Starting /dee2/code/volunteer_pipeline.sh SRR26075359 current disk space = 3048982659072 free memory = 1579093780 SRR26075359 SRAfilesize c1b920b5082d5717917c8261295d09c7 SRR26075359.sra SRR26075359.sra file validated SRR26075359 is paired end SRR26075359 is conventional basespace SRR26075359 read1 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR26075359_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.57225 37.0 37.0 37.0 37.0 37.0 2 36.602 37.0 37.0 37.0 37.0 37.0 3 36.67 37.0 37.0 37.0 37.0 37.0 4 36.6445 37.0 37.0 37.0 37.0 37.0 5 36.7205 37.0 37.0 37.0 37.0 37.0 6 36.6205 37.0 37.0 37.0 37.0 37.0 7 36.6625 37.0 37.0 37.0 37.0 37.0 8 36.672 37.0 37.0 37.0 37.0 37.0 9 36.7485 37.0 37.0 37.0 37.0 37.0 10-14 36.660799999999995 37.0 37.0 37.0 37.0 37.0 15-19 36.6503 37.0 37.0 37.0 37.0 37.0 20-24 36.5663 37.0 37.0 37.0 37.0 37.0 25-29 36.4884 37.0 37.0 37.0 37.0 37.0 30-34 36.469800000000006 37.0 37.0 37.0 37.0 37.0 35-39 36.4156 37.0 37.0 37.0 37.0 37.0 40-44 36.35360000000001 37.0 37.0 37.0 37.0 37.0 45-49 36.300599999999996 37.0 37.0 37.0 37.0 37.0 50-54 36.3347 37.0 37.0 37.0 37.0 37.0 55-59 36.1409 37.0 37.0 37.0 37.0 37.0 60-64 36.095 37.0 37.0 37.0 37.0 37.0 65-69 35.989200000000004 37.0 37.0 37.0 37.0 37.0 70-74 36.0596 37.0 37.0 37.0 37.0 37.0 75-79 36.0578 37.0 37.0 37.0 37.0 37.0 80-84 36.0629 37.0 37.0 37.0 37.0 37.0 85-89 35.904399999999995 37.0 37.0 37.0 37.0 37.0 90-94 35.8903 37.0 37.0 37.0 37.0 37.0 95-99 35.82190000000001 37.0 37.0 37.0 37.0 37.0 100-104 35.895799999999994 37.0 37.0 37.0 37.0 37.0 105-109 35.7073 37.0 37.0 37.0 37.0 37.0 110-114 35.6841 37.0 37.0 37.0 37.0 37.0 115-119 35.55820000000001 37.0 37.0 37.0 37.0 37.0 120-124 35.643 37.0 37.0 37.0 37.0 37.0 125-129 35.4379 37.0 37.0 37.0 37.0 37.0 130-134 35.3861 37.0 37.0 37.0 37.0 37.0 135-139 35.2057 37.0 37.0 37.0 29.8 37.0 140-144 35.058499999999995 37.0 37.0 37.0 25.0 37.0 145-149 34.9855 37.0 37.0 37.0 25.0 37.0 150-151 34.708 37.0 37.0 37.0 25.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.0 1101 2 0.0 1101 3 0.0 1101 4 0.0 1101 5 0.0 1101 6 0.0 1101 7 0.0 1101 8 0.0 1101 9 0.0 1101 10-14 0.0 1101 15-19 0.0 1101 20-24 0.0 1101 25-29 0.0 1101 30-34 0.0 1101 35-39 0.0 1101 40-44 0.0 1101 45-49 0.0 1101 50-54 0.0 1101 55-59 0.0 1101 60-64 0.0 1101 65-69 0.0 1101 70-74 0.0 1101 75-79 0.0 1101 80-84 0.0 1101 85-89 0.0 1101 90-94 0.0 1101 95-99 0.0 1101 100-104 0.0 1101 105-109 0.0 1101 110-114 0.0 1101 115-119 0.0 1101 120-124 0.0 1101 125-129 0.0 1101 130-134 0.0 1101 135-139 0.0 1101 140-144 0.0 1101 145-149 0.0 1101 150-151 0.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 20 1.0 21 0.0 22 1.0 23 6.0 24 4.0 25 2.0 26 13.0 27 11.0 28 14.0 29 28.0 30 34.0 31 43.0 32 50.0 33 115.0 34 202.0 35 462.0 36 2822.0 37 192.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 36.88298672012027 14.683036832873967 9.145577549486344 39.288398897519414 2 17.974999999999998 15.049999999999999 34.25 32.725 3 17.525 14.674999999999999 30.7 37.1 4 23.599999999999998 24.325 21.2 30.875000000000004 5 22.925 28.675 25.874999999999996 22.525000000000002 6 23.75 31.25 23.05 21.95 7 17.65 30.55 36.575 15.225 8 17.4 30.225 29.425 22.95 9 17.299999999999997 25.025 35.025 22.650000000000002 10-14 19.465 30.075000000000003 26.86 23.599999999999998 15-19 19.59 28.249999999999996 27.41 24.75 20-24 20.455000000000002 27.365000000000002 28.705000000000002 23.474999999999998 25-29 20.385 28.26 27.134999999999998 24.22 30-34 20.09 27.860000000000003 28.15 23.9 35-39 21.115000000000002 27.37 27.169999999999998 24.345 40-44 18.96 28.435 28.544999999999998 24.060000000000002 45-49 19.49 27.24 28.585 24.685000000000002 50-54 19.97 26.845000000000002 28.09 25.095 55-59 20.349999999999998 26.979999999999997 27.925 24.745 60-64 21.224999999999998 27.075 27.76 23.94 65-69 20.885 27.165 27.16 24.79 70-74 20.465 27.1 28.515 23.919999999999998 75-79 21.455 27.79 26.384999999999998 24.37 80-84 20.75 27.905 26.91 24.435000000000002 85-89 21.19 27.47 27.36 23.98 90-94 22.165000000000003 27.025 26.950000000000003 23.86 95-99 21.2 27.200000000000003 27.845 23.755000000000003 100-104 21.375 27.450000000000003 27.055 24.12 105-109 21.84 28.455000000000002 26.284999999999997 23.419999999999998 110-114 21.98 27.01 26.855 24.154999999999998 115-119 21.305 27.800000000000004 26.55 24.345 120-124 22.425 27.884999999999998 26.395000000000003 23.294999999999998 125-129 21.6 26.145000000000003 27.43 24.825 130-134 22.835 27.765 25.885 23.515 135-139 22.52 27.525 26.495 23.46 140-144 23.1 27.505000000000003 25.56 23.835 145-149 21.790000000000003 27.74 25.595000000000002 24.875 150-151 22.8125 26.387500000000003 25.9875 24.8125 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 0.0 1 0.5 2 0.5 3 0.0 4 0.5 5 1.0 6 0.5 7 0.0 8 0.0 9 0.0 10 0.0 11 0.5 12 0.5 13 0.0 14 0.0 15 1.0 16 1.0 17 0.0 18 4.0 19 4.5 20 0.5 21 0.0 22 1.5 23 2.0 24 1.0 25 1.5 26 5.5 27 6.0 28 3.0 29 6.5 30 9.5 31 25.0 32 38.0 33 48.5 34 49.5 35 52.0 36 80.5 37 102.5 38 118.0 39 140.5 40 167.0 41 182.5 42 211.0 43 250.5 44 236.0 45 237.5 46 259.5 47 226.5 48 235.5 49 226.5 50 179.5 51 173.0 52 140.0 53 101.0 54 89.0 55 85.0 56 59.0 57 42.0 58 35.5 59 17.5 60 17.0 61 19.0 62 24.0 63 19.5 64 8.0 65 4.5 66 9.5 67 14.5 68 8.5 69 4.0 70 5.0 71 3.0 72 1.0 73 0.5 74 0.5 75 0.5 76 0.0 77 0.5 78 0.5 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.22499999999999998 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150-151 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 57.199999999999996 #Duplication Level Percentage of deduplicated Percentage of total 1 61.319930069930074 35.075 2 21.503496503496503 24.6 3 8.916083916083917 15.299999999999999 4 3.802447552447553 8.7 5 1.8793706293706292 5.375 6 1.0926573426573427 3.75 7 0.8304195804195804 3.325 8 0.26223776223776224 1.2 9 0.17482517482517482 0.8999999999999999 >10 0.21853146853146854 1.775 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAAGGTCTATCTCGTAT 27 0.675 TruSeq Adapter, Index 2 (97% over 36bp) CCTGAAAGAAGTTCTGCCAACACAAGAAATTAAGACGGGCATTTTATTTA 11 0.27499999999999997 No Hit CCTGGATCATGGAGCCAGTGATTTTCTCAACACTGTATGCAGTTTTGTAC 11 0.27499999999999997 No Hit CACTCAACCACCTGCAGCACAAATGAGGAGTGCTTGCAATCACCTTCCTT 11 0.27499999999999997 No Hit GTTGTCACAAAGCTAAAATGAGGCCACGCATTTAAGTGGAATCATTTCAG 11 0.27499999999999997 No Hit GTTGGTTTCATTCATAGCAAGATTGGCAATTGCACCTGCTGCAACTCTAT 9 0.22499999999999998 No Hit CCTGACAACCAGGGAGGTCCTGGGGGACGGTTGGGTGTTCTTCAAGGACA 9 0.22499999999999998 No Hit CTTAGCCATATATCATAATCTATAGGATATGGTGTCCCACATAGACTGTC 9 0.22499999999999998 No Hit ATCAGCAAGAGAAGCGACAAGAAGCTTAGCGTTTGGGAGAGAGCGAGCCA 9 0.22499999999999998 No Hit GGCCCAACATGTGCAGGGATTGGAATCGGGTATGCACCATTCTGCAAAAT 8 0.2 No Hit CTCCTTAGCAATGGCATAGATGTATGGCAAATTAACCATGTCTTTCTCTT 8 0.2 No Hit CTAATATTTACATAATTGGTTTTTTATTACATTGACGGTAGATTCACCTG 8 0.2 No Hit GCTGTGATGGTCATGCTGAAGTTCTCCTGGCAAATCCCACACACAGCCTC 8 0.2 No Hit TTGGATATGGACAACCATAAGCATCTGTTAGCAGTTTAAGCTCCGAAAGA 8 0.2 No Hit GAGTTCTAGGATGGCAAGCATTCTACCAATACATACAGGCATCTACTTCC 8 0.2 No Hit CACATCAGCACTCACCGTCTTCGCTGCACTATTATCACCATTTGCGCTTG 7 0.17500000000000002 No Hit TCAGGCATTCCATCAAAGCGGATGGCCGCAATGATTGATAGTATTCTCAA 7 0.17500000000000002 No Hit TTTTAATTTAAACCTGTTCTTGTTATTAAAAAAAAACCAGAAGGCACATG 7 0.17500000000000002 No Hit CTTAGCACCGGATCAGGCTTTAGTGTTTCGTAAGGCAATCTTCCATGAAC 7 0.17500000000000002 No Hit CTTCACCTTCTTGTCCTCCTCGGCAAATTCCTCTGCCTCACGAACCATGC 7 0.17500000000000002 No Hit CCCTGGGCCAAGGACACAGCATTTTTCGCTCCTCGGACCAATTCCTGTAT 7 0.17500000000000002 No Hit CTCTGCCGCCCACAACTTTTGGAGTATAGTGTCTCTTCTCATCCATAGCT 7 0.17500000000000002 No Hit GCAACAAAATACAGTTTTTTTTTAAGGGTGCAAGCAGCAAATAAACAGCT 7 0.17500000000000002 No Hit GCATATCATTGTTGTTACTGGATTCTGGCAAAAGATCAGCAGGAGATGAT 7 0.17500000000000002 No Hit GGGATCCATTTAAAGCCGCAGCTTATGCGGTTTAAGGGCATTATATGTTA 7 0.17500000000000002 No Hit GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAAGGTCTATCGCGTAT 7 0.17500000000000002 TruSeq Adapter, Index 2 (97% over 36bp) CTGCATAGTTACTTTCTAATTTACTTGGGAAGCAAAGCGAGCACCTTGCC 7 0.17500000000000002 No Hit GTTGGGACATTGAAAGTTGATTAAGAGCAAAAAGGATATGAATCATTTCC 7 0.17500000000000002 No Hit CCTCGCTCTCCCCTTCTTAACCGTCGCAACCGAAACCGGTCCCACCATCC 7 0.17500000000000002 No Hit GCCATCCTTGTCCAGCGCTGTAAACAAGCTTGGAAGAACCGTGTTGTTAT 7 0.17500000000000002 No Hit GCATTACTTGGAATAGCTCGTATCATGTATGTAGGATCTATATATTTCAT 7 0.17500000000000002 No Hit CACATTTGGTGGAAAAGCAGGTGCAGTGGCAGGAGCTGGTGCTGGAGCAG 7 0.17500000000000002 No Hit CGCGCCGACACTGGGGTTGTAGGGGTCACTGGTGTCGTCGGTGTCCCTGG 7 0.17500000000000002 No Hit CGCATCGGCGACAAGAGCATAGTCTTTTATGAATTTCTCAATTTGTTCAT 7 0.17500000000000002 No Hit ATCGAGTTTAATTATTAAGAAGAAATCTAAAACATCTCTAAGACTGTTAA 6 0.15 No Hit CCCAACCAAATAAAATAATTATAAAAATTCAAGGGTCTTTTTGTCTTAGA 6 0.15 No Hit TAGTCGTAACCGCGAAGCAGCAGAAAACAAACACAAGAGAGAAAACAGCG 6 0.15 No Hit CTTGCGTCATGCCACCGCCACCGCCCGGGAGCTTGTCCTTCATCTGGTCA 6 0.15 No Hit CCCGTGACCAATCGAGGGTCCCACAATGCAGGTTAGGGCAGTGAAGAGCA 6 0.15 No Hit GGCCACTTCAAAGATCTCTGTTGCAACAGATAGACGACCCTTACGTCCAT 6 0.15 No Hit CCCCATCCAAAACCTTATTAGATAATCCCATGTGGTCTTCAAGATAGACC 6 0.15 No Hit GTCGCTATCACAACCCATTGCTCTTTTATAGCTGGGACCCATTTAGTTTT 6 0.15 No Hit CCTAGTATCCACCAAGCTCCTACTAGTGCTGGGCATCATCATAATGGCCA 6 0.15 No Hit CCAGCCGCATCGAAAACAGTAGCAGGCATTCAAAAAGAAAGTCGTCTCGG 6 0.15 No Hit CACCAAGGCACACAAGGCTTTTGCATATTATGGCATCAAATTTACATAAA 6 0.15 No Hit GCGGGAAACCACTGGCCTGCCTCAGGGGCTGGGATGAAACTGTCAACGGA 6 0.15 No Hit TCCAAGAGAGATGCAGGGAAATGTTCATCACCAATGACAAATGTGCCACT 6 0.15 No Hit CATCTATGCCGGACGGCATTGCTGGTGAAGCGGTCATGCACGAGGTGGGC 6 0.15 No Hit GCCCAACAACTCAAAAGAACAAACATTTGCTTCGTACTATCTCTCTCTCA 6 0.15 No Hit TTTTTTTTTGGATTTTTTCCGCTTTGATATTCTCTGCATCCTATTTAGGG 6 0.15 No Hit CTAGAATCATTGGAATATAAGCCGTCTTTATATTTTGTTGAACGCAACAA 6 0.15 No Hit CAGGGATAAAACTAGAACTAATAAAGCAAGCAATTGCACTTTGAAGCCAG 6 0.15 No Hit ATAAAGAGAAACCAGTCCTGAGGGACAGACAGAGAGAGAGATTCAGAGAT 6 0.15 No Hit GCATAGGTCATTCCCAAACGAAGCTTAAACGGAATGCAATCAGTAATTTA 6 0.15 No Hit CTGCCAATTCCGATTGAGTTTCCTGGATTGAACCCAATGCTCCTACTGCT 6 0.15 No Hit GAGGGTTTCTCCCATTGAATGGACCATCATCAAGTTCAAAAACCTCGATT 6 0.15 No Hit ATTCCATTAAACCACACTTTAAGGCCATAGACATGGCCCTTACATAACAT 6 0.15 No Hit GTGTGAAGTTACGTGGGTCATCTTCAGGGAGTTGTTTCATTAGAGCAATG 6 0.15 No Hit CCCTGAACATTTTCCAAAACACAAGCCTTCCTTCCAAAACTGTTGCTGTC 6 0.15 No Hit CTTTGTGAGCTTTTCAGCATCTCCAAACCACTTTGAAGTAAGAGTTGAAC 5 0.125 No Hit CAGAAATTTTATTTGAAGAAGTAAGAAGAAGCTCCTCGTCTACGTTTAGC 5 0.125 No Hit ACGAAGAATCTTGTAAAAGAGATGAGCACCACTTGTTTGTCCATGTCCTT 5 0.125 No Hit AGCCATCTTTGCCTTCTGCCCGTTGCCTCCTCCCATTTTTTCTTCTTCGA 5 0.125 No Hit TCCATAGATTGATTCTCCTCCGGTTCCATTCCCCGCAGTAAAATCCCCTC 5 0.125 No Hit GCTCACTCTCCCACCCATTAATTACAGCATCTTCTCTCTTGAGCCTATTA 5 0.125 No Hit CTCTGGTGTTGTAGTGAAATAATCAGAAGCCATGATCTTATTGAGCTTCT 5 0.125 No Hit CTCCGTGATTAAGCAACACTTTAACGCTCTCAATTCTTCCCTTAAAGGCA 5 0.125 No Hit CCCTCCAGCACAAATTTCTATAGCATTAGGGAAGATCCCAGCTGTCTTCG 5 0.125 No Hit GCTGCAACTCTGTAGTACTCGCTGATTCTTCTTTTGGTGACCTGTCACCA 5 0.125 No Hit TGTCAGTATCGCGACCGTCAGTTGCTCAAATGAATCTAACTGGAATTGCT 5 0.125 No Hit CAGAGCAGTTGTGGACCAAGCTTTTTGCTGCAATTGAATTCAGCTTCTCA 5 0.125 No Hit TATTGAATTACTTGTCTGATTTATACTAAGAAAATTAAAACACTCAGCTA 5 0.125 No Hit CTGAAGTTCCTTTGATGGTTCTCCACATGCGTACTGCAGCATAGCCTGCA 5 0.125 No Hit ATCAACAATGCCTTGGGAGCGCTGAGAGGTGAAGATGAACTCTCCCAGGT 5 0.125 No Hit ACAGTATCGACATCAAATAATGAATCCCCACTCCGGACAGATGGTATAAG 5 0.125 No Hit GCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGCTGGTCGTCTACC 5 0.125 No Hit CATCCTGTCCAATGGTCAATCCGCTCAGAAGTTCCATTTTCTTATTCCTG 5 0.125 No Hit GTACTTATGCCTTCAAGATCACTACTAGTTATGCCTGCAACTTGCAGTCC 5 0.125 No Hit GTTGGCCAAGTCAACAAGGTTCATCGTGCTCAATCCTGGCATGTCCAGCG 5 0.125 No Hit CCCTGTCCCTCCGCTTAGAAGCAGATGTGCTGCTCTTCCATTGCTCCTTC 5 0.125 No Hit CACCATTGTTGCGGATGAGAAAGTCCCTCTCTTCTGAAGAAAGAAGCGTT 5 0.125 No Hit CCTGAGACATGTTGTCTGAATCTAATTTTATACTTTTCCTTCCAGCTTCC 5 0.125 No Hit ATGCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTAT 5 0.125 No Hit CGTGCTTATAATACATAACTGACTAATTTTCATTCACATCTACATGAAAA 5 0.125 No Hit CGCTCTGAGAACCCGTCATCCTCGCCAATATCACAAACGACATCCCACCA 5 0.125 No Hit CTTCGGTCCATAATTCCTCCTCAACACCTCTGGCACTCCATACGTCAACC 5 0.125 No Hit GTCGGATCACATTTTAAGCCACTTTGGCTTGACAGGTTTTTTCCCAGCAG 5 0.125 No Hit ACCCCCTCTCTCAAAAATCTTCAAGTCTTAACAGGCATGGTACTACTGCA 5 0.125 No Hit GCTCAAGAAAAAAGTGGTAGTCTTTGCTCTCCCGAGGCATACGAAATGAT 5 0.125 No Hit GAGCTGGAGACATGTGACTCAGCTGCTGGGGGTTGAGACACTGCAGCAGT 5 0.125 No Hit CGCCTAACATGAACCTTTCAAGAATAGACTCTACAAGTCATACTGAGTAG 5 0.125 No Hit GAGGTGGTGACTTGTAAACTGGCGGTGGAGGAGGAGGGGACTTGTAAACT 5 0.125 No Hit CCTCTCCAGCAGCCTCTTCCCCTCAAATTGTTCTGAGACAATCTCAATAG 5 0.125 No Hit TGGCAGCTTCTTAGTCGTGGTTCTGGCACAACCCAGTTGTCCCTGTAGAC 5 0.125 No Hit CCCATGATCACAATCTCACCTCGTAAGCATTTCTCAAGTATCTCGTTGGC 5 0.125 No Hit CCGGCGTTGATTCTGAGCCAGGATCAAACTCTCGTTTAATCTTGGTGTGT 5 0.125 No Hit CAGCTGATGGGGGGTAAGCTAGATTTAGATCTTGACCTTCATGGATCTTA 5 0.125 No Hit CCTAAACACAAAATAAAGAAAAAAAAGGAACCATTTTGATATCACTATGT 5 0.125 No Hit GTTTGGTAACACCAATCATGGATCGACCAACTTTAACGACGTTTAATCCT 5 0.125 No Hit CCCATTTTTAGTTATAATGATGCCTTATGTGATAGATGCCTCTTTAAAAT 5 0.125 No Hit CTTGGATAGCATCTTATGGTTTGCATAAACCTTCAAAAGTGTAGAATAAT 5 0.125 No Hit TCCAGGAATTCCCCACAAGTCTCAATACTAACTTCAGATTTACAGCCTTC 5 0.125 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0125 0.0 0.0 0.0 0.0 62-63 0.037500000000000006 0.0 0.0 0.0 0.0 64-65 0.0625 0.0 0.0 0.0 0.0 66-67 0.075 0.0 0.0 0.0 0.0 68-69 0.075 0.0 0.0 0.0 0.0 70-71 0.125 0.0 0.0 0.0 0.0 72-73 0.15 0.0 0.0 0.0 0.0 74-75 0.15 0.0 0.0 0.0 0.0 76-77 0.21250000000000002 0.0 0.0 0.0 0.0 78-79 0.3375 0.0 0.0 0.0 0.0 80-81 0.5125 0.0 0.0 0.0 0.0 82-83 0.575 0.0 0.0 0.0 0.0 84-85 0.7625 0.0 0.0 0.0 0.0 86-87 0.875 0.0 0.0 0.0 0.0 88-89 1.0625 0.0 0.0 0.0 0.0 90-91 1.2374999999999998 0.0 0.0 0.0 0.0 92-93 1.35 0.0 0.0 0.0 0.0 94-95 1.4375 0.0 0.0 0.0 0.0 96-97 1.5499999999999998 0.0 0.0 0.0 0.0 98-99 1.8125 0.0 0.0 0.0 0.0 100-101 2.0 0.0 0.0 0.0 0.0 102-103 2.375 0.0 0.0 0.0 0.0 104-105 2.625 0.0 0.0 0.0 0.0 106-107 3.025 0.0 0.0 0.0 0.0 108-109 3.5875000000000004 0.0 0.0 0.0 0.0 110-111 4.0375 0.0 0.0 0.0 0.0 112-113 4.4125 0.0 0.0 0.0 0.0 114-115 4.725 0.0 0.0 0.0 0.0 116-117 5.237500000000001 0.0 0.0 0.0 0.0 118-119 5.525 0.0 0.0 0.0 0.0 120-121 5.8375 0.0 0.0 0.0 0.0 122-123 6.487500000000001 0.0 0.0 0.0 0.0 124-125 7.3125 0.0 0.0 0.0 0.0 126-127 7.8875 0.0 0.0 0.0 0.0 128-129 8.4625 0.0 0.0 0.0 0.0 130-131 9.1125 0.0 0.0 0.0 0.0 132-133 10.2625 0.0 0.0 0.0 0.0 134-135 11.149999999999999 0.0 0.0 0.0 0.0 136-137 12.274999999999999 0.0 0.0 0.0 0.0 138-139 13.3 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGTTTCT 10 0.006830828 145.0 3 TGATGAG 10 0.006830828 145.0 9 CACATTT 10 0.006830828 145.0 1 CACGTTT 10 0.006830828 145.0 1 TTTCTGA 10 0.006830828 145.0 5 TTCTGAT 10 0.006830828 145.0 6 ATATGTA 10 0.006830828 145.0 145 >>END_MODULE SRR26075359 read2 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR26075359_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.27725 37.0 37.0 37.0 37.0 37.0 2 36.32 37.0 37.0 37.0 37.0 37.0 3 36.35 37.0 37.0 37.0 37.0 37.0 4 36.4465 37.0 37.0 37.0 37.0 37.0 5 36.379 37.0 37.0 37.0 37.0 37.0 6 36.3085 37.0 37.0 37.0 37.0 37.0 7 36.2445 37.0 37.0 37.0 37.0 37.0 8 36.4755 37.0 37.0 37.0 37.0 37.0 9 36.4385 37.0 37.0 37.0 37.0 37.0 10-14 36.3811 37.0 37.0 37.0 37.0 37.0 15-19 36.29959999999999 37.0 37.0 37.0 37.0 37.0 20-24 36.251 37.0 37.0 37.0 37.0 37.0 25-29 36.1772 37.0 37.0 37.0 37.0 37.0 30-34 36.0124 37.0 37.0 37.0 37.0 37.0 35-39 35.976299999999995 37.0 37.0 37.0 37.0 37.0 40-44 35.9639 37.0 37.0 37.0 37.0 37.0 45-49 35.852500000000006 37.0 37.0 37.0 37.0 37.0 50-54 35.755900000000004 37.0 37.0 37.0 37.0 37.0 55-59 35.804700000000004 37.0 37.0 37.0 37.0 37.0 60-64 35.8617 37.0 37.0 37.0 37.0 37.0 65-69 35.757799999999996 37.0 37.0 37.0 37.0 37.0 70-74 35.6494 37.0 37.0 37.0 37.0 37.0 75-79 35.5856 37.0 37.0 37.0 37.0 37.0 80-84 35.6183 37.0 37.0 37.0 37.0 37.0 85-89 35.660399999999996 37.0 37.0 37.0 37.0 37.0 90-94 35.6323 37.0 37.0 37.0 37.0 37.0 95-99 35.717499999999994 37.0 37.0 37.0 37.0 37.0 100-104 35.541 37.0 37.0 37.0 34.6 37.0 105-109 35.6491 37.0 37.0 37.0 37.0 37.0 110-114 35.544799999999995 37.0 37.0 37.0 37.0 37.0 115-119 35.5313 37.0 37.0 37.0 37.0 37.0 120-124 35.47485 37.0 37.0 37.0 37.0 37.0 125-129 35.395 37.0 37.0 37.0 34.6 37.0 130-134 35.3585 37.0 37.0 37.0 34.6 37.0 135-139 35.2041 37.0 37.0 37.0 32.2 37.0 140-144 35.18115 37.0 37.0 37.0 32.2 37.0 145-149 35.129349999999995 37.0 37.0 37.0 27.4 37.0 150-151 34.798375 37.0 37.0 37.0 25.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.0 1101 2 0.0 1101 3 0.0 1101 4 0.0 1101 5 0.0 1101 6 0.0 1101 7 0.0 1101 8 0.0 1101 9 0.0 1101 10-14 0.0 1101 15-19 0.0 1101 20-24 0.0 1101 25-29 0.0 1101 30-34 0.0 1101 35-39 0.0 1101 40-44 0.0 1101 45-49 0.0 1101 50-54 0.0 1101 55-59 0.0 1101 60-64 0.0 1101 65-69 0.0 1101 70-74 0.0 1101 75-79 0.0 1101 80-84 0.0 1101 85-89 0.0 1101 90-94 0.0 1101 95-99 0.0 1101 100-104 0.0 1101 105-109 0.0 1101 110-114 0.0 1101 115-119 0.0 1101 120-124 0.0 1101 125-129 0.0 1101 130-134 0.0 1101 135-139 0.0 1101 140-144 0.0 1101 145-149 0.0 1101 150-151 0.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 13 3.0 14 3.0 15 4.0 16 1.0 17 3.0 18 2.0 19 3.0 20 7.0 21 2.0 22 6.0 23 10.0 24 8.0 25 19.0 26 23.0 27 16.0 28 16.0 29 18.0 30 29.0 31 32.0 32 50.0 33 72.0 34 161.0 35 635.0 36 2676.0 37 201.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 40.910227556889225 20.68017004251063 13.178294573643413 25.23130782695674 2 30.65 25.650000000000002 27.200000000000003 16.5 3 21.825 29.375 29.775000000000002 19.025 4 23.65 35.25 22.275 18.825 5 28.249999999999996 32.800000000000004 21.525 17.424999999999997 6 21.125 38.15 23.25 17.474999999999998 7 21.65 22.25 38.05 18.05 8 23.0 25.424999999999997 27.750000000000004 23.825 9 23.775 26.275 28.825 21.125 10-14 26.590000000000003 28.02 25.055 20.335 15-19 25.040000000000003 28.225 25.595000000000002 21.14 20-24 25.575 29.115000000000002 25.22 20.09 25-29 25.259999999999998 27.800000000000004 25.935000000000002 21.005 30-34 24.88 27.034999999999997 26.99 21.095 35-39 25.405 27.05 26.46 21.085 40-44 24.83 27.13 27.134999999999998 20.905 45-49 25.22 28.345 26.534999999999997 19.900000000000002 50-54 25.185000000000002 27.950000000000003 26.665 20.200000000000003 55-59 25.4 27.875 26.02 20.705000000000002 60-64 25.55 27.855 25.474999999999998 21.12 65-69 24.73 28.21 26.39 20.669999999999998 70-74 25.900000000000002 27.87 25.845000000000002 20.385 75-79 24.05 28.835 26.450000000000003 20.665 80-84 24.805 27.97 26.229999999999997 20.995 85-89 24.585 28.345 26.865 20.205000000000002 90-94 25.395 26.56 26.615 21.43 95-99 26.16 27.21 26.025 20.605 100-104 25.324999999999996 27.665 26.474999999999998 20.535 105-109 25.705 28.395 25.435000000000002 20.465 110-114 25.69 27.639999999999997 27.04 19.63 115-119 25.230000000000004 29.110000000000003 25.419999999999998 20.24 120-124 26.126306315315766 27.74138706935347 26.70133506675334 19.43097154857743 125-129 25.775 27.96 26.14 20.125 130-134 27.045 28.675 25.705 18.575 135-139 26.4026402640264 28.40784078407841 25.942594259425945 19.246924692469246 140-144 27.594139120868128 27.69415412311847 25.633845076761514 19.077861679251885 145-149 27.636909227306827 28.20205051262816 25.021255313828455 19.13978494623656 150-151 28.153519189898734 27.21590198774847 25.61570196274534 19.01487685960745 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.5 5 0.5 6 0.0 7 0.0 8 1.0 9 1.0 10 0.0 11 0.0 12 0.0 13 0.5 14 1.0 15 0.5 16 0.0 17 0.5 18 1.0 19 1.0 20 0.5 21 1.0 22 2.0 23 1.0 24 0.0 25 0.0 26 0.0 27 1.5 28 1.5 29 1.0 30 8.0 31 11.5 32 11.5 33 16.0 34 26.0 35 47.5 36 66.0 37 77.0 38 117.0 39 163.0 40 179.0 41 204.0 42 224.5 43 244.5 44 272.0 45 275.5 46 266.5 47 249.5 48 234.5 49 217.0 50 184.5 51 158.0 52 124.5 53 105.5 54 90.5 55 65.5 56 72.5 57 64.5 58 38.0 59 28.5 60 24.5 61 14.0 62 7.5 63 11.5 64 12.5 65 7.0 66 3.0 67 1.5 68 0.5 69 2.0 70 2.0 71 0.5 72 0.0 73 0.5 74 1.0 75 0.5 76 1.0 77 1.5 78 1.0 79 1.5 80 2.5 81 1.5 82 2.0 83 4.5 84 3.5 85 3.0 86 3.5 87 5.5 88 5.0 89 2.0 90 1.0 91 0.5 92 1.5 93 1.0 94 0.0 95 0.5 96 0.5 97 1.0 98 1.5 99 1.0 100 3.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.025 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.005 125-129 0.0 130-134 0.0 135-139 0.01 140-144 0.015 145-149 0.025 150-151 0.0125 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 57.775 #Duplication Level Percentage of deduplicated Percentage of total 1 62.22414539160537 35.949999999999996 2 20.813500649069667 24.05 3 8.697533535266118 15.075 4 3.937689311986153 9.1 5 1.8606663781912594 5.375 6 1.081782778018174 3.75 7 0.7788836001730853 3.15 8 0.21635655560363476 1.0 9 0.043271311120726956 0.22499999999999998 >10 0.34617048896581565 2.325 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG 17 0.42500000000000004 No Hit GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGT 15 0.375 No Hit GAATGGACAGTACTGCTCTACCAGGCCCATGCGGATTGGACCAGCTGCCA 11 0.27499999999999997 No Hit GATGAAGCTGATGGTTTCATCAAGCAGAAGCAAAAGGGCTTTGAACTGTG 10 0.25 No Hit GGAACTGAAAGCTCCGCAGTGACCGTGGAATGGGGAATTTCAGAGATCTT 10 0.25 No Hit CTTGCCTTCTAATTTCAGTCCAAGCATAATTCAGACGGTACTTAATCAAC 10 0.25 No Hit ATAATCTACATGGAAGACATAAAAGCTTGTATAGCATTCATTGCAAAATG 10 0.25 No Hit TTTGTATAGAGAGCGAGCCGCTAGCTAGCTGCTCTGCAGGGCGCTGTTCT 10 0.25 No Hit GCAGAGAGCCTGGTATATGTGCTCTACTTTGCCTGTGGTGGAGCTTTGCC 9 0.22499999999999998 No Hit GCCCTTCAAAAAGGAAGCAAGGACAACATCACTGTAATTGTGGTGGATCT 8 0.2 No Hit CTTGTCTTTGCTTGAAGCAGAAGATGTTGATGTGCGAATTCATGCTGTGA 8 0.2 No Hit CCTGAAGAGGCTGCTTTTGTTTATTGGTTTTTGTGTTTTCCTTGCTCTTA 8 0.2 No Hit GAACAGAGTGCCCGAGAATTCCACTTTTTCTTCTGTCTTGGTGTTTCTCC 8 0.2 No Hit CCCAGGCAATGAAACTCAATACTTGGCAATTTTGCAGAATGGTGCATACC 8 0.2 No Hit CCACCAACCAACCCAGCCCTCTCTGCCTCTCTCTCTCTCTGAATCACCAT 7 0.17500000000000002 No Hit TGCCAATGGTATCCTGAATGTCAAGGCCGAAGACAAGGGCACTGGTAAAT 7 0.17500000000000002 No Hit CAAACACTGTATTCCTTTTGCTTCACTTTAGGCACTATTGTTTACTTGAT 7 0.17500000000000002 No Hit CGTAGATCATGTGGAAAAGAAACTATCTCAGATGATTCTGGATAAAAAAT 7 0.17500000000000002 No Hit ATATTACACGCATAAATAGATATGGAGGAGATTCGCCGGGCTGCTGGAGC 7 0.17500000000000002 No Hit CTTGATGTTAGAATGAATTGCATTTTGGATCTTCCTGGTCAGAGATCAGC 7 0.17500000000000002 No Hit CTTGTACCGTAAATTTAGATCTTGGATCGCAGATCTATTGGGGAAATAAT 7 0.17500000000000002 No Hit GTTTTACTTTTTGCTCCCTGTTAGGAGTATTTTGGGCGGGGCGTTAGCGC 7 0.17500000000000002 No Hit TGATGTTGTTGCTGGACCTCAGCCAGAACGTGGCCTTGGCAAGCTTAGAA 7 0.17500000000000002 No Hit GCTATATATAAATATGAATATATCTTTTTTTTTCTTTCTTTTTTCGAGGT 7 0.17500000000000002 No Hit AAACATTGAAGAGTTCATGTTGCATAAGCTACATATTGAAGAAAGTGAAG 7 0.17500000000000002 No Hit GAAGGAACTGGGCAGGAATATGTTGCTCAGAACATGCATGCAGTTGATGA 7 0.17500000000000002 No Hit TTTATGATAAGAAGGAGCTTCAATTTTTGAGTCATCCTGTTAACCATGAT 7 0.17500000000000002 No Hit CGAAGTCTCACGCCGCATTGAGGAGGAGGCCTTTTCTGTTGCCACCTCCT 7 0.17500000000000002 No Hit GGAAGGACAGACAATGAGATTAAGAACCACTGGAACACTCATATAAAGAA 7 0.17500000000000002 No Hit GCAAGAGAAGCAGCAGATGCAGGGGGAGTTCTGATGCGCATGAAGGATAG 7 0.17500000000000002 No Hit GTTTTGGCAAATATTGCTTCTGTGGCGAGTGTGGCATCTCCATTAAAACC 7 0.17500000000000002 No Hit AGAAAAGGAGTGAGATATTCAAGAGAAATACGTTTAGAAATCAAGAGAGA 7 0.17500000000000002 No Hit ATAAACGAGCCTGATCCTGAAAATCCCACAAACTGTTGCCCTCCATTAAC 6 0.15 No Hit CTCGCCGATTCGACGACTAATGGATCTCTGTGACGAGCTTTTGGACCAGA 6 0.15 No Hit CAGTGAACGGCTTTTCCATGGGTTGAAAACACAATACTCTACTAGGAGAG 6 0.15 No Hit CGGGAATATAGGTTCGATCTCCAATCTGTCCATTATGTGCGGCCGATAGC 6 0.15 No Hit GACACAACGATGGGCACATCTTCTGCGATAACTATACTTTACTTCGTCGA 6 0.15 No Hit GAAAAATTTGGGGTTAAAGGGATTTTGGGTTGGTGGTTGTAGGAGAATTG 6 0.15 No Hit GGGAAAAGGAACAGACAGATTCTGGTGGTAGAGGTGATAGCAGAAACCCT 6 0.15 No Hit AGTCGGTTATGGAATCAAGAAGCTACAAATCATGCTTACCATTGTAGACG 6 0.15 No Hit GGAATTCAAGCATGTTGAAGGGGGTACATCCTCAGACCTTGGCGTTAAAC 6 0.15 No Hit GGGGGTTGAGGATAATGTCACATCAAGATCACTACCGGATTTTGCAGTTA 6 0.15 No Hit AGATAATCTCAAAAATTGAAGCAGCAGCGGCGCCTTTGGGTTTTGATGTT 6 0.15 No Hit GGGATAGATAGCCACCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTT 6 0.15 No Hit GTTTTGATCGGTTGGCGTGGTGGTAGGGAGAATTGGAATTGGAGCTCCCA 6 0.15 No Hit TCTGGGTTGACCATAATTGACGCCTACGCTTGGCTGAAAACACCAGGGGA 6 0.15 No Hit TGCCAGGCACGTCCTTTCCCCAGGGAGCAGGTTGGAACGATAAAAGAAAT 6 0.15 No Hit TTTGTGTTTTGGTGGTGGGGAAGACATGGGAGGAAAATATATGAAACGGT 6 0.15 No Hit GCTGGTCTAGTTGACATGGCTGTCAAGACTATAACTGACGGCTGGCCTCT 6 0.15 No Hit GGTGGAGAGTAGATCATCTTTGAGATTGCTCATATCCGAGCTTTTAATGT 6 0.15 No Hit ACGAGGCAAGACTGTCTCAATCCAAGTACCTAGCCAGTGACAGCTTCACC 6 0.15 No Hit TGATAATGGAAATACAGAAACGAATTGTAAGGAAAAAGAGTAGGGCTTCA 6 0.15 No Hit TCTTTTCAAGCAGGAGAAGGAGAAGCTGATCATTCCCATCTCCTTAAGGA 6 0.15 No Hit CAAAGATATTGACGTCGGAGAGGGGAGCAGTCCTGTTAATAAGTTTCAGA 6 0.15 No Hit ATGTCTAGCACCTGCGACAACTGCGACTGCGCTGACAAGACCCAGTGTGT 6 0.15 No Hit CACCGATCCAACGTTTTTGTACTTTGGTCATGCTTTGAAGGAGGTCAAGT 6 0.15 No Hit GGAAGAACTGTTAAAAACAAAATCCGAGGCCACTTCAAAGGCCAAAGAGC 6 0.15 No Hit GCAAGACTTGTAGAAGGTATTGGACTGCAGGTGGGTCTTTAAGAAATGTT 5 0.125 No Hit GGGAGTATTTGCACTTGTGGTAACGGTATTTGCATTATTGATGGTTTTTA 5 0.125 No Hit GTCAATTCTGGTCCCTTCCTCCAAGAAGAGGTTATGTCTTTGAAAGGTTT 5 0.125 No Hit ATCACTTGGCCTGCAGAAAATAGCAAAGGCAAAAGGAAACTGAAAGAGAC 5 0.125 No Hit GAACTTTTCTCTCATGGAAATTTGCTGCGGCCTTGCAAAGGGATTTTGCT 5 0.125 No Hit AAGTCGGTCAAAGCATCGGTCAAAGCAAAACGCAAACTGAGAAGCCAGAA 5 0.125 No Hit AGTGCTCATACAAGACTTTAATTGTGCATTCCAGGAGAGTATTCTTCTTC 5 0.125 No Hit CACAAAACTTAACCAGACAGAGAAAGGGAGCTCACAAAAAGTTAAGAAAT 5 0.125 No Hit GGAGTACACTGCACCTTTTTCGAGTCCTACCCCTTCTCCTCTGAGGTCTG 5 0.125 No Hit TATTTTTGCTGCTGTTGGTTTTGCCGCGGGTTTGGTAGGGACTGCGCTTT 5 0.125 No Hit GCACCTCTGGCTTCTTTGCAAACTTTCTTAGTTAGTGGTGCAGTTTTTCT 5 0.125 No Hit GTCCAAAATTGCTACAAGATAAAGAAAGTTTCTTCGTTTGGGACAAAAAC 5 0.125 No Hit GGCTTCAAGTCGTTCGAAGAAAAAATCCAAGCAGAAACAAGAGAAGGGAG 5 0.125 No Hit GATGATCATGACGTGCCTGAAGAAACTAACCCTTTGGCTATTGTGCCCGA 5 0.125 No Hit GGCAAACCCTAAGGTCTACTTCGACATGACAATAGGCGGCGTCCCAGCCG 5 0.125 No Hit TGAAGAAAGCAGCACTCTTTGATGGTAGTTTATGTTCCTTTTTGTAGTTG 5 0.125 No Hit GACAGAAGACCATAGGCAACTTCATCTCCGTCAATCATTTCAGAGATTGA 5 0.125 No Hit AACAAAGGAAATTTCAGTTAGAGAGGAGAAAACAGGAGGATGAATTAAAA 5 0.125 No Hit GAGCAGCTAGAGTAGATGATGTGAATGGTATCAAGAGTTGCCTTGCAGAA 5 0.125 No Hit AATGCATAGAGATTTGAAGCCTGAGAATTTTTTATTTGCTAATAAGAAGG 5 0.125 No Hit GGCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATAGGAGGTTTC 5 0.125 No Hit CTATGATGGCTATTCTAGATGGCTTTCAGAAGGCATACTCCGTTGATTTT 5 0.125 No Hit TGTTGGCATTGATTGGAGAGCAGTTTGATCATGGAGATGAAATTTGTGGA 5 0.125 No Hit TGAGGCTGTAATCGAGGAGCAGAAGATGGGTGGAGGAAGTGCTTCTGATG 5 0.125 No Hit GGAGGAGCTAGGTCTCCGCCCAAGTGTTTCAATTGTCAACATGGTGGGAA 5 0.125 No Hit AAGCTATCATGGCCAGACACTATGTATCTGGTGGCCTCAAAGAAAAAGCT 5 0.125 No Hit AGAGCTTGTGAAGAAGGCAAAATTGGAAAAAATTGACGATCTTATGTTGC 5 0.125 No Hit CACCAATCGAAGATCCTATCCACCTTCAGTGCCACCGAATGTCATATAGA 5 0.125 No Hit GGGTGTCAAACTCTAATGGCAACAATTTTCTCCATAGCCTTGTTGGCAAT 5 0.125 No Hit CCAAATTCCTGCAACAGCAGTTATCGACAGCAACGAGGCAGCTCGACGCT 5 0.125 No Hit GTGTAGTGGAAGATTCCTCTTCAGCTAATCCAGAAATCCAGAAATCGAGC 5 0.125 No Hit AGCTTGTTGTCGGTGATGTGTTCAGAGAACCAGATTGCCCAAAGCTTCTC 5 0.125 No Hit CCCACCACCTCCACCAGTTCACAAGTCACCACCCCTACCACCCAAGAAAC 5 0.125 No Hit GATAGCATTGTTGCCTCTTTAATGCATTATGCCGAGATATCATTAAAGGG 5 0.125 No Hit GAGATACAGCGGCGAACAAGCTTCTAACTTCATCGGCAGTGAAAGCTAAT 5 0.125 No Hit GCATCTTGGCTGCCTCAAGACTGTTTCTTAATTTCCACCTCCACTCACAA 5 0.125 No Hit ACTGGGGTCTGAAGATAAATAGGTATCCTCTAGGAAGGTTTTGCCGGGGT 5 0.125 No Hit CCTCAACAGAAGATTCTTCTGCTTTCTACAAGGTTCCATTTTCCAGAGTA 5 0.125 No Hit TGTAACTAAGTGAGGCTCTCTCATTGGTTTATACTTCAATATAAGCCTTG 5 0.125 No Hit CCTGGCTTTGAGCCGGATAGGTCCTTGATCCAAAGCTGCCCGCCTAGTGC 5 0.125 No Hit TATGTTTTTAAATGGCTGTGGTTTCCACACTAAGGTAGCAAAATCATTAG 5 0.125 No Hit GTTTGATCATTTCGCGCCCTGTCAATTCTAGCTTATCTCATAAGAATGCT 5 0.125 No Hit GGAAGTTCCATTACATAGCAACTGGGATTGGCAATTGTTCTAGCCTTTTG 5 0.125 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0125 0.0 0.0 0.0 0.0 62-63 0.037500000000000006 0.0 0.0 0.0 0.0 64-65 0.05 0.0 0.0 0.0 0.0 66-67 0.05 0.0 0.0 0.0 0.0 68-69 0.05 0.0 0.0 0.0 0.0 70-71 0.1 0.0 0.0 0.0 0.0 72-73 0.15 0.0 0.0 0.0 0.0 74-75 0.15 0.0 0.0 0.0 0.0 76-77 0.21250000000000002 0.0 0.0 0.0 0.0 78-79 0.3375 0.0 0.0 0.0 0.0 80-81 0.5125 0.0 0.0 0.0 0.0 82-83 0.575 0.0 0.0 0.0 0.0 84-85 0.7625 0.0 0.0 0.0 0.0 86-87 0.875 0.0 0.0 0.0 0.0 88-89 1.0125 0.0 0.0 0.0 0.0 90-91 1.1875 0.0 0.0 0.0 0.0 92-93 1.3125 0.0 0.0 0.0 0.0 94-95 1.4125 0.0 0.0 0.0 0.0 96-97 1.525 0.0 0.0 0.0 0.0 98-99 1.7875 0.0 0.0 0.0 0.0 100-101 1.9875 0.0 0.0 0.0 0.0 102-103 2.3875 0.0 0.0 0.0 0.0 104-105 2.65 0.0 0.0 0.0 0.0 106-107 3.05 0.0 0.0 0.0 0.0 108-109 3.6125 0.0 0.0 0.0 0.0 110-111 4.0625 0.0 0.0 0.0 0.0 112-113 4.4375 0.0 0.0 0.0 0.0 114-115 4.75 0.0 0.0 0.0 0.0 116-117 5.25 0.0 0.0 0.0 0.0 118-119 5.525 0.0 0.0 0.0 0.0 120-121 5.8375 0.0 0.0 0.0 0.0 122-123 6.475 0.0 0.0 0.0 0.0 124-125 7.3375 0.0 0.0 0.0 0.0 126-127 7.9 0.0 0.0 0.0 0.0 128-129 8.5125 0.0 0.0 0.0 0.0 130-131 9.1875 0.0 0.0 0.0 0.0 132-133 10.325 0.0 0.0 0.0 0.0 134-135 11.2 0.0 0.0 0.0 0.0 136-137 12.375 0.0 0.0 0.0 0.0 138-139 13.412500000000001 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ACAAACG 10 0.006830828 145.0 6 GAAGTTT 10 0.006830828 145.0 5 TGGGATG 10 0.006830828 145.0 2 AAGTTTT 10 0.006830828 145.0 6 >>END_MODULE Read 1654170 spots for SRR26075359.sra Written 1654170 spots for SRR26075359.sra Read 1654170 spots for SRR26075359.sra Written 1654170 spots for SRR26075359.sra Read 1654170 spots for SRR26075359.sra Written 1654170 spots for SRR26075359.sra Read 1654170 spots for SRR26075359.sra Written 1654170 spots for SRR26075359.sra Read 1654170 spots for SRR26075359.sra Written 1654170 spots for SRR26075359.sra Read 1654170 spots for SRR26075359.sra Written 1654170 spots for SRR26075359.sra Read 1654170 spots for SRR26075359.sra Written 1654170 spots for SRR26075359.sra Read 1654170 spots for SRR26075359.sra Written 1654170 spots for SRR26075359.sra Read 1654170 spots for SRR26075359.sra Written 1654170 spots for SRR26075359.sra Read 1654170 spots for SRR26075359.sra Written 1654170 spots for SRR26075359.sra Read 1654170 spots for SRR26075359.sra Written 1654170 spots for SRR26075359.sra Read 1654170 spots for SRR26075359.sra Written 1654170 spots for SRR26075359.sra Read 1654170 spots for SRR26075359.sra Written 1654170 spots for SRR26075359.sra Read 1654170 spots for SRR26075359.sra Written 1654170 spots for SRR26075359.sra Read 1654170 spots for SRR26075359.sra Written 1654170 spots for SRR26075359.sra Read 1654170 spots for SRR26075359.sra Written 1654170 spots for SRR26075359.sra Read 1654170 spots for SRR26075359.sra Written 1654170 spots for SRR26075359.sra Read 1654172 spots for SRR26075359.sra Written 1654172 spots for SRR26075359.sra Read 1654170 spots for SRR26075359.sra Written 1654170 spots for SRR26075359.sra Read 1654170 spots for SRR26075359.sra Written 1654170 spots for SRR26075359.sra SRR ids: ['SRR26075359.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_0mmo7nk9 SRR26075359.sra spots: 33083402 blocks: [[1, 1654170], [1654171, 3308340], [3308341, 4962510], [4962511, 6616680], [6616681, 8270850], [8270851, 9925020], [9925021, 11579190], [11579191, 13233360], [13233361, 14887530], [14887531, 16541700], [16541701, 18195870], [18195871, 19850040], [19850041, 21504210], [21504211, 23158380], [23158381, 24812550], [24812551, 26466720], [26466721, 28120890], [28120891, 29775060], [29775061, 31429230], [31429231, 33083402]] SRR26075359 file size 12216611 SRR26075359 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o SRR26075359 SRR26075359_1.fastq SRR26075359_2.fastq Input file: SRR26075359_1.fastq Paired file: SRR26075359_2.fastq trimmed: SRR26075359-trimmed-pair1.fastq, SRR26075359-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Wed Feb 12 03:12:11 2025 >> started Wed Feb 12 03:12:50 2025 >> done (38.787s) 33083402 read pairs processed; of these: 198 ( 0.00%) short read pairs filtered out after trimming by size control 248081 ( 0.75%) empty read pairs filtered out after trimming by size control 32835123 (99.25%) read pairs available; of these: 6367721 (19.39%) trimmed read pairs available after processing 26467402 (80.61%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 18 8 0.00% 19 18 0.00% 20 23 0.00% 21 9 0.00% 22 21 0.00% 23 19 0.00% 24 26 0.00% 25 30 0.00% 26 30 0.00% 27 37 0.00% 28 42 0.00% 29 25 0.00% 30 50 0.00% 31 58 0.00% 32 33 0.00% 33 63 0.00% 34 74 0.00% 35 51 0.00% 36 54 0.00% 37 102 0.00% 38 109 0.00% 39 92 0.00% 40 110 0.00% 41 114 0.00% 42 113 0.00% 43 110 0.00% 44 167 0.00% 45 148 0.00% 46 172 0.00% 47 204 0.00% 48 229 0.00% 49 248 0.00% 50 301 0.00% 51 382 0.00% 52 389 0.00% 53 455 0.00% 54 482 0.00% 55 521 0.00% 56 492 0.00% 57 615 0.00% 58 834 0.00% 59 929 0.00% 60 1012 0.00% 61 1086 0.00% 62 1406 0.00% 63 1621 0.00% 64 1696 0.01% 65 2014 0.01% 66 2198 0.01% 67 2334 0.01% 68 2711 0.01% 69 3179 0.01% 70 3506 0.01% 71 4170 0.01% 72 4525 0.01% 73 5593 0.02% 74 6440 0.02% 75 7187 0.02% 76 8009 0.02% 77 8628 0.03% 78 9758 0.03% 79 10721 0.03% 80 11666 0.04% 81 13122 0.04% 82 14903 0.05% 83 16431 0.05% 84 18699 0.06% 85 20252 0.06% 86 21733 0.07% 87 23478 0.07% 88 25887 0.08% 89 27522 0.08% 90 28998 0.09% 91 30988 0.09% 92 33437 0.10% 93 35913 0.11% 94 38941 0.12% 95 42047 0.13% 96 44572 0.14% 97 47194 0.14% 98 49442 0.15% 99 50788 0.15% 100 52733 0.16% 101 55514 0.17% 102 57475 0.18% 103 59374 0.18% 104 62710 0.19% 105 66424 0.20% 106 68828 0.21% 107 72445 0.22% 108 74111 0.23% 109 75940 0.23% 110 76843 0.23% 111 79031 0.24% 112 82100 0.25% 113 84324 0.26% 114 87179 0.27% 115 91126 0.28% 116 93541 0.28% 117 96794 0.29% 118 99228 0.30% 119 100475 0.31% 120 101439 0.31% 121 103511 0.32% 122 105399 0.32% 123 107697 0.33% 124 111107 0.34% 125 112827 0.34% 126 116242 0.35% 127 120453 0.37% 128 122279 0.37% 129 123107 0.37% 130 126226 0.38% 131 126203 0.38% 132 127815 0.39% 133 128807 0.39% 134 131703 0.40% 135 134783 0.41% 136 135623 0.41% 137 138505 0.42% 138 140775 0.43% 139 143908 0.44% 140 145510 0.44% 141 145747 0.44% 142 148812 0.45% 143 150199 0.46% 144 149346 0.45% 145 152268 0.46% 146 154005 0.47% 147 156160 0.48% 148 157581 0.48% 149 159815 0.49% 150 161878 0.49% 151 26467402 80.61% 32835123 reads passed initial QC criterion=sequence-density sequence-density=0.24 sequence-density-rank=1 fanout-score=2.45 fanout-score-rank=30 prefix-density=0.24 prefix-fanout=2.4 sequence=GTCAGGGTACAT criterion=fanout-score sequence-density=0.03 sequence-density-rank=34 fanout-score=110.16 fanout-score-rank=1 prefix-density=0.34 prefix-fanout=10.2 sequence=CATCACCATCCCTGATTGATCTTTGATTCACAACAAGACCAACCAACCGTACAATGTTTACATAACTTGGGATAATCTCACAAGAAAGAAAGATCAATACAAAACTAGTCAGCTCAGGATGTTCCAGGCACGGTAGAACCGT criterion=sequence-density sequence-density=0.40 sequence-density-rank=1 fanout-score=2.51 fanout-score-rank=26 prefix-density=0.41 prefix-fanout=2.5 sequence=ATGTACCCTGAC criterion=fanout-score sequence-density=0.01 sequence-density-rank=33 fanout-score=344.91 fanout-score-rank=1 prefix-density=0.14 prefix-fanout=14.1 sequence=AGAGAAAAGATAAGCTAGGCAAGATGGTTTTACTA SRR26075359 testing PE reads STAR mapping to Ensembl genome Started job on | Feb 12 03:13:38 Started mapping on | Feb 12 03:13:38 Finished on | Feb 12 03:21:35 Mapping speed, Million of reads per hour | 247.81 Number of input reads | 32835123 Average input read length | 291 UNIQUE READS: Uniquely mapped reads number | 27755524 Uniquely mapped reads % | 84.53% Average mapped length | 290.38 Number of splices: Total | 25436978 Number of splices: Annotated (sjdb) | 24814306 Number of splices: GT/AG | 24978523 Number of splices: GC/AG | 354224 Number of splices: AT/AC | 24750 Number of splices: Non-canonical | 79481 Mismatch rate per base, % | 0.40% Deletion rate per base | 0.03% Deletion average length | 3.10 Insertion rate per base | 0.02% Insertion average length | 2.63 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 1064656 % of reads mapped to multiple loci | 3.24% Number of reads mapped to too many loci | 142876 % of reads mapped to too many loci | 0.44% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 11.41% % of reads unmapped: other | 0.38% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 4014943 4014943 4014943 N_multimapping 1064656 1064656 1064656 N_noFeature 783555 27355219 990139 N_ambiguous 337391 1838 142582 UnstrandedReadsAssigned:26634578 PositiveStrandReadsAssigned:398467 NegativeStrandReadsAssigned:26622803 Dataset is classified negative stranded MeadianReadLen=151 20thPercentileLength=150 echo kmer=145 SRR26075359 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,400 [index] number of k-mers: 62,057,036 [index] number of equivalence classes: 130,681 [quant] running in paired-end mode [quant] will process pair 1: SRR26075359-trimmed-pair1.fastq SRR26075359-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 32,835,123 reads, 27,193,930 reads pseudoaligned [quant] estimated average fragment length: 204.689 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,156 rounds 52401 SRR26075359.ke.tsv 34699 SRR26075359.se.tsv 87100 total ==> SRR26075359.ke.tsv <== target_id length eff_length est_counts tpm Potri.005G200100.1.v4.1 2018 1814.31 2639 44.5832 Potri.005G024800.1.v4.1 1035 831.311 1397 51.5082 Potri.004G059700.1.v4.1 961 757.311 16 0.647574 Potri.007G009000.2.v4.1 1416 1212.31 0 0 Potri.003G141000.2.v4.1 2943 2739.31 1050 11.7487 Potri.016G087400.1.v4.1 270 94.6453 3756.19 1216.44 Potri.015G069301.1.v4.1 564 361.799 0 0 Potri.010G195200.1.v4.1 1773 1569.31 405 7.91024 Potri.012G127500.1.v4.1 977 773.311 14920 591.368 ==> SRR26075359.se.tsv <== Potri.001G166300.v4.1 0 Potri.001G448400.v4.1 265 Potri.001G233950.v4.1 0 Potri.001G122700.v4.1 919 Potri.001G212900.v4.1 3 Potri.001G182400.v4.1 0 Potri.001G256600.v4.1 0 Potri.001G040500.v4.1 17 Potri.001G416900.v4.1 0 Potri.001G452600.v4.1 1386 SRR26075359 completed mapping pipeline successfully