Starting /dee2/code/volunteer_pipeline.sh SRR26075360 current disk space = 3048885354496 free memory = 1444787588 SRR26075360 SRAfilesize be479fc925bdc35cd8f0fcc905b438e8 SRR26075360.sra SRR26075360.sra file validated SRR26075360 is paired end SRR26075360 is conventional basespace SRR26075360 read1 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR26075360_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.58075 37.0 37.0 37.0 37.0 37.0 2 36.6295 37.0 37.0 37.0 37.0 37.0 3 36.6855 37.0 37.0 37.0 37.0 37.0 4 36.6645 37.0 37.0 37.0 37.0 37.0 5 36.7145 37.0 37.0 37.0 37.0 37.0 6 36.71 37.0 37.0 37.0 37.0 37.0 7 36.599 37.0 37.0 37.0 37.0 37.0 8 36.6795 37.0 37.0 37.0 37.0 37.0 9 36.6825 37.0 37.0 37.0 37.0 37.0 10-14 36.64865 37.0 37.0 37.0 37.0 37.0 15-19 36.5969 37.0 37.0 37.0 37.0 37.0 20-24 36.5621 37.0 37.0 37.0 37.0 37.0 25-29 36.482600000000005 37.0 37.0 37.0 37.0 37.0 30-34 36.4601 37.0 37.0 37.0 37.0 37.0 35-39 36.4208 37.0 37.0 37.0 37.0 37.0 40-44 36.392900000000004 37.0 37.0 37.0 37.0 37.0 45-49 36.340599999999995 37.0 37.0 37.0 37.0 37.0 50-54 36.2811 37.0 37.0 37.0 37.0 37.0 55-59 36.270599999999995 37.0 37.0 37.0 37.0 37.0 60-64 36.2043 37.0 37.0 37.0 37.0 37.0 65-69 36.077999999999996 37.0 37.0 37.0 37.0 37.0 70-74 36.109899999999996 37.0 37.0 37.0 37.0 37.0 75-79 36.1905 37.0 37.0 37.0 37.0 37.0 80-84 36.136 37.0 37.0 37.0 37.0 37.0 85-89 35.967200000000005 37.0 37.0 37.0 37.0 37.0 90-94 35.9678 37.0 37.0 37.0 37.0 37.0 95-99 35.9983 37.0 37.0 37.0 37.0 37.0 100-104 35.8883 37.0 37.0 37.0 37.0 37.0 105-109 35.806599999999996 37.0 37.0 37.0 37.0 37.0 110-114 35.681999999999995 37.0 37.0 37.0 37.0 37.0 115-119 35.6537 37.0 37.0 37.0 37.0 37.0 120-124 35.67479999999999 37.0 37.0 37.0 37.0 37.0 125-129 35.5351 37.0 37.0 37.0 37.0 37.0 130-134 35.3966 37.0 37.0 37.0 34.6 37.0 135-139 35.1939 37.0 37.0 37.0 32.2 37.0 140-144 35.102199999999996 37.0 37.0 37.0 29.8 37.0 145-149 34.9292 37.0 37.0 37.0 27.4 37.0 150-151 34.572 37.0 37.0 37.0 25.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.0 1101 2 0.0 1101 3 0.0 1101 4 0.0 1101 5 0.0 1101 6 0.0 1101 7 0.0 1101 8 0.0 1101 9 0.0 1101 10-14 0.0 1101 15-19 0.0 1101 20-24 0.0 1101 25-29 0.0 1101 30-34 0.0 1101 35-39 0.0 1101 40-44 0.0 1101 45-49 0.0 1101 50-54 0.0 1101 55-59 0.0 1101 60-64 0.0 1101 65-69 0.0 1101 70-74 0.0 1101 75-79 0.0 1101 80-84 0.0 1101 85-89 0.0 1101 90-94 0.0 1101 95-99 0.0 1101 100-104 0.0 1101 105-109 0.0 1101 110-114 0.0 1101 115-119 0.0 1101 120-124 0.0 1101 125-129 0.0 1101 130-134 0.0 1101 135-139 0.0 1101 140-144 0.0 1101 145-149 0.0 1101 150-151 0.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 17 1.0 18 0.0 19 1.0 20 1.0 21 0.0 22 0.0 23 2.0 24 0.0 25 5.0 26 6.0 27 13.0 28 17.0 29 24.0 30 22.0 31 35.0 32 72.0 33 114.0 34 173.0 35 460.0 36 2882.0 37 172.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.766725131545975 14.282134803307441 7.717364069155599 37.23377599599098 2 19.425 13.900000000000002 34.8 31.874999999999996 3 17.175 16.45 28.449999999999996 37.925 4 23.025000000000002 24.4 22.400000000000002 30.175 5 25.7 28.075 23.75 22.475 6 24.55 32.300000000000004 21.55 21.6 7 16.025 30.125 37.574999999999996 16.275000000000002 8 17.675 25.724999999999998 33.225 23.375 9 18.6 22.925 35.375 23.1 10-14 20.356017800890044 28.921446072303613 27.561378068903448 23.161158057902895 15-19 20.36 26.82 27.72 25.1 20-24 21.185000000000002 27.224999999999998 27.55 24.04 25-29 21.2 27.785 27.060000000000002 23.955000000000002 30-34 21.26 27.105 27.735 23.9 35-39 19.785 27.99 27.33 24.895 40-44 21.385 28.915000000000003 26.645000000000003 23.055 45-49 21.32 27.42 27.04 24.22 50-54 21.915000000000003 28.725 26.35 23.01 55-59 21.335 27.66 27.075 23.93 60-64 21.66 26.13 28.29 23.919999999999998 65-69 21.529999999999998 27.12 28.199999999999996 23.150000000000002 70-74 21.015 27.334999999999997 27.76 23.89 75-79 21.895 26.645000000000003 27.445000000000004 24.015 80-84 20.955 26.575 27.735 24.735 85-89 20.925 26.435 28.535 24.104999999999997 90-94 21.43 26.82 27.925 23.825 95-99 22.12 27.13 27.115000000000002 23.635 100-104 21.795 27.35 27.27 23.585 105-109 21.085 28.015 26.555 24.345 110-114 22.24 26.895000000000003 26.889999999999997 23.974999999999998 115-119 22.71 27.195000000000004 26.135 23.96 120-124 21.855 28.470000000000002 25.885 23.79 125-129 21.88 27.43 26.02 24.67 130-134 21.65 27.310000000000002 26.55 24.490000000000002 135-139 22.2 27.474999999999998 25.825 24.5 140-144 22.84 26.545 26.619999999999997 23.995 145-149 23.965 26.334999999999997 26.3 23.400000000000002 150-151 24.0 26.787499999999998 25.137500000000003 24.075 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.5 18 0.5 19 0.5 20 0.5 21 0.0 22 1.0 23 1.5 24 0.5 25 1.5 26 2.0 27 2.0 28 4.0 29 11.0 30 12.0 31 14.0 32 34.5 33 34.5 34 37.5 35 55.5 36 68.0 37 81.0 38 105.5 39 124.0 40 143.0 41 198.5 42 250.0 43 255.0 44 261.0 45 261.0 46 241.5 47 260.0 48 243.0 49 211.0 50 210.0 51 166.5 52 123.0 53 104.5 54 89.5 55 74.0 56 50.5 57 45.0 58 40.0 59 34.0 60 26.5 61 18.5 62 18.0 63 17.0 64 13.5 65 9.0 66 8.0 67 7.0 68 6.0 69 2.5 70 0.5 71 4.0 72 4.0 73 2.5 74 4.0 75 3.0 76 1.5 77 1.0 78 0.5 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.22499999999999998 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.005 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150-151 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 62.050000000000004 #Duplication Level Percentage of deduplicated Percentage of total 1 64.62530217566479 40.1 2 21.112006446414185 26.200000000000003 3 8.178887993553586 15.225 4 3.30378726833199 8.200000000000001 5 1.4907332796132151 4.625 6 0.604351329572925 2.25 7 0.32232070910556004 1.4000000000000001 8 0.201450443190975 1.0 9 0.08058017727639001 0.44999999999999996 >10 0.08058017727639001 0.5499999999999999 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CCTGTGAAAACATACCTGAACATGGGGTCTGCGAAAACTGGAGTTAGAAG 12 0.3 No Hit CCCAGAAGCAGCAGATCAAACTAAAACTCCTTAATGGTTTTTTCTTTTGT 10 0.25 No Hit GCACTAACAGTACCCTCAACAGCTCCAACGGGAGATCTCAAAGGCCCGGA 9 0.22499999999999998 No Hit CCCAAACTTACTCTGCACCAGGCAGCAGTAATCGTATCGATATGCGAGCC 9 0.22499999999999998 No Hit ATTTCAAATCATTCATTTATTTTATTTTGCTGAGAGCACTTAACAGGTAA 8 0.2 No Hit CTGTGCTCCTGACAGTGTATAGGAATCCAGTAAACTGCGAAGAATTTCAA 8 0.2 No Hit GGCTTGGATTCTTGCAAAATTTCCCATTGACAAGCACAGCAGAATCAGTA 8 0.2 No Hit ATCATCCAGCTGCTTGTCCTTCATTCGCAACTCTGGCCTCTTGATCAACT 8 0.2 No Hit CAACAGTATTTGGGAGTGATGCAGTGGACTGTCCTGTTCGGCCACCATCC 8 0.2 No Hit CAGCGGATCGCTCTCATTTACAAGTGCAAGGATCGCAGGTACAGTTGGCT 7 0.17500000000000002 No Hit ATCTAAAGATCTTTATACTTGTTCGATGGTGATTTCTGATAAGCCTCCAC 7 0.17500000000000002 No Hit GTAGATTTTGGATCAAGACCCTGGAGAAAACGTCTGAGAGGCCTAGGTGG 7 0.17500000000000002 No Hit TGGTAATTTGCAAGGAATGCTGCACAACCTCCGGCCTTATAATTGAATAC 7 0.17500000000000002 No Hit CCCGTTTATCAGACCACCAAGCAGACCAAATTGTCCAGCTCCATCAACCG 7 0.17500000000000002 No Hit GTCGAGTCCGAAGTCGCCGTCGAAAGCGTCACCTTCAGGCAAAGGTGCGG 7 0.17500000000000002 No Hit CCTCTTTTTTCCATGCTTCACGACACAAACAAAACATCTTTACCCAGGAG 7 0.17500000000000002 No Hit GTAAGGCTTCGTGATGATTACACACGACAGGAACCATCAGCTTCAGATGA 7 0.17500000000000002 No Hit TGTCTCTGCTCCAATATGGATAGGCAGAAAGCTCCCATCAGGTTGCTGGA 6 0.15 No Hit GTTAGGTGGAGCAAATTGGTAGAGGGTCCTCTGTTGCAGCAGATTCTGGT 6 0.15 No Hit TGAGAATCCAAAACTTCCTGGACTTTGCAACATCTCTAGGGTAAAAGGCA 6 0.15 No Hit GCCTACTTAAAAAAGCTTCAGCCAAGTATCCAGAGATTCCTTTGTGGCCA 6 0.15 No Hit GCCTCCTGGGCCCGGATGTAATCTGAATCAGGCTCTGGTTTTTCTGAAGG 6 0.15 No Hit CAGACCTTCTTGTTGTATATGTTGAAAACAACATTCAAAGCCCACCAAGT 6 0.15 No Hit GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGCACGAATCTCGGTT 6 0.15 TruSeq Adapter, Index 27 (97% over 37bp) GCTAGCGCCGAGTCTGAGATGTCACACTGGATGTACTCAACTGGGCAGTT 6 0.15 No Hit GCTCCTCCAAACATTGCTACGCGCCGACACTGGGGTTGTAGGGGTCACTG 6 0.15 No Hit GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGCACGAATCTCGGGT 6 0.15 TruSeq Adapter, Index 27 (97% over 37bp) AACCCCACATCCACTCAAACTTCTGTGTAGTTTTGGCAGATAAAACTGAA 6 0.15 No Hit CACATGTTCTTCCCCATCTCCATCTTAAAGTAACCGTTGTCGCCCCAGTC 6 0.15 No Hit GGGTTGTACGCTCAGGGACTAACCTTGCTGCGATAAAGCTTGCCAAAGAC 6 0.15 No Hit ATAGAGAGGGGAAGTGCTCAAGTCACCATATACTACTCCAAGGCTCTGAT 6 0.15 No Hit AAGGATTCTTTGACAGATTTTCCAACCCAGAGATCTCCTTGTCAACTATG 6 0.15 No Hit CAATTCTTTGCCCTCCAGGATGTATCCATCAGCTCGCCCACACTGACCAG 5 0.125 No Hit GTTTTTTCTCGAGAAGATTACGTAGTTCATCTTCTTGGATACATTCTTCA 5 0.125 No Hit ATCGGAAACATGTCGCCGCCACCATCCTGATCCTGATGATCATCATCATC 5 0.125 No Hit GTTCCAAGTACCTTCGCAATTTTGACAAGCTGATCATGGTTGTCATGGCC 5 0.125 No Hit CTGGGCTGATGGCTCTCCATCAAACATGCCGAGTCTCATTTGGACAGTTA 5 0.125 No Hit TGGGGGTTATGCATCATCATCTTCTTCATCTTCTTGCAGATCTGAAAGGG 5 0.125 No Hit GGCAAATTCGCAAGCGAAGGCGAAGAAGAAGTCGAACCAAACGACGACGT 5 0.125 No Hit CTATATATTAGGATGGCACTAAATGCTGTAAACATTATGAACTCAAAGTG 5 0.125 No Hit GTCATCGTCAAATGTCAGCAAGTCTTGGGGCTCTCCCAGCTCCTTTTCCC 5 0.125 No Hit GTCACGGCTGCTCTGTTCAGAATCCCTTCTGCTTTCGCTATCAGTGTACC 5 0.125 No Hit CTGTACTGCCCTCCTCGTTAAGTGACTTCTGGAAGTAGTGCATCTTTTGC 5 0.125 No Hit CCTGCGGTGCCCATCCACACATAATTCCTCGTCCTTCTATGCGTTCCAAG 5 0.125 No Hit CACGCTATGACTGATTGAACACGGAGTGAAGCCTGCCATCTTCATTCTTG 5 0.125 No Hit CGGACGTCCTTTCCAGTAACGGTATGACCGTTGGCAGTGAACTTCTGCTT 5 0.125 No Hit CGCACAAGCAGCTATGGTAGACTCAATGATTGCCAACTCCGACGCAGAAA 5 0.125 No Hit ACAAGAGTGAGGAGGCCAAGGACTTTAGCCTTCTCTTTCTTTGGTGCATA 5 0.125 No Hit AGTGGAAGCTCAAATCCATATTTCTTGGGATCACTGAATAAAGAATATTT 5 0.125 No Hit CAGCCAACAAAGCAGTGACGCGTACACAAGACAAAGGATTTATAGGAACC 5 0.125 No Hit GGATGATAATGTGGAGGGTTTAAGCCGCCATTTGGTTCAAAGTCTATACG 5 0.125 No Hit CTGAGTTGTTGCTGCCGCTGGAGGCTGTTCGGCGGCTGAGGAAGGAAAGA 5 0.125 No Hit CGAGAGCTTTCTTGATACCTACTTTACGTCCATCTGATGTGATAATTTTA 5 0.125 No Hit GATCGTCATACTCATCATCTTCTCCCAACTCTTCCTCTGCAAGTGCAGGT 5 0.125 No Hit CCTCATTTCACCAGATTTTAGAGACATCACATAGTATTATACATCAAGTC 5 0.125 No Hit CTCCACGGTTTGAATATCCATCGCCTCCACGGTTTGAATATCCACCGCCT 5 0.125 No Hit GCAGGATCAGTCTCGCCACGGAGGCCATAGTTGAAGGCATGAAGATTTGC 5 0.125 No Hit TTCCGCGTTCGCTCGTCACTACTAGCGGAATCTCGGTTGATGTCTTTTCC 5 0.125 No Hit CCCGTACATCTTAACTCCACCATCAGAACCCTGTGACCATACAGTAAACT 5 0.125 No Hit CAGGCTGAAAAAATGCCGCCTTGGGACATGATACAAGAGATGGAGGTCCA 5 0.125 No Hit TGGGATCTGAAAAGGAAGTCCTCTAATTCCAGTACTTAATAGCAGGAAAA 5 0.125 No Hit ATCTTCTATCATGCCCTTGTTTTCCATGTTCACCTCCATCATAGTAAACC 5 0.125 No Hit CTCCGATATCGCTTCTATAACAAACTTTGCCGGATCCACACACTCAGCTA 5 0.125 No Hit GGTACCTAAACAAAAGCATTTATTTCACATCTAACCAAAGGAACCAAGAT 5 0.125 No Hit TGACATGTGAAGTACAGCCGAACACATTATTACCAGAAATGTCATGGCTC 5 0.125 No Hit GGGACAAAATCCTATTGTAAACAAGTCTCAACATACATCCAAAAAGTTCT 5 0.125 No Hit ATACAGATGGTGAAGTACTCACGAAATAGAATGGTAAGACTTACTAAAAC 5 0.125 No Hit GCTCCTGTCAAAGCACCTCCAAGCATGGCATTCTTCCAGTCATGAGTGCC 5 0.125 No Hit CACCTTCACAACAGCAACCAAGCAAATTTACCAATCTATTGTTCCGTAGC 5 0.125 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0125 0.0 0.0 0.0 0.0 62-63 0.025 0.0 0.0 0.0 0.0 64-65 0.075 0.0 0.0 0.0 0.0 66-67 0.0875 0.0 0.0 0.0 0.0 68-69 0.1 0.0 0.0 0.0 0.0 70-71 0.1125 0.0 0.0 0.0 0.0 72-73 0.125 0.0 0.0 0.0 0.0 74-75 0.125 0.0 0.0 0.0 0.0 76-77 0.125 0.0 0.0 0.0 0.0 78-79 0.175 0.0 0.0 0.0 0.0 80-81 0.2375 0.0 0.0 0.0 0.0 82-83 0.30000000000000004 0.0 0.0 0.0 0.0 84-85 0.36250000000000004 0.0 0.0 0.0 0.0 86-87 0.5249999999999999 0.0 0.0 0.0 0.0 88-89 0.7 0.0 0.0 0.0 0.0 90-91 0.9125 0.0 0.0 0.0 0.0 92-93 1.1124999999999998 0.0 0.0 0.0 0.0 94-95 1.3625 0.0 0.0 0.0 0.0 96-97 1.575 0.0 0.0 0.0 0.0 98-99 2.0375 0.0 0.0 0.0 0.0 100-101 2.4625000000000004 0.0 0.0 0.0 0.0 102-103 2.75 0.0 0.0 0.0 0.0 104-105 2.9749999999999996 0.0 0.0 0.0 0.0 106-107 3.375 0.0 0.0 0.0 0.0 108-109 3.725 0.0 0.0 0.0 0.0 110-111 4.0375 0.0 0.0 0.0 0.0 112-113 4.5125 0.0 0.0 0.0 0.0 114-115 4.9125 0.0 0.0 0.0 0.0 116-117 5.4375 0.0 0.0 0.0 0.0 118-119 5.824999999999999 0.0 0.0 0.0 0.0 120-121 6.175000000000001 0.0 0.0 0.0 0.0 122-123 6.4375 0.0 0.0 0.0 0.0 124-125 7.0625 0.0 0.0 0.0 0.0 126-127 7.612500000000001 0.0 0.0 0.0 0.0 128-129 8.15 0.0 0.0 0.0 0.0 130-131 8.725 0.0 0.0 0.0 0.0 132-133 9.1 0.0 0.0 0.0 0.0 134-135 9.7 0.0 0.0 0.0 0.0 136-137 10.3 0.0 0.0 0.0 0.0 138-139 11.4375 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GCCAGAT 10 0.006830828 145.0 1 TCAGTGC 10 0.006830828 145.0 145 >>END_MODULE SRR26075360 read2 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR26075360_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.33525 37.0 37.0 37.0 37.0 37.0 2 36.3235 37.0 37.0 37.0 37.0 37.0 3 36.351 37.0 37.0 37.0 37.0 37.0 4 36.42 37.0 37.0 37.0 37.0 37.0 5 36.412 37.0 37.0 37.0 37.0 37.0 6 36.3205 37.0 37.0 37.0 37.0 37.0 7 36.303 37.0 37.0 37.0 37.0 37.0 8 36.3695 37.0 37.0 37.0 37.0 37.0 9 36.3635 37.0 37.0 37.0 37.0 37.0 10-14 36.3168 37.0 37.0 37.0 37.0 37.0 15-19 36.2664 37.0 37.0 37.0 37.0 37.0 20-24 36.2332 37.0 37.0 37.0 37.0 37.0 25-29 36.128499999999995 37.0 37.0 37.0 37.0 37.0 30-34 35.9842 37.0 37.0 37.0 37.0 37.0 35-39 35.952600000000004 37.0 37.0 37.0 37.0 37.0 40-44 35.8578 37.0 37.0 37.0 37.0 37.0 45-49 35.8471 37.0 37.0 37.0 37.0 37.0 50-54 35.7356 37.0 37.0 37.0 37.0 37.0 55-59 35.7029 37.0 37.0 37.0 37.0 37.0 60-64 35.7859 37.0 37.0 37.0 37.0 37.0 65-69 35.67450000000001 37.0 37.0 37.0 37.0 37.0 70-74 35.63250000000001 37.0 37.0 37.0 37.0 37.0 75-79 35.597500000000004 37.0 37.0 37.0 37.0 37.0 80-84 35.6502 37.0 37.0 37.0 37.0 37.0 85-89 35.575100000000006 37.0 37.0 37.0 37.0 37.0 90-94 35.4859 37.0 37.0 37.0 37.0 37.0 95-99 35.58 37.0 37.0 37.0 37.0 37.0 100-104 35.544399999999996 37.0 37.0 37.0 37.0 37.0 105-109 35.4491 37.0 37.0 37.0 34.6 37.0 110-114 35.4319 37.0 37.0 37.0 37.0 37.0 115-119 35.4317 37.0 37.0 37.0 37.0 37.0 120-124 35.3135 37.0 37.0 37.0 34.6 37.0 125-129 35.2273 37.0 37.0 37.0 34.6 37.0 130-134 35.2491 37.0 37.0 37.0 34.6 37.0 135-139 35.09475 37.0 37.0 37.0 27.4 37.0 140-144 35.12305 37.0 37.0 37.0 27.4 37.0 145-149 34.987649999999995 37.0 37.0 37.0 25.0 37.0 150-151 34.611625000000004 37.0 37.0 37.0 25.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.0 1101 2 0.0 1101 3 0.0 1101 4 0.0 1101 5 0.0 1101 6 0.0 1101 7 0.0 1101 8 0.0 1101 9 0.0 1101 10-14 0.0 1101 15-19 0.0 1101 20-24 0.0 1101 25-29 0.0 1101 30-34 0.0 1101 35-39 0.0 1101 40-44 0.0 1101 45-49 0.0 1101 50-54 0.0 1101 55-59 0.0 1101 60-64 0.0 1101 65-69 0.0 1101 70-74 0.0 1101 75-79 0.0 1101 80-84 0.0 1101 85-89 0.0 1101 90-94 0.0 1101 95-99 0.0 1101 100-104 0.0 1101 105-109 0.0 1101 110-114 0.0 1101 115-119 0.0 1101 120-124 0.0 1101 125-129 0.0 1101 130-134 0.0 1101 135-139 0.0 1101 140-144 0.0 1101 145-149 0.0 1101 150-151 0.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 13 2.0 14 4.0 15 9.0 16 13.0 17 3.0 18 6.0 19 2.0 20 3.0 21 5.0 22 10.0 23 8.0 24 12.0 25 10.0 26 11.0 27 10.0 28 10.0 29 14.0 30 30.0 31 38.0 32 45.0 33 88.0 34 204.0 35 654.0 36 2577.0 37 232.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 41.96049012253063 20.280070017504375 11.0527631907977 26.70667666916729 2 27.250000000000004 27.200000000000003 29.9 15.65 3 22.825 28.349999999999998 30.375000000000004 18.45 4 25.4 31.2 22.5 20.9 5 26.575 36.3 21.375 15.75 6 23.974999999999998 35.55 22.775000000000002 17.7 7 22.875 21.25 37.2 18.675 8 19.75 27.6 25.775 26.875 9 22.875 26.05 28.499999999999996 22.575 10-14 23.925 28.465 25.245 22.365 15-19 25.295 28.050000000000004 25.88 20.775 20-24 24.92 28.43 25.119999999999997 21.529999999999998 25-29 24.365000000000002 27.66 26.515 21.46 30-34 24.740000000000002 28.005000000000003 26.0 21.255 35-39 24.63 27.560000000000002 26.68 21.13 40-44 25.019999999999996 27.71 25.674999999999997 21.595 45-49 24.64 27.88 26.279999999999998 21.2 50-54 23.615 27.894999999999996 26.825 21.665 55-59 24.67 27.83 26.314999999999998 21.185000000000002 60-64 25.155 27.625 26.345000000000002 20.875 65-69 24.705 27.694999999999997 26.8 20.8 70-74 24.4 28.27 25.955000000000002 21.375 75-79 24.85 28.194999999999997 26.375 20.580000000000002 80-84 25.080000000000002 27.52 26.290000000000003 21.11 85-89 24.745 27.85 26.21 21.195 90-94 25.285000000000004 27.515 26.44 20.76 95-99 25.564999999999998 27.595 26.11 20.73 100-104 24.98 27.52 26.290000000000003 21.21 105-109 24.635 27.825 26.340000000000003 21.2 110-114 25.335 27.815 25.83 21.02 115-119 26.05 28.93 24.59 20.43 120-124 25.240000000000002 28.335 26.095000000000002 20.330000000000002 125-129 25.86 27.224999999999998 26.419999999999998 20.495 130-134 25.900000000000002 27.815 26.150000000000002 20.135 135-139 26.696334816740837 27.416370818540926 25.716285814290714 20.171008550427523 140-144 26.563984597689654 27.999199879981994 25.908886332949944 19.52792918937841 145-149 27.336834208552137 28.71717929482371 24.4161040260065 19.529882470617654 150-151 28.078509813726715 27.165895736967123 25.528191023877984 19.22740342542818 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 1.0 7 1.5 8 0.5 9 0.0 10 0.0 11 0.0 12 0.5 13 1.0 14 0.5 15 0.0 16 0.0 17 0.5 18 0.5 19 0.5 20 0.5 21 0.0 22 0.5 23 1.5 24 9.5 25 10.5 26 4.0 27 2.0 28 1.5 29 2.0 30 3.5 31 6.5 32 12.0 33 21.5 34 25.5 35 28.5 36 47.0 37 74.5 38 105.0 39 137.5 40 172.0 41 227.0 42 259.0 43 257.0 44 258.0 45 261.0 46 293.0 47 291.5 48 246.0 49 205.5 50 160.0 51 136.5 52 120.5 53 102.0 54 88.5 55 69.5 56 52.0 57 39.0 58 40.0 59 40.5 60 29.0 61 25.5 62 20.0 63 13.0 64 14.0 65 11.5 66 5.0 67 3.5 68 4.0 69 3.5 70 3.5 71 2.0 72 0.5 73 0.5 74 0.5 75 2.0 76 2.0 77 1.5 78 1.5 79 1.0 80 1.0 81 0.5 82 0.5 83 2.5 84 2.0 85 0.0 86 1.0 87 2.5 88 2.5 89 4.0 90 3.5 91 0.5 92 0.5 93 1.5 94 2.0 95 1.0 96 0.0 97 0.0 98 0.0 99 0.0 100 6.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.025 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.005 140-144 0.015 145-149 0.025 150-151 0.0125 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 62.375 #Duplication Level Percentage of deduplicated Percentage of total 1 65.53106212424849 40.875 2 20.841683366733466 26.0 3 7.5350701402805615 14.099999999999998 4 3.2865731462925853 8.200000000000001 5 1.5230460921843687 4.75 6 0.6412825651302605 2.4 7 0.2004008016032064 0.8750000000000001 8 0.2004008016032064 1.0 9 0.08016032064128256 0.44999999999999996 >10 0.16032064128256512 1.35 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG 17 0.42500000000000004 No Hit GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGT 15 0.375 No Hit GAAATTTGATATTTAAATACAGTAATTATCAGAATTATTAATATATATTT 12 0.3 No Hit GAATTTGATTCATGAAATCTTCTTTGCTGGAGAAGAGAAGGTTGAAGGAG 10 0.25 No Hit TGGTGTCCAGAGAGGCAAGGATGCAAGCAACCTGACGTAGTGGGCTTAGC 9 0.22499999999999998 No Hit ATCAGAAACAAATAAGTAGACAAATCTTGATGCGGATGATGGAGGTAGAG 9 0.22499999999999998 No Hit GATCCAAACCTTCTGCGTTCTTATGTTGTTCGGCAGGAAGGAATCCCGCT 8 0.2 No Hit AATCCTAGAAAAAGAGAGAACGATATGAAAGTTGCTAATTTGCTAGGAGC 8 0.2 No Hit GATGGGACTCGTCTCCTAACCGGCAGCTCATTCATGAGATTATTGATGCT 8 0.2 No Hit AGCAGAATGAGTTATTAACAAGCGCTTATAATTCAATGTCACATGAATTG 8 0.2 No Hit ATGGACTCTTTGAGGTCAAGGAGGACCATCACTGAGACTTTTGTATTGGA 8 0.2 No Hit GGTGCTTGGAATTAGTGGTTTCTGGGTGCAAAATTAGTGCCGGCAGCGAT 7 0.17500000000000002 No Hit AGTTCCTCACCGACCGGCTGAAGACATGGCATTTTCGGTTGCAAGGTTCA 7 0.17500000000000002 No Hit GCTGGTCTGTTCGTAGCTGGTCTACACAACGACTCCTCACCCACCGCCGC 7 0.17500000000000002 No Hit AGAACACCTGCCTTGATGGGTTTGCTGGCCATCTAATGGATGGAAATGTA 7 0.17500000000000002 No Hit GCAGTGACTTGGAGCCAGGGAATGAGGAAGATGATCCTGAAGGGGAGGAA 7 0.17500000000000002 No Hit GGAAGCTTCACCATCAAATTCCCAAAAAGAAATCTCGAGATATCGAATAA 6 0.15 No Hit GTTGAGTCTTTTGCGTTGTCAAGGCCTCAAAAGAGGACCATGATCACATG 6 0.15 No Hit GTTCCTGCTGCTCGCGGCACCGGCAGCAGGTCATTGCCGGGCCACAACAA 6 0.15 No Hit AGATGGTTTTACTAGACAAGATGTGGGATGATGTTGTTGCTGGACCTCAG 6 0.15 No Hit ATTAGGAACCCTAATGAGAAGCCGTTAGTGATATTGGAGACACCAAAGCC 6 0.15 No Hit CGTTCACACGGTGAACCTGCTGCTACTCATCATGGTATTCATCACCATTC 6 0.15 No Hit GGGGGCCAAGGAAGCGTGGCCTTGATAGTAGGAGTAACGGGAATTGTCGG 6 0.15 No Hit TGAAGATCTCGCCCACGATAGTCCTGGTGATAAGTCTCTGCTTCATTGGC 6 0.15 No Hit TAGTAATACGTGCTACTCAACCTTTGAGGAAGATGATCTACCGCTAGCTT 6 0.15 No Hit GTGGGTGTGGCTCTGGCTGCAAGTGCGGCAGCGGCTGTGGAGGATGCAAG 6 0.15 No Hit CGGAGATAAAGCTGAATTGAACAGTGGACAGTTCTATGGGTTTTGTGGCT 6 0.15 No Hit CTTGCAATGATGCCTCATCCTGAGCGTTGCTTCTTGATGTGGCAATTCCC 6 0.15 No Hit ATGGTGTCAACAAGTGGGGTCCTCCTAAACTGAGGATGTTTGGGGTGACA 6 0.15 No Hit CCGGATGGAGAGGTGGATGATGGAGATGGATCTTTTATGGTTTTGAAGTC 6 0.15 No Hit ATTCAGGCTCTCAGAGCCAAGGGGCTTGAAGAAGATCCTCGGAACTACTT 6 0.15 No Hit GTTTCCGTCTGTACAAGGAAGGAGTTTACACCACCAGCACTTGTGGCAGT 6 0.15 No Hit CGAGAATAGAGAAAGCCCGCGTGTTTGTTTACTCTAAGAGAAAGCACAAA 5 0.125 No Hit GGAAGGAAGAGCATTTTGTTATTGTCTCTTTAAGCGGCTGGGGTGTACTC 5 0.125 No Hit CCATGATCGGGATAGAGAGAGGGACAGGGTTCGGGATAGGGATCAGGAGA 5 0.125 No Hit GTCCTTAAGAAGATGGAGGATGATATGTATCGGAGAGCCATGTTTGATGC 5 0.125 No Hit TGGAAGTTGGGGACTCTGCCACCTGGCCTCATTACTTTTTGGAAACGGAC 5 0.125 No Hit ACCTGTCTTATCCTCCGTGGCCCCGTCCCCCGATGCCAGTAGGCATGGAC 5 0.125 No Hit GGAAGGTTGAACAAATGTGGGGTTATCAGTCCTCGTTTTGATGTTGGTGT 5 0.125 No Hit GTTCAAGTTAGATTCCAAAAGGGATTTTGTTTTGGCTTTGTGGAGTTTGA 5 0.125 No Hit AGAAAGAATCAATATGCCAAGTAGCAGTTTCGGGGCTTCTTTAGCAACTC 5 0.125 No Hit GTCTGCTGCTACTGTAACAACAATGGCAACAGGATTTTCTGGGTATACAA 5 0.125 No Hit TCTGGACTGCGGCCCATTCTTGGCCATCCATACCGAAAATGCTGTGAAGG 5 0.125 No Hit CTCTCTTGTCAAATAACAGCCCTGCAACATCGTCCAAGAATAGTGAAACT 5 0.125 No Hit CTTCTTTAGTCCTAGAGACCGTAAGTACCCAAATGGATCCCGACCCAATA 5 0.125 No Hit GGGAAACTCAAAAAAGGCCCTAACTGTCATCATAAACAAATTAGGGGACA 5 0.125 No Hit AATGGGCAATGCCCTAATGAATTGAGGGCTCCTGGGGGGTGTAATAACCC 5 0.125 No Hit TGTGTCTCGAGCGAGAGAAGGGCATTCGGTGGATGCCTTGGCACCAGGAG 5 0.125 No Hit GCTAATGTTAAAGTTTGGAGCTATGTTTATGACCTGTCAGGCTTTGGATT 5 0.125 No Hit ACCTAAGACTGCAAAACTTATAAATTCTCAGGGAAACTTAAGGCAAGTAA 5 0.125 No Hit GAATGGCTGCAAGTGTGGATCAAACTGCACCTGTGATCCATGCTCCTGCA 5 0.125 No Hit ATTCATAGCACAGGGCCAATTGGTTGTCTTGCTTACATCTTGACCGGCTT 5 0.125 No Hit GTCAAATTTGAATAGATCAAATCCAATTCCAGTTAATTCAGGTAAAGATT 5 0.125 No Hit TGAAGATAATGGATGTCCTGCATTTCTTGTTTGGAGTTTTTGGAAATGCC 5 0.125 No Hit CGTGATCGAGGTCCTTATCCAGCTGATCAAGTTGTTAAGGATCTTGATGG 5 0.125 No Hit AATGGGCCATGGACCTCATAGATGCTGCCGGTGGCAATCCCAATTTTAGA 5 0.125 No Hit GGAAAACTGTGCAAGAATCCGAGCTTTGCCACCGCGGATGATTTCTCGTA 5 0.125 No Hit AGAGACCCTCTCTCGGCCACCCACACAGCCGACAGACCCAGCTGTCTCTC 5 0.125 No Hit GCTCAGGCTAAGCAAGCAGCCGCCAACGCGCGCCGCAAGAGCAAGGCCAC 5 0.125 No Hit AAACAGTGTCTGGCACGGGACATAGTAAACATTGTAGCATGTGAGGGAGA 5 0.125 No Hit ATGTGAACCTTCGCTAAAAAGACAAACTTCTGAAGTCCCAAAGCAACAAT 5 0.125 No Hit AAATAATGAAAGCAATAGTAATAATAACAGTAACGCTGATTTAGTTGTTG 5 0.125 No Hit GCTCCTAACGTTGTTTACAAAGGGAAGCTGGAAAATCAGAGAAGGATTGC 5 0.125 No Hit GTACACGAGGCTGCAGAAGAGTTGCAAAATAAAGTGGATCGAAAATCATA 5 0.125 No Hit CAAGATTCTCAATCTCAAGCCGCCCCCTTTTATTTTCTCTGTTAACTAAA 5 0.125 No Hit CTCCGAGCCCAACTACCTCGAGAACTCGCTGAAGTACTCTGGACAGCAGA 5 0.125 No Hit TGAAAGAAGGGAATTGTGTAGGAATTGCCAGAATCAGAATCAGAATCAGA 5 0.125 No Hit GTTCGGGTGGCCTCGAGATATTTCAAGGGGCCTGAACTTCTTGTTGATTT 5 0.125 No Hit CATCGAGCAAAGTGGGTTCAAATTCCTGTGGTCCATGCGTTTTCCACGCT 5 0.125 No Hit CTGGTGAAGCACTTACACAAGGCATCATAGCAAGAAAATTGCACGAGAGG 5 0.125 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0125 0.0 0.0 0.0 0.0 62-63 0.025 0.0 0.0 0.0 0.0 64-65 0.075 0.0 0.0 0.0 0.0 66-67 0.0875 0.0 0.0 0.0 0.0 68-69 0.1 0.0 0.0 0.0 0.0 70-71 0.1 0.0 0.0 0.0 0.0 72-73 0.1 0.0 0.0 0.0 0.0 74-75 0.1 0.0 0.0 0.0 0.0 76-77 0.1 0.0 0.0 0.0 0.0 78-79 0.15 0.0 0.0 0.0 0.0 80-81 0.2375 0.0 0.0 0.0 0.0 82-83 0.325 0.0 0.0 0.0 0.0 84-85 0.425 0.0 0.0 0.0 0.0 86-87 0.6000000000000001 0.0 0.0 0.0 0.0 88-89 0.7749999999999999 0.0 0.0 0.0 0.0 90-91 1.0 0.0 0.0 0.0 0.0 92-93 1.1875 0.0 0.0 0.0 0.0 94-95 1.45 0.0 0.0 0.0 0.0 96-97 1.675 0.0 0.0 0.0 0.0 98-99 2.125 0.0 0.0 0.0 0.0 100-101 2.5625 0.0 0.0 0.0 0.0 102-103 2.85 0.0 0.0 0.0 0.0 104-105 3.075 0.0 0.0 0.0 0.0 106-107 3.4875 0.0 0.0 0.0 0.0 108-109 3.85 0.0 0.0 0.0 0.0 110-111 4.1625 0.0 0.0 0.0 0.0 112-113 4.5875 0.0 0.0 0.0 0.0 114-115 4.975 0.0 0.0 0.0 0.0 116-117 5.5375 0.0 0.0 0.0 0.0 118-119 5.949999999999999 0.0 0.0 0.0 0.0 120-121 6.300000000000001 0.0 0.0 0.0 0.0 122-123 6.5625 0.0 0.0 0.0 0.0 124-125 7.1875 0.0 0.0 0.0 0.0 126-127 7.75 0.0 0.0 0.0 0.0 128-129 8.287500000000001 0.0 0.0 0.0 0.0 130-131 8.85 0.0 0.0 0.0 0.0 132-133 9.3 0.0 0.0 0.0 0.0 134-135 9.9 0.0 0.0 0.0 0.0 136-137 10.4875 0.0 0.0 0.0 0.0 138-139 11.6625 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGGAGAT 10 0.006830828 145.0 1 CTAAAGG 10 0.006830828 145.0 6 GGGGGGG 35 0.0035366106 20.714287 140-144 >>END_MODULE Read 2200069 spots for SRR26075360.sra Written 2200069 spots for SRR26075360.sra Read 2200069 spots for SRR26075360.sra Written 2200069 spots for SRR26075360.sra Read 2200069 spots for SRR26075360.sra Written 2200069 spots for SRR26075360.sra Read 2200069 spots for SRR26075360.sra Written 2200069 spots for SRR26075360.sra Read 2200069 spots for SRR26075360.sra Written 2200069 spots for SRR26075360.sra Read 2200069 spots for SRR26075360.sra Written 2200069 spots for SRR26075360.sra Read 2200069 spots for SRR26075360.sra Written 2200069 spots for SRR26075360.sra Read 2200069 spots for SRR26075360.sra Written 2200069 spots for SRR26075360.sra Read 2200069 spots for SRR26075360.sra Written 2200069 spots for SRR26075360.sra Read 2200069 spots for SRR26075360.sra Written 2200069 spots for SRR26075360.sra Read 2200069 spots for SRR26075360.sra Written 2200069 spots for SRR26075360.sra Read 2200069 spots for SRR26075360.sra Written 2200069 spots for SRR26075360.sra Read 2200073 spots for SRR26075360.sra Written 2200073 spots for SRR26075360.sra Read 2200069 spots for SRR26075360.sra Written 2200069 spots for SRR26075360.sra Read 2200069 spots for SRR26075360.sra Written 2200069 spots for SRR26075360.sra Read 2200069 spots for SRR26075360.sra Written 2200069 spots for SRR26075360.sra Read 2200069 spots for SRR26075360.sra Written 2200069 spots for SRR26075360.sra Read 2200069 spots for SRR26075360.sra Written 2200069 spots for SRR26075360.sra Read 2200069 spots for SRR26075360.sra Written 2200069 spots for SRR26075360.sra Read 2200069 spots for SRR26075360.sra Written 2200069 spots for SRR26075360.sra SRR ids: ['SRR26075360.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_obmg2bi6 SRR26075360.sra spots: 44001384 blocks: [[1, 2200069], [2200070, 4400138], [4400139, 6600207], [6600208, 8800276], [8800277, 11000345], [11000346, 13200414], [13200415, 15400483], [15400484, 17600552], [17600553, 19800621], [19800622, 22000690], [22000691, 24200759], [24200760, 26400828], [26400829, 28600897], [28600898, 30800966], [30800967, 33001035], [33001036, 35201104], [35201105, 37401173], [37401174, 39601242], [39601243, 41801311], [41801312, 44001384]] SRR26075360 file size 16251849 SRR26075360 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o SRR26075360 SRR26075360_1.fastq SRR26075360_2.fastq Input file: SRR26075360_1.fastq Paired file: SRR26075360_2.fastq trimmed: SRR26075360-trimmed-pair1.fastq, SRR26075360-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Wed Feb 12 03:23:07 2025 >> started Wed Feb 12 03:23:56 2025 >> done (49.467s) 44001384 read pairs processed; of these: 258 ( 0.00%) short read pairs filtered out after trimming by size control 191766 ( 0.44%) empty read pairs filtered out after trimming by size control 43809360 (99.56%) read pairs available; of these: 7186417 (16.40%) trimmed read pairs available after processing 36622943 (83.60%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 18 13 0.00% 19 21 0.00% 20 19 0.00% 21 24 0.00% 22 29 0.00% 23 28 0.00% 24 46 0.00% 25 47 0.00% 26 37 0.00% 27 54 0.00% 28 59 0.00% 29 62 0.00% 30 56 0.00% 31 83 0.00% 32 92 0.00% 33 84 0.00% 34 80 0.00% 35 80 0.00% 36 131 0.00% 37 121 0.00% 38 172 0.00% 39 158 0.00% 40 150 0.00% 41 169 0.00% 42 184 0.00% 43 246 0.00% 44 200 0.00% 45 210 0.00% 46 241 0.00% 47 266 0.00% 48 332 0.00% 49 335 0.00% 50 430 0.00% 51 429 0.00% 52 569 0.00% 53 570 0.00% 54 614 0.00% 55 723 0.00% 56 726 0.00% 57 922 0.00% 58 1094 0.00% 59 1197 0.00% 60 1276 0.00% 61 1656 0.00% 62 1878 0.00% 63 2147 0.00% 64 2310 0.01% 65 2444 0.01% 66 2787 0.01% 67 2988 0.01% 68 3553 0.01% 69 3926 0.01% 70 4686 0.01% 71 5266 0.01% 72 6234 0.01% 73 7114 0.02% 74 7978 0.02% 75 8858 0.02% 76 9757 0.02% 77 10714 0.02% 78 11642 0.03% 79 13070 0.03% 80 14484 0.03% 81 16051 0.04% 82 17575 0.04% 83 19945 0.05% 84 21886 0.05% 85 24268 0.06% 86 25777 0.06% 87 28355 0.06% 88 29284 0.07% 89 32100 0.07% 90 33881 0.08% 91 36060 0.08% 92 38541 0.09% 93 41797 0.10% 94 44272 0.10% 95 47633 0.11% 96 51072 0.12% 97 53406 0.12% 98 54963 0.13% 99 57445 0.13% 100 60963 0.14% 101 61029 0.14% 102 64132 0.15% 103 68184 0.16% 104 70034 0.16% 105 73669 0.17% 106 77139 0.18% 107 81194 0.19% 108 82677 0.19% 109 85296 0.19% 110 85392 0.19% 111 89657 0.20% 112 90887 0.21% 113 91529 0.21% 114 96132 0.22% 115 100141 0.23% 116 102528 0.23% 117 106333 0.24% 118 112031 0.26% 119 111099 0.25% 120 113572 0.26% 121 116111 0.27% 122 115887 0.26% 123 119027 0.27% 124 122230 0.28% 125 124568 0.28% 126 128922 0.29% 127 132835 0.30% 128 135600 0.31% 129 139532 0.32% 130 141076 0.32% 131 142637 0.33% 132 144723 0.33% 133 147083 0.34% 134 146492 0.33% 135 148667 0.34% 136 153212 0.35% 137 154052 0.35% 138 159561 0.36% 139 164639 0.38% 140 165494 0.38% 141 167175 0.38% 142 168106 0.38% 143 168598 0.38% 144 171557 0.39% 145 173548 0.40% 146 174238 0.40% 147 177534 0.41% 148 180523 0.41% 149 182811 0.42% 150 186179 0.42% 151 36622943 83.60% 43809360 reads passed initial QC criterion=sequence-density sequence-density=0.30 sequence-density-rank=1 fanout-score=2.59 fanout-score-rank=31 prefix-density=0.39 prefix-fanout=2.0 sequence=CACTTGCAGCCATTCTC criterion=fanout-score sequence-density=0.01 sequence-density-rank=40 fanout-score=72.81 fanout-score-rank=1 prefix-density=0.10 prefix-fanout=7.6 sequence=GAAAACAAAAAACACTCCGCAATACATAATACACGGACCTCATTTCACCAGATTTTAGAGACATCACATAGTATTATACATCAAGTCGTCAACTCTCTGTTCTTAGCTTCTTACTCTGCACGCTTTTCTTCAACCATCTCCAAGGGGAAATGAGGAGACCCCCGAGGGCAAAAGATAACCCTATAGTTGGTCCCGCCAGGGCATGTAAATGTGCTTGTAGGGTCATCCT criterion=sequence-density sequence-density=0.34 sequence-density-rank=1 fanout-score=2.32 fanout-score-rank=33 prefix-density=0.35 prefix-fanout=2.3 sequence=ATGTACCCTGAC criterion=fanout-score sequence-density=0.01 sequence-density-rank=43 fanout-score=399.67 fanout-score-rank=1 prefix-density=0.18 prefix-fanout=12.6 sequence=AGAGAAAAGATAAGCTAGGCAAGATGGTTTTACTAGACAAGATGTGGGATGATGTTGTTGCTGGACCTCAGCCAGAACGTGGCCTTGG SRR26075360 testing PE reads STAR mapping to Ensembl genome Started job on | Feb 12 03:24:48 Started mapping on | Feb 12 03:24:48 Finished on | Feb 12 03:34:00 Mapping speed, Million of reads per hour | 285.71 Number of input reads | 43809360 Average input read length | 293 UNIQUE READS: Uniquely mapped reads number | 37878844 Uniquely mapped reads % | 86.46% Average mapped length | 292.12 Number of splices: Total | 35664262 Number of splices: Annotated (sjdb) | 34869852 Number of splices: GT/AG | 35013547 Number of splices: GC/AG | 507832 Number of splices: AT/AC | 36642 Number of splices: Non-canonical | 106241 Mismatch rate per base, % | 0.39% Deletion rate per base | 0.03% Deletion average length | 2.96 Insertion rate per base | 0.02% Insertion average length | 2.62 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 1149261 % of reads mapped to multiple loci | 2.62% Number of reads mapped to too many loci | 200369 % of reads mapped to too many loci | 0.46% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 10.07% % of reads unmapped: other | 0.39% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 4781255 4781255 4781255 N_multimapping 1149261 1149261 1149261 N_noFeature 792380 37437824 1058877 N_ambiguous 378718 2208 202520 UnstrandedReadsAssigned:36707746 PositiveStrandReadsAssigned:438812 NegativeStrandReadsAssigned:36617447 Dataset is classified negative stranded MeadianReadLen=151 20thPercentileLength=151 echo kmer=147 SRR26075360 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,400 [index] number of k-mers: 62,057,036 [index] number of equivalence classes: 130,681 [quant] running in paired-end mode [quant] will process pair 1: SRR26075360-trimmed-pair1.fastq SRR26075360-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 43,809,360 reads, 37,173,076 reads pseudoaligned [quant] estimated average fragment length: 214.648 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,119 rounds 52401 SRR26075360.ke.tsv 34699 SRR26075360.se.tsv 87100 total ==> SRR26075360.ke.tsv <== target_id length eff_length est_counts tpm Potri.005G200100.1.v4.1 2018 1804.35 3835 52.5724 Potri.005G024800.1.v4.1 1035 821.352 6109 183.973 Potri.004G059700.1.v4.1 961 747.358 0 0 Potri.007G009000.2.v4.1 1416 1202.35 0 0 Potri.003G141000.2.v4.1 2943 2729.35 2001.76 18.1412 Potri.016G087400.1.v4.1 270 91.6284 3474 937.807 Potri.015G069301.1.v4.1 564 352.76 0 0 Potri.010G195200.1.v4.1 1773 1559.35 2844 45.1127 Potri.012G127500.1.v4.1 977 763.358 41165 1333.87 ==> SRR26075360.se.tsv <== Potri.001G166300.v4.1 0 Potri.001G448400.v4.1 19 Potri.001G233950.v4.1 0 Potri.001G122700.v4.1 884 Potri.001G212900.v4.1 5 Potri.001G182400.v4.1 0 Potri.001G256600.v4.1 0 Potri.001G040500.v4.1 1 Potri.001G416900.v4.1 0 Potri.001G452600.v4.1 2887 SRR26075360 completed mapping pipeline successfully