Starting /dee2/code/volunteer_pipeline.sh SRR26075367 current disk space = 3052219826176 free memory = 1466382108 SRR26075367 SRAfilesize f384b5aa18c4b9d78bd57e1e097d8cc4 SRR26075367.sra SRR26075367.sra file validated SRR26075367 is paired end SRR26075367 is conventional basespace SRR26075367 read1 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR26075367_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.66375 37.0 37.0 37.0 37.0 37.0 2 36.6365 37.0 37.0 37.0 37.0 37.0 3 36.648 37.0 37.0 37.0 37.0 37.0 4 36.7035 37.0 37.0 37.0 37.0 37.0 5 36.6355 37.0 37.0 37.0 37.0 37.0 6 36.711 37.0 37.0 37.0 37.0 37.0 7 36.591 37.0 37.0 37.0 37.0 37.0 8 36.6715 37.0 37.0 37.0 37.0 37.0 9 36.643 37.0 37.0 37.0 37.0 37.0 10-14 36.650200000000005 37.0 37.0 37.0 37.0 37.0 15-19 36.572 37.0 37.0 37.0 37.0 37.0 20-24 36.5449 37.0 37.0 37.0 37.0 37.0 25-29 36.4834 37.0 37.0 37.0 37.0 37.0 30-34 36.4733 37.0 37.0 37.0 37.0 37.0 35-39 36.4073 37.0 37.0 37.0 37.0 37.0 40-44 36.3386 37.0 37.0 37.0 37.0 37.0 45-49 36.3052 37.0 37.0 37.0 37.0 37.0 50-54 36.3111 37.0 37.0 37.0 37.0 37.0 55-59 36.1378 37.0 37.0 37.0 37.0 37.0 60-64 36.1867 37.0 37.0 37.0 37.0 37.0 65-69 36.0587 37.0 37.0 37.0 37.0 37.0 70-74 36.074 37.0 37.0 37.0 37.0 37.0 75-79 36.070299999999996 37.0 37.0 37.0 37.0 37.0 80-84 36.028 37.0 37.0 37.0 37.0 37.0 85-89 35.9764 37.0 37.0 37.0 37.0 37.0 90-94 35.9341 37.0 37.0 37.0 37.0 37.0 95-99 35.933899999999994 37.0 37.0 37.0 37.0 37.0 100-104 35.9246 37.0 37.0 37.0 37.0 37.0 105-109 35.920100000000005 37.0 37.0 37.0 37.0 37.0 110-114 35.7233 37.0 37.0 37.0 37.0 37.0 115-119 35.6409 37.0 37.0 37.0 37.0 37.0 120-124 35.7331 37.0 37.0 37.0 37.0 37.0 125-129 35.505700000000004 37.0 37.0 37.0 34.6 37.0 130-134 35.4229 37.0 37.0 37.0 34.6 37.0 135-139 35.33669999999999 37.0 37.0 37.0 34.6 37.0 140-144 35.2335 37.0 37.0 37.0 34.6 37.0 145-149 35.1428 37.0 37.0 37.0 27.4 37.0 150-151 35.07875 37.0 37.0 37.0 25.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.0 1101 2 0.0 1101 3 0.0 1101 4 0.0 1101 5 0.0 1101 6 0.0 1101 7 0.0 1101 8 0.0 1101 9 0.0 1101 10-14 0.0 1101 15-19 0.0 1101 20-24 0.0 1101 25-29 0.0 1101 30-34 0.0 1101 35-39 0.0 1101 40-44 0.0 1101 45-49 0.0 1101 50-54 0.0 1101 55-59 0.0 1101 60-64 0.0 1101 65-69 0.0 1101 70-74 0.0 1101 75-79 0.0 1101 80-84 0.0 1101 85-89 0.0 1101 90-94 0.0 1101 95-99 0.0 1101 100-104 0.0 1101 105-109 0.0 1101 110-114 0.0 1101 115-119 0.0 1101 120-124 0.0 1101 125-129 0.0 1101 130-134 0.0 1101 135-139 0.0 1101 140-144 0.0 1101 145-149 0.0 1101 150-151 0.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 20 1.0 21 1.0 22 1.0 23 1.0 24 4.0 25 11.0 26 7.0 27 7.0 28 15.0 29 28.0 30 28.0 31 45.0 32 62.0 33 93.0 34 178.0 35 456.0 36 2842.0 37 220.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 39.1793845384038 15.13635226419815 8.106079559669753 37.578183637728294 2 17.549999999999997 15.45 33.825 33.175 3 15.525 16.6 27.85 40.025 4 19.725 21.7 27.900000000000002 30.675 5 22.925 28.475 25.75 22.85 6 22.825 32.675 22.3 22.2 7 15.1 29.225 39.45 16.225 8 17.95 29.325000000000003 32.125 20.599999999999998 9 16.825000000000003 26.35 34.775 22.05 10-14 19.125 29.270000000000003 28.765 22.84 15-19 19.45 27.915 27.875 24.759999999999998 20-24 19.49 28.54 28.035 23.935000000000002 25-29 19.09 28.845 27.200000000000003 24.865000000000002 30-34 19.830000000000002 27.584999999999997 27.62 24.965 35-39 19.355 27.445000000000004 28.325 24.875 40-44 19.505 28.22 28.134999999999998 24.14 45-49 19.57 28.189999999999998 27.36 24.88 50-54 20.375 27.47 27.04 25.115 55-59 19.2 27.310000000000002 28.54 24.95 60-64 20.115 28.655 27.785 23.445 65-69 20.625 27.97 27.0 24.404999999999998 70-74 20.044999999999998 27.93 27.985 24.04 75-79 20.0 27.185 28.405 24.41 80-84 20.085 28.294999999999998 27.35 24.27 85-89 21.245 27.794999999999998 27.365000000000002 23.595 90-94 20.87 27.224999999999998 27.345000000000002 24.560000000000002 95-99 20.59 26.765 27.915 24.73 100-104 20.46 28.544999999999998 27.97 23.025000000000002 105-109 21.13 27.650000000000002 27.37 23.849999999999998 110-114 20.025000000000002 28.105000000000004 27.029999999999998 24.84 115-119 20.53 27.439999999999998 26.715 25.314999999999998 120-124 21.615000000000002 28.21 26.82 23.355 125-129 20.72 28.105000000000004 26.66 24.515 130-134 21.725 28.249999999999996 26.58 23.445 135-139 20.91 27.32 26.889999999999997 24.88 140-144 21.044999999999998 28.155 25.580000000000002 25.22 145-149 22.285 27.185 26.595000000000002 23.935000000000002 150-151 20.3875 28.15 26.75 24.712500000000002 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.5 4 0.5 5 0.0 6 0.5 7 0.5 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 2.0 17 2.0 18 0.0 19 0.0 20 0.5 21 1.0 22 0.5 23 2.0 24 2.5 25 7.0 26 11.0 27 8.5 28 8.5 29 17.0 30 25.0 31 30.0 32 36.5 33 39.0 34 45.5 35 57.0 36 64.5 37 95.5 38 127.5 39 156.5 40 164.5 41 176.5 42 215.0 43 231.0 44 268.0 45 265.0 46 283.5 47 291.5 48 237.5 49 218.5 50 182.0 51 136.5 52 96.5 53 86.0 54 95.0 55 79.0 56 55.5 57 35.5 58 29.5 59 19.0 60 10.0 61 15.5 62 11.5 63 8.0 64 9.0 65 7.5 66 8.0 67 8.0 68 5.5 69 3.0 70 2.0 71 1.0 72 0.0 73 1.0 74 1.0 75 0.0 76 0.0 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.5 96 0.5 97 0.5 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.075 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150-151 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 55.50000000000001 #Duplication Level Percentage of deduplicated Percentage of total 1 58.6036036036036 32.525 2 21.71171171171171 24.099999999999998 3 10.63063063063063 17.7 4 4.234234234234234 9.4 5 2.2072072072072073 6.125 6 1.4414414414414414 4.8 7 0.5405405405405406 2.1 8 0.3153153153153153 1.4000000000000001 9 0.09009009009009009 0.44999999999999996 >10 0.22522522522522523 1.4000000000000001 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CTACTGCCTGATCGATGGAGTTTCTCAAAGAGAGTAGGTTTCTTCTCCTC 13 0.325 No Hit CCCATTCTCAATCTCCTTGTATGGGAACGAATCCGAGAGAAGCTCATCAC 11 0.27499999999999997 No Hit CTTCGCAATCAGTCAAGCTAATTTTACTCACTTCGGTTCCCAGGCGGTTA 11 0.27499999999999997 No Hit CCCCAATAAACACAATCACAGAGAGCGTAGCCACTGCAGTGACAGAATAA 11 0.27499999999999997 No Hit GATCGGAAGAGCACACGTCTGAACTCCAGTCACACGGTCTTATCTCGTAT 10 0.25 TruSeq Adapter, Index 5 (97% over 38bp) CCGGTCAAGTCTTCCAGGACGCAGAAGTGCAGGGTCCAAAGTGTCAGCCC 9 0.22499999999999998 No Hit CACCAGTAATCACGAGCTGCCCTTCCCTTCACCACCATTTCTACCGAGCC 9 0.22499999999999998 No Hit TGGTCCTGTTAGAGTGGCTGTTACTAAATTGTCACCCCCAAAGACTGCCC 8 0.2 No Hit CCTTCATCTGGTCAACAAACCCTTCCTTGCGCTGACCTTGTGCGCCGTCC 8 0.2 No Hit GTCTGCCAGGGTGCGCCCATCTTCCAGCTGCTTTCCAGCAAAGATCAACC 8 0.2 No Hit CTTGGCTTATGTTGACACATGATAAAACCTCTCGTCCTATGACTTTGGTT 8 0.2 No Hit CCTTGATCAATCGTCTTGCCATCATTAGTTGGGCCTTCTGCAGGGGAAGG 8 0.2 No Hit GATCGCAGTAGCTCATTCCCAATTCAGTTCTATTGTTCCCGCCCTTCTAC 8 0.2 No Hit ACGGCAATACCGCAGTCAGACTTCTTCAAGGAGGGAGCGTCGTTGACACC 8 0.2 No Hit GGGTTCTTCACAATGACAATCATACCAGGCAACAACAGTGGCAATTCTGG 7 0.17500000000000002 No Hit CCCACATTAAAAGAATTGCGTGTGCTCGAAAACAAAATCAAGATTCAACG 7 0.17500000000000002 No Hit CACCACCTTTCACGGGTTGAATAATGCCCGCATTAACAGCCGTCTCGCGC 7 0.17500000000000002 No Hit CGCGTTCTGGTCACAGGTTCCAACGCTTCAACAGTGACAACACTATTGCC 7 0.17500000000000002 No Hit GTACTGAGTTATTAGGAGAGGAAGCGTTTGAATCAGATATGTAAGCTGAT 7 0.17500000000000002 No Hit GGTTGAAGGAACAGTGGGGACGACATTTTGTTAATTTCTACCTTAGATCC 7 0.17500000000000002 No Hit CTCCAGTTTCCTTAACAGTAACACTAGGGCTCAGTGAGCGCATTGATCCA 7 0.17500000000000002 No Hit GCATCGATATCTTTGGTAACAGTTATCATGAAATCCATGTACTTGTCTGG 7 0.17500000000000002 No Hit CTCCTCAACAACCGGCTCTGGTGTTTCCTTAGGTGTCTCCTCCTCTGTTT 7 0.17500000000000002 No Hit GTCCAAACCAGCTCTCCCAGTCAAAAGCTGTAACAGTATAATTCCAAAAG 7 0.17500000000000002 No Hit CCGCTTCGTTTGCTCATCCGATTGCATCAGCTTCATCTCTTCCATCACAT 7 0.17500000000000002 No Hit GCCACAACCAATTTATCAACTCTACCAAGGTCCAGTCAGTGTCCTTCGCA 7 0.17500000000000002 No Hit TTTCTTTGTTGGCGCCATTTGTGACCATCAACAGTGAAAATTCCATCACC 6 0.15 No Hit AGCTGGAATTGCTGCCCCTAATAGAGACCACCATGGGTCCACCATTGCTC 6 0.15 No Hit CTAGACATTTTAACTTGACATGCTGTAAGTTTGTTTTGACAGAAAACTCT 6 0.15 No Hit CCTGCATACACTAAAAAAGGAGATCAAATATTTTACATCAACAATATTGT 6 0.15 No Hit CTATAAATTTCATTGCAGCTCATATCCACATATAAGTTGATCACCCATGC 6 0.15 No Hit CTTCCTTTTTCACTTTATGTGTGTATGTATGCCCGCATGGAGTCATGTTT 6 0.15 No Hit ACCGCGCCCTCTTCTCCACCTGCTATCGGGCACCTCCCATGATCACTACT 6 0.15 No Hit ATCCCAAGAAAAGCCATTTTGTTGCAGAATAACCTTCAGATCACTATAGT 6 0.15 No Hit TGGTGATCCACAGTAAAATGGTGGTGATGATGCCACCTGGAAACCGGGTT 6 0.15 No Hit CTCCAATTCACGGTGGTGATCACGAGGCAATTCAAAAAGGCCCCTTTTCA 6 0.15 No Hit GCACTAAGAAGTGCTTGGATGCTCAAAAGCACAGTTCGTATCTGAAGAGC 6 0.15 No Hit CCACCGTGGGCTTTTTCTACGGCAAGATTGACACCCGCTTTTATTACATT 6 0.15 No Hit GATTTGGTTAAGTTGTTGACAAGAAATAATCATCGAGTTTTAACCATAAC 6 0.15 No Hit ATAGCAGTAATACTAATAATCATAATATGCCTTGCATAAAATCCAACAAA 6 0.15 No Hit GCACCAGGACCGCACACCACCATGGCTATATATGCAGATGAATTGATGAC 6 0.15 No Hit CAGTCGTAAGTGTCCACTGTTGGCAAGTCCACCAAACATAAGAATTTTTG 6 0.15 No Hit CAATTGGCGAATTGGAAGAAACAAAGTTCTTCAGCACCACTCTAACCCCA 6 0.15 No Hit CCGTTAACCTCTTCTTCTTGAGATACCTTGTTGTTGAAGGCCTTGTGAAC 6 0.15 No Hit GTTCCTAAAAAGTCCTGGAACTGCAGAGGAGGCCCTCAGGAACTGTGGAG 6 0.15 No Hit GTTTCAAAACCGTGCCCCGACAAAAATGCATTGGCCCCAGTCCCTGCCTG 6 0.15 No Hit GCGTGATGCAGCTTCATAAAGAATGTTGTGAAGGGTCCTGCGCTCTCTCT 6 0.15 No Hit CATTTCTCTTCTCCTCATCGGGCAGATTCTCATAATAAGCTCCAGCAGCC 6 0.15 No Hit GTAGCATCAAAGTAAACTGTCAGAAGATTTTTATTTTCTTGATAAAGACT 6 0.15 No Hit CCTAGATCTTTAACTAATTTCCCCCAAAATGTGTGGACGATTTGGCAATC 6 0.15 No Hit TCCTGTGGAACTTCGGTGCTGCTGTGGAGTGGAGGGAAGGCAAGAGAGTG 6 0.15 No Hit GGACAAAGAAAAGAAGACCACTGAATATCATACAACTGCACTATTTGATT 6 0.15 No Hit GATCGATAGGCCACGCTTTCACGGTTCGTATTCGTACTGGAAATCAGAAT 6 0.15 No Hit CAACAAAGAAGCATCCAAAGCCTCTTCCACTTTTCCAGCTTTCACCAACG 6 0.15 No Hit CCCTAGTGTGTGTGCACCAGACAATGCAACTATTTCCTTGTCATTTAATC 6 0.15 No Hit GTGTGCTTGATCTTCTTGGGCTTGGTGTAGGTCTTCTTCTTTCTCTTCTT 6 0.15 No Hit TTTTTTTTTTTTATCATAGCAACACTACCATTTTAATTATACATGAAAGA 6 0.15 No Hit ATTCTCTGGACTGAGCTTGATCCTTTTTCGGACCACGTACACGAATTGGC 6 0.15 No Hit GGGATAATAGCCTTGAGAAACATGTCCTCTATACCTACAGTGATACTTTG 5 0.125 No Hit GAGTCCAAAGACATAGCTGCGGTGATGGGGGCGGAGAGGGATGAGGGATA 5 0.125 No Hit GACCACCTGGACTTTCCTTACTTTTCTCTGGCACTTGCTGTCTAGCAACA 5 0.125 No Hit GCTTCGTTCACGGTGATGTTACGGCCATCGAGGTCCTGACCGTTCATTCC 5 0.125 No Hit GCCATGTAAACTACCTGAATTATAGCAGTGAAAACTGCAAAAACCACATA 5 0.125 No Hit CCCCAGCAATGGCACCAATCATAGTCATGTCTTTATTCTTGTTGCTGGCA 5 0.125 No Hit AGCTTTTTCGTACACGTTCACTTGCATCACATACACAAGTGCTCTCTGAA 5 0.125 No Hit TCTCCTTCTTCCTCATCCTCATATTCTCCTTCCTCATCAGCGGTTGCATC 5 0.125 No Hit GCAATCATCAGTACGGACACCATTTGACACATCAGCTTGATCCCTCATAT 5 0.125 No Hit GCTTCATATTTGGCTCGGTCAGTCTTGTACATATGGGCAATCTTAGAAAT 5 0.125 No Hit TTTTTTTAAAACAAAGCTGAGCACACTTGAAATGCATTCAATTCCTCTTG 5 0.125 No Hit CTCTTATCTTAGAAGGGGTAGTGGGAACTCCAGGCTATGAATGAAAGGAC 5 0.125 No Hit GTTTTTCTATAGCATGAGCTCTGGCAAATCATTAGAATAAGAAAGCCCTT 5 0.125 No Hit GCCCCCACCATAACCTCCCTTAGTAAGAATTATGTCTAGCAGCTCTGCCC 5 0.125 No Hit CTCGTACTAGGTTGGATTACTATTGCGACACTGTCATCAGTAGGGTAAAA 5 0.125 No Hit GCCCGACTACTTCTGACCCTCTTCAGCAACAGCTTCAAAGGTCTCACCAG 5 0.125 No Hit CTCCTGGTACTGGGGCTCTAACACCATAAGCCATGTGTGCTGGTGCTGAA 5 0.125 No Hit CTAACAACCTGGATTTCCCGATTACTGGGTCCACGACAACCGGAATCAAA 5 0.125 No Hit GATCGGAAGAGCACACGTCTGAACTCCAGTCACACGGTCTTATCGCGTAT 5 0.125 TruSeq Adapter, Index 5 (97% over 38bp) CTTCAAAAACATCTCTCACTCTCAGCAGTTGAAGCCTTTGCAAATAGAGA 5 0.125 No Hit GCCAAAGTAACCAGGAGGGAGTTGAGGTTGCCATCTTGACCGACCATCTG 5 0.125 No Hit ACTCTCTTGGACTTTCAAGATCATCCACTCCACTATCAAAATTTTCACTA 5 0.125 No Hit TTATTGCCTTCTGGAAAATCGCTGGGTTGCTTGTTACGCCTCTAACTCCA 5 0.125 No Hit GTCCTAACTCGTTCCTTGAGACCTTCATTCTCACTCTCAACATCCCCACT 5 0.125 No Hit CAACAACAACGAAAAAGGCAGGTACATATATATGGCTACAGAGGGAAGAT 5 0.125 No Hit CGAGTCTTTGCTGTTATTTTCTGCACCCGAAGCCGAACCTGTTTTCTCAT 5 0.125 No Hit TTCAAGCTTTTTCTATGGATACAACTACTTATTCAATGGACTGTCCCCTG 5 0.125 No Hit ACTTGGGGGAGGGTAAAATGTAATATTACAACAAAGAGATTCTGTTGTAA 5 0.125 No Hit ACGACCATACAGCTTTAGAGCTTCCAGGAATCTCTGGTGCTCTTCATCTG 5 0.125 No Hit AGTTACTAGGATCACTGTCGTAGACATCATTAAGATCTTTTATGAGTTCA 5 0.125 No Hit CCTCCTCTTCCTCTCCACCATAACATTCCCCACTCCCACACCTACCAGAT 5 0.125 No Hit GTCCAATACAAGAGCAGACGCTCTAAATTGCATGCTCAGTCAAACTTCTT 5 0.125 No Hit CAACAAAGAAAGAACCAAGTCACCAAAATCAACGTATTATTAGAGTCTAT 5 0.125 No Hit CTGAACTACAACACTGAAACAAATTGCTTCAAGACCTGTCTGCCACAAGT 5 0.125 No Hit TTCTCTTCTTCTTTCTTCCCCAGGAAATCAAACAACCCGCGATCCTTGGT 5 0.125 No Hit GTCCTACGTAAGCCAAGGTATTGCCACTAGGCGACCACTTCACACCAAAA 5 0.125 No Hit AGTTGTTTTAGCTTGTAAGACCTTCTTTTGAGGAGTTCTTGGCGCGTGAT 5 0.125 No Hit TCCACATGCTTAACCACATTCACGGTCAATTTTCAAGCCCTTCCAATGAG 5 0.125 No Hit CACCTCGATGCCAGGGAAGGAAGGGATGCAGTAAACTGAAAAGGAGAGAT 5 0.125 No Hit CTCTGTTAGTGCTGTGCTCACAGTACCCATTCTCTCTTCAAGAGCTAATA 5 0.125 No Hit CCCTCAATACATCTCTCCACAGCACTCCTCGCGAATCGGGCCACATATAG 5 0.125 No Hit AGCAGGCAATTATGACTTCACCATGTCGTACAAGGCTGGAATTGTAGACG 5 0.125 No Hit CTCACATCAATTTACCTTTTGTACTCAATGAACCAAATCATTATTGCAAA 5 0.125 No Hit CAGGGTGTTCACCAGGCTCATACAGAATTCCATTGCTAGAGACCATGTCA 5 0.125 No Hit CCGACTTTTCAGAATATCGAAAAAAGTTTTCTGAGCAGCATCCCTCAGTG 5 0.125 No Hit CCTGGGCAGACCCAGTGAGAGCGCTGAGAGTGGACTTTAACTGGCTTGCA 5 0.125 No Hit GCCTGGGAATGTAATGGTGGTTATTCACACTGCTCCCCGGATACCCAGCA 5 0.125 No Hit CCCCATGCCACTGATTACCATGATCCCCTCTCCACTGCTTGTTTGCTGGA 5 0.125 No Hit GGCATCGAACAAGTTGGAGAAAGAACAAGATTTCTGGGACATGAGATTAT 5 0.125 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0 0.0 0.0 0.0 0.0 64-65 0.0 0.0 0.0 0.0 0.0 66-67 0.037500000000000006 0.0 0.0 0.0 0.0 68-69 0.05 0.0 0.0 0.0 0.0 70-71 0.0625 0.0 0.0 0.0 0.0 72-73 0.075 0.0 0.0 0.0 0.0 74-75 0.1 0.0 0.0 0.0 0.0 76-77 0.125 0.0 0.0 0.0 0.0 78-79 0.175 0.0 0.0 0.0 0.0 80-81 0.2 0.0 0.0 0.0 0.0 82-83 0.2 0.0 0.0 0.0 0.0 84-85 0.225 0.0 0.0 0.0 0.0 86-87 0.225 0.0 0.0 0.0 0.0 88-89 0.275 0.0 0.0 0.0 0.0 90-91 0.375 0.0 0.0 0.0 0.0 92-93 0.44999999999999996 0.0 0.0 0.0 0.0 94-95 0.5375 0.0 0.0 0.0 0.0 96-97 0.7124999999999999 0.0 0.0 0.0 0.0 98-99 0.9125 0.0 0.0 0.0 0.0 100-101 1.0875 0.0 0.0 0.0 0.0 102-103 1.2875 0.0 0.0 0.0 0.0 104-105 1.5125000000000002 0.0 0.0 0.0 0.0 106-107 1.7 0.0 0.0 0.0 0.0 108-109 1.8375 0.0 0.0 0.0 0.0 110-111 2.2249999999999996 0.0 0.0 0.0 0.0 112-113 2.8 0.0 0.0 0.0 0.0 114-115 3.2 0.0 0.0 0.0 0.0 116-117 3.7375 0.0 0.0 0.0 0.0 118-119 4.3 0.0 0.0 0.0 0.0 120-121 4.8 0.0 0.0 0.0 0.0 122-123 5.325 0.0 0.0 0.0 0.0 124-125 6.1 0.0 0.0 0.0 0.0 126-127 7.137499999999999 0.0 0.0 0.0 0.0 128-129 7.8625 0.0 0.0 0.0 0.0 130-131 8.5625 0.0 0.0 0.0 0.0 132-133 9.25 0.0 0.0 0.0 0.0 134-135 9.899999999999999 0.0 0.0 0.0 0.0 136-137 10.675 0.0 0.0 0.0 0.0 138-139 11.475 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATCTGGT 10 0.006830828 145.0 6 >>END_MODULE SRR26075367 read2 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR26075367_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.2845 37.0 37.0 37.0 37.0 37.0 2 36.363 37.0 37.0 37.0 37.0 37.0 3 36.409 37.0 37.0 37.0 37.0 37.0 4 36.345 37.0 37.0 37.0 37.0 37.0 5 36.3885 37.0 37.0 37.0 37.0 37.0 6 36.28 37.0 37.0 37.0 37.0 37.0 7 36.2775 37.0 37.0 37.0 37.0 37.0 8 36.2925 37.0 37.0 37.0 37.0 37.0 9 36.359 37.0 37.0 37.0 37.0 37.0 10-14 36.232299999999995 37.0 37.0 37.0 37.0 37.0 15-19 36.2353 37.0 37.0 37.0 37.0 37.0 20-24 36.145900000000005 37.0 37.0 37.0 37.0 37.0 25-29 36.0573 37.0 37.0 37.0 37.0 37.0 30-34 35.9688 37.0 37.0 37.0 37.0 37.0 35-39 35.8972 37.0 37.0 37.0 37.0 37.0 40-44 35.9165 37.0 37.0 37.0 37.0 37.0 45-49 35.854400000000005 37.0 37.0 37.0 37.0 37.0 50-54 35.694300000000005 37.0 37.0 37.0 37.0 37.0 55-59 35.758799999999994 37.0 37.0 37.0 37.0 37.0 60-64 35.7861 37.0 37.0 37.0 37.0 37.0 65-69 35.6402 37.0 37.0 37.0 37.0 37.0 70-74 35.6093 37.0 37.0 37.0 37.0 37.0 75-79 35.452299999999994 37.0 37.0 37.0 37.0 37.0 80-84 35.5441 37.0 37.0 37.0 37.0 37.0 85-89 35.5668 37.0 37.0 37.0 37.0 37.0 90-94 35.4125 37.0 37.0 37.0 37.0 37.0 95-99 35.5285 37.0 37.0 37.0 37.0 37.0 100-104 35.4234 37.0 37.0 37.0 37.0 37.0 105-109 35.417699999999996 37.0 37.0 37.0 34.6 37.0 110-114 35.2681 37.0 37.0 37.0 34.6 37.0 115-119 35.2997 37.0 37.0 37.0 37.0 37.0 120-124 35.194599999999994 37.0 37.0 37.0 32.2 37.0 125-129 35.1569 37.0 37.0 37.0 29.8 37.0 130-134 35.115700000000004 37.0 37.0 37.0 29.8 37.0 135-139 34.9818 37.0 37.0 37.0 25.0 37.0 140-144 35.019099999999995 37.0 37.0 37.0 27.4 37.0 145-149 34.8515 37.0 37.0 37.0 25.0 37.0 150-151 34.60325 37.0 37.0 37.0 25.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.0 1101 2 0.0 1101 3 0.0 1101 4 0.0 1101 5 0.0 1101 6 0.0 1101 7 0.0 1101 8 0.0 1101 9 0.0 1101 10-14 0.0 1101 15-19 0.0 1101 20-24 0.0 1101 25-29 0.0 1101 30-34 0.0 1101 35-39 0.0 1101 40-44 0.0 1101 45-49 0.0 1101 50-54 0.0 1101 55-59 0.0 1101 60-64 0.0 1101 65-69 0.0 1101 70-74 0.0 1101 75-79 0.0 1101 80-84 0.0 1101 85-89 0.0 1101 90-94 0.0 1101 95-99 0.0 1101 100-104 0.0 1101 105-109 0.0 1101 110-114 0.0 1101 115-119 0.0 1101 120-124 0.0 1101 125-129 0.0 1101 130-134 0.0 1101 135-139 0.0 1101 140-144 0.0 1101 145-149 0.0 1101 150-151 0.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 13 1.0 14 7.0 15 5.0 16 2.0 17 9.0 18 5.0 19 9.0 20 7.0 21 12.0 22 15.0 23 7.0 24 11.0 25 18.0 26 14.0 27 15.0 28 12.0 29 21.0 30 22.0 31 37.0 32 40.0 33 95.0 34 183.0 35 610.0 36 2637.0 37 206.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.15 22.95 12.950000000000001 20.95 2 30.075000000000003 27.725 27.450000000000003 14.75 3 25.4 29.075 28.4 17.125 4 23.65 33.800000000000004 23.375 19.175 5 28.825 33.074999999999996 22.825 15.275 6 26.174999999999997 36.85 20.1 16.875 7 23.7 20.7 36.65 18.95 8 24.175 23.9 27.950000000000003 23.974999999999998 9 23.9 26.224999999999998 27.175 22.7 10-14 24.88 29.304999999999996 25.380000000000003 20.435 15-19 25.2 28.51 26.1 20.19 20-24 24.825 28.335 25.955000000000002 20.885 25-29 26.150000000000002 28.465 25.650000000000002 19.735 30-34 25.345000000000002 28.055000000000003 26.915 19.685 35-39 24.985 28.044999999999998 26.025 20.945 40-44 24.21 28.33 26.955000000000002 20.505000000000003 45-49 24.005000000000003 28.599999999999998 26.82 20.575 50-54 24.33 27.810000000000002 27.815 20.044999999999998 55-59 24.515 27.98 26.950000000000003 20.555 60-64 25.765 27.905 26.63 19.7 65-69 25.735000000000003 27.46 27.465 19.34 70-74 23.695 28.575 26.69 21.04 75-79 24.425 28.910000000000004 26.740000000000002 19.925 80-84 24.59 27.97 26.85 20.59 85-89 25.77 27.705000000000002 26.55 19.975 90-94 25.025 27.625 27.155 20.195 95-99 25.11 28.134999999999998 27.02 19.735 100-104 25.185000000000002 28.294999999999998 26.090000000000003 20.43 105-109 25.365 27.98 27.155 19.5 110-114 25.055 28.925 26.06 19.96 115-119 26.150000000000002 28.87 26.035000000000004 18.945 120-124 25.919999999999998 29.37 25.355 19.355 125-129 26.035000000000004 28.74 26.465 18.759999999999998 130-134 26.82 28.67 26.19 18.32 135-139 26.334999999999997 28.43 26.25 18.985 140-144 27.355 28.865000000000002 25.2 18.58 145-149 27.255000000000003 28.345 25.615 18.785 150-151 26.237500000000004 28.449999999999996 26.087500000000002 19.225 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.5 9 2.0 10 1.5 11 0.5 12 2.0 13 2.0 14 1.5 15 2.0 16 1.5 17 1.0 18 1.0 19 1.0 20 1.5 21 2.0 22 2.5 23 3.0 24 4.0 25 4.0 26 2.0 27 0.5 28 2.0 29 13.5 30 16.5 31 12.5 32 15.0 33 22.5 34 33.5 35 47.5 36 65.0 37 72.0 38 112.0 39 147.5 40 182.0 41 240.5 42 264.0 43 273.0 44 256.5 45 257.0 46 266.0 47 261.5 48 253.5 49 207.5 50 190.5 51 163.5 52 112.5 53 87.5 54 68.5 55 63.5 56 46.0 57 26.5 58 27.0 59 28.5 60 19.0 61 8.5 62 12.5 63 13.5 64 6.5 65 3.5 66 3.5 67 2.0 68 0.5 69 1.0 70 2.0 71 2.0 72 1.5 73 1.5 74 1.0 75 1.5 76 1.0 77 0.0 78 1.5 79 2.0 80 1.5 81 2.0 82 1.0 83 1.0 84 3.0 85 3.0 86 2.5 87 2.0 88 0.5 89 1.0 90 2.0 91 1.0 92 0.5 93 1.5 94 2.5 95 2.5 96 2.0 97 1.5 98 1.0 99 2.0 100 6.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150-151 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 56.875 #Duplication Level Percentage of deduplicated Percentage of total 1 61.27472527472527 34.849999999999994 2 20.35164835164835 23.150000000000002 3 9.626373626373626 16.425 4 4.175824175824175 9.5 5 2.0219780219780223 5.75 6 1.3626373626373627 4.65 7 0.6593406593406593 2.625 8 0.3076923076923077 1.4000000000000001 9 0.04395604395604395 0.22499999999999998 >10 0.1758241758241758 1.425 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG 23 0.575 No Hit ATGAGACCAAAGTTGGTGAAGAGAGTGGTGCTGTTGAGACCAAGGATCGC 13 0.325 No Hit CCAATTTCAACCCCCTGGCTCTCAACAATAAAATACAACCATGTCTAGCT 11 0.27499999999999997 No Hit GGGGCGTTGCCTTCTTATCCTCAGTGATGGCGTCAACTACTGGGATTGTG 10 0.25 No Hit GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGT 9 0.22499999999999998 No Hit CAGGGACTTGCATTTATTCTTGCTGGAGGATCTGTTGCACAGAAAGTTCT 8 0.2 No Hit GTCTACAACTCTGAGCGTCTCATCCACGGTGGTCTCTCTGGAACTACTCA 8 0.2 No Hit GAAATGCTATCAATGAAATTGTGGGAATTCTTTGCGGAAATGAAACAAAC 8 0.2 No Hit TGGCTGTTTTTTTTAGGCACAGCTCTAAATGCCTAGAGCATCTGTTATGG 8 0.2 No Hit GTTTGCTTCAATAAGTTCAGCCATGGGCTTGAACCATTTCACTGTCAATC 8 0.2 No Hit AGTCAACCCTTCACTTGGTGCTGCGTCTCCGTGGAGGAATGCAGATTTTT 8 0.2 No Hit CAAAAGACCACTACTCTTCCTCTCTATAATTTTCTAGGGTTTAGCAATGT 8 0.2 No Hit GTGCAGATCAGCCTAACCAACTCCGTATACTGGAATGCTCACACCTTTGC 7 0.17500000000000002 No Hit AAGCTCTGAAGAAAAGTGTTTCAACACCCTTCATGAATACTTCTTCAAAA 7 0.17500000000000002 No Hit CGGAAGATGGTTTCGAAAAGGAGCTGATGGGATTAACAGGGGGCTTTCCT 7 0.17500000000000002 No Hit GAGCGTTTGATCTTTGAACCTTTTATTGAGACTGATGAGATTGTTGTCTC 7 0.17500000000000002 No Hit TCTTACTCTCCATTCTCTCTCTTCTCACACAACACTCAACTCCGAAACCA 7 0.17500000000000002 No Hit GTAGTGGATGAAGAAAAGAACACACTGGCTCTCACTGGCCTGGAAGGAGA 7 0.17500000000000002 No Hit CAGTCGTAAGTTTCTGGCGGAACTCCCCCTCTACCTAGATCATGGCATAG 7 0.17500000000000002 No Hit ATTGAGACCGCCACAGAAGAAGCAGTCGCTGCAGCACCAGAGGCAGTAGC 7 0.17500000000000002 No Hit GTTTGCCGGCCATTTAAAGTTAGAGTTGAACAAGTTTTGCAGTCACAACC 7 0.17500000000000002 No Hit ATTTGAAATGCAGAAAAATCAGTTCTTAGAGACAATGAAAGCGCAGTGGG 7 0.17500000000000002 No Hit GATCGGGGCAGCCACCAGATCTCAAGAAGTACATGGATAAGAAGCTTCAA 7 0.17500000000000002 No Hit TTGACTTCGAATAAGAGAGACCTGAAAAACCATAATTAAAAACCCTTGGA 7 0.17500000000000002 No Hit AGAGAAGTTCAGCGTATTCTGATGGAGCTCCTGAATCAGATGGATGGGTT 7 0.17500000000000002 No Hit ATTATCAGTTCTGAAGATGAGTTCCATGAGATTGCATGCTCTTCCCATCA 7 0.17500000000000002 No Hit AGAAGAAAGAAATACACAATGGCAGGAATCATGCACAAGATTGAGGAGAC 7 0.17500000000000002 No Hit GGGTTGTCTCTTGTCTTCAACAAGTTTCAATAAAGTAGCTAGTATATATT 6 0.15 No Hit AGAAGGCATAAGCTAGCAATGAAGTTCTTGTTCCAGTGCCCTTGCTGCTC 6 0.15 No Hit GTTGAGCTTATGCTGGACCAAATGGGAAGAGGCAACAAGACTGATAATAC 6 0.15 No Hit ACTCTGACAAACAGATGGTGTTCTGTTCGAAATGTGGAACTTCTAATCAT 6 0.15 No Hit GTTTGGCTGGGGCGGCACATCTGTTAAAAGATAACGCAGGTGTCCTAAGA 6 0.15 No Hit CTCAGCTCCTGAGGAATGCCCAGAAGAGGGGAGGCTTCCTGCTGCTGGCC 6 0.15 No Hit ATGACCCGTAGTGAAGCATAGTCTGCAGATGACTCTGTATACCTTTTGTG 6 0.15 No Hit AGGAGATGACATGGGAGTACTGGGATTATCATTGCGGTCTAATTGGTTAC 6 0.15 No Hit GCTCTGCCTACCAAACTAGGCAGCGGCAAGGCTCGAACACCACGTCCGGT 6 0.15 No Hit GGACAATCGCATCTTACTACAAGAGTGGCTGGAACTCATGGGTACTTAGC 6 0.15 No Hit CCGAAGCCAAGGAGATTGGGACTTTTAAATCCTCCTCTTAATGAACAAAT 6 0.15 No Hit TGACCATTTAGACTGATGGTTTCTGTTTTTTGGTGTCAATTGTACTGGAG 6 0.15 No Hit AAGCAAATGACCCAGTTGATTTGTTCCTCCTGCCTGGACTTGCATTTGAC 6 0.15 No Hit AATTGAACTGGGTTCTTCAAGAAAAAGTGAAGAGCTTATACGTTGAGACT 6 0.15 No Hit GAAACGCTTTCCCTCCCTCAAAAGGCGACCAGCTTTCAATACTAGTTGTT 6 0.15 No Hit GTAGCCATAATCGGGGACCTGTGCAGAGGGCTTTTCTGGGAAGTGTCAGC 6 0.15 No Hit CACCATTTCCAGCTCAGAGGAAATCTTCCTGGTGACAAATTCCAAGAGCT 6 0.15 No Hit GTCCGGATGGACATGTAGCTTCTCTGTTCCCTGGGCATCCACTTCTCGAG 6 0.15 No Hit ACCAAAGTCCCTTTGGAGTGTTAAAATTGTTGATCTAAGCTACCTCTTCC 6 0.15 No Hit GTCGCACCCTCGCTGACTACAACATCCAGAAGGAGTCCACTCTCCACTTG 6 0.15 No Hit AGGATATTCAAATTCAGAGAGGAAAGCCATTGTACCTGAATGAGGAGCGC 6 0.15 No Hit TCCTTCCTTCCGTCGGGATCCGAATTCTGATTTCTTCTTCTCAATCTCGA 6 0.15 No Hit AGAAACATCATCACAGGGCTCTTTCCAATGGCAGCCCTGTCCCCTCTCCT 6 0.15 No Hit GACTAATCTTGCTGGGAAATATAAGACTTACAGGCTGCGCGCCCCTTTCA 6 0.15 No Hit AATCTGTGGAGCAAGCCTATGGACTGAATGAGAGTGATAGGGAAAAGAGT 6 0.15 No Hit AGCTTCTGAGGATTACATCCAGCGATGCTTTGAGAAAGATAGGAAATCTA 6 0.15 No Hit GAGATGCTCTGATAGAACTCTTTGGAAAGGTCAGGGATGAATGGATGGAT 6 0.15 No Hit ATTATTTCTGCCTGAAGAATATCCAATGGCTGCGCCTAAGGTTCGATTTC 6 0.15 No Hit AGGTAACGGCGTTGCCCACAGCCGACAGGAAGGTGAAGAGGGGAGGAAGC 6 0.15 No Hit TTGCCTGTCTTTGGGTCGATCCGAAAGAGAGGAGCTCTTCTGCGCAATCA 6 0.15 No Hit AGACTAAGAAGTTTGGTGGACGTGGTGCCAGAGCCAGGTTCCAGAAATCT 6 0.15 No Hit GTGTGGTGGCAGATACCTTTCTTGAAACCATCAATTCACACACGCAGCCT 5 0.125 No Hit TGGACATTGTGGTCCATGCACAACAAATTCCAAACATGGCATTGGTGTTC 5 0.125 No Hit GAGGTTGGTGAGAGAGAGGAAATGATTGGGTTTATGTCACGAAATGCTGT 5 0.125 No Hit CTGTGGACAAAAGGACTGCTTTGACTTACATTGATGCTGATGGAAACTGG 5 0.125 No Hit AAAGAGGGCATCCAAAATCAGGAAGCTTTTTAACCTCTCTAAGGAGGATG 5 0.125 No Hit TATTAAGCCAACATCAATAGTGAGTTACAACCATCTGGGAAACAATGATG 5 0.125 No Hit AAACCTGGGGAGGAACCAACAGAGCCGTACACCTATTTGCAAATTGATCC 5 0.125 No Hit GTCACATATAATTGTTACATTTTAGAATTGTTCCTTTTAAAATATCATTT 5 0.125 No Hit AGAGGATTGAACAAGATGAAGAACTAACATACGAGCAATTGCTTGTTTTG 5 0.125 No Hit AGCAAACTACAGCGCACAATTCCCGGTATCGATACCATAATGGAGAAAGT 5 0.125 No Hit GTTGGAAGCTTTGCGCTAGTTAGTGTTGATAATGGCGAGAACGACCAGCA 5 0.125 No Hit ACTACAACAACAAGAGAAGATATCACAACCCCCTAGTTAATCTTCCATGA 5 0.125 No Hit GCTTGAAGTTGAAGGAGTGGATTCTTTCAAGAAGAGCTTTGATAGTCTGC 5 0.125 No Hit TGCCTGGATAGTGATTTTGTGTACTACTAGGAAATGTGGGAATCCAAACT 5 0.125 No Hit GCACGAATCTGAACTCCTTTGGTAAAGCTTGTGATTTCGGTACCAATGGC 5 0.125 No Hit GGGTTATTTCTGGCACTCCTCAAACCAGAAAACTGCAAGATATTCTTCCA 5 0.125 No Hit AGGACATGGAATCTATTGTGGAGACCATGATGCAACAGCTTTTATCTAAG 5 0.125 No Hit GGTTTACAAACTTGATTGGTTGTCTGTATGGAAGTTTGTTGTTCCACATA 5 0.125 No Hit GATGGAAAATGTCGAGTGTTTTCCACTTTTATTAAAGGTGTTGATACAAG 5 0.125 No Hit TTGTCTGTCTTTGGGTCGATCCGAAAGAGAGGAGCTCTTCTGCGCAATCA 5 0.125 No Hit GAAACACAGCTCACTACGGGATGCTGTCTATGTACCTGCAACTCATGCTG 5 0.125 No Hit CAAGACTGAGAACCTTCATCAACAGGCACAAGACTTCCGCAGTCAAGGGA 5 0.125 No Hit GGAAGACACAATCCTTACCTCAGAGAAACCCTCAAATCCTTCCCCTCTCG 5 0.125 No Hit TACAATTAAGATCTGGTTTTCAAGTATGTAAATTAGAATATTGTGCCCTG 5 0.125 No Hit CAACAACCCCACAACCTAACATCACTCAAAACCCTAACCCAACCACTAAT 5 0.125 No Hit GGAATTTGAAGCTAGAGGTGTCAGAAAAGTTACCACAGGGATAACTGGCT 5 0.125 No Hit TTTTTTTTAAACCTAGCCTGGAATAACAGAGTTCATATGTACTGGGTGAA 5 0.125 No Hit GAGGCAGCCTAAATGGCTATGAAGCTGCAAAAATGGCGAAGTCTATCTTC 5 0.125 No Hit GCCAGCCTATAGGAGGTCCCCTTGTTTCAGCAGTTGGCACCCCAGTGAAT 5 0.125 No Hit TTTTAGTCTCCATTCTTCGTGGTCCCTTGTTCTCCCCCCCTCTCTCTCTA 5 0.125 No Hit AGTGTGGACAAGGTGGGTCTGAATGTTGTCTTTTCTGTTCTTATTACAGC 5 0.125 No Hit GACTTCCCCAGTTACATAGAATGCCAAGAGGAGGTCGATAAAGCATACCA 5 0.125 No Hit CCTACTTTGTTGAGTGGATTCCAAACAATGTGAAATCAACTGTGTGCGAT 5 0.125 No Hit AATATGGAATGGAGAGAATTCGTGGCACTCATGACTGGAAGAATGCCATG 5 0.125 No Hit AGAAGGAAGAGGTTGTTGATGATGATGATGATGTCCAGTGGCAAACTGAT 5 0.125 No Hit CCAAAGTGCAACTAAAACCCAAAAAAAATGTCTAAGCTTACAACCCTCTT 5 0.125 No Hit GTTACAAGCGCAAATCACTCGAAGCAGAAGCTTACTCATTTTAATTACTC 5 0.125 No Hit GATATTTCCGGCCAGAGTGTTTCACTAGACATTGTTCCCATAGAAAATGG 5 0.125 No Hit ACTTCTTGCAGTGCAGGTCCTGTAGCTGAAGATATGTTTCACTGGCAAGC 5 0.125 No Hit GTTTTCTCAATTCGGTGAAGTTGTCTCTGTGAAAATACCAGTTGGAAAAG 5 0.125 No Hit GTTTGTCTAGCTTTCGCAGGAAATGGTGATGATGTTAAGGCAGCAATTTT 5 0.125 No Hit GATCAAAGGAGCGGAAGAATGAGATTAATCTTAGTGCTTGCTCTTGCGTT 5 0.125 No Hit TTCATATTCCATTTCTATTTTCTTGTCTTAGTTATTTTCTGATTATTTGG 5 0.125 No Hit TCTCAATCCAGTGGAAATGGTAGCCCAATAAAGAGAACCACTCTTCATGA 5 0.125 No Hit GTTTGTTATAGAAGCGATATCGGAGGTGTTTCCGGTGGATAAAAGAGGGG 5 0.125 No Hit AGCTCAACAAGAAGATCTACCGCATTGGCTCTGGCACTGACGATGCTAAC 5 0.125 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0 0.0 0.0 0.0 0.0 64-65 0.0 0.0 0.0 0.0 0.0 66-67 0.037500000000000006 0.0 0.0 0.0 0.0 68-69 0.05 0.0 0.0 0.0 0.0 70-71 0.0625 0.0 0.0 0.0 0.0 72-73 0.075 0.0 0.0 0.0 0.0 74-75 0.1 0.0 0.0 0.0 0.0 76-77 0.125 0.0 0.0 0.0 0.0 78-79 0.175 0.0 0.0 0.0 0.0 80-81 0.2 0.0 0.0 0.0 0.0 82-83 0.2 0.0 0.0 0.0 0.0 84-85 0.225 0.0 0.0 0.0 0.0 86-87 0.225 0.0 0.0 0.0 0.0 88-89 0.275 0.0 0.0 0.0 0.0 90-91 0.375 0.0 0.0 0.0 0.0 92-93 0.44999999999999996 0.0 0.0 0.0 0.0 94-95 0.5375 0.0 0.0 0.0 0.0 96-97 0.7124999999999999 0.0 0.0 0.0 0.0 98-99 0.9125 0.0 0.0 0.0 0.0 100-101 1.0875 0.0 0.0 0.0 0.0 102-103 1.325 0.0 0.0 0.0 0.0 104-105 1.55 0.0 0.0 0.0 0.0 106-107 1.725 0.0 0.0 0.0 0.0 108-109 1.8625 0.0 0.0 0.0 0.0 110-111 2.25 0.0 0.0 0.0 0.0 112-113 2.825 0.0 0.0 0.0 0.0 114-115 3.175 0.0 0.0 0.0 0.0 116-117 3.7 0.0 0.0 0.0 0.0 118-119 4.25 0.0 0.0 0.0 0.0 120-121 4.7375 0.0 0.0 0.0 0.0 122-123 5.25 0.0 0.0 0.0 0.0 124-125 6.012499999999999 0.0 0.0 0.0 0.0 126-127 7.0375 0.0 0.0 0.0 0.0 128-129 7.7625 0.0 0.0 0.0 0.0 130-131 8.4375 0.0 0.0 0.0 0.0 132-133 9.125 0.0 0.0 0.0 0.0 134-135 9.774999999999999 0.0 0.0 0.0 0.0 136-137 10.5375 0.0 0.0 0.0 0.0 138-139 11.3875 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content pass >>END_MODULE Read 1057794 spots for SRR26075367.sra Written 1057794 spots for SRR26075367.sra Read 1057794 spots for SRR26075367.sra Written 1057794 spots for SRR26075367.sra Read 1057794 spots for SRR26075367.sra Written 1057794 spots for SRR26075367.sra Read 1057794 spots for SRR26075367.sra Written 1057794 spots for SRR26075367.sra Read 1057794 spots for SRR26075367.sra Written 1057794 spots for SRR26075367.sra Read 1057794 spots for SRR26075367.sra Written 1057794 spots for SRR26075367.sra Read 1057794 spots for SRR26075367.sra Written 1057794 spots for SRR26075367.sra Read 1057794 spots for SRR26075367.sra Written 1057794 spots for SRR26075367.sra Read 1057794 spots for SRR26075367.sra Written 1057794 spots for SRR26075367.sra Read 1057794 spots for SRR26075367.sra Written 1057794 spots for SRR26075367.sra Read 1057794 spots for SRR26075367.sra Written 1057794 spots for SRR26075367.sra Read 1057794 spots for SRR26075367.sra Written 1057794 spots for SRR26075367.sra Read 1057794 spots for SRR26075367.sra Written 1057794 spots for SRR26075367.sra Read 1057794 spots for SRR26075367.sra Written 1057794 spots for SRR26075367.sra Read 1057794 spots for SRR26075367.sra Written 1057794 spots for SRR26075367.sra Read 1057794 spots for SRR26075367.sra Written 1057794 spots for SRR26075367.sra Read 1057807 spots for SRR26075367.sra Written 1057807 spots for SRR26075367.sra Read 1057794 spots for SRR26075367.sra Written 1057794 spots for SRR26075367.sra Read 1057794 spots for SRR26075367.sra Written 1057794 spots for SRR26075367.sra Read 1057794 spots for SRR26075367.sra Written 1057794 spots for SRR26075367.sra SRR ids: ['SRR26075367.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_sne2d0ew SRR26075367.sra spots: 21155893 blocks: [[1, 1057794], [1057795, 2115588], [2115589, 3173382], [3173383, 4231176], [4231177, 5288970], [5288971, 6346764], [6346765, 7404558], [7404559, 8462352], [8462353, 9520146], [9520147, 10577940], [10577941, 11635734], [11635735, 12693528], [12693529, 13751322], [13751323, 14809116], [14809117, 15866910], [15866911, 16924704], [16924705, 17982498], [17982499, 19040292], [19040293, 20098086], [20098087, 21155893]] SRR26075367 file size 7808267 SRR26075367 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o SRR26075367 SRR26075367_1.fastq SRR26075367_2.fastq Input file: SRR26075367_1.fastq Paired file: SRR26075367_2.fastq trimmed: SRR26075367-trimmed-pair1.fastq, SRR26075367-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Tue Feb 11 22:39:15 2025 >> started Tue Feb 11 22:39:40 2025 >> done (25.211s) 21155893 read pairs processed; of these: 138 ( 0.00%) short read pairs filtered out after trimming by size control 92039 ( 0.44%) empty read pairs filtered out after trimming by size control 21063716 (99.56%) read pairs available; of these: 3515174 (16.69%) trimmed read pairs available after processing 17548542 (83.31%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 18 15 0.00% 19 13 0.00% 20 14 0.00% 21 16 0.00% 22 22 0.00% 23 7 0.00% 24 18 0.00% 25 19 0.00% 26 20 0.00% 27 19 0.00% 28 25 0.00% 29 18 0.00% 30 28 0.00% 31 37 0.00% 32 22 0.00% 33 28 0.00% 34 35 0.00% 35 29 0.00% 36 24 0.00% 37 43 0.00% 38 40 0.00% 39 47 0.00% 40 62 0.00% 41 58 0.00% 42 54 0.00% 43 54 0.00% 44 76 0.00% 45 66 0.00% 46 60 0.00% 47 63 0.00% 48 84 0.00% 49 128 0.00% 50 92 0.00% 51 95 0.00% 52 163 0.00% 53 132 0.00% 54 148 0.00% 55 179 0.00% 56 188 0.00% 57 201 0.00% 58 231 0.00% 59 288 0.00% 60 283 0.00% 61 346 0.00% 62 320 0.00% 63 442 0.00% 64 456 0.00% 65 539 0.00% 66 546 0.00% 67 648 0.00% 68 744 0.00% 69 818 0.00% 70 1056 0.01% 71 1188 0.01% 72 1254 0.01% 73 1637 0.01% 74 1731 0.01% 75 2012 0.01% 76 2320 0.01% 77 2662 0.01% 78 2856 0.01% 79 3425 0.02% 80 3734 0.02% 81 4258 0.02% 82 4921 0.02% 83 5365 0.03% 84 6446 0.03% 85 6769 0.03% 86 7520 0.04% 87 8169 0.04% 88 8909 0.04% 89 10167 0.05% 90 10869 0.05% 91 12005 0.06% 92 12713 0.06% 93 13946 0.07% 94 15778 0.07% 95 17219 0.08% 96 18056 0.09% 97 19806 0.09% 98 20895 0.10% 99 22419 0.11% 100 23378 0.11% 101 24370 0.12% 102 26756 0.13% 103 28555 0.14% 104 30307 0.14% 105 31847 0.15% 106 33761 0.16% 107 35432 0.17% 108 37266 0.18% 109 38561 0.18% 110 39012 0.19% 111 41721 0.20% 112 43020 0.20% 113 44238 0.21% 114 46782 0.22% 115 47838 0.23% 116 49652 0.24% 117 52154 0.25% 118 53401 0.25% 119 55885 0.27% 120 55798 0.26% 121 58250 0.28% 122 58734 0.28% 123 61278 0.29% 124 63222 0.30% 125 64812 0.31% 126 67306 0.32% 127 68333 0.32% 128 69993 0.33% 129 71367 0.34% 130 73779 0.35% 131 73849 0.35% 132 75008 0.36% 133 76862 0.36% 134 78451 0.37% 135 81098 0.39% 136 81988 0.39% 137 84375 0.40% 138 84252 0.40% 139 86507 0.41% 140 88201 0.42% 141 89883 0.43% 142 90111 0.43% 143 90902 0.43% 144 95552 0.45% 145 94173 0.45% 146 95155 0.45% 147 96595 0.46% 148 97536 0.46% 149 98834 0.47% 150 100826 0.48% 151 17548542 83.31% 21063716 reads passed initial QC criterion=sequence-density sequence-density=0.22 sequence-density-rank=1 fanout-score=5.55 fanout-score-rank=23 prefix-density=0.32 prefix-fanout=3.8 sequence=TCCACACTTGCAGCCATTCTCAGCACC criterion=fanout-score sequence-density=0.04 sequence-density-rank=37 fanout-score=82.25 fanout-score-rank=1 prefix-density=0.34 prefix-fanout=9.6 sequence=CATCACCATCCCTGATTGATCTTTGATTCACAACAAGACCAACCAACCGTACAATGTTTACATAACTTGGGATAATCTCACAAGAAAGAAAGATCAATACAAAACTAGTCAGCTCAGGATGTTCCAGGCACGGTAGAACCGTAGAACCATAACAACAAGAGACATATTGCAGATGAGTACTGAAAAACAAAACACAGTACGTATTTACATGGGCAACCTTGGTTGAAGGCAACCTCATCAACGATGCTCGCTCTTCGTCTCTCCACTGTACATCCAGTCATAGACAGTGGGTGTGTTAGGCTGTGGCTTGTCAAAAACATGAGCACCAATATTCTTAGTAGCAAGGTTGCTACCAGGGTGGAACACGCTCCTCCAAACATTGCTACGCGCCGACACTGGGGTTGTAGGGGTCACTGGTGTCGTCGGTGTCCCTGGAGTTCCTGGCATAGTCATGGACCTCTGAAACTTATTAACAGGACTGCTCCCCTCTCCGACGTCAATATCTTTGATG criterion=sequence-density sequence-density=0.39 sequence-density-rank=1 fanout-score=2.63 fanout-score-rank=33 prefix-density=0.39 prefix-fanout=2.6 sequence=ATGTACCCTGACTTAGGTTTCTCAGA criterion=fanout-score sequence-density=0.01 sequence-density-rank=41 fanout-score=153.74 fanout-score-rank=1 prefix-density=0.10 prefix-fanout=8.3 sequence=GAGAAAAGATAAGCTAGGCAAGATGGTTTTACTAGACAAGATGTGGGATGATGTTGTTGCTGGACCTCAGCCAGAACGTGGCCTTGGCAAGCTTAGAAAGATCAGCACCAGACCACTTAACATCAAAGATATTGACGTCGGAGAGGGGAGCAGTCCTGTTAATAAGTTTCAGAGGTCCATGACTATGCCAGGAACTCCAGGGACACCGACGACACCAGTGACCCCTACAACCCCAGTGTCGGCGCGTAGCAATGTTTGGAGGAGCGTGTTCCACCCTGGTAGCAACCTTGCTACTAAGAATATTGGTGCTCATGTTTTTGACAAGCCACAGCCTAACACACCCACTGTCTATGACTGGATGTACAGTGGAGAGACGAAGAGCGAGCATCGTTGATGAGGTTGCCTTCAACCAAGGTTGCCCATGTAAATACGTACTGTGTTTTGTTTTTCAGTACTCATCTGCAATATGTCTCTTGTTGTTATGGTTCTACGGTTCTACCGTGCCTGGAAC SRR26075367 testing PE reads STAR mapping to Ensembl genome Started job on | Feb 11 22:40:21 Started mapping on | Feb 11 22:40:22 Finished on | Feb 11 22:43:30 Mapping speed, Million of reads per hour | 403.35 Number of input reads | 21063716 Average input read length | 294 UNIQUE READS: Uniquely mapped reads number | 19016456 Uniquely mapped reads % | 90.28% Average mapped length | 292.56 Number of splices: Total | 17359480 Number of splices: Annotated (sjdb) | 16893218 Number of splices: GT/AG | 17030513 Number of splices: GC/AG | 249716 Number of splices: AT/AC | 21336 Number of splices: Non-canonical | 57915 Mismatch rate per base, % | 0.40% Deletion rate per base | 0.04% Deletion average length | 3.28 Insertion rate per base | 0.02% Insertion average length | 2.50 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 535976 % of reads mapped to multiple loci | 2.54% Number of reads mapped to too many loci | 104377 % of reads mapped to too many loci | 0.50% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 6.29% % of reads unmapped: other | 0.39% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 1511284 1511284 1511284 N_multimapping 535976 535976 535976 N_noFeature 551736 18795201 680470 N_ambiguous 199033 1288 105726 UnstrandedReadsAssigned:18265687 PositiveStrandReadsAssigned:219967 NegativeStrandReadsAssigned:18230260 Dataset is classified negative stranded MeadianReadLen=151 20thPercentileLength=151 echo kmer=147 SRR26075367 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,400 [index] number of k-mers: 62,057,036 [index] number of equivalence classes: 130,681 [quant] running in paired-end mode [quant] will process pair 1: SRR26075367-trimmed-pair1.fastq SRR26075367-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 21,063,716 reads, 18,504,114 reads pseudoaligned [quant] estimated average fragment length: 208.767 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,145 rounds 52401 SRR26075367.ke.tsv 34699 SRR26075367.se.tsv 87100 total ==> SRR26075367.ke.tsv <== target_id length eff_length est_counts tpm Potri.005G200100.1.v4.1 2018 1810.23 1980 49.7901 Potri.005G024800.1.v4.1 1035 827.233 2236 123.043 Potri.004G059700.1.v4.1 961 753.233 0 0 Potri.007G009000.2.v4.1 1416 1208.23 0 0 Potri.003G141000.2.v4.1 2943 2735.23 841.433 14.0035 Potri.016G087400.1.v4.1 270 90.7844 1822 913.585 Potri.015G069301.1.v4.1 564 357.571 0 0 Potri.010G195200.1.v4.1 1773 1565.23 348 10.1207 Potri.012G127500.1.v4.1 977 769.233 16107 953.167 ==> SRR26075367.se.tsv <== Potri.001G166300.v4.1 0 Potri.001G448400.v4.1 33 Potri.001G233950.v4.1 0 Potri.001G122700.v4.1 359 Potri.001G212900.v4.1 0 Potri.001G182400.v4.1 0 Potri.001G256600.v4.1 0 Potri.001G040500.v4.1 3 Potri.001G416900.v4.1 0 Potri.001G452600.v4.1 1413 SRR26075367 completed mapping pipeline successfully