Starting /dee2/code/volunteer_pipeline.sh SRR26075366 current disk space = 3052537364480 free memory = 1465551956 SRR26075366 SRAfilesize 3dd96ef49d10dda723b9ad3dde587380 SRR26075366.sra SRR26075366.sra file validated SRR26075366 is paired end SRR26075366 is conventional basespace SRR26075366 read1 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR26075366_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.64125 37.0 37.0 37.0 37.0 37.0 2 36.475 37.0 37.0 37.0 37.0 37.0 3 36.579 37.0 37.0 37.0 37.0 37.0 4 36.6785 37.0 37.0 37.0 37.0 37.0 5 36.6955 37.0 37.0 37.0 37.0 37.0 6 36.6105 37.0 37.0 37.0 37.0 37.0 7 36.5685 37.0 37.0 37.0 37.0 37.0 8 36.7125 37.0 37.0 37.0 37.0 37.0 9 36.7755 37.0 37.0 37.0 37.0 37.0 10-14 36.654 37.0 37.0 37.0 37.0 37.0 15-19 36.556799999999996 37.0 37.0 37.0 37.0 37.0 20-24 36.5363 37.0 37.0 37.0 37.0 37.0 25-29 36.433099999999996 37.0 37.0 37.0 37.0 37.0 30-34 36.404399999999995 37.0 37.0 37.0 37.0 37.0 35-39 36.359300000000005 37.0 37.0 37.0 37.0 37.0 40-44 36.2768 37.0 37.0 37.0 37.0 37.0 45-49 36.248400000000004 37.0 37.0 37.0 37.0 37.0 50-54 36.2577 37.0 37.0 37.0 37.0 37.0 55-59 36.1546 37.0 37.0 37.0 37.0 37.0 60-64 36.1373 37.0 37.0 37.0 37.0 37.0 65-69 36.0796 37.0 37.0 37.0 37.0 37.0 70-74 36.0471 37.0 37.0 37.0 37.0 37.0 75-79 35.974900000000005 37.0 37.0 37.0 37.0 37.0 80-84 35.9665 37.0 37.0 37.0 37.0 37.0 85-89 35.9075 37.0 37.0 37.0 37.0 37.0 90-94 35.7725 37.0 37.0 37.0 37.0 37.0 95-99 35.8069 37.0 37.0 37.0 37.0 37.0 100-104 35.838800000000006 37.0 37.0 37.0 37.0 37.0 105-109 35.7254 37.0 37.0 37.0 37.0 37.0 110-114 35.636900000000004 37.0 37.0 37.0 37.0 37.0 115-119 35.565 37.0 37.0 37.0 37.0 37.0 120-124 35.531 37.0 37.0 37.0 37.0 37.0 125-129 35.480500000000006 37.0 37.0 37.0 37.0 37.0 130-134 35.349599999999995 37.0 37.0 37.0 34.6 37.0 135-139 35.2599 37.0 37.0 37.0 29.8 37.0 140-144 35.126099999999994 37.0 37.0 37.0 29.8 37.0 145-149 35.0526 37.0 37.0 37.0 25.0 37.0 150-151 34.894999999999996 37.0 37.0 37.0 25.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.0 1101 2 0.0 1101 3 0.0 1101 4 0.0 1101 5 0.0 1101 6 0.0 1101 7 0.0 1101 8 0.0 1101 9 0.0 1101 10-14 0.0 1101 15-19 0.0 1101 20-24 0.0 1101 25-29 0.0 1101 30-34 0.0 1101 35-39 0.0 1101 40-44 0.0 1101 45-49 0.0 1101 50-54 0.0 1101 55-59 0.0 1101 60-64 0.0 1101 65-69 0.0 1101 70-74 0.0 1101 75-79 0.0 1101 80-84 0.0 1101 85-89 0.0 1101 90-94 0.0 1101 95-99 0.0 1101 100-104 0.0 1101 105-109 0.0 1101 110-114 0.0 1101 115-119 0.0 1101 120-124 0.0 1101 125-129 0.0 1101 130-134 0.0 1101 135-139 0.0 1101 140-144 0.0 1101 145-149 0.0 1101 150-151 0.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 17 1.0 18 0.0 19 0.0 20 0.0 21 5.0 22 1.0 23 1.0 24 5.0 25 13.0 26 12.0 27 15.0 28 26.0 29 20.0 30 30.0 31 34.0 32 62.0 33 86.0 34 175.0 35 472.0 36 2850.0 37 192.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 46.860145108831624 12.534400800600451 7.080310232674505 33.52514385789342 2 18.35 13.65 35.525 32.475 3 16.875 20.375 29.675 33.074999999999996 4 21.925 25.474999999999998 25.650000000000002 26.950000000000003 5 22.400000000000002 33.7 25.025 18.875 6 21.125 32.875 25.7 20.3 7 13.850000000000001 26.8 40.6 18.75 8 18.224999999999998 26.075 33.4 22.3 9 17.325 23.150000000000002 35.199999999999996 24.325 10-14 19.845 30.595 26.669999999999998 22.89 15-19 19.7 28.13 28.725 23.445 20-24 20.825 28.77 27.215 23.189999999999998 25-29 20.095 28.015 27.27 24.62 30-34 20.215 28.21 27.839999999999996 23.735 35-39 20.255000000000003 27.755000000000003 28.03 23.96 40-44 20.48 27.93 27.415 24.175 45-49 20.599999999999998 27.76 28.439999999999998 23.200000000000003 50-54 20.200000000000003 28.645 26.235000000000003 24.92 55-59 20.075000000000003 27.589999999999996 28.299999999999997 24.035 60-64 19.605 29.044999999999998 27.38 23.97 65-69 20.02 28.325 27.785 23.87 70-74 20.085 28.9 27.43 23.585 75-79 20.549999999999997 26.985 27.41 25.055 80-84 20.985 27.33 27.51 24.175 85-89 20.785 27.765 27.0 24.45 90-94 19.73 28.345 28.189999999999998 23.735 95-99 20.22 27.925 27.785 24.07 100-104 20.435 28.09 27.07 24.404999999999998 105-109 20.005 28.634999999999998 27.83 23.53 110-114 21.47 28.1 27.384999999999998 23.044999999999998 115-119 21.38 27.36 27.68 23.580000000000002 120-124 21.62 28.225 26.43 23.724999999999998 125-129 21.795 27.48 27.125 23.599999999999998 130-134 20.895 28.82 26.965 23.32 135-139 21.615000000000002 28.105000000000004 26.974999999999998 23.305 140-144 21.82 27.63 27.115000000000002 23.435 145-149 21.95 27.779999999999998 26.405 23.865 150-151 21.2 27.6375 27.5625 23.599999999999998 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.5 4 0.5 5 0.0 6 0.0 7 0.0 8 0.5 9 0.5 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.5 20 1.0 21 1.0 22 1.5 23 1.5 24 2.0 25 4.5 26 5.0 27 3.0 28 5.0 29 14.0 30 20.0 31 16.0 32 15.5 33 32.0 34 43.0 35 71.0 36 105.5 37 109.5 38 121.0 39 151.0 40 189.5 41 220.5 42 250.0 43 249.0 44 252.5 45 261.0 46 253.0 47 257.0 48 237.0 49 190.0 50 159.0 51 161.0 52 149.0 53 113.0 54 88.0 55 68.5 56 50.0 57 40.5 58 21.0 59 11.5 60 10.0 61 6.5 62 4.5 63 5.0 64 9.0 65 6.5 66 2.5 67 1.5 68 1.0 69 0.0 70 0.5 71 0.5 72 0.0 73 2.0 74 3.0 75 1.0 76 0.0 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.075 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150-151 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 56.699999999999996 #Duplication Level Percentage of deduplicated Percentage of total 1 60.18518518518518 34.125 2 21.428571428571427 24.3 3 9.435626102292769 16.05 4 4.320987654320987 9.8 5 2.513227513227513 7.124999999999999 6 1.0582010582010581 3.5999999999999996 7 0.5291005291005291 2.1 8 0.26455026455026454 1.2 9 0.08818342151675485 0.44999999999999996 >10 0.1763668430335097 1.25 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GGAGAAAGAAAGAAAAATGGGAAGGGAAAGAAATGAGAGAAACCATGCTA 15 0.375 No Hit CAGCAGCCACAGTCAGAACCACGGCCAGGGCGGTGGTACTGTTGTGTAGG 14 0.35000000000000003 No Hit CATCTTCTTTGGTCTCCCTGAAGGGACCCTTATTCCTGCTTTTACTTGAA 11 0.27499999999999997 No Hit CACCATCTCCGAGACTGCGATACCCTTCGGATTTGATGGAAGATTGGTGG 10 0.25 No Hit CCAGGTTTTTCGACCTCTCTGACAATGTGGGTCATGCCAGCCTTGTAACC 9 0.22499999999999998 No Hit GTTAATTTTCCCGTCTTTGTTTTTGTCCATTTCATTGAAAGAAAATCTGG 9 0.22499999999999998 No Hit CTCAGCCTCTCAGCGTATACCTCACTTCCTTGGTATTCGTACCTTTGCGG 8 0.2 No Hit CATGAATTCCCGATGCTTTGGTGAATACCAAGCAACAGCCTTTGCTGAAC 8 0.2 No Hit GCTGCTGCTGCTATTGTTGTCTTAAGGCTGCTGCTGCTATTGTTGCTTAA 8 0.2 No Hit GCATATATAAAATATAAAAACATCTCCATTGTTATTCTTTGAAAATTATC 8 0.2 No Hit GCACTTGTTGTTACTCTCAGCAACAGAAGGCCCGTTCAAACGAATCTTCA 8 0.2 No Hit GCCGGGTTCGATACTGGGCAGGATGAAGCCATGACTCAAAAACACCATGT 8 0.2 No Hit CCCCTTAAAAGCAAAGCCATCTCTTTCAAACTTCGGATATTTGCTTTTCT 7 0.17500000000000002 No Hit GTCACCTCTTGCTGGTTGTTGAGTTTAGCAACATCAATGGGCGTCTTGCC 7 0.17500000000000002 No Hit TCCGCACTTGCAGCCATTCTCAGCACCAGAGTTCATCTCAGACCTCTCGA 7 0.17500000000000002 No Hit CCCTTCCTCCTTCATTGTTCGCTTAAAACAATCACCAATGCCCTTGTAGG 7 0.17500000000000002 No Hit AACAGGAGGGAGTGGTTAATAACAGCAGATCTAAACAATCTACTATGGAC 7 0.17500000000000002 No Hit GTACTGTTGAGAACATAATTTTCACTCCTACACCGTTGCAACTGTTCTTG 7 0.17500000000000002 No Hit ATCGTAATTCAACATCCCCCTCGGATTACTACTCTCTGAATCCCCCAGAA 7 0.17500000000000002 No Hit ACTTCATCCAGACCTACAGAAGAACAAAATAATTAAAGATGCCTTACACT 7 0.17500000000000002 No Hit GCGTATCAAAGACGAAACATAGAGGCTAGAGAGATTAACATGGAGAGAAA 7 0.17500000000000002 No Hit TTGGGCAAGGGCTGAGGCGATGTGGTATGGTCTGCTTCTCCTTTGTTGAG 7 0.17500000000000002 No Hit CTCAAACTTCCTTGGCCTGGAAGGCCATAGTCCCTCTAAGAAGCTGGCCG 7 0.17500000000000002 No Hit GTTACAGCTTCAGGGCAATATAGGGTAGCCTATCCCTTCTCTTCCACATT 7 0.17500000000000002 No Hit CCTTTCGGCGCCAAGGCTTACGCTGCCTAACAATGACAGCAGGCATAACC 6 0.15 No Hit GGCGTTCCATAGGCTTGAGTATATCTTCATTGGCTGGTTGAAAGGAAATT 6 0.15 No Hit CCATCTTCAAGATCCTCAAGATCACCAGTTCTACGAGAACTATTTCGGTG 6 0.15 No Hit CCCTAACGTTGCCGTCAGCCGCCACGTCCCGGTTCAGGAATTTTAACCCG 6 0.15 No Hit GTTGAGAACAGAGATCGCAAGAAGAGGGAGACCACCGATAACCATATGCC 6 0.15 No Hit TGTTATTTAAAATGCTTGTCTTACAGTAAGCCCTACCTGTAGACATGGAA 6 0.15 No Hit GCAGCAACAAATCATCAGGAGGCCTATCACTGCCACTGCAACAACTGCAG 6 0.15 No Hit CTTCTCTAGCTTCATTAATAGAAACCCATCTTCTTTTTCGGATTTTGCTC 6 0.15 No Hit GTTATCCAAAAAATGTTTGTGGGGGTGGACATATTTTCTTTCAGTAAAAC 6 0.15 No Hit CTTCAACACAAATCACACAATATTGTCATTTACAGTCATCATATCCAACA 6 0.15 No Hit ATTGATTTAAAATATCAGAATCTCCCCGAGTCGGAGATCTAGAAAGAGAC 6 0.15 No Hit GGCCAAGTCTTTTCCCACAGTCTGCATATCAAACCCCAGTGAGGTTGCTT 6 0.15 No Hit GTCATGGAAAGTGAAGAGTTTATGGGGGCAGATACCAATCGTCGGTACCG 6 0.15 No Hit GGTAATTACAAGCTCTTAAGTTTTACGACTGGACAGCTAATTTTCTAGCA 6 0.15 No Hit GCCATCAGTATATAAGACCTTATGCTTCTTCTTAATCGAGTCGAAAGATA 6 0.15 No Hit CCCTTCACCACAAGATCAACACCATCATCGCTGTAATGCCAAGACGAATC 6 0.15 No Hit ACCGCCTCCGCCACCTTCACGGCGACCTCCGTAACCGCCACCGCCTCCAC 6 0.15 No Hit CACTAGACTTTGACCATGGATCCAATTGCCTGCTCGATCCAATCTAGCCC 6 0.15 No Hit GTGCTTCATGAGGATAGATGGATGAAATTTGGAACGAGAACACAAAAGTG 6 0.15 No Hit GACCTCTTCCAAGGGCTTGTAAGTGTAACACCAAGCACAAATCACAAAAT 6 0.15 No Hit CTCATATCCATTGGCTTCTTCCTATTCTCCTCAGCAAATACAACAGTCAA 6 0.15 No Hit CCCATACTCACATCATAACCCTCCACTAACTTCTCCACATCACCCTCAAT 6 0.15 No Hit TGTAAGTTCATCTTTAACTCCAGGGTGTGAGGATTTAACGAACTACGTGG 6 0.15 No Hit CTTCGCTCTTGTGCCATCAGCACCCACTAATGGAACCTCATCAACCTCTG 6 0.15 No Hit GCATCGTCGTGATGGGCAGAGGATGAAAAGCCTCGTCTAGTAGGAGCAGA 5 0.125 No Hit GTCGGGGTACATGCCACATTTGCAGCCACTGCCACACTTGCAGTCAGAGC 5 0.125 No Hit CATACTTCTGTGTCCAGCTACGGGCGGTGGCCTCATACTTTGCCCTGTCA 5 0.125 No Hit CAAGCTTTTTACAGGCAGACAACGGGTCTGCCTTCTCGACTCCTATGCAC 5 0.125 No Hit CCATTGTTATTAAGGCTTGCCCTAATCCTTCTATTTCCACTCCTTGTTTC 5 0.125 No Hit GCCAGGAGCGGGAGGTGATCGCTGCTCTGTGAGACCAGCCTCACGCTGTG 5 0.125 No Hit GCTTTGGATCATCTACTGCCGTCGCTGCTGCTGTGTCATTCACTGGAGCA 5 0.125 No Hit CAAGAAATTCGTTTTGGGTTTGTGGAGTAATGGAGTGATGAGGGCGTTGG 5 0.125 No Hit GGCAGCTTTGAAAACAGAATAATGTGACTCTCGTGAAGCATAAAGAACTC 5 0.125 No Hit TTGCTATTTGTCCAAAACTCATACTCAACCCGCTCATCAGGATGTGGAAG 5 0.125 No Hit GTCATTTCTTGTAGTAAATCAAGTATTTCTAGCTAAAGAGTTGAAGAGAT 5 0.125 No Hit ATAAATTTGAACCTCATCTTTTGGTTCCTTGCCTCTCACAGCAAAATCTT 5 0.125 No Hit GTTTGCTATTGTAATCGCACATTTTGAAATTTCACCTACAAGAATGGTCT 5 0.125 No Hit ATGGGAACTTGCTGCAGGGAAAACTCCCAAAAGCTGGTTGAATGGTTTGA 5 0.125 No Hit GTTCTCATCATCGAAGGAAACCTCCTCTTTAAAGACCCCGGCTTTCCCTC 5 0.125 No Hit CTTTAAATAATCGTTATCCATGCGAACCAATGCATCATGAATTTTTCCTG 5 0.125 No Hit CGGAAGCTTTAGTCTTAATGGGAGACAGAGGAGACAGAGGAGACACCAAA 5 0.125 No Hit GTTGGCGGGTCTCGGTTTCAGAGAAGGGAATAGCAAGCCATGGCATTTCG 5 0.125 No Hit AGGATGAGGCTGCACTGTGGTTAGACCATACAAAAACTCTCCAAAAAGGC 5 0.125 No Hit GCAGAAGTGGCAAGGAAGTCTATAATAATTCCACAAATGGTATAGTTTTT 5 0.125 No Hit GGCTTCCATCTCATTCTTACCAAATACCAACTGATATGTGACCATTGCAC 5 0.125 No Hit TTTATCTTTCATCCTCTCGGCCAAGGTCGCTCTTTTCCCCCCCTCTTCGA 5 0.125 No Hit GCGCCATTCCATTCCTTCAAGCATTCCAGCATGGGATCAATCAACTTCCC 5 0.125 No Hit ACTTGCTTATCTCTACTTGTTATGAGAACTTTACTGCCCGGACCAAATGA 5 0.125 No Hit CAGAGGGGCTCAGGAGTTGGACCTCTCTGGATGAGCTCAAAAGCATAGCC 5 0.125 No Hit GTGATGAGTGAATCATTGTTTTTATTATAAAACAAGCTTCGTATAACTTC 5 0.125 No Hit TGTCACGATCTTTAATGAGGACACAGAGACATTTCACACTCTTATCTAAC 5 0.125 No Hit GTCGATGCTCTCGTATGCAGTTGAAGAGTGAGGCGTGGATGTGTTAATGG 5 0.125 No Hit CTGGAATTACCAATACCTGATTTTCTGCAACTGCTTTCAGAAATTCAGTT 5 0.125 No Hit CATGAATGGCTGCATAAAGAATACCTGGATTATCAATATAAGGAATCTTC 5 0.125 No Hit GGTTCTTCTATGGCAACTTGCAGAGATTGAGAAAGTTCACCGAGTTCTAG 5 0.125 No Hit GCCCATCCATGATTCTTAAGACCTTCATAAAACCTGGTAACAATGATGCA 5 0.125 No Hit ATACAGCCTTAAACCTCTTACATCAAATGATACAAGATTCGGCTACTAGT 5 0.125 No Hit CCAACAACTCTTCAGGATAGCCAAGATTAGACACCAACTTTTCAATCGTG 5 0.125 No Hit GGTAATGGATATATGGATTGAATCTGCACATCAGCAGGGAACAGCTTGCA 5 0.125 No Hit ATTTTATGGCAACCTTCTTCAAACATCTCAAGAAATCCAGCAACCAAGCG 5 0.125 No Hit GCTGTTGTTTCATATCCACTAACTGGTGTATCATATAAAGATGAAGCCTT 5 0.125 No Hit GCCGAAAGGTGTTGTGGTTTGACCAACCCCTGCTCCGGCCACGCCATTCA 5 0.125 No Hit GTCCAATCTCCCCTCGACAAAATATTCCAGCCATTGGGAACCCGGGAAAA 5 0.125 No Hit AGGAGAACAAAACACAGGATATCCTATTGAAGTATGCTTCATTCTAGCCA 5 0.125 No Hit TCCAGAAACTAAAAGGCTCAATGGCCCAGAAACAAACAAGAAGGGAAATT 5 0.125 No Hit CCCGCATTATCTTGCTCTGAGAGATTCAATTCTTGCCTAGCTGCATAAGC 5 0.125 No Hit GGTTTTTCTCATCATCATCATCATCCTCTCCTCTTGATGGACACCAAGGT 5 0.125 No Hit GTTTGACATATCAGGGAACCTTATATTGCCAAAAGAAGTGGGTGGAACTG 5 0.125 No Hit CGACATGATTACGATGACCATGAATCTTCAACATACTTAACCTGATAGGA 5 0.125 No Hit CTGCACTTGCAATCCTAGGCCAAAGATCAGCACACTCCTTTCCAATTGGA 5 0.125 No Hit TATCAAATCAAGCTTCAGTATATCCCCTTCATCTGGATTTCCCGAACTAC 5 0.125 No Hit CCTTCATGCGTGCATTTTCTACCGCTAGTTTCTCCAACTCCTCCTGCTGT 5 0.125 No Hit GTTCAAGGGATTACTACAGAGCTGTTTAGATACAATGCACTGAACTTCTC 5 0.125 No Hit GCCGGAAGCGATAGAATCAGTGGAATGGAAACCGTATTGCTCTCTAGGAA 5 0.125 No Hit ACATGTAAACTAAGACAAAATTAAAATACCAACACTAGAACGACAACAGT 5 0.125 No Hit CTTTTTCAAAAAGTCACCATTGCAAAAGAGCAAAGCCTCCAATTATCTCA 5 0.125 No Hit CTCCTGGCTTCTTCGGATCTTCTTTCTTCTTTTCAGGCTCTTTTGCTGGA 5 0.125 No Hit GGATATAGGTCTGAGGCACGCCTCCACCATACATGGACCAAATAACCGGC 5 0.125 No Hit CTTCGAATCGATGATTTCACCGTACTGGCTAAAAGCCTCTTGGAGGACTT 5 0.125 No Hit GGGCATGTATTTCCCAATTGCACCAGCCTCGCAATCTCCATCTTGAGGGC 5 0.125 No Hit GTGATCCCATTTATCCTTCAGCCAAGCCCAAGCTGTTTCACGTCCTTCCT 5 0.125 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0 0.0 0.0 0.0 0.0 64-65 0.0 0.0 0.0 0.0 0.0 66-67 0.0 0.0 0.0 0.0 0.0 68-69 0.0 0.0 0.0 0.0 0.0 70-71 0.0 0.0 0.0 0.0 0.0 72-73 0.0125 0.0 0.0 0.0 0.0 74-75 0.05 0.0 0.0 0.0 0.0 76-77 0.075 0.0 0.0 0.0 0.0 78-79 0.125 0.0 0.0 0.0 0.0 80-81 0.125 0.0 0.0 0.0 0.0 82-83 0.15 0.0 0.0 0.0 0.0 84-85 0.15 0.0 0.0 0.0 0.0 86-87 0.175 0.0 0.0 0.0 0.0 88-89 0.30000000000000004 0.0 0.0 0.0 0.0 90-91 0.3375 0.0 0.0 0.0 0.0 92-93 0.3875 0.0 0.0 0.0 0.0 94-95 0.5625 0.0 0.0 0.0 0.0 96-97 0.7375 0.0 0.0 0.0 0.0 98-99 1.1749999999999998 0.0 0.0 0.0 0.0 100-101 1.3625 0.0 0.0 0.0 0.0 102-103 1.5 0.0 0.0 0.0 0.0 104-105 1.7375 0.0 0.0 0.0 0.0 106-107 1.975 0.0 0.0 0.0 0.0 108-109 2.375 0.0 0.0 0.0 0.0 110-111 2.7 0.0 0.0 0.0 0.0 112-113 3.0125 0.0 0.0 0.0 0.0 114-115 3.3125 0.0 0.0 0.0 0.0 116-117 3.6125 0.0 0.0 0.0 0.0 118-119 3.9000000000000004 0.0 0.0 0.0 0.0 120-121 4.35 0.0 0.0 0.0 0.0 122-123 4.775 0.0 0.0 0.0 0.0 124-125 5.387499999999999 0.0 0.0 0.0 0.0 126-127 6.300000000000001 0.0 0.0 0.0 0.0 128-129 7.2 0.0 0.0 0.0 0.0 130-131 7.7375 0.0 0.0 0.0 0.0 132-133 8.25 0.0 0.0 0.0 0.0 134-135 8.9875 0.0 0.0 0.0 0.0 136-137 9.524999999999999 0.0 0.0 0.0 0.0 138-139 10.375 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTTTGCT 10 0.006830828 145.0 1 TACATAT 10 0.006830828 145.0 9 CCATTTC 10 0.006830828 145.0 2 AAAAAAA 35 0.0035366106 20.714287 60-64 >>END_MODULE SRR26075366 read2 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR26075366_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.18275 37.0 37.0 37.0 37.0 37.0 2 36.2045 37.0 37.0 37.0 37.0 37.0 3 36.307 37.0 37.0 37.0 37.0 37.0 4 36.16 37.0 37.0 37.0 37.0 37.0 5 36.2355 37.0 37.0 37.0 37.0 37.0 6 36.1535 37.0 37.0 37.0 37.0 37.0 7 36.2505 37.0 37.0 37.0 37.0 37.0 8 36.1355 37.0 37.0 37.0 37.0 37.0 9 36.084 37.0 37.0 37.0 37.0 37.0 10-14 36.0559 37.0 37.0 37.0 37.0 37.0 15-19 36.018899999999995 37.0 37.0 37.0 37.0 37.0 20-24 35.94689999999999 37.0 37.0 37.0 37.0 37.0 25-29 35.8661 37.0 37.0 37.0 37.0 37.0 30-34 35.8279 37.0 37.0 37.0 37.0 37.0 35-39 35.7042 37.0 37.0 37.0 37.0 37.0 40-44 35.7293 37.0 37.0 37.0 37.0 37.0 45-49 35.71640000000001 37.0 37.0 37.0 37.0 37.0 50-54 35.5719 37.0 37.0 37.0 37.0 37.0 55-59 35.5674 37.0 37.0 37.0 37.0 37.0 60-64 35.5686 37.0 37.0 37.0 37.0 37.0 65-69 35.5544 37.0 37.0 37.0 37.0 37.0 70-74 35.4613 37.0 37.0 37.0 37.0 37.0 75-79 35.436899999999994 37.0 37.0 37.0 37.0 37.0 80-84 35.440599999999996 37.0 37.0 37.0 37.0 37.0 85-89 35.4009 37.0 37.0 37.0 37.0 37.0 90-94 35.34935 37.0 37.0 37.0 37.0 37.0 95-99 35.3604 37.0 37.0 37.0 37.0 37.0 100-104 35.2272 37.0 37.0 37.0 34.6 37.0 105-109 35.2806 37.0 37.0 37.0 37.0 37.0 110-114 35.2034 37.0 37.0 37.0 34.6 37.0 115-119 35.161500000000004 37.0 37.0 37.0 34.6 37.0 120-124 35.043600000000005 37.0 37.0 37.0 29.8 37.0 125-129 35.02355 37.0 37.0 37.0 29.8 37.0 130-134 35.069900000000004 37.0 37.0 37.0 29.8 37.0 135-139 34.919799999999995 37.0 37.0 37.0 27.4 37.0 140-144 34.873200000000004 37.0 37.0 37.0 25.0 37.0 145-149 34.7313 37.0 37.0 37.0 25.0 37.0 150-151 34.549625000000006 37.0 37.0 37.0 25.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.0 1101 2 0.0 1101 3 0.0 1101 4 0.0 1101 5 0.0 1101 6 0.0 1101 7 0.0 1101 8 0.0 1101 9 0.0 1101 10-14 0.0 1101 15-19 0.0 1101 20-24 0.0 1101 25-29 0.0 1101 30-34 0.0 1101 35-39 0.0 1101 40-44 0.0 1101 45-49 0.0 1101 50-54 0.0 1101 55-59 0.0 1101 60-64 0.0 1101 65-69 0.0 1101 70-74 0.0 1101 75-79 0.0 1101 80-84 0.0 1101 85-89 0.0 1101 90-94 0.0 1101 95-99 0.0 1101 100-104 0.0 1101 105-109 0.0 1101 110-114 0.0 1101 115-119 0.0 1101 120-124 0.0 1101 125-129 0.0 1101 130-134 0.0 1101 135-139 0.0 1101 140-144 0.0 1101 145-149 0.0 1101 150-151 0.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 12 3.0 13 5.0 14 14.0 15 19.0 16 15.0 17 10.0 18 4.0 19 8.0 20 5.0 21 3.0 22 9.0 23 17.0 24 10.0 25 16.0 26 13.0 27 13.0 28 19.0 29 15.0 30 30.0 31 18.0 32 36.0 33 85.0 34 162.0 35 620.0 36 2640.0 37 211.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 46.41160290072518 22.48062015503876 9.102275568892223 22.005501375343837 2 27.900000000000002 23.875 26.674999999999997 21.55 3 24.5 26.924999999999997 31.175000000000004 17.4 4 26.05 36.05 20.724999999999998 17.175 5 26.05 37.225 19.1 17.625 6 23.25 35.25 23.400000000000002 18.099999999999998 7 23.075000000000003 22.05 35.625 19.25 8 24.5 24.75 26.450000000000003 24.3 9 24.425 25.5 27.825 22.25 10-14 25.745 29.24 25.174999999999997 19.84 15-19 24.725 28.09 26.595000000000002 20.59 20-24 24.975 28.555000000000003 25.790000000000003 20.68 25-29 24.695 28.68 26.465 20.16 30-34 24.224999999999998 28.78 26.915 20.080000000000002 35-39 23.875 28.689999999999998 26.640000000000004 20.794999999999998 40-44 24.08 29.21 26.295 20.415 45-49 24.84 27.150000000000002 27.775 20.235 50-54 23.825 28.444999999999997 27.315 20.415 55-59 24.345 28.835 26.834999999999997 19.985 60-64 25.035 29.294999999999998 26.145000000000003 19.525000000000002 65-69 24.14 29.445 26.640000000000004 19.775000000000002 70-74 24.099999999999998 28.33 26.965 20.605 75-79 22.99 28.615000000000002 27.76 20.635 80-84 24.22 29.049999999999997 26.85 19.88 85-89 25.474999999999998 28.38 26.38 19.765 90-94 25.09125456272814 28.821441072053606 26.986349317465873 19.10095504775239 95-99 24.545 29.45 25.955000000000002 20.05 100-104 25.724999999999998 28.895 26.51 18.87 105-109 25.014999999999997 29.115000000000002 25.96 19.91 110-114 25.169999999999998 28.7 26.075 20.055 115-119 24.884999999999998 29.365000000000002 26.145000000000003 19.605 120-124 25.367536753675367 28.91289128912891 26.852685268526855 18.866886688668867 125-129 25.29126456322816 29.556477823891193 26.126306315315766 19.025951297564877 130-134 26.165 28.95 25.555 19.33 135-139 25.8501700340068 29.200840168033608 25.775155031006204 19.17383476695339 140-144 25.99779933980194 28.37851355406622 26.72301690507152 18.90067020106032 145-149 26.715686274509803 29.001600640256104 25.07002801120448 19.21268507402961 150-151 28.785794673002375 28.03551331749406 25.534575465799676 17.644116543703888 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.5 4 1.0 5 1.5 6 1.5 7 2.0 8 3.5 9 2.0 10 0.5 11 1.0 12 2.0 13 2.5 14 1.0 15 0.5 16 1.5 17 2.0 18 1.0 19 0.0 20 0.5 21 1.0 22 1.0 23 1.5 24 3.5 25 2.5 26 0.0 27 3.0 28 4.0 29 3.0 30 5.0 31 6.0 32 15.5 33 27.5 34 37.0 35 53.0 36 63.0 37 86.0 38 117.0 39 168.0 40 220.5 41 243.0 42 282.5 43 288.0 44 271.0 45 280.0 46 289.5 47 251.5 48 203.5 49 190.0 50 167.5 51 132.5 52 114.0 53 97.0 54 72.0 55 51.5 56 35.0 57 37.0 58 25.0 59 13.5 60 17.5 61 11.0 62 4.5 63 6.0 64 6.0 65 3.5 66 6.0 67 5.5 68 1.0 69 1.5 70 1.0 71 0.5 72 1.5 73 2.5 74 2.5 75 1.0 76 2.0 77 3.0 78 2.5 79 1.5 80 0.0 81 0.5 82 0.5 83 1.5 84 2.0 85 2.0 86 2.5 87 2.0 88 1.0 89 0.0 90 0.5 91 0.5 92 0.5 93 0.5 94 0.0 95 0.5 96 0.5 97 0.0 98 0.5 99 0.5 100 8.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.025 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.005 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.01 125-129 0.005 130-134 0.0 135-139 0.02 140-144 0.03 145-149 0.04 150-151 0.0375 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 57.99999999999999 #Duplication Level Percentage of deduplicated Percentage of total 1 62.88793103448276 36.475 2 19.82758620689655 23.0 3 8.793103448275861 15.299999999999999 4 4.051724137931034 9.4 5 2.5 7.249999999999999 6 0.9482758620689655 3.3000000000000003 7 0.47413793103448276 1.925 8 0.21551724137931033 1.0 9 0.08620689655172414 0.44999999999999996 >10 0.21551724137931033 1.9 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG 23 0.575 No Hit ATGCAAAAGGCTGCAGCTGCCGATGCACCAGCACCAAGCCCAACATCTGA 15 0.375 No Hit TTTATGGAAAGTCCTGGCCGAGCAGTTCGGGATAGAAAAGTACGGGTTCG 14 0.35000000000000003 No Hit CATAGAACTTGTTTTTATCTTTTGAAGCATATTTGTCTACTCTCTTTTCG 13 0.325 No Hit GGAAAGAGGGTAGAAATATCCAAGTTTGAAACTGAATATCGAGAGGTATT 11 0.27499999999999997 No Hit CTTCTTTTCAAGATTAGGGTTTTTGTTTTCAAGAAAACGGCATGGGTGAG 9 0.22499999999999998 No Hit GTGTGTCGCCGCAGCAGTAGAATAAGAAGGATGTCTCACAGGAAGTTCGA 9 0.22499999999999998 No Hit CTTGAACACTTTGCTGATTGATAATGACCCCTACAAGTCTCTTCTAAATC 8 0.2 No Hit ATTTGAAGCAATTTAACAGCGAAAAAACAAGTGGGCTTTATAGTATTGCC 8 0.2 No Hit CAATGTCATACCTCAAAGGGATAGCTCAAATGTATGAAAATGGAGTCCTC 8 0.2 No Hit AGAGACCCAATGAAGTACCCTTACAGCTGTGTCCCTTGCCCTGAATTTCG 8 0.2 No Hit CCATCTTGCAAGAAAAAAACAATGGCAGACACACTCACAAATCTCACACC 8 0.2 No Hit TTTTCAGTATCTCCGCCACCGAGTTCGCTTCCTTTACCGTTGTTCTCAAA 7 0.17500000000000002 No Hit GCTTGTTGGAGGTTTTGTTTTCTCTTGTTGTGGCCGTGGTGAATCATTTT 7 0.17500000000000002 No Hit CCGGCTGGTTCCACCATTTCCTGCCCCTCTTCACAGCTTCCTCAGCCCTT 7 0.17500000000000002 No Hit ATGAAATTCAAGAGAAGCTTCGGCAGGGTCACAATATTGTCTTGATATCA 7 0.17500000000000002 No Hit GCAGATTCATTCGCCAACTAACCCTTTAATTTATCCTATTTTTCCTAAAG 7 0.17500000000000002 No Hit GGGAGGTGAAGTGTGCCCAAATTCTTCTCGAGGCCGGGGCAACTGTTGAT 7 0.17500000000000002 No Hit GCGAGCAAAAAAAAAAGAGAGATACTTTGGGAGAATTCAATAGGGACTGG 7 0.17500000000000002 No Hit CCAAAACTGCTGCTGCTCCCATTGAGCGTGTTAAGCTTTTGATTCAAAAC 7 0.17500000000000002 No Hit ACAGTTCAGGCAATTTGCCCCAAGGCAACCGTTTCCTTTCAGCAGCAGAT 7 0.17500000000000002 No Hit GTCTGATTCCGAGGAGGTTTCTGAGCTGGAGTGAGTCAGAGTACATGCAG 7 0.17500000000000002 No Hit ACCACCAACAACAACATAGTTCAAAACGACAAGACGTTGTCAAAGAAAAG 7 0.17500000000000002 No Hit GGAGATCATAAAGCAGCAGTGTGTCACAAGATGAGATATCCTTATGCAGT 6 0.15 No Hit GTTACCTCGATACCTCCAACCAGTGGCTTGTGAGGCCAGTTCTAGAAACT 6 0.15 No Hit GATAGTTGACGGATCTATCTACCTTTACCAAAAGAAAGATGGATCTCTCG 6 0.15 No Hit TTTGTCGACAGGGAGAATGATGTACTTCTCCTTGGAGATGATCCCTGGGA 6 0.15 No Hit GTGCTTTTCATATTTGATCAAATCTTGTTCCTTGCATCTTTTATCATCGT 6 0.15 No Hit GACAATAGCAGGAAACAGAGGAAGAGGCATCAAAATGCTGTGGATGATCT 6 0.15 No Hit AGAACTTTGAAGGCCGAAGAGGGGAAAGGTTCCATGTGAACGGCACTTGC 6 0.15 No Hit GCACTGTTATCAAGCCCAGAAGCCGTGTTTGGAATTAGATTTGTGATTGA 6 0.15 No Hit TAGGAATCATCGATCAAGAAGAGGAAATTAAGAAGGAAGTTCGAATCAAG 6 0.15 No Hit GGCCCCTTCTCTTTATATGGTTGAGGTTCGCAAGTCTGATGGTGATACCC 6 0.15 No Hit GTTACACAAAAATGGCGAACCGTCCCTTTTTCGACTATCTAAACAAAAGT 6 0.15 No Hit AACATGCATAAGCCTTAGTATGGGAGAAGCATTCTCAGGAGCTTTTGCCA 6 0.15 No Hit AATCATCGATTCGAAGATTATAAACGATCGTGAAACTGGAAGATCTCGCG 6 0.15 No Hit CCAACTTCTCTTTCCACAATTTCCCACCAGTCTTCTCATCTTATGAAGAA 6 0.15 No Hit GAGGAAGCGTGCTGCTGGTGATGAAAAAGCACCTGATATTAAAGAATTTG 6 0.15 No Hit CCGTGGTTTTGGCTTTGTCCAATTTGCAGATCCTCATGATGCTGCTGAGG 6 0.15 No Hit GGGGTAAACTAGGCCCGTGGCAGTATAAGAGCAAAAGGAGTAGCATAATG 6 0.15 No Hit AGGCCTTTTACTTCTTGAGGTACCTACTCTGACATTTGATGAAAGCAATT 6 0.15 No Hit TCCAACTTTCCACTGGCAAAGACTGCTTGATCTTTCAACTCCTGCACTGT 6 0.15 No Hit GGAATGTCAGAAGTCTTCATGGCTGTTGGACAGTTAGAATTTCTATATGA 6 0.15 No Hit GACTGGCCAGCCTTACATTTTGCAGACAAATGTTTTCACAGGAGGCAAGG 6 0.15 No Hit GTCCAGAAAAGAGGCAGAATTTGCTCTTGCAGCATTGATGGAAATACCTT 6 0.15 No Hit ACCAGCTTGAGCAAATTCAGTTTCTAAGGAAAAGCTTTCCAGGCCCCTTT 5 0.125 No Hit ATTAGGAAGTATAATCTCCTTGGTAATCTTGGAGAATCTGAGGATACTGA 5 0.125 No Hit GATAGAGAATATATTGATGATCCTAATGATGTCAAACCTGAGGGATATGA 5 0.125 No Hit GGATATTCAAGAAAAGAGCTTGGTGTTAGCTACGTAAAGGAAAAGCGACT 5 0.125 No Hit GTAGAGCAATGACTGTGATTTCGATATGTTCATGATTACTCTTACAGGCA 5 0.125 No Hit GGCGCAACTCGGTTCGTTTAGGTTGTGCTAAAGCAAGGTGCAGCAATGGC 5 0.125 No Hit GAAACCCAAAAGCGACAGAGACCCATTACCAATTTCAACAGGAGCGTCGT 5 0.125 No Hit AGAAAACAATGGCACTACATGGAAAGATTGAGACAACATTAGAACTCAAG 5 0.125 No Hit GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGT 5 0.125 No Hit GTAAGGTCGGGTTCAGGTTGTCTCTTATCTGGCCCGCCCGTTCCAAGCCA 5 0.125 No Hit TTATATATTGCCACAGATGGTCGGTCAAGATGGCAACCTCAACTCCCTCC 5 0.125 No Hit TGTCACTGGGTCTGCCAGTGGCAGCAACAGTGAACTGTGCTGATAACACT 5 0.125 No Hit GTGAAAGTTTATTTGGTTACTGGCCTTGTCTATCTCGGTATGCTAGGAAT 5 0.125 No Hit CAGGATAAGTTTATATATAGTTGCTTCAAACATATCTGAAACGCTGTAAC 5 0.125 No Hit CCCGATGATGGGTGCCGAGGACTTCTCGTTCTACACACAAGTGGTCCCTG 5 0.125 No Hit GGATTTGTGGCATAAGATGATCTTCCCTGTTGGGAGAGTTTGGCTTTCTG 5 0.125 No Hit GGGAAGAGTATAACGTGGGTAGTGGGGATGCCAATGCCTGCTTTCAAGGG 5 0.125 No Hit GACAGCATTTGCAGCTGTGACTTCAGGAAGCTTGAATCTTCCAAGTGGCA 5 0.125 No Hit AAAAAGAAAGCAGAGAGAGTGAATGGAGAAGAAGAGCAGCAGAGACAGAG 5 0.125 No Hit GATAATTGGGATGACGAATACTCCATCATCGGGGACAAGGGTGAGATTGG 5 0.125 No Hit GAAAGATGAAGCTGAACTTGGAAAACGGATCTTCTGCAATAGGTCATTGA 5 0.125 No Hit TTCAAAGAAGTGGAAGGCAACGTTTGAGAGTGCTGAGCTGTTTAAAGTTA 5 0.125 No Hit GAAACAACACAAGTAAGAACCGAAAAGAATTTCACACGACACCAGAGTAG 5 0.125 No Hit GTCAAATCTTATACCTGGATATGGGGATCCTTTTGGGCCCCAAATACTCA 5 0.125 No Hit CCTAGCATCAATACCTAAATCAGGAACTACCTGGCTCAAAGCCCTATCTT 5 0.125 No Hit GCTTTATCGAACTCTTTGCGACACACAGGAGAAATGATCAAGCTGTTTAA 5 0.125 No Hit GCTAGACAGCCATGGATGAGGCAGTAGTTCTCTACCCATCACCAACAATC 5 0.125 No Hit GCAAGAAAAAGAAAGAGAAGAAGAAATGGCGATTTCTATGATGCGATCTG 5 0.125 No Hit GTTGGATAAAAAAATCTCAAAAAAACATTACAGTCACAATAGTACGCATA 5 0.125 No Hit GTGATGCTGTCATGTATCCAGCACCGGAATTGGTAGAGAAGGAAGCAGAG 5 0.125 No Hit CCCTAATCTTGTAGTTTTTGTTCTTTTTGTAAATTATGGGGAAACAGATG 5 0.125 No Hit TTTCTAAAAAGCCTCATGCTAGATTTTTTTTTATTTTTTATTTTTTATTA 5 0.125 No Hit GGTGTCTTTTCGTTTCTTTCAAGGCCAAATACTCCTCTCTCAGAAAATGG 5 0.125 No Hit ACTTCAGGTGTGGTACTGGCTGTATCCTTAGAAGTGAGCTCGAGTTTAAA 5 0.125 No Hit AGACAATCCACGCGACAGAAAAAAAAGAAATGACAAAGAATATCGAGCCC 5 0.125 No Hit GCACTTATTAGCAAGTCCTTGGGGGATAGTATCAAGAAAGCTATATCTAA 5 0.125 No Hit GTTCTGCTCAAATATTGCAATTGACCATACCCTTGTTTGTTGCACTTAGG 5 0.125 No Hit GAAAACGCGCATTCTGGGTTCAATAGCATCTTGTCCAGATCCAAACATAA 5 0.125 No Hit CTGCACCATGCACTCTATCCCTGCATCATCCCTTGAGCCAGGTGGGTCTG 5 0.125 No Hit GGCAATGGTTAGATCAAACATCATAAATGGAGAATTTGCTTTCAGTCAAA 5 0.125 No Hit GGTGAACTGGGCAGCAGGATTTCAGAAGATGAATATAGAGAGATTTTTCT 5 0.125 No Hit GAGATATACAGAAGGGCTGTGTTGGGTCATGCATTATTATTATGAAGGTG 5 0.125 No Hit AGATGAACAGGTGGCAAAAGACATACAACTCGTAGATTCTATTGCAGAGA 5 0.125 No Hit CCCATTCATTGAGAGTAACTACGGTGTCCATTCAAGACAGTTTGAAGTTG 5 0.125 No Hit GCTATGCGGAGAGTCTCTGGTCTTCCAGGGGTGGATTCAATATCCATGGA 5 0.125 No Hit GTTCTAGTTGATCACGCCCTGATGAATGGTGAGAAGGAACATAATTCAAG 5 0.125 No Hit ACATGGGCTCCTTGAGTTTTCGAGATTCTTTTGTGAGGGAGAGACTTCGT 5 0.125 No Hit GTTGCAAACTCCTGGCAAACCACGTTTTCTAAATCCTGCATCCAAGGACA 5 0.125 No Hit GATGCAGTAGAACTTACAAATTGTCTGAGAAAGAAAGTAGGCTACGCAGA 5 0.125 No Hit CTTCTCAACATCAATCTTATTCTACCCATCGCTCTTGAGCTCCTTGTTGA 5 0.125 No Hit CCTGCTTCCATGTTGGAAACAGATGCTGCTAAGGAGAAACAGAGTATTGA 5 0.125 No Hit GGCTGAGGTTAGAGCACAGAATGCATTGGCTGAGGTTCAAGAATTCACAT 5 0.125 No Hit GGATGATGCCAAGGAAGCCAAACATCTGCCACTTCCTTCAGCATGTAGCC 5 0.125 No Hit GGAACTTGGAGAATTCAGGGCTGCTTGGCAGTGTCTAGGGGAATTGGAGA 5 0.125 No Hit CAGCAATGGAAGCATATGTCTGGATATTCTTAAAGAGCAGTGGAGCCCTG 5 0.125 No Hit GTGAAAACCTCTCCTTATCAGGCACTCACCAAACTTTTCTTCCTAACCAA 5 0.125 No Hit GTGGGATTCTATTTCTCTGGTTCGTGGTGCGGCCCATGCCGTAATTTCAC 5 0.125 No Hit CTCAAATTCTGCACCTCTATCTTCTTGCAACTCTACTTCTTGCTCTTTCT 5 0.125 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0 0.0 0.0 0.0 0.0 64-65 0.0 0.0 0.0 0.0 0.0 66-67 0.0 0.0 0.0 0.0 0.0 68-69 0.0 0.0 0.0 0.0 0.0 70-71 0.0 0.0 0.0 0.0 0.0 72-73 0.0125 0.0 0.0 0.0 0.0 74-75 0.05 0.0 0.0 0.0 0.0 76-77 0.075 0.0 0.0 0.0 0.0 78-79 0.125 0.0 0.0 0.0 0.0 80-81 0.1375 0.0 0.0 0.0 0.0 82-83 0.175 0.0 0.0 0.0 0.0 84-85 0.175 0.0 0.0 0.0 0.0 86-87 0.2 0.0 0.0 0.0 0.0 88-89 0.32499999999999996 0.0 0.0 0.0 0.0 90-91 0.3625 0.0 0.0 0.0 0.0 92-93 0.4125 0.0 0.0 0.0 0.0 94-95 0.5875 0.0 0.0 0.0 0.0 96-97 0.7625 0.0 0.0 0.0 0.0 98-99 1.275 0.0 0.0 0.0 0.0 100-101 1.4625 0.0 0.0 0.0 0.0 102-103 1.6 0.0 0.0 0.0 0.0 104-105 1.8375 0.0 0.0 0.0 0.0 106-107 2.0875 0.0 0.0 0.0 0.0 108-109 2.475 0.0 0.0 0.0 0.0 110-111 2.8 0.0 0.0 0.0 0.0 112-113 3.1125 0.0 0.0 0.0 0.0 114-115 3.4125 0.0 0.0 0.0 0.0 116-117 3.7375 0.0 0.0 0.0 0.0 118-119 4.05 0.0 0.0 0.0 0.0 120-121 4.5 0.0 0.0 0.0 0.0 122-123 4.9375 0.0 0.0 0.0 0.0 124-125 5.5625 0.0 0.0 0.0 0.0 126-127 6.4625 0.0 0.0 0.0 0.0 128-129 7.4125 0.0 0.0 0.0 0.0 130-131 7.9624999999999995 0.0 0.0 0.0 0.0 132-133 8.5 0.0 0.0 0.0 0.0 134-135 9.3125 0.0 0.0 0.0 0.0 136-137 9.8875 0.0 0.0 0.0 0.0 138-139 10.725 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GCAAGAT 10 0.006830828 145.0 7 TTTTTGC 10 0.006830828 145.0 4 GGTTTTT 10 0.006830828 145.0 2 TTTTTTT 35 0.0035366106 20.714287 95-99 >>END_MODULE Read 967594 spots for SRR26075366.sra Written 967594 spots for SRR26075366.sra Read 967594 spots for SRR26075366.sra Written 967594 spots for SRR26075366.sra Read 967594 spots for SRR26075366.sra Written 967594 spots for SRR26075366.sra Read 967594 spots for SRR26075366.sra Written 967594 spots for SRR26075366.sra Read 967594 spots for SRR26075366.sra Written 967594 spots for SRR26075366.sra Read 967594 spots for SRR26075366.sra Written 967594 spots for SRR26075366.sra Read 967594 spots for SRR26075366.sra Written 967594 spots for SRR26075366.sra Read 967594 spots for SRR26075366.sra Written 967594 spots for SRR26075366.sra Read 967594 spots for SRR26075366.sra Written 967594 spots for SRR26075366.sra Read 967594 spots for SRR26075366.sra Written 967594 spots for SRR26075366.sra Read 967594 spots for SRR26075366.sra Written 967594 spots for SRR26075366.sra Read 967594 spots for SRR26075366.sra Written 967594 spots for SRR26075366.sra Read 967594 spots for SRR26075366.sra Written 967594 spots for SRR26075366.sra Read 967594 spots for SRR26075366.sra Written 967594 spots for SRR26075366.sra Read 967607 spots for SRR26075366.sra Written 967607 spots for SRR26075366.sra Read 967594 spots for SRR26075366.sra Written 967594 spots for SRR26075366.sra Read 967594 spots for SRR26075366.sra Written 967594 spots for SRR26075366.sra Read 967594 spots for SRR26075366.sra Written 967594 spots for SRR26075366.sra Read 967594 spots for SRR26075366.sra Written 967594 spots for SRR26075366.sra Read 967594 spots for SRR26075366.sra Written 967594 spots for SRR26075366.sra SRR ids: ['SRR26075366.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_6wcgzdr7 SRR26075366.sra spots: 19351893 blocks: [[1, 967594], [967595, 1935188], [1935189, 2902782], [2902783, 3870376], [3870377, 4837970], [4837971, 5805564], [5805565, 6773158], [6773159, 7740752], [7740753, 8708346], [8708347, 9675940], [9675941, 10643534], [10643535, 11611128], [11611129, 12578722], [12578723, 13546316], [13546317, 14513910], [14513911, 15481504], [15481505, 16449098], [16449099, 17416692], [17416693, 18384286], [18384287, 19351893]] SRR26075366 file size 7141518 SRR26075366 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o SRR26075366 SRR26075366_1.fastq SRR26075366_2.fastq Input file: SRR26075366_1.fastq Paired file: SRR26075366_2.fastq trimmed: SRR26075366-trimmed-pair1.fastq, SRR26075366-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Tue Feb 11 22:52:17 2025 >> started Tue Feb 11 22:52:42 2025 >> done (25.131s) 19351893 read pairs processed; of these: 73 ( 0.00%) short read pairs filtered out after trimming by size control 26086 ( 0.13%) empty read pairs filtered out after trimming by size control 19325734 (99.86%) read pairs available; of these: 2888792 (14.95%) trimmed read pairs available after processing 16436942 (85.05%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 18 11 0.00% 19 10 0.00% 20 21 0.00% 21 14 0.00% 22 11 0.00% 23 9 0.00% 24 20 0.00% 25 22 0.00% 26 21 0.00% 27 27 0.00% 28 22 0.00% 29 18 0.00% 30 19 0.00% 31 25 0.00% 32 29 0.00% 33 24 0.00% 34 34 0.00% 35 38 0.00% 36 24 0.00% 37 37 0.00% 38 22 0.00% 39 26 0.00% 40 53 0.00% 41 31 0.00% 42 43 0.00% 43 52 0.00% 44 53 0.00% 45 48 0.00% 46 82 0.00% 47 61 0.00% 48 88 0.00% 49 69 0.00% 50 114 0.00% 51 138 0.00% 52 145 0.00% 53 125 0.00% 54 154 0.00% 55 142 0.00% 56 179 0.00% 57 240 0.00% 58 266 0.00% 59 304 0.00% 60 384 0.00% 61 365 0.00% 62 451 0.00% 63 471 0.00% 64 473 0.00% 65 596 0.00% 66 712 0.00% 67 720 0.00% 68 882 0.00% 69 874 0.00% 70 1149 0.01% 71 1351 0.01% 72 1419 0.01% 73 1680 0.01% 74 1952 0.01% 75 2222 0.01% 76 2430 0.01% 77 2492 0.01% 78 2899 0.02% 79 3408 0.02% 80 3685 0.02% 81 4340 0.02% 82 5043 0.03% 83 5650 0.03% 84 6201 0.03% 85 6669 0.03% 86 7335 0.04% 87 8058 0.04% 88 8712 0.05% 89 9470 0.05% 90 9906 0.05% 91 11204 0.06% 92 11806 0.06% 93 13564 0.07% 94 14540 0.08% 95 15445 0.08% 96 16811 0.09% 97 17400 0.09% 98 18285 0.09% 99 19007 0.10% 100 19825 0.10% 101 21391 0.11% 102 23191 0.12% 103 24535 0.13% 104 26585 0.14% 105 27448 0.14% 106 28544 0.15% 107 29825 0.15% 108 30396 0.16% 109 30724 0.16% 110 32088 0.17% 111 33564 0.17% 112 35000 0.18% 113 36663 0.19% 114 38139 0.20% 115 40079 0.21% 116 41261 0.21% 117 42558 0.22% 118 43777 0.23% 119 44645 0.23% 120 45073 0.23% 121 45818 0.24% 122 47431 0.25% 123 49808 0.26% 124 52189 0.27% 125 52857 0.27% 126 54749 0.28% 127 55138 0.29% 128 56480 0.29% 129 57054 0.30% 130 58199 0.30% 131 58688 0.30% 132 59238 0.31% 133 62720 0.32% 134 63673 0.33% 135 65709 0.34% 136 65911 0.34% 137 67233 0.35% 138 69260 0.36% 139 69777 0.36% 140 70261 0.36% 141 71281 0.37% 142 71555 0.37% 143 73443 0.38% 144 75708 0.39% 145 76492 0.40% 146 78297 0.41% 147 78703 0.41% 148 80092 0.41% 149 80289 0.42% 150 82496 0.43% 151 16436942 85.05% 19325734 reads passed initial QC criterion=sequence-density sequence-density=0.35 sequence-density-rank=1 fanout-score=3.36 fanout-score-rank=27 prefix-density=0.54 prefix-fanout=2.2 sequence=CACTTGCAGCCATTCTCAGCACCA criterion=fanout-score sequence-density=0.06 sequence-density-rank=31 fanout-score=53.79 fanout-score-rank=1 prefix-density=0.25 prefix-fanout=14.0 sequence=TTCTTGATAAATTTCTTAATCTGTGTAAGAAACTGCTTCTTGTCAAATGGAGGTTGCTCCTGGAGCCTAAATGTGTCAACGATGTCAACAACCTTGGCAGCTTGGTCATCAACACCCTCATCCTCATCACCTCCTTCAGCTGAAGGATTTGCACCAATGTCTACATCAACGGCTCCTTGAACAACCCACTTTCCTTCAACTTCCCACAGTATCCCATTCTCAATCTCCTTGTATGGGAACGAATCCGAGAGAAGCTCATCACCAGAGAGAAGATCTTGATA criterion=sequence-density sequence-density=0.41 sequence-density-rank=1 fanout-score=2.87 fanout-score-rank=27 prefix-density=0.41 prefix-fanout=2.8 sequence=ATGTACCCTGACTTAGGTTTCTCAGA criterion=fanout-score sequence-density=0.01 sequence-density-rank=31 fanout-score=266.12 fanout-score-rank=1 prefix-density=0.17 prefix-fanout=13.2 sequence=TTTCTTTGAGAGTGCATAGATTTGTGTTGATATAGAAAACAATGGCACTACATGGAAAGATTGAGACAACATTAGAACTCAAGTCCTCCGCAGAGAAGTTCTACAAAGTGTGGAGGAGCCAGTCCTTCCATGTTCCCAAACATGCTTCCAAGCATATCCAAGGAGTTGATATACATGCAGGTGACTGGGAGACTGCGGGCTCTATCAGGATTTGGCAGTACACAATCGGAGGGAAAGCCGGGGTCTTTAAAGAGGAGGTTTCCTTCGATGATGAGAACAAGATCATAACTCTTAATGGTTTGGAAGGAGAT SRR26075366 testing PE reads STAR mapping to Ensembl genome Started job on | Feb 11 22:53:26 Started mapping on | Feb 11 22:53:26 Finished on | Feb 11 22:56:21 Mapping speed, Million of reads per hour | 397.56 Number of input reads | 19325734 Average input read length | 294 UNIQUE READS: Uniquely mapped reads number | 17044934 Uniquely mapped reads % | 88.20% Average mapped length | 292.71 Number of splices: Total | 14987594 Number of splices: Annotated (sjdb) | 14636902 Number of splices: GT/AG | 14734324 Number of splices: GC/AG | 187276 Number of splices: AT/AC | 15272 Number of splices: Non-canonical | 50722 Mismatch rate per base, % | 0.41% Deletion rate per base | 0.03% Deletion average length | 3.12 Insertion rate per base | 0.02% Insertion average length | 2.55 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 630857 % of reads mapped to multiple loci | 3.26% Number of reads mapped to too many loci | 144697 % of reads mapped to too many loci | 0.75% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 7.12% % of reads unmapped: other | 0.67% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 1649943 1649943 1649943 N_multimapping 630857 630857 630857 N_noFeature 437131 16825560 554952 N_ambiguous 227882 1852 125046 UnstrandedReadsAssigned:16379921 PositiveStrandReadsAssigned:217522 NegativeStrandReadsAssigned:16364936 Dataset is classified negative stranded MeadianReadLen=151 20thPercentileLength=151 echo kmer=147 SRR26075366 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,400 [index] number of k-mers: 62,057,036 [index] number of equivalence classes: 130,681 [quant] running in paired-end mode [quant] will process pair 1: SRR26075366-trimmed-pair1.fastq SRR26075366-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 19,325,734 reads, 16,838,861 reads pseudoaligned [quant] estimated average fragment length: 214.06 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,179 rounds 52401 SRR26075366.ke.tsv 34699 SRR26075366.se.tsv 87100 total ==> SRR26075366.ke.tsv <== target_id length eff_length est_counts tpm Potri.005G200100.1.v4.1 2018 1804.94 3427 93.9665 Potri.005G024800.1.v4.1 1035 821.94 1398 84.176 Potri.004G059700.1.v4.1 961 747.947 54 3.5731 Potri.007G009000.2.v4.1 1416 1202.94 0 0 Potri.003G141000.2.v4.1 2943 2729.94 568 10.2971 Potri.016G087400.1.v4.1 270 89.3968 1294 716.364 Potri.015G069301.1.v4.1 564 353.207 0 0 Potri.010G195200.1.v4.1 1773 1559.94 117 3.71193 Potri.012G127500.1.v4.1 977 763.94 8662 561.152 ==> SRR26075366.se.tsv <== Potri.001G166300.v4.1 0 Potri.001G448400.v4.1 340 Potri.001G233950.v4.1 0 Potri.001G122700.v4.1 339 Potri.001G212900.v4.1 0 Potri.001G182400.v4.1 0 Potri.001G256600.v4.1 0 Potri.001G040500.v4.1 2 Potri.001G416900.v4.1 0 Potri.001G452600.v4.1 218 SRR26075366 completed mapping pipeline successfully