Starting /dee2/code/volunteer_pipeline.sh SRR26075363 current disk space = 3052204060672 free memory = 1514868004 SRR26075363 SRAfilesize 4f7ac50305c7a7538c6e955ab76c1e54 SRR26075363.sra SRR26075363.sra file validated SRR26075363 is paired end SRR26075363 is conventional basespace SRR26075363 read1 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR26075363_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.6755 37.0 37.0 37.0 37.0 37.0 2 36.6185 37.0 37.0 37.0 37.0 37.0 3 36.607 37.0 37.0 37.0 37.0 37.0 4 36.642 37.0 37.0 37.0 37.0 37.0 5 36.669 37.0 37.0 37.0 37.0 37.0 6 36.7305 37.0 37.0 37.0 37.0 37.0 7 36.6715 37.0 37.0 37.0 37.0 37.0 8 36.7255 37.0 37.0 37.0 37.0 37.0 9 36.729 37.0 37.0 37.0 37.0 37.0 10-14 36.65195 37.0 37.0 37.0 37.0 37.0 15-19 36.5792 37.0 37.0 37.0 37.0 37.0 20-24 36.563 37.0 37.0 37.0 37.0 37.0 25-29 36.5017 37.0 37.0 37.0 37.0 37.0 30-34 36.4664 37.0 37.0 37.0 37.0 37.0 35-39 36.4676 37.0 37.0 37.0 37.0 37.0 40-44 36.326499999999996 37.0 37.0 37.0 37.0 37.0 45-49 36.25849999999999 37.0 37.0 37.0 37.0 37.0 50-54 36.2592 37.0 37.0 37.0 37.0 37.0 55-59 36.076 37.0 37.0 37.0 37.0 37.0 60-64 35.990100000000005 37.0 37.0 37.0 37.0 37.0 65-69 35.9013 37.0 37.0 37.0 37.0 37.0 70-74 35.9735 37.0 37.0 37.0 37.0 37.0 75-79 35.947199999999995 37.0 37.0 37.0 37.0 37.0 80-84 35.9768 37.0 37.0 37.0 37.0 37.0 85-89 35.847699999999996 37.0 37.0 37.0 37.0 37.0 90-94 35.8774 37.0 37.0 37.0 37.0 37.0 95-99 35.867000000000004 37.0 37.0 37.0 37.0 37.0 100-104 35.822900000000004 37.0 37.0 37.0 37.0 37.0 105-109 35.7539 37.0 37.0 37.0 37.0 37.0 110-114 35.5493 37.0 37.0 37.0 37.0 37.0 115-119 35.5332 37.0 37.0 37.0 37.0 37.0 120-124 35.4899 37.0 37.0 37.0 37.0 37.0 125-129 35.305899999999994 37.0 37.0 37.0 34.6 37.0 130-134 35.1868 37.0 37.0 37.0 29.8 37.0 135-139 35.1245 37.0 37.0 37.0 29.8 37.0 140-144 34.977000000000004 37.0 37.0 37.0 25.0 37.0 145-149 34.924600000000005 37.0 37.0 37.0 25.0 37.0 150-151 34.79975 37.0 37.0 37.0 25.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.0 1101 2 0.0 1101 3 0.0 1101 4 0.0 1101 5 0.0 1101 6 0.0 1101 7 0.0 1101 8 0.0 1101 9 0.0 1101 10-14 0.0 1101 15-19 0.0 1101 20-24 0.0 1101 25-29 0.0 1101 30-34 0.0 1101 35-39 0.0 1101 40-44 0.0 1101 45-49 0.0 1101 50-54 0.0 1101 55-59 0.0 1101 60-64 0.0 1101 65-69 0.0 1101 70-74 0.0 1101 75-79 0.0 1101 80-84 0.0 1101 85-89 0.0 1101 90-94 0.0 1101 95-99 0.0 1101 100-104 0.0 1101 105-109 0.0 1101 110-114 0.0 1101 115-119 0.0 1101 120-124 0.0 1101 125-129 0.0 1101 130-134 0.0 1101 135-139 0.0 1101 140-144 0.0 1101 145-149 0.0 1101 150-151 0.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 20 2.0 21 2.0 22 4.0 23 7.0 24 9.0 25 14.0 26 13.0 27 13.0 28 19.0 29 19.0 30 27.0 31 56.0 32 47.0 33 104.0 34 185.0 35 447.0 36 2817.0 37 215.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 35.11011011011011 16.916916916916914 8.583583583583582 39.38938938938939 2 19.725 15.325 35.4 29.549999999999997 3 18.075 16.525000000000002 28.425 36.975 4 21.925 22.15 24.525 31.4 5 24.55 24.5 25.45 25.5 6 23.35 31.924999999999997 23.375 21.349999999999998 7 14.35 30.25 37.6 17.8 8 17.275 28.000000000000004 33.725 21.0 9 18.65 23.925 34.4 23.025000000000002 10-14 19.34596729836492 29.651482574128707 28.046402320116005 22.95614780739037 15-19 20.03 27.6 28.37 24.0 20-24 20.044999999999998 27.99 28.754999999999995 23.21 25-29 19.59 27.855 28.205000000000002 24.349999999999998 30-34 20.16 28.735 27.145000000000003 23.96 35-39 20.65 27.034999999999997 29.13 23.185 40-44 19.81 28.08 27.415 24.695 45-49 20.7 27.700000000000003 28.12 23.48 50-54 20.05 27.61 27.975 24.365000000000002 55-59 20.330000000000002 28.444999999999997 26.855 24.37 60-64 20.415 27.975 27.93 23.68 65-69 20.68 27.465 27.815 24.04 70-74 21.945 28.470000000000002 26.85 22.735 75-79 20.515 27.775 27.565 24.145 80-84 22.189999999999998 27.139999999999997 27.794999999999998 22.875 85-89 21.325 28.15 27.169999999999998 23.355 90-94 21.075 28.465 26.77 23.69 95-99 21.61 28.249999999999996 26.889999999999997 23.25 100-104 21.445 26.945000000000004 27.279999999999998 24.33 105-109 21.634999999999998 27.375 26.685 24.305 110-114 22.195 27.534999999999997 27.27 23.0 115-119 21.685 27.825 27.060000000000002 23.43 120-124 22.375 26.745 27.205000000000002 23.674999999999997 125-129 21.95 27.92 25.900000000000002 24.23 130-134 22.21 27.455000000000002 26.465 23.87 135-139 22.045 27.169999999999998 26.775 24.01 140-144 22.09 27.525 26.625 23.76 145-149 22.0 26.745 26.58 24.675 150-151 22.912499999999998 27.0875 25.1 24.9 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.5 12 0.5 13 0.5 14 1.5 15 1.5 16 0.5 17 0.5 18 1.0 19 1.5 20 1.5 21 0.5 22 0.5 23 2.5 24 2.5 25 2.5 26 6.0 27 8.0 28 8.5 29 11.5 30 17.0 31 25.5 32 29.5 33 45.0 34 63.5 35 68.5 36 81.0 37 100.0 38 130.5 39 154.0 40 190.0 41 218.0 42 217.5 43 234.0 44 249.5 45 257.0 46 234.5 47 201.0 48 220.5 49 234.5 50 181.0 51 133.0 52 127.0 53 120.0 54 92.0 55 59.5 56 47.5 57 38.5 58 29.5 59 27.0 60 20.0 61 15.0 62 10.0 63 11.5 64 13.5 65 9.0 66 9.5 67 9.0 68 5.5 69 5.5 70 4.0 71 2.5 72 3.5 73 1.5 74 0.5 75 1.0 76 0.5 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.1 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.005 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150-151 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 59.85 #Duplication Level Percentage of deduplicated Percentage of total 1 62.531328320802004 37.425000000000004 2 21.84628237259816 26.150000000000002 3 8.771929824561402 15.75 4 3.8011695906432745 9.1 5 1.3784461152882206 4.125 6 0.835421888053467 3.0 7 0.29239766081871343 1.225 8 0.3341687552213868 1.6 9 0.0835421888053467 0.44999999999999996 >10 0.12531328320802004 1.175 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCGTCTATCTCGTAT 27 0.675 TruSeq Adapter, Index 10 (97% over 39bp) CCATCCTTCACAAGCTTCCTTATGTTCTGGCGTGAATTAGCCATGGAGAT 10 0.25 No Hit CCCAAAACCGAGATTATATCCTGTGTTGCCGCAATCTTTAATTACTGGTT 10 0.25 No Hit CCTTGGAATTATGGCACAATTTATCTCTTCCCCATATTTTTCATCCGGAA 9 0.22499999999999998 No Hit GACGCGTCCTGTTGGGCCTCCACTCGATGTCTAGGCCAACTAGAAGGGGA 9 0.22499999999999998 No Hit AGTAGTAGTGTGGATGTTGTTGTTAGACACTTCTTTTTGTCTTTAAATAT 8 0.2 No Hit CACTGCACTCAATTTCTTCACTTCGTTTTACTGCCACAAGAAACTTTTGG 8 0.2 No Hit GTGCGAATCAACGGTTCCTCTCGTACTAGGTTGGATTACTATTGCGACAC 8 0.2 No Hit CACTGCACTACTTCCCTTGGAGACTTTCCCTGTAGTGCTCTTCTCCTTCT 8 0.2 No Hit GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCGTCTATCGCGTAT 8 0.2 TruSeq Adapter, Index 10 (97% over 39bp) GCTTGTTTTTAAACGATAACAAATCCAATTATGCATATACAACCAAGATT 8 0.2 No Hit ACCCACCCAATTCTTCAACCCGCCTTCGCTCTGATGGATAAATAGGCCTA 8 0.2 No Hit ATCCTCATGTCGCAGCAAATGTTGACACTCAACTTTACCAGGACCGCCCA 8 0.2 No Hit CCAGGAATTACTGACCATAGTGCTCGTACGCTAGTCTAGCCTAGTAAAAC 7 0.17500000000000002 No Hit TGGTGCAGTTTGATCCACACTTGCAGCCATTCTCAGCACCGAAGTCCATC 7 0.17500000000000002 No Hit CAGGGAAGAAGGACGCAACTGACCAAGAGATGGACCTCCATCCTTCTGAA 7 0.17500000000000002 No Hit GTCGTGAGTTCAGCACAACATCGTATGATAATACAAACATATGATATTTT 7 0.17500000000000002 No Hit GGCACATAGATTAGGACGAAGATCAAAGCCACAACAGATATGATCCCAAA 7 0.17500000000000002 No Hit ACTGCTCAACATCTGGACCAGTAACTCTAGTGGTGAAGGGATCAACTCTC 7 0.17500000000000002 No Hit ATCACAATATATATTACACGCGTTAATTAACCTAGCTTACTACTAGATCT 7 0.17500000000000002 No Hit GGCATGTAGCCTTGTTATTTACTGAGCCTAGCAAGCACAGAAAGTTGGCT 6 0.15 No Hit AGAGTAGCCTCTGAGTTAGCTTTGCACCTGCAAAGGAATGCTGCACGAGC 6 0.15 No Hit TTTTCCTAAATATTCTGTACCTCCTTTTTCTTTGCTCTTATCTACAATCC 6 0.15 No Hit GGAACAACAGAATCATATTTCTGGAAGCAATCCTCTGGCCGACGATTCAT 6 0.15 No Hit AGCTCCTTGTAGTCCTTGTCATCTAGGCTCTGAGCTGCCGTGCCAATTGG 6 0.15 No Hit ATTCTGACCATACATTGGGAATACTCGCCCCAGTAGTTTCTGTTGCCTTA 6 0.15 No Hit CCTCTCTGAGCTTGAGCCTTTTTTCTGCACTTTGCCATTGGCAGCGGGGC 6 0.15 No Hit CATTGACGGACTCAGAGTACCACGCTTGGAGCTCTTTGTCATTACAGACT 6 0.15 No Hit CTCCTCTTTGAACCTCCACGTGAGTATACGATGCCTCTTTTCTGGAGCTT 6 0.15 No Hit CCTCCACAGCCGCTGCCGCACTTGCAGCCAGAGCCACACCCACAGTTTCC 6 0.15 No Hit CTTAGTAGTCAAACTATCTGCAGTGACAGCATCCTTCAAATCCACATCCA 6 0.15 No Hit CCTTGTCAGTGAAAACTCCAGTTGACTCCACAATATACTCAGCTCCGGTC 6 0.15 No Hit GCAATCCAGACCTTCTTGTGCATCTTTCCTCGAATATGGCAAAGACGCTT 6 0.15 No Hit ATCACCATAGATATAGAGCTGAAACCTCCATTACTGGTCCCTTTCTGTCA 6 0.15 No Hit GGTGCTTCATTTGGAGAGAACATGTTAATTGTCTCATACTCTACAGTCCC 6 0.15 No Hit CTCTCCCTCACATGCTACAATGTTTACTATGTCCCGTGCCAGACACTGTT 6 0.15 No Hit TGCAAATTTCAATCCTGGGGTCAGCAGCAAGTGCCTTGTTGAGCTCCAAA 6 0.15 No Hit CTAGCACTTATCCCAATCATGGGAAAATGGTTCCCAAGCTGAAAGTATAC 6 0.15 No Hit GGAGCAAGAAGGGTCCATGTGTAGAGTAAACCAGTGATCCATTCTGTGCA 6 0.15 No Hit CCCATATGCAGTGGCCTCCAGCCCACTGGCACACTCTAAGCAAGCACCAG 6 0.15 No Hit GGGGCTTGTAGCTCAGTGGCCTTGGCGGGCTTTGCACTGCCTAAGTGCCC 5 0.125 No Hit ATCGAGGAGATTCATCTTCTCATCACTCACAAGCAAGTCGTGGCGTAGGA 5 0.125 No Hit ATTGGACCTAGCCCATCAATGGATCCCTTCTTGGGGTCGACAATGGTGGC 5 0.125 No Hit CCTTTCTTCACCTTCTTAATGAACACAATGCATGAAAGATACATATACTC 5 0.125 No Hit GCAACAATAACTGAAAACTACAATGAAACGACACAAGAAGTTCGCTCCCT 5 0.125 No Hit CATTGAAGAAGCTTCTTTCCACTGTTTCTAGAACCTTCGCCAAGCAAAGG 5 0.125 No Hit GGGAGCTTGATTGATAATTCTGTATAAGGTGATCGCAGGTTGTGCAATCA 5 0.125 No Hit CAGTATTTTATACAATGTTTTCAAGATCGGGCAGAGTTCAACAGGTGCAA 5 0.125 No Hit CAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAACCAATG 5 0.125 No Hit CGACAAACTTCGATCTTGGAGGGATACTGGTACAAAAAGTGCAAGTATAT 5 0.125 No Hit CAAGTCATTACAACCAATAAAGAGAGATAAGATGTTTCCCCATAAAATGG 5 0.125 No Hit AGAGGGCTCTGATTCAACTGCTGATTCCAAAGAGCCGGAAAAGTAGGACC 5 0.125 No Hit GGCACTATGGTAGGATGGCTAGTGTGCAAGTAATTCATTCCCTTTGCCAC 5 0.125 No Hit GTCTCGGGTTCAGAAACCTGAAGTTTCTCTTCAAATTCAGTACCAATCAC 5 0.125 No Hit ATCATATTTTCTCCCTAAAATCTCAATCTGTGCCGTCTGATTGTCTCGTC 5 0.125 No Hit CCTAACTGGTTTGCTGCTATCACAGAGATATAATAAGATCAAACCGATAC 5 0.125 No Hit CCTCATACATGTTGCAAAGATTATCTACACTCATTGCTTGCTTCAGCAGA 5 0.125 No Hit CTTCAGATGTACCCCTCAAAGAAATAGGTTTCTTGTATTGTGTTCACATG 5 0.125 No Hit GCCTATTTGCATTACAGACACACCTCTATACGAACCATAGACATTTCTTC 5 0.125 No Hit GTGACAAAAGGCTGCTGGCAATTCGCAAATAAAATAAAAGGAAAGCAAAA 5 0.125 No Hit CTCTCGTTCATAAGCTCACCCAGCTTCTTTAGCTTGTCCTCATCACTTAA 5 0.125 No Hit CCGGGAGCTTGTCCTTCATCTGGTCAACAAACCCTTCCTTGCGCTCACCT 5 0.125 No Hit GTGGTTTCCACAATCAGCAATGAGCACATTGTAAGGTATGTTGTTTTCTT 5 0.125 No Hit GTTTGGGAACATGGAAGGACTGGCTCCTCCACACTTTGTAGAACTTCTCT 5 0.125 No Hit GGGTACCTAACGGTTGGCTTTGTACCATCATTGTTACAGTTATCGTATTT 5 0.125 No Hit GGCGTTGTAGGAGGCTAAGTAGCTAACTCCTGAGTCTGAACTTGTTTTAC 5 0.125 No Hit CTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGCTG 5 0.125 No Hit CCCTTGACTCCAGCCATCAAAGGAGCCCATTACTTGTACACTCTCTGCCA 5 0.125 No Hit ACCCTAACGACACTTAAAGATTCAAATGGAAAGTCTTCAAAATACAGCAT 5 0.125 No Hit CGTCTATCCAGTGACTCATGAACGCTTTCCACCTGTTGAGAGATGGCATC 5 0.125 No Hit CTTTAAGGGCGGGAATTTGGTACCGTGTGGGGGAGGTTCCCAAATTGGTT 5 0.125 No Hit AACGCATTCCTACTTTCCTAGTCTCATATATAGGCAACAACAAACACAAG 5 0.125 No Hit CTCCTCATTATGGCCTCCTGCTCTTCAGTGACAGCTTGAAGAACACTTGG 5 0.125 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.025 0.0 0.0 0.0 0.0 64-65 0.07500000000000001 0.0 0.0 0.0 0.0 66-67 0.1 0.0 0.0 0.0 0.0 68-69 0.1 0.0 0.0 0.0 0.0 70-71 0.16249999999999998 0.0 0.0 0.0 0.0 72-73 0.2 0.0 0.0 0.0 0.0 74-75 0.21250000000000002 0.0 0.0 0.0 0.0 76-77 0.25 0.0 0.0 0.0 0.0 78-79 0.2625 0.0 0.0 0.0 0.0 80-81 0.4 0.0 0.0 0.0 0.0 82-83 0.7125 0.0 0.0 0.0 0.0 84-85 0.9 0.0 0.0 0.0 0.0 86-87 1.075 0.0 0.0 0.0 0.0 88-89 1.1875 0.0 0.0 0.0 0.0 90-91 1.4 0.0 0.0 0.0 0.0 92-93 1.6749999999999998 0.0 0.0 0.0 0.0 94-95 1.875 0.0 0.0 0.0 0.0 96-97 2.475 0.0 0.0 0.0 0.0 98-99 2.9000000000000004 0.0 0.0 0.0 0.0 100-101 3.2625 0.0 0.0 0.0 0.0 102-103 3.5 0.0 0.0 0.0 0.0 104-105 3.825 0.0 0.0 0.0 0.0 106-107 4.25 0.0 0.0 0.0 0.0 108-109 4.8125 0.0 0.0 0.0 0.0 110-111 5.25 0.0 0.0 0.0 0.0 112-113 5.725 0.0 0.0 0.0 0.0 114-115 6.449999999999999 0.0 0.0 0.0 0.0 116-117 7.0875 0.0 0.0 0.0 0.0 118-119 7.875 0.0 0.0 0.0 0.0 120-121 8.925 0.0 0.0 0.0 0.0 122-123 9.825 0.0 0.0 0.0 0.0 124-125 10.825 0.0 0.0 0.0 0.0 126-127 11.3625 0.0 0.0 0.0 0.0 128-129 12.55 0.0 0.0 0.0 0.0 130-131 13.4375 0.0 0.0 0.0 0.0 132-133 14.0 0.0 0.0 0.0 0.0 134-135 14.9 0.0 0.0 0.0 0.0 136-137 15.7375 0.0 0.0 0.0 0.0 138-139 16.3 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GAGCACA 65 0.0076375785 22.307692 9 ACTCCAG 55 0.0025160722 15.818182 140-144 CTGAACT 60 0.004491891 14.500001 135-139 TCTGAAC 60 0.004491891 14.500001 135-139 AGCACAC 65 0.0076375785 13.384615 125-129 >>END_MODULE SRR26075363 read2 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR26075363_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.28425 37.0 37.0 37.0 37.0 37.0 2 36.38 37.0 37.0 37.0 37.0 37.0 3 36.3455 37.0 37.0 37.0 37.0 37.0 4 36.428 37.0 37.0 37.0 37.0 37.0 5 36.4165 37.0 37.0 37.0 37.0 37.0 6 36.199 37.0 37.0 37.0 37.0 37.0 7 36.229 37.0 37.0 37.0 37.0 37.0 8 36.395 37.0 37.0 37.0 37.0 37.0 9 36.307 37.0 37.0 37.0 37.0 37.0 10-14 36.2613 37.0 37.0 37.0 37.0 37.0 15-19 36.1859 37.0 37.0 37.0 37.0 37.0 20-24 36.1952 37.0 37.0 37.0 37.0 37.0 25-29 36.021 37.0 37.0 37.0 37.0 37.0 30-34 35.955499999999994 37.0 37.0 37.0 37.0 37.0 35-39 35.8542 37.0 37.0 37.0 37.0 37.0 40-44 35.813700000000004 37.0 37.0 37.0 37.0 37.0 45-49 35.7478 37.0 37.0 37.0 37.0 37.0 50-54 35.60680000000001 37.0 37.0 37.0 37.0 37.0 55-59 35.6216 37.0 37.0 37.0 37.0 37.0 60-64 35.6858 37.0 37.0 37.0 37.0 37.0 65-69 35.630700000000004 37.0 37.0 37.0 37.0 37.0 70-74 35.5166 37.0 37.0 37.0 37.0 37.0 75-79 35.468900000000005 37.0 37.0 37.0 37.0 37.0 80-84 35.5402 37.0 37.0 37.0 37.0 37.0 85-89 35.547000000000004 37.0 37.0 37.0 37.0 37.0 90-94 35.39 37.0 37.0 37.0 37.0 37.0 95-99 35.547700000000006 37.0 37.0 37.0 37.0 37.0 100-104 35.396899999999995 37.0 37.0 37.0 37.0 37.0 105-109 35.45819999999999 37.0 37.0 37.0 37.0 37.0 110-114 35.3584 37.0 37.0 37.0 37.0 37.0 115-119 35.2768 37.0 37.0 37.0 34.6 37.0 120-124 35.17055 37.0 37.0 37.0 29.8 37.0 125-129 35.251999999999995 37.0 37.0 37.0 37.0 37.0 130-134 35.126799999999996 37.0 37.0 37.0 27.4 37.0 135-139 34.969100000000005 37.0 37.0 37.0 25.0 37.0 140-144 34.9695 37.0 37.0 37.0 25.0 37.0 145-149 34.870400000000004 37.0 37.0 37.0 25.0 37.0 150-151 34.69625 37.0 37.0 37.0 25.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.0 1101 2 0.0 1101 3 0.0 1101 4 0.0 1101 5 0.0 1101 6 0.0 1101 7 0.0 1101 8 0.0 1101 9 0.0 1101 10-14 0.0 1101 15-19 0.0 1101 20-24 0.0 1101 25-29 0.0 1101 30-34 0.0 1101 35-39 0.0 1101 40-44 0.0 1101 45-49 0.0 1101 50-54 0.0 1101 55-59 0.0 1101 60-64 0.0 1101 65-69 0.0 1101 70-74 0.0 1101 75-79 0.0 1101 80-84 0.0 1101 85-89 0.0 1101 90-94 0.0 1101 95-99 0.0 1101 100-104 0.0 1101 105-109 0.0 1101 110-114 0.0 1101 115-119 0.0 1101 120-124 0.0 1101 125-129 0.0 1101 130-134 0.0 1101 135-139 0.0 1101 140-144 0.0 1101 145-149 0.0 1101 150-151 0.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 12 2.0 13 3.0 14 7.0 15 3.0 16 11.0 17 5.0 18 6.0 19 4.0 20 5.0 21 7.0 22 9.0 23 6.0 24 8.0 25 16.0 26 10.0 27 21.0 28 20.0 29 20.0 30 24.0 31 44.0 32 52.0 33 83.0 34 202.0 35 663.0 36 2552.0 37 217.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 41.08527131782946 21.45536384096024 13.553388347086774 23.905976494123532 2 28.999999999999996 26.724999999999998 27.0 17.275 3 22.3 30.675 28.95 18.075 4 26.25 32.7 22.15 18.9 5 28.65 35.975 19.15 16.225 6 22.325 38.574999999999996 21.975 17.125 7 23.0 21.85 35.825 19.325 8 24.7 26.5 26.25 22.55 9 26.025 26.075 27.250000000000004 20.65 10-14 25.275 29.459999999999997 24.945 20.32 15-19 25.215 28.68 25.695 20.41 20-24 25.095 28.860000000000003 25.069999999999997 20.974999999999998 25-29 25.985000000000003 27.565 25.75 20.7 30-34 25.8 27.950000000000003 25.45 20.8 35-39 25.324999999999996 27.96 25.805 20.91 40-44 25.485000000000003 26.945000000000004 27.045 20.525 45-49 24.865000000000002 28.87 26.265 20.0 50-54 23.515 27.905 27.345000000000002 21.235 55-59 24.6 28.435 26.76 20.205000000000002 60-64 25.025 28.000000000000004 26.125 20.849999999999998 65-69 25.924999999999997 28.560000000000002 25.755 19.759999999999998 70-74 24.585 28.585 26.695 20.135 75-79 24.01 27.515 27.63 20.845 80-84 25.355 27.834999999999997 26.095000000000002 20.715 85-89 24.545 28.384999999999998 26.334999999999997 20.735 90-94 25.095 27.41 27.615000000000002 19.88 95-99 25.355 28.005000000000003 26.745 19.895 100-104 26.290000000000003 28.185 25.759999999999998 19.765 105-109 25.564999999999998 28.37 26.685 19.38 110-114 26.02 27.894999999999996 25.985000000000003 20.1 115-119 25.7 28.315 26.66 19.325 120-124 25.711285564278214 27.966398319915996 26.466323316165806 19.85599279963998 125-129 27.055 28.17 25.674999999999997 19.1 130-134 26.995 28.095 25.255 19.655 135-139 27.597759775977597 28.17781778177818 25.777577757775777 18.44684468446845 140-144 28.130626125225046 27.58551710342068 25.695139027805563 18.58871774354871 145-149 27.21588635454182 27.581032412965182 26.865746298519404 18.33733493397359 150-151 29.89497374343586 26.081520380095025 26.431607901975497 17.591897974493623 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.5 5 0.5 6 0.0 7 0.0 8 0.5 9 0.5 10 0.0 11 0.0 12 1.0 13 2.0 14 1.5 15 1.0 16 0.5 17 1.0 18 1.5 19 1.5 20 1.0 21 1.0 22 1.0 23 2.5 24 3.5 25 1.5 26 1.0 27 4.0 28 6.5 29 4.5 30 6.0 31 9.0 32 10.5 33 19.5 34 35.0 35 50.0 36 70.5 37 83.0 38 122.0 39 173.0 40 192.0 41 220.0 42 233.5 43 246.0 44 274.0 45 261.0 46 245.0 47 257.0 48 257.5 49 228.0 50 169.0 51 121.0 52 116.0 53 111.5 54 82.0 55 62.0 56 51.5 57 42.5 58 31.5 59 21.5 60 23.0 61 20.5 62 12.0 63 7.5 64 7.5 65 5.0 66 2.0 67 6.0 68 6.5 69 3.5 70 2.5 71 0.5 72 0.0 73 1.0 74 1.5 75 1.0 76 0.5 77 0.5 78 1.0 79 2.0 80 1.5 81 1.5 82 3.0 83 2.5 84 4.0 85 4.5 86 4.0 87 4.5 88 3.5 89 3.0 90 3.0 91 3.0 92 1.5 93 1.5 94 1.5 95 0.5 96 1.0 97 0.5 98 0.5 99 1.0 100 5.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.025 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.005 125-129 0.0 130-134 0.0 135-139 0.01 140-144 0.02 145-149 0.04 150-151 0.025 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 60.650000000000006 #Duplication Level Percentage of deduplicated Percentage of total 1 64.13849958779885 38.9 2 20.774938169826875 25.2 3 8.32646331409728 15.15 4 3.5861500412201153 8.7 5 1.5251442704039573 4.625 6 0.8656224237427864 3.15 7 0.3297609233305853 1.4000000000000001 8 0.2061005770816158 1.0 9 0.12366034624896949 0.675 >10 0.12366034624896949 1.2 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGT 19 0.475 No Hit GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG 19 0.475 No Hit GCATATTTTGATCATGGAGGCATTCTACAATATGCCATCAGAAACCTGAT 10 0.25 No Hit ATTCCGCCCCCAGCAGCACCACCACCACCACCATGGCAATCAGTATTGAA 9 0.22499999999999998 No Hit TTCAGCTTTTATATCCCCTATTCTCTTCGTGAAGATGTCTTGCTGTGGAG 9 0.22499999999999998 No Hit GCTTATTAACCGTGGAGGGGAGAAGATATCACCAATTGAAGTGGATGCAG 9 0.22499999999999998 No Hit GGAGAAGCCTTTGAAAGAAAAGAAGCCAAGGACTCCTAGAGAAAAGAAGC 8 0.2 No Hit TGAAGCAGAATTCACCAAGTGTTGGATTGTTCACCCACCAATAGGGAACG 8 0.2 No Hit AAGCATTTCACCAGGCTGATCTGGCTTTGGAAAATGACTGCAGTGTGAGC 8 0.2 No Hit GTAGGAATGGGAGTATTTGCACTTGTGGTAACGGTATTTGCATTATTGAT 8 0.2 No Hit CAGGAGGGAGTGTGGCAGGGACAGCGTGCTGCAGTCCTTGACTGCTGTAT 8 0.2 No Hit GCTTGAGCAAATTCAGTTTCTAAGCAAAAGCTTTCCAGGCCCCTTTATCC 7 0.17500000000000002 No Hit CAGGTTAAATCTCCCATCTCTCTGGAGCAACTCACCTGGTAGTAAGGTTT 7 0.17500000000000002 No Hit GATGGGTTGCACTAATTGGCCTGACACTCTACATCATATTCTTCTCTCCA 7 0.17500000000000002 No Hit GCGTAGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGGCTAGACTA 7 0.17500000000000002 No Hit GCTAGTTACCGGACGGCAAAGCGAAGAAAGGGAATCCCCCACCGAGCCCC 7 0.17500000000000002 No Hit AGCAGCCCTTACTAATGAAAGTGCTACCAGCAAGTCAGTCTACTTTGCAC 7 0.17500000000000002 No Hit GAGAGACCGTGCACTGTGCTTGAAATTTGCTGAGATGCATCACTTCACAA 7 0.17500000000000002 No Hit GATAAATGTTCTTTTGTTCATGGTGATTTATGCCCTACCTGTGGAAAACA 7 0.17500000000000002 No Hit GTCCGATAAGCTCAGGCCACACAGAGAAAAGGCTAACTGGATAGAAACCA 6 0.15 No Hit CAGGATTTGGCAGTACACAATCGGAGGGAAAGCCGGGGTCTTTAAAGAGG 6 0.15 No Hit TGCAAATTTCAACACTCTGTGGAATGGATCTGTGGTGGCTCCACCTGATG 6 0.15 No Hit GGAAGCCTTGATGAGAAGATGTTGGTGCCAGGTGATGTTGCTGTGCCGAA 6 0.15 No Hit AATCGCTCTTGCCGTGCGAGATGCTAGCAAAAAGGTGTACTTCTCAGCGG 6 0.15 No Hit AACATAAGTGTCATTCAGCTTTTATATCCCCTATTCTCTTCGTGAAGATG 6 0.15 No Hit CATCTTGCAGCAACAAGAGAGCACGGCCTTGATGATTACCGGGTAGCTTG 6 0.15 No Hit CTGGAAAGAAACCGCTCCTAGAAGCAGAAGAACTGGAAGAAGGGAAAGAA 6 0.15 No Hit GCAAAGAAATCGAGGGTCAAGTGTTTTATCAAGCTTGTTAACTACCAACA 6 0.15 No Hit CACAAGACGCTGGGTTAGAAACCTGGATAGGGCATATTTCAAAATGTGGA 6 0.15 No Hit AATCTCTCCACTACTTCCTGCTTTTACTCCTACTGTTTATCTCTGTGAAT 6 0.15 No Hit AAAGAATAGGAAGAGAGGAAAGAACGAAGCCGATGACGAGAAGCGTGAGC 6 0.15 No Hit GAAGAATGGAAAATCCCTGTCTGAAGAGTTGCTGGCGTTTGACAGTCAGT 6 0.15 No Hit AAAGTAGCAGGATGCGGACACAGTAGGAGGTTGCTATGGTATTCTCAGGG 6 0.15 No Hit GAGAACTGGGTCCGCACCTATGTCGAGCATTACTACCCAGATTCAAGCAT 6 0.15 No Hit GCATTGGTATCTTTTCCCCACTTCCAAGCATTTTTTCAACTAATCTTATG 6 0.15 No Hit AATTGGAATATTTCAACCACGGAGGCATTCTTCCATATGCGATTAGGAGT 6 0.15 No Hit GTTGTGACAAGAAGACGAGCTTGAAGGTTAAAGTTCTGAAACGAACACGG 6 0.15 No Hit GAGATCCAACGGTCACTAGATAGCAACCGAAGAGAGAGTTCCTTAATTGT 6 0.15 No Hit TCATGTCGCCAGCTTCACGAGGAATGCTGCTGACAGGGATGATTCTTCTG 6 0.15 No Hit CTCCAATTGTAACCCCTGAGAAACTTGAGCTGCCTCCACTGCGTGAACTT 6 0.15 No Hit CCAGGCTGGTCAGACCAAGGAGGATATTGAGAAGACTCTGATGGTTCTTT 5 0.125 No Hit AAGCAATTTCTGCAATGTCACATATGGACTTTGACATGGCTTTGTATTAC 5 0.125 No Hit GTTACAGCTGAGGTTCAGAAAGTGCTTTCCTCATGAAATTGGGGCCCTTT 5 0.125 No Hit CTTGTCCACAAGGAGGCAAATGAAGCTTGATCACAATGACTTACTTGGAT 5 0.125 No Hit GTACAAGGTATCGAGCAGGAAAGAAGGGTACGAGATTAGAGAACTTGAGG 5 0.125 No Hit GTTTGGGGGCTTGATGATTATCATTTCTTGCCGTTTATATTTGGATCATC 5 0.125 No Hit AGCAAAGGTCTTAAATTAGGAATCTACTCAGATGCAGGGTATTTTACTTG 5 0.125 No Hit ATTTGAATGAATTTCTGCTTACTGTTTAATGCAGGCTGAAATCATGCTAA 5 0.125 No Hit GTGATGATGATTTAAGGTGTATGTTAAGAGAAGGTGATTTTGATGGTGAT 5 0.125 No Hit TGACCTCGATCCATCGCGTATAGGGACGCCCCCTGCTCGCGTTACTGCCA 5 0.125 No Hit CCTCCTCTGGATTATACTCAACAAAGGCCTTCGCAAGAGCTTGTAGCAAA 5 0.125 No Hit GAAAGTTTTCTGGCTTCCCATCACCACATCCTGGTATAATGCTGCCACTA 5 0.125 No Hit GGAACATTAAAGAAATCAGGGTTCAAGTCTTTTGAGGAGGGAAATGTCTG 5 0.125 No Hit TTGAGTTTCATGCGGTGGCCTCCAAGACTTCAGTTGTCAGTCCATCCATG 5 0.125 No Hit AATGGACGGGAGGTATCCCAATAGGAGGTTTCCTCCTATGGTTTTCAAAA 5 0.125 No Hit AAGGGTAAAAATGCTTATGCTTTGTCAGTGCTAAGGAGGATTGAGATGAA 5 0.125 No Hit TGACAAGCGTGCACATAGAGGGTCGAACCCGGGAGGAGGGGCGGAACAAG 5 0.125 No Hit CGAGGAAGAAGCCGAGATGGTTTCCCTCAAACTCCAAAAGCGGCTTGCAG 5 0.125 No Hit TTCTGAAAGACAAATGGAGTCCTGCTCTTCAGATACGAACCGTACTTTTG 5 0.125 No Hit GGCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATAGGAGGTTTC 5 0.125 No Hit TTTTGGCCATATACACCTTCCATCCAATTGTTGTATGGACTAAGAGAAGC 5 0.125 No Hit GGGAAGAGTTCTTTCGGAAAAGTAACAATCCAGATCGATCGGGTTGTAAT 5 0.125 No Hit TCAGTTACAAGCGCAAATCACTCGAAGCAGAAGCTTACTCATTTTAATTA 5 0.125 No Hit ATGGTTTCCCTCAAACTCCAAAAGCGGCTTGCAGCTAGTGTCCTAAAGTG 5 0.125 No Hit ACTCCGCCAATTCATCCTCCTCCTCCACTTCCTGGCTCTCCTCTCCTCCC 5 0.125 No Hit GCCTTGGCTTCCTTCCGCAGTCAAAACCGCGCAATTATCCCCGTCCTGAT 5 0.125 No Hit GGGAAATACGTACAATCTCACCTGCTTTCACGCTTAAGAGTTCTCCAAGA 5 0.125 No Hit GAAGGCATCCACCAAGAAGATAACTACGTTGGATGGTGGTGTGAAAATCT 5 0.125 No Hit GTCGATCTCTTTCTTCGCTTTTCTGTTTTTCTTTTATTTGAGATCATTTG 5 0.125 No Hit GATTTGTGTTGATATAGAAAACAATGGCACTGCATGGAAAGATTGAGACA 5 0.125 No Hit ACTCCCTCTGTAACTCTCTCTCTCTCCCTCTCTAGAACGTTTTTCGAACT 5 0.125 No Hit GAAACAACAAGATGCAATATCAAATGTCAAGACTCTTTCTGATGTCGAGG 5 0.125 No Hit CGTGCACAAGGGGGAGACACAAGGTGGGTACAACCACCAAGAGCAAGGTG 5 0.125 No Hit GTGAAACGGATGGGATTATGGAAATCCATCCGTTCAATTGCCCGCCTATA 5 0.125 No Hit GAAAAAATGGGTTACGTATTGAGAGTGAGATTGGCTTCATTCTTTGCAGG 5 0.125 No Hit GGATATCCGAACTTCATAGAAACAATTGCTGGTTCAGATCCTCCCAAAAA 5 0.125 No Hit CTTAACGATAATTTTAATGCAACAACTGCCGATACAGCAAGTGCTTCTGT 5 0.125 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.025 0.0 0.0 0.0 0.0 64-65 0.05 0.0 0.0 0.0 0.0 66-67 0.05 0.0 0.0 0.0 0.0 68-69 0.05 0.0 0.0 0.0 0.0 70-71 0.1125 0.0 0.0 0.0 0.0 72-73 0.15 0.0 0.0 0.0 0.0 74-75 0.16249999999999998 0.0 0.0 0.0 0.0 76-77 0.175 0.0 0.0 0.0 0.0 78-79 0.1875 0.0 0.0 0.0 0.0 80-81 0.325 0.0 0.0 0.0 0.0 82-83 0.6375 0.0 0.0 0.0 0.0 84-85 0.8374999999999999 0.0 0.0 0.0 0.0 86-87 1.05 0.0 0.0 0.0 0.0 88-89 1.1625 0.0 0.0 0.0 0.0 90-91 1.375 0.0 0.0 0.0 0.0 92-93 1.65 0.0 0.0 0.0 0.0 94-95 1.85 0.0 0.0 0.0 0.0 96-97 2.4625 0.0 0.0 0.0 0.0 98-99 2.9125 0.0 0.0 0.0 0.0 100-101 3.3 0.0 0.0 0.0 0.0 102-103 3.5375 0.0 0.0 0.0 0.0 104-105 3.8499999999999996 0.0 0.0 0.0 0.0 106-107 4.275 0.0 0.0 0.0 0.0 108-109 4.8125 0.0 0.0 0.0 0.0 110-111 5.25 0.0 0.0 0.0 0.0 112-113 5.762499999999999 0.0 0.0 0.0 0.0 114-115 6.5 0.0 0.0 0.0 0.0 116-117 7.125 0.0 0.0 0.0 0.0 118-119 7.925000000000001 0.0 0.0 0.0 0.0 120-121 9.037500000000001 0.0 0.0 0.0 0.0 122-123 10.0125 0.0 0.0 0.0 0.0 124-125 11.075 0.0 0.0 0.0 0.0 126-127 11.6125 0.0 0.0 0.0 0.0 128-129 12.825 0.0 0.0 0.0 0.0 130-131 13.725000000000001 0.0 0.0 0.0 0.0 132-133 14.2875 0.0 0.0 0.0 0.0 134-135 15.225 0.0 0.0 0.0 0.0 136-137 16.1125 0.0 0.0 0.0 0.0 138-139 16.725 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AAGAGTG 60 0.004491891 24.166666 145 AAAGAGT 55 4.8029233E-6 21.09091 140-144 GTAGGGA 45 6.5511256E-4 19.333332 135-139 TAGGGAA 45 6.5511256E-4 19.333332 135-139 GTCGTGT 50 0.0013298223 17.4 130-134 GAGCGTC 55 0.0025160722 15.818182 125-129 TCGTGTA 55 0.0025160722 15.818182 130-134 GAAAGAG 65 4.1823133E-4 15.615384 140-144 AGAGCGT 60 0.004491891 14.500001 125-129 GGAAAGA 60 0.004491891 14.500001 140-144 TCGGAAG 65 0.0076375785 13.384615 120-124 CGGAAGA 65 0.0076375785 13.384615 120-124 >>END_MODULE Read 1882892 spots for SRR26075363.sra Written 1882892 spots for SRR26075363.sra Read 1882892 spots for SRR26075363.sra Written 1882892 spots for SRR26075363.sra Read 1882892 spots for SRR26075363.sra Written 1882892 spots for SRR26075363.sra Read 1882892 spots for SRR26075363.sra Written 1882892 spots for SRR26075363.sra Read 1882892 spots for SRR26075363.sra Written 1882892 spots for SRR26075363.sra Read 1882892 spots for SRR26075363.sra Written 1882892 spots for SRR26075363.sra Read 1882892 spots for SRR26075363.sra Written 1882892 spots for SRR26075363.sra Read 1882892 spots for SRR26075363.sra Written 1882892 spots for SRR26075363.sra Read 1882892 spots for SRR26075363.sra Written 1882892 spots for SRR26075363.sra Read 1882892 spots for SRR26075363.sra Written 1882892 spots for SRR26075363.sra Read 1882892 spots for SRR26075363.sra Written 1882892 spots for SRR26075363.sra Read 1882892 spots for SRR26075363.sra Written 1882892 spots for SRR26075363.sra Read 1882892 spots for SRR26075363.sra Written 1882892 spots for SRR26075363.sra Read 1882892 spots for SRR26075363.sra Written 1882892 spots for SRR26075363.sra Read 1882901 spots for SRR26075363.sra Written 1882901 spots for SRR26075363.sra Read 1882892 spots for SRR26075363.sra Written 1882892 spots for SRR26075363.sra Read 1882892 spots for SRR26075363.sra Written 1882892 spots for SRR26075363.sra Read 1882892 spots for SRR26075363.sra Written 1882892 spots for SRR26075363.sra Read 1882892 spots for SRR26075363.sra Written 1882892 spots for SRR26075363.sra Read 1882892 spots for SRR26075363.sra Written 1882892 spots for SRR26075363.sra SRR ids: ['SRR26075363.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_ro1_iax5 SRR26075363.sra spots: 37657849 blocks: [[1, 1882892], [1882893, 3765784], [3765785, 5648676], [5648677, 7531568], [7531569, 9414460], [9414461, 11297352], [11297353, 13180244], [13180245, 15063136], [15063137, 16946028], [16946029, 18828920], [18828921, 20711812], [20711813, 22594704], [22594705, 24477596], [24477597, 26360488], [26360489, 28243380], [28243381, 30126272], [30126273, 32009164], [32009165, 33892056], [33892057, 35774948], [35774949, 37657849]] SRR26075363 file size 13907296 SRR26075363 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o SRR26075363 SRR26075363_1.fastq SRR26075363_2.fastq Input file: SRR26075363_1.fastq Paired file: SRR26075363_2.fastq trimmed: SRR26075363-trimmed-pair1.fastq, SRR26075363-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Tue Feb 11 22:39:54 2025 >> started Tue Feb 11 22:40:55 2025 >> done (61.090s) 37657849 read pairs processed; of these: 192 ( 0.00%) short read pairs filtered out after trimming by size control 333379 ( 0.89%) empty read pairs filtered out after trimming by size control 37324278 (99.11%) read pairs available; of these: 7582152 (20.31%) trimmed read pairs available after processing 29742126 (79.69%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 18 17 0.00% 19 7 0.00% 20 22 0.00% 21 26 0.00% 22 23 0.00% 23 29 0.00% 24 30 0.00% 25 40 0.00% 26 18 0.00% 27 37 0.00% 28 48 0.00% 29 50 0.00% 30 60 0.00% 31 47 0.00% 32 76 0.00% 33 62 0.00% 34 53 0.00% 35 61 0.00% 36 76 0.00% 37 64 0.00% 38 108 0.00% 39 95 0.00% 40 115 0.00% 41 128 0.00% 42 136 0.00% 43 118 0.00% 44 142 0.00% 45 151 0.00% 46 176 0.00% 47 238 0.00% 48 224 0.00% 49 271 0.00% 50 306 0.00% 51 320 0.00% 52 456 0.00% 53 424 0.00% 54 481 0.00% 55 589 0.00% 56 597 0.00% 57 734 0.00% 58 994 0.00% 59 1061 0.00% 60 1162 0.00% 61 1317 0.00% 62 1489 0.00% 63 1876 0.01% 64 1867 0.01% 65 2323 0.01% 66 2579 0.01% 67 2832 0.01% 68 3222 0.01% 69 3711 0.01% 70 4214 0.01% 71 4888 0.01% 72 5699 0.02% 73 6644 0.02% 74 7644 0.02% 75 8613 0.02% 76 9379 0.03% 77 10643 0.03% 78 11763 0.03% 79 13133 0.04% 80 14480 0.04% 81 15790 0.04% 82 18054 0.05% 83 20452 0.05% 84 23002 0.06% 85 25585 0.07% 86 27270 0.07% 87 29162 0.08% 88 30833 0.08% 89 32978 0.09% 90 35069 0.09% 91 38006 0.10% 92 40511 0.11% 93 44132 0.12% 94 47115 0.13% 95 51080 0.14% 96 54421 0.15% 97 57529 0.15% 98 58548 0.16% 99 61627 0.17% 100 63873 0.17% 101 66245 0.18% 102 69457 0.19% 103 72248 0.19% 104 76862 0.21% 105 80551 0.22% 106 84687 0.23% 107 87145 0.23% 108 89547 0.24% 109 93807 0.25% 110 92995 0.25% 111 95650 0.26% 112 98738 0.26% 113 102453 0.27% 114 105341 0.28% 115 110234 0.30% 116 112531 0.30% 117 115466 0.31% 118 119561 0.32% 119 120732 0.32% 120 122658 0.33% 121 125087 0.34% 122 126254 0.34% 123 130082 0.35% 124 132608 0.36% 125 134509 0.36% 126 139847 0.37% 127 143683 0.38% 128 144864 0.39% 129 148304 0.40% 130 148986 0.40% 131 150146 0.40% 132 151858 0.41% 133 154692 0.41% 134 154868 0.41% 135 157211 0.42% 136 159478 0.43% 137 161476 0.43% 138 166295 0.45% 139 169831 0.46% 140 171065 0.46% 141 171992 0.46% 142 173975 0.47% 143 174947 0.47% 144 176262 0.47% 145 179406 0.48% 146 179276 0.48% 147 179641 0.48% 148 183937 0.49% 149 184134 0.49% 150 187305 0.50% 151 29742126 79.69% 37324278 reads passed initial QC criterion=sequence-density sequence-density=0.43 sequence-density-rank=1 fanout-score=2.25 fanout-score-rank=33 prefix-density=0.44 prefix-fanout=2.2 sequence=AGGAAACCTCCT criterion=fanout-score sequence-density=0.04 sequence-density-rank=33 fanout-score=89.39 fanout-score-rank=1 prefix-density=0.33 prefix-fanout=10.2 sequence=CATCACCATCCCTGATTGATCTTTGATTCACAACAAGACCAACCAACCGTACAATGTTTACATAACTTGGGATAATCTCACAAGAAAGAAAGATCAATACAAAACTAGTCAGCTCAGGATGTTCCAGGCACGGTAGAACCGTAGAACCATAACAACAAGAGACATATTGCAGATGAGTACTGAAAAACAAAACACAGTACGTATTTACATGGGCAACCTTGGTTGAAGGCAACCTCATCAACGATGCTCGCTCTTCGTCTCTCCACTGTACATCCAGTCATAGACAGTGGGTGTGTTAGGCTGTGGCTTGTCAAAAACATGAGCACCAATATTCTTAGTAGCAAGGTTGCTACCAGGGTGGAACACGCTCCTCCAAACATTGCTACGCGCCGACACTGGGGTTGTAGGGGTCACTGGTGTCGTCGGTGTCCCTGGAGTTCCTGGCATAGTCATGGACCTCTGAAACTTATTAACAGGACTGCTCCCCTCTCCGACGTCAATATCTTTGATG criterion=sequence-density sequence-density=0.46 sequence-density-rank=1 fanout-score=2.01 fanout-score-rank=28 prefix-density=0.47 prefix-fanout=2.0 sequence=ACGCCACGACTTGCTTGTGAGTGATGAGATGATATGAATGTCCTCGATCGTGAGTCTTGTGAGTCTTTTGAGTTGTATGATAAAAAAGGATGCCCTACTGTACTAGAATAAATTATATGTCATAAATGGCCTCCTAATTTAAATAAACTGGG criterion=fanout-score sequence-density=0.01 sequence-density-rank=32 fanout-score=104.51 fanout-score-rank=1 prefix-density=0.14 prefix-fanout=5.3 sequence=ACAAAGCAGTTGCATTTATCTAAAGTATT SRR26075363 testing PE reads STAR mapping to Ensembl genome Started job on | Feb 11 22:41:37 Started mapping on | Feb 11 22:41:38 Finished on | Feb 11 22:48:56 Mapping speed, Million of reads per hour | 306.77 Number of input reads | 37324278 Average input read length | 291 UNIQUE READS: Uniquely mapped reads number | 32986676 Uniquely mapped reads % | 88.38% Average mapped length | 289.76 Number of splices: Total | 28785130 Number of splices: Annotated (sjdb) | 28038764 Number of splices: GT/AG | 28265953 Number of splices: GC/AG | 394517 Number of splices: AT/AC | 29581 Number of splices: Non-canonical | 95079 Mismatch rate per base, % | 0.40% Deletion rate per base | 0.03% Deletion average length | 3.07 Insertion rate per base | 0.02% Insertion average length | 2.50 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 986225 % of reads mapped to multiple loci | 2.64% Number of reads mapped to too many loci | 117126 % of reads mapped to too many loci | 0.31% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 8.26% % of reads unmapped: other | 0.41% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 3351377 3351377 3351377 N_multimapping 986225 986225 986225 N_noFeature 850485 32573484 1082683 N_ambiguous 403002 2798 220188 UnstrandedReadsAssigned:31733189 PositiveStrandReadsAssigned:410394 NegativeStrandReadsAssigned:31683805 Dataset is classified negative stranded MeadianReadLen=151 20thPercentileLength=148 echo kmer=143 SRR26075363 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,400 [index] number of k-mers: 62,057,036 [index] number of equivalence classes: 130,681 [quant] running in paired-end mode [quant] will process pair 1: SRR26075363-trimmed-pair1.fastq SRR26075363-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 37,324,278 reads, 32,175,975 reads pseudoaligned [quant] estimated average fragment length: 203.745 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,163 rounds 52401 SRR26075363.ke.tsv 34699 SRR26075363.se.tsv 87100 total ==> SRR26075363.ke.tsv <== target_id length eff_length est_counts tpm Potri.005G200100.1.v4.1 2018 1815.25 3559 50.3988 Potri.005G024800.1.v4.1 1035 832.255 1981 61.1868 Potri.004G059700.1.v4.1 961 758.255 19 0.644122 Potri.007G009000.2.v4.1 1416 1213.25 0 0 Potri.003G141000.2.v4.1 2943 2740.25 1207 11.3226 Potri.016G087400.1.v4.1 270 96.1223 3845 1028.26 Potri.015G069301.1.v4.1 564 362.735 0 0 Potri.010G195200.1.v4.1 1773 1570.25 404 6.61364 Potri.012G127500.1.v4.1 977 774.255 6936 230.279 ==> SRR26075363.se.tsv <== Potri.001G166300.v4.1 0 Potri.001G448400.v4.1 1026 Potri.001G233950.v4.1 0 Potri.001G122700.v4.1 557 Potri.001G212900.v4.1 0 Potri.001G182400.v4.1 0 Potri.001G256600.v4.1 0 Potri.001G040500.v4.1 37 Potri.001G416900.v4.1 0 Potri.001G452600.v4.1 1793 SRR26075363 completed mapping pipeline successfully