Starting /dee2/code/volunteer_pipeline.sh SRR26075356 current disk space = 3052542758912 free memory = 1578643560 SRR26075356 SRAfilesize 624c3a435969b6945eefbf11f5fe7513 SRR26075356.sra SRR26075356.sra file validated SRR26075356 is paired end SRR26075356 is conventional basespace SRR26075356 read1 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR26075356_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.563 37.0 37.0 37.0 37.0 37.0 2 36.5765 37.0 37.0 37.0 37.0 37.0 3 36.67 37.0 37.0 37.0 37.0 37.0 4 36.597 37.0 37.0 37.0 37.0 37.0 5 36.6555 37.0 37.0 37.0 37.0 37.0 6 36.6955 37.0 37.0 37.0 37.0 37.0 7 36.6485 37.0 37.0 37.0 37.0 37.0 8 36.601 37.0 37.0 37.0 37.0 37.0 9 36.6755 37.0 37.0 37.0 37.0 37.0 10-14 36.6725 37.0 37.0 37.0 37.0 37.0 15-19 36.5997 37.0 37.0 37.0 37.0 37.0 20-24 36.5718 37.0 37.0 37.0 37.0 37.0 25-29 36.5286 37.0 37.0 37.0 37.0 37.0 30-34 36.4834 37.0 37.0 37.0 37.0 37.0 35-39 36.46320000000001 37.0 37.0 37.0 37.0 37.0 40-44 36.393899999999995 37.0 37.0 37.0 37.0 37.0 45-49 36.3414 37.0 37.0 37.0 37.0 37.0 50-54 36.302499999999995 37.0 37.0 37.0 37.0 37.0 55-59 36.2919 37.0 37.0 37.0 37.0 37.0 60-64 36.2315 37.0 37.0 37.0 37.0 37.0 65-69 36.1903 37.0 37.0 37.0 37.0 37.0 70-74 36.1919 37.0 37.0 37.0 37.0 37.0 75-79 36.1674 37.0 37.0 37.0 37.0 37.0 80-84 36.085800000000006 37.0 37.0 37.0 37.0 37.0 85-89 35.9219 37.0 37.0 37.0 37.0 37.0 90-94 35.986399999999996 37.0 37.0 37.0 37.0 37.0 95-99 35.86409999999999 37.0 37.0 37.0 37.0 37.0 100-104 35.8964 37.0 37.0 37.0 37.0 37.0 105-109 35.799800000000005 37.0 37.0 37.0 37.0 37.0 110-114 35.6786 37.0 37.0 37.0 37.0 37.0 115-119 35.5631 37.0 37.0 37.0 37.0 37.0 120-124 35.6483 37.0 37.0 37.0 37.0 37.0 125-129 35.5245 37.0 37.0 37.0 37.0 37.0 130-134 35.3875 37.0 37.0 37.0 34.6 37.0 135-139 35.3229 37.0 37.0 37.0 34.6 37.0 140-144 35.1522 37.0 37.0 37.0 29.8 37.0 145-149 35.202600000000004 37.0 37.0 37.0 27.4 37.0 150-151 35.052 37.0 37.0 37.0 25.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.0 1101 2 0.0 1101 3 0.0 1101 4 0.0 1101 5 0.0 1101 6 0.0 1101 7 0.0 1101 8 0.0 1101 9 0.0 1101 10-14 0.0 1101 15-19 0.0 1101 20-24 0.0 1101 25-29 0.0 1101 30-34 0.0 1101 35-39 0.0 1101 40-44 0.0 1101 45-49 0.0 1101 50-54 0.0 1101 55-59 0.0 1101 60-64 0.0 1101 65-69 0.0 1101 70-74 0.0 1101 75-79 0.0 1101 80-84 0.0 1101 85-89 0.0 1101 90-94 0.0 1101 95-99 0.0 1101 100-104 0.0 1101 105-109 0.0 1101 110-114 0.0 1101 115-119 0.0 1101 120-124 0.0 1101 125-129 0.0 1101 130-134 0.0 1101 135-139 0.0 1101 140-144 0.0 1101 145-149 0.0 1101 150-151 0.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 21 2.0 22 4.0 23 2.0 24 2.0 25 8.0 26 4.0 27 13.0 28 18.0 29 26.0 30 25.0 31 25.0 32 60.0 33 95.0 34 170.0 35 458.0 36 2917.0 37 171.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 37.86967418546366 16.06516290726817 8.170426065162907 37.89473684210527 2 18.675 14.025000000000002 35.9 31.4 3 16.7 17.2 30.325000000000003 35.775 4 22.075 22.075 25.525 30.325000000000003 5 23.575 26.3 26.0 24.125 6 23.05 31.25 23.9 21.8 7 15.9 30.175 37.925 16.0 8 17.8 26.25 32.4 23.549999999999997 9 17.424999999999997 23.974999999999998 33.425 25.174999999999997 10-14 19.580000000000002 30.37 27.529999999999998 22.52 15-19 19.145 28.835 27.815 24.205 20-24 19.295 28.28 28.01 24.415 25-29 20.05 28.904999999999998 27.034999999999997 24.01 30-34 19.3 28.744999999999997 28.015 23.94 35-39 19.939999999999998 28.285 27.810000000000002 23.965 40-44 20.39 29.4 26.75 23.46 45-49 19.61 28.835 27.82 23.735 50-54 19.705000000000002 26.93 29.035 24.33 55-59 19.994999999999997 28.625 27.005000000000003 24.375 60-64 19.915 28.29 27.875 23.919999999999998 65-69 20.59 29.104999999999997 27.089999999999996 23.215 70-74 19.935 28.645 26.840000000000003 24.58 75-79 19.665 28.215 27.425 24.695 80-84 20.455000000000002 28.585 26.845000000000002 24.115000000000002 85-89 19.715 27.689999999999998 27.505000000000003 25.09 90-94 20.635 28.835 26.540000000000003 23.990000000000002 95-99 19.765 28.105000000000004 27.694999999999997 24.435000000000002 100-104 20.505000000000003 27.815 28.26 23.419999999999998 105-109 19.794999999999998 28.48 27.425 24.3 110-114 20.855 27.12 27.779999999999998 24.245 115-119 21.224999999999998 27.57 26.815 24.39 120-124 20.925 28.64 26.745 23.69 125-129 21.19 27.500000000000004 27.365000000000002 23.945 130-134 21.18 27.389999999999997 27.025 24.404999999999998 135-139 20.78 27.644999999999996 27.245 24.33 140-144 20.275000000000002 27.22 27.105 25.4 145-149 21.085 27.905 27.025 23.985 150-151 21.6125 27.9375 25.412499999999998 25.0375 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.5 19 0.5 20 0.5 21 1.0 22 2.5 23 3.0 24 5.0 25 5.5 26 7.0 27 10.0 28 14.0 29 17.0 30 14.5 31 20.0 32 24.5 33 29.5 34 42.5 35 63.0 36 88.0 37 103.0 38 127.0 39 166.5 40 193.0 41 214.5 42 245.5 43 258.5 44 249.0 45 260.0 46 270.0 47 263.0 48 247.0 49 199.0 50 173.5 51 156.0 52 117.5 53 97.5 54 69.0 55 51.5 56 43.0 57 24.5 58 22.5 59 17.5 60 14.0 61 12.0 62 8.0 63 9.5 64 7.5 65 6.0 66 5.5 67 5.5 68 5.5 69 4.5 70 3.5 71 1.5 72 0.0 73 0.0 74 0.0 75 0.0 76 0.0 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.25 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150-151 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 63.65 #Duplication Level Percentage of deduplicated Percentage of total 1 65.78947368421053 41.875 2 20.620581304006286 26.25 3 8.366064414768264 15.975 4 3.102906520031422 7.9 5 1.178318931657502 3.75 6 0.432050274941084 1.6500000000000001 7 0.2749410840534171 1.225 8 0.15710919088766695 0.8 9 0.0 0.0 >10 0.07855459544383347 0.575 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTGGCTTTATTGGAGGAGTCACAGGAGGCTTTGGTGGCTTTATTGGAGGA 13 0.325 No Hit CCGTAAAACTCAATTGTCCTTGCAGTTCCTTGTGTAGTTTGAAGATAATC 10 0.25 No Hit CTTTCTTCAAATTCTGAATTTTATCACTTAAATTGATAATTTTCTGCAAA 8 0.2 No Hit GTTGCAAGCAATACGTGCCGCTTCAAGAGCTTCACTGGAGACGTTCTCCT 8 0.2 No Hit CAGGTCCAGCACCTTGATACATCTTGGCAATAATAGGATTGCAGATGCTC 8 0.2 No Hit GCTCTTGTCAAATTTGCAAGTGCCACCGTCAGTTCCAGTGTAAGGATAGT 8 0.2 No Hit GGCTTCACCCACACCTTGGCTTCATACACTTTCTTGTGACCCCCTTCAGT 7 0.17500000000000002 No Hit GCTACTGCACAAGGAACAGAGAATAGACCCACCTATACATACCCGGATCT 7 0.17500000000000002 No Hit CTCTGCAATTACCACGAATACATCTTTCAGTGACCACCTACGTCTCTCAC 7 0.17500000000000002 No Hit TGCCACATTTCTCAACTCCATATCTCTAAACTTCTCAAATGCTTTTTGGG 7 0.17500000000000002 No Hit TAGGTGAGCAACTTGGATAATTTGTAGGCACAAGCAATCTGAGGGGCTGA 7 0.17500000000000002 No Hit GTTTCAAGTTTTTGGTCATCTACTTGGCTATTGACCTGAGTAGGCGGTAA 7 0.17500000000000002 No Hit GTGACATTGAGGAAGGGATTGCCCGTGTCGATTGCCACATTAAGTTTTGG 7 0.17500000000000002 No Hit TGCACAAGCTACAGGGTGACCAGAGTAAGTGAATCCATGAGAGAAAGAAC 6 0.15 No Hit ATTCTATCTTCCTAAATCAATCTCCGCCTTCTAGGTGCACGAGAGTCCCC 6 0.15 No Hit GCTGTTGTTGCTGCCTAAGCCTTACAATCTCTGCCATTAACACGTTTCGG 6 0.15 No Hit TCCGCTGCTAAAATTAGTATGGGTTATATCGGCTGCGTCCACGGCCGCCA 6 0.15 No Hit ACTCGATAGAAGACAGGCTCCCATACACATGAAATTATCACAACACCTGC 6 0.15 No Hit GCTTGCCAGTGTCCAAATTCACTCTTGACTCTGTCTTCTTTAGGAGTTCA 6 0.15 No Hit CCATGCTATTGTTCACATTGAATTATACATTCTAGCCTTCTGTACATTCC 6 0.15 No Hit AATCGATCTCTGGTCCCATGCTCTCGGATAATATCAAGATTGAACAACCG 6 0.15 No Hit CTCTTGTGACTTTTTGGCCATATTTCCTCCATTGATATCCATCTTTCACA 6 0.15 No Hit ATTCGATATCATTCTCTTCCTCTTCCTCTGATCCATCAATGCCTGCAAAT 6 0.15 No Hit CACCCGGAAGCGGCCAGTCCTTTCCTTTCAAAACTCAAACTCAAGGCATG 6 0.15 No Hit CTCTGCAGATCCCTTTTTTTTTCTTCTGTTTCAGGTGGTCGGCGGTAGGA 5 0.125 No Hit GCTCTGGACCCATCTCCTCGTTTCTCATCTCACAAATGTTATGCAGCACA 5 0.125 No Hit GTTGGCGACTTATAACATATGACAGCTACCACGCCACCTAAAACTATCCC 5 0.125 No Hit AGCAGGTCACCAACTTAAGCAACTGGTGGGCGGAGCACATGATCTTGCGA 5 0.125 No Hit TTTTTTTGCAGACATGAATCATCAATAACTTATTATCATTTCAAAACACA 5 0.125 No Hit ACATGACGAAGCTCAGCTGCGGAGATGAAACCATTCTGATCCTTGTCAAA 5 0.125 No Hit CACCTCTTCATTTATATCACCTGACAGTCTCTTCAGCAGAATGACTCTAT 5 0.125 No Hit GTTGCAAAGCTTCTTTTCCACCAAGTGGTTGCCCTCCTGCCCAGTATATT 5 0.125 No Hit ACCAAATTCTGCGTCTAACAGTATGTTGTTTGATTTTACATCCCGGTGAA 5 0.125 No Hit GTTTTTTCTGAAATATCTGGTTTTATCCACCACTGAGCCATTTGTTTCCA 5 0.125 No Hit GATAAAACGTGATTGCCTCAATCTCCTTTAGTTGGAGGTTGCACGCATGA 5 0.125 No Hit GTGGAACATGAGGTTGCATATGGCTTTTTCTGCTAGTTTATTACCTCGTT 5 0.125 No Hit ATCGGTATCAAATACATTGAAGAGCTCATGGATCTCATCCTCATCCAAGT 5 0.125 No Hit GCCATGAATTCATGTTCGGTCCGTTCTTTCCCTCCTGGGTTTTGAGTCAT 5 0.125 No Hit CCCAACGCCATTAGTTTCTCATCTCTATCCAGACCTGCCAAGAAAAAACA 5 0.125 No Hit AGCCTGGCAACCACTCAAAGCTTCCTAATAGCGACCAACTCCCCAGACTC 5 0.125 No Hit CCTGACCCAGGCGACGAACCCTCGAAAATAAATGGCAAATAGCCAGTTCC 5 0.125 No Hit CCTAGAAATTTTGGGGGGGCGTACATTTTTCTGCCCCAAAAAGAGTGTTC 5 0.125 No Hit CACAATGAGTCACAAACCCTCCAACCGAGCCGTGGTTGAGTATTTCAACT 5 0.125 No Hit CCAGTCCTTGAAGGAGCCCCGGTTATCGGTCCAGATTTCCTAGACTTTGA 5 0.125 No Hit GCATACACATCATTTCAGATGTAGCCTTCCTATCCATCGAATGGTCACTG 5 0.125 No Hit CTCTACATATATTACATCTTAATTACGACACATCTCACAGACCATCAACA 5 0.125 No Hit ACCTTGTGCAACCCCAATTTCATCCCGTCGACAGCACATGAAGTGTCCAC 5 0.125 No Hit GCATCGACCTCTTTCGTGCTCATCTTACCACGGAACATGGCTGAAGCAGT 5 0.125 No Hit GTCGCTGAATCCTGTGAGCTGAAGAATGCGGTTAAGCTTATTTGCATCAT 5 0.125 No Hit ATCCCCAGCAAGTTTTCTGGCAAGGTACAAGAATGGCTTCTCAAAATTAT 5 0.125 No Hit TGAGTACATTTTAGTCCCCACACATCAAACAATACTACATCAGCACGCCA 5 0.125 No Hit GGATCTCTTGCATTTTGTTCCAAAGTCATTCAACTTCTTTTGGCTAAAGA 5 0.125 No Hit AGCAAGTAAGTTTGACCACAGAACAATACAAAAAAGACCCTTGAACTGAA 5 0.125 No Hit AGCCTCCATTCGAAAAGATAATACATCGCCCTGGATTCATTTAATTTTCA 5 0.125 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.025 0.0 0.0 0.0 0.0 42-43 0.025 0.0 0.0 0.0 0.0 44-45 0.025 0.0 0.0 0.0 0.0 46-47 0.025 0.0 0.0 0.0 0.0 48-49 0.025 0.0 0.0 0.0 0.0 50-51 0.025 0.0 0.0 0.0 0.0 52-53 0.025 0.0 0.0 0.0 0.0 54-55 0.025 0.0 0.0 0.0 0.0 56-57 0.025 0.0 0.0 0.0 0.0 58-59 0.025 0.0 0.0 0.0 0.0 60-61 0.025 0.0 0.0 0.0 0.0 62-63 0.025 0.0 0.0 0.0 0.0 64-65 0.025 0.0 0.0 0.0 0.0 66-67 0.025 0.0 0.0 0.0 0.0 68-69 0.025 0.0 0.0 0.0 0.0 70-71 0.05 0.0 0.0 0.0 0.0 72-73 0.05 0.0 0.0 0.0 0.0 74-75 0.05 0.0 0.0 0.0 0.0 76-77 0.05 0.0 0.0 0.0 0.0 78-79 0.075 0.0 0.0 0.0 0.0 80-81 0.075 0.0 0.0 0.0 0.0 82-83 0.075 0.0 0.0 0.0 0.0 84-85 0.15000000000000002 0.0 0.0 0.0 0.0 86-87 0.225 0.0 0.0 0.0 0.0 88-89 0.3125 0.0 0.0 0.0 0.0 90-91 0.4 0.0 0.0 0.0 0.0 92-93 0.5249999999999999 0.0 0.0 0.0 0.0 94-95 0.7250000000000001 0.0 0.0 0.0 0.0 96-97 0.8875 0.0 0.0 0.0 0.0 98-99 1.0125 0.0 0.0 0.0 0.0 100-101 1.1 0.0 0.0 0.0 0.0 102-103 1.225 0.0 0.0 0.0 0.0 104-105 1.3125 0.0 0.0 0.0 0.0 106-107 1.6375000000000002 0.0 0.0 0.0 0.0 108-109 1.875 0.0 0.0 0.0 0.0 110-111 2.025 0.0 0.0 0.0 0.0 112-113 2.3 0.0 0.0 0.0 0.0 114-115 2.4749999999999996 0.0 0.0 0.0 0.0 116-117 2.825 0.0 0.0 0.0 0.0 118-119 3.1875 0.0 0.0 0.0 0.0 120-121 3.475 0.0 0.0 0.0 0.0 122-123 4.0375 0.0 0.0 0.0 0.0 124-125 4.4625 0.0 0.0 0.0 0.0 126-127 4.8125 0.0 0.0 0.0 0.0 128-129 5.325 0.0 0.0 0.0 0.0 130-131 6.0375 0.0 0.0 0.0 0.0 132-133 6.6625 0.0 0.0 0.0 0.0 134-135 7.15 0.0 0.0 0.0 0.0 136-137 7.6625 0.0 0.0 0.0 0.0 138-139 8.3125 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CATGAAA 10 0.006830828 145.0 9 CCAGTGC 10 0.006830828 145.0 1 TAGCCAC 10 0.006830828 145.0 145 CAGACCC 10 0.006830828 145.0 2 TTGAAGG 10 0.006830828 145.0 8 AGACCCC 10 0.006830828 145.0 3 AAGAATT 20 0.00593511 29.0 30-34 >>END_MODULE SRR26075356 read2 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR26075356_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.4225 37.0 37.0 37.0 37.0 37.0 2 36.387 37.0 37.0 37.0 37.0 37.0 3 36.371 37.0 37.0 37.0 37.0 37.0 4 36.3935 37.0 37.0 37.0 37.0 37.0 5 36.402 37.0 37.0 37.0 37.0 37.0 6 36.327 37.0 37.0 37.0 37.0 37.0 7 36.3125 37.0 37.0 37.0 37.0 37.0 8 36.502 37.0 37.0 37.0 37.0 37.0 9 36.3495 37.0 37.0 37.0 37.0 37.0 10-14 36.36129999999999 37.0 37.0 37.0 37.0 37.0 15-19 36.3546 37.0 37.0 37.0 37.0 37.0 20-24 36.253600000000006 37.0 37.0 37.0 37.0 37.0 25-29 36.1896 37.0 37.0 37.0 37.0 37.0 30-34 36.0383 37.0 37.0 37.0 37.0 37.0 35-39 36.057900000000004 37.0 37.0 37.0 37.0 37.0 40-44 35.98819999999999 37.0 37.0 37.0 37.0 37.0 45-49 35.936099999999996 37.0 37.0 37.0 37.0 37.0 50-54 35.868100000000005 37.0 37.0 37.0 37.0 37.0 55-59 35.8834 37.0 37.0 37.0 37.0 37.0 60-64 35.8778 37.0 37.0 37.0 37.0 37.0 65-69 35.82430000000001 37.0 37.0 37.0 37.0 37.0 70-74 35.7347 37.0 37.0 37.0 37.0 37.0 75-79 35.743700000000004 37.0 37.0 37.0 37.0 37.0 80-84 35.73909999999999 37.0 37.0 37.0 37.0 37.0 85-89 35.7325 37.0 37.0 37.0 37.0 37.0 90-94 35.6331 37.0 37.0 37.0 37.0 37.0 95-99 35.7146 37.0 37.0 37.0 37.0 37.0 100-104 35.4798 37.0 37.0 37.0 37.0 37.0 105-109 35.6164 37.0 37.0 37.0 37.0 37.0 110-114 35.451699999999995 37.0 37.0 37.0 37.0 37.0 115-119 35.397099999999995 37.0 37.0 37.0 37.0 37.0 120-124 35.38 37.0 37.0 37.0 34.6 37.0 125-129 35.4018 37.0 37.0 37.0 37.0 37.0 130-134 35.2641 37.0 37.0 37.0 34.6 37.0 135-139 35.2231 37.0 37.0 37.0 32.2 37.0 140-144 35.142500000000005 37.0 37.0 37.0 29.8 37.0 145-149 35.1368 37.0 37.0 37.0 29.8 37.0 150-151 34.86425 37.0 37.0 37.0 25.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.0 1101 2 0.0 1101 3 0.0 1101 4 0.0 1101 5 0.0 1101 6 0.0 1101 7 0.0 1101 8 0.0 1101 9 0.0 1101 10-14 0.0 1101 15-19 0.0 1101 20-24 0.0 1101 25-29 0.0 1101 30-34 0.0 1101 35-39 0.0 1101 40-44 0.0 1101 45-49 0.0 1101 50-54 0.0 1101 55-59 0.0 1101 60-64 0.0 1101 65-69 0.0 1101 70-74 0.0 1101 75-79 0.0 1101 80-84 0.0 1101 85-89 0.0 1101 90-94 0.0 1101 95-99 0.0 1101 100-104 0.0 1101 105-109 0.0 1101 110-114 0.0 1101 115-119 0.0 1101 120-124 0.0 1101 125-129 0.0 1101 130-134 0.0 1101 135-139 0.0 1101 140-144 0.0 1101 145-149 0.0 1101 150-151 0.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 13 1.0 14 13.0 15 7.0 16 5.0 17 2.0 18 2.0 19 11.0 20 8.0 21 6.0 22 7.0 23 11.0 24 10.0 25 8.0 26 7.0 27 14.0 28 17.0 29 10.0 30 23.0 31 24.0 32 39.0 33 81.0 34 132.0 35 561.0 36 2752.0 37 249.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 41.699999999999996 21.099999999999998 12.1 25.1 2 27.975 27.075 25.8 19.15 3 22.925 29.975 29.5 17.599999999999998 4 24.125 34.65 22.0 19.225 5 26.375 35.699999999999996 21.025 16.900000000000002 6 22.0 36.6 22.400000000000002 19.0 7 21.975 22.6 37.3 18.125 8 23.724999999999998 25.7 26.525 24.05 9 23.05 26.625 27.900000000000002 22.425 10-14 24.08 29.494999999999997 24.965 21.46 15-19 24.785 28.225 26.05 20.94 20-24 24.675 28.515 26.13 20.68 25-29 25.16 27.52 26.88 20.44 30-34 24.305 28.599999999999998 26.655 20.44 35-39 24.834999999999997 26.955000000000002 27.33 20.880000000000003 40-44 24.775 27.700000000000003 27.055 20.47 45-49 24.610000000000003 27.634999999999998 27.235 20.52 50-54 24.345 28.025 27.189999999999998 20.44 55-59 23.43 27.474999999999998 28.265 20.830000000000002 60-64 24.55 27.79 27.384999999999998 20.275000000000002 65-69 25.230000000000004 27.49 27.575 19.705000000000002 70-74 25.465 28.42 26.02 20.095 75-79 24.515 28.78 26.61 20.095 80-84 24.37 29.32 26.96 19.35 85-89 24.54 27.650000000000002 26.900000000000002 20.91 90-94 24.89 27.779999999999998 27.355 19.975 95-99 24.425 28.825 26.63 20.119999999999997 100-104 24.565 27.47 27.500000000000004 20.465 105-109 25.94 28.744999999999997 25.7 19.615 110-114 24.75 28.115000000000002 26.584999999999997 20.549999999999997 115-119 24.215 28.34 27.445000000000004 20.0 120-124 24.275 28.48 27.060000000000002 20.185 125-129 25.040000000000003 28.115000000000002 27.24 19.605 130-134 25.095 28.515 26.61 19.78 135-139 25.585 28.465 26.619999999999997 19.33 140-144 25.61 27.765 26.66 19.965 145-149 26.224999999999998 28.46 26.119999999999997 19.195 150-151 26.0375 28.3625 26.474999999999998 19.125 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.5 8 0.5 9 0.5 10 1.0 11 0.5 12 0.5 13 1.0 14 1.0 15 2.5 16 3.0 17 1.0 18 1.0 19 2.0 20 2.0 21 4.0 22 3.5 23 1.0 24 1.5 25 4.0 26 6.0 27 4.0 28 2.5 29 4.0 30 8.5 31 11.0 32 18.5 33 24.5 34 33.0 35 53.0 36 66.5 37 85.0 38 97.0 39 132.0 40 181.0 41 230.5 42 248.0 43 249.5 44 296.0 45 288.0 46 276.5 47 285.5 48 248.0 49 199.5 50 172.5 51 145.0 52 118.0 53 94.5 54 69.5 55 66.5 56 51.5 57 29.5 58 28.0 59 29.0 60 21.5 61 9.5 62 12.0 63 17.0 64 8.0 65 4.5 66 3.5 67 1.5 68 2.0 69 2.0 70 2.5 71 3.0 72 1.5 73 2.0 74 1.5 75 0.0 76 0.0 77 0.5 78 0.5 79 0.0 80 1.5 81 1.5 82 0.0 83 1.0 84 1.0 85 0.0 86 0.5 87 1.0 88 1.0 89 1.0 90 0.5 91 0.5 92 1.5 93 1.5 94 1.0 95 0.5 96 0.5 97 0.5 98 0.0 99 1.0 100 4.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150-151 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 64.3 #Duplication Level Percentage of deduplicated Percentage of total 1 67.10730948678072 43.15 2 19.712286158631414 25.35 3 8.009331259720062 15.45 4 3.1104199066874028 8.0 5 1.0497667185069983 3.375 6 0.5443234836702955 2.1 7 0.19440124416796267 0.8750000000000001 8 0.19440124416796267 1.0 9 0.0 0.0 >10 0.07776049766718507 0.7000000000000001 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG 16 0.4 No Hit CAGTAAAGTAAGGAGACACGATGGCTAAGTTTGCTGTGGCTAATCTCTTG 12 0.3 No Hit ACTACGCCTACAACATGAGGAACACTGTGAAGGATGAGAAGATCAGCTCC 8 0.2 No Hit CTGCCGCCGATCCACCGCACAGAAGTCGCCATGGGTAGAAGACCTGCAAG 8 0.2 No Hit GATCCTTCCTACTACTGATCTACCTACTGATTATGACTGGCGCGATCACG 8 0.2 No Hit CCACTCTAAGTGCTTTTTCAAGGAGGCCTCGTTTCAATTCAAATCACCAT 8 0.2 No Hit TGGGGTTGAACCAAGTTTATTTACCTATAATTTTTTGCTTAATGGTTTGG 8 0.2 No Hit TACATTTAAATTCTACCACCTTGAAGTGGACGCCTGGGGGAGGGAGGTAG 7 0.17500000000000002 No Hit CTTTATCTCAACATACTCTTCTGAAACTGCTGATCAAGCCATTGCTGACA 7 0.17500000000000002 No Hit TCTAGAGCTAGAAAACTCTGGTCGAAGAATTGAAGCGAAGAAGAAAGAGA 7 0.17500000000000002 No Hit GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGT 7 0.17500000000000002 No Hit TGAAGATGTTGATTCAACTGCAGTAGCTGCTACTGCTGAAGGGGGTGAAG 7 0.17500000000000002 No Hit CTCTCACAGACCACACAACAACAAGGTGGAGGAGCTTGCATTGAGTTAGG 6 0.15 No Hit CTAATAAAACCATATAATGTAGAAGGAGGTTTAGGTGTATATTGTATTAC 6 0.15 No Hit CGTGTTTCTTGTGCTGCCGCTTCTGCAGACAATGCTGATTTGGACCCTGG 6 0.15 No Hit CAGTAGCAAAGGCCCTTTCCTCTGGTTACTTGCCAATAGGAGCTGTTATG 6 0.15 No Hit GTTGGTTACAGTTTAGTTGGGAGTTCATATGGTGCTCTTGGTGCAGGATT 6 0.15 No Hit CTACAACAAGAAACAGAATTCAGTGCTGGAGTTCAAGAGGGTGTTGAATG 6 0.15 No Hit TGTTGCCATGACTTGGAGGCGTACCTCCACCTGGTTGATGGGTTCATCGC 6 0.15 No Hit GAAACGATGAGAGTGAAAGTGAGGATGAGGCACCTAGACGGTCAAGGCAC 6 0.15 No Hit ATCCATTGATACGGCCGCGACACCTTGTTCAAGGGTTGGGTTGCCTGGTT 6 0.15 No Hit GTGCGGTTGCGGCAAATAACCCTGCATTATTGGCCATATCTGAGATCCGC 6 0.15 No Hit GAACCCATACCATAGGGAGGAATCACGAGCAGCCCCAGCCTGTTGATGAT 6 0.15 No Hit ACTCAATCGAGGAACAGAAACCCGGTTTACGAACCTCTTCTCTCCGATTC 6 0.15 No Hit GGAGAAAAGGAGCTTCTTGCCCCATCAAAGAAAAGAAAGTCTGAAAGCAG 6 0.15 No Hit TATATATTACCATTACTACTACTGTTGTCTGCATTAGTTTTGAGTCTGTT 6 0.15 No Hit ATCTGAGTTCCTAACTATGTTTTTTGTTGGATTTTCAGGCATTGTCTCTC 5 0.125 No Hit CATCTTCTCCGGCATCTGTAGTAGTTCTCACCTCCTCGGGTGATCGTGTC 5 0.125 No Hit GGAAAACAAAAATCACAGAGCAAAAAACAAAAGTTCTACTGCAGGACTAT 5 0.125 No Hit TGTCAGCTTATTACAACAGGAGGCATACTGAGAGGCATCAGAAGACATCG 5 0.125 No Hit GAATGAGCTTCGCGATGAGAAGCAGAGGCTGAAGGCAGAGAAGGAGAAGT 5 0.125 No Hit TACTTCTCTTCCTTGTTTACAACGAGGGAGAAGAGTCTGAGATTTGCAGA 5 0.125 No Hit TGGTTATCTTCGCCGAGCGATGACACACTCATCATTCTCCGAGGAGAACA 5 0.125 No Hit CCATTCAAATGGTGGAGACTTACTTGGATGTTCTCCTTCATTTCGAGATC 5 0.125 No Hit CTTCAATTTGCAATTATTTTGTGTGTGTACCGTGTTTTGATCTGTTGTTG 5 0.125 No Hit CCACATTCGTTAAGAGGCATCTTACCGGAGAGTTCGAGAAGAAATACGAG 5 0.125 No Hit CTAATCATCTCTCCCTCTCTCTCTCTTAAGAAAAAACCATTCCGCCTAAA 5 0.125 No Hit AGGGTATGAGTCAGCTGGAGTTGGAGGATGAAGTTCAAGATGATTTGAAA 5 0.125 No Hit GAGGCTTGTTGGATTGGCCTACAAGGTATAAGATAGCTTTGGATGCAGCT 5 0.125 No Hit GAAAGATGGGGTGATTTTGTTGTGGGATTTGGCTGAAGGAAAGAAGCTTT 5 0.125 No Hit GTGAGATTGTTGCTGTAAGAGAAGCAGAGAAGGTACTTTGATCTTGAAGT 5 0.125 No Hit GGAAACGGCTATTGGGTTGGAGAAAAGTGGTGTTAGGTTCTTGTGGGTGG 5 0.125 No Hit ATACGATGAGATTATCAATAATGAGAGGAAACAACGACCTGAACTCCTTA 5 0.125 No Hit GAAGGAAGGCCGCCGTTCGATCATTGCCAACGACTCCTCCAGATTCGACC 5 0.125 No Hit AAGGGCTCCAACAATGAGCTCAGTGGCCATGCCATATACTGGTGGTGATA 5 0.125 No Hit GGAAATGGAAAGGCCAAACTTTGTTGAGAATGGAGGGCTCAGATTGCCTA 5 0.125 No Hit GTTAAGACGTCAGAACAGTTTGATATAAGGAGGAGTCCTATATCAATCGC 5 0.125 No Hit GCAAAAATAACTGGTGGGGGTTCTGGTGGAACAGTTTGTGTAATCGGTAG 5 0.125 No Hit GATCTGTCACCCCAAGCTCGGGTTCTCCATCTCATTGAAAGCAAATTGAG 5 0.125 No Hit TGGAAGAAGAAAATACTGATCTTCCTCTGCAGAAGATATGTTTTAAGTAG 5 0.125 No Hit GGATGCACGAAACAAAGTACAGGAGGAGATCGGCCGCGAATTCGCTGCGA 5 0.125 No Hit GGAAATGGTACAATTGATTTTCCCGAGTTCCTGAACCTCATGGCAAGGAA 5 0.125 No Hit AACACGTGGGAGGAGATATGTTTGAAAGTGTTCCAAAAGGAGATGCTATT 5 0.125 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.025 0.0 0.0 0.0 0.0 42-43 0.025 0.0 0.0 0.0 0.0 44-45 0.025 0.0 0.0 0.0 0.0 46-47 0.025 0.0 0.0 0.0 0.0 48-49 0.025 0.0 0.0 0.0 0.0 50-51 0.025 0.0 0.0 0.0 0.0 52-53 0.025 0.0 0.0 0.0 0.0 54-55 0.025 0.0 0.0 0.0 0.0 56-57 0.025 0.0 0.0 0.0 0.0 58-59 0.025 0.0 0.0 0.0 0.0 60-61 0.025 0.0 0.0 0.0 0.0 62-63 0.025 0.0 0.0 0.0 0.0 64-65 0.025 0.0 0.0 0.0 0.0 66-67 0.025 0.0 0.0 0.0 0.0 68-69 0.025 0.0 0.0 0.0 0.0 70-71 0.05 0.0 0.0 0.0 0.0 72-73 0.05 0.0 0.0 0.0 0.0 74-75 0.05 0.0 0.0 0.0 0.0 76-77 0.05 0.0 0.0 0.0 0.0 78-79 0.075 0.0 0.0 0.0 0.0 80-81 0.1 0.0 0.0 0.0 0.0 82-83 0.1 0.0 0.0 0.0 0.0 84-85 0.175 0.0 0.0 0.0 0.0 86-87 0.25 0.0 0.0 0.0 0.0 88-89 0.36250000000000004 0.0 0.0 0.0 0.0 90-91 0.44999999999999996 0.0 0.0 0.0 0.0 92-93 0.575 0.0 0.0 0.0 0.0 94-95 0.7749999999999999 0.0 0.0 0.0 0.0 96-97 0.9375 0.0 0.0 0.0 0.0 98-99 1.0625 0.0 0.0 0.0 0.0 100-101 1.15 0.0 0.0 0.0 0.0 102-103 1.275 0.0 0.0 0.0 0.0 104-105 1.3624999999999998 0.0 0.0 0.0 0.0 106-107 1.6875 0.0 0.0 0.0 0.0 108-109 1.925 0.0 0.0 0.0 0.0 110-111 2.075 0.0 0.0 0.0 0.0 112-113 2.3375 0.0 0.0 0.0 0.0 114-115 2.5 0.0 0.0 0.0 0.0 116-117 2.8625 0.0 0.0 0.0 0.0 118-119 3.2375 0.0 0.0 0.0 0.0 120-121 3.55 0.0 0.0 0.0 0.0 122-123 4.112500000000001 0.0 0.0 0.0 0.0 124-125 4.55 0.0 0.0 0.0 0.0 126-127 4.925000000000001 0.0 0.0 0.0 0.0 128-129 5.45 0.0 0.0 0.0 0.0 130-131 6.15 0.0 0.0 0.0 0.0 132-133 6.762499999999999 0.0 0.0 0.0 0.0 134-135 7.25 0.0 0.0 0.0 0.0 136-137 7.7625 0.0 0.0 0.0 0.0 138-139 8.4375 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AAGTAAG 10 0.006830828 145.0 6 ATGCTTG 10 0.006830828 145.0 9 AGTAAGG 10 0.006830828 145.0 7 AGTAAAG 10 0.006830828 145.0 2 TCATGCT 10 0.006830828 145.0 7 GTAAGGA 10 0.006830828 145.0 8 CTCATGC 10 0.006830828 145.0 6 TAAAGTA 10 0.006830828 145.0 4 TAAAGCC 10 0.006830828 145.0 145 CATGCTT 10 0.006830828 145.0 8 CATGCTC 10 0.006830828 145.0 2 GTAAAGT 10 0.006830828 145.0 3 TAAGGAG 10 0.006830828 145.0 9 CAGCCTC 10 0.006830828 145.0 145 >>END_MODULE Read 2260318 spots for SRR26075356.sra Written 2260318 spots for SRR26075356.sra Read 2260318 spots for SRR26075356.sra Written 2260318 spots for SRR26075356.sra Read 2260318 spots for SRR26075356.sra Written 2260318 spots for SRR26075356.sra Read 2260318 spots for SRR26075356.sra Written 2260318 spots for SRR26075356.sra Read 2260318 spots for SRR26075356.sra Written 2260318 spots for SRR26075356.sra Read 2260318 spots for SRR26075356.sra Written 2260318 spots for SRR26075356.sra Read 2260318 spots for SRR26075356.sra Written 2260318 spots for SRR26075356.sra Read 2260318 spots for SRR26075356.sra Written 2260318 spots for SRR26075356.sra Read 2260318 spots for SRR26075356.sra Written 2260318 spots for SRR26075356.sra Read 2260318 spots for SRR26075356.sra Written 2260318 spots for SRR26075356.sra Read 2260318 spots for SRR26075356.sra Written 2260318 spots for SRR26075356.sra Read 2260318 spots for SRR26075356.sra Written 2260318 spots for SRR26075356.sra Read 2260327 spots for SRR26075356.sra Written 2260327 spots for SRR26075356.sra Read 2260318 spots for SRR26075356.sra Written 2260318 spots for SRR26075356.sra Read 2260318 spots for SRR26075356.sra Written 2260318 spots for SRR26075356.sra Read 2260318 spots for SRR26075356.sra Written 2260318 spots for SRR26075356.sra Read 2260318 spots for SRR26075356.sra Written 2260318 spots for SRR26075356.sra Read 2260318 spots for SRR26075356.sra Written 2260318 spots for SRR26075356.sra Read 2260318 spots for SRR26075356.sra Written 2260318 spots for SRR26075356.sra Read 2260318 spots for SRR26075356.sra Written 2260318 spots for SRR26075356.sra SRR ids: ['SRR26075356.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_2vpi29s2 SRR26075356.sra spots: 45206369 blocks: [[1, 2260318], [2260319, 4520636], [4520637, 6780954], [6780955, 9041272], [9041273, 11301590], [11301591, 13561908], [13561909, 15822226], [15822227, 18082544], [18082545, 20342862], [20342863, 22603180], [22603181, 24863498], [24863499, 27123816], [27123817, 29384134], [29384135, 31644452], [31644453, 33904770], [33904771, 36165088], [36165089, 38425406], [38425407, 40685724], [40685725, 42946042], [42946043, 45206369]] SRR26075356 file size 16697201 SRR26075356 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o SRR26075356 SRR26075356_1.fastq SRR26075356_2.fastq Input file: SRR26075356_1.fastq Paired file: SRR26075356_2.fastq trimmed: SRR26075356-trimmed-pair1.fastq, SRR26075356-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Tue Feb 11 22:56:42 2025 >> started Tue Feb 11 22:57:39 2025 >> done (57.186s) 45206369 read pairs processed; of these: 169 ( 0.00%) short read pairs filtered out after trimming by size control 59590 ( 0.13%) empty read pairs filtered out after trimming by size control 45146610 (99.87%) read pairs available; of these: 5223210 (11.57%) trimmed read pairs available after processing 39923400 (88.43%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 18 14 0.00% 19 10 0.00% 20 10 0.00% 21 69 0.00% 22 21 0.00% 23 15 0.00% 24 18 0.00% 25 29 0.00% 26 30 0.00% 27 37 0.00% 28 26 0.00% 29 24 0.00% 30 34 0.00% 31 22 0.00% 32 36 0.00% 33 33 0.00% 34 38 0.00% 35 56 0.00% 36 35 0.00% 37 36 0.00% 38 50 0.00% 39 46 0.00% 40 64 0.00% 41 74 0.00% 42 56 0.00% 43 65 0.00% 44 100 0.00% 45 72 0.00% 46 96 0.00% 47 113 0.00% 48 90 0.00% 49 103 0.00% 50 122 0.00% 51 144 0.00% 52 163 0.00% 53 177 0.00% 54 182 0.00% 55 189 0.00% 56 256 0.00% 57 251 0.00% 58 312 0.00% 59 334 0.00% 60 383 0.00% 61 440 0.00% 62 537 0.00% 63 542 0.00% 64 660 0.00% 65 689 0.00% 66 817 0.00% 67 974 0.00% 68 1027 0.00% 69 1245 0.00% 70 1443 0.00% 71 1625 0.00% 72 1968 0.00% 73 2218 0.00% 74 2518 0.01% 75 2823 0.01% 76 3431 0.01% 77 3544 0.01% 78 4085 0.01% 79 4678 0.01% 80 5351 0.01% 81 5670 0.01% 82 6606 0.01% 83 7501 0.02% 84 8724 0.02% 85 9654 0.02% 86 10557 0.02% 87 11294 0.03% 88 12162 0.03% 89 13205 0.03% 90 14655 0.03% 91 15846 0.04% 92 17380 0.04% 93 19192 0.04% 94 20363 0.05% 95 22615 0.05% 96 24462 0.05% 97 25888 0.06% 98 27378 0.06% 99 29126 0.06% 100 30350 0.07% 101 31717 0.07% 102 34375 0.08% 103 36328 0.08% 104 38724 0.09% 105 41475 0.09% 106 44594 0.10% 107 46493 0.10% 108 48608 0.11% 109 50436 0.11% 110 52314 0.12% 111 54032 0.12% 112 57807 0.13% 113 58889 0.13% 114 61649 0.14% 115 65413 0.14% 116 68096 0.15% 117 71155 0.16% 118 75163 0.17% 119 76985 0.17% 120 78495 0.17% 121 81577 0.18% 122 82687 0.18% 123 86754 0.19% 124 89177 0.20% 125 91505 0.20% 126 96047 0.21% 127 99991 0.22% 128 102784 0.23% 129 105061 0.23% 130 109620 0.24% 131 110759 0.25% 132 113674 0.25% 133 116463 0.26% 134 118934 0.26% 135 123138 0.27% 136 125519 0.28% 137 129189 0.29% 138 134351 0.30% 139 137406 0.30% 140 140582 0.31% 141 143530 0.32% 142 147023 0.33% 143 146215 0.32% 144 150709 0.33% 145 155494 0.34% 146 155310 0.34% 147 160568 0.36% 148 163846 0.36% 149 165515 0.37% 150 169756 0.38% 151 39923400 88.43% 45146610 reads passed initial QC criterion=sequence-density sequence-density=0.18 sequence-density-rank=1 fanout-score=7.60 fanout-score-rank=24 prefix-density=0.35 prefix-fanout=4.0 sequence=TCCTTGTCCTGGATCTT criterion=fanout-score sequence-density=0.03 sequence-density-rank=42 fanout-score=164.38 fanout-score-rank=1 prefix-density=0.39 prefix-fanout=14.5 sequence=CATCACCATCCCTGATTGATCTTTGATTCACAACAAGACCAACCAACCGTACAATGTTTACATAACTTGGGATAATCTCACAAGAAAGAAAGATCAATACAAAACTAGTCAGCTCAGGATGTTCCAGGCACGGTAGAACCGTAGAACCATAACAACAAGAGACATATTGCAGATGAGTACTGAAAAACAAAACACAGTACGTATTTACATGGGCAACCTTGGTTGAAGGCAACCTCATCAACGATGCTCGCTCTTCGTCTCTCCACTGTACATCCAGTCATAGACAGTGGGTGTGTTAGGCTGTGGCTTGTCAAAAACATGAGCACCAATATTCTTAGTAGCAAGGTTGCTACCAGGGTGGAACACGCTCCTCCAAACATTGCTACGCGCCGACACTGGGGTTGTAGGGGTCACTGGTGTCGTCGGTGTCCCTGGAGTTCCTGGCATAGTCATGGACCTCTGAAACTTATTAACAGGACTGCTCCCCTCTCCGACGTCAATATCTTTGATG criterion=sequence-density sequence-density=0.26 sequence-density-rank=1 fanout-score=2.49 fanout-score-rank=35 prefix-density=0.26 prefix-fanout=2.5 sequence=ATGTACCCTGACTTAGGTTTCTCAGAGA criterion=fanout-score sequence-density=0.09 sequence-density-rank=22 fanout-score=372.60 fanout-score-rank=1 prefix-density=1.02 prefix-fanout=32.7 sequence=AAGAAGAAGAAA SRR26075356 testing PE reads STAR mapping to Ensembl genome Started job on | Feb 11 22:58:22 Started mapping on | Feb 11 22:58:22 Finished on | Feb 11 23:04:46 Mapping speed, Million of reads per hour | 423.25 Number of input reads | 45146610 Average input read length | 296 UNIQUE READS: Uniquely mapped reads number | 41172415 Uniquely mapped reads % | 91.20% Average mapped length | 295.28 Number of splices: Total | 35233759 Number of splices: Annotated (sjdb) | 34319897 Number of splices: GT/AG | 34596477 Number of splices: GC/AG | 474177 Number of splices: AT/AC | 40243 Number of splices: Non-canonical | 122862 Mismatch rate per base, % | 0.39% Deletion rate per base | 0.03% Deletion average length | 3.01 Insertion rate per base | 0.02% Insertion average length | 2.64 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 1255551 % of reads mapped to multiple loci | 2.78% Number of reads mapped to too many loci | 162333 % of reads mapped to too many loci | 0.36% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 5.35% % of reads unmapped: other | 0.31% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 2718644 2718644 2718644 N_multimapping 1255551 1255551 1255551 N_noFeature 1160648 40695234 1392332 N_ambiguous 510398 2375 263668 UnstrandedReadsAssigned:39501369 PositiveStrandReadsAssigned:474806 NegativeStrandReadsAssigned:39516415 Dataset is classified negative stranded MeadianReadLen=151 20thPercentileLength=151 echo kmer=147 SRR26075356 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,400 [index] number of k-mers: 62,057,036 [index] number of equivalence classes: 130,681 [quant] running in paired-end mode [quant] will process pair 1: SRR26075356-trimmed-pair1.fastq SRR26075356-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 45,146,610 reads, 40,107,200 reads pseudoaligned [quant] estimated average fragment length: 223.687 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,280 rounds 52401 SRR26075356.ke.tsv 34699 SRR26075356.se.tsv 87100 total ==> SRR26075356.ke.tsv <== target_id length eff_length est_counts tpm Potri.005G200100.1.v4.1 2018 1795.31 6583 73.9168 Potri.005G024800.1.v4.1 1035 812.313 4743 117.703 Potri.004G059700.1.v4.1 961 738.313 38 1.03753 Potri.007G009000.2.v4.1 1416 1193.31 0 0 Potri.003G141000.2.v4.1 2943 2720.31 1601 11.864 Potri.016G087400.1.v4.1 270 82.4184 4582 1120.7 Potri.015G069301.1.v4.1 564 343.361 0 0 Potri.010G195200.1.v4.1 1773 1550.31 743 9.66115 Potri.012G127500.1.v4.1 977 754.313 7337 196.077 ==> SRR26075356.se.tsv <== Potri.001G166300.v4.1 0 Potri.001G448400.v4.1 1167 Potri.001G233950.v4.1 0 Potri.001G122700.v4.1 950 Potri.001G212900.v4.1 0 Potri.001G182400.v4.1 2 Potri.001G256600.v4.1 0 Potri.001G040500.v4.1 20 Potri.001G416900.v4.1 0 Potri.001G452600.v4.1 1555 SRR26075356 completed mapping pipeline successfully