Starting /dee2/code/volunteer_pipeline.sh SRR26075362 current disk space = 3052363546624 free memory = 1378745600 SRR26075362 SRAfilesize 6b3d12542b77742d0d128ed15b246692 SRR26075362.sra SRR26075362.sra file validated SRR26075362 is paired end SRR26075362 is conventional basespace SRR26075362 read1 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR26075362_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.6515 37.0 37.0 37.0 37.0 37.0 2 36.587 37.0 37.0 37.0 37.0 37.0 3 36.602 37.0 37.0 37.0 37.0 37.0 4 36.6115 37.0 37.0 37.0 37.0 37.0 5 36.729 37.0 37.0 37.0 37.0 37.0 6 36.6305 37.0 37.0 37.0 37.0 37.0 7 36.5875 37.0 37.0 37.0 37.0 37.0 8 36.6755 37.0 37.0 37.0 37.0 37.0 9 36.6685 37.0 37.0 37.0 37.0 37.0 10-14 36.6654 37.0 37.0 37.0 37.0 37.0 15-19 36.5717 37.0 37.0 37.0 37.0 37.0 20-24 36.5625 37.0 37.0 37.0 37.0 37.0 25-29 36.4765 37.0 37.0 37.0 37.0 37.0 30-34 36.455 37.0 37.0 37.0 37.0 37.0 35-39 36.3848 37.0 37.0 37.0 37.0 37.0 40-44 36.3475 37.0 37.0 37.0 37.0 37.0 45-49 36.2883 37.0 37.0 37.0 37.0 37.0 50-54 36.2333 37.0 37.0 37.0 37.0 37.0 55-59 36.202299999999994 37.0 37.0 37.0 37.0 37.0 60-64 36.0519 37.0 37.0 37.0 37.0 37.0 65-69 35.99839999999999 37.0 37.0 37.0 37.0 37.0 70-74 36.0282 37.0 37.0 37.0 37.0 37.0 75-79 36.0785 37.0 37.0 37.0 37.0 37.0 80-84 36.0797 37.0 37.0 37.0 37.0 37.0 85-89 35.9379 37.0 37.0 37.0 37.0 37.0 90-94 35.841499999999996 37.0 37.0 37.0 37.0 37.0 95-99 35.885400000000004 37.0 37.0 37.0 37.0 37.0 100-104 35.9163 37.0 37.0 37.0 37.0 37.0 105-109 35.8049 37.0 37.0 37.0 37.0 37.0 110-114 35.7348 37.0 37.0 37.0 37.0 37.0 115-119 35.545 37.0 37.0 37.0 37.0 37.0 120-124 35.596199999999996 37.0 37.0 37.0 37.0 37.0 125-129 35.466 37.0 37.0 37.0 37.0 37.0 130-134 35.416399999999996 37.0 37.0 37.0 34.6 37.0 135-139 35.2375 37.0 37.0 37.0 29.8 37.0 140-144 35.118399999999994 37.0 37.0 37.0 27.4 37.0 145-149 35.0514 37.0 37.0 37.0 27.4 37.0 150-151 34.79725 37.0 37.0 37.0 25.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.0 1101 2 0.0 1101 3 0.0 1101 4 0.0 1101 5 0.0 1101 6 0.0 1101 7 0.0 1101 8 0.0 1101 9 0.0 1101 10-14 0.0 1101 15-19 0.0 1101 20-24 0.0 1101 25-29 0.0 1101 30-34 0.0 1101 35-39 0.0 1101 40-44 0.0 1101 45-49 0.0 1101 50-54 0.0 1101 55-59 0.0 1101 60-64 0.0 1101 65-69 0.0 1101 70-74 0.0 1101 75-79 0.0 1101 80-84 0.0 1101 85-89 0.0 1101 90-94 0.0 1101 95-99 0.0 1101 100-104 0.0 1101 105-109 0.0 1101 110-114 0.0 1101 115-119 0.0 1101 120-124 0.0 1101 125-129 0.0 1101 130-134 0.0 1101 135-139 0.0 1101 140-144 0.0 1101 145-149 0.0 1101 150-151 0.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 19 1.0 20 1.0 21 1.0 22 2.0 23 1.0 24 4.0 25 10.0 26 10.0 27 6.0 28 21.0 29 33.0 30 33.0 31 53.0 32 66.0 33 106.0 34 149.0 35 422.0 36 2861.0 37 220.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 38.34586466165413 16.716791979949875 7.593984962406015 37.34335839598997 2 19.3 15.325 35.6 29.775000000000002 3 18.0 18.099999999999998 28.175 35.725 4 20.974999999999998 23.799999999999997 25.724999999999998 29.5 5 25.55 30.85 22.725 20.875 6 21.65 33.324999999999996 22.475 22.55 7 15.299999999999999 28.7 40.125 15.875 8 17.125 28.7 31.574999999999996 22.6 9 17.2 24.75 32.375 25.674999999999997 10-14 19.061906190619062 29.222922292229224 27.61776177617762 24.097409740974097 15-19 19.845 28.37 27.35 24.435000000000002 20-24 20.09 28.904999999999998 26.779999999999998 24.224999999999998 25-29 19.59 28.725 27.91 23.775 30-34 20.18 28.439999999999998 28.09 23.29 35-39 19.89 28.155 28.015 23.94 40-44 19.575 29.715000000000003 27.095000000000002 23.615 45-49 19.89 27.939999999999998 28.015 24.154999999999998 50-54 20.52 28.63 26.85 24.0 55-59 20.53 28.52 27.089999999999996 23.86 60-64 21.145 26.484999999999996 28.52 23.849999999999998 65-69 20.66 27.965 27.435 23.94 70-74 21.325 27.584999999999997 27.515 23.575 75-79 20.265 29.134999999999998 26.965 23.635 80-84 20.945 28.075 26.905 24.075 85-89 21.455 27.905 27.065 23.575 90-94 21.515 27.565 27.065 23.855 95-99 21.45 28.194999999999997 26.200000000000003 24.154999999999998 100-104 21.65 28.305000000000003 26.834999999999997 23.21 105-109 21.895 28.08 26.38 23.645 110-114 21.42 28.105000000000004 26.51 23.965 115-119 21.13 27.644999999999996 27.13 24.095 120-124 22.175 28.63 25.145 24.05 125-129 21.745 27.87 26.650000000000002 23.735 130-134 21.975 27.950000000000003 25.724999999999998 24.349999999999998 135-139 22.46 26.815 26.474999999999998 24.25 140-144 23.44 27.36 25.82 23.380000000000003 145-149 23.84 27.725 24.755 23.68 150-151 24.125 27.575 24.25 24.05 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.5 5 0.5 6 0.0 7 0.0 8 0.5 9 0.5 10 0.0 11 0.0 12 0.0 13 0.5 14 0.5 15 0.5 16 0.5 17 0.0 18 0.5 19 1.0 20 0.5 21 2.5 22 3.5 23 3.5 24 5.5 25 4.5 26 5.0 27 8.0 28 11.0 29 10.5 30 16.0 31 22.5 32 25.5 33 34.5 34 49.5 35 62.5 36 79.5 37 100.0 38 121.5 39 140.0 40 155.0 41 185.0 42 223.5 43 254.5 44 271.0 45 271.5 46 278.5 47 291.5 48 259.0 49 206.0 50 188.5 51 159.5 52 120.0 53 100.5 54 72.0 55 41.0 56 36.0 57 40.0 58 29.5 59 22.0 60 13.5 61 6.5 62 6.5 63 5.0 64 4.5 65 7.0 66 6.5 67 3.0 68 0.5 69 0.0 70 0.5 71 1.0 72 0.5 73 1.0 74 2.0 75 1.5 76 3.5 77 7.5 78 7.0 79 4.0 80 1.5 81 0.5 82 0.5 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.25 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.01 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150-151 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 60.175 #Duplication Level Percentage of deduplicated Percentage of total 1 63.980058163689236 38.5 2 20.56501869547154 24.75 3 8.2675529705027 14.924999999999999 4 3.199002908184462 7.7 5 2.4927295388450355 7.5 6 0.8309098462816784 3.0 7 0.1661819692563357 0.7000000000000001 8 0.1661819692563357 0.8 9 0.12463647694225177 0.675 >10 0.2077274615704196 1.4500000000000002 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GCCCGATACCACCTGCGATTCGCCTTTAACGATACTCTCAAGCATCAAAT 17 0.42500000000000004 No Hit GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGTCCAGAATCTCGGGG 11 0.27499999999999997 TruSeq Adapter, Index 4 (97% over 39bp) GCTTACAGAAGGATATGAGCCAAGACGCCATATGCAGACATCCAAGGTGA 10 0.25 No Hit AGCTGTTCCTGGGTAACTTGTCCAGGTGTAGCAATAGCAATTAGTTCCGC 10 0.25 No Hit GTTCATTGTTTCATCCCTTAATGCTTGAAGAATGGCCTGCACTGTGAACT 10 0.25 No Hit TAATCAACCACCTTCTCCGCTATTGGAAAGTTGGTCTTCGCCTTAGCACC 9 0.22499999999999998 No Hit GTAGTAGTAGCCCTAGTAGTATTGGAAGAGAGCTCTCTCTCTCTCCCCCA 9 0.22499999999999998 No Hit CTCAGATGTTTCTCGAATTTCAATCTCTGATGAATCACTAATGCTGGTCT 9 0.22499999999999998 No Hit ACGTACTAATCTAGAAGTTTGCCTAATAACTCCATAACGACAAATCAGAA 8 0.2 No Hit CTCTTCTTCTTTCTTCCCCAGGAAATCAAACAACCCGCGATCCTTGGTCT 8 0.2 No Hit GTCGTACATTCCAAAGTAGAGACCACGATAGACAATGATTCCAACACAGG 8 0.2 No Hit ACCATTCATGCGCTTGCCCTTTGATCTCTCAATAGATGCTACTTTCTGAA 8 0.2 No Hit CAAGAGCATGGATCACAGGTGCAGCTTGATCCACACTTGCAGCCATTCTC 7 0.17500000000000002 No Hit CCTGAAAAGTGGAGCCAGATCCACCTGTTGGTTGTTCCATGTTGTCAACT 7 0.17500000000000002 No Hit CCTAGCAAGCCCACTGGCAACAATACTCTTGGCGGCTTGCCTGACAATGT 7 0.17500000000000002 No Hit ACGAGCAAGACCATTTGCTACGATGCTCTTGGCAGCTTGCCTAACAATGT 7 0.17500000000000002 No Hit ATTTCCTCCAAGTGAGATAGCTGAAAATTGGTTTCTCTGGAACAGGTATG 6 0.15 No Hit GCGGCCGATGCAACCAGTGCACAACATAAAGTCAGATAAACCTGCTTGAG 6 0.15 No Hit CTGACCTGTGCATCTTGAGGAATATTTGGACATTCAATGCATGTAGTTCC 6 0.15 No Hit GTTCAGGAACTCGGGAAAATCAATTGTACCATTTCCATCAGCATCAACCT 6 0.15 No Hit TGATCCTTCTATCTTGGGCTTGGCACTCAAATACGCAAGCATCTCTTTGG 6 0.15 No Hit CTTCTCGTATTTCTTCTCCTCATGCCTCCTCTTCTTTCGGCTGCTTTTGT 6 0.15 No Hit GTGTGGACAAAATTTCTGCATCATGCTTGTGGAGTCTAGAATGCAGAAGA 6 0.15 No Hit AATTCATAAACAATGTAGACATCATTGAAGTTTTCTGTCTGGGGAGGCCG 6 0.15 No Hit GTGTCCACGAGCAACCCTGGCATGTCAAACACTGCTTCCTCGAAGAAGAC 6 0.15 No Hit CATATTATTATAATTCAGTTCTGGGTTGCTCAAGTAACTGAAAGACTGGC 6 0.15 No Hit GCAGAGATGTGGAAGCTGAACTAAGGCCCAGGTTTGAGAGAAGAACTCCA 6 0.15 No Hit CGGTGGTTTCACGGTGGTGGGTGCAGGAGCAGAGGCTTTTGGTGGTGGGG 6 0.15 No Hit CACCAATTCCATTCCTCTTATTTTCACGAGCTATAGCTTCAGCTCTATCC 6 0.15 No Hit CTAGGATACAAGGAAAACAAAAGGATCCGTTCATTTAATCTGAAGGCTGA 6 0.15 No Hit CTCCCATTCTGTGGCTTAACTGGAGGTGGCTGGTCAATTGTAACAACATG 6 0.15 No Hit ATTGCAAACCAGTTTCTCAAGAGAAACAGACCATCACAAGACAGTAAACA 6 0.15 No Hit CAGCAAAAGAAAATAATTCCAGTTCAGATGCATCCCAAATACAATTGAGG 6 0.15 No Hit CTATAATTCAGGGTTAAATCCAGCCCAGTAAACAATTTTGCAGCTACCAG 6 0.15 No Hit GTTGGATACACCTCTGGGGCATATATTGCAGCAACTGTGAAGGTTCCTAT 6 0.15 No Hit GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGTCCAGAATCTCGGGT 6 0.15 TruSeq Adapter, Index 4 (97% over 39bp) GGCTAACCCAAACTAAGGCCTCAGTTGCCAGGTTTTTGAATGACAAAAGC 5 0.125 No Hit AGCACCTTATTAACCACAGAGAACTCGTAGAAAGTGAACTTAAAATCAAA 5 0.125 No Hit GTGTTGTTTTTTATCAAGATCACCGCTTCCTATCAAGCACTCTTTTGATT 5 0.125 No Hit CTTCACAAAAATCTGCATACCCCCTCGGAGACGGAGGACAAGGTGAAGGG 5 0.125 No Hit AGCTGAGAATCCTAATATGTTCAATCAATCTTCCATGGGTGAAATAATCC 5 0.125 No Hit AGCGTCTTATACAGTTCCACTAGTTTAGGAGTGAATTCACGGCACATCCT 5 0.125 No Hit ACACGATCCAAGGATAGAGGATCAAACGGAACAATCTACATATCCTCAAT 5 0.125 No Hit GGATTGGAATAATTGGTGTTGGACGAAAGAGCTTGGGTGACATTTTATCC 5 0.125 No Hit CTCGATTCTGAAAACACCTGGCTTTCGATAAAATCTTTGTTAAGTTTGCT 5 0.125 No Hit GCCAGAAACATGATCTTAATAGATAGAGGTTGCTTCACTTAAAGCACTTG 5 0.125 No Hit GCCTGATTGGTCTTCTTGACAGTGACACCACCAGAACCCTTCTTTCCTCG 5 0.125 No Hit CACCAAGAACCACAAGAATATCATTCATTACAAATAAAAAGCCACTTAAA 5 0.125 No Hit CTGGGTTCTTTGTGGGCAACTGGGCAGCAACATCTGAAGCAGAGAGTTTG 5 0.125 No Hit ACCGAATGTTGCCCGTCCATTTCTATTGAATCTGATTCCATGGTTCCCGA 5 0.125 No Hit GCTTTGAATTCCTTCTTGGAGCACCAGAATCACCCGATCCGTTGCCTCCA 5 0.125 No Hit CCCTCGATCACTGCTCGGACCTCAGAAGGAGGATCAAATTCCTTCACTTC 5 0.125 No Hit AGCTTTAGCTGTATCAGGCTTCTGAAGTAGGACAGTTTTAGTTGCTTTCA 5 0.125 No Hit CAACTTCCCACAGTATCCCATTCTCAATCTCCTTGTATGGGAACGAATCC 5 0.125 No Hit GCCAGAACTAACCCCTCAAAAGGGTACAAGTAATAGCCTCTACTCTTTCT 5 0.125 No Hit ACTCAACCAGCTTTAACATTGCTGAAGATGGCAACTCCTTGCAGAAGCGT 5 0.125 No Hit GTTCATATCAAGTGTTTCTGAGGCATAATCTATCCCAGAATCTTCTCCTG 5 0.125 No Hit ACAAGACAGAGAGCGAGATATTCTTCTTCAGTAGGCTGGTGGTCGAGACG 5 0.125 No Hit GATTGATAGAACCTTCGTTAGATGCACCCCGAGGCTTCTATGAACGCCTG 5 0.125 No Hit CCGAGCAGATAGATGTCCTTCATAGCTACAGTGTACCGAGAATGTAATTA 5 0.125 No Hit GGTGCTTTGAATGACCTCTCCTCCTGATAGCACCATTGTCTTCATCGCTT 5 0.125 No Hit GCTAACTTTCTCAGCTCTAGTACATAACACTCCAAATACACCGCGTATAA 5 0.125 No Hit GTTGGAATTTGTTCCTCAAGTATAACGGTCACGTTCTTAGAGTTGATCAC 5 0.125 No Hit GACAGAATAAGATATTATTATACAAAAAACTTAAGAGCGACGTAGAAAAT 5 0.125 No Hit TTGGAATGTAGGATTTTTGCCTCCATCAGTGCAGGTTCTTGTGCGAAACT 5 0.125 No Hit TGGCAGGTGTGACAGTCAAGGACAGGGGCATATCCATTCCCAATTTGACC 5 0.125 No Hit AGCATCCTCCATCCAATAAGCAAAACACCTGTGCTTCCCATTGTTACTAA 5 0.125 No Hit CCCTAATCATACTTCTCTCTCCAAGAATAAACAAGAAAGAGAACCCAAGA 5 0.125 No Hit CCTCAAAAAACCAAAAAAAAGGGGGATATGTTATTTTAACCCTTTAGCTT 5 0.125 No Hit CTCATCCACACAAACATCAAACGACAAAACTCTGACCCTGTCTCAACTCT 5 0.125 No Hit CATAATTACCCTTCTTTGCAGGCACAAAATTAATCACAATCTTATCAGAA 5 0.125 No Hit GCCCACCTTTCCATGTACTAATTTAGGTGGCCTGTCATGTAAGAGAGGTG 5 0.125 No Hit CAAGCGTCTGCGATTCCAGTTTTGCCTTTTCAATTGCAGCTAGCTCGTCA 5 0.125 No Hit GTCCCCTGCACTAGAAGTTGACCTCTGTGAAGGCATTGCTGAACCTGTAG 5 0.125 No Hit GATGAATGTTGTGTGGGCAGGGTGACCCAGTTCAGAATGCCAATATATAC 5 0.125 No Hit CCCCAATCACCGTCAATTTCTTGTCCTTCATGTCCATGGATATTGAATCC 5 0.125 No Hit GACCGATTCGTGCCACTAGAAGTTAAATGGCAAGACCACATCATAACCGA 5 0.125 No Hit CTTGATGAACCACTTGGAACTTGCTCCGATGATGCCATACTTTGTGAGGA 5 0.125 No Hit ATCCGCTCTTGGTTTTGAGCTCCTGTCATCATTGCTTGACTTAGATTTTG 5 0.125 No Hit GCATTCTTATGATGAACCTGGACATGTGTTTCCTTGTTGACGCAGCAGAT 5 0.125 No Hit GTCTTGTTGCCAATATTCCACACTCTTGTCAAAATCAACTCGGCCCGTCA 5 0.125 No Hit CTTTAGGACATAATCAGTGTACAATTCATATATGACTTTCAAGAGGCCTT 5 0.125 No Hit CTCCATGACAAAATCGAGAACCCAAACTCGTGGTGCTGATGTAACAGTTA 5 0.125 No Hit GTCAAATGAAAGCTCAAACCCGTAAAGGTAATCATGGTTCAGTTGCTGAA 5 0.125 No Hit CTCAAATCCAGCACTATCAGCACAAAGCTGATTGAGTGGCCGCTCTGATG 5 0.125 No Hit CTTGATCGTTGGCTATGATTTCCACTCTGTCATGTTGCCACACACCGACA 5 0.125 No Hit CTCAAGAGGACGGACATTGGAGTCTACCAGATTCACCTTCCCCTTGCCAG 5 0.125 No Hit CCTCAATTGCTGTTTCTTATAGTGATCCAAGGATTCGTCCACAGGCTTCA 5 0.125 No Hit ACAACTCTATAAACCCAGAAAACAAAAAATTAGCATGACATTACACTATC 5 0.125 No Hit GGTCTGTCTCCTAGGCGTTCCCGTCCCTTCAGCCCTAACAATTCAATAAC 5 0.125 No Hit CATCACTTGGCTTTCCATCCCATACCTGTTTCTTGAGCTGCTGTTGCTTG 5 0.125 No Hit CTCATCTGTCAGCTTCTCCCCGAGATTTGTCATGACATGACGCAACTCAG 5 0.125 No Hit GGTAGCATGCCTGATGATGGTATCCCATGTCCTGTTTGAGATACCAGATC 5 0.125 No Hit CTCCCTTTTTACATGGACATCACTGCCAAGAACACTCTCCAGTACATTGC 5 0.125 No Hit TGGTGGTAGTGCTGGTAAAATTTTCATCTTTCTTCAAGATATCAAAAATG 5 0.125 No Hit CTCCACTTACTCTATCTGCAGCAGCTTGTAGGGTTACATGATCCCCCCAT 5 0.125 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.025 0.0 0.0 0.0 0.0 5 0.025 0.0 0.0 0.0 0.0 6 0.025 0.0 0.0 0.0 0.0 7 0.025 0.0 0.0 0.0 0.0 8 0.025 0.0 0.0 0.0 0.0 9 0.025 0.0 0.0 0.0 0.0 10-11 0.025 0.0 0.0 0.0 0.0 12-13 0.025 0.0 0.0 0.0 0.0 14-15 0.025 0.0 0.0 0.0 0.0 16-17 0.025 0.0 0.0 0.0 0.0 18-19 0.025 0.0 0.0 0.0 0.0 20-21 0.025 0.0 0.0 0.0 0.0 22-23 0.025 0.0 0.0 0.0 0.0 24-25 0.025 0.0 0.0 0.0 0.0 26-27 0.025 0.0 0.0 0.0 0.0 28-29 0.025 0.0 0.0 0.0 0.0 30-31 0.025 0.0 0.0 0.0 0.0 32-33 0.025 0.0 0.0 0.0 0.0 34-35 0.025 0.0 0.0 0.0 0.0 36-37 0.025 0.0 0.0 0.0 0.0 38-39 0.025 0.0 0.0 0.0 0.0 40-41 0.025 0.0 0.0 0.0 0.0 42-43 0.05 0.0 0.0 0.0 0.0 44-45 0.05 0.0 0.0 0.0 0.0 46-47 0.05 0.0 0.0 0.0 0.0 48-49 0.05 0.0 0.0 0.0 0.0 50-51 0.05 0.0 0.0 0.0 0.0 52-53 0.05 0.0 0.0 0.0 0.0 54-55 0.05 0.0 0.0 0.0 0.0 56-57 0.05 0.0 0.0 0.0 0.0 58-59 0.05 0.0 0.0 0.0 0.0 60-61 0.05 0.0 0.0 0.0 0.0 62-63 0.05 0.0 0.0 0.0 0.0 64-65 0.05 0.0 0.0 0.0 0.0 66-67 0.05 0.0 0.0 0.0 0.0 68-69 0.05 0.0 0.0 0.0 0.0 70-71 0.0875 0.0 0.0 0.0 0.0 72-73 0.15 0.0 0.0 0.0 0.0 74-75 0.16249999999999998 0.0 0.0 0.0 0.0 76-77 0.21250000000000002 0.0 0.0 0.0 0.0 78-79 0.275 0.0 0.0 0.0 0.0 80-81 0.3 0.0 0.0 0.0 0.0 82-83 0.3625 0.0 0.0 0.0 0.0 84-85 0.5125 0.0 0.0 0.0 0.0 86-87 0.6 0.0 0.0 0.0 0.0 88-89 0.65 0.0 0.0 0.0 0.0 90-91 0.7250000000000001 0.0 0.0 0.0 0.0 92-93 0.9875 0.0 0.0 0.0 0.0 94-95 1.375 0.0 0.0 0.0 0.0 96-97 1.65 0.0 0.0 0.0 0.0 98-99 1.8625 0.0 0.0 0.0 0.0 100-101 2.0875 0.0 0.0 0.0 0.0 102-103 2.425 0.0 0.0 0.0 0.0 104-105 2.875 0.0 0.0 0.0 0.0 106-107 3.425 0.0 0.0 0.0 0.0 108-109 4.0625 0.0 0.0 0.0 0.0 110-111 4.5375 0.0 0.0 0.0 0.0 112-113 5.0125 0.0 0.0 0.0 0.0 114-115 5.5875 0.0 0.0 0.0 0.0 116-117 6.1 0.0 0.0 0.0 0.0 118-119 6.7375 0.0 0.0 0.0 0.0 120-121 7.574999999999999 0.0 0.0 0.0 0.0 122-123 8.350000000000001 0.0 0.0 0.0 0.0 124-125 9.1125 0.0 0.0 0.0 0.0 126-127 10.1875 0.0 0.0 0.0 0.0 128-129 10.8875 0.0 0.0 0.0 0.0 130-131 11.575 0.0 0.0 0.0 0.0 132-133 12.2 0.0 0.0 0.0 0.0 134-135 12.9875 0.0 0.0 0.0 0.0 136-137 14.087499999999999 0.0 0.0 0.0 0.0 138-139 15.024999999999999 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TGGCAGC 10 0.006830828 145.0 3 CTGGGTA 10 0.006830828 145.0 9 CAACTCC 10 0.006830828 145.0 5 GCAGCTG 10 0.006830828 145.0 5 GGCAGCT 10 0.006830828 145.0 4 AACATCG 10 0.006830828 145.0 145 CAGCTGG 10 0.006830828 145.0 6 AGCTGGG 20 3.5877043E-4 108.75 7 GGGGGGG 35 0.0035366106 20.714287 140-144 >>END_MODULE SRR26075362 read2 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR26075362_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.15825 37.0 37.0 37.0 37.0 37.0 2 36.037 37.0 37.0 37.0 37.0 37.0 3 36.02 37.0 37.0 37.0 37.0 37.0 4 36.132 37.0 37.0 37.0 37.0 37.0 5 36.2175 37.0 37.0 37.0 37.0 37.0 6 36.123 37.0 37.0 37.0 37.0 37.0 7 36.071 37.0 37.0 37.0 37.0 37.0 8 36.2065 37.0 37.0 37.0 37.0 37.0 9 36.141 37.0 37.0 37.0 37.0 37.0 10-14 36.0068 37.0 37.0 37.0 37.0 37.0 15-19 36.0397 37.0 37.0 37.0 37.0 37.0 20-24 35.879999999999995 37.0 37.0 37.0 37.0 37.0 25-29 35.73350000000001 37.0 37.0 37.0 37.0 37.0 30-34 35.6349 37.0 37.0 37.0 37.0 37.0 35-39 35.5692 37.0 37.0 37.0 37.0 37.0 40-44 35.475100000000005 37.0 37.0 37.0 37.0 37.0 45-49 35.4865 37.0 37.0 37.0 37.0 37.0 50-54 35.29430000000001 37.0 37.0 37.0 37.0 37.0 55-59 35.366 37.0 37.0 37.0 37.0 37.0 60-64 35.387800000000006 37.0 37.0 37.0 37.0 37.0 65-69 35.321600000000004 37.0 37.0 37.0 37.0 37.0 70-74 35.2533 37.0 37.0 37.0 37.0 37.0 75-79 35.091300000000004 37.0 37.0 37.0 29.8 37.0 80-84 35.22670000000001 37.0 37.0 37.0 37.0 37.0 85-89 35.17100000000001 37.0 37.0 37.0 32.2 37.0 90-94 35.0529 37.0 37.0 37.0 27.4 37.0 95-99 35.1541 37.0 37.0 37.0 32.2 37.0 100-104 35.060199999999995 37.0 37.0 37.0 27.4 37.0 105-109 35.0757 37.0 37.0 37.0 27.4 37.0 110-114 34.89730000000001 37.0 37.0 37.0 25.0 37.0 115-119 34.9306 37.0 37.0 37.0 25.0 37.0 120-124 34.7444 37.0 37.0 37.0 25.0 37.0 125-129 34.857099999999996 37.0 37.0 37.0 25.0 37.0 130-134 34.7383 37.0 37.0 37.0 25.0 37.0 135-139 34.67655 37.0 37.0 37.0 25.0 37.0 140-144 34.58595 37.0 37.0 37.0 25.0 37.0 145-149 34.60415 37.0 37.0 37.0 25.0 37.0 150-151 34.139125 37.0 37.0 37.0 25.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.0 1101 2 0.0 1101 3 0.0 1101 4 0.0 1101 5 0.0 1101 6 0.0 1101 7 0.0 1101 8 0.0 1101 9 0.0 1101 10-14 0.0 1101 15-19 0.0 1101 20-24 0.0 1101 25-29 0.0 1101 30-34 0.0 1101 35-39 0.0 1101 40-44 0.0 1101 45-49 0.0 1101 50-54 0.0 1101 55-59 0.0 1101 60-64 0.0 1101 65-69 0.0 1101 70-74 0.0 1101 75-79 0.0 1101 80-84 0.0 1101 85-89 0.0 1101 90-94 0.0 1101 95-99 0.0 1101 100-104 0.0 1101 105-109 0.0 1101 110-114 0.0 1101 115-119 0.0 1101 120-124 0.0 1101 125-129 0.0 1101 130-134 0.0 1101 135-139 0.0 1101 140-144 0.0 1101 145-149 0.0 1101 150-151 0.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 13 4.0 14 10.0 15 18.0 16 13.0 17 9.0 18 7.0 19 12.0 20 8.0 21 3.0 22 9.0 23 11.0 24 14.0 25 15.0 26 16.0 27 18.0 28 20.0 29 29.0 30 32.0 31 39.0 32 56.0 33 122.0 34 245.0 35 828.0 36 2269.0 37 193.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.08627156789197 21.255313828457115 10.602650662665667 23.055763940985248 2 29.049999999999997 25.775 26.424999999999997 18.75 3 24.8 29.125 29.375 16.7 4 27.125 34.325 19.925 18.625 5 28.075 36.0 19.775000000000002 16.150000000000002 6 24.275 37.875 20.4 17.45 7 24.65 20.65 36.199999999999996 18.5 8 23.599999999999998 25.025 27.625 23.75 9 23.75 25.224999999999998 28.799999999999997 22.225 10-14 25.66 29.220000000000002 24.84 20.28 15-19 25.900000000000002 27.534999999999997 26.155 20.41 20-24 25.52 27.639999999999997 25.955000000000002 20.885 25-29 26.919999999999998 27.565 25.629999999999995 19.885 30-34 26.3 27.400000000000002 26.58 19.72 35-39 25.985000000000003 27.384999999999998 25.825 20.805 40-44 25.345000000000002 28.13 25.825 20.7 45-49 26.150000000000002 27.189999999999998 25.855 20.805 50-54 24.545 27.85 27.525 20.080000000000002 55-59 25.405 27.22 27.12 20.255000000000003 60-64 25.324999999999996 27.950000000000003 26.669999999999998 20.055 65-69 26.05 26.08 27.555000000000003 20.315 70-74 24.69 28.57 25.705 21.035 75-79 24.565 28.985 26.035000000000004 20.415 80-84 25.545 27.46 26.855 20.14 85-89 25.36 28.34 26.455000000000002 19.845 90-94 25.595000000000002 27.24 26.915 20.25 95-99 26.155 28.095 26.27 19.48 100-104 26.584999999999997 28.360000000000003 25.56 19.495 105-109 25.85 28.15 25.790000000000003 20.21 110-114 26.645000000000003 27.495000000000005 26.69 19.17 115-119 26.974999999999998 27.04 26.76 19.225 120-124 25.900000000000002 28.410000000000004 26.215 19.475 125-129 27.48 27.62 26.290000000000003 18.61 130-134 26.245 28.415000000000003 25.605 19.735 135-139 27.66638331916596 28.40642032101605 25.351267563378173 18.575928796439822 140-144 27.554133119967993 27.044056608491275 26.979046857028553 18.42276341451218 145-149 28.68217054263566 28.542135533883474 25.026256564141036 17.749437359339833 150-151 28.116014501812725 27.17839729966246 26.378297287160894 18.32729091136392 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.5 6 0.5 7 0.0 8 0.5 9 1.0 10 0.5 11 0.5 12 1.5 13 1.0 14 2.0 15 2.5 16 1.0 17 1.0 18 1.0 19 1.5 20 1.5 21 0.5 22 1.0 23 1.5 24 1.0 25 1.5 26 1.5 27 1.0 28 5.0 29 7.0 30 6.5 31 9.5 32 7.5 33 12.0 34 34.5 35 52.0 36 70.0 37 94.0 38 107.5 39 120.5 40 163.5 41 205.0 42 229.5 43 280.0 44 295.5 45 299.0 46 263.0 47 246.5 48 282.0 49 237.5 50 171.0 51 120.5 52 115.0 53 121.5 54 81.5 55 58.5 56 51.5 57 29.0 58 20.0 59 26.0 60 20.5 61 8.0 62 5.0 63 3.5 64 2.0 65 1.0 66 0.5 67 1.0 68 1.5 69 1.5 70 3.5 71 3.0 72 0.5 73 0.5 74 1.0 75 1.0 76 1.0 77 2.0 78 2.5 79 3.0 80 3.5 81 4.0 82 4.5 83 2.5 84 2.0 85 3.0 86 3.5 87 3.0 88 2.0 89 3.0 90 5.0 91 4.0 92 1.5 93 3.0 94 3.0 95 1.5 96 1.5 97 2.5 98 2.5 99 3.5 100 17.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.025 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.005 140-144 0.015 145-149 0.025 150-151 0.0125 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 61.9 #Duplication Level Percentage of deduplicated Percentage of total 1 67.20516962843296 41.6 2 19.022617124394184 23.549999999999997 3 7.310177705977383 13.575000000000001 4 2.988691437802908 7.3999999999999995 5 2.221324717285945 6.875000000000001 6 0.6462035541195477 2.4 7 0.20193861066235863 0.8750000000000001 8 0.12116316639741519 0.6 9 0.12116316639741519 0.675 >10 0.12116316639741519 1.05 >50 0.04038772213247173 1.4000000000000001 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG 56 1.4000000000000001 No Hit GCAAATCCTTCAAACCCGTGAAACGGCAAAACCTTTACAATGAAACGACA 17 0.42500000000000004 No Hit GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGT 15 0.375 No Hit ATTCCAATCTTCCACTAGAAATATCCAAACGAGAGTTGATCACCCTCCAT 10 0.25 No Hit GGGGAGCTGTAAAGAAGTTGGAGCCTGGTTTACGTGCATATCAGAAACAT 9 0.22499999999999998 No Hit AAGAAAGCAAGTGGTGATCATGACAAGAAGGAAATCCATTATAGGGGAGT 9 0.22499999999999998 No Hit GAGAAGTTCGATCCTTCAAGATTTGAGGTTGCACCGAAGCCCAATACATT 9 0.22499999999999998 No Hit GGAGGTGGCTCATTCTCTGCAGGGGGGCCTGGAAAAGGGATGCACTCACG 8 0.2 No Hit GCTCGAACCCGTTTGGCCAATGATGCCAAGGCTGCAAAGAAGGGAGGAGG 8 0.2 No Hit AAATGATGTCTTCTTCGAGGAAGCAGTGTTTGACATGCCAGGGTTGCTCG 8 0.2 No Hit GAATGATATCTCTCGAGTAAGTTCAGATGAAGATTCCGCTCCGGCCATCT 7 0.17500000000000002 No Hit CTAAGCCCAATCCCCGGATTCATGCAATGGTTGCTATCTAAGTTGGCATC 7 0.17500000000000002 No Hit TGGCCGTTTCGTCATTGGTGGTCCTCATGGTGATGCTGGTCTCACCGGGC 7 0.17500000000000002 No Hit GACTTGTCTGATACTCAACGAAAGCATGCTTTGGCTATTGAACGATTTGC 7 0.17500000000000002 No Hit GGTTGCAGCTGTGGATCTGACTGCAAGTGCGGCAGTGGCTGCAAATGTGG 7 0.17500000000000002 No Hit AGTTAAGAACGAAGGATGGGGACGATTGTATAGCGGATTAGCGCCATCGA 6 0.15 No Hit GTTGAGAACACCACAGGAATCTCAGCATGGAGATGCAGTAATGGAAGCCA 6 0.15 No Hit CGTGAGTCATCAAGCCGAAATGTGTGATTCAAAAGCTGGGGCAGAGCTTC 6 0.15 No Hit ATTTGACATGTCTATGTGAGTGCTTCGTCCTTGTTGCTCGTACATGTCAT 6 0.15 No Hit ACTCCACTTCAGGCTGTGAAATTGCTCGCTTTGTATCTCTCAAGCCCTGA 6 0.15 No Hit CAAGTACTCTTCCATCTATTAGCAAGGAGGCTTCACCTGAGCCAGTTAAG 6 0.15 No Hit GCAAGGGCGTATGATGCTGAGGCGCGTAGAATTCGTGGCAAGAAAGCTAA 6 0.15 No Hit GGAAACTGTGGCTAGAGGACCAAGCTGGAGAGATCTTTTTGAACCAGGTG 6 0.15 No Hit GAGAGCGAGCCGCTAGCTAGCTGCTCTGCAGGACGCTGTTCTTCTTGAGA 6 0.15 No Hit CGCACGTTTTCTCCATCTCTCAGGTTTTTATCTCCTTCTAGGTTTCATTT 6 0.15 No Hit AGATTTGGCCGCAAGCTTTCAATGGCATTCATGTTTGTCTTAGCTTGTAT 6 0.15 No Hit CAGCTTATGAGTTCATTAAAGTACACGAAGCTTATGAAACTCTGTCGGAC 6 0.15 No Hit GTTTGATTAAAAACCATTCTCGATAATGATCCACTGACACGTGTAGGCTG 6 0.15 No Hit GAAAGTTTTTACAGGCATGTAAGAGGTTTGATTCTCAAATGATGTGATGC 6 0.15 No Hit CTCGTTCATCAGGCAAAGCTGTTGTCGGATGGCTGAAGCTCAAGGCGGCC 6 0.15 No Hit GAATTTTTGAAGAAATTTGCTGACACGGAGAGGTTGAACTGGGGCCAATG 6 0.15 No Hit AAAGAGAGAACTTTGAAGTAGTCCTACTATCTCTAGACGACGAAGAAGAA 5 0.125 No Hit AGCACTCAACACCATATCTATGACCCACCCTCACATTCCAGTCGAACACC 5 0.125 No Hit TTTTTTTTTTTCCTGCAAAATTGGAAAGCATGCTTACAATAAATGTAGCT 5 0.125 No Hit GTGCAACAAGACAGAGGTGGAGATTGGGGTGCAACACGTGAACTGTACAG 5 0.125 No Hit GCTCAAGGAGGTGGAAGCTGTTTGCAACCCAATCATCACTGCCGTGTACC 5 0.125 No Hit AAGGCCAAGCACGAACAAAACTCTCTCTCCCTCACTCTCTCAATACCTCT 5 0.125 No Hit GAGAACCCAAAAGCTGGAAACACAGGGACTAGGAATCATCAAGAGGCACA 5 0.125 No Hit GCTAGAGGATTGCTCAAGTGAAGTTTGCAGCGATGATTGCAAAACTTTCC 5 0.125 No Hit ACGACGGTAGCGTTTGGGCCCTAAGCGCCTCCTTCCCTCAGTTCACCCAA 5 0.125 No Hit TGCCCTTGTTGACTTCTGCAAATTTAAGTGCACAGCTGGTTCTTGGTAAC 5 0.125 No Hit GCTACCAAGAATTCTGTTGCACGGAGCAGCAGAACTGTGTCCAATGGCTC 5 0.125 No Hit CCTGAAGTTCTTGCTCTATTCAGACCTGTGTAGCGGCATTAACCTCTTAG 5 0.125 No Hit GTTATGGTGAGGATGGTCGTGGAATGAATGGTCTGTCTAAGGCTGCTATT 5 0.125 No Hit CTCTTGTACGCAATGAACTTCCCTGAGATTAACCCTAACCATGATTTCTC 5 0.125 No Hit ACCAGGATAGCAATGTCTCTGACATGAAGTTTGGATGGCTTAATCTCTTT 5 0.125 No Hit AGAAGAGAATGCTAAGAAGAATCAAACCATAAGCTCTATCTTGGTTTCCA 5 0.125 No Hit AGAAAAGTCAGCTCCACCAATTCTGTTGCTGCGCTTGATTCCAATAAAGG 5 0.125 No Hit GGTAGATGTAACAACTGTTCCAACGAAACGTCTTTACGTAGCACCAGGTT 5 0.125 No Hit CGAAAGAGAGGAGCTCTTCTGCGCAATCATGTTGGTCTATCAAGATCTTC 5 0.125 No Hit GATCAATATGATCATGCAGACATGGGAGGCGATATTCTTGAAGATGACAT 5 0.125 No Hit GTTTTATTTCAAAGTCAACCGTATTGTGGATAAATATTACAAGCCTCCTC 5 0.125 No Hit CCTAAGAAAATCTCTGGTCTAGAGCCACTTAGGCAACGTTTAGCTAATGA 5 0.125 No Hit GATCACCGCGGAGAGTCGTATCAGAAACATCACCCTCCACACTTCCGTGA 5 0.125 No Hit TTTATTACCTTCATCTGTTGAATGTTTGACTTAAAAAGTACCACAGGTGC 5 0.125 No Hit CACCAACTGTTGGCTGTTTAGGCATTGAACTCCTCGAAGCTGATACCTTT 5 0.125 No Hit GCCACAGCCGCGTTTGAACAATAACAGGCCTGAAGGCCTTCCCATGTCAA 5 0.125 No Hit CAGCAAAACATGCATATTAGATGATTTTGGGTTTTACGAGGATCGGGAGA 5 0.125 No Hit CCAAGACACAGGATCGCCATTTGCTTAATGATTATTTTTTCAGTGCGATA 5 0.125 No Hit CTCAAACGTGGTTTTGTTGCTTCCAACTCTAAGGATGATCCTGCCAAGGA 5 0.125 No Hit ACTTGTTCCACTTCTCTCCTCTTTTGGCATCTCCTTTCTTTCCGTGCTTG 5 0.125 No Hit GTGCAATTGTTCTAACAACTCATTCAATGGAAGAAGCCGACATTCTAAGT 5 0.125 No Hit CCGAAGATGGCCCCCATAAACCCGGGTCGGTCGGACGACCCATTGGGCAG 5 0.125 No Hit GTGGAATTCTTTATCTTGACAATGGAGCTCTCAAAATTGTGCCTTGGGAT 5 0.125 No Hit TGATGAGTTTGTGAGTGATTCAGTGAGGTTCTCCAAAAAATCTACACCGT 5 0.125 No Hit ACTATCTGAGGTGGCTCGCAAGTGGAAGAACCACAAATGCACTCAGGATT 5 0.125 No Hit TGGGTGTTGTGATCGTGTTGCCCCCCATAAACTTGAGCCGTGCCACTGAT 5 0.125 No Hit GGTTAGCACAAAACTTCTCTCCTCCTCCTCCTCCTTCCCCTTCCCTTCCC 5 0.125 No Hit GTTAAGACAATGGCCTCGAAGAAATCTGCAATCGTATTACCTGGTTCAAA 5 0.125 No Hit GGTAACAAGAAGCTTGATGCAGCATATCAAGAGGCTGGGCAGAAATCTGG 5 0.125 No Hit GTCGAAAGATTTCCTTCTCTCTAAACAATGGAATCGTTCGCTTCCTTCTT 5 0.125 No Hit TGATTCTTCAAGCCATGATGATGAAGCTGTCAAGAGGAGAAGCCGATCAA 5 0.125 No Hit GTTTCCAAAAAAGGCGAAAAACAGAGTATTGTACCTGAAGACATTCGAGC 5 0.125 No Hit TACCATTAGATCTAATCATAATGGCCGGAAAAGGAGAAGGACCGGCTATC 5 0.125 No Hit AGAAACCTTGGAGCCGAGAAGAAATTGAGCTTCTGAGGAAAGGAATTCAG 5 0.125 No Hit CAAAAAGAAGGTTCAAAGGAGTATCGAAGACCAATCTGTTCTAGTGGCAA 5 0.125 No Hit GCCTTTGGGAAGATGCTAGTGATCGGCTCAGAGCCTCCATTCAAGGTCAA 5 0.125 No Hit GTTGCCTTTATTCAAATGCGCAAGATAGATGGAGACCCTTGTCCCAAAGA 5 0.125 No Hit GAAGCAGTTATTAATGAAGCTTTTCGCGATAATTTTCAGCATGAGAAACG 5 0.125 No Hit GCCAAGTTGGCCATCAGTCGTGCCATTTCTGTTTGAGGTTATTGCTATTT 5 0.125 No Hit GGTTTGAGAACTGGGCCCATTTAAGTAACGTTCTGCACCAGATGCGGCCT 5 0.125 No Hit GCTGAAGCTCCTGAGATCAGAAGGCTTGGAAAGCATCATTCATATGATAA 5 0.125 No Hit TGTTGGTGGAGGCTGAATTTCCAGAGGATATAATAAATGCCCGCCTGGAA 5 0.125 No Hit CTTCAATCTTCTCTGATCAGATCCCTCAAAGCTATAAAAATGATGAAAGC 5 0.125 No Hit TGCTTAATTACCTCTCTCTCTCTCTAGCTTGTAATCGGATCCTTACTAGT 5 0.125 No Hit CAAGAGTGGTTCACGCAAAAGGACAGCGAGAGAAAAGTAAAGGCAAGAGT 5 0.125 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.025 0.0 0.0 0.0 0.0 44-45 0.025 0.0 0.0 0.0 0.0 46-47 0.025 0.0 0.0 0.0 0.0 48-49 0.025 0.0 0.0 0.0 0.0 50-51 0.025 0.0 0.0 0.0 0.0 52-53 0.025 0.0 0.0 0.0 0.0 54-55 0.025 0.0 0.0 0.0 0.0 56-57 0.025 0.0 0.0 0.0 0.0 58-59 0.025 0.0 0.0 0.0 0.0 60-61 0.025 0.0 0.0 0.0 0.0 62-63 0.025 0.0 0.0 0.0 0.0 64-65 0.025 0.0 0.0 0.0 0.0 66-67 0.025 0.0 0.0 0.0 0.0 68-69 0.025 0.0 0.0 0.0 0.0 70-71 0.0625 0.0 0.0 0.0 0.0 72-73 0.125 0.0 0.0 0.0 0.0 74-75 0.1375 0.0 0.0 0.0 0.0 76-77 0.1875 0.0 0.0 0.0 0.0 78-79 0.25 0.0 0.0 0.0 0.0 80-81 0.275 0.0 0.0 0.0 0.0 82-83 0.3375 0.0 0.0 0.0 0.0 84-85 0.48750000000000004 0.0 0.0 0.0 0.0 86-87 0.6 0.0 0.0 0.0 0.0 88-89 0.65 0.0 0.0 0.0 0.0 90-91 0.7250000000000001 0.0 0.0 0.0 0.0 92-93 0.9875 0.0 0.0 0.0 0.0 94-95 1.375 0.0 0.0 0.0 0.0 96-97 1.6625 0.0 0.0 0.0 0.0 98-99 1.875 0.0 0.0 0.0 0.0 100-101 2.1125 0.0 0.0 0.0 0.0 102-103 2.45 0.0 0.0 0.0 0.0 104-105 2.9000000000000004 0.0 0.0 0.0 0.0 106-107 3.4125 0.0 0.0 0.0 0.0 108-109 4.0375 0.0 0.0 0.0 0.0 110-111 4.5125 0.0 0.0 0.0 0.0 112-113 5.0125 0.0 0.0 0.0 0.0 114-115 5.6375 0.0 0.0 0.0 0.0 116-117 6.15 0.0 0.0 0.0 0.0 118-119 6.8 0.0 0.0 0.0 0.0 120-121 7.65 0.0 0.0 0.0 0.0 122-123 8.425 0.0 0.0 0.0 0.0 124-125 9.212499999999999 0.0 0.0 0.0 0.0 126-127 10.2375 0.0 0.0 0.0 0.0 128-129 10.912500000000001 0.0 0.0 0.0 0.0 130-131 11.7 0.0 0.0 0.0 0.0 132-133 12.3625 0.0 0.0 0.0 0.0 134-135 13.2125 0.0 0.0 0.0 0.0 136-137 14.3125 0.0 0.0 0.0 0.0 138-139 15.25 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ACCACCC 10 0.006830828 145.0 2 CCTTGAC 10 0.006830828 145.0 7 CCACCCT 10 0.006830828 145.0 3 TTGACGA 10 0.006830828 145.0 9 ATGTTGT 10 0.006830828 145.0 145 CTTGACG 10 0.006830828 145.0 8 CCCTTGA 10 0.006830828 145.0 6 >>END_MODULE Read 1480341 spots for SRR26075362.sra Written 1480341 spots for SRR26075362.sra Read 1480341 spots for SRR26075362.sra Written 1480341 spots for SRR26075362.sra Read 1480341 spots for SRR26075362.sra Written 1480341 spots for SRR26075362.sra Read 1480341 spots for SRR26075362.sra Written 1480341 spots for SRR26075362.sra Read 1480341 spots for SRR26075362.sra Written 1480341 spots for SRR26075362.sra Read 1480341 spots for SRR26075362.sra Written 1480341 spots for SRR26075362.sra Read 1480341 spots for SRR26075362.sra Written 1480341 spots for SRR26075362.sra Read 1480341 spots for SRR26075362.sra Written 1480341 spots for SRR26075362.sra Read 1480341 spots for SRR26075362.sra Written 1480341 spots for SRR26075362.sra Read 1480358 spots for SRR26075362.sra Written 1480358 spots for SRR26075362.sra Read 1480341 spots for SRR26075362.sra Written 1480341 spots for SRR26075362.sra Read 1480341 spots for SRR26075362.sra Written 1480341 spots for SRR26075362.sra Read 1480341 spots for SRR26075362.sra Written 1480341 spots for SRR26075362.sra Read 1480341 spots for SRR26075362.sra Written 1480341 spots for SRR26075362.sra Read 1480341 spots for SRR26075362.sra Written 1480341 spots for SRR26075362.sra Read 1480341 spots for SRR26075362.sra Written 1480341 spots for SRR26075362.sra Read 1480341 spots for SRR26075362.sra Written 1480341 spots for SRR26075362.sra Read 1480341 spots for SRR26075362.sra Written 1480341 spots for SRR26075362.sra Read 1480341 spots for SRR26075362.sra Written 1480341 spots for SRR26075362.sra Read 1480341 spots for SRR26075362.sra Written 1480341 spots for SRR26075362.sra SRR ids: ['SRR26075362.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_k74yaum9 SRR26075362.sra spots: 29606837 blocks: [[1, 1480341], [1480342, 2960682], [2960683, 4441023], [4441024, 5921364], [5921365, 7401705], [7401706, 8882046], [8882047, 10362387], [10362388, 11842728], [11842729, 13323069], [13323070, 14803410], [14803411, 16283751], [16283752, 17764092], [17764093, 19244433], [19244434, 20724774], [20724775, 22205115], [22205116, 23685456], [23685457, 25165797], [25165798, 26646138], [26646139, 28126479], [28126480, 29606837]] SRR26075362 file size 10931677 SRR26075362 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o SRR26075362 SRR26075362_1.fastq SRR26075362_2.fastq Input file: SRR26075362_1.fastq Paired file: SRR26075362_2.fastq trimmed: SRR26075362-trimmed-pair1.fastq, SRR26075362-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Tue Feb 11 22:18:15 2025 >> started Tue Feb 11 22:18:50 2025 >> done (34.210s) 29606837 read pairs processed; of these: 125 ( 0.00%) short read pairs filtered out after trimming by size control 229312 ( 0.77%) empty read pairs filtered out after trimming by size control 29377400 (99.23%) read pairs available; of these: 6151963 (20.94%) trimmed read pairs available after processing 23225437 (79.06%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 18 19 0.00% 19 13 0.00% 20 12 0.00% 21 16 0.00% 22 15 0.00% 23 22 0.00% 24 29 0.00% 25 29 0.00% 26 34 0.00% 27 36 0.00% 28 36 0.00% 29 19 0.00% 30 45 0.00% 31 42 0.00% 32 40 0.00% 33 55 0.00% 34 45 0.00% 35 46 0.00% 36 72 0.00% 37 72 0.00% 38 70 0.00% 39 89 0.00% 40 92 0.00% 41 97 0.00% 42 110 0.00% 43 123 0.00% 44 114 0.00% 45 90 0.00% 46 127 0.00% 47 132 0.00% 48 219 0.00% 49 198 0.00% 50 280 0.00% 51 299 0.00% 52 373 0.00% 53 342 0.00% 54 343 0.00% 55 400 0.00% 56 436 0.00% 57 537 0.00% 58 693 0.00% 59 718 0.00% 60 839 0.00% 61 1004 0.00% 62 1126 0.00% 63 1323 0.00% 64 1480 0.01% 65 1571 0.01% 66 1620 0.01% 67 2184 0.01% 68 2239 0.01% 69 2747 0.01% 70 3034 0.01% 71 3651 0.01% 72 4156 0.01% 73 4827 0.02% 74 5417 0.02% 75 6255 0.02% 76 6743 0.02% 77 7567 0.03% 78 8322 0.03% 79 9499 0.03% 80 10275 0.03% 81 11714 0.04% 82 13668 0.05% 83 14997 0.05% 84 17260 0.06% 85 18732 0.06% 86 19990 0.07% 87 21357 0.07% 88 23178 0.08% 89 24857 0.08% 90 27648 0.09% 91 29839 0.10% 92 31979 0.11% 93 34866 0.12% 94 37394 0.13% 95 40554 0.14% 96 42888 0.15% 97 45273 0.15% 98 46739 0.16% 99 48991 0.17% 100 50243 0.17% 101 52530 0.18% 102 55844 0.19% 103 59166 0.20% 104 62666 0.21% 105 65900 0.22% 106 69311 0.24% 107 70563 0.24% 108 72123 0.25% 109 74383 0.25% 110 76466 0.26% 111 78565 0.27% 112 82432 0.28% 113 83010 0.28% 114 86558 0.29% 115 90969 0.31% 116 93158 0.32% 117 95905 0.33% 118 98298 0.33% 119 100193 0.34% 120 100423 0.34% 121 101624 0.35% 122 103963 0.35% 123 106944 0.36% 124 109333 0.37% 125 113489 0.39% 126 115480 0.39% 127 118040 0.40% 128 119592 0.41% 129 120959 0.41% 130 121761 0.41% 131 121411 0.41% 132 125278 0.43% 133 125784 0.43% 134 127777 0.43% 135 130891 0.45% 136 133086 0.45% 137 134167 0.46% 138 135471 0.46% 139 138011 0.47% 140 138422 0.47% 141 139478 0.47% 142 139200 0.47% 143 140824 0.48% 144 143161 0.49% 145 144277 0.49% 146 145477 0.50% 147 147009 0.50% 148 149371 0.51% 149 147876 0.50% 150 150719 0.51% 151 23225437 79.06% 29377400 reads passed initial QC criterion=sequence-density sequence-density=0.31 sequence-density-rank=1 fanout-score=2.66 fanout-score-rank=35 prefix-density=0.32 prefix-fanout=2.5 sequence=GTGGACTCCTTCTGGAT criterion=fanout-score sequence-density=0.01 sequence-density-rank=41 fanout-score=49.10 fanout-score-rank=1 prefix-density=0.14 prefix-fanout=2.8 sequence=TCAAATAAAGCCACACTAGTATTCATTATTGATGTCTGTGATCAAATAACAAAGAGCGTGACGCGACCAAACCCATAGCCACCACCATCTAGTAACAGAACCATATCCTGCA criterion=sequence-density sequence-density=0.29 sequence-density-rank=1 fanout-score=2.83 fanout-score-rank=33 prefix-density=0.35 prefix-fanout=2.4 sequence=ATCCAGAAGGAGTCCACCCTCCACTTGGTGCTTCG criterion=fanout-score sequence-density=0.15 sequence-density-rank=10 fanout-score=74.50 fanout-score-rank=1 prefix-density=0.69 prefix-fanout=16.2 sequence=GATGATGATGCT SRR26075362 testing PE reads STAR mapping to Ensembl genome Started job on | Feb 11 22:19:28 Started mapping on | Feb 11 22:19:28 Finished on | Feb 11 22:23:13 Mapping speed, Million of reads per hour | 470.04 Number of input reads | 29377400 Average input read length | 291 UNIQUE READS: Uniquely mapped reads number | 26483292 Uniquely mapped reads % | 90.15% Average mapped length | 289.23 Number of splices: Total | 20208495 Number of splices: Annotated (sjdb) | 19668411 Number of splices: GT/AG | 19863383 Number of splices: GC/AG | 257707 Number of splices: AT/AC | 19920 Number of splices: Non-canonical | 67485 Mismatch rate per base, % | 0.43% Deletion rate per base | 0.03% Deletion average length | 2.92 Insertion rate per base | 0.02% Insertion average length | 2.61 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 882084 % of reads mapped to multiple loci | 3.00% Number of reads mapped to too many loci | 105862 % of reads mapped to too many loci | 0.36% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 5.97% % of reads unmapped: other | 0.52% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 2012024 2012024 2012024 N_multimapping 882084 882084 882084 N_noFeature 739445 26112869 972744 N_ambiguous 282608 2310 144063 UnstrandedReadsAssigned:25461239 PositiveStrandReadsAssigned:368113 NegativeStrandReadsAssigned:25366485 Dataset is classified negative stranded MeadianReadLen=151 20thPercentileLength=147 echo kmer=143 SRR26075362 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,400 [index] number of k-mers: 62,057,036 [index] number of equivalence classes: 130,681 [quant] running in paired-end mode [quant] will process pair 1: SRR26075362-trimmed-pair1.fastq SRR26075362-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 29,377,400 reads, 26,027,122 reads pseudoaligned [quant] estimated average fragment length: 200.479 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,177 rounds 52401 SRR26075362.ke.tsv 34699 SRR26075362.se.tsv 87100 total ==> SRR26075362.ke.tsv <== target_id length eff_length est_counts tpm Potri.005G200100.1.v4.1 2018 1818.52 4084 68.4241 Potri.005G024800.1.v4.1 1035 835.521 13272.3 483.985 Potri.004G059700.1.v4.1 961 761.533 28 1.12024 Potri.007G009000.2.v4.1 1416 1216.52 0 0 Potri.003G141000.2.v4.1 2943 2743.52 1011 11.2275 Potri.016G087400.1.v4.1 270 97.0152 2283.08 717.007 Potri.015G069301.1.v4.1 564 365.541 0 0 Potri.010G195200.1.v4.1 1773 1573.52 94 1.82011 Potri.012G127500.1.v4.1 977 777.533 7142 279.861 ==> SRR26075362.se.tsv <== Potri.001G166300.v4.1 0 Potri.001G448400.v4.1 31 Potri.001G233950.v4.1 0 Potri.001G122700.v4.1 221 Potri.001G212900.v4.1 5 Potri.001G182400.v4.1 0 Potri.001G256600.v4.1 0 Potri.001G040500.v4.1 9 Potri.001G416900.v4.1 0 Potri.001G452600.v4.1 675 SRR26075362 completed mapping pipeline successfully