Starting /dee2/code/volunteer_pipeline.sh SRR26075361 current disk space = 3052300828672 free memory = 1443686284 SRR26075361 SRAfilesize 6e86cc1b0f0e0207606709aa030ccdf9 SRR26075361.sra SRR26075361.sra file validated SRR26075361 is paired end SRR26075361 is conventional basespace SRR26075361 read1 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR26075361_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.39125 37.0 37.0 37.0 37.0 37.0 2 36.523 37.0 37.0 37.0 37.0 37.0 3 36.611 37.0 37.0 37.0 37.0 37.0 4 36.6595 37.0 37.0 37.0 37.0 37.0 5 36.695 37.0 37.0 37.0 37.0 37.0 6 36.631 37.0 37.0 37.0 37.0 37.0 7 36.648 37.0 37.0 37.0 37.0 37.0 8 36.664 37.0 37.0 37.0 37.0 37.0 9 36.6805 37.0 37.0 37.0 37.0 37.0 10-14 36.62395 37.0 37.0 37.0 37.0 37.0 15-19 36.59779999999999 37.0 37.0 37.0 37.0 37.0 20-24 36.557599999999994 37.0 37.0 37.0 37.0 37.0 25-29 36.4565 37.0 37.0 37.0 37.0 37.0 30-34 36.385299999999994 37.0 37.0 37.0 37.0 37.0 35-39 36.408500000000004 37.0 37.0 37.0 37.0 37.0 40-44 36.3063 37.0 37.0 37.0 37.0 37.0 45-49 36.164500000000004 37.0 37.0 37.0 37.0 37.0 50-54 36.1074 37.0 37.0 37.0 37.0 37.0 55-59 36.0221 37.0 37.0 37.0 37.0 37.0 60-64 35.948 37.0 37.0 37.0 37.0 37.0 65-69 35.8505 37.0 37.0 37.0 37.0 37.0 70-74 35.9524 37.0 37.0 37.0 37.0 37.0 75-79 35.965799999999994 37.0 37.0 37.0 37.0 37.0 80-84 35.989700000000006 37.0 37.0 37.0 37.0 37.0 85-89 35.8733 37.0 37.0 37.0 37.0 37.0 90-94 35.879599999999996 37.0 37.0 37.0 37.0 37.0 95-99 35.8242 37.0 37.0 37.0 37.0 37.0 100-104 35.8429 37.0 37.0 37.0 37.0 37.0 105-109 35.7554 37.0 37.0 37.0 37.0 37.0 110-114 35.6109 37.0 37.0 37.0 37.0 37.0 115-119 35.53000000000001 37.0 37.0 37.0 37.0 37.0 120-124 35.51220000000001 37.0 37.0 37.0 37.0 37.0 125-129 35.3611 37.0 37.0 37.0 37.0 37.0 130-134 35.0101 37.0 37.0 37.0 25.0 37.0 135-139 34.905 37.0 37.0 37.0 27.4 37.0 140-144 34.613600000000005 37.0 37.0 37.0 25.0 37.0 145-149 34.3895 37.0 37.0 37.0 25.0 37.0 150-151 34.416250000000005 37.0 37.0 37.0 25.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.0 1101 2 0.0 1101 3 0.0 1101 4 0.0 1101 5 0.0 1101 6 0.0 1101 7 0.0 1101 8 0.0 1101 9 0.0 1101 10-14 0.0 1101 15-19 0.0 1101 20-24 0.0 1101 25-29 0.0 1101 30-34 0.0 1101 35-39 0.0 1101 40-44 0.0 1101 45-49 0.0 1101 50-54 0.0 1101 55-59 0.0 1101 60-64 0.0 1101 65-69 0.0 1101 70-74 0.0 1101 75-79 0.0 1101 80-84 0.0 1101 85-89 0.0 1101 90-94 0.0 1101 95-99 0.0 1101 100-104 0.0 1101 105-109 0.0 1101 110-114 0.0 1101 115-119 0.0 1101 120-124 0.0 1101 125-129 0.0 1101 130-134 0.0 1101 135-139 0.0 1101 140-144 0.0 1101 145-149 0.0 1101 150-151 0.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 17 1.0 18 4.0 19 0.0 20 0.0 21 1.0 22 3.0 23 3.0 24 5.0 25 9.0 26 12.0 27 12.0 28 17.0 29 24.0 30 37.0 31 51.0 32 80.0 33 156.0 34 169.0 35 493.0 36 2741.0 37 182.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.27192762000503 13.772304599145514 8.293541090726313 35.66222669012314 2 18.525 14.95 36.0 30.525000000000002 3 15.875 15.575 31.225 37.325 4 22.975 23.575 25.3 28.15 5 22.925 29.925 26.05 21.099999999999998 6 23.0 30.975 25.15 20.875 7 16.325 26.924999999999997 40.275 16.475 8 18.475 25.874999999999996 32.15 23.5 9 19.325 24.575 34.25 21.85 10-14 19.717957693654046 28.949342401360205 28.40926138920838 22.923438515777367 15-19 20.330000000000002 27.855 27.905 23.91 20-24 19.72 27.900000000000002 27.834999999999997 24.545 25-29 19.885 27.905 28.455000000000002 23.755000000000003 30-34 19.02 27.77 28.99 24.22 35-39 20.24 27.49 27.400000000000002 24.87 40-44 19.375 28.68 28.035 23.91 45-49 19.689999999999998 27.689999999999998 27.834999999999997 24.785 50-54 21.305 27.18 26.93 24.585 55-59 20.325 27.944999999999997 27.134999999999998 24.595 60-64 21.19 26.87 27.560000000000002 24.38 65-69 20.21 27.61 27.88 24.3 70-74 21.14 27.71 27.27 23.880000000000003 75-79 21.475 27.625 28.075 22.825 80-84 21.975 26.015 28.13 23.880000000000003 85-89 20.294999999999998 27.29 28.375 24.04 90-94 21.834999999999997 27.76 26.724999999999998 23.68 95-99 21.375 28.025 26.14 24.46 100-104 21.63 27.46 26.669999999999998 24.240000000000002 105-109 22.105 27.415 26.919999999999998 23.56 110-114 22.165000000000003 26.200000000000003 27.685 23.95 115-119 22.470000000000002 26.97 26.715 23.845 120-124 22.31 28.185 25.025 24.48 125-129 22.485 27.655 26.400000000000002 23.46 130-134 22.275 28.389999999999997 25.61 23.724999999999998 135-139 22.54 26.889999999999997 26.105 24.465 140-144 22.78 27.21 25.34 24.67 145-149 23.3 26.72 25.52 24.46 150-151 24.175 26.474999999999998 25.374999999999996 23.974999999999998 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 0.0 1 0.5 2 0.5 3 0.5 4 1.0 5 0.5 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 1.0 19 1.0 20 3.0 21 3.0 22 0.0 23 1.0 24 2.0 25 4.0 26 4.0 27 4.5 28 6.0 29 10.0 30 15.5 31 16.5 32 22.5 33 37.0 34 42.0 35 52.5 36 70.5 37 93.5 38 126.0 39 148.5 40 169.5 41 182.0 42 235.0 43 279.0 44 258.0 45 260.5 46 257.5 47 250.0 48 259.5 49 235.0 50 185.0 51 138.5 52 125.0 53 108.5 54 80.0 55 65.0 56 53.0 57 48.0 58 33.0 59 19.0 60 12.5 61 8.5 62 8.5 63 7.0 64 5.0 65 2.5 66 3.5 67 6.5 68 10.0 69 10.0 70 7.5 71 5.5 72 3.0 73 1.5 74 0.5 75 0.0 76 0.0 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.525 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.015 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150-151 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 61.875 #Duplication Level Percentage of deduplicated Percentage of total 1 64.64646464646465 40.0 2 21.373737373737374 26.450000000000003 3 7.313131313131313 13.575000000000001 4 3.7575757575757573 9.3 5 1.7373737373737375 5.375 6 0.5252525252525253 1.95 7 0.2828282828282828 1.225 8 0.20202020202020202 1.0 9 0.0808080808080808 0.44999999999999996 >10 0.0808080808080808 0.675 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCTCCTAATCTCGTTT 15 0.375 TruSeq Adapter, Index 14 (97% over 39bp) TTCGATGATACGAGGTTGATACTGTAACAGCCCCCAAGCAACTTCTCATC 12 0.3 No Hit GTGGCATCCATCTTGTTGCAACAGCAAATCATCTGCTTCACACCAAGGGT 9 0.22499999999999998 No Hit CTTACGAAGACATTTTTTTCTGGATCAACTACAGAGATCCTGCACTCTTC 9 0.22499999999999998 No Hit TACGGTGGTTGCATGGAACACATTGAAGTGTTCATTTGTTACATTAACTC 8 0.2 No Hit GGCCTTGTTCTGAACTGACCAGCCCTCATCAAACTGGTTCTTGTTCCGAT 8 0.2 No Hit GGCATTTTCTTGGATTGTTATCTTAATTCTTCCCCTTTCATCAGCCTGTC 8 0.2 No Hit CCCAAGAGCATAGCTCCATCAATTTCCACCAATGTATAATGCTCTGAGTA 8 0.2 No Hit TGGTGATATTGATGCTGGTAATAATCTGGTGATTCTCCTTTTTCAATTGA 8 0.2 No Hit CTTTGCATTCACCACCGGGAAAACCTTCAGTCTTGCACACACTAGCACAG 7 0.17500000000000002 No Hit GCAGCTGAACCATAAGGGTTAAAGACTCGCTTGATAACACCATTGTCAGC 7 0.17500000000000002 No Hit CTGGCTCTGTCAGAAAAAGAAGGAGGGGTCGCAGCAACACTCGTCAACAA 7 0.17500000000000002 No Hit CTGTTGCAACCCTTGCAAGAACAGCATCTCTATTGACAGAGCCCTCTTTT 7 0.17500000000000002 No Hit CTCCAATCTCCTTTTCCCACTTTCTGCAATCCAAAAAGGAATCTCCTGTG 7 0.17500000000000002 No Hit ATCGATACTTGGGCGTATTGTACTTGTTCTTGTCCTGATTAATCAGTCGA 7 0.17500000000000002 No Hit GTTGTTGAAGGCCTTGCGAACACTTGGGTACTGGGGGGCACCATGGATAA 7 0.17500000000000002 No Hit CCTCAGTTGTCAAGCGCTTAGCTGCCGCTCTTGGATCCTTGATTGGTTTC 6 0.15 No Hit GTCCTGGTATCGTTGCCTATCCATTTCAGACCCGAATAAAATGTTCTCTT 6 0.15 No Hit GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCTCCTAATCTCGGTT 6 0.15 TruSeq Adapter, Index 14 (97% over 39bp) CTTTCCTTGTAACAGTCATTTGTTAGAGGCTTGACCCAGATAACAGTTTG 6 0.15 No Hit GTCCCTAATCACCGTCAATTTCTTGTCCTTCATGTCCATGGATATTGAAT 6 0.15 No Hit GGTGCCATCACTGGAGGTCCTTGATAATAACCTGTATATATGAGTCTCAA 6 0.15 No Hit GCTGTCCGGTCCATGGAATTTGCTGTCCAGATATGTTTTACAGCCAGTTC 6 0.15 No Hit CCGTAAGCTGTCAAGTGCATCCGAGCTTCCTTGGTAATGGTCCGGCCACT 6 0.15 No Hit ATTGGGACCTCAGAGCCCTAATTGGTATCCAAATCATTAATCTACATTGA 6 0.15 No Hit ACCTGCTTTTGCTTTTCCTCAATCTTATAAACTATGAAGCGGTAAGTTCT 6 0.15 No Hit CCCCGGCAAACAGCAAAGAATTAAACATAACGTAACGAATTACGCAGAAT 6 0.15 No Hit GAGGTCAGCAGCAATCTCATCATTAGTAACAGTTTCATCATGTTGCGTAG 6 0.15 No Hit TTTCTCTTTGTCTATAACAAGACCTCTAATTACCAAGTCTGGATCAAATG 6 0.15 No Hit GTCGCAATTCCGCAGGGAGGCGTGGGAAAGGATGGAATTCGAGGGGAGGA 5 0.125 No Hit AGCCTTCCCAGCAAACCCATCAACGCAAGTATTCTCATCAGTCAATGCAG 5 0.125 No Hit GCCCAATATAACAAATCCCATCACAGCTGCAGCCACAGAGACTGACCAAA 5 0.125 No Hit AGCAGACTTGTAAGCCAACAAAGTGCTCTCAGCAGCTTCCTTCCTCTCAG 5 0.125 No Hit GGTGCTTCCTCTTCAACCTCCTCATACGCTTCTTCTTCCACTTGGCTCTC 5 0.125 No Hit TGGATGGCTTTCTCTTTCTAATAAAATCCGTGCTGGTGATAACTTCTCCC 5 0.125 No Hit GGCTTGTCTGTGGGTCTCTTGGGCTCACTGATCTGGTCAAGAGCATCAAG 5 0.125 No Hit GAGGGTTCATCGCAACCTGGTTCTTAGCTGCATCACCTATCAAACGTTCA 5 0.125 No Hit TAAAAGAAGATAATAATACTAACAAGTACACCCTTGAAAAACCATTCTAT 5 0.125 No Hit CCACTGATGAGTTCATTTCCATAAACCAAGCTCAAATCTTGATAGTTTAG 5 0.125 No Hit CTCCGTACTTTTCAAAGACTGACAAAACACGGTAGCGAAATTGGGTTTGT 5 0.125 No Hit GAGCTCATGTCAGGGTACATCTTGCATCCTCCACAGCCGCTGCCGCACTT 5 0.125 No Hit CACATTCTGGGCTCGAAATGCCCTCATGGCTTGTTGCACCAAGAAAGCAC 5 0.125 No Hit GGGGTAGCCGCAGCCGGGAACGTAAATATCTGGGGCTGGTTAGACGGTCC 5 0.125 No Hit GTGTGAACACGGTGAGCTAATACAGGAGAAAGCTTTGGAACATCACGCAT 5 0.125 No Hit CCTGTGAATCCTAGCAGCAAATGTGTTTGGATCATCTAGGCTGAAGCCAG 5 0.125 No Hit AGCAGCGAAACAAAACAAAGGCGAGAAAACAGTTGGAAGACTCTTGGGTT 5 0.125 No Hit CAGAACACTAGAAAATTATTGCTACAGGATGGGCTAAAAGCAATACATCA 5 0.125 No Hit CTCCAACATGTCCAGAAGCAGCATAGATACAGGTATAAAAGTACCAGTTG 5 0.125 No Hit GCTATCATAATCGGCGCCGAGATAGTGTAGCCCAATATATTAGTAGACAA 5 0.125 No Hit GCCAGTTTCTTCCTTGGGACAGATGAAAATCCTATGGACCGCTGATAGGA 5 0.125 No Hit CCACTCTAACACCAGCATTGAACCATGACATTTCACCATCCTCACCGTCC 5 0.125 No Hit CTTCATCGTCACCTTCCTCCTCGTCGCTAGAAGAGCTGGAAGAACTGCTG 5 0.125 No Hit CTTTCAAGATTTCTTGATTTCCTTGTTTTAATCTGTTTTGAATTCGAGGG 5 0.125 No Hit CCGAGATTTAATATGGATATGTACTCCATCGCTCTCCCATTCACAGTTTT 5 0.125 No Hit GTTCCGTAACCCACGGCAGCAACACCATGGTCCAGACTTGTCCCACATTC 5 0.125 No Hit GCTCATCATCGAAGGAAACCTCCTCTTTAAAGACCCCGGCTTTCCCTCCG 5 0.125 No Hit AGACCAAACAAGTTTTATTGCTTCTGGGGTGCCAATCCCGGCACCTCTCG 5 0.125 No Hit GTCCAATCAAAAACATAGTCAAACTGGAAACCTTCACGAATAAAAAGATC 5 0.125 No Hit CACCTTGATAGTTTATCCAGCCCCCCCTTTCCATGCCCTTCAATGTTCTC 5 0.125 No Hit GGGGTTTCGTAGAAGTGGGGTGGTCCTAATGGACTTGGGTTGACCAACAG 5 0.125 No Hit CTTCAGTAGCTTCTGACTTTTGGGGGCTCTGCTTAGCTGCACGAGAAAGA 5 0.125 No Hit CTTCCCTTGGCCTCGGAGTACGCCAATTCATACCACAAAGAACTGCTAGA 5 0.125 No Hit CAGCAAATGAGGACAGCAGCAAAGGCATAGAGGGCCATGAAGGCAGAGTT 5 0.125 No Hit GTCTCTTATACTTGTAAAACTGAGCAGCCCACATTTGATGCTGCCCAACC 5 0.125 No Hit ATAAGCAATTTTAACTCTTCAATCATAATGCTTGTTATCTTCAAAATATT 5 0.125 No Hit CTCAGAATGAAGAGGAATTACACGCCTCAGGCTCATGTCCATATTTCCGT 5 0.125 No Hit GACCGAAGCCTCTAAATTCCCATTTTTCTCTTCTTCTTTAGTCCCATTTT 5 0.125 No Hit CATTTTTATCCACATCAATGGCATCAAATTGATCCTTAAGATCAGCTAGT 5 0.125 No Hit CACTCACGTTGTTTTCGGCAAGAGCTTTAGCACATGCAATCAACAGCTGC 5 0.125 No Hit CACCCATGTCAGCACCGTCGCCCTGATACATCTTTGCAATTATAGGATTG 5 0.125 No Hit AGAAAGACCTTCATGACCAATCTTTTGCAGAAACCACTTGCTGCGTGAAT 5 0.125 No Hit GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCTCCTAATCGCGGTT 5 0.125 TruSeq Adapter, Index 14 (97% over 39bp) >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0 0.0 0.0 0.0 0.0 64-65 0.0375 0.0 0.0 0.0 0.0 66-67 0.0875 0.0 0.0 0.0 0.0 68-69 0.1125 0.0 0.0 0.0 0.0 70-71 0.15 0.0 0.0 0.0 0.0 72-73 0.25 0.0 0.0 0.0 0.0 74-75 0.3 0.0 0.0 0.0 0.0 76-77 0.36250000000000004 0.0 0.0 0.0 0.0 78-79 0.5874999999999999 0.0 0.0 0.0 0.0 80-81 0.75 0.0 0.0 0.0 0.0 82-83 1.0125 0.0 0.0 0.0 0.0 84-85 1.2875 0.0 0.0 0.0 0.0 86-87 1.6125 0.0 0.0 0.0 0.0 88-89 2.1375 0.0 0.0 0.0 0.0 90-91 2.4875 0.0 0.0 0.0 0.0 92-93 2.75 0.0 0.0 0.0 0.0 94-95 3.0 0.0 0.0 0.0 0.0 96-97 3.45 0.0 0.0 0.0 0.0 98-99 4.0375 0.0 0.0 0.0 0.0 100-101 4.65 0.0 0.0 0.0 0.0 102-103 4.9375 0.0 0.0 0.0 0.0 104-105 5.325 0.0 0.0 0.0 0.0 106-107 6.025 0.0 0.0 0.0 0.0 108-109 6.35 0.0 0.0 0.0 0.0 110-111 6.75 0.0 0.0 0.0 0.0 112-113 7.35 0.0 0.0 0.0 0.0 114-115 7.9 0.0 0.0 0.0 0.0 116-117 8.6125 0.0 0.0 0.0 0.0 118-119 9.425 0.0 0.0 0.0 0.0 120-121 10.024999999999999 0.0 0.0 0.0 0.0 122-123 10.925 0.0 0.0 0.0 0.0 124-125 11.825 0.0 0.0 0.0 0.0 126-127 12.7625 0.0 0.0 0.0 0.0 128-129 13.662500000000001 0.0 0.0 0.0 0.0 130-131 14.412500000000001 0.0 0.0 0.0 0.0 132-133 15.75 0.0 0.0 0.0 0.0 134-135 17.275 0.0 0.0 0.0 0.0 136-137 18.575 0.0 0.0 0.0 0.0 138-139 19.4875 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTTAAGC 10 0.006830828 145.0 6 AAGCATC 10 0.006830828 145.0 8 >>END_MODULE SRR26075361 read2 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR26075361_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.247 37.0 37.0 37.0 37.0 37.0 2 36.326 37.0 37.0 37.0 37.0 37.0 3 36.2805 37.0 37.0 37.0 37.0 37.0 4 36.2595 37.0 37.0 37.0 37.0 37.0 5 36.391 37.0 37.0 37.0 37.0 37.0 6 36.223 37.0 37.0 37.0 37.0 37.0 7 36.2165 37.0 37.0 37.0 37.0 37.0 8 36.274 37.0 37.0 37.0 37.0 37.0 9 36.242 37.0 37.0 37.0 37.0 37.0 10-14 36.1769 37.0 37.0 37.0 37.0 37.0 15-19 36.090599999999995 37.0 37.0 37.0 37.0 37.0 20-24 36.022 37.0 37.0 37.0 37.0 37.0 25-29 35.8249 37.0 37.0 37.0 37.0 37.0 30-34 35.6564 37.0 37.0 37.0 37.0 37.0 35-39 35.621900000000004 37.0 37.0 37.0 37.0 37.0 40-44 35.5897 37.0 37.0 37.0 37.0 37.0 45-49 35.528600000000004 37.0 37.0 37.0 37.0 37.0 50-54 35.3986 37.0 37.0 37.0 37.0 37.0 55-59 35.4037 37.0 37.0 37.0 37.0 37.0 60-64 35.5293 37.0 37.0 37.0 37.0 37.0 65-69 35.408500000000004 37.0 37.0 37.0 37.0 37.0 70-74 35.3604 37.0 37.0 37.0 37.0 37.0 75-79 35.243700000000004 37.0 37.0 37.0 37.0 37.0 80-84 35.268299999999996 37.0 37.0 37.0 37.0 37.0 85-89 35.2846 37.0 37.0 37.0 37.0 37.0 90-94 35.169200000000004 37.0 37.0 37.0 34.6 37.0 95-99 35.275800000000004 37.0 37.0 37.0 37.0 37.0 100-104 35.1772 37.0 37.0 37.0 34.6 37.0 105-109 35.124900000000004 37.0 37.0 37.0 29.8 37.0 110-114 35.110600000000005 37.0 37.0 37.0 32.2 37.0 115-119 35.094100000000005 37.0 37.0 37.0 27.4 37.0 120-124 34.945 37.0 37.0 37.0 25.0 37.0 125-129 34.9681 37.0 37.0 37.0 27.4 37.0 130-134 34.89 37.0 37.0 37.0 25.0 37.0 135-139 34.7371 37.0 37.0 37.0 25.0 37.0 140-144 34.680099999999996 37.0 37.0 37.0 25.0 37.0 145-149 34.638999999999996 37.0 37.0 37.0 25.0 37.0 150-151 34.26575 37.0 37.0 37.0 25.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.0 1101 2 0.0 1101 3 0.0 1101 4 0.0 1101 5 0.0 1101 6 0.0 1101 7 0.0 1101 8 0.0 1101 9 0.0 1101 10-14 0.0 1101 15-19 0.0 1101 20-24 0.0 1101 25-29 0.0 1101 30-34 0.0 1101 35-39 0.0 1101 40-44 0.0 1101 45-49 0.0 1101 50-54 0.0 1101 55-59 0.0 1101 60-64 0.0 1101 65-69 0.0 1101 70-74 0.0 1101 75-79 0.0 1101 80-84 0.0 1101 85-89 0.0 1101 90-94 0.0 1101 95-99 0.0 1101 100-104 0.0 1101 105-109 0.0 1101 110-114 0.0 1101 115-119 0.0 1101 120-124 0.0 1101 125-129 0.0 1101 130-134 0.0 1101 135-139 0.0 1101 140-144 0.0 1101 145-149 0.0 1101 150-151 0.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 12 2.0 13 4.0 14 6.0 15 16.0 16 13.0 17 7.0 18 5.0 19 7.0 20 7.0 21 10.0 22 11.0 23 10.0 24 22.0 25 21.0 26 14.0 27 23.0 28 23.0 29 17.0 30 27.0 31 40.0 32 59.0 33 87.0 34 206.0 35 688.0 36 2479.0 37 196.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 43.125 22.2 10.299999999999999 24.375 2 29.7 26.825 27.35 16.125 3 24.125 28.775000000000002 29.775000000000002 17.325 4 25.224999999999998 34.125 22.575 18.075 5 25.474999999999998 38.224999999999994 21.325 14.975 6 24.825 39.074999999999996 21.725 14.374999999999998 7 23.549999999999997 22.25 36.175000000000004 18.025 8 24.425 25.75 25.55 24.275 9 25.224999999999998 24.95 26.724999999999998 23.1 10-14 25.330000000000002 28.59 25.169999999999998 20.91 15-19 26.125 27.6 25.724999999999998 20.549999999999997 20-24 25.535000000000004 27.944999999999997 26.534999999999997 19.985 25-29 26.31 27.21 26.565 19.915 30-34 25.165 28.54 25.72 20.575 35-39 25.41 27.935 25.990000000000002 20.665 40-44 25.645 27.925 25.64 20.79 45-49 25.635 28.194999999999997 25.46 20.71 50-54 24.0 28.08 27.63 20.29 55-59 26.369999999999997 26.979999999999997 27.200000000000003 19.45 60-64 25.575 27.165 27.07 20.19 65-69 26.009999999999998 27.32 26.169999999999998 20.5 70-74 24.43 28.255000000000003 27.435 19.88 75-79 23.79 29.080000000000002 26.279999999999998 20.849999999999998 80-84 24.59 27.900000000000002 27.345000000000002 20.165 85-89 26.215 29.525000000000002 25.15 19.11 90-94 24.905 28.42 26.455000000000002 20.22 95-99 25.369999999999997 29.630000000000003 25.480000000000004 19.52 100-104 25.775 27.68 25.36 21.185000000000002 105-109 26.400000000000002 28.275 26.21 19.115 110-114 25.97 27.700000000000003 26.27 20.06 115-119 25.91 27.925 26.619999999999997 19.545 120-124 26.009999999999998 27.800000000000004 26.529999999999998 19.66 125-129 27.439999999999998 28.12 25.745 18.695 130-134 28.660000000000004 26.895000000000003 25.985000000000003 18.459999999999997 135-139 27.21 28.835 25.195 18.759999999999998 140-144 28.58 28.04 25.240000000000002 18.14 145-149 28.189999999999998 27.74 25.34 18.73 150-151 29.7375 27.6625 24.6625 17.9375 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.5 4 0.5 5 0.0 6 0.0 7 0.0 8 1.0 9 1.0 10 0.5 11 1.5 12 1.0 13 0.5 14 1.5 15 2.0 16 1.0 17 0.0 18 0.0 19 1.0 20 2.0 21 1.5 22 1.0 23 0.5 24 0.0 25 0.5 26 2.0 27 3.5 28 4.5 29 5.0 30 7.5 31 11.0 32 13.5 33 20.5 34 33.0 35 50.0 36 63.0 37 76.0 38 109.5 39 147.5 40 190.5 41 221.0 42 249.0 43 277.0 44 290.5 45 281.0 46 242.0 47 245.5 48 255.5 49 218.5 50 175.5 51 151.5 52 122.5 53 91.5 54 83.5 55 62.5 56 39.5 57 38.5 58 34.5 59 19.5 60 10.0 61 9.0 62 10.0 63 9.5 64 6.5 65 8.0 66 7.0 67 4.5 68 4.5 69 2.5 70 0.0 71 1.0 72 2.0 73 1.0 74 2.0 75 2.5 76 0.5 77 1.5 78 2.0 79 1.5 80 1.5 81 0.5 82 1.5 83 4.0 84 5.0 85 5.0 86 4.5 87 5.5 88 5.0 89 2.0 90 2.0 91 1.5 92 0.5 93 1.0 94 0.5 95 0.0 96 1.0 97 1.5 98 1.0 99 1.5 100 8.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150-151 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 62.7 #Duplication Level Percentage of deduplicated Percentage of total 1 66.46730462519936 41.675000000000004 2 20.25518341307815 25.4 3 6.977671451355662 13.125 4 3.508771929824561 8.799999999999999 5 1.674641148325359 5.25 6 0.4784688995215311 1.7999999999999998 7 0.23923444976076555 1.05 8 0.23923444976076555 1.2 9 0.03987240829346093 0.22499999999999998 >10 0.11961722488038277 1.4749999999999999 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG 25 0.625 No Hit GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGT 23 0.575 No Hit GAAAATACAGTTCCTCTCCTTGCCTCTGGGGTTTCAGTTATTGTAGGGAT 11 0.27499999999999997 No Hit GGAAGGAGGAGTATGATAGGGCACGAGCTCGCATCTTCAGTAGCCCTAGC 9 0.22499999999999998 No Hit GTGGAACATCTTCAGGTTCATCTTTGATTCAGAACTCGGGTTCTGAAGAG 8 0.2 No Hit CCTCAAGAGGAGGTGATTGGTACTGAATTTGAAGAGAAACTTCAGGTTTC 8 0.2 No Hit GGACTCCATATAGATCAAGAAGACTGCCCATATTTGAAGAGATCTCGCCA 8 0.2 No Hit AACCAAAAGAAGCTGTCGCAGGCGGTTGGCCGGACACAATGAGAGGCGAA 8 0.2 No Hit GTCAGATTGCCGAGAAACCAGAAGGAGCGAATTAGTGTGGAGCAGCATTG 8 0.2 No Hit GAGATGTTCTTGTGCAGAAATTATGTGTGCCGGTAAACCTTTTGATAGCT 8 0.2 No Hit CAGAAATCTAAGGCCTACTCCAAGAGGTTTCAAGTCAAGTTTAAGAGAAG 7 0.17500000000000002 No Hit CCTCAACAGTACCCCAACCAGTACCCCAACCATGCCTCCAAGCCCATCCC 7 0.17500000000000002 No Hit CTAAAGGCTCTGCCTACCAAACTAGGCAGCGGCAAGGCTCGAACACCACG 7 0.17500000000000002 No Hit CTCTGTTATCTTTATAGCTATCTTCTTGTAGTTTTTTGTCGGCTCCATAA 7 0.17500000000000002 No Hit CATCTAAAATCCTGAAGAATCTTTGGTAGAGAGATGAGTGACCCCAAGTA 7 0.17500000000000002 No Hit GATAATAATAATGAGGAGGAGGATGCTGGTGCTGCTGTTTCTGGTTATAT 7 0.17500000000000002 No Hit GTTTGAGACAACTAGGTACTACTGCACTGTCATTGATGCCCCTGGTCATC 6 0.15 No Hit AATCAGCTTCGAGTATTTGTATCTTGTTTTGTTGCTGGGTTGCTAGTTTT 6 0.15 No Hit TAACCTCTAAAATTCTGAAGAATCTTTGGTAGAGAGATGAGTGACCCCAA 6 0.15 No Hit GATTTTCAAGCTAACTGTTAATAGTTGAAGAGAAGCTGAAGAGGAAAAGA 6 0.15 No Hit GCAAAAGACAATGCATTTGAGGTGATCTTCATCTCAAGTGACAGCGATCA 6 0.15 No Hit GGAGTTGCAAGTTTTGGGGGATATCTGCTCTCTTCTGATATTATTTCAAT 6 0.15 No Hit GGAATTCATCCCGCGGCATATTTTGCAGTCGTACTTTAAATCTTAGGTCA 6 0.15 No Hit AAGAAGTATGAACCGGTGATTGAGCTGCCCTAGAAGAATATCCAGCAGGA 6 0.15 No Hit AGTGATTTAGCCTTCGCATGGTGGTTTGGAGCCGCCTGACTTCCATAACC 6 0.15 No Hit GCACAATCATGGCCAACGCAGCATCTGGTATGGCTGTGCATGATGACTGC 6 0.15 No Hit CGCAAGAATGGAACCTGCCCTTGGTTGAGACCTGATGGCAAAACACAAGT 6 0.15 No Hit GTCAAAGCCCACATTGAGAAATGTGAGTTTAAAGATCATGCCTGGTGAAA 6 0.15 No Hit GATCATGACAAGCCTGTTCAACAGGTGATTATCGAGATGACTGATGGAGG 5 0.125 No Hit CCACCTTCCTGCTTTCTCTCACTGTAAAACCAAAAAGCTAAAAAAAACCT 5 0.125 No Hit GGAATTCTCAGTCTCCCACCTAGCAGCAACCCGACAGGTGCTTTGACAGA 5 0.125 No Hit GGTTTTCCAATCAAAGAAAGAAGGGAGCACAAAGCTTGGTTGCCTTGCCG 5 0.125 No Hit AGGGGAGAATGTTGTGTACCGGTTTGGTTCTGTTGGTCAGGACACACAGT 5 0.125 No Hit ATCACCACCACCACCCTCACCCCCACCACCATCACAAACAACCACCAGTG 5 0.125 No Hit GTTCCCTTTTTAACACTTTCTTGCTAAAACATCCATAATGGCATCAAAAA 5 0.125 No Hit GGGACTACTTATGCTGGATTAAGAGCAAGAACAACTGGGGCTCCACAGAA 5 0.125 No Hit AGATTTGTGTTGATATAGAAAACAATGGCACTACATGGAAAGATTGAGAC 5 0.125 No Hit GAGGAGTTGCATCTGAGATTCCTATTGACATATCTGTCCTGAGTAACATG 5 0.125 No Hit GGAGATAAAGGTGTTTGATGCTCAACTTGCCTTATGCTTTTTGCACACCT 5 0.125 No Hit ACCTGATGTTTGACTTCCAAGGAAGTTTGATTGATGGGACCAGCGGGTGG 5 0.125 No Hit AGCAAATGCGTGCCGTGCTCTCATGTGTATTGCAAAGTGTGTATTTCGCG 5 0.125 No Hit ATGCTCTTGATGATCGGGTTAAGAAGAAAGCTGATCGTTACTGTAAATGA 5 0.125 No Hit GAGTTATTGGATTAGATGGGACTCGTCTCCTAACCGGCAGCTCATTCATG 5 0.125 No Hit CGCCAATCAACTTACAGTGGACCATGAGCCACACACTGAAAGAAAAAGGA 5 0.125 No Hit TGTTAGACAGGTTCTTGTTAGACAGAGAGATGGAGAAGAAATGCTATGGT 5 0.125 No Hit GTTTAAGCTACTCAAGCTCAAGCTCTTCTTGTCTTCTCAGCTCTGCCTCT 5 0.125 No Hit CAAGATTACTATCACTAACGATAAGGGAAGGCTGTCCAAGGAAGAGATTG 5 0.125 No Hit GAGAAAGGGGATGTTAGATTCCATACCAGAATATGAGGCAACTGAAAGCC 5 0.125 No Hit GGGGATGTCAAGGTGGCTTTACAAAGGATCAACAAGATTTTGGAGAGCAG 5 0.125 No Hit CTTGTGTCGGGGTTATCCTACGGAATTTGCATCCTATTTCCATTACTGTC 5 0.125 No Hit TTCAGCTTTTATATCCCCTATTCTCTTCGTGAAGATGTCTTGCTGTGGAG 5 0.125 No Hit ACACCCTGATATGCATTCATATAGAAAGAAGACCTTACTAAACTATCAAG 5 0.125 No Hit CTTCTCTTATTGTCTACCCAGTTTCAAGAGCTACTACTTCTCAGGTTCTC 5 0.125 No Hit CTTCTTGATACCTTGATACCTTGATTGGCAATACCTGTTTCAATCCTTCT 5 0.125 No Hit GTGACAGGAGAGTATGGCTGGACAGCTAACATGGAGAGGATCATGAAGGC 5 0.125 No Hit GGAGATTCTCAACATCGTGAAGGAAAAATTTGACTTCAGGCCTGGAATGA 5 0.125 No Hit TGGTAAAAGTGATAGAAACAGCCCCCACCACAGGAAGCATGCGACGTCCT 5 0.125 No Hit GGGATGAGCTGGTGGAGCAATAAAACAGGTACAAATCGAGCCAAGGCTCG 5 0.125 No Hit TGGTGGTGATGATTTGTTTTCTGGTGATGTCGGGAAGGATCAAGTTGGGT 5 0.125 No Hit AAATCAGCCAGTGAGTGTTGCAATTGAAGGTGGTGGCAGAAATTTCCAGT 5 0.125 No Hit CAGCAGGCCACAGACATCAGATCAGAAACCTAAGGCCTGGCGCCAGGGTT 5 0.125 No Hit GGGAGTGGATCTTTGGTCGTTAATGATTCCAGTGAGTATGATGTTGAAGG 5 0.125 No Hit GGAATAGTTATAGAACGGATTTGGAGGGGCCAAATGGGGTTATCAACTGA 5 0.125 No Hit CTTAAAACGAATATAACCACTCTGGGCCCATTAGTTCTTCCAGTCTCCGA 5 0.125 No Hit AGGAAACCTATCAAACGCCATGAAAGGTAATAATAGCACAAAGCACCCTC 5 0.125 No Hit GAATGGAAGTTCATGAATTTCCTTGAACTTTGGACTGGAGCTATCTGCAC 5 0.125 No Hit ACGAGATTCCCACTGTCCCTGTCTACTATCCAGCGAAACCACAGCCAAGG 5 0.125 No Hit CAGGGAGAGGAAAAAAAGAAGAAGGTGTGTGGGAATCCTCTCTGGAACAG 5 0.125 No Hit CTTCTAAAGGTTTTACTTTTGGTGAGGTTGGCTGCATACGGACAAGAAAT 5 0.125 No Hit AGCTTATGAACAGGTTTCACCAGCAGTCCCTTCTTGGCTAAACAAAGGAG 5 0.125 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0 0.0 0.0 0.0 0.0 64-65 0.025 0.0 0.0 0.0 0.0 66-67 0.0625 0.0 0.0 0.0 0.0 68-69 0.0875 0.0 0.0 0.0 0.0 70-71 0.1125 0.0 0.0 0.0 0.0 72-73 0.2 0.0 0.0 0.0 0.0 74-75 0.25 0.0 0.0 0.0 0.0 76-77 0.3125 0.0 0.0 0.0 0.0 78-79 0.5375000000000001 0.0 0.0 0.0 0.0 80-81 0.7 0.0 0.0 0.0 0.0 82-83 0.9624999999999999 0.0 0.0 0.0 0.0 84-85 1.2375 0.0 0.0 0.0 0.0 86-87 1.5625 0.0 0.0 0.0 0.0 88-89 2.1 0.0 0.0 0.0 0.0 90-91 2.4625000000000004 0.0 0.0 0.0 0.0 92-93 2.725 0.0 0.0 0.0 0.0 94-95 3.025 0.0 0.0 0.0 0.0 96-97 3.5 0.0 0.0 0.0 0.0 98-99 4.0875 0.0 0.0 0.0 0.0 100-101 4.675 0.0 0.0 0.0 0.0 102-103 4.9875 0.0 0.0 0.0 0.0 104-105 5.4 0.0 0.0 0.0 0.0 106-107 6.125 0.0 0.0 0.0 0.0 108-109 6.4625 0.0 0.0 0.0 0.0 110-111 6.9 0.0 0.0 0.0 0.0 112-113 7.525 0.0 0.0 0.0 0.0 114-115 8.05 0.0 0.0 0.0 0.0 116-117 8.8125 0.0 0.0 0.0 0.0 118-119 9.625 0.0 0.0 0.0 0.0 120-121 10.25 0.0 0.0 0.0 0.0 122-123 11.175 0.0 0.0 0.0 0.0 124-125 12.025 0.0 0.0 0.0 0.0 126-127 13.075 0.0 0.0 0.0 0.0 128-129 14.0625 0.0 0.0 0.0 0.0 130-131 14.825 0.0 0.0 0.0 0.0 132-133 16.1875 0.0 0.0 0.0 0.0 134-135 17.85 0.0 0.0 0.0 0.0 136-137 19.112499999999997 0.0 0.0 0.0 0.0 138-139 19.9375 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCAAACA 10 0.006830828 145.0 4 CCATCGC 10 0.006830828 145.0 4 CAAACAC 10 0.006830828 145.0 5 AAGAGCG 95 0.007278115 10.684211 140-144 >>END_MODULE Read 3227549 spots for SRR26075361.sra Written 3227549 spots for SRR26075361.sra Read 3227549 spots for SRR26075361.sra Written 3227549 spots for SRR26075361.sra Read 3227549 spots for SRR26075361.sra Written 3227549 spots for SRR26075361.sra Read 3227549 spots for SRR26075361.sra Written 3227549 spots for SRR26075361.sra Read 3227560 spots for SRR26075361.sra Written 3227560 spots for SRR26075361.sra Read 3227549 spots for SRR26075361.sra Written 3227549 spots for SRR26075361.sra Read 3227549 spots for SRR26075361.sra Written 3227549 spots for SRR26075361.sra Read 3227549 spots for SRR26075361.sra Written 3227549 spots for SRR26075361.sra Read 3227549 spots for SRR26075361.sra Written 3227549 spots for SRR26075361.sra Read 3227549 spots for SRR26075361.sra Written 3227549 spots for SRR26075361.sra Read 3227549 spots for SRR26075361.sra Written 3227549 spots for SRR26075361.sra Read 3227549 spots for SRR26075361.sra Written 3227549 spots for SRR26075361.sra Read 3227549 spots for SRR26075361.sra Written 3227549 spots for SRR26075361.sra Read 3227549 spots for SRR26075361.sra Written 3227549 spots for SRR26075361.sra Read 3227549 spots for SRR26075361.sra Written 3227549 spots for SRR26075361.sra Read 3227549 spots for SRR26075361.sra Written 3227549 spots for SRR26075361.sra Read 3227549 spots for SRR26075361.sra Written 3227549 spots for SRR26075361.sra Read 3227549 spots for SRR26075361.sra Written 3227549 spots for SRR26075361.sra Read 3227549 spots for SRR26075361.sra Written 3227549 spots for SRR26075361.sra Read 3227549 spots for SRR26075361.sra Written 3227549 spots for SRR26075361.sra SRR ids: ['SRR26075361.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_v9auxok0 SRR26075361.sra spots: 64550991 blocks: [[1, 3227549], [3227550, 6455098], [6455099, 9682647], [9682648, 12910196], [12910197, 16137745], [16137746, 19365294], [19365295, 22592843], [22592844, 25820392], [25820393, 29047941], [29047942, 32275490], [32275491, 35503039], [35503040, 38730588], [38730589, 41958137], [41958138, 45185686], [45185687, 48413235], [48413236, 51640784], [51640785, 54868333], [54868334, 58095882], [58095883, 61323431], [61323432, 64550991]] SRR26075361 file size 23846872 SRR26075361 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o SRR26075361 SRR26075361_1.fastq SRR26075361_2.fastq Input file: SRR26075361_1.fastq Paired file: SRR26075361_2.fastq trimmed: SRR26075361-trimmed-pair1.fastq, SRR26075361-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Tue Feb 11 22:37:54 2025 >> started Tue Feb 11 22:39:11 2025 >> done (76.973s) 64550991 read pairs processed; of these: 467 ( 0.00%) short read pairs filtered out after trimming by size control 649731 ( 1.01%) empty read pairs filtered out after trimming by size control 63900793 (98.99%) read pairs available; of these: 15898109 (24.88%) trimmed read pairs available after processing 48002684 (75.12%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 18 46 0.00% 19 47 0.00% 20 45 0.00% 21 48 0.00% 22 64 0.00% 23 79 0.00% 24 122 0.00% 25 104 0.00% 26 86 0.00% 27 104 0.00% 28 143 0.00% 29 109 0.00% 30 117 0.00% 31 167 0.00% 32 184 0.00% 33 164 0.00% 34 221 0.00% 35 213 0.00% 36 250 0.00% 37 235 0.00% 38 256 0.00% 39 394 0.00% 40 341 0.00% 41 369 0.00% 42 436 0.00% 43 521 0.00% 44 480 0.00% 45 536 0.00% 46 630 0.00% 47 660 0.00% 48 849 0.00% 49 989 0.00% 50 1122 0.00% 51 1340 0.00% 52 1522 0.00% 53 1431 0.00% 54 1696 0.00% 55 1815 0.00% 56 2047 0.00% 57 2455 0.00% 58 2902 0.00% 59 3153 0.00% 60 3975 0.01% 61 4388 0.01% 62 5109 0.01% 63 5676 0.01% 64 6184 0.01% 65 6903 0.01% 66 8029 0.01% 67 8662 0.01% 68 10118 0.02% 69 11476 0.02% 70 13175 0.02% 71 14969 0.02% 72 17345 0.03% 73 20042 0.03% 74 22465 0.04% 75 24874 0.04% 76 28181 0.04% 77 30388 0.05% 78 32813 0.05% 79 37289 0.06% 80 40502 0.06% 81 44695 0.07% 82 50264 0.08% 83 55377 0.09% 84 60672 0.09% 85 67032 0.10% 86 73051 0.11% 87 77417 0.12% 88 81243 0.13% 89 87162 0.14% 90 92311 0.14% 91 99634 0.16% 92 105261 0.16% 93 114430 0.18% 94 121747 0.19% 95 129071 0.20% 96 135927 0.21% 97 141978 0.22% 98 145528 0.23% 99 151122 0.24% 100 156757 0.25% 101 161425 0.25% 102 168699 0.26% 103 175503 0.27% 104 180906 0.28% 105 191560 0.30% 106 198694 0.31% 107 202647 0.32% 108 209587 0.33% 109 212941 0.33% 110 212954 0.33% 111 219040 0.34% 112 223760 0.35% 113 227583 0.36% 114 235357 0.37% 115 240990 0.38% 116 247214 0.39% 117 252536 0.40% 118 257355 0.40% 119 255614 0.40% 120 261350 0.41% 121 262580 0.41% 122 264258 0.41% 123 269582 0.42% 124 276711 0.43% 125 278081 0.44% 126 284967 0.45% 127 291186 0.46% 128 293338 0.46% 129 298217 0.47% 130 298491 0.47% 131 298616 0.47% 132 301020 0.47% 133 302472 0.47% 134 304840 0.48% 135 306941 0.48% 136 310987 0.49% 137 312433 0.49% 138 316476 0.50% 139 321457 0.50% 140 321703 0.50% 141 321613 0.50% 142 326436 0.51% 143 322056 0.50% 144 325674 0.51% 145 327542 0.51% 146 325438 0.51% 147 331012 0.52% 148 330314 0.52% 149 330652 0.52% 150 335567 0.53% 151 48002684 75.12% 63900793 reads passed initial QC criterion=sequence-density sequence-density=0.31 sequence-density-rank=1 fanout-score=23.49 fanout-score-rank=2 prefix-density=0.28 prefix-fanout=23.5 sequence=GGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCTCCTAATCTCGTATGCCGTCTTCTGC criterion=fanout-score sequence-density=0.02 sequence-density-rank=33 fanout-score=267.81 fanout-score-rank=1 prefix-density=0.37 prefix-fanout=15.8 sequence=CATCACCATCCCTGATTGATCTTTGATTCACAACAAGACCAACCAACCGTACAATGTTTACATAACTTGGGATAATCTCACAAGAAAGAAAGATCAATACAAAACTAGTCAGCTCAGGATGTTCCAGGCACGGTAGAACCGTAGAACCATAACAACAAGAGACATATTGCAGATGAGTACTGAAAAACAAAACACAGTACGTATTTACATGGGCAACCTTGGTTGAAGGCAACCTCATCAACGATGCTCGCTCTTCGTCTCTCCACTGTACATCCAGTCATAGACAGTGGGTGTGTTAGGCTGTGGCTTGTCAAAAACATGAGCACCAATATTCTTAGTAGCAAGGTTGCTACCAGGGTGGAACACGCTCCTCCAAACATTGCTACGCGCCGACACTGGGGTTGTAGGGGTCACTGGTGTCGTCGGTGTCCCTGGAGTTCCTGGCATAGTCATGGACCTCTGAAACTTATTAACAGGACTGCTCCCCTCTCCGACGTCAATATCTTT criterion=sequence-density sequence-density=0.21 sequence-density-rank=1 fanout-score=2.79 fanout-score-rank=33 prefix-density=0.21 prefix-fanout=2.7 sequence=ATGTACCCTGAC criterion=fanout-score sequence-density=0.01 sequence-density-rank=38 fanout-score=408.85 fanout-score-rank=1 prefix-density=0.24 prefix-fanout=9.5 sequence=GAGAAAAGATAAGCTAGGCAAGATGGTTTTACTAGACAAGATGTGGGATGATGTTGTTGCTGGACCTCAGCCAGAACGTGGCCTTGGCAAGCTTAGAAAGATCAGCACCAGACCACTTAACAT SRR26075361 testing PE reads STAR mapping to Ensembl genome Started job on | Feb 11 22:39:57 Started mapping on | Feb 11 22:39:58 Finished on | Feb 11 22:47:29 Mapping speed, Million of reads per hour | 510.07 Number of input reads | 63900793 Average input read length | 287 UNIQUE READS: Uniquely mapped reads number | 57804365 Uniquely mapped reads % | 90.46% Average mapped length | 285.84 Number of splices: Total | 46929261 Number of splices: Annotated (sjdb) | 45596919 Number of splices: GT/AG | 46054246 Number of splices: GC/AG | 658453 Number of splices: AT/AC | 49104 Number of splices: Non-canonical | 167458 Mismatch rate per base, % | 0.41% Deletion rate per base | 0.03% Deletion average length | 3.11 Insertion rate per base | 0.02% Insertion average length | 2.61 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 1925408 % of reads mapped to multiple loci | 3.01% Number of reads mapped to too many loci | 339384 % of reads mapped to too many loci | 0.53% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 5.53% % of reads unmapped: other | 0.47% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 4171020 4171020 4171020 N_multimapping 1925408 1925408 1925408 N_noFeature 1627271 56877948 2303736 N_ambiguous 602845 7531 346711 UnstrandedReadsAssigned:55574249 PositiveStrandReadsAssigned:918886 NegativeStrandReadsAssigned:55153918 Dataset is classified negative stranded MeadianReadLen=151 20thPercentileLength=139 echo kmer=135 SRR26075361 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,400 [index] number of k-mers: 62,057,036 [index] number of equivalence classes: 130,681 [quant] running in paired-end mode [quant] will process pair 1: SRR26075361-trimmed-pair1.fastq SRR26075361-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 63,900,793 reads, 56,692,368 reads pseudoaligned [quant] estimated average fragment length: 194.393 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,342 rounds 52401 SRR26075361.ke.tsv 34699 SRR26075361.se.tsv 87100 total ==> SRR26075361.ke.tsv <== target_id length eff_length est_counts tpm Potri.005G200100.1.v4.1 2018 1824.61 9118 80.7798 Potri.005G024800.1.v4.1 1035 841.607 29026 557.507 Potri.004G059700.1.v4.1 961 767.645 99 2.08472 Potri.007G009000.2.v4.1 1416 1222.61 0 0 Potri.003G141000.2.v4.1 2943 2749.61 2480 14.5799 Potri.016G087400.1.v4.1 270 103.607 4310 672.449 Potri.015G069301.1.v4.1 564 372.544 0 0 Potri.010G195200.1.v4.1 1773 1579.61 1035 10.5917 Potri.012G127500.1.v4.1 977 783.607 23389 482.487 ==> SRR26075361.se.tsv <== Potri.001G166300.v4.1 0 Potri.001G448400.v4.1 218 Potri.001G233950.v4.1 0 Potri.001G122700.v4.1 631 Potri.001G212900.v4.1 4 Potri.001G182400.v4.1 0 Potri.001G256600.v4.1 0 Potri.001G040500.v4.1 245 Potri.001G416900.v4.1 0 Potri.001G452600.v4.1 2845 SRR26075361 completed mapping pipeline successfully