Starting /dee2/code/volunteer_pipeline.sh SRR26075357 current disk space = 3048884322304 free memory = 1572588532 SRR26075357 SRAfilesize 2f40f2ab7b4652d7795423d54591896f SRR26075357.sra SRR26075357.sra file validated SRR26075357 is paired end SRR26075357 is conventional basespace SRR26075357 read1 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR26075357_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.61725 37.0 37.0 37.0 37.0 37.0 2 36.6255 37.0 37.0 37.0 37.0 37.0 3 36.733 37.0 37.0 37.0 37.0 37.0 4 36.7 37.0 37.0 37.0 37.0 37.0 5 36.7305 37.0 37.0 37.0 37.0 37.0 6 36.6435 37.0 37.0 37.0 37.0 37.0 7 36.605 37.0 37.0 37.0 37.0 37.0 8 36.6585 37.0 37.0 37.0 37.0 37.0 9 36.647 37.0 37.0 37.0 37.0 37.0 10-14 36.64325 37.0 37.0 37.0 37.0 37.0 15-19 36.6327 37.0 37.0 37.0 37.0 37.0 20-24 36.59779999999999 37.0 37.0 37.0 37.0 37.0 25-29 36.482800000000005 37.0 37.0 37.0 37.0 37.0 30-34 36.4216 37.0 37.0 37.0 37.0 37.0 35-39 36.3959 37.0 37.0 37.0 37.0 37.0 40-44 36.265699999999995 37.0 37.0 37.0 37.0 37.0 45-49 36.0186 37.0 37.0 37.0 37.0 37.0 50-54 36.1461 37.0 37.0 37.0 37.0 37.0 55-59 35.755700000000004 37.0 37.0 37.0 37.0 37.0 60-64 35.7251 37.0 37.0 37.0 37.0 37.0 65-69 35.6487 37.0 37.0 37.0 37.0 37.0 70-74 35.92360000000001 37.0 37.0 37.0 37.0 37.0 75-79 36.0883 37.0 37.0 37.0 37.0 37.0 80-84 36.089299999999994 37.0 37.0 37.0 37.0 37.0 85-89 35.9567 37.0 37.0 37.0 37.0 37.0 90-94 35.986900000000006 37.0 37.0 37.0 37.0 37.0 95-99 35.995599999999996 37.0 37.0 37.0 37.0 37.0 100-104 35.89829999999999 37.0 37.0 37.0 37.0 37.0 105-109 35.8074 37.0 37.0 37.0 37.0 37.0 110-114 35.6783 37.0 37.0 37.0 37.0 37.0 115-119 35.6529 37.0 37.0 37.0 37.0 37.0 120-124 35.660700000000006 37.0 37.0 37.0 37.0 37.0 125-129 35.536500000000004 37.0 37.0 37.0 37.0 37.0 130-134 35.3682 37.0 37.0 37.0 37.0 37.0 135-139 35.285399999999996 37.0 37.0 37.0 29.8 37.0 140-144 35.225100000000005 37.0 37.0 37.0 29.8 37.0 145-149 35.1635 37.0 37.0 37.0 25.0 37.0 150-151 34.940749999999994 37.0 37.0 37.0 25.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.0 1101 2 0.0 1101 3 0.0 1101 4 0.0 1101 5 0.0 1101 6 0.0 1101 7 0.0 1101 8 0.0 1101 9 0.0 1101 10-14 0.0 1101 15-19 0.0 1101 20-24 0.0 1101 25-29 0.0 1101 30-34 0.0 1101 35-39 0.0 1101 40-44 0.0 1101 45-49 0.0 1101 50-54 0.0 1101 55-59 0.0 1101 60-64 0.0 1101 65-69 0.0 1101 70-74 0.0 1101 75-79 0.0 1101 80-84 0.0 1101 85-89 0.0 1101 90-94 0.0 1101 95-99 0.0 1101 100-104 0.0 1101 105-109 0.0 1101 110-114 0.0 1101 115-119 0.0 1101 120-124 0.0 1101 125-129 0.0 1101 130-134 0.0 1101 135-139 0.0 1101 140-144 0.0 1101 145-149 0.0 1101 150-151 0.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 21 1.0 22 1.0 23 4.0 24 10.0 25 9.0 26 7.0 27 4.0 28 13.0 29 20.0 30 42.0 31 35.0 32 53.0 33 132.0 34 243.0 35 450.0 36 2755.0 37 221.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 31.971936857930345 12.227511901779001 11.5760461037334 44.224505136557255 2 17.724999999999998 16.25 34.9 31.125000000000004 3 17.8 16.175 28.175 37.85 4 22.375 22.375 22.925 32.324999999999996 5 26.1 29.95 24.575 19.375 6 25.224999999999998 32.275 22.925 19.575 7 15.225 30.75 36.95 17.075000000000003 8 18.6 28.975 30.049999999999997 22.375 9 19.8 24.775 35.449999999999996 19.975 10-14 20.051002550127507 29.741487074353717 26.88134406720336 23.326166308315415 15-19 18.9 27.455000000000002 28.315 25.330000000000002 20-24 20.080000000000002 28.125 27.73 24.065 25-29 19.685 28.494999999999997 28.015 23.805 30-34 19.56 27.505000000000003 27.935 25.0 35-39 21.36 26.83 27.67 24.14 40-44 19.25 28.15 27.82 24.779999999999998 45-49 20.19 27.61 28.560000000000002 23.64 50-54 20.575 26.265 28.4 24.759999999999998 55-59 19.575 27.0 29.299999999999997 24.125 60-64 21.02 26.765 28.26 23.955000000000002 65-69 20.705000000000002 27.689999999999998 28.389999999999997 23.215 70-74 21.91 26.97 27.215 23.905 75-79 21.84 27.765 27.189999999999998 23.205000000000002 80-84 23.169999999999998 25.89 27.939999999999998 23.0 85-89 22.3 26.295 27.800000000000004 23.605 90-94 22.98 27.235 26.995 22.79 95-99 22.66 26.424999999999997 27.095000000000002 23.82 100-104 23.585 27.11 27.015 22.29 105-109 22.395 27.034999999999997 26.985 23.585 110-114 22.07 28.13 26.44 23.36 115-119 22.74 26.545 27.150000000000002 23.565 120-124 22.814999999999998 27.279999999999998 26.83 23.075000000000003 125-129 21.709999999999997 27.67 26.365 24.255 130-134 22.470000000000002 27.445000000000004 26.365 23.72 135-139 23.35 27.245 25.795 23.61 140-144 23.425 26.365 26.58 23.630000000000003 145-149 23.87 26.43 26.634999999999998 23.064999999999998 150-151 23.4625 26.237500000000004 25.775 24.525 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.5 19 0.5 20 0.5 21 1.0 22 0.5 23 1.0 24 1.0 25 1.5 26 4.5 27 6.5 28 4.5 29 4.5 30 8.0 31 13.0 32 29.0 33 40.5 34 41.0 35 44.5 36 62.0 37 107.5 38 144.0 39 154.5 40 151.0 41 189.0 42 231.0 43 263.0 44 291.0 45 279.5 46 289.0 47 263.5 48 225.0 49 213.0 50 182.0 51 142.5 52 110.5 53 98.5 54 76.5 55 42.5 56 35.0 57 29.5 58 17.0 59 23.0 60 22.0 61 10.5 62 9.0 63 10.0 64 9.0 65 12.0 66 26.0 67 29.0 68 18.5 69 13.5 70 7.5 71 3.5 72 3.0 73 1.5 74 0.5 75 0.5 76 0.5 77 0.0 78 0.0 79 0.5 80 0.5 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.22499999999999998 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.005 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150-151 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 59.699999999999996 #Duplication Level Percentage of deduplicated Percentage of total 1 63.484087102177554 37.9 2 21.02177554438861 25.1 3 8.710217755443885 15.6 4 3.8107202680067003 9.1 5 1.7587939698492463 5.25 6 0.5862646566164154 2.1 7 0.37688442211055273 1.575 8 0.04187604690117253 0.2 9 0.0 0.0 >10 0.16750418760469013 1.6 >50 0.04187604690117253 1.575 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGTGGTACATCTCGTAT 63 1.575 TruSeq Adapter, Index 13 (97% over 36bp) GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGTGGTACATCGCGTAT 27 0.675 TruSeq Adapter, Index 13 (97% over 36bp) CACCTCCAGCAATGTATTCTACATTGTCCTTGCTAGCCATTTCATCGTAC 14 0.35000000000000003 No Hit CCACCATAACCTCCCTTAGTAAGAATTATGTCTAGCAGCTCTGCCCCGGC 12 0.3 No Hit CCCTAACAATCTTACATCAAATTACAAGCACGTATGGTCTTGTAATATTT 11 0.27499999999999997 No Hit CCCAAAATCATCCCGCACCCCCAAGCAAAGCACCCAGCAAAACCCTCATT 8 0.2 No Hit GTCCAGTCTTGTATTTTCACCATTTCAATAGCAATGACTAAGCTATTTAC 7 0.17500000000000002 No Hit ATGGACAAAAGTAGATCAACAAGCTAGAAAATGCTTAGTCACCTCCTACC 7 0.17500000000000002 No Hit GAAGACCCACAAGAGAACCATGGCAGTCATAAGACCACCTCTGTTGGAAG 7 0.17500000000000002 No Hit GGGGAGAGAATATTATCTGAATTACATTGTAAAAGAAAAGTCCCCATGAT 7 0.17500000000000002 No Hit CCCCAATTATGAGCACTTTCTCATCAGAATCATAAGCTCTTTCTTCTTCT 7 0.17500000000000002 No Hit GTGTTTTGTAGAAAACAGATGCTCGCTTCCTGTCCAAACCAGTCATGCAC 7 0.17500000000000002 No Hit CCTTCACAATCAAGCTGCAACGACAGGATATGATGCACAGGTTGCAATAC 7 0.17500000000000002 No Hit CCCCAGACTCGGATAACACCATCGGTATAGCCACTGAACAAGGTGCTTCC 7 0.17500000000000002 No Hit CTCGTTCTTGAGATATGTGGTATCAAAGAAAATGACATCACCGAAATTTC 7 0.17500000000000002 No Hit GTTGTGGAGGATATGCTGAAGTTGCCGGATACGGTGCAGGAGGATAAGTT 6 0.15 No Hit CCTTCTTCTGTCCCTCCCCAGAATCACATAACCTACTCTTCATTGCTTCA 6 0.15 No Hit GCCAGAGGCATCAGGATCAGACAAAGATGATGTCTTCCTGCTCTTCTGCT 6 0.15 No Hit TTTTAAACTATTCTAGAAACACCAGGAGACACATTATCCAGTACACGCTT 6 0.15 No Hit GTTGAGGTTGCATAGGTTGCTGTTCCACTTAAGCGACTAGCTATTGATTT 6 0.15 No Hit GTGCAGTGTAGTCTCCTCACACTCGTCCAGCTCCATATCTGTCAACTGGC 6 0.15 No Hit CCCGGTTGATGATATACCTTTGACGTATTCTTGATACAAGATTGCTCTTC 6 0.15 No Hit TGTGGAAATCAGCAGTTGGGTCAAACCAAAGATAGAATTGCTGCTCTCTG 6 0.15 No Hit CGGCAATATATCTCACAAAATCTGTCATGCTTCCTTCACTGAACCTGCTC 6 0.15 No Hit GCGCAGTCTGGGGACTTCATCTGCTGTTGCACCGAAGAGAAAGAAATTCT 6 0.15 No Hit CCAGGCAAAAGAAAAGGTGCACACAACAATCTCACACAGGGACATGAATA 6 0.15 No Hit AGAGGCAGTATGCGTTCCATGACCATCACCATCAAATGGAGAAGCATAGT 6 0.15 No Hit CACGTATCATCATCTGGGAATAAAACTCAGCATGTTCCTTAACTGTGACG 6 0.15 No Hit CTTAATCTCACATAACTCTTACTGTAATTAATAACAATCAATCAAAAACA 6 0.15 No Hit ATCTTTTCGTAGCTCAACTCTTTAGGTAGACCCTGAACTGTAGTCAAGCT 5 0.125 No Hit CTCATGAGATCTTCCTGACCAAACTGTTCATTAAAGAGGGTAGTTTGAAG 5 0.125 No Hit CTTGGCTGCAGACTTCTGGTTCTTTCGCTTCACTCTACCAGGACGTCCAC 5 0.125 No Hit CTCTCAGCAAGTAATGATATTGATGATGGAAGGAGGGAATGGAGGGATTG 5 0.125 No Hit GTACTTGCTTGTCTCGCCTTAATCGATTCACTTCCCCATCCAGTCCAAAT 5 0.125 No Hit GACATTTATACCAAGACTCGGAAACAAATTGGGGTCATTATAGAGTTTGC 5 0.125 No Hit CGCTCTCCATCAAACCTCCCAATTTCCCCCATTGGGTATTGCCCAAAACC 5 0.125 No Hit CTGTGAATCCTAGCAGCAAATGTGTTTGGATCATCTAGGCTGAAGCCAGA 5 0.125 No Hit ATAGGTGATGGCGATGAATTTCCTCTAAAACCTCAAAAAGTGCATCTGTC 5 0.125 No Hit TGCAAATACTGATAATACAGGGTGACAAAATCAGTAGACCTGTTAACTAC 5 0.125 No Hit GTCATATTTATAAAGACTTCATTTGCATAAGCTCCATAAACTTCAGAAGA 5 0.125 No Hit GTTCGCTCTTCGTCTCTCCACTGTACATCCAGTCATAGACAGTGGGTGTG 5 0.125 No Hit CTTGGCTGGATCTTGCTGGTGGGCTCAGGCATCTCCAAAGTCTTTGTTTG 5 0.125 No Hit CGGGCCTCTTCCTACCACCCCGCCTGACACGGATACGATAAACCACGTAG 5 0.125 No Hit GTTGGCTCCACACTTGCAGCCATTCTCGGCTCCCACGACCGTCTCAGCAG 5 0.125 No Hit CGCATCATGACAGCAAACTCGTGGAAGCTAAGAACACCGTCTCCATTGAG 5 0.125 No Hit CTCAGGCTTTACATCTCCATGCACATAGCCTCTAGAGTGCATCTTCTCTA 5 0.125 No Hit TGATCACTAAAATTAAACTCAAACTCCGGCATCAAATCCCTCATCGTTTC 5 0.125 No Hit CTCCTTTTTCCAAGCCTCTTCTTCTCTTGCTTCTTCTTCCCTGTGTGTAT 5 0.125 No Hit GTCTCGCTTGTAGGCCCTCCATGGCTTTTCAACAATAATGGAGGCTTTTC 5 0.125 No Hit GTTCTTTCATAATTTTGATAGTAGCGAATAACTTTCGCTTGTCCAAGAAT 5 0.125 No Hit TGGCACATCAGCGTAACTAGTAACATGAACCAGCCGCCCTCAACTTCTTC 5 0.125 No Hit GATCATAGAGGGTGTTGAAGAAAAATGGAGTTGACGGTGAGAGAATGTTC 5 0.125 No Hit CCGGGAACAATTTGATGCTGAAAAAACCTTTCTTTTTCCACTCTTAGAAC 5 0.125 No Hit CATGTTGGTGCCTCCTTGCATCATCATTATCATGATCATCTCTACTTCTT 5 0.125 No Hit CCACCTGATTCAACAGCTTATTCATAGCTTCATCACTCTTAGCCGCATGA 5 0.125 No Hit CTCATACTAATTTAGGAATGGGCCAGCAAGCCAAGCATGTAAAAGAACTT 5 0.125 No Hit GGCGGGGATTGGGAGATTTGTAGAACCGCGAGCTAGCATGACAAGACAGA 5 0.125 No Hit ACAATTTGAATGTACTTCCTTGATGGATTTTCCAGCATGGTCTCCGAGCT 5 0.125 No Hit CTTGCAGGAATCACAATTGTAGCATTTGGTAGCCCGGGAATTTTTGTAAA 5 0.125 No Hit CACCAACTCAGGCCTCATCACTCCCATCGACGCCACACCAACTCCACTCT 5 0.125 No Hit CTCCTGTCTTCTCGACTCCACAAGGAGGGCGCGCTGCTCTGGACTTTTTT 5 0.125 No Hit CCGTACTTTTCTATCCCGAACTGCTCGGCCAGGACTTTCCATAAATGCTT 5 0.125 No Hit CTCCAAGCATGGCATTCTTCCAGTCATGAGTGCCACGAATTCTCTCCATT 5 0.125 No Hit CTCAGAATTCAATCTTTTAACAGCAATTACAGTTCCACTTCCATTTCTCC 5 0.125 No Hit CACGTCTACCCTCGATAAAGATCATTGGGATATGAGAAATCTTGGGCCAC 5 0.125 No Hit CTTGTCAATGCTGCTTGGCAAGCAGTCCATCAAGCACTGGGCTCCTGAAA 5 0.125 No Hit ACATCAATATAACGCCATACCAAGTCACGATTCATGCCCCCACTTCCACA 5 0.125 No Hit CCGGAGACTCTGTCCTGTGAGAGGAGCATGAGGCAGGAAGATTTGTGCGT 5 0.125 No Hit CCAAATGAAATGGTGCACCCGACAAGCCCCAACACAGCCAAGAATCCATA 5 0.125 No Hit CTGTATTAAACGCTCTTCAAGTTCAGCATCATTTGCACCCACTGGCAAAT 5 0.125 No Hit TGTACTGATTCCTGCTAGCTGTTGTCCACTGTCCTCCATCCATACCAACA 5 0.125 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0125 0.0 0.0 0.0 0.0 64-65 0.0625 0.0 0.0 0.0 0.0 66-67 0.075 0.0 0.0 0.0 0.0 68-69 0.075 0.0 0.0 0.0 0.0 70-71 0.0875 0.0 0.0 0.0 0.0 72-73 0.125 0.0 0.0 0.0 0.0 74-75 0.125 0.0 0.0 0.0 0.0 76-77 0.125 0.0 0.0 0.0 0.0 78-79 0.175 0.0 0.0 0.0 0.0 80-81 0.2 0.0 0.0 0.0 0.0 82-83 0.2625 0.0 0.0 0.0 0.0 84-85 0.4625 0.0 0.0 0.0 0.0 86-87 0.5375 0.0 0.0 0.0 0.0 88-89 0.6875 0.0 0.0 0.0 0.0 90-91 0.775 0.0 0.0 0.0 0.0 92-93 0.9125 0.0 0.0 0.0 0.0 94-95 1.2 0.0 0.0 0.0 0.0 96-97 1.3625 0.0 0.0 0.0 0.0 98-99 1.4874999999999998 0.0 0.0 0.0 0.0 100-101 1.7999999999999998 0.0 0.0 0.0 0.0 102-103 2.15 0.0 0.0 0.0 0.0 104-105 2.6625 0.0 0.0 0.0 0.0 106-107 3.1125 0.0 0.0 0.0 0.0 108-109 3.3375 0.0 0.0 0.0 0.0 110-111 3.675 0.0 0.0 0.0 0.0 112-113 4.2 0.0 0.0 0.0 0.0 114-115 4.7 0.0 0.0 0.0 0.0 116-117 4.95 0.0 0.0 0.0 0.0 118-119 5.237500000000001 0.0 0.0 0.0 0.0 120-121 5.574999999999999 0.0 0.0 0.0 0.0 122-123 6.112500000000001 0.0 0.0 0.0 0.0 124-125 6.4125 0.0 0.0 0.0 0.0 126-127 6.8375 0.0 0.0 0.0 0.0 128-129 7.45 0.0 0.0 0.0 0.0 130-131 7.8375 0.0 0.0 0.0 0.0 132-133 8.2375 0.0 0.0 0.0 0.0 134-135 8.9375 0.0 0.0 0.0 0.0 136-137 9.475 0.0 0.0 0.0 0.0 138-139 10.325 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CCAGATT 10 0.006830828 145.0 5 GATTTCT 10 0.006830828 145.0 8 ATATTCC 10 0.006830828 145.0 145 ACCAGAT 10 0.006830828 145.0 4 >>END_MODULE SRR26075357 read2 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR26075357_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.2875 37.0 37.0 37.0 37.0 37.0 2 36.2485 37.0 37.0 37.0 37.0 37.0 3 36.2885 37.0 37.0 37.0 37.0 37.0 4 36.285 37.0 37.0 37.0 37.0 37.0 5 36.3675 37.0 37.0 37.0 37.0 37.0 6 36.295 37.0 37.0 37.0 37.0 37.0 7 36.309 37.0 37.0 37.0 37.0 37.0 8 36.257 37.0 37.0 37.0 37.0 37.0 9 36.283 37.0 37.0 37.0 37.0 37.0 10-14 36.1241 37.0 37.0 37.0 37.0 37.0 15-19 36.0252 37.0 37.0 37.0 37.0 37.0 20-24 35.9169 37.0 37.0 37.0 37.0 37.0 25-29 35.7641 37.0 37.0 37.0 37.0 37.0 30-34 35.5552 37.0 37.0 37.0 37.0 37.0 35-39 35.4293 37.0 37.0 37.0 37.0 37.0 40-44 35.4298 37.0 37.0 37.0 37.0 37.0 45-49 35.2468 37.0 37.0 37.0 37.0 37.0 50-54 35.05650000000001 37.0 37.0 37.0 37.0 37.0 55-59 35.1248 37.0 37.0 37.0 34.6 37.0 60-64 35.3067 37.0 37.0 37.0 37.0 37.0 65-69 35.1646 37.0 37.0 37.0 32.2 37.0 70-74 34.93580000000001 37.0 37.0 37.0 25.0 37.0 75-79 34.8711 37.0 37.0 37.0 25.0 37.0 80-84 34.9889 37.0 37.0 37.0 29.8 37.0 85-89 35.05400000000001 37.0 37.0 37.0 25.0 37.0 90-94 35.020799999999994 37.0 37.0 37.0 25.0 37.0 95-99 35.2341 37.0 37.0 37.0 34.6 37.0 100-104 35.214000000000006 37.0 37.0 37.0 34.6 37.0 105-109 35.2094 37.0 37.0 37.0 34.6 37.0 110-114 35.119099999999996 37.0 37.0 37.0 29.8 37.0 115-119 35.133799999999994 37.0 37.0 37.0 29.8 37.0 120-124 35.072 37.0 37.0 37.0 27.4 37.0 125-129 35.0622 37.0 37.0 37.0 25.0 37.0 130-134 35.0021 37.0 37.0 37.0 27.4 37.0 135-139 34.83969999999999 37.0 37.0 37.0 25.0 37.0 140-144 34.92524999999999 37.0 37.0 37.0 25.0 37.0 145-149 34.850199999999994 37.0 37.0 37.0 25.0 37.0 150-151 34.56375 37.0 37.0 37.0 25.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.0 1101 2 0.0 1101 3 0.0 1101 4 0.0 1101 5 0.0 1101 6 0.0 1101 7 0.0 1101 8 0.0 1101 9 0.0 1101 10-14 0.0 1101 15-19 0.0 1101 20-24 0.0 1101 25-29 0.0 1101 30-34 0.0 1101 35-39 0.0 1101 40-44 0.0 1101 45-49 0.0 1101 50-54 0.0 1101 55-59 0.0 1101 60-64 0.0 1101 65-69 0.0 1101 70-74 0.0 1101 75-79 0.0 1101 80-84 0.0 1101 85-89 0.0 1101 90-94 0.0 1101 95-99 0.0 1101 100-104 0.0 1101 105-109 0.0 1101 110-114 0.0 1101 115-119 0.0 1101 120-124 0.0 1101 125-129 0.0 1101 130-134 0.0 1101 135-139 0.0 1101 140-144 0.0 1101 145-149 0.0 1101 150-151 0.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 12 1.0 13 9.0 14 15.0 15 10.0 16 9.0 17 5.0 18 4.0 19 7.0 20 8.0 21 11.0 22 5.0 23 10.0 24 21.0 25 24.0 26 33.0 27 37.0 28 28.0 29 27.0 30 17.0 31 33.0 32 53.0 33 84.0 34 210.0 35 660.0 36 2473.0 37 206.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 35.65 17.925 18.675 27.750000000000004 2 30.925000000000004 23.25 28.4 17.424999999999997 3 24.025 27.875 28.425 19.675 4 27.150000000000002 31.125000000000004 22.25 19.475 5 30.4 32.725 20.424999999999997 16.45 6 24.349999999999998 34.875 22.325 18.45 7 23.325000000000003 20.025000000000002 37.974999999999994 18.675 8 27.0 25.2 24.85 22.95 9 24.625 26.724999999999998 26.900000000000002 21.75 10-14 26.584999999999997 29.005 24.75 19.66 15-19 26.665 27.529999999999998 25.86 19.945 20-24 26.424999999999997 28.12 25.014999999999997 20.44 25-29 27.04 27.694999999999997 25.224999999999998 20.04 30-34 27.425 26.695 26.314999999999998 19.564999999999998 35-39 26.619999999999997 27.555000000000003 26.185000000000002 19.64 40-44 27.150000000000002 27.455000000000002 26.275 19.12 45-49 26.77 27.105 26.625 19.5 50-54 23.990000000000002 27.91 28.875 19.225 55-59 25.674999999999997 26.669999999999998 27.639999999999997 20.015 60-64 27.005000000000003 27.744999999999997 26.165 19.085 65-69 27.29 26.224999999999998 26.88 19.605 70-74 24.695 28.49 26.135 20.68 75-79 25.09 29.110000000000003 26.66 19.139999999999997 80-84 26.945000000000004 27.534999999999997 26.615 18.905 85-89 27.389999999999997 26.939999999999998 26.534999999999997 19.134999999999998 90-94 27.005000000000003 28.199999999999996 26.240000000000002 18.555 95-99 26.985 27.57 26.340000000000003 19.105 100-104 28.09 26.974999999999998 26.265 18.67 105-109 26.845000000000002 27.935 26.295 18.925 110-114 26.88 27.860000000000003 25.485000000000003 19.775000000000002 115-119 27.915 26.615 26.43 19.040000000000003 120-124 27.42 27.725 25.790000000000003 19.064999999999998 125-129 28.375 28.23 25.019999999999996 18.375 130-134 28.599999999999998 27.26 25.775 18.365000000000002 135-139 27.87 27.644999999999996 26.575 17.91 140-144 29.069360404060607 27.784167625143773 25.598839825973897 17.547632144821723 145-149 29.048714614384313 26.823046914074222 25.84775432629789 18.280484145243573 150-151 30.182545636409102 27.19429857464366 25.618904726181547 17.00425106276569 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.5 3 1.5 4 1.0 5 0.0 6 0.0 7 0.5 8 0.5 9 0.5 10 1.0 11 0.5 12 1.0 13 2.5 14 2.5 15 1.0 16 0.5 17 1.0 18 0.5 19 0.5 20 1.0 21 1.5 22 1.0 23 0.0 24 1.0 25 2.0 26 2.0 27 5.0 28 7.0 29 5.5 30 7.5 31 15.5 32 22.5 33 36.5 34 50.5 35 48.0 36 77.0 37 99.5 38 113.0 39 150.0 40 191.0 41 221.0 42 242.0 43 262.0 44 275.5 45 278.5 46 262.5 47 257.0 48 240.5 49 201.0 50 179.0 51 132.5 52 74.0 53 65.0 54 59.5 55 49.5 56 37.5 57 30.0 58 27.0 59 21.5 60 16.5 61 7.0 62 5.5 63 7.5 64 4.5 65 2.5 66 1.5 67 2.0 68 3.0 69 3.0 70 2.5 71 1.5 72 2.0 73 2.5 74 2.0 75 1.0 76 1.5 77 2.5 78 1.5 79 2.0 80 5.5 81 5.5 82 7.0 83 8.5 84 7.5 85 6.0 86 6.0 87 10.0 88 10.5 89 8.0 90 8.0 91 7.0 92 7.0 93 7.5 94 4.0 95 4.5 96 6.0 97 2.5 98 0.5 99 1.5 100 19.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.015 145-149 0.03 150-151 0.025 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 60.575 #Duplication Level Percentage of deduplicated Percentage of total 1 64.63062319438713 39.15 2 20.42921997523731 24.75 3 8.2955014444903 15.075 4 3.8794882377218323 9.4 5 1.650846058605035 5.0 6 0.6190672719768882 2.25 7 0.2888980602558811 1.225 8 0.04127115146512588 0.2 9 0.0 0.0 >10 0.12381345439537762 1.35 >50 0.04127115146512588 1.6 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG 64 1.6 No Hit GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGT 29 0.7250000000000001 No Hit CACATAAGCTCCTCCATTTCTCTCTTTCGATTTCTCTCTCTCTATCTCTA 13 0.325 No Hit GGAAAGGGAGAGGATGATGGTGGTGGTGGAGTCTGTGGTTTGTCCGAAGC 12 0.3 No Hit TGAGAAATTACCACCTCCAGAGCCTAAGAAGCCTAAGACTATCGAGGTCA 8 0.2 No Hit GTCTCTTTGTATACCCTTACAAAGTTATCATCAAAACTTGTGGGACTACT 7 0.17500000000000002 No Hit GCGGAGCGCGTCATCTGGACATGTTAGAAGTTCGTTCTCAAGCAGTGCTG 7 0.17500000000000002 No Hit TAAGATTTGGGATCTGGAGAGCAAGATGGTTGTTGATGATTTGAAGGTTG 7 0.17500000000000002 No Hit GTTGCGTTCGAGGTAGTAGGTAGTTTCCGTCTGTACAAGGAAGGAGTTTA 7 0.17500000000000002 No Hit CAACCACAACCATCAGCACTACTACTACAGCCCAACAATAACAAAAGGAA 7 0.17500000000000002 No Hit GTGTTTCAATTTTATTGACGAAGCCAAGAGGCAGGGAGGTGGTGTTTTGG 7 0.17500000000000002 No Hit TGAAGGAGATGCTCAAGCTATGCTTGATCATTTTATGCATATGCAAGATG 7 0.17500000000000002 No Hit GAAAGATTGACATGCAACTCAAGCTTGTCCCTGGCAACTCTGCAGGCACT 6 0.15 No Hit GAGATGTATTCAGGGCACCAATCAACTCTGGTTTACTTTGTGTGTATGCA 6 0.15 No Hit CTCTGATTAAAAATATAAAGGAATAAAAGTGGAAGACAGAGAAGAAAGGA 6 0.15 No Hit CGATGAGGGTATGCCGATGTACCAAAGGCCATTCATGAAAGGGAAGTTGT 6 0.15 No Hit GCTAATTGATGATAGCCGCCATTCTGAAAGCTTAGTTGCAAAGGTATTAC 6 0.15 No Hit ATTTGGTGACAGTGCTAAGGCAACACAAAGGACTGTGAGTAACCCGTTTC 6 0.15 No Hit GCAGGCTATGCTCCTTCAGCACCACAATATGGAGCCCCTGTTCCTCAAGT 6 0.15 No Hit ATCGTCGTGGGGCATTGCTGATGAGGGGCTGGGGAGGCTGATTTAGCTGT 6 0.15 No Hit GGAGGAAGAAGAACGAGTCATCAGATGAAGAGGATGATCGGCAACGCCGT 6 0.15 No Hit ACAGAGTTCTATCTTTGGCATTACTTAATGTAACTGGTGGAGGTTTCGAA 6 0.15 No Hit GCAAAGCAAAGCCAAGAATCTTGAAATATTTACCAAATCTTTGTGTTTCC 6 0.15 No Hit GCAAGGAGCCTTCTGTCTGCTCAGGGGATCCATTTCTACTTCTTGCTATT 6 0.15 No Hit GAGATGCTTATTCCTGGAAGTGAGTTGTCACAGCATATCAGCACAGCAGG 6 0.15 No Hit AGTGGGTCCACCACAGGAGAAACGAAGCGATGCTAAAAAGGTTACAAGTA 6 0.15 No Hit GACATATCTTTGAACTCATATCCAGTCCCTAGCCATTTTTCAGGCATTTG 6 0.15 No Hit GAGAGATCCAAGAAATTTTCAACTCCAAGAATCTCTGTTCAGTTCAGATC 5 0.125 No Hit AAGCATTCTAATAAAAGCACATCAAAATACGGTAAACGTGTCCGGAGAAG 5 0.125 No Hit AAACAGAGAGAGATAAAACAAAACAAAACAAAACAAAGGGTTGGCTTATC 5 0.125 No Hit AGAGGTAACAACAGCTTTATTATTTGGGATCCCCAAGCCTTTTCCACGAG 5 0.125 No Hit TGCAAGCACGAGGGAGCTCCGTTGATGTTTCCAGGTACTAAAGAGGCGTG 5 0.125 No Hit AACAGGACCACCATCTACTTATGCTGATCGGGTGTTTGAAAATGAAATAA 5 0.125 No Hit CCAAAAACCCTAAAAGCTCAAACCTTTGGCGGCTATCCACTGAACCCACC 5 0.125 No Hit ATTAAACTCAAAGCATCTTTTCTATGAAGAAATGTGTTCCTATCACAATG 5 0.125 No Hit CACTGACATCAGTATGTACTGATACACGGAGGAAGCAAAAAGAGCGGTGG 5 0.125 No Hit AAAACAACAGCTGCTCTCTCTTTCTCTTAAGTTAGCAATTCACCCTCCTT 5 0.125 No Hit TGAATCTGAAGGAAAATGGGGTGTGCCTCCACCTGCTGAGAATGGGTCAG 5 0.125 No Hit CTTCTTCGTCCTCCCTACATCCCATCATTTCCTTCACGCCCTCACTTCTA 5 0.125 No Hit CAACTTTCACCCAGAGGTTCCGAACTGACGAAGTGCATGCAATTTGGAGA 5 0.125 No Hit GCTCCTCTCCGAGGATTGAGGATTTCCCGAGGAACGCAAGAGCAAAACAT 5 0.125 No Hit GATGAACAAGGTTGGGAAGCTTCCTTGTGCCCAGCTGGCCTTCATCGTAA 5 0.125 No Hit CATGAGTTCCACCTCCAGTGCATTCTTGAATGGTGTCAAAGGAGTTCCCA 5 0.125 No Hit CGAAGAGGATGGTGGTTTCGGGGTCTGGTAAAGAGTGTTCACACGTGTAT 5 0.125 No Hit GACGGTTTTGATTATGAGCTTTATAATAGGAATGATATTAACCGGATTCT 5 0.125 No Hit CCCCTATTCTCTTCGTGAAGATGTCTTGCTGTGGAGGAAACTGTGGGTGT 5 0.125 No Hit TAAAAACCCTAGTCACCAGCCAGGCACCGCTGCTGCTCTGATCCAATGGG 5 0.125 No Hit TGGAGACGGGCTTTTGGGGTTCCAGGACTTCCTATGCTTGATGACAGGCA 5 0.125 No Hit GTTTGAGGAAGCATTGCCAGAATGGTTGGGCAACCTTTCTTCTCTTCAAT 5 0.125 No Hit AAACCTTACAGAAACTTCTCGCTCAGATAATGTGACTGCTATCCATCAGG 5 0.125 No Hit GGACAGGGCAGGAATATATCTGCTGTCAGCAAGCCAATATTTTCAGCAGG 5 0.125 No Hit CGCCTCCAGACACTTGTTTTCAAGGCTGGTATGGCTAAGTCGATCCACCA 5 0.125 No Hit TTAACAAAAGCACAAGAATCGTCTACATCTTGTGCAACTAAGTTAACAGC 5 0.125 No Hit AAGCTAGGCAAGATGGTTTTACTAGACAAGATGTGGGATGATGTTGTTGC 5 0.125 No Hit GCGATATCAAATATATCCCCAGTGCATCTTTTAATCTGATTCTAATCAAT 5 0.125 No Hit TTTGCTGATGGTGTTAGGAGGGATAAGGCATCCTTGTGTTTGTGCTGCCC 5 0.125 No Hit GAAGGCTCAGGCATTGAGGGACAGCAGCATGAGCTCGTACATGTCAAGCA 5 0.125 No Hit TGATGACTGCAATAACCTACCAAGAAAATATAAGACTATTAAGCAGTACA 5 0.125 No Hit GAGAAACAAGAAAGAATCAATATGCCAAGTAGCAGTTTCGGGGCTTCTTT 5 0.125 No Hit CACAAGGGAGCCCAAAGAAACCAAGAAGCCATGATTTGATACAATCCCCA 5 0.125 No Hit AGTCAAAGCAAAAAGATTGCTGACGCTAAATTAGCAAAAGATTTTCAAGC 5 0.125 No Hit GACACTCCCATGAAGCTCGCAGACTACTTCAAAATTCAGGGAGTTTTCAA 5 0.125 No Hit CGTTGCTTATATGTTGAGGGAGCATCTGCAATTCATCCATCATCAGTGGC 5 0.125 No Hit CTAATGAGAAGCTGAAGAAGACATTTGCTTGTTACCTCTCAACATCTACT 5 0.125 No Hit TGGATATCCAAATCAGCAGACGGGACAGGGTGGTTCTGGGAGAGGACAAC 5 0.125 No Hit AAAGCATAATCAGCTGCATAATTCTAAAAGGGTGCCGCAGCAATTTGAGG 5 0.125 No Hit AAACTGTGGGTGTGGCTCTGGCTGCAAGTGCGGCAGCGGCTGTGGAGGAT 5 0.125 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0125 0.0 0.0 0.0 0.0 64-65 0.05 0.0 0.0 0.0 0.0 66-67 0.05 0.0 0.0 0.0 0.0 68-69 0.05 0.0 0.0 0.0 0.0 70-71 0.0625 0.0 0.0 0.0 0.0 72-73 0.075 0.0 0.0 0.0 0.0 74-75 0.075 0.0 0.0 0.0 0.0 76-77 0.075 0.0 0.0 0.0 0.0 78-79 0.125 0.0 0.0 0.0 0.0 80-81 0.15 0.0 0.0 0.0 0.0 82-83 0.21250000000000002 0.0 0.0 0.0 0.0 84-85 0.4125 0.0 0.0 0.0 0.0 86-87 0.48750000000000004 0.0 0.0 0.0 0.0 88-89 0.6125 0.0 0.0 0.0 0.0 90-91 0.675 0.0 0.0 0.0 0.0 92-93 0.8125 0.0 0.0 0.0 0.0 94-95 1.1 0.0 0.0 0.0 0.0 96-97 1.2625 0.0 0.0 0.0 0.0 98-99 1.3875000000000002 0.0 0.0 0.0 0.0 100-101 1.7000000000000002 0.0 0.0 0.0 0.0 102-103 2.0374999999999996 0.0 0.0 0.0 0.0 104-105 2.5625 0.0 0.0 0.0 0.0 106-107 3.0375 0.0 0.0 0.0 0.0 108-109 3.2625 0.0 0.0 0.0 0.0 110-111 3.5999999999999996 0.0 0.0 0.0 0.0 112-113 4.125 0.0 0.0 0.0 0.0 114-115 4.625 0.0 0.0 0.0 0.0 116-117 4.875 0.0 0.0 0.0 0.0 118-119 5.175 0.0 0.0 0.0 0.0 120-121 5.525 0.0 0.0 0.0 0.0 122-123 6.0625 0.0 0.0 0.0 0.0 124-125 6.4125 0.0 0.0 0.0 0.0 126-127 6.8625 0.0 0.0 0.0 0.0 128-129 7.425 0.0 0.0 0.0 0.0 130-131 7.775 0.0 0.0 0.0 0.0 132-133 8.149999999999999 0.0 0.0 0.0 0.0 134-135 8.8625 0.0 0.0 0.0 0.0 136-137 9.375 0.0 0.0 0.0 0.0 138-139 10.1875 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CACACCT 10 0.006830828 145.0 145 ATAAGAC 10 0.006830828 145.0 3 TAAGACA 10 0.006830828 145.0 4 GACAACT 10 0.006830828 145.0 7 CAAACCT 10 0.006830828 145.0 9 ACAACTG 10 0.006830828 145.0 8 AAGACAA 30 0.0017973486 72.5 5 >>END_MODULE Read 1903933 spots for SRR26075357.sra Written 1903933 spots for SRR26075357.sra Read 1903933 spots for SRR26075357.sra Written 1903933 spots for SRR26075357.sra Read 1903933 spots for SRR26075357.sra Written 1903933 spots for SRR26075357.sra Read 1903933 spots for SRR26075357.sra Written 1903933 spots for SRR26075357.sra Read 1903933 spots for SRR26075357.sra Written 1903933 spots for SRR26075357.sra Read 1903933 spots for SRR26075357.sra Written 1903933 spots for SRR26075357.sra Read 1903933 spots for SRR26075357.sra Written 1903933 spots for SRR26075357.sra Read 1903933 spots for SRR26075357.sra Written 1903933 spots for SRR26075357.sra Read 1903933 spots for SRR26075357.sra Written 1903933 spots for SRR26075357.sra Read 1903933 spots for SRR26075357.sra Written 1903933 spots for SRR26075357.sra Read 1903933 spots for SRR26075357.sra Written 1903933 spots for SRR26075357.sra Read 1903933 spots for SRR26075357.sra Written 1903933 spots for SRR26075357.sra Read 1903933 spots for SRR26075357.sra Written 1903933 spots for SRR26075357.sra Read 1903933 spots for SRR26075357.sra Written 1903933 spots for SRR26075357.sra Read 1903951 spots for SRR26075357.sra Written 1903951 spots for SRR26075357.sra Read 1903933 spots for SRR26075357.sra Written 1903933 spots for SRR26075357.sra Read 1903933 spots for SRR26075357.sra Written 1903933 spots for SRR26075357.sra Read 1903933 spots for SRR26075357.sra Written 1903933 spots for SRR26075357.sra Read 1903933 spots for SRR26075357.sra Written 1903933 spots for SRR26075357.sra Read 1903933 spots for SRR26075357.sra Written 1903933 spots for SRR26075357.sra SRR ids: ['SRR26075357.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_zpfn0ax9 SRR26075357.sra spots: 38078678 blocks: [[1, 1903933], [1903934, 3807866], [3807867, 5711799], [5711800, 7615732], [7615733, 9519665], [9519666, 11423598], [11423599, 13327531], [13327532, 15231464], [15231465, 17135397], [17135398, 19039330], [19039331, 20943263], [20943264, 22847196], [22847197, 24751129], [24751130, 26655062], [26655063, 28558995], [28558996, 30462928], [30462929, 32366861], [32366862, 34270794], [34270795, 36174727], [36174728, 38078678]] SRR26075357 file size 14062849 SRR26075357 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o SRR26075357 SRR26075357_1.fastq SRR26075357_2.fastq Input file: SRR26075357_1.fastq Paired file: SRR26075357_2.fastq trimmed: SRR26075357-trimmed-pair1.fastq, SRR26075357-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Wed Feb 12 03:17:49 2025 >> started Wed Feb 12 03:18:33 2025 >> done (44.054s) 38078678 read pairs processed; of these: 308 ( 0.00%) short read pairs filtered out after trimming by size control 891452 ( 2.34%) empty read pairs filtered out after trimming by size control 37186918 (97.66%) read pairs available; of these: 5705502 (15.34%) trimmed read pairs available after processing 31481416 (84.66%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 18 8 0.00% 19 11 0.00% 20 15 0.00% 21 19 0.00% 22 14 0.00% 23 15 0.00% 24 22 0.00% 25 32 0.00% 26 38 0.00% 27 43 0.00% 28 45 0.00% 29 49 0.00% 30 45 0.00% 31 66 0.00% 32 56 0.00% 33 64 0.00% 34 66 0.00% 35 64 0.00% 36 113 0.00% 37 158 0.00% 38 598 0.00% 39 173 0.00% 40 362 0.00% 41 198 0.00% 42 214 0.00% 43 234 0.00% 44 268 0.00% 45 212 0.00% 46 273 0.00% 47 296 0.00% 48 360 0.00% 49 419 0.00% 50 547 0.00% 51 555 0.00% 52 703 0.00% 53 698 0.00% 54 772 0.00% 55 972 0.00% 56 859 0.00% 57 916 0.00% 58 1462 0.00% 59 1327 0.00% 60 1755 0.00% 61 1779 0.00% 62 2082 0.01% 63 2662 0.01% 64 2555 0.01% 65 2709 0.01% 66 2865 0.01% 67 3367 0.01% 68 3713 0.01% 69 4163 0.01% 70 4839 0.01% 71 5201 0.01% 72 6335 0.02% 73 7145 0.02% 74 7919 0.02% 75 8529 0.02% 76 9339 0.03% 77 10233 0.03% 78 10845 0.03% 79 11834 0.03% 80 13478 0.04% 81 14407 0.04% 82 16285 0.04% 83 17981 0.05% 84 20056 0.05% 85 21512 0.06% 86 22988 0.06% 87 24471 0.07% 88 25484 0.07% 89 27046 0.07% 90 28434 0.08% 91 30491 0.08% 92 32965 0.09% 93 35059 0.09% 94 37729 0.10% 95 40147 0.11% 96 42576 0.11% 97 43824 0.12% 98 44176 0.12% 99 45921 0.12% 100 47598 0.13% 101 49246 0.13% 102 52017 0.14% 103 54303 0.15% 104 57100 0.15% 105 59130 0.16% 106 62398 0.17% 107 63406 0.17% 108 63379 0.17% 109 65604 0.18% 110 66833 0.18% 111 68784 0.18% 112 70851 0.19% 113 72895 0.20% 114 74888 0.20% 115 78333 0.21% 116 80539 0.22% 117 82945 0.22% 118 85751 0.23% 119 86644 0.23% 120 87165 0.23% 121 89306 0.24% 122 89871 0.24% 123 92009 0.25% 124 96060 0.26% 125 98716 0.27% 126 100414 0.27% 127 104819 0.28% 128 106462 0.29% 129 109246 0.29% 130 110198 0.30% 131 109201 0.29% 132 110721 0.30% 133 113176 0.30% 134 114997 0.31% 135 117373 0.32% 136 121314 0.33% 137 121691 0.33% 138 126446 0.34% 139 127575 0.34% 140 129590 0.35% 141 129682 0.35% 142 131531 0.35% 143 131963 0.35% 144 133171 0.36% 145 136411 0.37% 146 137765 0.37% 147 141142 0.38% 148 143295 0.39% 149 146180 0.39% 150 147108 0.40% 151 31481416 84.66% 37186918 reads passed initial QC criterion=sequence-density sequence-density=0.36 sequence-density-rank=1 fanout-score=17.14 fanout-score-rank=5 prefix-density=0.30 prefix-fanout=17.1 sequence=GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGTGGTACATCTCGTATGCCGTCTTCTGCTTGAAAAT criterion=fanout-score sequence-density=0.06 sequence-density-rank=39 fanout-score=78.41 fanout-score-rank=1 prefix-density=0.36 prefix-fanout=12.6 sequence=CAACAGCAACACAAGGCAGCACAACATCCCTTCCAGAAGCCATCACCCCTGGATTTAGTCTTCACAGGAATAGGATTTTGCTGGTCGGATTGACCATCCATGGTTGGATAGCCAGCTGGTGGTGGGCCTGAATATGCACTAGGATAAGTTGATGTTGGTGGGGGAGGATATGCCACTGTAGCTTGATTCTGACTCATGGTTGAAGAAGCGAGTTGAGAAGCACGAACAAATGAGAGCTATGGAAGAATGGAGATAAGGACGAGAGAAGTTCGGTTGTGGT criterion=sequence-density sequence-density=0.33 sequence-density-rank=1 fanout-score=2.90 fanout-score-rank=33 prefix-density=0.33 prefix-fanout=2.9 sequence=ATGTACCCTGACTTAGGTTTCTCAGA criterion=fanout-score sequence-density=0.01 sequence-density-rank=38 fanout-score=549.34 fanout-score-rank=1 prefix-density=0.42 prefix-fanout=17.6 sequence=GAGAAGAAACACTAAAAAAATCACACAAGCGAAATCTCCAGCGAGAAGAGAAAGATGGATTTCAGAATCATGGGTCTTGACGCGCCACTCTTCAACACCCTCCAGCACATGATGGATGCAAGTGATCATGAGGCAGACAAGTCCTTCAATGCGCCAACACGCACTTACGTACGTGATGCCAAGGCAATGGCATCAACACCAGCTGATGTGAAAGAGTATCCAAGCTCTTATGCGTTCGTCATTGACATGCCGGGACTGAAATCAGGGGACATCAAGGTTCAAGTGGA SRR26075357 testing PE reads STAR mapping to Ensembl genome Started job on | Feb 12 03:19:13 Started mapping on | Feb 12 03:19:14 Finished on | Feb 12 03:24:09 Mapping speed, Million of reads per hour | 453.81 Number of input reads | 37186918 Average input read length | 293 UNIQUE READS: Uniquely mapped reads number | 33578865 Uniquely mapped reads % | 90.30% Average mapped length | 291.98 Number of splices: Total | 27528624 Number of splices: Annotated (sjdb) | 26716842 Number of splices: GT/AG | 27047668 Number of splices: GC/AG | 356741 Number of splices: AT/AC | 31309 Number of splices: Non-canonical | 92906 Mismatch rate per base, % | 0.44% Deletion rate per base | 0.03% Deletion average length | 2.95 Insertion rate per base | 0.02% Insertion average length | 2.63 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 995144 % of reads mapped to multiple loci | 2.68% Number of reads mapped to too many loci | 133101 % of reads mapped to too many loci | 0.36% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 5.92% % of reads unmapped: other | 0.75% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 2612909 2612909 2612909 N_multimapping 995144 995144 995144 N_noFeature 918029 33109331 1250157 N_ambiguous 354959 2608 216259 UnstrandedReadsAssigned:32305877 PositiveStrandReadsAssigned:466926 NegativeStrandReadsAssigned:32112449 Dataset is classified negative stranded MeadianReadLen=151 20thPercentileLength=151 echo kmer=147 SRR26075357 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,400 [index] number of k-mers: 62,057,036 [index] number of equivalence classes: 130,681 [quant] running in paired-end mode [quant] will process pair 1: SRR26075357-trimmed-pair1.fastq SRR26075357-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 37,186,918 reads, 32,704,657 reads pseudoaligned [quant] estimated average fragment length: 216.296 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,052 rounds 52401 SRR26075357.ke.tsv 34699 SRR26075357.se.tsv 87100 total ==> SRR26075357.ke.tsv <== target_id length eff_length est_counts tpm Potri.005G200100.1.v4.1 2018 1802.7 5637.29 82.7426 Potri.005G024800.1.v4.1 1035 819.704 14808 477.994 Potri.004G059700.1.v4.1 961 745.76 5 0.1774 Potri.007G009000.2.v4.1 1416 1200.7 0 0 Potri.003G141000.2.v4.1 2943 2727.7 1576.35 15.2911 Potri.016G087400.1.v4.1 270 91.2097 2469.41 716.366 Potri.015G069301.1.v4.1 564 351.431 0 0 Potri.010G195200.1.v4.1 1773 1557.7 273 4.63725 Potri.012G127500.1.v4.1 977 761.76 14873 516.611 ==> SRR26075357.se.tsv <== Potri.001G166300.v4.1 0 Potri.001G448400.v4.1 11 Potri.001G233950.v4.1 0 Potri.001G122700.v4.1 282 Potri.001G212900.v4.1 0 Potri.001G182400.v4.1 0 Potri.001G256600.v4.1 0 Potri.001G040500.v4.1 21 Potri.001G416900.v4.1 0 Potri.001G452600.v4.1 879 SRR26075357 completed mapping pipeline successfully