Starting /dee2/code/volunteer_pipeline.sh SRR26075358 current disk space = 3048955568128 free memory = 1575229176 SRR26075358 SRAfilesize c2fdf1ef9d366dd64c4ef75f5f629d34 SRR26075358.sra SRR26075358.sra file validated SRR26075358 is paired end SRR26075358 is conventional basespace SRR26075358 read1 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR26075358_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.534 37.0 37.0 37.0 37.0 37.0 2 36.561 37.0 37.0 37.0 37.0 37.0 3 36.616 37.0 37.0 37.0 37.0 37.0 4 36.699 37.0 37.0 37.0 37.0 37.0 5 36.5905 37.0 37.0 37.0 37.0 37.0 6 36.667 37.0 37.0 37.0 37.0 37.0 7 36.5775 37.0 37.0 37.0 37.0 37.0 8 36.6455 37.0 37.0 37.0 37.0 37.0 9 36.598 37.0 37.0 37.0 37.0 37.0 10-14 36.60305 37.0 37.0 37.0 37.0 37.0 15-19 36.6002 37.0 37.0 37.0 37.0 37.0 20-24 36.5124 37.0 37.0 37.0 37.0 37.0 25-29 36.400400000000005 37.0 37.0 37.0 37.0 37.0 30-34 36.387299999999996 37.0 37.0 37.0 37.0 37.0 35-39 36.3161 37.0 37.0 37.0 37.0 37.0 40-44 36.2493 37.0 37.0 37.0 37.0 37.0 45-49 36.016000000000005 37.0 37.0 37.0 37.0 37.0 50-54 35.994 37.0 37.0 37.0 37.0 37.0 55-59 35.9303 37.0 37.0 37.0 37.0 37.0 60-64 35.8435 37.0 37.0 37.0 37.0 37.0 65-69 35.750299999999996 37.0 37.0 37.0 37.0 37.0 70-74 35.8919 37.0 37.0 37.0 37.0 37.0 75-79 36.0033 37.0 37.0 37.0 37.0 37.0 80-84 35.9894 37.0 37.0 37.0 37.0 37.0 85-89 35.8853 37.0 37.0 37.0 37.0 37.0 90-94 35.8412 37.0 37.0 37.0 37.0 37.0 95-99 35.8567 37.0 37.0 37.0 37.0 37.0 100-104 35.825700000000005 37.0 37.0 37.0 37.0 37.0 105-109 35.744299999999996 37.0 37.0 37.0 37.0 37.0 110-114 35.585899999999995 37.0 37.0 37.0 37.0 37.0 115-119 35.4984 37.0 37.0 37.0 37.0 37.0 120-124 35.476099999999995 37.0 37.0 37.0 37.0 37.0 125-129 35.458999999999996 37.0 37.0 37.0 37.0 37.0 130-134 35.3316 37.0 37.0 37.0 32.2 37.0 135-139 35.2013 37.0 37.0 37.0 29.8 37.0 140-144 35.0614 37.0 37.0 37.0 27.4 37.0 145-149 35.1286 37.0 37.0 37.0 27.4 37.0 150-151 34.92 37.0 37.0 37.0 25.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.0 1101 2 0.0 1101 3 0.0 1101 4 0.0 1101 5 0.0 1101 6 0.0 1101 7 0.0 1101 8 0.0 1101 9 0.0 1101 10-14 0.0 1101 15-19 0.0 1101 20-24 0.0 1101 25-29 0.0 1101 30-34 0.0 1101 35-39 0.0 1101 40-44 0.0 1101 45-49 0.0 1101 50-54 0.0 1101 55-59 0.0 1101 60-64 0.0 1101 65-69 0.0 1101 70-74 0.0 1101 75-79 0.0 1101 80-84 0.0 1101 85-89 0.0 1101 90-94 0.0 1101 95-99 0.0 1101 100-104 0.0 1101 105-109 0.0 1101 110-114 0.0 1101 115-119 0.0 1101 120-124 0.0 1101 125-129 0.0 1101 130-134 0.0 1101 135-139 0.0 1101 140-144 0.0 1101 145-149 0.0 1101 150-151 0.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 17 1.0 18 0.0 19 0.0 20 1.0 21 4.0 22 6.0 23 4.0 24 5.0 25 2.0 26 12.0 27 13.0 28 20.0 29 23.0 30 38.0 31 49.0 32 82.0 33 128.0 34 164.0 35 424.0 36 2855.0 37 169.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 41.578947368421055 15.538847117794486 7.769423558897243 35.11278195488722 2 19.875 14.6 32.550000000000004 32.975 3 16.7 18.45 30.725 34.125 4 20.45 24.099999999999998 26.150000000000002 29.299999999999997 5 23.849999999999998 29.9 24.975 21.275 6 23.775 32.025 21.975 22.225 7 16.1 30.099999999999998 37.775 16.025 8 19.075 28.15 31.275 21.5 9 19.225 24.675 34.050000000000004 22.05 10-14 20.116005800290015 29.5864793239662 27.806390319515977 22.491124556227813 15-19 20.119999999999997 29.054999999999996 27.63 23.195 20-24 19.905 28.125 28.365000000000002 23.605 25-29 19.825 27.834999999999997 27.875 24.465 30-34 20.16 29.34 27.29 23.21 35-39 20.635 27.66 27.644999999999996 24.060000000000002 40-44 20.135 29.04 27.355 23.47 45-49 20.985 27.994999999999997 27.500000000000004 23.52 50-54 21.634999999999998 28.075 26.825 23.465 55-59 20.925 27.505000000000003 28.095 23.474999999999998 60-64 20.62 27.91 28.465 23.005 65-69 20.919999999999998 27.644999999999996 27.85 23.585 70-74 21.584999999999997 27.1 27.68 23.635 75-79 21.615000000000002 26.855 27.16 24.37 80-84 21.57 27.72 27.825 22.884999999999998 85-89 21.990000000000002 26.93 27.43 23.65 90-94 22.189999999999998 28.09 26.21 23.51 95-99 21.77 27.505000000000003 27.36 23.365 100-104 21.240000000000002 27.639999999999997 27.045 24.075 105-109 22.39 27.41 26.6 23.599999999999998 110-114 21.67 27.61 26.405 24.315 115-119 22.38 27.61 26.21 23.799999999999997 120-124 21.584999999999997 26.150000000000002 27.839999999999996 24.425 125-129 23.145 27.315 25.97 23.57 130-134 22.505 26.640000000000004 27.08 23.775 135-139 23.255 27.505000000000003 26.135 23.105 140-144 22.32 26.840000000000003 26.165 24.675 145-149 23.09 26.875 26.14 23.895 150-151 23.1875 27.55 25.137500000000003 24.125 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 0.0 1 0.5 2 0.5 3 1.5 4 1.5 5 0.0 6 0.0 7 0.5 8 1.0 9 0.5 10 0.5 11 0.5 12 0.5 13 0.5 14 0.0 15 0.5 16 1.5 17 2.0 18 1.0 19 0.0 20 1.5 21 1.5 22 0.0 23 2.5 24 4.0 25 10.0 26 12.5 27 9.0 28 11.5 29 14.0 30 17.5 31 28.5 32 33.0 33 39.0 34 55.5 35 63.0 36 84.0 37 110.5 38 111.0 39 134.5 40 172.0 41 182.5 42 206.5 43 229.5 44 231.5 45 238.0 46 280.0 47 293.5 48 242.0 49 193.5 50 154.0 51 145.0 52 145.0 53 113.0 54 78.5 55 65.5 56 58.0 57 40.0 58 26.0 59 22.5 60 19.0 61 13.0 62 8.5 63 7.0 64 6.0 65 3.0 66 1.0 67 6.0 68 13.0 69 15.5 70 11.0 71 5.0 72 7.0 73 6.5 74 3.0 75 1.0 76 0.0 77 0.5 78 0.5 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.5 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.25 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.005 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150-151 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 60.525 #Duplication Level Percentage of deduplicated Percentage of total 1 63.320941759603464 38.324999999999996 2 21.10698058653449 25.55 3 8.880627839735647 16.125 4 3.593556381660471 8.7 5 1.6935150764147047 5.125 6 0.7434944237918215 2.7 7 0.24783147459727387 1.05 8 0.12391573729863693 0.6 9 0.08261049153242463 0.44999999999999996 >10 0.20652622883106153 1.375 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GATCGGAAGAGCACACGTCTGAACTCCAGTCACACACCAGTATCTCGTTT 13 0.325 TruSeq Adapter, Index 23 (97% over 37bp) CTTGCATCAAATTGGTCATATCTACATGACAGAACAAAATACACAGGCAG 11 0.27499999999999997 No Hit GATCGGAAGAGCACACGTCTGAACTCCAGTCACACACCAGTATCTCGGTT 11 0.27499999999999997 TruSeq Adapter, Index 23 (97% over 37bp) CTCCGAAGCGGTAGCATTTGATGTTAACAGGATAAGGTCCCAGCTCCACG 10 0.25 No Hit GGAAGCATAAGAAAGAGTGCCATTGCCCCAGCTATGGTGCAAAAGTTTTG 10 0.25 No Hit CCCATCCGCAGATCATAGTACCCATACTCAAACCCATCCCCTTATATGAA 9 0.22499999999999998 No Hit CGGGCTTCATTTCAGTAGAATTTGCCCGTGCAGCAGCTTTTACAAGAGTT 9 0.22499999999999998 No Hit TGGCCCTTGTGAATTCATCACCTTGTCAAAAGCAGATAGAAAAGCCAGTC 8 0.2 No Hit CAGAAGGTTCCTGCCTGGCTGGAAGCTTCTTAAGTGCTTCAGCCTCTTCC 8 0.2 No Hit GCACTCAAAAGCTGCTGTCACAATAAGCTCCACATAAAATAGTGGCAAGG 8 0.2 No Hit GCAGGAAGCAAGAAAACAACAAAGTTGTTGTTAAGGATTTATCCATGATT 7 0.17500000000000002 No Hit CGCATCCAATCTAAGCTCTCCTCCAGCTCCTTTGTCTGAATTTCTGATCC 7 0.17500000000000002 No Hit TACTTGGAGTGGAAATAACAAGCAACAGAGAAATAGCACTTGGAAGAAGG 7 0.17500000000000002 No Hit GCTTTAACTGATAAATTCGACCTTCATGTACAGTATTGTGAGGACCAATT 7 0.17500000000000002 No Hit TCGTTATAGCTCTCCTGATGCCTCTAACAACAGCTAGATAAGGGTCACAG 7 0.17500000000000002 No Hit CCGATGCCTTCTCAACCTATGCTATGGAAATTCAGATAGAACTTGTCCCC 7 0.17500000000000002 No Hit GCTGCCTTTCGATCTTGGTACACCTAATGTTTCATTTTCAGCATAGTAGA 6 0.15 No Hit CCCTTGCTAGGCAATGCTGCTCCACACTAATTCGCTCCTTCTGGTTTCTC 6 0.15 No Hit GAACTCATCTTCAGAACTGATAATGATCTCACACTAGTATAAAAGAAGAA 6 0.15 No Hit CCAGAGTAATAGCATATAAACCAAGACATTATCTTGGACAACATGAACAG 6 0.15 No Hit CGTCAACAACCTGGGAAGGCCATACAGAATGAGAATTGCAGTTACTTCAC 6 0.15 No Hit GCTTCTTTTGCTGAGATTTCAATTCCTTTACCCTCTCTTCAGTAGCCTTC 6 0.15 No Hit CTTCACAATATCAGTCCATGGAGGCAACTCTACCTTGCCAGATCGTTTAA 6 0.15 No Hit CTTTTCCTATATATGTAGCGGCGTCAGCCCTGTTCCGTTTTTGTGTGACG 6 0.15 No Hit TTCCCCTCCTTAACCAGAACAAAGCTTTTCAAGAGCCGAGTTTTCATCAC 6 0.15 No Hit CCTGAAGGGTAATTAATTTACCGGCGAGTAGAGTAGAGTTTTTCAAGGTG 6 0.15 No Hit CATGGATTTACTCGAGTTTCACACTGGAATGTTTTGAACTTACAATGCGA 6 0.15 No Hit GCCCTTGAAGCTGCTGCTTCCCTCACTGAAGTTCAGTGACCTCCTGGACT 6 0.15 No Hit TTTTTTTTGGATTTTTTCCGCTTTGATATTCTCTGCATCCTATTTAGGGC 6 0.15 No Hit GCTGTAATTATACATAAAGAAAACTGATGTATAGCCTAGAAATCAAAATT 6 0.15 No Hit GATCGGAAGAGCACACGTCTGAACTCCAGTCACACACCAGTATCGCGTTT 6 0.15 TruSeq Adapter, Index 23 (97% over 37bp) ATCATCATTTGCAAATTGGCCTTCCAAGCAAGAATCTGACAGCATTTGAA 6 0.15 No Hit GCAGCTGTGAGGGCAAGGATGGTGACCGGAAGAAAGGACAGGTGGTCTGA 6 0.15 No Hit GATCGGAAGAGCACACGTCTGAACTCCAGTCACACACCAGTATCGCGGTT 6 0.15 TruSeq Adapter, Index 23 (97% over 37bp) ATCCACTCAGGGTGTCCAAACTCATTTCCCATAAAATTCAAATATCCTTC 5 0.125 No Hit TGCAACTTCTGGTTCTGGTGCCTGTGTCTCACCAGACTCGGTAGCCTCTT 5 0.125 No Hit CAGTAGACAATCCAACTGAAAGGTCTGCAATGAAAGTATCCTCAGTTTCA 5 0.125 No Hit GCTTGAGGATGGTAAAGCAGTGGCCTATGTGTCACTGCATTGATATGTTG 5 0.125 No Hit CCCAATTAAGCCAAGTTTACACGATATGTTTTTGGAATGAGGCACAGTAA 5 0.125 No Hit GGATAAAATGTCTGTAGCACTTTGTATTGATTACTCACCGAACTGAATCC 5 0.125 No Hit ATGGGAGGTGTGTCATGTCTCTTGATGTCAAACAGTGGTCTTCCCTTCGA 5 0.125 No Hit GCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGA 5 0.125 No Hit ATCGGCAAGACCGAGGCTAAGCACCTCAGACACGACCTTGAGATCACAGT 5 0.125 No Hit TGTTACTATGAGATCATTGTGAATGATTGCAGTGTAGGGAAATTGACAAA 5 0.125 No Hit CTCCGTCTCAAGGTCAAACATTCTATTCACAGCATGCATAATCTCAAGCA 5 0.125 No Hit GTTTGCTCAGAATGAATGCCTCTGGGCAATACCATGTTATGTAGTGAGGG 5 0.125 No Hit GAACCGGATCTTGAAAACACAATGGGATTAGCTGGCTGCCGCTGCTGGAC 5 0.125 No Hit TCGTTTACATGTAAAGCTAACTGCTAACTATGGCACTACGTACTAATCTA 5 0.125 No Hit GTCAACAAATCTGCGGCGCTCCTCAAGTCCTGGGTATTTATTATAGTCCA 5 0.125 No Hit GCATAGGGGGGATTTTTTGGATGGTGGCTAGAACTGTAAATTGGTTTCAT 5 0.125 No Hit CGTGTATTTGTGTTATTTCTCCCACTAACAATTTCCAAAAGGAGGACACC 5 0.125 No Hit AGTGACACCACCAGAACCCTTCTTTCCTCGGATAACAGCTCCAGGTTCGC 5 0.125 No Hit ATCCTCGTGCCCAAGTTAAGAAGCCCCAGAAGCTGCAGCTGCAACGTACA 5 0.125 No Hit GGGTCGAACACAGGATCACAGATATGAGATTGAATTTAAGATGGTCCCAA 5 0.125 No Hit ATAAGATAGAAAAGGAGGCATTCAGGGATAAAACTAGAACTAATAAAGCA 5 0.125 No Hit GGTTGAAGTGTATAGAATTTTAGTACTTGCTAGTAAGGAGGAGGTGGTGG 5 0.125 No Hit CAATGCTGCTCAAATTATCCTCATTTTTAGCATTTCTATGGAATACAGAG 5 0.125 No Hit CGCTAAAGTTACAATCTGTGGGGTCTTTAGCAACTTTCCAAGAGAGTATT 5 0.125 No Hit GCTTATAATACATAACTGACTAATTTTCATTCACATCTACATGAAAATCA 5 0.125 No Hit GCAAGTCTCTTGCTTCGGTGGTGTTGGTGGTGGCTTCACGATTGGTGGTG 5 0.125 No Hit CCTCCCTGCACCTCGACGGCTGCACATGCTCCACCCTAACATGAATTTTC 5 0.125 No Hit CCCCAGTTACCACGGCCAGACCCTTCGCGTTTAAGCTCATTCCCACGACC 5 0.125 No Hit CCTCTCTATTCCTCTCAAAGTCAATCCCTTGTCGCCTCAGCAGCTCAATC 5 0.125 No Hit GGTGAACTGTGTGTAACCCTTCCGCTCGAGTATCTGAGCTGCACCTTTGA 5 0.125 No Hit GTCTGGATGTCGACTGTGTTGTCGTGGCAGCCTTTAGGGCCCCAAAGAAG 5 0.125 No Hit GAGAACATGATCGCAAGCAAATTTACCCAACTATAATCCTATGAGCTACC 5 0.125 No Hit ATTGGGATCGACACCAGGCCATTCACGGCAGAGACAGTGTTGCCCCTCAG 5 0.125 No Hit TGATGAGGAAGATGAGGAAGCTGGAGCTGATGCTGCAAAAGTTGTGAGAG 5 0.125 No Hit GATCTTTCAGCTGTCGCAATGTGTCCCATATTTTTTGCAAGATTATCTAT 5 0.125 No Hit CTTCAACATTCTCCACACCAACTCCTCCAGCAATTGCTAATAAGCAGTTT 5 0.125 No Hit GTTTTATGGATAATTGGGATATTCTTTGGTATAGTTGGGATTTTAATATC 5 0.125 No Hit CGGTGATCTTCAAGGCCCGGGTAAATGACATTGAGGCCTAGTTTTTTTAT 5 0.125 No Hit GGCTCCAGCAACGAATCTATACTGCACTTCGATCCACTGGGCACAAGCTC 5 0.125 No Hit CGGAAGGAACGGAAGCCAACTTTTCCCTGCCGGAAGCAATCAGCTCAGTG 5 0.125 No Hit CTGCAATCATTTCCACAGGTCTCGCAACAAAAATTGCCTTTTGAAGGTTG 5 0.125 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.025 0.0 0.0 0.0 0.0 64-65 0.1 0.0 0.0 0.0 0.0 66-67 0.1 0.0 0.0 0.0 0.0 68-69 0.1125 0.0 0.0 0.0 0.0 70-71 0.1375 0.0 0.0 0.0 0.0 72-73 0.15 0.0 0.0 0.0 0.0 74-75 0.275 0.0 0.0 0.0 0.0 76-77 0.275 0.0 0.0 0.0 0.0 78-79 0.3125 0.0 0.0 0.0 0.0 80-81 0.3625 0.0 0.0 0.0 0.0 82-83 0.375 0.0 0.0 0.0 0.0 84-85 0.3875 0.0 0.0 0.0 0.0 86-87 0.475 0.0 0.0 0.0 0.0 88-89 0.55 0.0 0.0 0.0 0.0 90-91 0.5874999999999999 0.0 0.0 0.0 0.0 92-93 0.65 0.0 0.0 0.0 0.0 94-95 0.7375 0.0 0.0 0.0 0.0 96-97 0.8999999999999999 0.0 0.0 0.0 0.0 98-99 1.0750000000000002 0.0 0.0 0.0 0.0 100-101 1.2125 0.0 0.0 0.0 0.0 102-103 1.3624999999999998 0.0 0.0 0.0 0.0 104-105 1.7125 0.0 0.0 0.0 0.0 106-107 2.075 0.0 0.0 0.0 0.0 108-109 2.1625 0.0 0.0 0.0 0.0 110-111 2.325 0.0 0.0 0.0 0.0 112-113 2.55 0.0 0.0 0.0 0.0 114-115 3.1875 0.0 0.0 0.0 0.0 116-117 3.5250000000000004 0.0 0.0 0.0 0.0 118-119 4.2 0.0 0.0 0.0 0.0 120-121 4.6875 0.0 0.0 0.0 0.0 122-123 5.175000000000001 0.0 0.0 0.0 0.0 124-125 5.65 0.0 0.0 0.0 0.0 126-127 6.25 0.0 0.0 0.0 0.0 128-129 6.8625 0.0 0.0 0.0 0.0 130-131 7.3625 0.0 0.0 0.0 0.0 132-133 7.85 0.0 0.0 0.0 0.0 134-135 8.15 0.0 0.0 0.0 0.0 136-137 8.9875 0.0 0.0 0.0 0.0 138-139 9.65 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TTCAAGC 10 0.006830828 145.0 7 >>END_MODULE SRR26075358 read2 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR26075358_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.0445 37.0 37.0 37.0 37.0 37.0 2 36.0355 37.0 37.0 37.0 37.0 37.0 3 36.0765 37.0 37.0 37.0 37.0 37.0 4 36.1765 37.0 37.0 37.0 37.0 37.0 5 36.007 37.0 37.0 37.0 37.0 37.0 6 36.1405 37.0 37.0 37.0 37.0 37.0 7 35.9835 37.0 37.0 37.0 37.0 37.0 8 36.054 37.0 37.0 37.0 37.0 37.0 9 36.1465 37.0 37.0 37.0 37.0 37.0 10-14 36.0118 37.0 37.0 37.0 37.0 37.0 15-19 35.9295 37.0 37.0 37.0 37.0 37.0 20-24 35.8515 37.0 37.0 37.0 37.0 37.0 25-29 35.6402 37.0 37.0 37.0 37.0 37.0 30-34 35.4745 37.0 37.0 37.0 37.0 37.0 35-39 35.4395 37.0 37.0 37.0 37.0 37.0 40-44 35.3618 37.0 37.0 37.0 37.0 37.0 45-49 35.2247 37.0 37.0 37.0 37.0 37.0 50-54 35.1356 37.0 37.0 37.0 37.0 37.0 55-59 35.111 37.0 37.0 37.0 34.6 37.0 60-64 35.0729 37.0 37.0 37.0 29.8 37.0 65-69 35.08919999999999 37.0 37.0 37.0 29.8 37.0 70-74 34.9622 37.0 37.0 37.0 25.0 37.0 75-79 34.8572 37.0 37.0 37.0 25.0 37.0 80-84 34.944900000000004 37.0 37.0 37.0 27.4 37.0 85-89 34.8567 37.0 37.0 37.0 25.0 37.0 90-94 34.9116 37.0 37.0 37.0 25.0 37.0 95-99 34.998599999999996 37.0 37.0 37.0 25.0 37.0 100-104 34.8529 37.0 37.0 37.0 25.0 37.0 105-109 34.9288 37.0 37.0 37.0 25.0 37.0 110-114 34.7645 37.0 37.0 37.0 25.0 37.0 115-119 34.8307 37.0 37.0 37.0 25.0 37.0 120-124 34.64059999999999 37.0 37.0 37.0 25.0 37.0 125-129 34.6395 37.0 37.0 37.0 25.0 37.0 130-134 34.552099999999996 37.0 37.0 37.0 25.0 37.0 135-139 34.477799999999995 37.0 37.0 37.0 25.0 37.0 140-144 34.4504 37.0 37.0 37.0 25.0 37.0 145-149 34.4673 37.0 37.0 37.0 25.0 37.0 150-151 34.10724999999999 37.0 37.0 37.0 25.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.0 1101 2 0.0 1101 3 0.0 1101 4 0.0 1101 5 0.0 1101 6 0.0 1101 7 0.0 1101 8 0.0 1101 9 0.0 1101 10-14 0.0 1101 15-19 0.0 1101 20-24 0.0 1101 25-29 0.0 1101 30-34 0.0 1101 35-39 0.0 1101 40-44 0.0 1101 45-49 0.0 1101 50-54 0.0 1101 55-59 0.0 1101 60-64 0.0 1101 65-69 0.0 1101 70-74 0.0 1101 75-79 0.0 1101 80-84 0.0 1101 85-89 0.0 1101 90-94 0.0 1101 95-99 0.0 1101 100-104 0.0 1101 105-109 0.0 1101 110-114 0.0 1101 115-119 0.0 1101 120-124 0.0 1101 125-129 0.0 1101 130-134 0.0 1101 135-139 0.0 1101 140-144 0.0 1101 145-149 0.0 1101 150-151 0.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 13 2.0 14 5.0 15 16.0 16 8.0 17 10.0 18 14.0 19 22.0 20 5.0 21 9.0 22 14.0 23 32.0 24 28.0 25 22.0 26 28.0 27 19.0 28 20.0 29 18.0 30 26.0 31 42.0 32 66.0 33 110.0 34 261.0 35 753.0 36 2310.0 37 160.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 48.449999999999996 22.025 9.950000000000001 19.575 2 32.375 26.8 23.625 17.2 3 25.15 27.675 28.375 18.8 4 26.950000000000003 34.675 21.075 17.299999999999997 5 25.974999999999998 36.125 20.65 17.25 6 23.45 37.3 21.224999999999998 18.025 7 23.825 22.825 33.900000000000006 19.45 8 25.124999999999996 26.150000000000002 23.525 25.2 9 26.325 25.1 26.25 22.325 10-14 27.04 28.04 23.905 21.015 15-19 26.26 27.584999999999997 25.365 20.79 20-24 26.755000000000003 27.189999999999998 25.685000000000002 20.369999999999997 25-29 26.700000000000003 28.555000000000003 24.92 19.825 30-34 25.955000000000002 27.355 25.85 20.84 35-39 25.66 28.185 25.77 20.385 40-44 26.66 27.134999999999998 25.915 20.29 45-49 26.41 27.345000000000002 26.32 19.925 50-54 24.169999999999998 28.03 26.96 20.84 55-59 25.185000000000002 27.295 26.855 20.665 60-64 25.935000000000002 28.48 25.66 19.925 65-69 25.945 27.005000000000003 26.915 20.135 70-74 24.060000000000002 28.28 26.87 20.79 75-79 24.25 28.715000000000003 26.619999999999997 20.415 80-84 25.619999999999997 28.235 26.075 20.07 85-89 26.185000000000002 27.860000000000003 25.924999999999997 20.03 90-94 25.724999999999998 28.08 26.174999999999997 20.02 95-99 25.825 28.375 26.275 19.525000000000002 100-104 25.580000000000002 28.715000000000003 25.5 20.205000000000002 105-109 25.45 28.095 26.46 19.994999999999997 110-114 26.345000000000002 27.655 25.715 20.285 115-119 26.279999999999998 28.199999999999996 26.05 19.470000000000002 120-124 25.82 27.900000000000002 26.3 19.98 125-129 26.55 28.384999999999998 25.814999999999998 19.25 130-134 26.479999999999997 27.965 26.135 19.42 135-139 26.295 28.395 25.75 19.56 140-144 26.479999999999997 28.415000000000003 25.900000000000002 19.205 145-149 27.3 28.475 24.709999999999997 19.515 150-151 26.437500000000004 28.212500000000002 25.775 19.575 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.5 5 1.0 6 1.5 7 1.5 8 0.5 9 1.0 10 1.0 11 1.5 12 2.5 13 2.5 14 2.5 15 3.0 16 3.5 17 2.0 18 1.0 19 0.5 20 0.5 21 0.5 22 1.0 23 1.0 24 0.5 25 1.5 26 1.5 27 3.0 28 4.5 29 7.0 30 12.5 31 15.0 32 20.0 33 27.0 34 32.5 35 46.0 36 52.5 37 73.0 38 105.5 39 135.5 40 173.5 41 209.5 42 218.0 43 227.5 44 272.0 45 314.0 46 292.5 47 239.5 48 213.0 49 192.0 50 168.0 51 157.5 52 137.5 53 98.5 54 90.0 55 80.5 56 46.0 57 29.0 58 32.5 59 30.5 60 25.5 61 19.0 62 11.0 63 10.5 64 10.5 65 6.5 66 4.0 67 1.5 68 1.0 69 1.0 70 1.5 71 1.5 72 1.5 73 2.0 74 1.5 75 2.5 76 4.0 77 3.5 78 2.0 79 2.5 80 3.0 81 2.0 82 2.0 83 4.0 84 4.5 85 3.5 86 9.0 87 9.5 88 6.5 89 7.5 90 5.0 91 3.0 92 3.0 93 1.5 94 2.0 95 3.0 96 1.5 97 2.0 98 3.0 99 2.5 100 11.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150-151 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 62.025 #Duplication Level Percentage of deduplicated Percentage of total 1 65.69931479242241 40.75 2 20.19347037484885 25.05 3 8.424022571543732 15.675 4 3.2245062474808543 8.0 5 1.2494961708988312 3.875 6 0.5239822652156388 1.95 7 0.2015316404675534 0.8750000000000001 8 0.16122531237404272 0.8 9 0.08061265618702136 0.44999999999999996 >10 0.24183796856106407 2.5749999999999997 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG 46 1.15 No Hit GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGT 16 0.4 No Hit GATGGGCAGCAGGTGGACAAGCTTGTGGGAGCCAACAAGCCAGAGTTACA 11 0.27499999999999997 No Hit CTCACACCGATATGGAGAAGAAGCAGGGAAGGCAACAAATGAAGGGTTTG 10 0.25 No Hit GGAGTTCTGAAATCTGTGTCAAGCACCTTACTTGGAGTGAGTCCAGAGTT 10 0.25 No Hit GTTCCTTCATGGGCTTCGCAGTTTGATACTTGGCTTAAAAATGCTCTTCT 10 0.25 No Hit AGATCAACCCTTACCTACTTGGAACTATGGCTGGTGGTGCAGCGGACTGT 9 0.22499999999999998 No Hit GGTGCCACCGATCCAACGTTTTTGTACTTTGGTCATGCTTTGAAGGAGGT 9 0.22499999999999998 No Hit ACGACAACGACAACAACAAGAGCAAAAACACCCTTATCGGAGATTGTAGA 8 0.2 No Hit GAGAAACCAGAACCTCATCAGGGGAAACAGTTTCAGTACTTTGTGCTATT 8 0.2 No Hit GAAGAGTAAAAGGCCTCAGGCTGGACCTAATTCTGGTTTCATCTCACAGC 8 0.2 No Hit TAGAACTGAAAGAGCAGAGAAGCAGTCTGCACTGCTTTTGGAGGCCTTCA 8 0.2 No Hit CCTCAATAGTGAAACCATCCATGACGTTTGGCTCAGTAGGATCCAGTGTC 7 0.17500000000000002 No Hit GTTGTAGTGCCGCTCGCAGTGTAGGATGTTATGATAGGGAAGAACTTGAA 7 0.17500000000000002 No Hit TGAAAATTCTATGGAAGAGCTAGCATCTCTGACGAAAACAGCAGACGGAA 7 0.17500000000000002 No Hit GGAATTTAGGAGGAGATTCAGAGGATACTGATATTGTTTCGCTAAAGAAT 7 0.17500000000000002 No Hit GGGAAAAGGTAGAGCAAGAGGAAGAAGACCAGCGATTAAACAACGAGAAA 7 0.17500000000000002 No Hit GCTAGATAACCCCCCTCCCTTCTCTGCTCTCCTCTCCTCCCACAGCATCC 6 0.15 No Hit GTCCTGGATCGCAGTAAAAATGCTTTCTGGATTTCATTTGATGGCATCCC 6 0.15 No Hit GGTTGCAAAAAACTCAACCTTTGTTAGAGATGTTGCGGCCAAAAGAAGTT 6 0.15 No Hit CCGTGGCTCAGGCTCGACTCAAAGCAACCGTGCCCTCTTGATGCACTGTG 6 0.15 No Hit ATCTAATACAAACACAACCCCTTTCCTCACTCGGTTTGTAGAAACTCTCA 6 0.15 No Hit ATCACTCTGGAGGTCGAGAGCTCAGACACCATTGACAACGTGAAGGCCAA 6 0.15 No Hit TAAAGAGGCATCTATCACATAAGGCATCATTATAACTAAAAATGGGATAT 6 0.15 No Hit GTCGAAGACTGTGGCCATCAGATGTGTGCACAATGCACACTGGCCCTCTG 6 0.15 No Hit CTTCGCAGCAGGTCGCAGCTTTCGCTTGAATCAAAAGAGCTCTCTTTTAG 6 0.15 No Hit AGTAAACAGAATCGCCCCAAAATTTCAAGACGGTGTAAAAGCCTTCTCTG 6 0.15 No Hit CACCTCCTGAGTATCTGAGGACAGGAAGGGTAACAGCAGAAAGTGTAATT 6 0.15 No Hit ATTGATGTTATTTTATGCAACTGTTTCCTGTAAATTTTCTGGTGAGTTTG 6 0.15 No Hit GAGACCAGCCCTGAATGAGGCTATGGAAGGAGAAAAGAATGGTCATCATC 6 0.15 No Hit GACGATCCTCAAGATGCTGTTGTAGCACAACAGTATCTTAGAGACCATCA 5 0.125 No Hit GAGAACTCAGTTAAGTAAGGAGACACGATGGCTAAGTTTGCTGTGGCTAA 5 0.125 No Hit GCCGGCTGGACACGGTGTGCGATCAAGGACCAGAGATCTCTTCGCTCGTC 5 0.125 No Hit GACAAGACCATTGCATTCTGGTTGATGGACAAGGATATGTACGACTTCAT 5 0.125 No Hit GTTCTGTCCCCGGCTAGACTAGGAGCTGATGTGGTTGTGCATAGCATCTC 5 0.125 No Hit GTCACAAACCTCTGGTATCGGATTCGTATGGAAGATCGCATCACGGACCT 5 0.125 No Hit GGTTCAGGGGAGGCCCACGCACTGTAGTCGTAGGTAAACAAGTCCTTGAA 5 0.125 No Hit GATTCATCTCTGGGGTACCTGTTTGGAGATAAGTGATGTTTCTTTCTCCA 5 0.125 No Hit GCTACTAGAAACTTCTACAGCTCTTCCTCTCTGCCTCAAATCAATCAATC 5 0.125 No Hit AGAAAACATAAAAAGAAACAATCAACAGTACTCTCAGAGCCGAAAGATGT 5 0.125 No Hit ATTTTGCTTCTTACAGTTTTCTTTGTATCCCATTTGCTTTATTTTCCTTT 5 0.125 No Hit ATCTTCGAGAAAACTCCCTGACTTCAAGCAGTCAGTGAAGCTGAAATATG 5 0.125 No Hit GCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATAGGAGGTTTCC 5 0.125 No Hit AAAGACCTCACTGCGAAAATTTCAGATTTTGGTCTGGCTAAGCTTATACC 5 0.125 No Hit AGTATCAAGAAAGCTCTATCTAATGGAGAGATGGTTAACATGAATCTTGA 5 0.125 No Hit CAAGAGGATACACATGGCATTAGACTCAGATGATGTTGAACTTGTGAAAC 5 0.125 No Hit CAATAAAATACACCATCATCCAAATCTCCTCATCAGCAATCATTCTCATA 5 0.125 No Hit CAGATGTCCCTCTCCATGCCGATCAGATCTATTTTGTGCTTCCTAAATCA 5 0.125 No Hit GTGCCCCTCGTGGTGGTTTCCGCGGTGGTCGCCGTGGTGGTTATAGCAAT 5 0.125 No Hit GGGTGAGGATGATTTATCAAGGCGGCTTGCAGAGCTTAAGGCCAAAGGGT 5 0.125 No Hit GGAATGTCTTCTAAAGGGTTTTTGTCAACTTGTACAAGCATCTGTACTGA 5 0.125 No Hit GAGAGAGCTCTCTTCCAATACTACTAGGGCTACTACTACAGTACTACTAG 5 0.125 No Hit CACAACTGGTGGGGTGTGGTACTCATATGCCAAATACCAGCAGAAGATGA 5 0.125 No Hit TCCTGATGAACGGGTTGAGTACGAGTTCTGGACAAATAGCAATGATGAGT 5 0.125 No Hit GGGTTCATCACCCTGGAAGTGAAGTGCCACAAACATGGGAGCCCAGAAAA 5 0.125 No Hit ATCACCTTAATCGATTACGAGTATGCAAGTTTCAATCCTGTTGCGTATGA 5 0.125 No Hit GATATTACAGATAAACAGAAGAAAGCTATTTATGATCTTAGCATGGGCGT 5 0.125 No Hit CCTCTTCGCTCCATTCACTTCCCAACCACTCAATTGCCTATAGCCCATCA 5 0.125 No Hit AGAAAGAATGCGGAGGATGCACTGCAGAGTTTGAATGGAACAACAATTGG 5 0.125 No Hit TCTACTTGTCACAAACCCCAAGCGTGTGGAGAAAGCAATTAAGGAGAAGT 5 0.125 No Hit GTGCTACTTACTTAACTTCCACTCTCAAACATGAGTTCCACCATGAGCAC 5 0.125 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.025 0.0 0.0 0.0 0.0 52-53 0.025 0.0 0.0 0.0 0.0 54-55 0.025 0.0 0.0 0.0 0.0 56-57 0.025 0.0 0.0 0.0 0.0 58-59 0.025 0.0 0.0 0.0 0.0 60-61 0.025 0.0 0.0 0.0 0.0 62-63 0.05 0.0 0.0 0.0 0.0 64-65 0.125 0.0 0.0 0.0 0.0 66-67 0.125 0.0 0.0 0.0 0.0 68-69 0.1375 0.0 0.0 0.0 0.0 70-71 0.16249999999999998 0.0 0.0 0.0 0.0 72-73 0.175 0.0 0.0 0.0 0.0 74-75 0.275 0.0 0.0 0.0 0.0 76-77 0.275 0.0 0.0 0.0 0.0 78-79 0.3125 0.0 0.0 0.0 0.0 80-81 0.3625 0.0 0.0 0.0 0.0 82-83 0.375 0.0 0.0 0.0 0.0 84-85 0.3875 0.0 0.0 0.0 0.0 86-87 0.475 0.0 0.0 0.0 0.0 88-89 0.55 0.0 0.0 0.0 0.0 90-91 0.5874999999999999 0.0 0.0 0.0 0.0 92-93 0.65 0.0 0.0 0.0 0.0 94-95 0.7375 0.0 0.0 0.0 0.0 96-97 0.8999999999999999 0.0 0.0 0.0 0.0 98-99 1.0750000000000002 0.0 0.0 0.0 0.0 100-101 1.2125 0.0 0.0 0.0 0.0 102-103 1.3624999999999998 0.0 0.0 0.0 0.0 104-105 1.65 0.0 0.0 0.0 0.0 106-107 1.9625 0.0 0.0 0.0 0.0 108-109 2.0625 0.0 0.0 0.0 0.0 110-111 2.225 0.0 0.0 0.0 0.0 112-113 2.4 0.0 0.0 0.0 0.0 114-115 3.0875 0.0 0.0 0.0 0.0 116-117 3.425 0.0 0.0 0.0 0.0 118-119 4.025 0.0 0.0 0.0 0.0 120-121 4.487500000000001 0.0 0.0 0.0 0.0 122-123 4.975 0.0 0.0 0.0 0.0 124-125 5.4625 0.0 0.0 0.0 0.0 126-127 6.074999999999999 0.0 0.0 0.0 0.0 128-129 6.7125 0.0 0.0 0.0 0.0 130-131 7.2125 0.0 0.0 0.0 0.0 132-133 7.699999999999999 0.0 0.0 0.0 0.0 134-135 8.075 0.0 0.0 0.0 0.0 136-137 8.925 0.0 0.0 0.0 0.0 138-139 9.6375 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TGCTTTC 10 0.006830828 145.0 3 >>END_MODULE Read 3150976 spots for SRR26075358.sra Written 3150976 spots for SRR26075358.sra Read 3150976 spots for SRR26075358.sra Written 3150976 spots for SRR26075358.sra Read 3150976 spots for SRR26075358.sra Written 3150976 spots for SRR26075358.sra Read 3150976 spots for SRR26075358.sra Written 3150976 spots for SRR26075358.sra Read 3150976 spots for SRR26075358.sra Written 3150976 spots for SRR26075358.sra Read 3150976 spots for SRR26075358.sra Written 3150976 spots for SRR26075358.sra Read 3150976 spots for SRR26075358.sra Written 3150976 spots for SRR26075358.sra Read 3150985 spots for SRR26075358.sra Written 3150985 spots for SRR26075358.sra Read 3150976 spots for SRR26075358.sra Written 3150976 spots for SRR26075358.sra Read 3150976 spots for SRR26075358.sra Written 3150976 spots for SRR26075358.sra Read 3150976 spots for SRR26075358.sra Written 3150976 spots for SRR26075358.sra Read 3150976 spots for SRR26075358.sra Written 3150976 spots for SRR26075358.sra Read 3150976 spots for SRR26075358.sra Written 3150976 spots for SRR26075358.sra Read 3150976 spots for SRR26075358.sra Written 3150976 spots for SRR26075358.sra Read 3150976 spots for SRR26075358.sra Written 3150976 spots for SRR26075358.sra Read 3150976 spots for SRR26075358.sra Written 3150976 spots for SRR26075358.sra Read 3150976 spots for SRR26075358.sra Written 3150976 spots for SRR26075358.sra Read 3150976 spots for SRR26075358.sra Written 3150976 spots for SRR26075358.sra Read 3150976 spots for SRR26075358.sra Written 3150976 spots for SRR26075358.sra Read 3150976 spots for SRR26075358.sra Written 3150976 spots for SRR26075358.sra SRR ids: ['SRR26075358.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_lisri7r3 SRR26075358.sra spots: 63019529 blocks: [[1, 3150976], [3150977, 6301952], [6301953, 9452928], [9452929, 12603904], [12603905, 15754880], [15754881, 18905856], [18905857, 22056832], [22056833, 25207808], [25207809, 28358784], [28358785, 31509760], [31509761, 34660736], [34660737, 37811712], [37811713, 40962688], [40962689, 44113664], [44113665, 47264640], [47264641, 50415616], [50415617, 53566592], [53566593, 56717568], [56717569, 59868544], [59868545, 63019529]] SRR26075358 file size 23280848 SRR26075358 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o SRR26075358 SRR26075358_1.fastq SRR26075358_2.fastq Input file: SRR26075358_1.fastq Paired file: SRR26075358_2.fastq trimmed: SRR26075358-trimmed-pair1.fastq, SRR26075358-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Wed Feb 12 03:32:39 2025 >> started Wed Feb 12 03:33:47 2025 >> done (67.668s) 63019529 read pairs processed; of these: 362 ( 0.00%) short read pairs filtered out after trimming by size control 829735 ( 1.32%) empty read pairs filtered out after trimming by size control 62189432 (98.68%) read pairs available; of these: 9689564 (15.58%) trimmed read pairs available after processing 52499868 (84.42%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 18 33 0.00% 19 28 0.00% 20 41 0.00% 21 44 0.00% 22 63 0.00% 23 85 0.00% 24 105 0.00% 25 84 0.00% 26 124 0.00% 27 150 0.00% 28 108 0.00% 29 117 0.00% 30 124 0.00% 31 149 0.00% 32 115 0.00% 33 141 0.00% 34 128 0.00% 35 144 0.00% 36 168 0.00% 37 165 0.00% 38 179 0.00% 39 181 0.00% 40 182 0.00% 41 213 0.00% 42 253 0.00% 43 218 0.00% 44 228 0.00% 45 249 0.00% 46 290 0.00% 47 301 0.00% 48 318 0.00% 49 334 0.00% 50 350 0.00% 51 469 0.00% 52 483 0.00% 53 575 0.00% 54 572 0.00% 55 587 0.00% 56 605 0.00% 57 700 0.00% 58 792 0.00% 59 1026 0.00% 60 1017 0.00% 61 1182 0.00% 62 1324 0.00% 63 1559 0.00% 64 1887 0.00% 65 1914 0.00% 66 2275 0.00% 67 2503 0.00% 68 2602 0.00% 69 3247 0.01% 70 3853 0.01% 71 4071 0.01% 72 4740 0.01% 73 5671 0.01% 74 6344 0.01% 75 7197 0.01% 76 8074 0.01% 77 8582 0.01% 78 9797 0.02% 79 11118 0.02% 80 12363 0.02% 81 14019 0.02% 82 15941 0.03% 83 18135 0.03% 84 20749 0.03% 85 22474 0.04% 86 24192 0.04% 87 26123 0.04% 88 28148 0.05% 89 29771 0.05% 90 33224 0.05% 91 36193 0.06% 92 39366 0.06% 93 43912 0.07% 94 47775 0.08% 95 49880 0.08% 96 53594 0.09% 97 57394 0.09% 98 59748 0.10% 99 64324 0.10% 100 66330 0.11% 101 69976 0.11% 102 74489 0.12% 103 79774 0.13% 104 83352 0.13% 105 88412 0.14% 106 93447 0.15% 107 95557 0.15% 108 99537 0.16% 109 103889 0.17% 110 106686 0.17% 111 112419 0.18% 112 115377 0.19% 113 120673 0.19% 114 126586 0.20% 115 132792 0.21% 116 136191 0.22% 117 140647 0.23% 118 144930 0.23% 119 146228 0.24% 120 150111 0.24% 121 156187 0.25% 122 161908 0.26% 123 164668 0.26% 124 171376 0.28% 125 174544 0.28% 126 182661 0.29% 127 186940 0.30% 128 189391 0.30% 129 193824 0.31% 130 197373 0.32% 131 199152 0.32% 132 204512 0.33% 133 211119 0.34% 134 216719 0.35% 135 222848 0.36% 136 225899 0.36% 137 227242 0.37% 138 231990 0.37% 139 236530 0.38% 140 237728 0.38% 141 242062 0.39% 142 250536 0.40% 143 249042 0.40% 144 260625 0.42% 145 259798 0.42% 146 264729 0.43% 147 268910 0.43% 148 271036 0.44% 149 269482 0.43% 150 275827 0.44% 151 52499868 84.42% 62189432 reads passed initial QC criterion=sequence-density sequence-density=0.36 sequence-density-rank=1 fanout-score=2.90 fanout-score-rank=28 prefix-density=0.49 prefix-fanout=2.2 sequence=CACTTGCAGCCATTCTC criterion=fanout-score sequence-density=0.02 sequence-density-rank=32 fanout-score=100.26 fanout-score-rank=1 prefix-density=0.17 prefix-fanout=12.8 sequence=TTCACCATTTTACTTCACCAATTCCTTAGAGATGTAATAGCCCATAACAATAGGAAATATCAGAAATCCAATAAGAATCAGCAATTCAGGAAGAAATATGACAAGGAGTAGTAGTGTGGATGTTGTTGTTAGACACTTCTTTTTGTCTTTAAATATAAGGCGTGGTAGAATTACTGGCACTCCAATGATTCCATATAACGGCCATAATGGAGCTATAGAATACAACACCAACGTCGCAAAAAACCAGCAAAAATTCTTAACATTATTTTTAGAAATCCCATACTGCCACCGAATATTCAGTCCTTTAAGAAATCGAACAGCATACCCAACATAGTAAAAACCATCAATAATGCAAATACCGTTACCACAAGTGCAAATACTCCCATTCCTACCTC criterion=sequence-density sequence-density=0.40 sequence-density-rank=1 fanout-score=2.32 fanout-score-rank=26 prefix-density=0.40 prefix-fanout=2.3 sequence=ATGTACCCTGAC criterion=fanout-score sequence-density=0.11 sequence-density-rank=28 fanout-score=31.96 fanout-score-rank=1 prefix-density=0.34 prefix-fanout=10.9 sequence=TGAGAAGAAGGAT SRR26075358 testing PE reads STAR mapping to Ensembl genome Started job on | Feb 12 03:34:33 Started mapping on | Feb 12 03:34:34 Finished on | Feb 12 03:48:52 Mapping speed, Million of reads per hour | 260.93 Number of input reads | 62189432 Average input read length | 294 UNIQUE READS: Uniquely mapped reads number | 52787433 Uniquely mapped reads % | 84.88% Average mapped length | 292.70 Number of splices: Total | 41971593 Number of splices: Annotated (sjdb) | 40999720 Number of splices: GT/AG | 41235706 Number of splices: GC/AG | 549213 Number of splices: AT/AC | 47718 Number of splices: Non-canonical | 138956 Mismatch rate per base, % | 0.44% Deletion rate per base | 0.03% Deletion average length | 2.98 Insertion rate per base | 0.02% Insertion average length | 2.65 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 1566139 % of reads mapped to multiple loci | 2.52% Number of reads mapped to too many loci | 214005 % of reads mapped to too many loci | 0.34% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 11.61% % of reads unmapped: other | 0.65% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 7835860 7835860 7835860 N_multimapping 1566139 1566139 1566139 N_noFeature 1309849 52127175 1686819 N_ambiguous 620430 3250 335878 UnstrandedReadsAssigned:50857154 PositiveStrandReadsAssigned:657008 NegativeStrandReadsAssigned:50764736 Dataset is classified negative stranded MeadianReadLen=151 20thPercentileLength=151 echo kmer=147 SRR26075358 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,400 [index] number of k-mers: 62,057,036 [index] number of equivalence classes: 130,681 [quant] running in paired-end mode [quant] will process pair 1: SRR26075358-trimmed-pair1.fastq SRR26075358-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 62,189,432 reads, 52,057,494 reads pseudoaligned [quant] estimated average fragment length: 211.46 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,286 rounds 52401 SRR26075358.ke.tsv 34699 SRR26075358.se.tsv 87100 total ==> SRR26075358.ke.tsv <== target_id length eff_length est_counts tpm Potri.005G200100.1.v4.1 2018 1807.54 8357 67.4749 Potri.005G024800.1.v4.1 1035 824.54 8125.14 143.813 Potri.004G059700.1.v4.1 961 750.54 26 0.505568 Potri.007G009000.2.v4.1 1416 1205.54 0 0 Potri.003G141000.2.v4.1 2943 2732.54 2018 10.7779 Potri.016G087400.1.v4.1 270 89.4851 5806.42 946.973 Potri.015G069301.1.v4.1 564 355 0 0 Potri.010G195200.1.v4.1 1773 1562.54 262 2.44709 Potri.012G127500.1.v4.1 977 766.54 9082 172.913 ==> SRR26075358.se.tsv <== Potri.001G166300.v4.1 0 Potri.001G448400.v4.1 317 Potri.001G233950.v4.1 2 Potri.001G122700.v4.1 582 Potri.001G212900.v4.1 0 Potri.001G182400.v4.1 0 Potri.001G256600.v4.1 0 Potri.001G040500.v4.1 27 Potri.001G416900.v4.1 2 Potri.001G452600.v4.1 2034 SRR26075358 completed mapping pipeline successfully