Starting /dee2/code/volunteer_pipeline.sh SRR26075368 current disk space = 3052376788992 free memory = 1490076056 SRR26075368 SRAfilesize 8dfb7878e17cbb1652fad703dcaeac34 SRR26075368.sra SRR26075368.sra file validated SRR26075368 is paired end SRR26075368 is conventional basespace SRR26075368 read1 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR26075368_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.582 37.0 37.0 37.0 37.0 37.0 2 36.6185 37.0 37.0 37.0 37.0 37.0 3 36.533 37.0 37.0 37.0 37.0 37.0 4 36.691 37.0 37.0 37.0 37.0 37.0 5 36.632 37.0 37.0 37.0 37.0 37.0 6 36.632 37.0 37.0 37.0 37.0 37.0 7 36.6335 37.0 37.0 37.0 37.0 37.0 8 36.6395 37.0 37.0 37.0 37.0 37.0 9 36.6395 37.0 37.0 37.0 37.0 37.0 10-14 36.6517 37.0 37.0 37.0 37.0 37.0 15-19 36.6122 37.0 37.0 37.0 37.0 37.0 20-24 36.573899999999995 37.0 37.0 37.0 37.0 37.0 25-29 36.4589 37.0 37.0 37.0 37.0 37.0 30-34 36.4015 37.0 37.0 37.0 37.0 37.0 35-39 36.3429 37.0 37.0 37.0 37.0 37.0 40-44 36.3394 37.0 37.0 37.0 37.0 37.0 45-49 36.2669 37.0 37.0 37.0 37.0 37.0 50-54 36.247699999999995 37.0 37.0 37.0 37.0 37.0 55-59 36.2101 37.0 37.0 37.0 37.0 37.0 60-64 36.2124 37.0 37.0 37.0 37.0 37.0 65-69 36.0612 37.0 37.0 37.0 37.0 37.0 70-74 36.0751 37.0 37.0 37.0 37.0 37.0 75-79 36.0447 37.0 37.0 37.0 37.0 37.0 80-84 35.924099999999996 37.0 37.0 37.0 37.0 37.0 85-89 35.9222 37.0 37.0 37.0 37.0 37.0 90-94 35.876 37.0 37.0 37.0 37.0 37.0 95-99 35.8693 37.0 37.0 37.0 37.0 37.0 100-104 35.8154 37.0 37.0 37.0 37.0 37.0 105-109 35.770399999999995 37.0 37.0 37.0 37.0 37.0 110-114 35.6799 37.0 37.0 37.0 37.0 37.0 115-119 35.57790000000001 37.0 37.0 37.0 37.0 37.0 120-124 35.6398 37.0 37.0 37.0 37.0 37.0 125-129 35.4379 37.0 37.0 37.0 37.0 37.0 130-134 35.3481 37.0 37.0 37.0 34.6 37.0 135-139 35.1903 37.0 37.0 37.0 29.8 37.0 140-144 35.0629 37.0 37.0 37.0 27.4 37.0 145-149 35.028099999999995 37.0 37.0 37.0 27.4 37.0 150-151 35.105000000000004 37.0 37.0 37.0 25.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.0 1101 2 0.0 1101 3 0.0 1101 4 0.0 1101 5 0.0 1101 6 0.0 1101 7 0.0 1101 8 0.0 1101 9 0.0 1101 10-14 0.0 1101 15-19 0.0 1101 20-24 0.0 1101 25-29 0.0 1101 30-34 0.0 1101 35-39 0.0 1101 40-44 0.0 1101 45-49 0.0 1101 50-54 0.0 1101 55-59 0.0 1101 60-64 0.0 1101 65-69 0.0 1101 70-74 0.0 1101 75-79 0.0 1101 80-84 0.0 1101 85-89 0.0 1101 90-94 0.0 1101 95-99 0.0 1101 100-104 0.0 1101 105-109 0.0 1101 110-114 0.0 1101 115-119 0.0 1101 120-124 0.0 1101 125-129 0.0 1101 130-134 0.0 1101 135-139 0.0 1101 140-144 0.0 1101 145-149 0.0 1101 150-151 0.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 15 2.0 16 0.0 17 0.0 18 1.0 19 0.0 20 1.0 21 2.0 22 4.0 23 7.0 24 4.0 25 8.0 26 5.0 27 12.0 28 22.0 29 20.0 30 28.0 31 39.0 32 58.0 33 85.0 34 158.0 35 453.0 36 2902.0 37 189.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 45.59118236472946 13.902805611222444 6.913827655310621 33.59218436873748 2 19.25 14.224999999999998 33.525 33.0 3 17.549999999999997 19.5 29.525000000000002 33.425 4 21.65 24.125 27.075 27.150000000000002 5 24.175 29.65 24.075 22.1 6 21.425 33.800000000000004 23.075000000000003 21.7 7 14.075 28.825 40.949999999999996 16.150000000000002 8 16.875 26.825 32.775 23.525 9 17.9 25.474999999999998 32.95 23.674999999999997 10-14 20.54 30.17 26.07 23.22 15-19 20.41 28.435 27.555000000000003 23.599999999999998 20-24 19.975 28.57 27.27 24.185000000000002 25-29 19.545 27.389999999999997 28.560000000000002 24.505 30-34 19.49 28.67 27.644999999999996 24.195 35-39 19.985 29.020000000000003 27.034999999999997 23.96 40-44 20.205000000000002 27.855 27.245 24.695 45-49 19.885 27.565 28.360000000000003 24.19 50-54 20.32 28.205000000000002 27.650000000000002 23.825 55-59 20.29 27.839999999999996 27.694999999999997 24.175 60-64 20.955 28.37 27.525 23.150000000000002 65-69 20.544999999999998 27.455000000000002 27.560000000000002 24.44 70-74 20.445 27.495000000000005 28.410000000000004 23.65 75-79 20.07 27.994999999999997 27.685 24.25 80-84 19.555 28.015 28.09 24.34 85-89 20.09 27.134999999999998 28.389999999999997 24.385 90-94 20.835 27.41 27.295 24.46 95-99 20.380000000000003 27.11 28.465 24.044999999999998 100-104 20.53 28.025 27.22 24.224999999999998 105-109 20.244999999999997 27.73 27.18 24.845 110-114 20.76 27.555000000000003 27.54 24.145 115-119 21.015 26.775 28.349999999999998 23.86 120-124 20.9 27.82 27.235 24.044999999999998 125-129 21.740000000000002 27.315 26.915 24.03 130-134 21.240000000000002 27.345000000000002 26.99 24.425 135-139 21.15 27.63 26.88 24.34 140-144 22.085 27.11 26.265 24.54 145-149 22.040000000000003 27.55 27.305 23.105 150-151 22.425 27.1125 25.974999999999998 24.4875 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.5 11 1.0 12 0.5 13 0.0 14 0.0 15 0.5 16 1.0 17 0.5 18 0.0 19 0.5 20 1.5 21 1.5 22 0.5 23 1.0 24 5.5 25 6.5 26 5.5 27 3.5 28 5.5 29 12.0 30 13.0 31 20.5 32 33.0 33 33.5 34 43.0 35 50.0 36 75.5 37 110.5 38 115.5 39 138.5 40 194.0 41 224.5 42 235.0 43 229.5 44 246.0 45 289.0 46 257.5 47 240.0 48 250.5 49 221.0 50 187.0 51 151.0 52 126.0 53 117.0 54 98.5 55 68.0 56 49.0 57 40.0 58 27.0 59 20.0 60 13.5 61 6.0 62 5.0 63 5.0 64 2.5 65 3.5 66 3.5 67 2.0 68 3.5 69 3.0 70 1.0 71 0.5 72 0.0 73 0.0 74 0.0 75 0.0 76 0.0 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150-151 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 62.150000000000006 #Duplication Level Percentage of deduplicated Percentage of total 1 63.757039420756236 39.625 2 21.802091713596138 27.1 3 8.3668543845535 15.6 4 3.781174577634755 9.4 5 1.3274336283185841 4.125 6 0.4022526146419952 1.5 7 0.36202735317779566 1.575 8 0.12067578439259855 0.6 9 0.04022526146419952 0.22499999999999998 >10 0.04022526146419952 0.25 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GCTTGAAGCAAATTTTTGCAGAATTGATTTGTCAAGTACATGACCTCCAA 10 0.25 No Hit TTGGCTTGGCACTGATTAGAGCCACGGCTGCTGTAGCAGACGCTGCTCCT 9 0.22499999999999998 No Hit TGGGCTTATAGACCTTGTAAATTTTCATGACATCTCCTTCCAAACCATTA 8 0.2 No Hit GGCAGATCTGATAGAGAATACCCATGTAAGTCATCAGATCTGAGCTAATT 8 0.2 No Hit CCTATACTAAAGTGAGTTTTCTTCTTTCTTTACACTACCATGCATTTTCA 8 0.2 No Hit CATATACTCAGGGGTCTCATCGGTGGCATAACCAAACATATGGCCCTGGT 7 0.17500000000000002 No Hit TTTCCAAATAAGCCTGTGATGCCACCTTTGCTGCCGCCATTCCTCCTCCA 7 0.17500000000000002 No Hit GCCTGTAGCAAGCAATAACCAAGAAAAACTGCTAGTCTGCAATCATTGCC 7 0.17500000000000002 No Hit GCATCCAAGAGCATCAAGCAAAATCCTCTGTTCTCCCAATCTTGAAAGTT 7 0.17500000000000002 No Hit TCCTGAATCAGCTGGAGGCAAGCTTGTTATCACCTTGAAGCTATAACCTT 7 0.17500000000000002 No Hit GCGACCAAACCCATAGCCACCACCATCTAGTAACAGAACCATATCCTGCA 7 0.17500000000000002 No Hit CAGCATCAAGAGGTCCGACAGAAGGTTTTGGAATTGGATCACGAGGAGTA 7 0.17500000000000002 No Hit ATACATCTCTCAGGGCATGAACAGTTCAGTAAATAGAAGGAAACTAGTGC 7 0.17500000000000002 No Hit CAGGAATTAAATTTGTTGAGTCTGAAACCATTGCAGAAGACCGTGATGGA 7 0.17500000000000002 No Hit CCTTGGCTTCGGACAAACCACAGACTCCACCACCACCATCATCCTCTCCC 6 0.15 No Hit CTCAAAAATTTCTGCATCGATGCCCATGACTCCTTTCCGGCCTTCCTTTG 6 0.15 No Hit CTGCCTTCTTCTGACGAGCTATGCGTCTGCGGGTCTTTCGGGCTGGTTGG 6 0.15 No Hit GGAGTAAGAGCAACTGGAGCAGTATTAGCTTGCAGATATCCTCTTTCCTA 6 0.15 No Hit ACTGCCACACTCTCCCGATTCTTTGACTGCTCATCGTGCAAAAACGTGCA 6 0.15 No Hit CCGTTTAATTGGTCCCTGACATACTCCTCCTGGCTGTTACCTGCAGTAAT 6 0.15 No Hit CTCCGATTCTCGACAAGTCAACTCAGCTGCATCATCATCATGATCATCCC 6 0.15 No Hit ACTCTCATCAGGCATGTGGTGCAACACGTACGGAAAATTCACAACCACAG 6 0.15 No Hit GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTAACGACATCTCGTAT 6 0.15 TruSeq Adapter, Index 19 (97% over 37bp) GTTATATCAAAGAGACTAGATCTTCGTCGTCGGCGATTCTGGTTATTTCT 6 0.15 No Hit GCCTAAGTATGTAAATCTTTGTCATCTCTGCCTGAAGGGTTCTAAGTGCC 5 0.125 No Hit ACAAAACAAAACTCTGTACAACAAGAACCCATAAATGGATCATCGTCCTA 5 0.125 No Hit TTTTGGTGTAAGCTGCCAGACGGGCCTATAGACCTTGTAAATTTTCATGA 5 0.125 No Hit CGCCGCTAACAGGGAGCAGACTAGATGCTATTTAGTGCGACGGAGGAGAG 5 0.125 No Hit AGCACTTCCAATGCCCTTGCAGCATCTTGAGGATTGCTGTAGTTAACATA 5 0.125 No Hit CAGAAGTTATTGCATTTGTAGCATTGTTTGGGCCACCCATGCCACTCACC 5 0.125 No Hit GCCATTACTCACGCTATGATCACACGGTCATGATTCCTCAACCAGACTTA 5 0.125 No Hit GTCCCAACTAGATGCCAATTCCCTGTCAACTCCATTAATTACATCCAACC 5 0.125 No Hit AGCAGATGGGAACTGGTCTGCTTGTTCTTCTGAGAGCTTGAACCCGTCCA 5 0.125 No Hit GTCTCTTCCTTAAAAGAAACAGATTGTGTTATTTTCACCTCCCCTGTCGT 5 0.125 No Hit CTCAAATCTGTATGACCAGAATATGCGATCCTAAGAGTACAGGGCCCAAG 5 0.125 No Hit GGTAAGGCCATGCATGCATAGTCTATCTTAGGACAACTACCTAGTCTTAT 5 0.125 No Hit GGCCTCCTCTCAGATAGCTCCATTCCTCTCCACGATACCAAGGAAGTTTC 5 0.125 No Hit GGCATTATAAGCAAACGATGAGGCTTCGAGGTGATCGAACGGAGGCCCTG 5 0.125 No Hit GTAAAAAAGAAGACTATCGTACAAAATGGATTAATTTATCGAAGGCATTG 5 0.125 No Hit TTTTTTTTTTCTAAAAATAATAAAATTATTATAGAATCTAGTATTTTCTA 5 0.125 No Hit AGCATGTATGGGATGGGATGGGATGTGATGCGATGTTGCTACCGTATCGG 5 0.125 No Hit GTCTCCAATCTCTGCAAAGAGCTCCCTTATGTCTTCATTGGTCACGCCAT 5 0.125 No Hit GTTGATTTCCTTTGCTCTGATTGTGCATTCTCTTCTGCGCGAAGTCGAGC 5 0.125 No Hit CACAAAATTATCGATGATTTCGGATAGATACATTTAAAAGTACACTTTCG 5 0.125 No Hit GTCTGCAACTTCATGGGGAAGCTCTTTCTCTATAGAGATGTTGTCAAGAT 5 0.125 No Hit GTAGCCATTTGTATTCATCACCACCTCAGGCGCCATCCAGTAAGGACTTC 5 0.125 No Hit GTCGGCGGAACCCTTTGCATTCACCACCGGGAAAACCTTCAGTCTTGCAC 5 0.125 No Hit GCCGTCATATCTCACCCCCTCCTTCTCTCCTCCTATCTATTATTACTAGT 5 0.125 No Hit ACACAATCGATTATAAATTAGTTATTACGACGAAACGGGATTGATGACGA 5 0.125 No Hit GTGGAAACATGCTAACAAAACCACACTGCACATCGCACGACAGTCATGGA 5 0.125 No Hit CGTCGCAAATGCATTTTAGTCCCGACCAACTAAATAAGTTGGTTTTATTT 5 0.125 No Hit GTCCCGGAACCATGATCATCATCAGAACTTGATCTTGCACTTGTTGTTAC 5 0.125 No Hit ACACGGAGGAGAGCGAGCTCTGCTTTGAGCTCCTTCAGCTGGGCCAGCAG 5 0.125 No Hit CCTAAAACAATCCTCGTTGTTCAATATACTCAGACAAAAATACATGGAAC 5 0.125 No Hit GTGATAGATTAGTGTAACTCAGCCTCAAAGCAGGTAAGATCTCGCTTGCT 5 0.125 No Hit CGATAAAGATCCAAACCCTACAATCCAACTCTAACGAGTATTTTCCTGTC 5 0.125 No Hit GCTCAATTAGTAAGAGGTGTGAAATAAACAAGATCATCTTGTAAAACTTG 5 0.125 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0 0.0 0.0 0.0 0.0 64-65 0.0 0.0 0.0 0.0 0.0 66-67 0.0 0.0 0.0 0.0 0.0 68-69 0.0125 0.0 0.0 0.0 0.0 70-71 0.025 0.0 0.0 0.0 0.0 72-73 0.075 0.0 0.0 0.0 0.0 74-75 0.0875 0.0 0.0 0.0 0.0 76-77 0.15 0.0 0.0 0.0 0.0 78-79 0.2 0.0 0.0 0.0 0.0 80-81 0.275 0.0 0.0 0.0 0.0 82-83 0.3 0.0 0.0 0.0 0.0 84-85 0.4 0.0 0.0 0.0 0.0 86-87 0.44999999999999996 0.0 0.0 0.0 0.0 88-89 0.5875 0.0 0.0 0.0 0.0 90-91 0.8375 0.0 0.0 0.0 0.0 92-93 0.925 0.0 0.0 0.0 0.0 94-95 0.9625 0.0 0.0 0.0 0.0 96-97 1.2 0.0 0.0 0.0 0.0 98-99 1.375 0.0 0.0 0.0 0.0 100-101 1.8 0.0 0.0 0.0 0.0 102-103 2.1125 0.0 0.0 0.0 0.0 104-105 2.35 0.0 0.0 0.0 0.0 106-107 2.65 0.0 0.0 0.0 0.0 108-109 2.9749999999999996 0.0 0.0 0.0 0.0 110-111 3.175 0.0 0.0 0.0 0.0 112-113 3.7375 0.0 0.0 0.0 0.0 114-115 4.1875 0.0 0.0 0.0 0.0 116-117 4.525 0.0 0.0 0.0 0.0 118-119 5.0 0.0 0.0 0.0 0.0 120-121 5.762499999999999 0.0 0.0 0.0 0.0 122-123 6.225 0.0 0.0 0.0 0.0 124-125 6.6625 0.0 0.0 0.0 0.0 126-127 7.0875 0.0 0.0 0.0 0.0 128-129 7.6625 0.0 0.0 0.0 0.0 130-131 8.15 0.0 0.0 0.0 0.0 132-133 8.55 0.0 0.0 0.0 0.0 134-135 8.925 0.0 0.0 0.0 0.0 136-137 9.525 0.0 0.0 0.0 0.0 138-139 9.95 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AATCTGT 10 0.006830828 145.0 5 GGGTAAT 10 0.006830828 145.0 1 TCAAAAC 10 0.006830828 145.0 8 CAGGGGG 10 0.006830828 145.0 9 TTTGGTC 10 0.006830828 145.0 8 TTGGTCC 10 0.006830828 145.0 9 CAGGATG 10 0.006830828 145.0 7 TTTAAAT 10 0.006830828 145.0 145 TCAGGGG 20 3.5877043E-4 108.75 8 TTCCTTC 30 0.0017973486 72.5 3 >>END_MODULE SRR26075368 read2 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR26075368_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.41025 37.0 37.0 37.0 37.0 37.0 2 36.312 37.0 37.0 37.0 37.0 37.0 3 36.407 37.0 37.0 37.0 37.0 37.0 4 36.2965 37.0 37.0 37.0 37.0 37.0 5 36.2735 37.0 37.0 37.0 37.0 37.0 6 36.2475 37.0 37.0 37.0 37.0 37.0 7 36.3455 37.0 37.0 37.0 37.0 37.0 8 36.335 37.0 37.0 37.0 37.0 37.0 9 36.4035 37.0 37.0 37.0 37.0 37.0 10-14 36.3154 37.0 37.0 37.0 37.0 37.0 15-19 36.29260000000001 37.0 37.0 37.0 37.0 37.0 20-24 36.2697 37.0 37.0 37.0 37.0 37.0 25-29 36.1502 37.0 37.0 37.0 37.0 37.0 30-34 36.0434 37.0 37.0 37.0 37.0 37.0 35-39 36.0138 37.0 37.0 37.0 37.0 37.0 40-44 35.9314 37.0 37.0 37.0 37.0 37.0 45-49 35.7962 37.0 37.0 37.0 37.0 37.0 50-54 35.7262 37.0 37.0 37.0 37.0 37.0 55-59 35.744400000000006 37.0 37.0 37.0 37.0 37.0 60-64 35.7913 37.0 37.0 37.0 37.0 37.0 65-69 35.651700000000005 37.0 37.0 37.0 37.0 37.0 70-74 35.521699999999996 37.0 37.0 37.0 37.0 37.0 75-79 35.473699999999994 37.0 37.0 37.0 37.0 37.0 80-84 35.5448 37.0 37.0 37.0 37.0 37.0 85-89 35.5437 37.0 37.0 37.0 37.0 37.0 90-94 35.4561 37.0 37.0 37.0 37.0 37.0 95-99 35.4485 37.0 37.0 37.0 37.0 37.0 100-104 35.3746 37.0 37.0 37.0 37.0 37.0 105-109 35.3523 37.0 37.0 37.0 37.0 37.0 110-114 35.2933 37.0 37.0 37.0 37.0 37.0 115-119 35.2103 37.0 37.0 37.0 34.6 37.0 120-124 35.17085 37.0 37.0 37.0 34.6 37.0 125-129 35.1179 37.0 37.0 37.0 27.4 37.0 130-134 35.094899999999996 37.0 37.0 37.0 27.4 37.0 135-139 34.91255 37.0 37.0 37.0 25.0 37.0 140-144 34.980599999999995 37.0 37.0 37.0 25.0 37.0 145-149 34.93795 37.0 37.0 37.0 25.0 37.0 150-151 34.7355 37.0 37.0 37.0 25.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.0 1101 2 0.0 1101 3 0.0 1101 4 0.0 1101 5 0.0 1101 6 0.0 1101 7 0.0 1101 8 0.0 1101 9 0.0 1101 10-14 0.0 1101 15-19 0.0 1101 20-24 0.0 1101 25-29 0.0 1101 30-34 0.0 1101 35-39 0.0 1101 40-44 0.0 1101 45-49 0.0 1101 50-54 0.0 1101 55-59 0.0 1101 60-64 0.0 1101 65-69 0.0 1101 70-74 0.0 1101 75-79 0.0 1101 80-84 0.0 1101 85-89 0.0 1101 90-94 0.0 1101 95-99 0.0 1101 100-104 0.0 1101 105-109 0.0 1101 110-114 0.0 1101 115-119 0.0 1101 120-124 0.0 1101 125-129 0.0 1101 130-134 0.0 1101 135-139 0.0 1101 140-144 0.0 1101 145-149 0.0 1101 150-151 0.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 12 1.0 13 6.0 14 10.0 15 8.0 16 8.0 17 7.0 18 4.0 19 3.0 20 8.0 21 6.0 22 12.0 23 16.0 24 16.0 25 20.0 26 8.0 27 12.0 28 17.0 29 19.0 30 16.0 31 38.0 32 44.0 33 70.0 34 127.0 35 579.0 36 2714.0 37 231.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 48.36209052263066 23.355838959739934 8.27706926731683 20.005001250312578 2 29.175 25.25 27.975 17.599999999999998 3 22.775000000000002 27.1 33.275 16.85 4 28.025 31.05 22.35 18.575 5 26.924999999999997 35.025 19.925 18.125 6 21.3 38.675 22.45 17.575 7 23.125 21.975 36.05 18.85 8 23.0 25.324999999999996 26.55 25.124999999999996 9 23.974999999999998 25.124999999999996 27.675 23.225 10-14 26.619999999999997 28.46 24.7 20.22 15-19 24.82 27.655 26.51 21.015 20-24 25.405 27.85 26.115 20.630000000000003 25-29 25.495 28.18 25.990000000000002 20.335 30-34 24.39 28.605000000000004 26.505000000000003 20.5 35-39 25.405 28.21 26.205000000000002 20.18 40-44 25.345000000000002 29.175 25.31 20.169999999999998 45-49 25.82 27.529999999999998 26.31 20.34 50-54 23.925 27.994999999999997 27.04 21.04 55-59 25.005 28.28 26.775 19.939999999999998 60-64 25.785000000000004 27.67 26.650000000000002 19.895 65-69 24.759999999999998 29.15 25.979999999999997 20.11 70-74 25.15 28.325 26.135 20.39 75-79 24.905 28.665000000000003 26.435 19.994999999999997 80-84 24.32 28.235 27.04 20.405 85-89 25.319999999999997 27.88 26.695 20.105 90-94 24.19 28.515 26.745 20.549999999999997 95-99 25.045 28.349999999999998 26.695 19.91 100-104 24.93 28.4 26.46 20.21 105-109 24.59 28.105000000000004 27.195000000000004 20.11 110-114 25.71 27.975 26.135 20.18 115-119 25.295 28.54 26.450000000000003 19.715 120-124 25.911295564778236 28.286414320716034 26.08630431521576 19.715985799289964 125-129 26.345000000000002 28.435 25.735000000000003 19.485 130-134 25.91 28.999999999999996 25.869999999999997 19.220000000000002 135-139 24.77871680752113 28.399259888983348 26.944041606240937 19.877981697254587 140-144 26.300260052010405 28.180636127225444 26.580316063212646 18.93878775755151 145-149 27.526881720430108 28.637159289822456 25.95648912228057 17.879469867466867 150-151 26.84421105276319 28.89472368092023 25.143785946486624 19.117279319829958 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.5 7 0.5 8 0.5 9 0.5 10 1.5 11 3.0 12 1.5 13 1.5 14 1.5 15 0.0 16 1.0 17 1.5 18 1.5 19 2.0 20 2.0 21 3.5 22 4.0 23 2.0 24 1.5 25 2.0 26 2.5 27 3.5 28 3.5 29 8.5 30 13.5 31 12.5 32 18.0 33 29.0 34 38.5 35 48.5 36 67.0 37 85.5 38 114.0 39 139.0 40 164.0 41 215.5 42 253.5 43 252.0 44 277.0 45 299.5 46 284.5 47 257.0 48 224.0 49 198.5 50 163.5 51 131.5 52 122.5 53 113.0 54 85.0 55 66.5 56 54.5 57 34.0 58 19.0 59 16.5 60 13.5 61 16.0 62 17.0 63 13.0 64 8.5 65 3.5 66 3.0 67 3.5 68 1.0 69 1.0 70 1.5 71 1.0 72 1.0 73 1.0 74 1.0 75 0.5 76 0.5 77 2.5 78 2.5 79 1.5 80 2.0 81 1.5 82 1.0 83 1.5 84 2.5 85 1.5 86 0.5 87 1.0 88 1.5 89 1.5 90 1.5 91 3.0 92 3.5 93 2.0 94 0.5 95 0.0 96 0.0 97 1.0 98 2.5 99 3.0 100 15.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.025 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.005 125-129 0.0 130-134 0.0 135-139 0.015 140-144 0.02 145-149 0.025 150-151 0.025 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 62.525 #Duplication Level Percentage of deduplicated Percentage of total 1 65.77369052379048 41.125 2 20.151939224310276 25.2 3 8.236705317872852 15.45 4 3.5985605757696923 9.0 5 1.2395041983206718 3.875 6 0.3598560575769692 1.35 7 0.3198720511795282 1.4000000000000001 8 0.1599360255897641 0.8 9 0.03998400639744103 0.22499999999999998 >10 0.11995201919232307 1.575 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG 40 1.0 No Hit GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGT 13 0.325 No Hit GGAAAAGATGATGGATTATCGGCAACAACTCCCTTGAGGGGCTGGGAAGA 10 0.25 No Hit AGGAGGAGGAGGAGGAGGAGAAGAAGAGAGTAATTATCTCCAAAATTCAT 9 0.22499999999999998 No Hit ATGCAGTATCAAATGCTGATATACAAAGAGCAGCAGGTCTAATGCATGTC 8 0.2 No Hit TATTTTCTTTGAGAGTGCATAGATTTGTGTTGATATAGAAAACAATGGCA 8 0.2 No Hit GATATTGCCCAGGGTGTCCACGGTCACTTTACCAAGCGGCCAGAGGAGAT 8 0.2 No Hit GGTGAAATTGGGAGGTTTGATGGCGAGCAGTTTACACCAAGGTTTTCTGG 8 0.2 No Hit GGTCACTGTTGGTGAAGTTGTACGTGTTAATCCTTCCTTGAAGAGTATTG 7 0.17500000000000002 No Hit CAGGCCCCGCAGCAAGCTTTGCTTCGACCCCCTCCCAACTTGTCAATGCC 7 0.17500000000000002 No Hit GTCAGAAATACCGCGTGGCTTTAAGGATTGCTAAGGATCCACGTTTTGAG 7 0.17500000000000002 No Hit AGCAAGGCGAAAGCCATTTGGATGCAATGTTAAGAGGAGAAGTTTTGACT 7 0.17500000000000002 No Hit AAAAGATCAAAACTTTAGCACATTCAAGCAAAAAACTAAATAAAGTAGGA 7 0.17500000000000002 No Hit CTCCAATTTCCATCTGGGTGGAAAAGAATTATATCTGCAAATTGCAACCT 7 0.17500000000000002 No Hit TTTCTTGGATCTTGCCAAGTTGGTTTCTGCAGGCCATTTTCCTGGACGAG 7 0.17500000000000002 No Hit GGCCAAAGAAGATTCTGAGGCCTACTCTGCATGGGCTCCTGATCCAGTTA 7 0.17500000000000002 No Hit GTCTGGCTTATTTGAGGAGAAAGAGCAGAAGAAAGAAGTGAGGTTTACAT 6 0.15 No Hit GCCAAACAGAGGGAAGAGAAGTAGTTTGTTAGTGATGATCATGATCATCG 6 0.15 No Hit GAGGCTTGCTGTGGACAGGTGGGGCCGTGTTGAAGTCACTGAACCTGCTG 6 0.15 No Hit ATGGGTTTCTTGGTTTGCCCGTTGAGTTTGAACCTGAGGTTCCCAGGAGA 6 0.15 No Hit ACCATGAAAGCACATTACGACTGTTAAAAGCAAAAAAAGTAAGAAGAAAT 6 0.15 No Hit GTGGGATTTGGAGCGGAGACACAGCGGTCTTATAAAGGGAGGCATTGTAA 6 0.15 No Hit CGCGAGCGCAAAAGAGGGGTTCCATGGACTGAGGAAGAGCACAAGCTTTT 6 0.15 No Hit CCTCTTTGTGGAGGGGTCTTCGTGGCAGCTCAGCTCAGAGGTTTGGCTTG 6 0.15 No Hit GCAATGTGCCATTTGAGTTCCATGATGTTGCCATGGATGGTTGTGAGGTT 6 0.15 No Hit GAATGCCAGTGAGGTCTCAGAGCCACAAGTAATTGAACCATCTGAAGTGA 5 0.125 No Hit GCTAGGGTTTTCTGAAAGGAACACAACGGAGCAGCGGCAGAGCCTTACAA 5 0.125 No Hit GTATGCGCTCATGATTCGTGTAGACCTGCGATTACTTTTGCAGTGGAATT 5 0.125 No Hit GCCACAGAATGTGAATGGAGGGGCTAATAACCCAAATTTTGTAACGACAT 5 0.125 No Hit GTTCTCGGCAGAGATTCAGCAGATCGAGAAGGAGATAGACAGAAGGAATG 5 0.125 No Hit CATAAACGGCCGCGCCTTTCTCTCTAACAGGTAAGACATGGTTAAGCATA 5 0.125 No Hit ACAACCTGTTACCCTTCAAGGTGAAGATTTGAAGCAATTTAACAGCGAAA 5 0.125 No Hit ACGGTCACGAGCTAGACATGGGTTTTGGATATAGATAGTGGATTTCGACT 5 0.125 No Hit GTGGATATGACAAGCTCACCTGAAACGTCATACTCAGTGGAAGACCATGT 5 0.125 No Hit GTGCTAGATACTAGTAGTAGTTGCAGACAGAGAGCTAGTGTTAGACAGGT 5 0.125 No Hit ATTCTTTCTGGGCTTGCCTATTTGCATGGAAGAAATACAGTTCACAGGGA 5 0.125 No Hit CCTAAAGACACAGACTTTGTTCTTCTTCTTTATTTACAAACCCCAAAAAA 5 0.125 No Hit GTAAATAGCATGGATCCTAGTGCACCTCTATCAGAGCAGACAATAGGTCA 5 0.125 No Hit ACAGCTTCCATGATGATTCCAAAAGGGAAAGAAATGCCAAAAGCCGATAC 5 0.125 No Hit GTCGAATTCCTCACTCCCATCTTCCATCTATTAATTCAACCTCCTGAAGT 5 0.125 No Hit GCTAGATACTAGTAGTAGTTGCAGACAGAGAGCTAGTGTTAGACAGGTTC 5 0.125 No Hit CTCTTCTTTTGTTCTTTTTGTAGATACCAATTGGGATTTCGGGAGCAACC 5 0.125 No Hit GTTTCAATCTGTCTTTTGAAAACTGCATCTGCTGTATAGTGATTTTGTCA 5 0.125 No Hit GGAGACTGCGGGCTCTATCAGGATTTGGCAGTACACAATCGGAGGGAAAG 5 0.125 No Hit GGACAGAGGAGGAGCACAGGAGATTCCTTTTTGGATTGCAGAAAGTGGGA 5 0.125 No Hit GTTGTTCCTTCGCAAGATGGACAGAGAATGAGGGCTTTGTGATTGCAAAT 5 0.125 No Hit CAACAACCACAAGTACAACAGTGAAGATCAAGATTACAAAGAAACAACTC 5 0.125 No Hit TATGCATAAGGTTTTTTATTTACATTTACTATTTTTTTATGTAAAAAAAC 5 0.125 No Hit TGAACCGAAGTGGTGGTTTTCATTAGAACGTATTCATGTCTAAATACCCG 5 0.125 No Hit AGTTATCAGTTAGGATATTGGTGGATCATTCGATAATTGAAAGTTTTGCT 5 0.125 No Hit AACCACATCTCTTGCCTCCTCCTCTCTTTCGTTCTCTCCCTCCCTCCCTG 5 0.125 No Hit ACTTCATAGATGGAGATTCTGAGCTTCACTCTGTGCCGAGAGTTTGTGAT 5 0.125 No Hit AGGATCTGTTCTATAGGATCGTACCTCTGTATCCTTTATCACTAAGGAGA 5 0.125 No Hit GAACGTTTCATACCAACATTGAAATTAGGATGGAATGAGGTTATATTCTT 5 0.125 No Hit GCTGGTATAGCCTGTATCATGAAGAGATTGCGTGGCAAGGAGGATGTCCA 5 0.125 No Hit TGAATTTGCGGATCTGAATCAAGCTATTGCGATGATATCGTATTATGCAT 5 0.125 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0 0.0 0.0 0.0 0.0 64-65 0.0 0.0 0.0 0.0 0.0 66-67 0.0 0.0 0.0 0.0 0.0 68-69 0.0125 0.0 0.0 0.0 0.0 70-71 0.025 0.0 0.0 0.0 0.0 72-73 0.075 0.0 0.0 0.0 0.0 74-75 0.0875 0.0 0.0 0.0 0.0 76-77 0.15 0.0 0.0 0.0 0.0 78-79 0.2 0.0 0.0 0.0 0.0 80-81 0.2875 0.0 0.0 0.0 0.0 82-83 0.325 0.0 0.0 0.0 0.0 84-85 0.44999999999999996 0.0 0.0 0.0 0.0 86-87 0.525 0.0 0.0 0.0 0.0 88-89 0.6625 0.0 0.0 0.0 0.0 90-91 0.9125 0.0 0.0 0.0 0.0 92-93 1.0 0.0 0.0 0.0 0.0 94-95 1.0375 0.0 0.0 0.0 0.0 96-97 1.275 0.0 0.0 0.0 0.0 98-99 1.475 0.0 0.0 0.0 0.0 100-101 1.9 0.0 0.0 0.0 0.0 102-103 2.2125000000000004 0.0 0.0 0.0 0.0 104-105 2.45 0.0 0.0 0.0 0.0 106-107 2.75 0.0 0.0 0.0 0.0 108-109 3.075 0.0 0.0 0.0 0.0 110-111 3.3 0.0 0.0 0.0 0.0 112-113 3.8625 0.0 0.0 0.0 0.0 114-115 4.3375 0.0 0.0 0.0 0.0 116-117 4.675 0.0 0.0 0.0 0.0 118-119 5.075 0.0 0.0 0.0 0.0 120-121 5.8375 0.0 0.0 0.0 0.0 122-123 6.300000000000001 0.0 0.0 0.0 0.0 124-125 6.7375 0.0 0.0 0.0 0.0 126-127 7.125 0.0 0.0 0.0 0.0 128-129 7.6625 0.0 0.0 0.0 0.0 130-131 8.125 0.0 0.0 0.0 0.0 132-133 8.525 0.0 0.0 0.0 0.0 134-135 8.9 0.0 0.0 0.0 0.0 136-137 9.5 0.0 0.0 0.0 0.0 138-139 9.9375 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTTTGG 10 0.006830828 145.0 145 GAAGATA 10 0.006830828 145.0 8 ATGTGGT 10 0.006830828 145.0 1 TTAAGGA 10 0.006830828 145.0 3 TGTGGTC 10 0.006830828 145.0 2 TCCAAGG 10 0.006830828 145.0 7 GTGGTCC 10 0.006830828 145.0 3 CCAAGGG 15 1.1411342E-4 145.0 8 TTTAAGG 10 0.006830828 145.0 2 TAAGGAA 10 0.006830828 145.0 4 CAAGGGA 25 8.7132835E-4 87.0 9 AAGGAAG 25 8.7132835E-4 87.0 5 >>END_MODULE Read 925246 spots for SRR26075368.sra Written 925246 spots for SRR26075368.sra Read 925246 spots for SRR26075368.sra Written 925246 spots for SRR26075368.sra Read 925246 spots for SRR26075368.sra Written 925246 spots for SRR26075368.sra Read 925246 spots for SRR26075368.sra Written 925246 spots for SRR26075368.sra Read 925246 spots for SRR26075368.sra Written 925246 spots for SRR26075368.sra Read 925246 spots for SRR26075368.sra Written 925246 spots for SRR26075368.sra Read 925246 spots for SRR26075368.sra Written 925246 spots for SRR26075368.sra Read 925246 spots for SRR26075368.sra Written 925246 spots for SRR26075368.sra Read 925246 spots for SRR26075368.sra Written 925246 spots for SRR26075368.sra Read 925246 spots for SRR26075368.sra Written 925246 spots for SRR26075368.sra Read 925246 spots for SRR26075368.sra Written 925246 spots for SRR26075368.sra Read 925246 spots for SRR26075368.sra Written 925246 spots for SRR26075368.sra Read 925246 spots for SRR26075368.sra Written 925246 spots for SRR26075368.sra Read 925246 spots for SRR26075368.sra Written 925246 spots for SRR26075368.sra Read 925246 spots for SRR26075368.sra Written 925246 spots for SRR26075368.sra Read 925246 spots for SRR26075368.sra Written 925246 spots for SRR26075368.sra Read 925246 spots for SRR26075368.sra Written 925246 spots for SRR26075368.sra Read 925246 spots for SRR26075368.sra Written 925246 spots for SRR26075368.sra Read 925246 spots for SRR26075368.sra Written 925246 spots for SRR26075368.sra Read 925265 spots for SRR26075368.sra Written 925265 spots for SRR26075368.sra SRR ids: ['SRR26075368.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_1ebbqvvc SRR26075368.sra spots: 18504939 blocks: [[1, 925246], [925247, 1850492], [1850493, 2775738], [2775739, 3700984], [3700985, 4626230], [4626231, 5551476], [5551477, 6476722], [6476723, 7401968], [7401969, 8327214], [8327215, 9252460], [9252461, 10177706], [10177707, 11102952], [11102953, 12028198], [12028199, 12953444], [12953445, 13878690], [13878691, 14803936], [14803937, 15729182], [15729183, 16654428], [16654429, 17579674], [17579675, 18504939]] SRR26075368 file size 6828479 SRR26075368 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o SRR26075368 SRR26075368_1.fastq SRR26075368_2.fastq Input file: SRR26075368_1.fastq Paired file: SRR26075368_2.fastq trimmed: SRR26075368-trimmed-pair1.fastq, SRR26075368-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Tue Feb 11 23:08:42 2025 >> started Tue Feb 11 23:09:02 2025 >> done (20.267s) 18504939 read pairs processed; of these: 88 ( 0.00%) short read pairs filtered out after trimming by size control 54306 ( 0.29%) empty read pairs filtered out after trimming by size control 18450545 (99.71%) read pairs available; of these: 2542630 (13.78%) trimmed read pairs available after processing 15907915 (86.22%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 18 3 0.00% 19 8 0.00% 20 10 0.00% 21 11 0.00% 22 10 0.00% 23 17 0.00% 24 15 0.00% 25 16 0.00% 26 15 0.00% 27 23 0.00% 28 20 0.00% 29 12 0.00% 30 26 0.00% 31 25 0.00% 32 23 0.00% 33 31 0.00% 34 30 0.00% 35 27 0.00% 36 20 0.00% 37 21 0.00% 38 35 0.00% 39 38 0.00% 40 67 0.00% 41 36 0.00% 42 44 0.00% 43 64 0.00% 44 59 0.00% 45 66 0.00% 46 82 0.00% 47 95 0.00% 48 69 0.00% 49 82 0.00% 50 101 0.00% 51 115 0.00% 52 114 0.00% 53 139 0.00% 54 151 0.00% 55 168 0.00% 56 170 0.00% 57 242 0.00% 58 238 0.00% 59 235 0.00% 60 295 0.00% 61 386 0.00% 62 414 0.00% 63 500 0.00% 64 551 0.00% 65 546 0.00% 66 675 0.00% 67 703 0.00% 68 787 0.00% 69 994 0.01% 70 1099 0.01% 71 1240 0.01% 72 1481 0.01% 73 1678 0.01% 74 1849 0.01% 75 2139 0.01% 76 2318 0.01% 77 2454 0.01% 78 2842 0.02% 79 3001 0.02% 80 3629 0.02% 81 3990 0.02% 82 4546 0.02% 83 5174 0.03% 84 5748 0.03% 85 6229 0.03% 86 6921 0.04% 87 7296 0.04% 88 7741 0.04% 89 8736 0.05% 90 9336 0.05% 91 9952 0.05% 92 11009 0.06% 93 12441 0.07% 94 13199 0.07% 95 14273 0.08% 96 15510 0.08% 97 15952 0.09% 98 16322 0.09% 99 17580 0.10% 100 18136 0.10% 101 19095 0.10% 102 20439 0.11% 103 21454 0.12% 104 22601 0.12% 105 24044 0.13% 106 25760 0.14% 107 26274 0.14% 108 27314 0.15% 109 27766 0.15% 110 27887 0.15% 111 29803 0.16% 112 30268 0.16% 113 32053 0.17% 114 33504 0.18% 115 35252 0.19% 116 36257 0.20% 117 37401 0.20% 118 38080 0.21% 119 38984 0.21% 120 39145 0.21% 121 39715 0.22% 122 41245 0.22% 123 42773 0.23% 124 44785 0.24% 125 45551 0.25% 126 47804 0.26% 127 48990 0.27% 128 48873 0.26% 129 50274 0.27% 130 51402 0.28% 131 51526 0.28% 132 52989 0.29% 133 53738 0.29% 134 55486 0.30% 135 56925 0.31% 136 58103 0.31% 137 58620 0.32% 138 60084 0.33% 139 61574 0.33% 140 61995 0.34% 141 62542 0.34% 142 63662 0.35% 143 65019 0.35% 144 65282 0.35% 145 67107 0.36% 146 68061 0.37% 147 69325 0.38% 148 70749 0.38% 149 70208 0.38% 150 72397 0.39% 151 15907915 86.22% 18450545 reads passed initial QC criterion=sequence-density sequence-density=0.21 sequence-density-rank=1 fanout-score=13.49 fanout-score-rank=8 prefix-density=0.16 prefix-fanout=13.5 sequence=GGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTAACGACATCTCGTATGCCGTCTTCTGCTTGAAA criterion=fanout-score sequence-density=0.02 sequence-density-rank=37 fanout-score=122.59 fanout-score-rank=1 prefix-density=0.17 prefix-fanout=12.2 sequence=TTCACCATTTTACTTCACCAATTCCTTAGAGATGTAATAGCCCATAACAATAGGAAATATCAGAAATCCAATAAGAATCAGCAATTCAGGAAGAAATATGACAAGGAGTAGTAGTGTGGATGTTGTTGTTAGACACTTCTTTTTGTCTTTAAATATAAGGCGTGGTAGAATTACTGGCACTCCAATGATTCCATATAACGGCCATAATGGAGCTATAGAATACAACACCAACGTCGCAAAAAACCAGCAAAAATTCTTAACATT criterion=sequence-density sequence-density=0.17 sequence-density-rank=1 fanout-score=2.98 fanout-score-rank=36 prefix-density=0.20 prefix-fanout=2.6 sequence=TACAACATCCAGAAGGAGTCCACCCTCCACTTGGTGCTTCG criterion=fanout-score sequence-density=0.10 sequence-density-rank=20 fanout-score=50.78 fanout-score-rank=1 prefix-density=0.37 prefix-fanout=13.5 sequence=AGAAAAGAAAGA SRR26075368 testing PE reads STAR mapping to Ensembl genome Started job on | Feb 11 23:09:45 Started mapping on | Feb 11 23:09:45 Finished on | Feb 11 23:12:47 Mapping speed, Million of reads per hour | 364.96 Number of input reads | 18450545 Average input read length | 295 UNIQUE READS: Uniquely mapped reads number | 16212810 Uniquely mapped reads % | 87.87% Average mapped length | 293.57 Number of splices: Total | 12882729 Number of splices: Annotated (sjdb) | 12569235 Number of splices: GT/AG | 12653416 Number of splices: GC/AG | 169488 Number of splices: AT/AC | 14092 Number of splices: Non-canonical | 45733 Mismatch rate per base, % | 0.40% Deletion rate per base | 0.04% Deletion average length | 3.42 Insertion rate per base | 0.02% Insertion average length | 2.59 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 548445 % of reads mapped to multiple loci | 2.97% Number of reads mapped to too many loci | 48064 % of reads mapped to too many loci | 0.26% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 8.24% % of reads unmapped: other | 0.66% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 1689290 1689290 1689290 N_multimapping 548445 548445 548445 N_noFeature 435637 16019155 560378 N_ambiguous 178098 1273 108499 UnstrandedReadsAssigned:15599075 PositiveStrandReadsAssigned:192382 NegativeStrandReadsAssigned:15543933 Dataset is classified negative stranded MeadianReadLen=151 20thPercentileLength=151 echo kmer=147 SRR26075368 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,400 [index] number of k-mers: 62,057,036 [index] number of equivalence classes: 130,681 [quant] running in paired-end mode [quant] will process pair 1: SRR26075368-trimmed-pair1.fastq SRR26075368-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 18,450,545 reads, 15,977,392 reads pseudoaligned [quant] estimated average fragment length: 219.32 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,326 rounds 52401 SRR26075368.ke.tsv 34699 SRR26075368.se.tsv 87100 total ==> SRR26075368.ke.tsv <== target_id length eff_length est_counts tpm Potri.005G200100.1.v4.1 2018 1799.68 3009 96.6744 Potri.005G024800.1.v4.1 1035 816.68 4675 330.99 Potri.004G059700.1.v4.1 961 742.686 10 0.778538 Potri.007G009000.2.v4.1 1416 1197.68 0 0 Potri.003G141000.2.v4.1 2943 2724.68 680 14.4304 Potri.016G087400.1.v4.1 270 87.4036 792.567 524.314 Potri.015G069301.1.v4.1 564 348.171 0 0 Potri.010G195200.1.v4.1 1773 1554.68 101.776 3.78519 Potri.012G127500.1.v4.1 977 758.686 6033 459.787 ==> SRR26075368.se.tsv <== Potri.001G166300.v4.1 0 Potri.001G448400.v4.1 160 Potri.001G233950.v4.1 0 Potri.001G122700.v4.1 125 Potri.001G212900.v4.1 0 Potri.001G182400.v4.1 0 Potri.001G256600.v4.1 0 Potri.001G040500.v4.1 0 Potri.001G416900.v4.1 0 Potri.001G452600.v4.1 314 SRR26075368 completed mapping pipeline successfully