Starting /dee2/code/volunteer_pipeline.sh SRR26075364 current disk space = 3052322291712 free memory = 1579182356 SRR26075364 SRAfilesize bdefb1770b2982e5d17795754dc3d3c4 SRR26075364.sra SRR26075364.sra file validated SRR26075364 is paired end SRR26075364 is conventional basespace SRR26075364 read1 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR26075364_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.63175 37.0 37.0 37.0 37.0 37.0 2 36.496 37.0 37.0 37.0 37.0 37.0 3 36.5815 37.0 37.0 37.0 37.0 37.0 4 36.659 37.0 37.0 37.0 37.0 37.0 5 36.659 37.0 37.0 37.0 37.0 37.0 6 36.5705 37.0 37.0 37.0 37.0 37.0 7 36.626 37.0 37.0 37.0 37.0 37.0 8 36.654 37.0 37.0 37.0 37.0 37.0 9 36.571 37.0 37.0 37.0 37.0 37.0 10-14 36.625150000000005 37.0 37.0 37.0 37.0 37.0 15-19 36.5887 37.0 37.0 37.0 37.0 37.0 20-24 36.5138 37.0 37.0 37.0 37.0 37.0 25-29 36.432599999999994 37.0 37.0 37.0 37.0 37.0 30-34 36.398399999999995 37.0 37.0 37.0 37.0 37.0 35-39 36.3112 37.0 37.0 37.0 37.0 37.0 40-44 36.2762 37.0 37.0 37.0 37.0 37.0 45-49 36.2292 37.0 37.0 37.0 37.0 37.0 50-54 36.1023 37.0 37.0 37.0 37.0 37.0 55-59 36.0253 37.0 37.0 37.0 37.0 37.0 60-64 35.982600000000005 37.0 37.0 37.0 37.0 37.0 65-69 35.900999999999996 37.0 37.0 37.0 37.0 37.0 70-74 35.9859 37.0 37.0 37.0 37.0 37.0 75-79 35.9573 37.0 37.0 37.0 37.0 37.0 80-84 35.983999999999995 37.0 37.0 37.0 37.0 37.0 85-89 35.8893 37.0 37.0 37.0 37.0 37.0 90-94 35.743900000000004 37.0 37.0 37.0 37.0 37.0 95-99 35.789 37.0 37.0 37.0 37.0 37.0 100-104 35.770799999999994 37.0 37.0 37.0 37.0 37.0 105-109 35.6595 37.0 37.0 37.0 37.0 37.0 110-114 35.61560000000001 37.0 37.0 37.0 37.0 37.0 115-119 35.509699999999995 37.0 37.0 37.0 37.0 37.0 120-124 35.4516 37.0 37.0 37.0 37.0 37.0 125-129 35.3936 37.0 37.0 37.0 37.0 37.0 130-134 35.2832 37.0 37.0 37.0 32.2 37.0 135-139 35.1866 37.0 37.0 37.0 34.6 37.0 140-144 35.056999999999995 37.0 37.0 37.0 25.0 37.0 145-149 35.065799999999996 37.0 37.0 37.0 25.0 37.0 150-151 34.99575 37.0 37.0 37.0 31.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.0 1101 2 0.0 1101 3 0.0 1101 4 0.0 1101 5 0.0 1101 6 0.0 1101 7 0.0 1101 8 0.0 1101 9 0.0 1101 10-14 0.0 1101 15-19 0.0 1101 20-24 0.0 1101 25-29 0.0 1101 30-34 0.0 1101 35-39 0.0 1101 40-44 0.0 1101 45-49 0.0 1101 50-54 0.0 1101 55-59 0.0 1101 60-64 0.0 1101 65-69 0.0 1101 70-74 0.0 1101 75-79 0.0 1101 80-84 0.0 1101 85-89 0.0 1101 90-94 0.0 1101 95-99 0.0 1101 100-104 0.0 1101 105-109 0.0 1101 110-114 0.0 1101 115-119 0.0 1101 120-124 0.0 1101 125-129 0.0 1101 130-134 0.0 1101 135-139 0.0 1101 140-144 0.0 1101 145-149 0.0 1101 150-151 0.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 17 1.0 18 0.0 19 1.0 20 1.0 21 6.0 22 6.0 23 6.0 24 9.0 25 11.0 26 9.0 27 13.0 28 25.0 29 24.0 30 36.0 31 43.0 32 60.0 33 118.0 34 149.0 35 409.0 36 2868.0 37 205.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 44.875970934602854 14.006514657980457 8.243547982961664 32.87396642445503 2 19.950000000000003 14.025000000000002 32.35 33.675 3 17.125 18.099999999999998 29.825000000000003 34.949999999999996 4 19.075 25.575 27.05 28.299999999999997 5 21.45 31.35 26.150000000000002 21.05 6 21.775 33.825 24.375 20.025000000000002 7 14.575 29.075 38.800000000000004 17.549999999999997 8 17.65 28.125 30.425 23.799999999999997 9 18.625 23.724999999999998 35.625 22.025 10-14 19.36596829841492 30.346517325866294 27.40137006850343 22.88614430721536 15-19 19.634999999999998 29.13 27.525 23.71 20-24 19.825 28.335 28.705000000000002 23.135 25-29 19.325 28.655 28.24 23.78 30-34 20.055 28.88 27.62 23.445 35-39 20.835 27.6 28.73 22.835 40-44 19.46 28.465 27.655 24.42 45-49 20.125 27.944999999999997 28.71 23.22 50-54 20.22 27.3 29.28 23.200000000000003 55-59 19.895 28.375 27.650000000000002 24.08 60-64 21.235 28.845 27.0 22.919999999999998 65-69 19.625 29.315 27.46 23.599999999999998 70-74 21.415 27.77 28.175 22.64 75-79 20.955 28.24 27.625 23.18 80-84 20.630000000000003 28.62 27.384999999999998 23.365 85-89 20.565 27.455000000000002 28.43 23.549999999999997 90-94 20.549999999999997 26.369999999999997 28.265 24.815 95-99 20.835 27.994999999999997 27.095000000000002 24.075 100-104 21.135 27.805000000000003 28.050000000000004 23.01 105-109 20.735 27.505000000000003 27.644999999999996 24.115000000000002 110-114 20.955 28.360000000000003 26.93 23.755000000000003 115-119 21.27 27.37 27.200000000000003 24.16 120-124 21.465 27.955000000000002 26.27 24.310000000000002 125-129 21.145 27.794999999999998 27.125 23.935000000000002 130-134 20.865000000000002 28.02 27.150000000000002 23.965 135-139 21.255 27.355 26.86 24.529999999999998 140-144 21.43 28.92 25.990000000000002 23.66 145-149 21.515 28.175 26.700000000000003 23.61 150-151 23.05 26.674999999999997 25.75 24.525 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 1.0 2 1.5 3 1.0 4 1.0 5 1.0 6 1.5 7 1.5 8 2.0 9 2.0 10 0.5 11 1.5 12 2.5 13 1.5 14 0.5 15 0.0 16 0.0 17 0.5 18 0.5 19 0.0 20 1.0 21 2.5 22 2.0 23 1.0 24 3.0 25 3.5 26 4.0 27 8.5 28 10.5 29 14.5 30 18.0 31 23.5 32 37.0 33 47.0 34 59.5 35 74.5 36 99.0 37 132.0 38 137.0 39 139.5 40 177.5 41 209.5 42 211.5 43 229.5 44 252.0 45 263.5 46 262.5 47 228.5 48 211.5 49 223.0 50 181.0 51 136.5 52 129.5 53 108.5 54 85.5 55 62.0 56 51.5 57 34.0 58 19.0 59 16.5 60 12.5 61 10.5 62 6.5 63 6.5 64 5.5 65 2.5 66 3.0 67 4.5 68 4.5 69 4.0 70 3.0 71 2.0 72 1.5 73 1.5 74 0.5 75 0.0 76 0.0 77 0.0 78 0.5 79 0.5 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.22499999999999998 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.005 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150-151 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 61.35 #Duplication Level Percentage of deduplicated Percentage of total 1 63.77343113284434 39.125 2 20.986145069274652 25.75 3 8.842705786471068 16.275000000000002 4 3.3007334963325183 8.1 5 1.7114914425427872 5.25 6 0.8964955175224123 3.3000000000000003 7 0.40749796251018744 1.7500000000000002 8 0.040749796251018745 0.2 9 0.0 0.0 >10 0.040749796251018745 0.25 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTTCTATTTAAACTACGAGAGCTTTCCCACCAAAAATTAGCTAAGGTATG 10 0.25 No Hit CTCCTGGCACTCTGCTAGCTTTTCTGCGGCAGCTGTTATTTCTTTCTCCT 8 0.2 No Hit GCTCGTGTATACTGAATTGACAGATATTTCAACTTCCAGGAGAGTTCATA 7 0.17500000000000002 No Hit GTGGTCTGCAGTATGGACCTCCACTCATGCTCCATACCTTCGCCCTAAAG 7 0.17500000000000002 No Hit CCGTAGGAAAGCCGGCCATCATGGTCTGAATCAAGGTGGGCGAAGCGGTC 7 0.17500000000000002 No Hit ATCAAAATACGAATCCTTTTTGCTGCCGGTAGAGGGCTCATAACTTCATC 7 0.17500000000000002 No Hit TCAAAGATGGCCATATGGTACAACCAACTCATAGCTCAAGCCCCTCCTAT 7 0.17500000000000002 No Hit GCCATGAACCCAAGGTTTCACTAACTCGTACAAACTAGAGTTTCTTTTTT 7 0.17500000000000002 No Hit TTCTACTAACCGATGAACCAAATCCTACAGACTTGTCCCACTGAGCATTC 7 0.17500000000000002 No Hit GCCTAACAAAAGTCAAAAAAAATTAAACACGTAGCTTAGTACTTAATTCT 7 0.17500000000000002 No Hit TTTTTTTTTTAGTTAAAAAAATTGCTGTATCGATGATCCAATGTCTAATC 7 0.17500000000000002 No Hit AGGCTAGTTGCAAAGAGTAGGTAATTACTAGCAAAGAAGAGGCAGCTTCA 7 0.17500000000000002 No Hit GCTTCGATCTCTTCCTTGATTCGTTCAAACACACTTGGTCCTTTAACTTC 6 0.15 No Hit GCACCAGATCATACAACCACCCCCTCTGAAAACTCAAGAATAAAAATAAA 6 0.15 No Hit AAGGATTCCCCAACTCACTGCGTTTAACACTCCATGAACATTTCTCTTGC 6 0.15 No Hit AGAGCCCCGCCGGTGTTGGCAATGATCATGGTTCTGCAGACCCCAGCAAT 6 0.15 No Hit GCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGA 6 0.15 No Hit GCGTAACCTCAAGTCCCCAGGTGGCATGGGTGGAGCTGTGCACTCTTTAG 6 0.15 No Hit ACGGTCCATGGCTAAAGCCCAAGCAGCATGTTCAGAGAGTGTCTTTGCCA 6 0.15 No Hit CTGCATGCGCTTGTGGGCACGGCCTCTAGGCTTCTTCTTCTTATCTTGCT 6 0.15 No Hit CGTGTTCAGTGCGACCGTACAGCTCTGGAACCCAAAGGTTCGTTTTTTTC 6 0.15 No Hit GGCTTTCAGCTGATGCTCCAGCTTCTCCTTCTCTGCCTTCAGCCTCTGCT 6 0.15 No Hit GTCTGAGAGCTTCTCGAGCTGTTAACCTGTCCATGGGATCGTACCTGAGC 6 0.15 No Hit AGGGAAACCAACAGCTAGCATGATCAGCTGAATCTTCAATAAAAGGAAGG 6 0.15 No Hit CGTAAGGATCATAGAGGGTGTTGAAGAAAAATGGAGTTGATGGCGAGAGA 6 0.15 No Hit CTCCCATTTCTTGTATTTTACCTCTGCTGCCTCTTGTGTGTCTAGCATGT 6 0.15 No Hit GTCGACATATCGGACCAGTCACCCGATATTTGTGGCAAAACACTAGTAGG 6 0.15 No Hit ATGTCATGAATGAGCCAAAGACGGCTTTGTTCCACCCAAAACGCCAATGG 6 0.15 No Hit CCGAGGAATTATAAACCCTGAGAATAGGTTCCATATTCCATAAAATGCAG 6 0.15 No Hit AGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGGGATGAGC 6 0.15 No Hit TTCTCATTTCTTCTTGTGCTCTCTTCATTTCTTCAGGATCCATGTTTTCC 6 0.15 No Hit GTACAAGAAGTTTCAAAATGAAAAGGGCAAATCTACTTGTTTGTATCTTA 6 0.15 No Hit GGCAACTGAAACTTCAAAATATTCAAACTAGGAATCATGGTACTCAATGA 6 0.15 No Hit GACTGGCCACTGTTTCCCATCAGGTAACTGAAGTTTCATAAACCCCGAAA 6 0.15 No Hit CTTTTCTTCTTCTTCGTCTTCTTCTTCTTGTGCCTTTCCTCGTTTTGGTT 5 0.125 No Hit CAATGACCATAAACAACTTTTACTCTGAAAGCTGAGCTCCATCTCATGAA 5 0.125 No Hit TGTCAAGATCCGACGATGGAGGCCATATTGAGATGCAAAAACCATTCCCG 5 0.125 No Hit TTGACAAACTAAGAGATGACACAAGCATCTGGGTTTTCCTCTTCTGATCA 5 0.125 No Hit CTCCACAATCTCTTCACTGCTCCCAGGCTCCTGAGATCCAAATTGATTGC 5 0.125 No Hit AGGCAAAACATCCATGCATCAGAAACTGGTATAGAGACCTTAATCAAGCT 5 0.125 No Hit ATCTGTCCTGACCTTGCCATCAACAAGGACATGTCTCTGCATCAAAATAG 5 0.125 No Hit GGACTAGCAGCATTGATTGAAACATAACATGACAAAAAAAAAATTGATCT 5 0.125 No Hit TTCTCTTGCCAATATTACAAATAGGAATCCTCGAACACCAAACAACCAAA 5 0.125 No Hit GTCTCCTGGCTTCTTCGGATCTTCTTTCTTCTTTTCAGGCTCTTTTGCTG 5 0.125 No Hit GGCCTTCGTCCAACCGTAGCAGGCATCGAGACGGTTGAATCCACACTCAA 5 0.125 No Hit GCCACCTTCCTTTCCATCGAGTCCCACTTCCTCATCTTTTCTATCCTGGC 5 0.125 No Hit TACAGGACTAAAGGTAACACAACTGGAGGAGGTTATGCATACAAGAAAGC 5 0.125 No Hit CTAGAAAAATATATAATCTTCAACCACAGTTTCTGTAGATGGGTTGAATT 5 0.125 No Hit AAATCAGCTAAATGTTTTTGCAAAGCCATATAGTTATCGCATACAACACC 5 0.125 No Hit GAGGAGAAGAGGATGAGCAGAAGCAGCCGATTTGCAACGAAGCAGATCCA 5 0.125 No Hit CTGTGTTAAAACTTATTCTGTATATTCCTGGGTTCACAACATCAACAATG 5 0.125 No Hit GCCCCCACCCATATCAGCTCCAGCACCTTGATACATCTTAGCAATGATAG 5 0.125 No Hit GTAAATTCTGTCACCGTCAAAATTATAATCAAGGCCTGTTCGAATACCAC 5 0.125 No Hit TGGGACACAAAAAGGCACAAAATCAGTGCTACAATCTCTGAGAAGTTATC 5 0.125 No Hit GTCGAGATCGATGACCCCTTCTATTACTCCGTGCTAAGGGCTTCGTCGAT 5 0.125 No Hit GAGAATGATGCGTCGATGTTGAGAGTAATTAAGACAAGCTCTATGTTTTG 5 0.125 No Hit GACCGTTTGGTAGGAGGTCTCCCAATAGCTCTCATCAAATTGGCTACCTC 5 0.125 No Hit CTCATAAACATCTTCATGATTTCAAATCCCCACCAATTCCCCCAACAACA 5 0.125 No Hit CTTTGTATAAATAAGTGGGAGAAGAAATCTAAAATTAATTCAACTTATGC 5 0.125 No Hit GCTCGGTTCACCGTAAGAGATGACCCTCGCCACAGGCTAGCAACACCTTC 5 0.125 No Hit GAAATTTGGGAAGCAGTTAAAGTTTTAGAAAATAATGGATGCAGGGATAT 5 0.125 No Hit GTACAGTCTCTGTAGAAGAGGAGGCTGTGAAGCAGTGTTGAATGAAGAGA 5 0.125 No Hit GCGATTCGCACGGTATCGAGTTCCAGTTTCAAAACTGTCTCTGTGTCCCC 5 0.125 No Hit CCACCTCGTACAAATTCAAACATAAGATTGCTCAAACTGGACATCCTTGG 5 0.125 No Hit ACCGAGTGGAGTGTGGATTCCTTCTGGATGTTGTAGTCAGCAAGGGTGCG 5 0.125 No Hit GCCAACGGTCGGGGGACAAAGTAGGTCATGAATCTGACAGATCCTTCTGT 5 0.125 No Hit ACTAGTTAACATCGATAGAACATCTGACATAGTAGGCCTATCGTTCACAT 5 0.125 No Hit GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTGAGCCATCTCGTAT 5 0.125 TruSeq Adapter, Index 25 (97% over 38bp) CCACTGGCAACAATACTCTTGGCGGCTTGCCTGACAATGTAAGCTCCACT 5 0.125 No Hit GGCATGAGCATGAGGTACGTGGCATTGGAAACCAAGTAGTGGTAACCAAG 5 0.125 No Hit GCACTAAATTTCTACTTGCAAAAAGCCCCATCTTTGCCCTAAACTGTGCC 5 0.125 No Hit AACGTTACTAGCTTCTCCCTCAGGTGCAGGAAAATCCTCGGCAAGTTTGC 5 0.125 No Hit CATGGGCAAGACTTCACATCCTTGTACAGAATGTATATCCCTGTACGCTT 5 0.125 No Hit GGGATAATTGCGCGGTTTTGACTGCGGAAGGAAGCCAAGGCCCACCTAAA 5 0.125 No Hit GCCTCACTTAGTTACAGTTTTATGGATAATTGGGATATTCTTTGGTATAG 5 0.125 No Hit CCCACACTGTGGACACTCGTAGAAATTATCAATTGCAAAACTGGCAGAAG 5 0.125 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0 0.0 0.0 0.0 0.0 64-65 0.025 0.0 0.0 0.0 0.0 66-67 0.037500000000000006 0.0 0.0 0.0 0.0 68-69 0.05 0.0 0.0 0.0 0.0 70-71 0.05 0.0 0.0 0.0 0.0 72-73 0.05 0.0 0.0 0.0 0.0 74-75 0.075 0.0 0.0 0.0 0.0 76-77 0.1 0.0 0.0 0.0 0.0 78-79 0.125 0.0 0.0 0.0 0.0 80-81 0.16249999999999998 0.0 0.0 0.0 0.0 82-83 0.1875 0.0 0.0 0.0 0.0 84-85 0.2 0.0 0.0 0.0 0.0 86-87 0.2 0.0 0.0 0.0 0.0 88-89 0.30000000000000004 0.0 0.0 0.0 0.0 90-91 0.425 0.0 0.0 0.0 0.0 92-93 0.5125 0.0 0.0 0.0 0.0 94-95 0.675 0.0 0.0 0.0 0.0 96-97 0.825 0.0 0.0 0.0 0.0 98-99 1.075 0.0 0.0 0.0 0.0 100-101 1.2 0.0 0.0 0.0 0.0 102-103 1.325 0.0 0.0 0.0 0.0 104-105 1.425 0.0 0.0 0.0 0.0 106-107 1.6625 0.0 0.0 0.0 0.0 108-109 2.025 0.0 0.0 0.0 0.0 110-111 2.425 0.0 0.0 0.0 0.0 112-113 2.8 0.0 0.0 0.0 0.0 114-115 3.3875 0.0 0.0 0.0 0.0 116-117 3.875 0.0 0.0 0.0 0.0 118-119 4.1375 0.0 0.0 0.0 0.0 120-121 4.675000000000001 0.0 0.0 0.0 0.0 122-123 5.175 0.0 0.0 0.0 0.0 124-125 5.5625 0.0 0.0 0.0 0.0 126-127 5.8375 0.0 0.0 0.0 0.0 128-129 6.2625 0.0 0.0 0.0 0.0 130-131 6.949999999999999 0.0 0.0 0.0 0.0 132-133 7.387499999999999 0.0 0.0 0.0 0.0 134-135 8.1 0.0 0.0 0.0 0.0 136-137 8.675 0.0 0.0 0.0 0.0 138-139 9.399999999999999 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTTGATG 10 0.006830828 145.0 2 GATGTAG 10 0.006830828 145.0 5 TAGTCCT 10 0.006830828 145.0 9 TGTAGTC 10 0.006830828 145.0 7 GCCATGA 10 0.006830828 145.0 1 GTAGTCC 10 0.006830828 145.0 8 TGATGTA 10 0.006830828 145.0 4 >>END_MODULE SRR26075364 read2 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR26075364_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.0835 37.0 37.0 37.0 37.0 37.0 2 36.154 37.0 37.0 37.0 37.0 37.0 3 36.107 37.0 37.0 37.0 37.0 37.0 4 36.0485 37.0 37.0 37.0 37.0 37.0 5 36.03 37.0 37.0 37.0 37.0 37.0 6 35.869 37.0 37.0 37.0 37.0 37.0 7 36.0225 37.0 37.0 37.0 37.0 37.0 8 36.0275 37.0 37.0 37.0 37.0 37.0 9 36.017 37.0 37.0 37.0 37.0 37.0 10-14 35.954 37.0 37.0 37.0 37.0 37.0 15-19 35.8322 37.0 37.0 37.0 37.0 37.0 20-24 35.825599999999994 37.0 37.0 37.0 37.0 37.0 25-29 35.7094 37.0 37.0 37.0 37.0 37.0 30-34 35.53689999999999 37.0 37.0 37.0 37.0 37.0 35-39 35.4702 37.0 37.0 37.0 37.0 37.0 40-44 35.3721 37.0 37.0 37.0 37.0 37.0 45-49 35.3438 37.0 37.0 37.0 37.0 37.0 50-54 35.115899999999996 37.0 37.0 37.0 37.0 37.0 55-59 35.1336 37.0 37.0 37.0 37.0 37.0 60-64 35.1964 37.0 37.0 37.0 37.0 37.0 65-69 35.133500000000005 37.0 37.0 37.0 34.6 37.0 70-74 35.0434 37.0 37.0 37.0 29.8 37.0 75-79 34.9414 37.0 37.0 37.0 25.0 37.0 80-84 35.0087 37.0 37.0 37.0 32.2 37.0 85-89 34.99569999999999 37.0 37.0 37.0 25.0 37.0 90-94 34.872499999999995 37.0 37.0 37.0 25.0 37.0 95-99 34.894400000000005 37.0 37.0 37.0 25.0 37.0 100-104 34.8079 37.0 37.0 37.0 25.0 37.0 105-109 34.8401 37.0 37.0 37.0 25.0 37.0 110-114 34.7664 37.0 37.0 37.0 25.0 37.0 115-119 34.7906 37.0 37.0 37.0 25.0 37.0 120-124 34.580600000000004 37.0 37.0 37.0 25.0 37.0 125-129 34.568599999999996 37.0 37.0 37.0 25.0 37.0 130-134 34.5634 37.0 37.0 37.0 25.0 37.0 135-139 34.475199999999994 37.0 37.0 37.0 25.0 37.0 140-144 34.3803 37.0 37.0 37.0 25.0 37.0 145-149 34.3842 37.0 37.0 37.0 25.0 37.0 150-151 34.12825 37.0 37.0 37.0 25.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.0 1101 2 0.0 1101 3 0.0 1101 4 0.0 1101 5 0.0 1101 6 0.0 1101 7 0.0 1101 8 0.0 1101 9 0.0 1101 10-14 0.0 1101 15-19 0.0 1101 20-24 0.0 1101 25-29 0.0 1101 30-34 0.0 1101 35-39 0.0 1101 40-44 0.0 1101 45-49 0.0 1101 50-54 0.0 1101 55-59 0.0 1101 60-64 0.0 1101 65-69 0.0 1101 70-74 0.0 1101 75-79 0.0 1101 80-84 0.0 1101 85-89 0.0 1101 90-94 0.0 1101 95-99 0.0 1101 100-104 0.0 1101 105-109 0.0 1101 110-114 0.0 1101 115-119 0.0 1101 120-124 0.0 1101 125-129 0.0 1101 130-134 0.0 1101 135-139 0.0 1101 140-144 0.0 1101 145-149 0.0 1101 150-151 0.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 12 1.0 13 5.0 14 15.0 15 17.0 16 18.0 17 8.0 18 17.0 19 20.0 20 23.0 21 11.0 22 25.0 23 15.0 24 18.0 25 20.0 26 19.0 27 14.0 28 20.0 29 16.0 30 30.0 31 37.0 32 45.0 33 107.0 34 169.0 35 613.0 36 2524.0 37 193.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 48.525 22.85 9.525 19.1 2 31.424999999999997 23.525 25.624999999999996 19.425 3 25.7 27.750000000000004 29.049999999999997 17.5 4 27.375 32.1 24.099999999999998 16.425 5 29.175 33.900000000000006 19.425 17.5 6 27.900000000000002 36.15 20.05 15.9 7 24.6 23.549999999999997 33.15 18.7 8 25.2 26.075 26.325 22.400000000000002 9 26.174999999999997 25.424999999999997 27.375 21.025 10-14 26.72 28.749999999999996 23.599999999999998 20.93 15-19 26.615 28.645 25.635 19.105 20-24 25.86 29.43 25.180000000000003 19.53 25-29 26.009999999999998 28.68 25.83 19.48 30-34 25.855 28.365000000000002 25.61 20.169999999999998 35-39 25.369999999999997 28.26 25.89 20.48 40-44 26.26 28.685 25.929999999999996 19.125 45-49 25.645 28.9 25.395 20.06 50-54 23.794999999999998 28.525 28.1 19.580000000000002 55-59 25.205 28.465 26.405 19.925 60-64 25.590000000000003 27.58 27.384999999999998 19.445 65-69 25.585 28.16 26.625 19.63 70-74 25.005 29.345 26.515 19.134999999999998 75-79 23.815 29.470000000000002 26.55 20.165 80-84 25.22 28.410000000000004 26.39 19.98 85-89 24.945 28.43 27.165 19.46 90-94 24.8 28.360000000000003 26.900000000000002 19.939999999999998 95-99 25.205 28.625 26.939999999999998 19.23 100-104 24.91 28.605000000000004 27.065 19.42 105-109 24.884999999999998 29.59 26.215 19.31 110-114 24.81 30.0 25.900000000000002 19.29 115-119 24.445 28.96 26.645000000000003 19.950000000000003 120-124 25.679999999999996 29.9 25.83 18.59 125-129 26.040000000000003 29.365000000000002 25.805 18.790000000000003 130-134 26.805 29.865000000000002 25.0 18.33 135-139 26.150000000000002 29.755 25.705 18.39 140-144 25.874999999999996 30.03 26.21 17.885 145-149 26.155 29.085 26.424999999999997 18.335 150-151 26.7125 29.4875 25.575 18.224999999999998 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.5 2 0.5 3 0.5 4 0.5 5 0.5 6 1.5 7 2.0 8 2.0 9 3.5 10 3.5 11 1.5 12 1.5 13 2.0 14 3.5 15 3.5 16 1.5 17 1.5 18 2.0 19 4.0 20 5.0 21 3.5 22 2.5 23 1.5 24 4.0 25 5.0 26 4.5 27 7.5 28 8.0 29 8.0 30 11.0 31 15.0 32 25.5 33 35.0 34 43.5 35 58.0 36 67.0 37 97.5 38 138.0 39 158.5 40 180.0 41 205.5 42 206.0 43 243.0 44 271.0 45 251.0 46 266.5 47 254.5 48 220.0 49 189.0 50 162.5 51 142.0 52 117.5 53 110.0 54 96.0 55 62.0 56 46.5 57 36.5 58 23.5 59 16.5 60 11.5 61 11.0 62 7.5 63 9.0 64 5.5 65 1.5 66 4.0 67 4.5 68 1.5 69 0.5 70 1.5 71 2.5 72 3.0 73 2.0 74 2.0 75 3.0 76 2.0 77 1.5 78 1.5 79 2.0 80 3.5 81 3.5 82 4.5 83 4.0 84 3.5 85 3.0 86 1.5 87 1.5 88 2.0 89 2.0 90 0.5 91 2.5 92 3.5 93 3.0 94 3.0 95 2.5 96 2.0 97 1.5 98 5.5 99 6.5 100 15.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150-151 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 62.150000000000006 #Duplication Level Percentage of deduplicated Percentage of total 1 66.13032984714401 41.099999999999994 2 19.750603378921962 24.55 3 8.246178600160901 15.375 4 3.1375703942075623 7.8 5 1.6090104585679808 5.0 6 0.5631536604987932 2.1 7 0.36202735317779566 1.575 8 0.04022526146419952 0.2 9 0.08045052292839903 0.44999999999999996 >10 0.04022526146419952 0.27499999999999997 >50 0.04022526146419952 1.575 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG 63 1.575 No Hit GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGT 11 0.27499999999999997 No Hit AGTTGCATGAGGACAGTTCTAATGTGCTTCTCAATCTTGCAAGTTCCCAG 9 0.22499999999999998 No Hit CCCAAGCTGCTCACAGTATACGGGCGTCGGCATCCAGACCGTCGGCTGAT 9 0.22499999999999998 No Hit TCTGCGGGTGAAAACAGAAACTTTGGAAAGTGAGCTTCAAGAAGAAAAGA 8 0.2 No Hit AGCACGTTGCAATCTGAAAGACATTATCATGATGTTTACTCTTCTGGATC 7 0.17500000000000002 No Hit AGAGATCTTAAACCAAAGAATATTTTGGCAAATGCAAATTGCAAACTTAA 7 0.17500000000000002 No Hit TCTCTCTAAAGCTTCCCTTTAATTTACGGTTTTAAACCTTCTCTAGTTCT 7 0.17500000000000002 No Hit GAAAAGATTATATTCAGCTCTTCACTAACTGCGGCAATATGGAGAGAGAA 7 0.17500000000000002 No Hit GACAGCTCTCAACCCCTACAATGCTCATAAAAAAACATATATAAAAAAAC 7 0.17500000000000002 No Hit TTTACAAAAGCGGAGACCCCGCCAAACGCGCCCGAGCTATTGTTATGGCG 7 0.17500000000000002 No Hit GCGGTTTCAAAACATGGTAAACAACATTTTGTCAGTGTTGGCATATGGCG 7 0.17500000000000002 No Hit GTTTGCTCAAATCATAAGAGGCTTGAACAATTTGTCTTGTCCGAAATCCC 7 0.17500000000000002 No Hit CTCCACCGTCCACAGTACTCCACGCTTGGAAGGAATCTGGAAAGGAAAGA 7 0.17500000000000002 No Hit TGAGTGGAGGTAGTCCTAGTCCACATTCTACCACTGGACCAAATATGCAA 6 0.15 No Hit CGTAAGGATCCATCTGGTAAAGCTATCAATGCACTTGAGCAGCACATCAA 6 0.15 No Hit ATCATGTAGTGCATCTAGCTCCAAAGCATGTAGGGAAAAGCTGCGAAGGG 6 0.15 No Hit CCTGGGCACATGGCCAGCAATTGTCCAAATGAGGGAATCTGCCACACCTG 6 0.15 No Hit ACTTGTAATCAAGAGGCTTCCAGTATTTTACAAGCAAAGAGATCTTCAAT 6 0.15 No Hit GACTGGTTGGTGTCAACCGTGGCACAAGACTAGCCGAAGTTGCTATGACG 6 0.15 No Hit CGATCAAGAACTGCTTGGAATGCATAGATGTCTTAATGAAGGCATGCATT 6 0.15 No Hit GCGCCAAGCCTTGTTAGTGAAATAATGCAACTCGCGCAAAAGAAAAATGC 6 0.15 No Hit GGTATTAGAGCCATTAAAGGAAGAGCAGTATTATGAGATTAGGGAGCCCT 6 0.15 No Hit CAAAAATATCCTTCACCATCAGAAGAAAAGGATGTAAAAGCGCCAAATGT 6 0.15 No Hit GTAGTACTTGGAGGGCATTAGTCCTGCTGTTTTGTGACTTAGGAGTAGCT 6 0.15 No Hit AGCTACCTCGACTCCAGAATCTAGTAATGCAACATGTGGACCATTCAGCT 6 0.15 No Hit ATGGACGGGAGGTATCCCAATAGGAGGTTTCCTCCTATGGTTTTCAAAAC 6 0.15 No Hit TAAGATGAATGAGGCAAGAAAAGCCAATCAAACTGCTATGGTATCTGAAA 6 0.15 No Hit CTTGCTTCAACAATAACGTCTCTTTCAGAAGGCATTGGTATCTTTTCCCC 5 0.125 No Hit AGTAAAACCTATATCTTGTGTCCTCTTGTTACACCACCGTCAATTATTCG 5 0.125 No Hit AAGAGAGAATAATAGTAGTAGTAATGGGGTTGGCCAAGGATAGAGATGAT 5 0.125 No Hit AAAACAAGAGCTTACAGTAATCCGAAAGAGAGGTTTCCAGGGACCCCAAA 5 0.125 No Hit GAAATGAGCTTTAGATTTTATGAAAGTGCTTCTGCAAGAAAGAGAACTGT 5 0.125 No Hit CATAAAAAAACTCAGCCCGAAACGATGGTTCAGCTCCAAAAAAGACCGAT 5 0.125 No Hit AGCTGATGTGGCAATGGTTAGAATGCAAGCAGACGGACGTCTACCTTCGT 5 0.125 No Hit CTCTCTCTAAGAATAGGGATCGAGTGCGAAAGTCTCCGTTTCGGGTGGAT 5 0.125 No Hit CAAAACCCAACTTCTTCTTCCAAATGGGGTGGCTTCAGTCCTTCGTCTCC 5 0.125 No Hit CCGAGAAGTACAAGTCAGAAGATGAGGAGCACAAGAAGAAGGTCGAGTCC 5 0.125 No Hit CGACCGTTCAGCGTGGAACATGAAGTACGGAGACGATAATTATGCCAATT 5 0.125 No Hit GTAATGGTTGCTGCTCTCAGGAACGTAGTTTCTGGCACCAGCTCAACTGA 5 0.125 No Hit GACAACACCCGTGTCTAACGGAAGTGGCAGGGTTCATGAGTGCTCAATCT 5 0.125 No Hit GGTTAAAGATGGCTTTAGGGTCAGTTTTATTGGAGATTCTGCATAGACCT 5 0.125 No Hit CTGGAAATTACATGAAAACCAAAGAAATCCTCGGGAACAGTGCTGCACAG 5 0.125 No Hit CAGTATTTTAGTGATAACACATTAAACAGTTCAAAAGAGTTAAGGGCAAA 5 0.125 No Hit AGCTCGTATTCTTGATGAACTCGAAACACATGGATCTGATGATAAAAGTA 5 0.125 No Hit AAATGATGAAAGCTGTGTTGAAATTGGATTTACACGATGACAAAGACAAG 5 0.125 No Hit AGGAGTCTACCCTTCATTTGGTGCTCCGATTGAGAGGTGGCATGCAGATC 5 0.125 No Hit CCAGCTGCCGGTGTTGAGGTATGCCTAGAAATATGGAAGGGAACTCAACC 5 0.125 No Hit AATTAGGGCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATAGGA 5 0.125 No Hit GGGCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATAGGAGGTTT 5 0.125 No Hit ACAAAGCCAAGAAAAAAGCCACGAAGACAGCCTCTAGTCTTTCAGGGGAC 5 0.125 No Hit TGGCGGATGCTCTTGGTCCTAACACAAAGATACTGTTCAAAGACAACGCA 5 0.125 No Hit GTTGGACGCCAGTAGTAGTGATGTTGGAGGTCAAGAAAGTGGTGTGGAAT 5 0.125 No Hit GTTTATTTTTAGAAATGATGAGAGCTGTTTGGTTGGGTGGTTCTAGTCAA 5 0.125 No Hit ATCCCTTCAAGCATGTCAATGAAAGCACTCAAGTTCAGAAAATCAAAAAA 5 0.125 No Hit GAGCTATGGAAAGAAGGTAACCAATTTGAGCTAGTGGATTCAACATTGAG 5 0.125 No Hit AATGAGCGATCTAGTGAACCATATTGGACATTGCATTTCAGAACAGATGA 5 0.125 No Hit GGGAGGTATCCCAATAGGAGGTTTCCTCCTATGGTTTTCAAAACAATCAC 5 0.125 No Hit GAAAAAGAAAAAACCCTCTGAAGGGTCAACACTGACGTCTTGATTTCTTC 5 0.125 No Hit GCGAGAACAAGCTGACGGATTTGAGCGGAGTAAGTGTGAAGATTTTGTTT 5 0.125 No Hit GCAAAAAGAAGAATGTATTATTTCAGGCCAGTTTGAGAAAGATTGCAAGC 5 0.125 No Hit CCAACATCAATCATCATGCCCTCCTCCTCCTCCTCGCCCCTCTCACTCTC 5 0.125 No Hit GAGAGATTCATATTCATTTGTATCCAAGGTGCTGAATCTTGGAGGAGGTT 5 0.125 No Hit AGAGGAAATTAGAGGAGAAAAGAGCAAGAGCCTCAGAGAAGATGCAGAAT 5 0.125 No Hit GCTTGACAAGCTTGGTGGTGCATTTGCACCCAAGCCATCATCTGGACCCC 5 0.125 No Hit GGAGCTGACCCAGCTATATGCAAAATACAAGGATCAAGGTTTGGAGATTC 5 0.125 No Hit GGAGGTCTTATCAAAACCGATTGGAGCCAAGCACCGTTTACAGCTTCTTA 5 0.125 No Hit CCTCTATGAGGGACTTGACTGGCTATCTAACAACATTGCTAACAAGGCTT 5 0.125 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0 0.0 0.0 0.0 0.0 64-65 0.025 0.0 0.0 0.0 0.0 66-67 0.037500000000000006 0.0 0.0 0.0 0.0 68-69 0.05 0.0 0.0 0.0 0.0 70-71 0.05 0.0 0.0 0.0 0.0 72-73 0.05 0.0 0.0 0.0 0.0 74-75 0.075 0.0 0.0 0.0 0.0 76-77 0.1 0.0 0.0 0.0 0.0 78-79 0.125 0.0 0.0 0.0 0.0 80-81 0.16249999999999998 0.0 0.0 0.0 0.0 82-83 0.1875 0.0 0.0 0.0 0.0 84-85 0.2 0.0 0.0 0.0 0.0 86-87 0.2 0.0 0.0 0.0 0.0 88-89 0.30000000000000004 0.0 0.0 0.0 0.0 90-91 0.425 0.0 0.0 0.0 0.0 92-93 0.5125 0.0 0.0 0.0 0.0 94-95 0.675 0.0 0.0 0.0 0.0 96-97 0.825 0.0 0.0 0.0 0.0 98-99 1.075 0.0 0.0 0.0 0.0 100-101 1.2 0.0 0.0 0.0 0.0 102-103 1.35 0.0 0.0 0.0 0.0 104-105 1.45 0.0 0.0 0.0 0.0 106-107 1.7 0.0 0.0 0.0 0.0 108-109 2.1 0.0 0.0 0.0 0.0 110-111 2.5374999999999996 0.0 0.0 0.0 0.0 112-113 2.925 0.0 0.0 0.0 0.0 114-115 3.5125 0.0 0.0 0.0 0.0 116-117 4.0 0.0 0.0 0.0 0.0 118-119 4.300000000000001 0.0 0.0 0.0 0.0 120-121 4.85 0.0 0.0 0.0 0.0 122-123 5.3375 0.0 0.0 0.0 0.0 124-125 5.8 0.0 0.0 0.0 0.0 126-127 6.112500000000001 0.0 0.0 0.0 0.0 128-129 6.5125 0.0 0.0 0.0 0.0 130-131 7.199999999999999 0.0 0.0 0.0 0.0 132-133 7.637499999999999 0.0 0.0 0.0 0.0 134-135 8.35 0.0 0.0 0.0 0.0 136-137 8.9125 0.0 0.0 0.0 0.0 138-139 9.675 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GAAATAC 10 0.006830828 145.0 4 CCTAATG 10 0.006830828 145.0 2 ATGGACT 10 0.006830828 145.0 6 GCTGATG 10 0.006830828 145.0 145 ACCTAAT 10 0.006830828 145.0 1 GACTTGG 10 0.006830828 145.0 9 TGAAATA 10 0.006830828 145.0 3 >>END_MODULE Read 830104 spots for SRR26075364.sra Written 830104 spots for SRR26075364.sra Read 830104 spots for SRR26075364.sra Written 830104 spots for SRR26075364.sra Read 830104 spots for SRR26075364.sra Written 830104 spots for SRR26075364.sra Read 830104 spots for SRR26075364.sra Written 830104 spots for SRR26075364.sra Read 830104 spots for SRR26075364.sra Written 830104 spots for SRR26075364.sra Read 830104 spots for SRR26075364.sra Written 830104 spots for SRR26075364.sra Read 830104 spots for SRR26075364.sra Written 830104 spots for SRR26075364.sra Read 830104 spots for SRR26075364.sra Written 830104 spots for SRR26075364.sra Read 830104 spots for SRR26075364.sra Written 830104 spots for SRR26075364.sra Read 830104 spots for SRR26075364.sra Written 830104 spots for SRR26075364.sra Read 830104 spots for SRR26075364.sra Written 830104 spots for SRR26075364.sra Read 830104 spots for SRR26075364.sra Written 830104 spots for SRR26075364.sra Read 830104 spots for SRR26075364.sra Written 830104 spots for SRR26075364.sra Read 830104 spots for SRR26075364.sra Written 830104 spots for SRR26075364.sra Read 830122 spots for SRR26075364.sra Written 830122 spots for SRR26075364.sra Read 830104 spots for SRR26075364.sra Written 830104 spots for SRR26075364.sra Read 830104 spots for SRR26075364.sra Written 830104 spots for SRR26075364.sra Read 830104 spots for SRR26075364.sra Written 830104 spots for SRR26075364.sra Read 830104 spots for SRR26075364.sra Written 830104 spots for SRR26075364.sra Read 830104 spots for SRR26075364.sra Written 830104 spots for SRR26075364.sra SRR ids: ['SRR26075364.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_7x49zjl8 SRR26075364.sra spots: 16602098 blocks: [[1, 830104], [830105, 1660208], [1660209, 2490312], [2490313, 3320416], [3320417, 4150520], [4150521, 4980624], [4980625, 5810728], [5810729, 6640832], [6640833, 7470936], [7470937, 8301040], [8301041, 9131144], [9131145, 9961248], [9961249, 10791352], [10791353, 11621456], [11621457, 12451560], [12451561, 13281664], [13281665, 14111768], [14111769, 14941872], [14941873, 15771976], [15771977, 16602098]] SRR26075364 file size 6125202 SRR26075364 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o SRR26075364 SRR26075364_1.fastq SRR26075364_2.fastq Input file: SRR26075364_1.fastq Paired file: SRR26075364_2.fastq trimmed: SRR26075364-trimmed-pair1.fastq, SRR26075364-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Tue Feb 11 23:15:36 2025 >> started Tue Feb 11 23:15:56 2025 >> done (20.339s) 16602098 read pairs processed; of these: 75 ( 0.00%) short read pairs filtered out after trimming by size control 60396 ( 0.36%) empty read pairs filtered out after trimming by size control 16541627 (99.64%) read pairs available; of these: 2409841 (14.57%) trimmed read pairs available after processing 14131786 (85.43%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 18 7 0.00% 19 6 0.00% 20 13 0.00% 21 20 0.00% 22 15 0.00% 23 20 0.00% 24 15 0.00% 25 26 0.00% 26 28 0.00% 27 20 0.00% 28 30 0.00% 29 32 0.00% 30 21 0.00% 31 28 0.00% 32 24 0.00% 33 17 0.00% 34 28 0.00% 35 21 0.00% 36 35 0.00% 37 41 0.00% 38 32 0.00% 39 39 0.00% 40 39 0.00% 41 39 0.00% 42 46 0.00% 43 61 0.00% 44 38 0.00% 45 66 0.00% 46 48 0.00% 47 80 0.00% 48 55 0.00% 49 74 0.00% 50 91 0.00% 51 100 0.00% 52 108 0.00% 53 112 0.00% 54 139 0.00% 55 135 0.00% 56 145 0.00% 57 174 0.00% 58 219 0.00% 59 200 0.00% 60 281 0.00% 61 303 0.00% 62 361 0.00% 63 370 0.00% 64 363 0.00% 65 457 0.00% 66 449 0.00% 67 569 0.00% 68 630 0.00% 69 760 0.00% 70 791 0.00% 71 964 0.01% 72 1147 0.01% 73 1192 0.01% 74 1466 0.01% 75 1699 0.01% 76 1802 0.01% 77 1921 0.01% 78 2138 0.01% 79 2517 0.02% 80 2767 0.02% 81 3265 0.02% 82 3683 0.02% 83 4257 0.03% 84 4732 0.03% 85 5079 0.03% 86 5547 0.03% 87 6067 0.04% 88 6429 0.04% 89 7269 0.04% 90 7901 0.05% 91 8684 0.05% 92 9346 0.06% 93 10490 0.06% 94 11221 0.07% 95 12220 0.07% 96 12841 0.08% 97 13677 0.08% 98 14057 0.08% 99 14971 0.09% 100 15898 0.10% 101 16983 0.10% 102 18300 0.11% 103 19532 0.12% 104 20823 0.13% 105 22145 0.13% 106 22927 0.14% 107 23477 0.14% 108 24757 0.15% 109 25149 0.15% 110 25900 0.16% 111 27203 0.16% 112 28602 0.17% 113 30567 0.18% 114 31891 0.19% 115 32920 0.20% 116 33928 0.21% 117 35365 0.21% 118 36133 0.22% 119 36388 0.22% 120 37760 0.23% 121 38798 0.23% 122 39268 0.24% 123 41465 0.25% 124 43174 0.26% 125 44413 0.27% 126 45906 0.28% 127 46339 0.28% 128 47976 0.29% 129 47866 0.29% 130 48581 0.29% 131 49451 0.30% 132 50275 0.30% 133 52988 0.32% 134 54549 0.33% 135 56532 0.34% 136 56892 0.34% 137 57933 0.35% 138 58224 0.35% 139 58465 0.35% 140 59094 0.36% 141 60217 0.36% 142 61799 0.37% 143 63283 0.38% 144 65351 0.40% 145 66125 0.40% 146 67389 0.41% 147 67717 0.41% 148 68489 0.41% 149 68279 0.41% 150 69185 0.42% 151 14131786 85.43% 16541627 reads passed initial QC criterion=sequence-density sequence-density=0.32 sequence-density-rank=1 fanout-score=24.82 fanout-score-rank=6 prefix-density=0.30 prefix-fanout=24.8 sequence=GGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTGAGCCATCTCGTATGCCGTCTTCTGCTTG criterion=fanout-score sequence-density=0.03 sequence-density-rank=39 fanout-score=150.89 fanout-score-rank=1 prefix-density=0.35 prefix-fanout=11.9 sequence=CATCACCATCCCTGATTGATCTTTGATTCACAACAAGACCAACCAACCGTACAATGTTTACATAACTTGGGATAATCTCACAAGAAAGAAAGATCAATACAAAACTAGTCAGCTCAGGATGTTCCAGGCACGGTAGAACCGTAGAACCATAACAACAAGAGACATATTGCAGATGAGTACTGAAAAACAAAACACAGTACGTATTTACATGGGCAACCTTGGTTGAAGGCAACCTCATCAACGATGCTCGCTCTTCGTCTCTCCACTGTACATCCAGTCATAGACAGTGGGTGTGTTAGGCTGTGGCTTGTCAAAAACATGAGCACCAATATTCTTAGTAGCAAGGTTGCTACCAGGGTGGAACACGCTCCTCCAAACATTGCTACGCGCCGACACTGGGGTTGTAGGGGTCACTGGTGTCGTCGGTGTCCCTGGAGTTCCTGGCATAGTCATGGACCTCTGAAACTTATTAACAGGACTGCTCCCCTCTCCGACGTCAATATCTTTGATG criterion=sequence-density sequence-density=0.21 sequence-density-rank=1 fanout-score=2.43 fanout-score-rank=33 prefix-density=0.21 prefix-fanout=2.4 sequence=ATGTACCCTGACTTAGGTTTCTCAGAGA criterion=fanout-score sequence-density=0.01 sequence-density-rank=37 fanout-score=43.17 fanout-score-rank=1 prefix-density=0.35 prefix-fanout=1.5 sequence=TCTTTGAGAGTGCATAGATTTGTGTTGATATAGAAAACAATGGCACTACATGGAAAGATTGAGACAACATTAGAACTCAAGTCCTCCGCAGAGAAGTTCTACAAAGTGTGGAGGAGCCAGTCCTTCCATGTTCCCAAACATGCTTCCAAGCATATCCAAGGAGTTGATATACATGCAGGTGACTGGGAGACTGCGGGCTCTATCAGGATTTGGCAGTACACAATCGGAGGGAAAGCCGGGGTCTTTAAAGAGGAGGTTTCC SRR26075364 testing PE reads STAR mapping to Ensembl genome Started job on | Feb 11 23:16:44 Started mapping on | Feb 11 23:16:44 Finished on | Feb 11 23:20:41 Mapping speed, Million of reads per hour | 251.27 Number of input reads | 16541627 Average input read length | 295 UNIQUE READS: Uniquely mapped reads number | 13671635 Uniquely mapped reads % | 82.65% Average mapped length | 293.09 Number of splices: Total | 11176066 Number of splices: Annotated (sjdb) | 10870409 Number of splices: GT/AG | 10976493 Number of splices: GC/AG | 143762 Number of splices: AT/AC | 13313 Number of splices: Non-canonical | 42498 Mismatch rate per base, % | 0.41% Deletion rate per base | 0.03% Deletion average length | 3.11 Insertion rate per base | 0.02% Insertion average length | 2.54 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 510702 % of reads mapped to multiple loci | 3.09% Number of reads mapped to too many loci | 34942 % of reads mapped to too many loci | 0.21% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 13.21% % of reads unmapped: other | 0.84% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 2359290 2359290 2359290 N_multimapping 510702 510702 510702 N_noFeature 402682 13516775 481782 N_ambiguous 165151 815 89187 UnstrandedReadsAssigned:13103802 PositiveStrandReadsAssigned:154045 NegativeStrandReadsAssigned:13100666 Dataset is classified negative stranded MeadianReadLen=151 20thPercentileLength=151 echo kmer=147 SRR26075364 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,400 [index] number of k-mers: 62,057,036 [index] number of equivalence classes: 130,681 [quant] running in paired-end mode [quant] will process pair 1: SRR26075364-trimmed-pair1.fastq SRR26075364-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 16,541,627 reads, 13,573,218 reads pseudoaligned [quant] estimated average fragment length: 213.334 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,180 rounds 52401 SRR26075364.ke.tsv 34699 SRR26075364.se.tsv 87100 total ==> SRR26075364.ke.tsv <== target_id length eff_length est_counts tpm Potri.005G200100.1.v4.1 2018 1805.67 2564 84.4791 Potri.005G024800.1.v4.1 1035 822.666 6872 496.968 Potri.004G059700.1.v4.1 961 748.666 1 0.0794659 Potri.007G009000.2.v4.1 1416 1203.67 0 0 Potri.003G141000.2.v4.1 2943 2730.67 618.423 13.4737 Potri.016G087400.1.v4.1 270 88.2538 1058 713.216 Potri.015G069301.1.v4.1 564 353.337 0 0 Potri.010G195200.1.v4.1 1773 1560.67 76 2.89716 Potri.012G127500.1.v4.1 977 764.666 2691 209.368 ==> SRR26075364.se.tsv <== Potri.001G166300.v4.1 0 Potri.001G448400.v4.1 102 Potri.001G233950.v4.1 0 Potri.001G122700.v4.1 188 Potri.001G212900.v4.1 0 Potri.001G182400.v4.1 0 Potri.001G256600.v4.1 0 Potri.001G040500.v4.1 57 Potri.001G416900.v4.1 0 Potri.001G452600.v4.1 329 SRR26075364 completed mapping pipeline successfully