Starting /dee2/code/volunteer_pipeline.sh SRR26075365 current disk space = 3052328026112 free memory = 1576806484 SRR26075365 SRAfilesize 030f19a9aebcd58316045b21dc6594e4 SRR26075365.sra SRR26075365.sra file validated SRR26075365 is paired end SRR26075365 is conventional basespace SRR26075365 read1 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR26075365_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.59 37.0 37.0 37.0 37.0 37.0 2 36.611 37.0 37.0 37.0 37.0 37.0 3 36.5815 37.0 37.0 37.0 37.0 37.0 4 36.607 37.0 37.0 37.0 37.0 37.0 5 36.6855 37.0 37.0 37.0 37.0 37.0 6 36.614 37.0 37.0 37.0 37.0 37.0 7 36.5555 37.0 37.0 37.0 37.0 37.0 8 36.6355 37.0 37.0 37.0 37.0 37.0 9 36.6465 37.0 37.0 37.0 37.0 37.0 10-14 36.6683 37.0 37.0 37.0 37.0 37.0 15-19 36.5496 37.0 37.0 37.0 37.0 37.0 20-24 36.533699999999996 37.0 37.0 37.0 37.0 37.0 25-29 36.4982 37.0 37.0 37.0 37.0 37.0 30-34 36.4342 37.0 37.0 37.0 37.0 37.0 35-39 36.4385 37.0 37.0 37.0 37.0 37.0 40-44 36.30460000000001 37.0 37.0 37.0 37.0 37.0 45-49 36.1789 37.0 37.0 37.0 37.0 37.0 50-54 36.2211 37.0 37.0 37.0 37.0 37.0 55-59 36.07019999999999 37.0 37.0 37.0 37.0 37.0 60-64 36.056 37.0 37.0 37.0 37.0 37.0 65-69 35.9259 37.0 37.0 37.0 37.0 37.0 70-74 36.02460000000001 37.0 37.0 37.0 37.0 37.0 75-79 36.0341 37.0 37.0 37.0 37.0 37.0 80-84 36.046299999999995 37.0 37.0 37.0 37.0 37.0 85-89 35.976600000000005 37.0 37.0 37.0 37.0 37.0 90-94 35.815099999999994 37.0 37.0 37.0 37.0 37.0 95-99 35.8866 37.0 37.0 37.0 37.0 37.0 100-104 35.8309 37.0 37.0 37.0 37.0 37.0 105-109 35.744499999999995 37.0 37.0 37.0 37.0 37.0 110-114 35.624 37.0 37.0 37.0 37.0 37.0 115-119 35.5466 37.0 37.0 37.0 37.0 37.0 120-124 35.534000000000006 37.0 37.0 37.0 37.0 37.0 125-129 35.4299 37.0 37.0 37.0 37.0 37.0 130-134 35.28529999999999 37.0 37.0 37.0 34.6 37.0 135-139 35.18140000000001 37.0 37.0 37.0 29.8 37.0 140-144 34.9714 37.0 37.0 37.0 25.0 37.0 145-149 35.0135 37.0 37.0 37.0 27.4 37.0 150-151 34.733000000000004 37.0 37.0 37.0 25.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.0 1101 2 0.0 1101 3 0.0 1101 4 0.0 1101 5 0.0 1101 6 0.0 1101 7 0.0 1101 8 0.0 1101 9 0.0 1101 10-14 0.0 1101 15-19 0.0 1101 20-24 0.0 1101 25-29 0.0 1101 30-34 0.0 1101 35-39 0.0 1101 40-44 0.0 1101 45-49 0.0 1101 50-54 0.0 1101 55-59 0.0 1101 60-64 0.0 1101 65-69 0.0 1101 70-74 0.0 1101 75-79 0.0 1101 80-84 0.0 1101 85-89 0.0 1101 90-94 0.0 1101 95-99 0.0 1101 100-104 0.0 1101 105-109 0.0 1101 110-114 0.0 1101 115-119 0.0 1101 120-124 0.0 1101 125-129 0.0 1101 130-134 0.0 1101 135-139 0.0 1101 140-144 0.0 1101 145-149 0.0 1101 150-151 0.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 22 4.0 23 5.0 24 9.0 25 8.0 26 5.0 27 17.0 28 19.0 29 24.0 30 28.0 31 36.0 32 73.0 33 131.0 34 196.0 35 437.0 36 2782.0 37 226.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 43.512024048096194 15.030060120240481 8.71743486973948 32.74048096192384 2 21.125 14.6 33.650000000000006 30.625000000000004 3 20.075000000000003 15.45 30.7 33.775 4 21.95 23.849999999999998 25.75 28.449999999999996 5 23.325000000000003 30.15 24.825 21.7 6 20.150000000000002 33.1 24.725 22.025 7 13.350000000000001 29.925 40.6 16.125 8 16.775000000000002 27.400000000000002 30.95 24.875 9 17.974999999999998 25.3 33.7 23.025000000000002 10-14 19.400000000000002 30.919999999999998 26.75 22.93 15-19 20.31 27.765 28.134999999999998 23.79 20-24 20.165 29.189999999999998 27.089999999999996 23.555 25-29 20.18 29.885 27.029999999999998 22.905 30-34 19.845 29.03 27.68 23.445 35-39 19.6 29.4 26.424999999999997 24.575 40-44 20.1 28.275 28.205000000000002 23.419999999999998 45-49 20.385 28.910000000000004 26.72 23.985 50-54 19.53 29.520000000000003 26.91 24.04 55-59 19.965 29.235 27.555000000000003 23.244999999999997 60-64 19.36 28.18 29.354999999999997 23.105 65-69 20.32 28.044999999999998 27.55 24.085 70-74 20.87 27.36 26.939999999999998 24.83 75-79 21.455 27.779999999999998 27.750000000000004 23.015 80-84 21.235 28.32 26.779999999999998 23.665 85-89 21.6 28.134999999999998 26.63 23.635 90-94 21.05 27.845 26.889999999999997 24.215 95-99 22.759999999999998 28.265 26.26 22.715 100-104 21.55 27.644999999999996 26.69 24.115000000000002 105-109 22.25 28.025 25.47 24.255 110-114 21.985 28.555000000000003 26.47 22.99 115-119 22.34 27.435 26.77 23.455000000000002 120-124 21.88 27.639999999999997 26.51 23.97 125-129 22.7 28.49 25.014999999999997 23.794999999999998 130-134 21.85 27.01 26.575 24.565 135-139 22.25 27.33 25.95 24.47 140-144 22.86 27.29 26.51 23.34 145-149 22.195 27.775 25.590000000000003 24.44 150-151 21.775 27.3 26.25 24.675 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.5 10 0.5 11 0.0 12 0.0 13 1.0 14 2.0 15 1.0 16 0.0 17 1.5 18 1.5 19 0.5 20 1.0 21 1.5 22 2.5 23 5.5 24 6.5 25 4.5 26 5.0 27 7.0 28 9.5 29 18.5 30 23.5 31 26.0 32 41.0 33 43.0 34 41.0 35 74.0 36 95.5 37 104.0 38 112.5 39 135.0 40 191.0 41 214.5 42 222.0 43 218.5 44 231.0 45 268.5 46 270.0 47 263.0 48 249.5 49 220.5 50 170.0 51 132.5 52 116.0 53 94.5 54 77.0 55 58.0 56 46.0 57 40.5 58 29.0 59 19.0 60 16.5 61 11.0 62 9.0 63 7.5 64 5.0 65 8.0 66 10.5 67 11.5 68 10.5 69 6.0 70 4.0 71 2.0 72 0.0 73 1.0 74 1.0 75 0.0 76 0.0 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150-151 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 57.475 #Duplication Level Percentage of deduplicated Percentage of total 1 60.28708133971292 34.65 2 21.748586341887776 25.0 3 9.438886472379297 16.275000000000002 4 4.784688995215311 11.0 5 1.9573727707699 5.625 6 0.9569377990430622 3.3000000000000003 7 0.5654632448890822 2.275 8 0.13049151805132667 0.6 9 0.04349717268377556 0.22499999999999998 >10 0.08699434536755112 1.05 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAACGCTTATCTCGTAT 26 0.65 TruSeq Adapter, Index 9 (97% over 37bp) GGATGATATAGGAAATGGAGTGGCCACTAGTGGGAATCCGGAATAGAGCT 16 0.4 No Hit TGCATACATGGAGTACTGACCACCTGGTTTACTATAGGCCACAATAACTA 9 0.22499999999999998 No Hit GGGTAGAATAAGATTGATGTTGAGAAGCTTAGCCTTGATAACGGTGCAAA 8 0.2 No Hit GTGTGTCTGTTGGTTAAGGTCAGGTGTTGCTTGTGAACCCTGTGGTGCAC 8 0.2 No Hit TCCTCAAAAACTTCAGGTTCTGCCTCATCACATCACGCAGATCATCATCA 8 0.2 No Hit GCCTTCCTTCTTGTACTGCTGGATAGCGGCGGAGATGAGTTCGCCGACGG 7 0.17500000000000002 No Hit TGGCCTAATTCAGGGTCCTGCACCACTGTACCATTACAGCAGAACTCTTT 7 0.17500000000000002 No Hit CCGCATTCTCATCCTCTGTGTCATCTTCATCATCATCGTCCATTCCAGGA 7 0.17500000000000002 No Hit CTCAATCTAACGTACAAGCTCAATATCAAAGAATGGTACAAGCAAAGGAA 7 0.17500000000000002 No Hit GCCACCACGAAGCCTCAGAACCAGATGCAGTGTTGACTCCTTCTGGATAT 7 0.17500000000000002 No Hit GGATACTCCCTAGGCCCAAATATGGTAGAACCAATTCTCACATTCGTACT 7 0.17500000000000002 No Hit GGGAAATATCTATATTGTTTAAAAACGCCATCACTCCTTGCAGGGAACTT 7 0.17500000000000002 No Hit CTTGCCACAAGCGCCCTTAAGCAAATCAATATTGTCTGCGAAAGCAACGT 7 0.17500000000000002 No Hit CCGGGAGCTTGGCCGGTGCTTGAACCGTCATAGTTCCACTTTGGAAGCTT 7 0.17500000000000002 No Hit TGGGGATTTGTAATAATAAGGTGGTGGAGGAGATGGCGATGGTGGAGGGG 7 0.17500000000000002 No Hit CTCATCATCCTCCTCGCGTTTGGATTTCATGCCCACTTGACGATCAGAAG 7 0.17500000000000002 No Hit GGATGTTGCAGAGATATCATGAAAGACCAGATCAGTCAAATGGATTCCAT 7 0.17500000000000002 No Hit CACAACTCCAGCAACGAATACCGTTACTAACCCAACGGCGAAGATCGTAC 7 0.17500000000000002 No Hit GCTCGGAGTAAGGCTCGATCCCAAGCTTGCTTCCATCAAACACAGTCTCC 6 0.15 No Hit GGGAGAGATTTGTGCATAGCAGGCAGGATTTTCTTAGCACTTGGTGATGA 6 0.15 No Hit CAGCAACTTCTTGTATTTCTTGCGAACTAAGTGACTGCCCACCAACAATG 6 0.15 No Hit CTCGGTACTTGGGCAACTCCAAAACTTCCTGCCTTTAGTCGGCCCATCTT 6 0.15 No Hit CTCACCAACCACTTCATGGCCAGGGACCATAGGATAGTGTGACATGCCAA 6 0.15 No Hit AGCGCCTTCTGGGATCTCGAATAAGCATTCTCCTCACTAAATCCTTAGCG 6 0.15 No Hit CCCCTGCAAGTTCTGGGTTGAAGCAGGGTCCTTCTGCAGAAGGCAACAGA 6 0.15 No Hit GCTCCAACTCTCTTTTCTCTTAAGACGTAGTCCTCCATAAATTATCATCT 6 0.15 No Hit CAAGGTACAAGAATGGCTTCTCAAAATTATAATTGCTCTTAGCTGAAATC 6 0.15 No Hit GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAACGCTTATCGCGTAT 6 0.15 TruSeq Adapter, Index 9 (97% over 37bp) GCTTTAGAAACCACCGAGTTCTGCCCAGAAATCTCGACCCCGTGTTTTCT 6 0.15 No Hit TCCTGGGCATTTATTTTGTCAGCAAGTTTACGAGCCACCAATAAGTTATA 6 0.15 No Hit CTCTGGTCAAAATCCTCACCTCCCAAATGAGTGTCACCATTGGTGGAGAG 6 0.15 No Hit CCTGGTGGTGGCGGCAGCATAACCACCGACTTCTTCTCATCCTCCTCGGC 6 0.15 No Hit GCTTCCCGACCCGAGCTTTCTCAGCTAGTTGCTTGACCTCTGAGGAGATT 6 0.15 No Hit CATGGATTGTATTTCTCCCTATTCATATGGACAAGTTTCTTTACCAAATT 6 0.15 No Hit GGGCATCAAGGTAATCCAAATCAAAGTTTCGATGCACAATGCGGCCCCCT 6 0.15 No Hit GTACACATGAGACTATGGAGATCGAATGCATGAGAATACAATTATTGCCA 6 0.15 No Hit GTATAACGCTACTTGCCCACTAGTGATCTTGCGTTCCCCAACAGAAGACT 6 0.15 No Hit AGCCTGACCATTATAACATTGGAAATACTACATTCCTTCAATGGATTCAA 6 0.15 No Hit CCTCAATGTTCACATCATGTAAAGTAGTTTCCTCACATTCATCAAGCTCC 6 0.15 No Hit GCCTACAAAAAAACTAGCTTGAACAAGTTGATCCTAATACTTAAAATAAT 6 0.15 No Hit CAGGTGCTGATGGCGCCAGTAAGGCTCATAAAATCTCCAAGGAAGGGCAG 5 0.125 No Hit GTAGAACTGGTCAAGGGTCTTAGCAGGTATACGGTGGAGTAGCTCACGGG 5 0.125 No Hit ACCCACACTACAAATTATGAAAATTCAAAACTAAATAACAATTACATTAA 5 0.125 No Hit CTGGTTGATAATCCAGTTACATATAAGTTGTTTCCAGGGTTAGATGCATC 5 0.125 No Hit GGGATGTTGTAATCAGCAAGGGTGCGGCCATCCTCAAGCTGCTTGCCGGC 5 0.125 No Hit AGCTCGTTTAGAAGGCAAGGATTCTACTCTTCCTCGGTGGCTGTCTCACC 5 0.125 No Hit ATGTGGATACCCTGGAGGAAGAAATATATCAAAAAAGAACAGTCCATCAT 5 0.125 No Hit ATGGAAAGCTAAAATGAAGGAAATAAAAAGGATTGCAGCAAGAGATGTAG 5 0.125 No Hit CCTATAAATAAAAACACAACTTCATTGCGTCATGTCATCGAATCTCAATA 5 0.125 No Hit GGCACCTATCAACCTCTCTGGCAACTCTTCTGTAGTATTTGCCTGTACAA 5 0.125 No Hit CTGGGTTTCATATTTGGTTTCGACCAATTGTAGGCGAATGAACCCGCAAT 5 0.125 No Hit ATGTGTTGGCCCGCTTGGGAGGAGATGACCCGTCAGTGTCTGGTGCACTC 5 0.125 No Hit GTCATTATGATAATACTGATCAAAATTATTAATCACAACATTCTTAATCC 5 0.125 No Hit ACTCCGACCACCGCTCTCCTTCCAATGGCCTAGCTGCCAATACTTGCTGA 5 0.125 No Hit ACGACATATGGATCCATGTTTGAAGCAGGCCTTCGGTCCTCAAAATACCC 5 0.125 No Hit GTCCCTCTCACGAACAAATTTATTCCAGCCCACAGAAAGAAAATGTCCCG 5 0.125 No Hit GTGGAGAATTGCTGTAGCTCCGGCTACGGCTGCGACGTCTATATCGACTA 5 0.125 No Hit CACTGCTCAGGTTACATGATCAAGCAACGGAGATTCTGTAACTGCTTATC 5 0.125 No Hit GGGAAGTTTGGAGTAAAAGTCAACGAGAGGTCCAAGCCCAGCACCCGATC 5 0.125 No Hit GCTTTATGTCTCTGTGGATGGTTGGCCTGGGAAATGCGGTGTGGAGATAT 5 0.125 No Hit CATCAGTCAAAACTTTGACAACCTAAAAATAATTCACAAGTCTTAACAGT 5 0.125 No Hit GTTCCGGTATCAACTTATTAACAAGCTCAGGAGAAGCCCCATTTAATTCC 5 0.125 No Hit CTCCAAAGTTCTCATAGTTGTTCACTAAGCTCAAAATCAGCTTAATCCCA 5 0.125 No Hit GGTGTTTCATCCATTGTAAGAAGATCCACAAAGTGCTGCGAGTACCTTCC 5 0.125 No Hit AGCAGCACCACCCATATAGTCAGGTCCAGTAAGTGCATAGTCAACAGGAG 5 0.125 No Hit GGCAGAGACAGATTTGGACGCAAGGACAAAGGCAGAAGCAGCAGCTTTTG 5 0.125 No Hit GTCCATTTCAAGCTTGTACACAGTTCCAAAACCACCAGAGCCTATGACAT 5 0.125 No Hit GTCCTTCTCATCGTGCCTCGTGCGACGAATCAAGGATCGACTTGTCTCTG 5 0.125 No Hit CTTTGTTAATAAATACAGATACACAGCAGAAAAGCCTCCACATCAGGAAC 5 0.125 No Hit GCTTGACTTCATTAACATGAAAATTTGACCCTCTAAACTCCAATGAAGTG 5 0.125 No Hit GTTGAATACATCAGTGTAGCGCGCGTGCGGCCCAGAACATCTAAGGGCAT 5 0.125 No Hit GCCCAGTCCCTTGAATCCCTCTAGGGGTAGCTCTGAAACAGTGACAAGGG 5 0.125 No Hit ACTATATATACCTGGGACTGCGAAGCAATAACTAGCATATTGGTTCTCTT 5 0.125 No Hit GCCACTGGACTTGTGTCTGCTTGCTGAGCTTCCCAGGGGGGTGGAGGCAG 5 0.125 No Hit GTTGAAATGTAATGCACAGATATATCTCAAAGCTCTCCATCCCTTTCTTT 5 0.125 No Hit GCCCTGATGACGATGATGGTTTTACCATTTTTTTAATTGTTTGCTTCTTT 5 0.125 No Hit CCTGCGTCGAAACACAACCTATTTGGCCCTGAGTGAGAAGAGAGTTGTGA 5 0.125 No Hit GGACACCATGACAACTCTCTTCACTTCCAATGGACAGGCGGGATCAAAAG 5 0.125 No Hit CGGGTCTTCTTTGATCAATCTCATTCAACTCTACAATTTAAAGTAACAAC 5 0.125 No Hit GCACGATCAAAACCCATAGCTTCAAGACGTTCTATTGCCTCGCGCTCCTC 5 0.125 No Hit CTTCACGTAAGGCTTAGTTCTCTTGTCAGTGGCTAGTTGGTGATCCACTA 5 0.125 No Hit CTCCACTTTGCCCTAAGAAAACCTGAATCATTCCTGGATGGTCTCTGGTG 5 0.125 No Hit GGAGCACTTTCACGGGCCATTTGGAAAAGGTTTGAAACAAGCTTTTCACT 5 0.125 No Hit GAACCCTCGGCAGCACCTGGGCCCTGCTTAAAGGCAGCTCCATTTTGAAA 5 0.125 No Hit ACTGGATCCGTCAAGAACCAAGACCATTGCATAAAATTAGGCGACTCATG 5 0.125 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.025 0.0 0.0 0.0 0.0 58-59 0.05 0.0 0.0 0.0 0.0 60-61 0.1 0.0 0.0 0.0 0.0 62-63 0.15 0.0 0.0 0.0 0.0 64-65 0.15 0.0 0.0 0.0 0.0 66-67 0.15 0.0 0.0 0.0 0.0 68-69 0.15 0.0 0.0 0.0 0.0 70-71 0.15 0.0 0.0 0.0 0.0 72-73 0.21250000000000002 0.0 0.0 0.0 0.0 74-75 0.2625 0.0 0.0 0.0 0.0 76-77 0.3 0.0 0.0 0.0 0.0 78-79 0.375 0.0 0.0 0.0 0.0 80-81 0.4375 0.0 0.0 0.0 0.0 82-83 0.475 0.0 0.0 0.0 0.0 84-85 0.475 0.0 0.0 0.0 0.0 86-87 0.5375 0.0 0.0 0.0 0.0 88-89 0.75 0.0 0.0 0.0 0.0 90-91 0.925 0.0 0.0 0.0 0.0 92-93 1.1 0.0 0.0 0.0 0.0 94-95 1.2875 0.0 0.0 0.0 0.0 96-97 1.5 0.0 0.0 0.0 0.0 98-99 1.7125 0.0 0.0 0.0 0.0 100-101 1.875 0.0 0.0 0.0 0.0 102-103 2.1125 0.0 0.0 0.0 0.0 104-105 2.4125 0.0 0.0 0.0 0.0 106-107 3.0999999999999996 0.0 0.0 0.0 0.0 108-109 3.55 0.0 0.0 0.0 0.0 110-111 4.0875 0.0 0.0 0.0 0.0 112-113 4.550000000000001 0.0 0.0 0.0 0.0 114-115 5.05 0.0 0.0 0.0 0.0 116-117 5.575 0.0 0.0 0.0 0.0 118-119 6.074999999999999 0.0 0.0 0.0 0.0 120-121 6.5625 0.0 0.0 0.0 0.0 122-123 7.525 0.0 0.0 0.0 0.0 124-125 8.3625 0.0 0.0 0.0 0.0 126-127 8.6625 0.0 0.0 0.0 0.0 128-129 9.3375 0.0 0.0 0.0 0.0 130-131 9.912500000000001 0.0 0.0 0.0 0.0 132-133 10.35 0.0 0.0 0.0 0.0 134-135 11.1375 0.0 0.0 0.0 0.0 136-137 11.925 0.0 0.0 0.0 0.0 138-139 12.6875 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content pass >>END_MODULE SRR26075365 read2 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR26075365_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.22825 37.0 37.0 37.0 37.0 37.0 2 36.3215 37.0 37.0 37.0 37.0 37.0 3 36.3645 37.0 37.0 37.0 37.0 37.0 4 36.2515 37.0 37.0 37.0 37.0 37.0 5 36.3155 37.0 37.0 37.0 37.0 37.0 6 36.311 37.0 37.0 37.0 37.0 37.0 7 36.1685 37.0 37.0 37.0 37.0 37.0 8 36.25 37.0 37.0 37.0 37.0 37.0 9 36.27 37.0 37.0 37.0 37.0 37.0 10-14 36.072300000000006 37.0 37.0 37.0 37.0 37.0 15-19 35.9268 37.0 37.0 37.0 37.0 37.0 20-24 35.867599999999996 37.0 37.0 37.0 37.0 37.0 25-29 35.730399999999996 37.0 37.0 37.0 37.0 37.0 30-34 35.569399999999995 37.0 37.0 37.0 37.0 37.0 35-39 35.458299999999994 37.0 37.0 37.0 37.0 37.0 40-44 35.4411 37.0 37.0 37.0 37.0 37.0 45-49 35.2833 37.0 37.0 37.0 37.0 37.0 50-54 35.1343 37.0 37.0 37.0 37.0 37.0 55-59 35.137899999999995 37.0 37.0 37.0 37.0 37.0 60-64 35.236000000000004 37.0 37.0 37.0 37.0 37.0 65-69 35.0869 37.0 37.0 37.0 34.6 37.0 70-74 35.11409999999999 37.0 37.0 37.0 34.6 37.0 75-79 34.9861 37.0 37.0 37.0 27.4 37.0 80-84 35.066100000000006 37.0 37.0 37.0 34.6 37.0 85-89 35.051 37.0 37.0 37.0 27.4 37.0 90-94 34.99390000000001 37.0 37.0 37.0 29.8 37.0 95-99 35.0835 37.0 37.0 37.0 32.2 37.0 100-104 34.9679 37.0 37.0 37.0 27.4 37.0 105-109 35.0227 37.0 37.0 37.0 29.8 37.0 110-114 34.99739999999999 37.0 37.0 37.0 27.4 37.0 115-119 34.9824 37.0 37.0 37.0 27.4 37.0 120-124 34.80135 37.0 37.0 37.0 25.0 37.0 125-129 34.7873 37.0 37.0 37.0 25.0 37.0 130-134 34.73479999999999 37.0 37.0 37.0 25.0 37.0 135-139 34.655100000000004 37.0 37.0 37.0 25.0 37.0 140-144 34.60785 37.0 37.0 37.0 25.0 37.0 145-149 34.42635 37.0 37.0 37.0 25.0 37.0 150-151 34.23725 37.0 37.0 37.0 25.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.0 1101 2 0.0 1101 3 0.0 1101 4 0.0 1101 5 0.0 1101 6 0.0 1101 7 0.0 1101 8 0.0 1101 9 0.0 1101 10-14 0.0 1101 15-19 0.0 1101 20-24 0.0 1101 25-29 0.0 1101 30-34 0.0 1101 35-39 0.0 1101 40-44 0.0 1101 45-49 0.0 1101 50-54 0.0 1101 55-59 0.0 1101 60-64 0.0 1101 65-69 0.0 1101 70-74 0.0 1101 75-79 0.0 1101 80-84 0.0 1101 85-89 0.0 1101 90-94 0.0 1101 95-99 0.0 1101 100-104 0.0 1101 105-109 0.0 1101 110-114 0.0 1101 115-119 0.0 1101 120-124 0.0 1101 125-129 0.0 1101 130-134 0.0 1101 135-139 0.0 1101 140-144 0.0 1101 145-149 0.0 1101 150-151 0.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 13 11.0 14 26.0 15 15.0 16 20.0 17 6.0 18 10.0 19 10.0 20 11.0 21 13.0 22 12.0 23 17.0 24 13.0 25 23.0 26 22.0 27 17.0 28 10.0 29 26.0 30 30.0 31 27.0 32 41.0 33 80.0 34 182.0 35 625.0 36 2540.0 37 213.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 47.23680920230057 22.1055263815954 9.827456864216053 20.830207551887973 2 32.324999999999996 23.275000000000002 27.375 17.025000000000002 3 26.275 26.450000000000003 28.975 18.3 4 27.55 30.85 22.625 18.975 5 30.4 32.625 20.3 16.675 6 25.275 37.175000000000004 20.8 16.75 7 25.974999999999998 22.175 34.75 17.1 8 29.475 22.15 25.275 23.1 9 25.074999999999996 25.05 27.35 22.525000000000002 10-14 27.26 28.249999999999996 24.779999999999998 19.71 15-19 27.42 27.310000000000002 25.5 19.77 20-24 26.33 28.405 25.555 19.71 25-29 27.084999999999997 27.405 26.445 19.064999999999998 30-34 27.310000000000002 27.6 25.61 19.48 35-39 26.055 28.22 25.240000000000002 20.485 40-44 27.165 27.02 25.555 20.26 45-49 26.645000000000003 26.575 26.334999999999997 20.445 50-54 24.375 27.73 28.625 19.27 55-59 26.31 28.225 25.979999999999997 19.485 60-64 25.869999999999997 27.589999999999996 26.5 20.04 65-69 26.72 27.51 26.0 19.77 70-74 25.765 29.4 25.105 19.73 75-79 25.935000000000002 29.39 26.334999999999997 18.34 80-84 25.569999999999997 28.725 25.779999999999998 19.925 85-89 25.35 29.225 25.21 20.215 90-94 26.229999999999997 28.075 26.35 19.345000000000002 95-99 25.605 28.455000000000002 26.035000000000004 19.905 100-104 25.509999999999998 29.385 25.56 19.545 105-109 26.240000000000002 27.97 25.915 19.875 110-114 26.245 28.470000000000002 26.305 18.98 115-119 26.290000000000003 28.294999999999998 26.44 18.975 120-124 26.666333316665835 28.001400070003502 25.90129506475324 19.43097154857743 125-129 26.02 29.134999999999998 26.415 18.43 130-134 26.965 28.665000000000003 25.55 18.82 135-139 26.430286057211443 28.585717143428685 25.975195039007804 19.00880176035207 140-144 27.211802950737685 29.457364341085274 25.376344086021508 17.95448862215554 145-149 27.551887971993 27.89697424356089 26.426606651662915 18.124531132783193 150-151 28.49462365591398 28.26956739184796 24.90622655663916 18.3295823955989 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.5 6 1.0 7 0.5 8 1.0 9 1.0 10 0.5 11 3.5 12 3.5 13 1.5 14 1.5 15 1.0 16 1.5 17 2.0 18 3.0 19 3.5 20 1.5 21 1.0 22 3.0 23 2.0 24 0.0 25 1.0 26 4.0 27 5.5 28 4.0 29 6.5 30 13.5 31 14.5 32 20.5 33 31.5 34 28.5 35 33.5 36 68.5 37 94.5 38 95.0 39 105.0 40 140.0 41 196.0 42 259.0 43 279.0 44 276.5 45 301.0 46 282.5 47 242.5 48 227.5 49 210.0 50 178.0 51 134.5 52 114.5 53 109.5 54 84.5 55 54.5 56 50.0 57 43.5 58 27.5 59 19.0 60 11.5 61 17.0 62 22.0 63 13.0 64 6.5 65 5.0 66 4.0 67 3.0 68 2.5 69 2.0 70 1.5 71 2.0 72 2.0 73 1.0 74 0.5 75 0.5 76 1.5 77 4.0 78 5.5 79 3.5 80 2.5 81 3.0 82 3.5 83 4.5 84 4.0 85 4.5 86 4.5 87 4.0 88 5.0 89 3.5 90 2.5 91 1.5 92 0.5 93 1.0 94 2.5 95 2.5 96 0.5 97 1.0 98 4.5 99 6.0 100 22.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.025 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.005 125-129 0.0 130-134 0.0 135-139 0.02 140-144 0.025 145-149 0.025 150-151 0.025 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 58.62500000000001 #Duplication Level Percentage of deduplicated Percentage of total 1 62.94243070362473 36.9 2 20.298507462686565 23.799999999999997 3 8.869936034115138 15.6 4 4.477611940298507 10.5 5 1.7484008528784647 5.125 6 0.9381663113006397 3.3000000000000003 7 0.4690831556503198 1.925 8 0.17057569296375266 0.8 9 0.0 0.0 >10 0.042643923240938165 0.5 >50 0.042643923240938165 1.55 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG 62 1.55 No Hit GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGT 20 0.5 No Hit CTTGACTGTTGATTGGGATAAATGGATTGATGAGGATGAGGAGTTCACTA 8 0.2 No Hit GTTTTATAGTTTAGTTAGACGGTAAAAGCGGCTAACAGGTACGCTTGGTT 8 0.2 No Hit GATGAATGCTGCCCACTACTGGAAGGTCTTGTAGACTTGGATGCTGCTGT 8 0.2 No Hit GTTCTACGAGAGGTCTGAGATGAACTCTGGTGCTGAGAATGGCTGCAAGT 8 0.2 No Hit TGATAGCAACAACAATTTGGAGAATTTAGCGAGAGATTTGGCATTGAGGC 7 0.17500000000000002 No Hit CAGCACCGGAGGTGAGGCCGAAGCTAACGAAGGTATTAGTAAACGTTACG 7 0.17500000000000002 No Hit GTGAAAACCCTGACTGGCAAGACCATCACTCTTGAGGTCGAATCTAGTGA 7 0.17500000000000002 No Hit TGGACTTCCTTGAACAGTTAAGTTTTCATGTCTGATTTCGACGCCCAAAC 7 0.17500000000000002 No Hit GCCAAGAGTTATCTTTTGAAACTGCTAGTATGTCTCCCCGAGAGACCTGG 7 0.17500000000000002 No Hit TGACAAGATAAAGGATGAGTATGTTAAAGCTTATTATGCTGCTCTGCTTC 7 0.17500000000000002 No Hit TATTTGTGGCTTCTGACCAATTTGGTAAGGTTCCTCTGTATTGGGGAATC 7 0.17500000000000002 No Hit TTTTGGTTACGCTAAGTTTTCGATGCAAGGAAAGACTTCTTTATCTGGCA 7 0.17500000000000002 No Hit TGGAATGCTATTAATGGCTGACAAGTGTGAGAACATGGAAAGTATTTGTC 7 0.17500000000000002 No Hit TGATGTGGAAATTGCACCCCTTGATTCATTATAACACATCCTGTCTAAAG 7 0.17500000000000002 No Hit GTGAATTGGGTTTTTTTTTTTGGTTTTTAGGTCACTGGCTTATTGATGTT 7 0.17500000000000002 No Hit AGTACCCTAACGGATCGAGACCGAATCGTGCTGCAGGGAGGGGCTACTGG 6 0.15 No Hit TCTAAGTGCTGATGAGCTTCACAGCTTACTGTCTATACGAGACTAATAAT 6 0.15 No Hit AATCTCCCGTTTACACCGAGAGCAAAAGCAAAAGAAGAAAGGAAAACCGT 6 0.15 No Hit GGAATCAAAACAGACGACACCACATTTATATTTATCCACAGATGTTATTC 6 0.15 No Hit GGGAGGGGGATTTGTGAAGCTGTTGATTCTAAAGCACCAAGGAACTGGGA 6 0.15 No Hit GTTACAAAGAAGAAAAAGAAGAAAAATAAAAGCAAGAAAAAGAAGGAATC 6 0.15 No Hit CTGTCCTGTTGGAATTCTCTTCTGGATGCAAATGGCCAGTCTCCATATGC 6 0.15 No Hit GGTTGAAGCTTGTCATTCACTGGGTGTGATGCATCGAGATCTTAAACCAG 6 0.15 No Hit ACTTCTTCCAATGGGCTGATGCTCTGCCGAGTCAATCAGCACCTAGCCAC 6 0.15 No Hit TAAAAAAATGGTAAAACCATCATCGTCATCAGGGCAACAGTCTGATAGAA 6 0.15 No Hit GTTTGCATATTTCAGCCATACATATCAAGTTGAGGAAACAATTTGTCTTT 6 0.15 No Hit CCTTTTTGTGCGAGTTGTGAAGGCTCGTGACCTTCCTACCATGGATGTGA 6 0.15 No Hit CGTGGGTGGGCTCTGCTCTTTGATAAAGTTCATCTCAGAGATTCTTACCA 6 0.15 No Hit CTTCGTGGAACAGTGCGATTACATATTAAGCCACCTCCTTCTGATCAGTT 6 0.15 No Hit TGTCTCATGGAGATCGTCTTCTCTCTCTTGCCTCTACTTCTTTCGGTGAC 6 0.15 No Hit TTGCTGGACTCAATGTGGCTAGAATTATTAACGAGCCAACCGCCGCTGCT 6 0.15 No Hit GCATTATGTGAAAGAAACCAATGATGCATTTTATCACCAGCCAAATGCAT 6 0.15 No Hit ATTCCAATTGTTCTTTGTGGAAACAAGGTGGATGTGAAGAACAGGCAGGT 6 0.15 No Hit GGGAAACTGTACATTCATTTCACTGTTGATTTCCCAGACTCCCTGTCCCT 6 0.15 No Hit CTCCATCTCTCTCTCTTAGCCTCATTGTTTCAAGAAAATGGGTAGCCTTG 6 0.15 No Hit GCTCAAGTTCCACAAAAGATCAAACAAGCTTCCAGCAATAGTGGCAGAAA 6 0.15 No Hit AGGAAGTTTGCTCAGAAGGCCATGGGGACTACTGATGTTAGAGTTGATGT 6 0.15 No Hit GCTTTATGTTCTCAAGCTTTCAGGTTGCTGTTTGGAGATCCCAGCAGGTC 5 0.125 No Hit GGAGACTCACCGGAAGGCACGAAACAGCTGACATTAACACTTTCAAATGG 5 0.125 No Hit GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGT 5 0.125 No Hit GCTGAAACGGCCAAGAAACGAGAACAGGAGCGCCAGAGGCAGGCGAAACT 5 0.125 No Hit GCAGACTGCCAGGAAACTACAGAGAAATTCCAGGAAATGGACTTGTTATG 5 0.125 No Hit AATTTGGACATGGAAATGGAACTAATGTGGTTTGGAGCTTGGAAGTGGCA 5 0.125 No Hit GTTGATATTGGTTCTTCTACTGTTGCAAATGGAGTCTTGGGACTTGTAAT 5 0.125 No Hit GCGAGGCTGTTCAATGTGCTCATAGTCAAATCCGATTGGGTTTCCCCGAC 5 0.125 No Hit CTTGGTTTAACACACAAAATACCAGGTGTTGTTGAGTCATTGGTCAACGA 5 0.125 No Hit CGTTACCTATCCTCCGTGCTTTTCGAATCGGTGAAGAAGGAGGCGGAGAA 5 0.125 No Hit GTTTTTGTTGTTAAAAAGAAATAATGTTAAATCTACGATGATAATTTTTA 5 0.125 No Hit CAGGAACAGGGAGCCCCCACAGCGTTTCATTAGACGCAGGGATGACATGC 5 0.125 No Hit AGGGGAAGAAGACATTCCAGATCCCCTGGTTTTTTGGAGTTTCCAGCTGT 5 0.125 No Hit AATGCCCCGAATACGGGATCACCACGATATGAGCAGTCAGTATACATGCA 5 0.125 No Hit CTACTTGAAGGATAAAGTGCAACCATCTTTTGTCAAAGATCGTAGAGCTA 5 0.125 No Hit GGGAAGCACAGCATTGGATGCCTATTTTGGCTTTTGGCAAGGATGGTTCT 5 0.125 No Hit AGGTGCCAAGGTTTGCACCAGAGCTTGATGGACTCAACTGCTTCGAGACC 5 0.125 No Hit GTGAAGCTGGTGCACCGTTTGTACCCGGAAGTTGACCCAGCACTAAACCC 5 0.125 No Hit CAATTGTCTCTCTCTCCCAGACACAACAAGATTCTCTCGTCAAAATGGCT 5 0.125 No Hit GTTTCTTTGGGTCTAATTTTCAGGGGATATATACCGTCGGTGTGCAAGCA 5 0.125 No Hit TTATGTTGACAGGCAGCTACAAAATAGGCAGATTTATCCTCCTATCAACG 5 0.125 No Hit GCTAGATACTAGTAGTAGTTGCAGACAGAGAGCTAGTGTTAGACAGGTTC 5 0.125 No Hit CACCAACTCTTCGTCTCAGAGATGGCCAAGTCAAAGAATCACACAGCACA 5 0.125 No Hit AGTATTTGGATACGAAGTTTGGGGTTAAAGGAAGTCCACTTGCAATTCGC 5 0.125 No Hit ACCTAGCCTTCTTCGTCCTCAAGAACACCCGTCTAGCTAGCTCTTTACAC 5 0.125 No Hit TGCTCGTCGAGGTGACACACTTGGAGGGGGATGATGACAAGAACGATCAG 5 0.125 No Hit ATTCGAGACATGAGACCTGGGGGGAAGAGAAAGATAATCATTCCTCAAGA 5 0.125 No Hit CAGGCAGCAAAGCTGGATTTGATAGACCCATTTTTGTCTACCACAAATGA 5 0.125 No Hit CATCCCTGCTACAAAAGTAGAAGCAGCTCCAACCACTGATGTTGGAAAGG 5 0.125 No Hit AAATCTTTGTTAAGACCCTTACTGGAAAGACCATCACCTTGGAGGTGGAG 5 0.125 No Hit ATTTGTCTGGTTAATTCCGTTAACGAACGAGACCTCAGCCTGCTAACTAG 5 0.125 No Hit GTGGCGGGTCTGGAACTGATGGGCAGCCTCCTTCAGGCCAAAATCAAGGA 5 0.125 No Hit GTTCCCTCAGTTTTTTACAGGTAAGAGACGACCTTGGAGGGCTTTTCTTT 5 0.125 No Hit ACCCAATGCTCGCCTGCCAAATGGCCGCCCTTTACCATCTCTCTTCAACT 5 0.125 No Hit CTCCGAACGTCGTCCAACACAGTCACTATGCACGCCTCCCTTATCCGCCG 5 0.125 No Hit AGATGACGGGTTCATAAGAACCAAAGGAGTGCAATTCTTGTTGAATGGGA 5 0.125 No Hit GCTTGCTGCAGCAATGCCACAAGCTGTAACAGTCACTCCTGAGGAGCGCG 5 0.125 No Hit GAGAATCTAAGCTGGGCCAAGGAGAAAGCTAAAGAGAATTATGAGACTGC 5 0.125 No Hit ATTACAGCGTTGAGTTTCACTTTCCTGAACCTACTGAGCTTACACCACCA 5 0.125 No Hit CCTGTGTGTAAGTTCATCCGAGTTGACTCAGCCAATAACTCAGATGGCTT 5 0.125 No Hit CTTCAGTTCCTACTACTACTTCACCAGCAAAGATTGTTGGTGGGCTTTTC 5 0.125 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.025 0.0 0.0 0.0 0.0 58-59 0.05 0.0 0.0 0.0 0.0 60-61 0.1 0.0 0.0 0.0 0.0 62-63 0.15 0.0 0.0 0.0 0.0 64-65 0.15 0.0 0.0 0.0 0.0 66-67 0.15 0.0 0.0 0.0 0.0 68-69 0.15 0.0 0.0 0.0 0.0 70-71 0.15 0.0 0.0 0.0 0.0 72-73 0.21250000000000002 0.0 0.0 0.0 0.0 74-75 0.2625 0.0 0.0 0.0 0.0 76-77 0.3 0.0 0.0 0.0 0.0 78-79 0.375 0.0 0.0 0.0 0.0 80-81 0.42500000000000004 0.0 0.0 0.0 0.0 82-83 0.45 0.0 0.0 0.0 0.0 84-85 0.45 0.0 0.0 0.0 0.0 86-87 0.5125 0.0 0.0 0.0 0.0 88-89 0.7250000000000001 0.0 0.0 0.0 0.0 90-91 0.8999999999999999 0.0 0.0 0.0 0.0 92-93 1.075 0.0 0.0 0.0 0.0 94-95 1.225 0.0 0.0 0.0 0.0 96-97 1.4125 0.0 0.0 0.0 0.0 98-99 1.6124999999999998 0.0 0.0 0.0 0.0 100-101 1.775 0.0 0.0 0.0 0.0 102-103 2.0125 0.0 0.0 0.0 0.0 104-105 2.3125 0.0 0.0 0.0 0.0 106-107 3.0 0.0 0.0 0.0 0.0 108-109 3.55 0.0 0.0 0.0 0.0 110-111 4.0875 0.0 0.0 0.0 0.0 112-113 4.550000000000001 0.0 0.0 0.0 0.0 114-115 5.025 0.0 0.0 0.0 0.0 116-117 5.575 0.0 0.0 0.0 0.0 118-119 6.0875 0.0 0.0 0.0 0.0 120-121 6.5875 0.0 0.0 0.0 0.0 122-123 7.550000000000001 0.0 0.0 0.0 0.0 124-125 8.4 0.0 0.0 0.0 0.0 126-127 8.7375 0.0 0.0 0.0 0.0 128-129 9.399999999999999 0.0 0.0 0.0 0.0 130-131 9.9875 0.0 0.0 0.0 0.0 132-133 10.425 0.0 0.0 0.0 0.0 134-135 11.2125 0.0 0.0 0.0 0.0 136-137 12.0 0.0 0.0 0.0 0.0 138-139 12.7625 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGAATTT 10 0.006830828 145.0 3 >>END_MODULE Read 1001116 spots for SRR26075365.sra Written 1001116 spots for SRR26075365.sra Read 1001116 spots for SRR26075365.sra Written 1001116 spots for SRR26075365.sra Read 1001116 spots for SRR26075365.sra Written 1001116 spots for SRR26075365.sra Read 1001116 spots for SRR26075365.sra Written 1001116 spots for SRR26075365.sra Read 1001116 spots for SRR26075365.sra Written 1001116 spots for SRR26075365.sra Read 1001116 spots for SRR26075365.sra Written 1001116 spots for SRR26075365.sra Read 1001116 spots for SRR26075365.sra Written 1001116 spots for SRR26075365.sra Read 1001116 spots for SRR26075365.sra Written 1001116 spots for SRR26075365.sra Read 1001116 spots for SRR26075365.sra Written 1001116 spots for SRR26075365.sra Read 1001116 spots for SRR26075365.sra Written 1001116 spots for SRR26075365.sra Read 1001116 spots for SRR26075365.sra Written 1001116 spots for SRR26075365.sra Read 1001120 spots for SRR26075365.sra Written 1001120 spots for SRR26075365.sra Read 1001116 spots for SRR26075365.sra Written 1001116 spots for SRR26075365.sra Read 1001116 spots for SRR26075365.sra Written 1001116 spots for SRR26075365.sra Read 1001116 spots for SRR26075365.sra Written 1001116 spots for SRR26075365.sra Read 1001116 spots for SRR26075365.sra Written 1001116 spots for SRR26075365.sra Read 1001116 spots for SRR26075365.sra Written 1001116 spots for SRR26075365.sra Read 1001116 spots for SRR26075365.sra Written 1001116 spots for SRR26075365.sra Read 1001116 spots for SRR26075365.sra Written 1001116 spots for SRR26075365.sra Read 1001116 spots for SRR26075365.sra Written 1001116 spots for SRR26075365.sra SRR ids: ['SRR26075365.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_k_rnkz1q SRR26075365.sra spots: 20022324 blocks: [[1, 1001116], [1001117, 2002232], [2002233, 3003348], [3003349, 4004464], [4004465, 5005580], [5005581, 6006696], [6006697, 7007812], [7007813, 8008928], [8008929, 9010044], [9010045, 10011160], [10011161, 11012276], [11012277, 12013392], [12013393, 13014508], [13014509, 14015624], [14015625, 15016740], [15016741, 16017856], [16017857, 17018972], [17018973, 18020088], [18020089, 19021204], [19021205, 20022324]] SRR26075365 file size 7389308 SRR26075365 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o SRR26075365 SRR26075365_1.fastq SRR26075365_2.fastq Input file: SRR26075365_1.fastq Paired file: SRR26075365_2.fastq trimmed: SRR26075365-trimmed-pair1.fastq, SRR26075365-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Tue Feb 11 23:20:39 2025 >> started Tue Feb 11 23:21:05 2025 >> done (25.302s) 20022324 read pairs processed; of these: 107 ( 0.00%) short read pairs filtered out after trimming by size control 172479 ( 0.86%) empty read pairs filtered out after trimming by size control 19849738 (99.14%) read pairs available; of these: 3223490 (16.24%) trimmed read pairs available after processing 16626248 (83.76%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 18 3 0.00% 19 11 0.00% 20 4 0.00% 21 9 0.00% 22 8 0.00% 23 17 0.00% 24 19 0.00% 25 16 0.00% 26 36 0.00% 27 19 0.00% 28 43 0.00% 29 42 0.00% 30 37 0.00% 31 35 0.00% 32 51 0.00% 33 28 0.00% 34 33 0.00% 35 33 0.00% 36 42 0.00% 37 50 0.00% 38 72 0.00% 39 74 0.00% 40 71 0.00% 41 71 0.00% 42 112 0.00% 43 70 0.00% 44 113 0.00% 45 86 0.00% 46 128 0.00% 47 106 0.00% 48 131 0.00% 49 135 0.00% 50 171 0.00% 51 155 0.00% 52 227 0.00% 53 180 0.00% 54 246 0.00% 55 255 0.00% 56 327 0.00% 57 363 0.00% 58 442 0.00% 59 407 0.00% 60 493 0.00% 61 511 0.00% 62 611 0.00% 63 647 0.00% 64 825 0.00% 65 776 0.00% 66 971 0.00% 67 940 0.00% 68 1137 0.01% 69 1357 0.01% 70 1402 0.01% 71 1786 0.01% 72 1868 0.01% 73 2286 0.01% 74 2502 0.01% 75 2851 0.01% 76 3247 0.02% 77 3439 0.02% 78 3973 0.02% 79 4081 0.02% 80 4685 0.02% 81 5382 0.03% 82 5881 0.03% 83 6600 0.03% 84 7842 0.04% 85 8319 0.04% 86 9014 0.05% 87 9618 0.05% 88 10541 0.05% 89 11346 0.06% 90 12411 0.06% 91 13256 0.07% 92 14626 0.07% 93 15877 0.08% 94 17480 0.09% 95 18674 0.09% 96 19606 0.10% 97 20671 0.10% 98 21697 0.11% 99 22663 0.11% 100 23934 0.12% 101 25160 0.13% 102 26778 0.13% 103 28426 0.14% 104 29872 0.15% 105 31606 0.16% 106 33183 0.17% 107 33882 0.17% 108 35157 0.18% 109 36599 0.18% 110 37229 0.19% 111 38255 0.19% 112 40085 0.20% 113 41689 0.21% 114 43013 0.22% 115 45020 0.23% 116 46225 0.23% 117 48204 0.24% 118 48653 0.25% 119 48868 0.25% 120 51202 0.26% 121 51598 0.26% 122 52609 0.27% 123 55196 0.28% 124 57207 0.29% 125 56981 0.29% 126 60317 0.30% 127 60827 0.31% 128 61831 0.31% 129 63797 0.32% 130 65074 0.33% 131 65730 0.33% 132 66864 0.34% 133 67600 0.34% 134 70121 0.35% 135 71693 0.36% 136 72522 0.37% 137 74187 0.37% 138 74310 0.37% 139 75841 0.38% 140 76537 0.39% 141 77523 0.39% 142 78554 0.40% 143 79961 0.40% 144 82611 0.42% 145 83207 0.42% 146 84165 0.42% 147 86165 0.43% 148 86742 0.44% 149 86208 0.43% 150 88102 0.44% 151 16626248 83.76% 19849738 reads passed initial QC criterion=sequence-density sequence-density=0.26 sequence-density-rank=1 fanout-score=22.90 fanout-score-rank=5 prefix-density=0.23 prefix-fanout=22.9 sequence=GGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAACGCTTATCTCGTATGCCGTCTTCTGCTTGAAAA criterion=fanout-score sequence-density=0.03 sequence-density-rank=35 fanout-score=136.59 fanout-score-rank=1 prefix-density=0.34 prefix-fanout=13.1 sequence=CATCACCATCCCTGATTGATCTTTGATTCACAACAAGACCAACCAACCGTACAATGTTTACATAACTTGGGATAATCTCACAAGAAAGAAAGATCAATACAAAACTAGTCAGCTCAGGATGTTCCAGGCACGGTAGAACCGTAGAACCATAACAACAAGAGACATATTGCAGATGAGTACTGAAAAACAAAACACAGTACGTATTTACATGGGCAACCTTGGTTGAAGGCAACCTCATCAACGATGCTCGCTCTTCGTCTCTCCACTGTACATCCAGTCATAGACAGTGGGTGTGTTAGGCTGTGGCTTGTCAAAAACATGAGCACCAATATTCTTAGTAGCAAGGTTGCTACCAGGGTGGAACACGCTCCTCCAAACATTGCTACGCGCCGACACTGGGGTTGTAGGGGTCACTGGTGTCGTCGGTGTCCCTGGAGTTCCTGGCATAGTCATGGACCTCTGAAACTTATTAACAGGACTGCTCCCCTCTCCGACGTCAATATCTTTGATG criterion=sequence-density sequence-density=0.29 sequence-density-rank=1 fanout-score=2.59 fanout-score-rank=36 prefix-density=0.29 prefix-fanout=2.5 sequence=ATGTACCCTGACTTAGGTTTCTCAGAGA criterion=fanout-score sequence-density=0.09 sequence-density-rank=23 fanout-score=376.51 fanout-score-rank=1 prefix-density=1.08 prefix-fanout=32.1 sequence=AAGAAGAAGAAA SRR26075365 testing PE reads STAR mapping to Ensembl genome Started job on | Feb 11 23:21:46 Started mapping on | Feb 11 23:21:46 Finished on | Feb 11 23:25:07 Mapping speed, Million of reads per hour | 355.52 Number of input reads | 19849738 Average input read length | 293 UNIQUE READS: Uniquely mapped reads number | 17557019 Uniquely mapped reads % | 88.45% Average mapped length | 292.08 Number of splices: Total | 15142701 Number of splices: Annotated (sjdb) | 14717966 Number of splices: GT/AG | 14862783 Number of splices: GC/AG | 205417 Number of splices: AT/AC | 17518 Number of splices: Non-canonical | 56983 Mismatch rate per base, % | 0.40% Deletion rate per base | 0.04% Deletion average length | 3.51 Insertion rate per base | 0.02% Insertion average length | 2.61 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 533728 % of reads mapped to multiple loci | 2.69% Number of reads mapped to too many loci | 55820 % of reads mapped to too many loci | 0.28% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 7.97% % of reads unmapped: other | 0.61% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 1758991 1758991 1758991 N_multimapping 533728 533728 533728 N_noFeature 493004 17332313 624413 N_ambiguous 204155 1467 110005 UnstrandedReadsAssigned:16859860 PositiveStrandReadsAssigned:223239 NegativeStrandReadsAssigned:16822601 Dataset is classified negative stranded MeadianReadLen=151 20thPercentileLength=151 echo kmer=147 SRR26075365 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,400 [index] number of k-mers: 62,057,036 [index] number of equivalence classes: 130,681 [quant] running in paired-end mode [quant] will process pair 1: SRR26075365-trimmed-pair1.fastq SRR26075365-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 19,849,738 reads, 17,243,777 reads pseudoaligned [quant] estimated average fragment length: 210.33 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,162 rounds 52401 SRR26075365.ke.tsv 34699 SRR26075365.se.tsv 87100 total ==> SRR26075365.ke.tsv <== target_id length eff_length est_counts tpm Potri.005G200100.1.v4.1 2018 1808.67 2536 63.4743 Potri.005G024800.1.v4.1 1035 825.67 7439 407.865 Potri.004G059700.1.v4.1 961 751.67 7 0.421579 Potri.007G009000.2.v4.1 1416 1206.67 0 0 Potri.003G141000.2.v4.1 2943 2733.67 788 13.0493 Potri.016G087400.1.v4.1 270 90.2473 1932 969.128 Potri.015G069301.1.v4.1 564 356.199 0 0 Potri.010G195200.1.v4.1 1773 1563.67 182 5.26907 Potri.012G127500.1.v4.1 977 767.67 10084 594.656 ==> SRR26075365.se.tsv <== Potri.001G166300.v4.1 0 Potri.001G448400.v4.1 137 Potri.001G233950.v4.1 0 Potri.001G122700.v4.1 270 Potri.001G212900.v4.1 0 Potri.001G182400.v4.1 0 Potri.001G256600.v4.1 0 Potri.001G040500.v4.1 68 Potri.001G416900.v4.1 0 Potri.001G452600.v4.1 410 SRR26075365 completed mapping pipeline successfully