAAAAGGCCACATGATCGAGTTCCTCTGAGAGAA 5 0.125 No Hit CAGATATCATGTATTGGCATATAAAAAGGTGCCCAGTAATGATAGTACAG 5 0.125 No Hit CGATGTACAACGAGAAGGGGCTTGGTCTAGCGCGCACGCGCACGCGTTTG 5 0.125 No Hit CAAGGAGCTTATTAACCGTGGAGGGGAGAAGATATCACCAATTGAAGTGG 5 0.125 No Hit CAGGGCTTTGTGGAAAGCAACATGGCAAAGCATCATCCTGATTTGATTAT 5 0.125 No Hit CTTCAAGGACTTCCCTTTCCCTTCATCCTCACTTGTCTCTCATGACAATT 5 0.125 No Hit GTGGGTGTCACCGCCGGCAGGGAAGGGAAGAGGGGAGGAGGCTTTTACAG 5 0.125 No Hit GCTTCTCAATAACATCCGCTCCTAAGGCAACAGAAACTACTGGGGCGAGT 5 0.125 No Hit AGGCTTTGAAAGCTGTAGGCCAGATTTGGCTGCTATCAAACTTTGATTCG 5 0.125 No Hit TGGAAATGCTGGGTTGGGGTATGGCAGTGGCAATCAGTTTGGTGCCAATG 5 0.125 No Hit GTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTGTAAGTATGAACTA 5 0.125 No Hit ATTAGGGTTAGGTTTCTCCCAGTTTCGTTCCTCCTTCCAATCGTAGAAAA 5 0.125 No Hit AATCAACTCAGGCTTGATCTCATGTTTCAGATCGTAAAGGCAAGAGCAGA 5 0.125 No Hit CGAAAAATCTTGATTCTCTTTGTGCAACAAATGGCTAATGAAGTGGTTCT 5 0.125 No Hit TACAAAGAGAGGAGCTGTGGGCGAAACAAGCACCAGAAAGGCAAAGGATT 5 0.125 No Hit GCTGAAGGGGTTATGTCTCTTTATAAGGGCTTTATACCTACAATTTCAAG 5 0.125 No Hit GGATCATTCACATGGTTGCTGGGTTAGGCTTTGAGTGGGCCTTAAGCCCA 5 0.125 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0125 0.0 0.0 0.0 0.0 60-61 0.025 0.0 0.0 0.0 0.0 62-63 0.025 0.0 0.0 0.0 0.0 64-65 0.025 0.0 0.0 0.0 0.0 66-67 0.025 0.0 0.0 0.0 0.0 68-69 0.025 0.0 0.0 0.0 0.0 70-71 0.037500000000000006 0.0 0.0 0.0 0.0 72-73 0.05 0.0 0.0 0.0 0.0 74-75 0.05 0.0 0.0 0.0 0.0 76-77 0.07500000000000001 0.0 0.0 0.0 0.0 78-79 0.1 0.0 0.0 0.0 0.0 80-81 0.225 0.0 0.0 0.0 0.0 82-83 0.2875 0.0 0.0 0.0 0.0 84-85 0.4125 0.0 0.0 0.0 0.0 86-87 0.575 0.0 0.0 0.0 0.0 88-89 0.7625 0.0 0.0 0.0 0.0 90-91 1.175 0.0 0.0 0.0 0.0 92-93 1.425 0.0 0.0 0.0 0.0 94-95 1.6375000000000002 0.0 0.0 0.0 0.0 96-97 2.1500000000000004 0.0 0.0 0.0 0.0 98-99 2.5 0.0 0.0 0.0 0.0 100-101 2.8875 0.0 0.0 0.0 0.0 102-103 3.275 0.0 0.0 0.0 0.0 104-105 3.7125 0.0 0.0 0.0 0.0 106-107 4.3125 0.0 0.0 0.0 0.0 108-109 4.6 0.0 0.0 0.0 0.0 110-111 5.2 0.0 0.0 0.0 0.0 112-113 6.05 0.0 0.0 0.0 0.0 114-115 6.6125 0.0 0.0 0.0 0.0 116-117 8.1625 0.0 0.0 0.0 0.0 118-119 9.05 0.0 0.0 0.0 0.0 120-121 9.9 0.0 0.0 0.0 0.0 122-123 10.9875 0.0 0.0 0.0 0.0 124-125 12.05 0.0 0.0 0.0 0.0 126-127 13.0 0.0 0.0 0.0 0.0 128-129 13.9375 0.0 0.0 0.0 0.0 130-131 15.1375 0.0 0.0 0.0 0.0 132-133 16.175 0.0 0.0 0.0 0.0 134-135 17.25 0.0 0.0 0.0 0.0 136-137 18.325000000000003 0.0 0.0 0.0 0.0 138-139 19.487499999999997 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TTTTGGG 10 0.006830828 145.0 8 GTTTTGG 10 0.006830828 145.0 7 TTTGGGT 10 0.006830828 145.0 9 AGGAGGG 10 0.006830828 145.0 1 GATCGGA 70 7.343502E-4 14.5 135-139 GAAGAGC 75 0.0012377208 13.533334 140-144 >>END_MODULE Read 1854088 spots for SRR26075355.sra Written 1854088 spots for SRR26075355.sra Read 1854088 spots for SRR26075355.sra Written 1854088 spots for SRR26075355.sra Read 1854088 spots for SRR26075355.sra Written 1854088 spots for SRR26075355.sra Read 1854088 spots for SRR26075355.sra Written 1854088 spots for SRR26075355.sra Read 1854088 spots for SRR26075355.sra Written 1854088 spots for SRR26075355.sra Read 1854088 spots for SRR26075355.sra Written 1854088 spots for SRR26075355.sra Read 1854088 spots for SRR26075355.sra Written 1854088 spots for SRR26075355.sra Read 1854088 spots for SRR26075355.sra Written 1854088 spots for SRR26075355.sra Read 1854088 spots for SRR26075355.sra Written 1854088 spots for SRR26075355.sra Read 1854088 spots for SRR26075355.sra Written 1854088 spots for SRR26075355.sra Read 1854088 spots for SRR26075355.sra Written 1854088 spots for SRR26075355.sra Read 1854088 spots for SRR26075355.sra Written 1854088 spots for SRR26075355.sra Read 1854088 spots for SRR26075355.sra Written 1854088 spots for SRR26075355.sra Read 1854088 spots for SRR26075355.sra Written 1854088 spots for SRR26075355.sra Read 1854088 spots for SRR26075355.sra Written 1854088 spots for SRR26075355.sra Read 1854088 spots for SRR26075355.sra Written 1854088 spots for SRR26075355.sra Read 1854094 spots for SRR26075355.sra Written 1854094 spots for SRR26075355.sra Read 1854088 spots for SRR26075355.sra Written 1854088 spots for SRR26075355.sra Read 1854088 spots for SRR26075355.sra Written 1854088 spots for SRR26075355.sra Read 1854088 spots for SRR26075355.sra Written 1854088 spots for SRR26075355.sra SRR ids: ['SRR26075355.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_gafnq4li SRR26075355.sra spots: 37081766 blocks: [[1, 1854088], [1854089, 3708176], [3708177, 5562264], [5562265, 7416352], [7416353, 9270440], [9270441, 11124528], [11124529, 12978616], [12978617, 14832704], [14832705, 16686792], [16686793, 18540880], [18540881, 20394968], [20394969, 22249056], [22249057, 24103144], [24103145, 25957232], [25957233, 27811320], [27811321, 29665408], [29665409, 31519496], [31519497, 33373584], [33373585, 35227672], [35227673, 37081766]] SRR26075355 file size 13694394 SRR26075355 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o SRR26075355 SRR26075355_1.fastq SRR26075355_2.fastq Input file: SRR26075355_1.fastq Paired file: SRR26075355_2.fastq trimmed: SRR26075355-trimmed-pair1.fastq, SRR26075355-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Tue Feb 11 21:46:14 2025 >> started Tue Feb 11 21:46:57 2025 >> done (43.036s) 37081766 read pairs processed; of these: 112 ( 0.00%) short read pairs filtered out after trimming by size control 206198 ( 0.56%) empty read pairs filtered out after trimming by size control 36875456 (99.44%) read pairs available; of these: 9838084 (26.68%) trimmed read pairs available after processing 27037372 (73.32%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 18 6 0.00% 19 6 0.00% 20 17 0.00% 21 18 0.00% 22 30 0.00% 23 31 0.00% 24 23 0.00% 25 28 0.00% 26 40 0.00% 27 31 0.00% 28 61 0.00% 29 55 0.00% 30 72 0.00% 31 70 0.00% 32 74 0.00% 33 86 0.00% 34 87 0.00% 35 124 0.00% 36 107 0.00% 37 107 0.00% 38 135 0.00% 39 182 0.00% 40 183 0.00% 41 218 0.00% 42 198 0.00% 43 237 0.00% 44 211 0.00% 45 318 0.00% 46 223 0.00% 47 309 0.00% 48 331 0.00% 49 415 0.00% 50 438 0.00% 51 551 0.00% 52 561 0.00% 53 668 0.00% 54 654 0.00% 55 727 0.00% 56 778 0.00% 57 840 0.00% 58 1078 0.00% 59 1273 0.00% 60 1331 0.00% 61 1660 0.00% 62 1871 0.01% 63 2067 0.01% 64 2437 0.01% 65 2495 0.01% 66 2739 0.01% 67 3136 0.01% 68 3482 0.01% 69 4303 0.01% 70 4733 0.01% 71 5312 0.01% 72 5771 0.02% 73 7131 0.02% 74 8050 0.02% 75 9123 0.02% 76 10367 0.03% 77 10950 0.03% 78 12674 0.03% 79 13875 0.04% 80 15602 0.04% 81 17479 0.05% 82 19390 0.05% 83 21699 0.06% 84 24817 0.07% 85 27696 0.08% 86 30837 0.08% 87 32207 0.09% 88 34951 0.09% 89 37161 0.10% 90 39764 0.11% 91 44031 0.12% 92 46580 0.13% 93 50582 0.14% 94 56242 0.15% 95 60857 0.17% 96 64509 0.17% 97 68611 0.19% 98 71347 0.19% 99 74219 0.20% 100 78416 0.21% 101 81231 0.22% 102 85576 0.23% 103 89919 0.24% 104 96072 0.26% 105 102267 0.28% 106 107633 0.29% 107 110016 0.30% 108 116161 0.32% 109 118701 0.32% 110 119168 0.32% 111 123897 0.34% 112 128304 0.35% 113 131650 0.36% 114 134820 0.37% 115 143285 0.39% 116 146467 0.40% 117 152030 0.41% 118 158310 0.43% 119 159525 0.43% 120 162571 0.44% 121 164198 0.45% 122 167051 0.45% 123 169758 0.46% 124 176265 0.48% 125 179895 0.49% 126 183953 0.50% 127 191595 0.52% 128 192592 0.52% 129 196240 0.53% 130 199005 0.54% 131 200116 0.54% 132 201366 0.55% 133 207261 0.56% 134 207277 0.56% 135 213132 0.58% 136 217766 0.59% 137 217981 0.59% 138 221072 0.60% 139 227198 0.62% 140 226275 0.61% 141 228745 0.62% 142 231981 0.63% 143 231041 0.63% 144 232405 0.63% 145 237232 0.64% 146 235795 0.64% 147 240826 0.65% 148 241101 0.65% 149 242238 0.66% 150 247016 0.67% 151 27037372 73.32% 36875456 reads passed initial QC criterion=sequence-density sequence-density=0.70 sequence-density-rank=1 fanout-score=3.73 fanout-score-rank=25 prefix-density=0.76 prefix-fanout=3.5 sequence=GCATTCTCAGGCAGCCTAAACCTCCTC criterion=fanout-score sequence-density=0.19 sequence-density-rank=19 fanout-score=45.49 fanout-score-rank=1 prefix-density=1.12 prefix-fanout=7.8 sequence=TCATCTTCAATTTC criterion=sequence-density sequence-density=0.37 sequence-density-rank=1 fanout-score=13.04 fanout-score-rank=7 prefix-density=0.82 prefix-fanout=5.9 sequence=GTGGAGAAGGAAGACAAGAACGA criterion=fanout-score sequence-density=0.18 sequence-density-rank=23 fanout-score=57.24 fanout-score-rank=1 prefix-density=0.60 prefix-fanout=17.5 sequence=TGATGAGGATGAGG SRR26075355 testing PE reads STAR mapping to Ensembl genome Started job on | Feb 11 21:47:45 Started mapping on | Feb 11 21:47:45 Finished on | Feb 11 21:55:29 Mapping speed, Million of reads per hour | 286.10 Number of input reads | 36875456 Average input read length | 288 UNIQUE READS: Uniquely mapped reads number | 31609853 Uniquely mapped reads % | 85.72% Average mapped length | 286.92 Number of splices: Total | 25232474 Number of splices: Annotated (sjdb) | 24518156 Number of splices: GT/AG | 24777494 Number of splices: GC/AG | 333824 Number of splices: AT/AC | 28810 Number of splices: Non-canonical | 92346 Mismatch rate per base, % | 0.39% Deletion rate per base | 0.03% Deletion average length | 3.20 Insertion rate per base | 0.02% Insertion average length | 2.78 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 1109752 % of reads mapped to multiple loci | 3.01% Number of reads mapped to too many loci | 120879 % of reads mapped to too many loci | 0.33% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 10.54% % of reads unmapped: other | 0.40% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 4155851 4155851 4155851 N_multimapping 1109752 1109752 1109752 N_noFeature 737853 31266677 957720 N_ambiguous 310861 1789 186539 UnstrandedReadsAssigned:30561139 PositiveStrandReadsAssigned:341387 NegativeStrandReadsAssigned:30465594 Dataset is classified negative stranded MeadianReadLen=151 20thPercentileLength=139 echo kmer=135 SRR26075355 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,400 [index] number of k-mers: 62,057,036 [index] number of equivalence classes: 130,681 [quant] running in paired-end mode [quant] will process pair 1: SRR26075355-trimmed-pair1.fastq SRR26075355-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 36,875,456 reads, 31,218,056 reads pseudoaligned [quant] estimated average fragment length: 182.124 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,219 rounds 52401 SRR26075355.ke.tsv 34699 SRR26075355.se.tsv 87100 total ==> SRR26075355.ke.tsv <== target_id length eff_length est_counts tpm Potri.005G200100.1.v4.1 2018 1836.88 5759 93.0726 Potri.005G024800.1.v4.1 1035 853.876 8776 305.11 Potri.004G059700.1.v4.1 961 779.876 18 0.685175 Potri.007G009000.2.v4.1 1416 1234.88 0 0 Potri.003G141000.2.v4.1 2943 2761.88 1332 14.3171 Potri.016G087400.1.v4.1 270 101.498 3178 929.505 Potri.015G069301.1.v4.1 564 383.051 0 0 Potri.010G195200.1.v4.1 1773 1591.88 174 3.24485 Potri.012G127500.1.v4.1 977 795.876 6679 249.127 ==> SRR26075355.se.tsv <== Potri.001G166300.v4.1 0 Potri.001G448400.v4.1 50 Potri.001G233950.v4.1 0 Potri.001G122700.v4.1 217 Potri.001G212900.v4.1 0 Potri.001G182400.v4.1 0 Potri.001G256600.v4.1 0 Potri.001G040500.v4.1 19 Potri.001G416900.v4.1 0 Potri.001G452600.v4.1 704 SRR26075355 completed mapping pipeline successfully