Starting /dee2/code/volunteer_pipeline.sh SRR26075369 current disk space = 3052394713088 free memory = 1390710744 SRR26075369 SRAfilesize 2e4417c8a89e7c27995532963ca5f973 SRR26075369.sra SRR26075369.sra file validated SRR26075369 is paired end SRR26075369 is conventional basespace SRR26075369 read1 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR26075369_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.5855 37.0 37.0 37.0 37.0 37.0 2 36.615 37.0 37.0 37.0 37.0 37.0 3 36.637 37.0 37.0 37.0 37.0 37.0 4 36.7205 37.0 37.0 37.0 37.0 37.0 5 36.7425 37.0 37.0 37.0 37.0 37.0 6 36.6205 37.0 37.0 37.0 37.0 37.0 7 36.6575 37.0 37.0 37.0 37.0 37.0 8 36.6425 37.0 37.0 37.0 37.0 37.0 9 36.693 37.0 37.0 37.0 37.0 37.0 10-14 36.634499999999996 37.0 37.0 37.0 37.0 37.0 15-19 36.628400000000006 37.0 37.0 37.0 37.0 37.0 20-24 36.6005 37.0 37.0 37.0 37.0 37.0 25-29 36.4972 37.0 37.0 37.0 37.0 37.0 30-34 36.4587 37.0 37.0 37.0 37.0 37.0 35-39 36.3894 37.0 37.0 37.0 37.0 37.0 40-44 36.31160000000001 37.0 37.0 37.0 37.0 37.0 45-49 36.3294 37.0 37.0 37.0 37.0 37.0 50-54 36.263799999999996 37.0 37.0 37.0 37.0 37.0 55-59 36.3059 37.0 37.0 37.0 37.0 37.0 60-64 36.1884 37.0 37.0 37.0 37.0 37.0 65-69 36.1494 37.0 37.0 37.0 37.0 37.0 70-74 36.1429 37.0 37.0 37.0 37.0 37.0 75-79 36.02720000000001 37.0 37.0 37.0 37.0 37.0 80-84 36.05650000000001 37.0 37.0 37.0 37.0 37.0 85-89 35.899699999999996 37.0 37.0 37.0 37.0 37.0 90-94 35.8718 37.0 37.0 37.0 37.0 37.0 95-99 35.8119 37.0 37.0 37.0 37.0 37.0 100-104 35.846 37.0 37.0 37.0 37.0 37.0 105-109 35.7056 37.0 37.0 37.0 37.0 37.0 110-114 35.5545 37.0 37.0 37.0 37.0 37.0 115-119 35.474900000000005 37.0 37.0 37.0 37.0 37.0 120-124 35.4397 37.0 37.0 37.0 37.0 37.0 125-129 35.339999999999996 37.0 37.0 37.0 34.6 37.0 130-134 35.227999999999994 37.0 37.0 37.0 32.2 37.0 135-139 35.0117 37.0 37.0 37.0 29.8 37.0 140-144 34.9836 37.0 37.0 37.0 25.0 37.0 145-149 34.9069 37.0 37.0 37.0 25.0 37.0 150-151 34.670500000000004 37.0 37.0 37.0 25.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.0 1101 2 0.0 1101 3 0.0 1101 4 0.0 1101 5 0.0 1101 6 0.0 1101 7 0.0 1101 8 0.0 1101 9 0.0 1101 10-14 0.0 1101 15-19 0.0 1101 20-24 0.0 1101 25-29 0.0 1101 30-34 0.0 1101 35-39 0.0 1101 40-44 0.0 1101 45-49 0.0 1101 50-54 0.0 1101 55-59 0.0 1101 60-64 0.0 1101 65-69 0.0 1101 70-74 0.0 1101 75-79 0.0 1101 80-84 0.0 1101 85-89 0.0 1101 90-94 0.0 1101 95-99 0.0 1101 100-104 0.0 1101 105-109 0.0 1101 110-114 0.0 1101 115-119 0.0 1101 120-124 0.0 1101 125-129 0.0 1101 130-134 0.0 1101 135-139 0.0 1101 140-144 0.0 1101 145-149 0.0 1101 150-151 0.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 20 1.0 21 0.0 22 3.0 23 2.0 24 7.0 25 7.0 26 8.0 27 15.0 28 17.0 29 26.0 30 25.0 31 48.0 32 58.0 33 112.0 34 185.0 35 502.0 36 2813.0 37 171.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 34.856855851330984 13.485685585133098 9.141135107985937 42.51632345554997 2 17.724999999999998 15.275 36.125 30.875000000000004 3 18.275 16.175 27.525 38.025 4 21.65 23.75 24.275 30.325000000000003 5 24.45 29.375 24.425 21.75 6 21.7 34.325 21.025 22.95 7 14.099999999999998 27.025 41.575 17.299999999999997 8 17.4 29.025000000000002 31.175000000000004 22.400000000000002 9 17.775 24.025 33.225 24.975 10-14 19.35387077415483 29.145829165833163 28.125625125025007 23.374674934987 15-19 19.925 27.305 28.375 24.395 20-24 19.345000000000002 27.500000000000004 28.33 24.825 25-29 19.365 28.16 27.54 24.935 30-34 20.044999999999998 27.544999999999998 27.955000000000002 24.455 35-39 20.105 28.46 28.01 23.425 40-44 19.715 27.935 28.525 23.825 45-49 20.150000000000002 27.43 27.49 24.93 50-54 20.465 27.35 28.37 23.815 55-59 20.05 27.744999999999997 28.92 23.285 60-64 20.765 26.615 28.4 24.22 65-69 20.155 27.68 27.605 24.560000000000002 70-74 20.435 27.99 27.689999999999998 23.885 75-79 20.695 27.61 27.415 24.279999999999998 80-84 20.1 27.785 27.305 24.81 85-89 20.294999999999998 27.950000000000003 27.83 23.925 90-94 20.71 27.57 27.529999999999998 24.19 95-99 19.99 27.595 28.79 23.625 100-104 20.705000000000002 27.71 27.985 23.599999999999998 105-109 20.87 28.075 26.729999999999997 24.325 110-114 20.01 28.52 26.99 24.48 115-119 20.96 27.805000000000003 27.46 23.775 120-124 21.015 28.065 27.18 23.74 125-129 20.535 27.894999999999996 27.1 24.47 130-134 20.849999999999998 28.055000000000003 27.265 23.830000000000002 135-139 20.555 27.279999999999998 26.355 25.81 140-144 20.895 27.55 26.855 24.7 145-149 21.6 27.46 26.419999999999998 24.52 150-151 21.375 27.3625 24.975 26.2875 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.5 11 0.5 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 1.0 20 2.0 21 2.5 22 1.5 23 0.0 24 0.0 25 1.5 26 2.0 27 4.5 28 9.0 29 8.5 30 9.0 31 11.5 32 17.0 33 34.0 34 41.5 35 41.5 36 67.0 37 108.0 38 130.5 39 144.5 40 190.5 41 216.5 42 228.5 43 266.0 44 294.0 45 305.5 46 297.5 47 253.0 48 209.0 49 197.0 50 190.5 51 165.5 52 132.0 53 112.5 54 84.5 55 54.5 56 44.5 57 35.5 58 20.0 59 16.0 60 13.5 61 12.5 62 10.0 63 4.0 64 2.0 65 0.5 66 1.5 67 2.0 68 1.0 69 1.0 70 1.0 71 0.0 72 0.0 73 0.0 74 0.0 75 0.0 76 0.0 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.44999999999999996 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.02 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150-151 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 61.875 #Duplication Level Percentage of deduplicated Percentage of total 1 64.52525252525253 39.925 2 21.09090909090909 26.1 3 8.0 14.85 4 3.5555555555555554 8.799999999999999 5 1.3737373737373737 4.25 6 0.8484848484848486 3.15 7 0.40404040404040403 1.7500000000000002 8 0.0404040404040404 0.2 9 0.0808080808080808 0.44999999999999996 >10 0.0808080808080808 0.525 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GCAATCTATAAAATAGACCCACAGAATGCTAGGCCAGCAAATTCAAAATG 11 0.27499999999999997 No Hit CCTCACCACGTTATGGTATGAAGATCGCCTTATCTGAACAACACGGTGGT 10 0.25 No Hit CTAGGATCTTAACTGACGGAGTTTTTGAAGTCATGGAAAGCTTGTATGTT 9 0.22499999999999998 No Hit CAGGCAACCAACTCCAGAGTAATCTGAATATTAGCATTAGGTGGAACAGC 9 0.22499999999999998 No Hit CCAGGTTCAAATCCTCTTTCCCTTGTTTGCTTAATAGGAAACGCGCAGAT 8 0.2 No Hit ATTCAACAAAAACTATTCTCCATGAATTTTACAAATTCATTAAAATCTAT 7 0.17500000000000002 No Hit GCCACCGTATGAACAGGATTTGATGCTTGTTGCGTTGCCACTGATACCAT 7 0.17500000000000002 No Hit GCTCATTTTGAGAAGGGAGCTTGAAGTCGATGATCTTCCTGGTTAATGGA 7 0.17500000000000002 No Hit TCTCAAGGGGGTGACCATGGATCCCCATCCGCCGATCTATCATGCAGGAG 7 0.17500000000000002 No Hit TGAGAAGAAGAAGAGGGAACCTACTAAGGGGTCGTAGAAACCAACAACTT 7 0.17500000000000002 No Hit GCCAAGAATTCTGCAGCAGCCTCAGGGTCAGTAGTCTGTTCGCCCCTCAA 7 0.17500000000000002 No Hit GGGTTGCAAAGTACAGGCATTAGCGGACCAGATTGATTGTAATATAGACC 7 0.17500000000000002 No Hit CTTCCTTGGAGACCCTTCACTACACCAGCACCACAGATTGCACCAAGGCA 7 0.17500000000000002 No Hit GACTTCCTCGATTGCAGGTATAGCATTCACTCCAATTCTCTTCAAGGTGC 7 0.17500000000000002 No Hit CAGGACTAAAGGTAACACAACTGGAGGAGGTTATGCATACAAGAAAGCTA 7 0.17500000000000002 No Hit GCCTGCTGCAGTTCATCCTTCCTTGCTCCCACAACTTTGTCCACAATCTT 6 0.15 No Hit CATCCTCGTTAGCAAAGCAGAGGTCTATGTCCCCTGACTCCAGTAACTGT 6 0.15 No Hit CTCGGCGTCAAACTTGGTGTGGAGTGTGGATTTTTCCACTACTTTGAGGG 6 0.15 No Hit GTGGATAACAAACAACAGCTAAAGTCCCTGTCATTTGCAGCAGGGCAGCC 6 0.15 No Hit CTCCGCTGCTTCCGCCACCACATCCAACGCCGCCGATTTCTGGTTTCAGG 6 0.15 No Hit GTCATTGGTCTAGAATCCCAATTTTTTGGTGAAAGCAAAGCAGCATCAGA 6 0.15 No Hit CGTGGTTGATGACTAGAGCTAACCAGACTTGGGGAGGCATTGCTTGGGCT 6 0.15 No Hit GTCATCTAAAACACAGAAATTACAAGGCAAGGACATATACCATATCAACA 6 0.15 No Hit GTCTGACATCCCGGCCAAGCAAACTTTTACCATTAAAATTTAGAGCCTTC 6 0.15 No Hit CCTGTACAGTTCATTGTTTCTGTCACTGCAATACAGAAATGTTCTCATGC 6 0.15 No Hit TTCAAAACCATTCACATCAAGTTTCCCATTTACTGATTTCCCTTGCTTTC 6 0.15 No Hit TGGGTTTTGTTGTATAGATTGATCAACACTTCTCTCGCATTCGGGGCCAC 6 0.15 No Hit GTCAACAAATCCTTCCTTGTGCTCACCACGGTACTCCCCCTTGTGTTCAC 6 0.15 No Hit CTTCACTCCACCATCTCCTCCTCCTGCTCCTGCCGGACCGCTTCCACCAC 6 0.15 No Hit CTTTAAAGCGGTCGAACTAATGATATTTCTATCAGATGGTGATATGCTCA 6 0.15 No Hit CAATACAATCAGCTTAACCTTCATGTTCTGCAGCCACATTTTTCTCCGGA 6 0.15 No Hit CACAGCTGATAACCTTGATAAAAACATCTTCTGGCCCTGTATCTCTGAGA 6 0.15 No Hit CCCGTAACAGTTTTCGACGAATTAACACTGTCCAAACGAATGGCTAACAA 6 0.15 No Hit GGTCACTTCATTGAAGAAAAAGTCGAGCAAAAGGTAACCATTGATTACCA 6 0.15 No Hit CTGTACCAATGGTGAAACTCTTCTCAATTGGCATCTCGGCATCCTTGGAA 6 0.15 No Hit ACCGGATCTGAGCCCACTTGAAGAGTTTCCTCTTACCTCATCGGCAATAT 6 0.15 No Hit CAAGTATATCATCTTGCTGTATTCCACAAACTCCCATATACGGAGCTTGG 5 0.125 No Hit GTGAAAAAGACTGAGCTGCATTAACTTACAGAAAGAGGGATGGGCTTTGG 5 0.125 No Hit CTAAAACCCAAACTCCTGCCGAAACAGAAACCTGTCATTAGCTTCCCACC 5 0.125 No Hit CCGGTCCACGCTTCCCACTTACTTCTCCTCCACCAGACCCTTCTCCGGCT 5 0.125 No Hit CTCCATAATACATGCTAGGGTCTTGGGGAGCGGCGGCGGCGGCAGCAGCA 5 0.125 No Hit GGGAAACTGCTCCTTGATGGACTCAAGGAGTCTGACAGCAATGTCGAGAC 5 0.125 No Hit GTTCGTCCAGCCATTGGTCCCTCCCTAGCAAGCCCAAAGTCTGAAAGCTT 5 0.125 No Hit TTCCTCTTCAAGTTCTTTAATTAGCTTTTCAATCTGCCTCCTATAAACAG 5 0.125 No Hit CTGCAACCCAGAAATCCTAAACCCCAAACACAAGCTACTAGATTTCCTAT 5 0.125 No Hit CTGATTTTATCTCGATCACTTGAGAAGAAGGAGCAGGAGGGAAAGCAGAG 5 0.125 No Hit TCCACCCAATTCAATGACACAAACGTCAGCGGGACCAGGCTGTCCGTCAA 5 0.125 No Hit CCATTTCGACAACTTCCAGCTCCATCACCAGTCAATTTGGCGCCATAATC 5 0.125 No Hit TACCAATGTCCCAATTTATGGGATTCCCAATTAAAACCTTGGAGCAATAT 5 0.125 No Hit GCTCTGGCGAACCAAGGCATTTTACTTTACCAGCACGTTCATAAAAGGAG 5 0.125 No Hit GACAAGTCCAGTTGTGACAGCTGATGAGGTTGACAAGACATTCTTTCCAG 5 0.125 No Hit CACGCTAACAACCGTTCACTCCCACAAACCTAGTCTTATTCTTTATCTTC 5 0.125 No Hit AGCCACCTTAAGCTGCCCCCTCTTCGGAATCGGACGGCTAACAAAACCCC 5 0.125 No Hit CGAAAAGTTGAACTGCCAAAAGCAACCGTAACGACTGACTAGTAGTAGTA 5 0.125 No Hit CTGGAATAACACGTTGTAGACGTTCTTGCCTCCCCCCTCCCAGCTTATTA 5 0.125 No Hit ACACCACAAGTAACAAAGTCTAGCAATCCAGATGGTAAATAACAACGATA 5 0.125 No Hit TTCCAGCACTCATTGCAATCGAGAACATGGGAGTTATGCCATCTAAGCCT 5 0.125 No Hit CTAGAGATATTAGGACTACTTCAAAGTTCTCTCCTTTTTCCTTGAGCCTC 5 0.125 No Hit GCCTCAGAATCATTCTCCATACCTTCATCTGCCAAATCACAAATGGCCCG 5 0.125 No Hit GTCCACGACATCCTGACTGCTACTGGATAGACCGGAAATAGCTTTGAGAT 5 0.125 No Hit TGCTGATTCTTGCCAGACCATTGAACAGCATCCATTAATACATCCCACAA 5 0.125 No Hit CTCAGCTCGGCCGATTTGGCTGAGACGTCCGCAGCCGAGAAGCTGAAGAC 5 0.125 No Hit CATCTGTAATTTCAAGTGTTTTCTGAGCAAAAAAACCTTCAGAACCTCCT 5 0.125 No Hit GCCCTTTTGGCAGCAGGAGATTTGATAGACTTGGGCTGTTTAGGTTTAGC 5 0.125 No Hit ACCAAACCATTTAACATTCGGTATTCCAGTTCCTCCCTGCAGCATTTTGT 5 0.125 No Hit ATCATGTCCTAAGTTTTATGTCCAAGCCGTCCTGTTTGCTTTTTCTCTCT 5 0.125 No Hit GTTTCAAAAGCATGAGTATATCCGAAATTGAGGTCGTGAATACCCAGATT 5 0.125 No Hit CCAGACGTGCGTGATATAGCCCTGATCCACCCTTGGACAAACTCCCATAG 5 0.125 No Hit TTGAGAATCAGCCATGGATTTGATTGCACTGCAGCTCGATCGCAGCGAGT 5 0.125 No Hit CAACGGAAATTTGTCACTCGCATAACAAAAATTAAAGCATAAATATCCAA 5 0.125 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.1 0.0 0.0 0.0 0.0 62-63 0.1 0.0 0.0 0.0 0.0 64-65 0.1 0.0 0.0 0.0 0.0 66-67 0.1 0.0 0.0 0.0 0.0 68-69 0.1 0.0 0.0 0.0 0.0 70-71 0.1 0.0 0.0 0.0 0.0 72-73 0.1125 0.0 0.0 0.0 0.0 74-75 0.125 0.0 0.0 0.0 0.0 76-77 0.15 0.0 0.0 0.0 0.0 78-79 0.2125 0.0 0.0 0.0 0.0 80-81 0.2625 0.0 0.0 0.0 0.0 82-83 0.375 0.0 0.0 0.0 0.0 84-85 0.5 0.0 0.0 0.0 0.0 86-87 0.55 0.0 0.0 0.0 0.0 88-89 0.6125 0.0 0.0 0.0 0.0 90-91 0.75 0.0 0.0 0.0 0.0 92-93 0.9375 0.0 0.0 0.0 0.0 94-95 1.1625 0.0 0.0 0.0 0.0 96-97 1.3375 0.0 0.0 0.0 0.0 98-99 1.6875 0.0 0.0 0.0 0.0 100-101 1.9500000000000002 0.0 0.0 0.0 0.0 102-103 2.2249999999999996 0.0 0.0 0.0 0.0 104-105 2.4375 0.0 0.0 0.0 0.0 106-107 2.5625 0.0 0.0 0.0 0.0 108-109 2.9875 0.0 0.0 0.0 0.0 110-111 3.3625 0.0 0.0 0.0 0.0 112-113 3.9000000000000004 0.0 0.0 0.0 0.0 114-115 4.2875 0.0 0.0 0.0 0.0 116-117 4.775 0.0 0.0 0.0 0.0 118-119 5.65 0.0 0.0 0.0 0.0 120-121 6.1 0.0 0.0 0.0 0.0 122-123 6.762499999999999 0.0 0.0 0.0 0.0 124-125 7.5 0.0 0.0 0.0 0.0 126-127 8.375 0.0 0.0 0.0 0.0 128-129 9.0625 0.0 0.0 0.0 0.0 130-131 9.7375 0.0 0.0 0.0 0.0 132-133 10.3625 0.0 0.0 0.0 0.0 134-135 11.287500000000001 0.0 0.0 0.0 0.0 136-137 11.899999999999999 0.0 0.0 0.0 0.0 138-139 12.712499999999999 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AACCTGA 10 0.006830828 145.0 145 GATTTAA 10 0.006830828 145.0 6 AGATTTA 10 0.006830828 145.0 5 ATTTAAA 10 0.006830828 145.0 7 >>END_MODULE SRR26075369 read2 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR26075369_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.3535 37.0 37.0 37.0 37.0 37.0 2 36.464 37.0 37.0 37.0 37.0 37.0 3 36.414 37.0 37.0 37.0 37.0 37.0 4 36.4835 37.0 37.0 37.0 37.0 37.0 5 36.5055 37.0 37.0 37.0 37.0 37.0 6 36.4105 37.0 37.0 37.0 37.0 37.0 7 36.3785 37.0 37.0 37.0 37.0 37.0 8 36.4395 37.0 37.0 37.0 37.0 37.0 9 36.476 37.0 37.0 37.0 37.0 37.0 10-14 36.44349999999999 37.0 37.0 37.0 37.0 37.0 15-19 36.3986 37.0 37.0 37.0 37.0 37.0 20-24 36.3922 37.0 37.0 37.0 37.0 37.0 25-29 36.293600000000005 37.0 37.0 37.0 37.0 37.0 30-34 36.2223 37.0 37.0 37.0 37.0 37.0 35-39 36.187200000000004 37.0 37.0 37.0 37.0 37.0 40-44 36.1408 37.0 37.0 37.0 37.0 37.0 45-49 36.104299999999995 37.0 37.0 37.0 37.0 37.0 50-54 35.989999999999995 37.0 37.0 37.0 37.0 37.0 55-59 36.050599999999996 37.0 37.0 37.0 37.0 37.0 60-64 36.137 37.0 37.0 37.0 37.0 37.0 65-69 36.049400000000006 37.0 37.0 37.0 37.0 37.0 70-74 35.9468 37.0 37.0 37.0 37.0 37.0 75-79 35.912099999999995 37.0 37.0 37.0 37.0 37.0 80-84 35.9396 37.0 37.0 37.0 37.0 37.0 85-89 35.949799999999996 37.0 37.0 37.0 37.0 37.0 90-94 35.80460000000001 37.0 37.0 37.0 37.0 37.0 95-99 35.8732 37.0 37.0 37.0 37.0 37.0 100-104 35.74239999999999 37.0 37.0 37.0 37.0 37.0 105-109 35.733 37.0 37.0 37.0 37.0 37.0 110-114 35.6314 37.0 37.0 37.0 37.0 37.0 115-119 35.6205 37.0 37.0 37.0 37.0 37.0 120-124 35.5308 37.0 37.0 37.0 37.0 37.0 125-129 35.485 37.0 37.0 37.0 37.0 37.0 130-134 35.455499999999994 37.0 37.0 37.0 37.0 37.0 135-139 35.2969 37.0 37.0 37.0 29.8 37.0 140-144 35.2401 37.0 37.0 37.0 29.8 37.0 145-149 35.2296 37.0 37.0 37.0 27.4 37.0 150-151 34.8275 37.0 37.0 37.0 25.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.0 1101 2 0.0 1101 3 0.0 1101 4 0.0 1101 5 0.0 1101 6 0.0 1101 7 0.0 1101 8 0.0 1101 9 0.0 1101 10-14 0.0 1101 15-19 0.0 1101 20-24 0.0 1101 25-29 0.0 1101 30-34 0.0 1101 35-39 0.0 1101 40-44 0.0 1101 45-49 0.0 1101 50-54 0.0 1101 55-59 0.0 1101 60-64 0.0 1101 65-69 0.0 1101 70-74 0.0 1101 75-79 0.0 1101 80-84 0.0 1101 85-89 0.0 1101 90-94 0.0 1101 95-99 0.0 1101 100-104 0.0 1101 105-109 0.0 1101 110-114 0.0 1101 115-119 0.0 1101 120-124 0.0 1101 125-129 0.0 1101 130-134 0.0 1101 135-139 0.0 1101 140-144 0.0 1101 145-149 0.0 1101 150-151 0.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 13 1.0 14 2.0 15 0.0 16 3.0 17 4.0 18 4.0 19 3.0 20 0.0 21 2.0 22 2.0 23 9.0 24 3.0 25 15.0 26 9.0 27 7.0 28 11.0 29 14.0 30 30.0 31 27.0 32 46.0 33 81.0 34 171.0 35 633.0 36 2703.0 37 220.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 36.85 21.95 11.125 30.075000000000003 2 25.3 27.925 29.9 16.875 3 21.725 28.825 28.7 20.75 4 24.474999999999998 33.650000000000006 23.425 18.45 5 25.650000000000002 36.675000000000004 22.35 15.325 6 20.849999999999998 39.65 23.05 16.45 7 20.549999999999997 21.825 37.025000000000006 20.599999999999998 8 20.125 25.55 29.349999999999998 24.975 9 22.525000000000002 24.7 30.525000000000002 22.25 10-14 24.185000000000002 28.62 25.724999999999998 21.47 15-19 23.055 29.299999999999997 27.49 20.155 20-24 23.65 29.64 26.505000000000003 20.205000000000002 25-29 24.445 27.994999999999997 26.875 20.685000000000002 30-34 23.435 28.725 26.96 20.880000000000003 35-39 23.98 28.68 27.345000000000002 19.994999999999997 40-44 24.38 29.154999999999998 26.31 20.155 45-49 23.52 28.51 27.139999999999997 20.830000000000002 50-54 23.955000000000002 28.199999999999996 27.1 20.745 55-59 24.104999999999997 29.225 26.169999999999998 20.5 60-64 24.63 28.525 26.479999999999997 20.365 65-69 23.810000000000002 27.555000000000003 27.985 20.65 70-74 24.58 28.144999999999996 27.005000000000003 20.27 75-79 24.2 27.495000000000005 27.325 20.979999999999997 80-84 23.955000000000002 28.849999999999998 27.41 19.785 85-89 23.925 27.71 27.060000000000002 21.305 90-94 24.55 27.665 26.91 20.875 95-99 24.685000000000002 27.900000000000002 26.950000000000003 20.465 100-104 24.385 28.910000000000004 26.43 20.275000000000002 105-109 23.965 28.33 27.16 20.544999999999998 110-114 24.884999999999998 28.299999999999997 27.24 19.575 115-119 25.495 27.685 27.310000000000002 19.509999999999998 120-124 24.695 28.34 26.91 20.055 125-129 25.16 28.325 26.834999999999997 19.68 130-134 25.085 27.51 26.950000000000003 20.455000000000002 135-139 26.195 28.525 25.85 19.43 140-144 26.565 27.98 25.619999999999997 19.835 145-149 26.695 27.860000000000003 26.790000000000003 18.655 150-151 26.0125 28.0625 26.887499999999996 19.037499999999998 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 0.0 1 0.0 2 0.5 3 0.5 4 0.0 5 0.5 6 1.0 7 1.5 8 1.5 9 0.5 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.5 16 0.5 17 1.5 18 1.5 19 0.0 20 0.0 21 0.0 22 0.5 23 0.5 24 0.5 25 2.0 26 2.0 27 2.0 28 3.0 29 3.0 30 5.0 31 18.0 32 23.5 33 20.5 34 33.0 35 53.5 36 73.0 37 95.5 38 125.0 39 153.5 40 171.5 41 213.0 42 263.0 43 286.0 44 284.0 45 285.0 46 323.0 47 293.5 48 243.0 49 214.5 50 168.5 51 150.5 52 116.5 53 75.5 54 58.5 55 53.5 56 48.5 57 33.5 58 22.0 59 16.5 60 8.0 61 6.5 62 7.5 63 6.5 64 5.0 65 2.5 66 2.0 67 2.0 68 1.5 69 0.5 70 0.0 71 0.5 72 0.5 73 0.0 74 0.0 75 0.0 76 0.5 77 0.5 78 0.0 79 0.0 80 0.0 81 0.5 82 1.0 83 0.5 84 0.5 85 0.5 86 0.5 87 1.0 88 0.5 89 0.0 90 0.0 91 0.5 92 0.5 93 0.0 94 0.5 95 1.0 96 0.5 97 1.0 98 1.0 99 1.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150-151 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 62.575 #Duplication Level Percentage of deduplicated Percentage of total 1 65.4814222932481 40.975 2 20.854974031162605 26.1 3 7.471034758290052 14.025000000000002 4 3.4758290051937673 8.7 5 1.238513783459848 3.875 6 0.8789452656811825 3.3000000000000003 7 0.3595685177786656 1.575 8 0.0 0.0 9 0.11985617259288853 0.675 >10 0.11985617259288853 0.775 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTAAGATGGTTCACTTTACAATGTTTTAGTTTGATCTATTTTCTGTTATT 11 0.27499999999999997 No Hit CTGAGATATGCAATATTCAATTTGGAAGTTATTCAAGCAGCTGTCCGGAA 10 0.25 No Hit TGAGTTCATTTGGGCAAATGGGGAACCACGACATGGGTCCACCATGGTTG 10 0.25 No Hit GACGGAGTAGAGTTCACTGTTAAGGATGGTCATTTCTGTCCTGCACTGGC 9 0.22499999999999998 No Hit CCCATCTCTATAAGCTTAAACTCTCTGCCTGTGGGCAACAGAATCTAGAA 9 0.22499999999999998 No Hit GTCTGGGGGATCTTAATTACCCATCGTTCTCTGTTCCACTAGAAACTGCT 9 0.22499999999999998 No Hit AGCACTTCGGCCGGGCCAAGACGCGGCCACCAGTTATGAAGGTTCCCTAT 7 0.17500000000000002 No Hit CAATGTTTTTGACCACAACAGAGATGGTTTTGTTGATGCAAGTGAGTTGC 7 0.17500000000000002 No Hit CACGAATCCAAGGGTTGTTGTTGGTGTCATGCTTTCTAACCGTTTCTGCT 7 0.17500000000000002 No Hit CATGATCTTTGCCCTTGTTTACTGCACTGCTGGTATCTCAGGTGGACACA 7 0.17500000000000002 No Hit GAAAAAACACTCTCCTACCTATCTCCCAAAACCCAATAAACAAACAAAGA 7 0.17500000000000002 No Hit AAATGGCGGTGAAGATTCGTTTAGCAAGACTGGGATGCAAAAACAGGGCA 7 0.17500000000000002 No Hit CTCATTGGTCTAGGAGGTCTCTATGGGGCAACTAATCTCAGCGATCCATT 7 0.17500000000000002 No Hit TGCTTGCTCTGGCATTTCTTGGATTTGTATTCTCCATTTTCGGGATGCAG 7 0.17500000000000002 No Hit GTTCAGACTGGCATAAAACTGATCTTAAACTTATGCTTAGCTGATGGGTT 7 0.17500000000000002 No Hit GTTGCAGGCATAACTAGTAGTGATCTTGAAGGCATAAGTACTGATATTGG 6 0.15 No Hit CGGCACTCGACTTCCATAAGTATAAGTCACAGCTCAGCTGATCTTAGGCA 6 0.15 No Hit CCCAAAAGACCACTACTCTTCCTCTCTATAATTTTCTAGGGTTTAGCAAT 6 0.15 No Hit CGGGCACACCATGATGCTTAATGTGAAGAAACCACCGGTTAGTTCCAGAG 6 0.15 No Hit TAAATTGATCATGCATTCTATAATAGCTGGAAGCAGTGGGAACGGTAGTG 6 0.15 No Hit GGAGGAGGTTCGGAGAGAGAACCAGTGGGCATTTGAGTAGAAGGAAAGAA 6 0.15 No Hit ATTTTTTCTTGGGTGTGTGTTCTTGCTTTGGTTAGCTAAGAAAGATTATT 6 0.15 No Hit CGGCAATTCAACACGTTCAATTTTGTTTGAATCATTTTGCGGCCGATGTT 6 0.15 No Hit GGCACATAAGGGTCCTAATTTATCGCTAGTGATGGCACCATCAAGTAGTT 6 0.15 No Hit GCCAGACCAAAGCCAGTGGAACGGTGGTGGATATTATGGATATCCACAAG 6 0.15 No Hit CGTATCTTTCTTCTGTGATAATAACCTCCGCGTCTGTTTCCTTTGAAAGT 6 0.15 No Hit AACTGTTTCTGGAATTAGTGGTACCGGGAAGAAGAAAATCCTGCTTCTGA 6 0.15 No Hit CAGAGGATGAGGAAGATAGCGAGGATGAAAAGCCAGCGAAGACTCCAAAG 6 0.15 No Hit AGCTGAGAAGCTTGATTGCCGAGAAGAGCTGCGCTCCTCTCATGCTTCGT 6 0.15 No Hit CTCGAAGCTGTGCTATCATCCCAACTATGATTGTTGCACTTGTTTTTGAT 6 0.15 No Hit AGACCATCTCACTCTTGCTTGTTATGTAGACAGTGGAGCTCAGGAAAGCA 6 0.15 No Hit CTCCATCTCTCTCTCTTAGCCTCATTGTTTCAAGAAAATGGGTAGCCTTG 6 0.15 No Hit ATTCCTCAAGTACCAGCGAGAGGCCTTGATTACCTGTTTAGAGATCGCTG 6 0.15 No Hit GTTCCTCATTTCTCTCCAAAACCCGAAAGAGAACACAACACTGAGAGAGA 6 0.15 No Hit AGAAAAGGAAAGACACCATGGCAGGAATCATGCACAAGATTGAGGAGACC 6 0.15 No Hit CATGGAGCTACCACTATCTGCACCACCTCTATCGTCATCACAAAAACCAC 6 0.15 No Hit CACAGCTCAGTGTGTTTGCATGGTTGATGAATTAGGTAATCGTACTATGC 6 0.15 No Hit AAGTGACAGGCATGCTTCTGGAGATGGATCAGACTGAGGTTCTGCACTTG 5 0.125 No Hit GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG 5 0.125 No Hit GAAAATCATAAACGACTTCACTCATCTGGTCAATCAAGTTGAACCCTTGA 5 0.125 No Hit GTTTTGTGGAGCTGGCAGATATGACCGAGGAGGAATGGACCCTGTTTATG 5 0.125 No Hit GGAAAATGCTATTGTTCATGAAGTTATGCAAGATAGTCCAAAAAGTCCAG 5 0.125 No Hit GTTTTAAAGGAGGGTTTGGATCCCTTGGAAAGACAAGTGAGAGAGGTGTT 5 0.125 No Hit CCACACCTATGGTCAGCCCTTGGCTCACTTCCTCAGGGCAACTGCTAAAG 5 0.125 No Hit AGCGGATCCTTTGGAGAGATCTATCTCGGTGCTAATATTCAGACCAACGA 5 0.125 No Hit CTGAATGATTCTGAGAATGTTTTGGTTGCTGAAACTAAAGATGCATCAGA 5 0.125 No Hit CCTTCTCTGGCAACTGAGCAGGCTTCAATGGATTGGAAGTGGCACATCTG 5 0.125 No Hit CCAGAACAATACAACAGGCTGTTGGAGTTGTGGGTTGCATTATCATGCCT 5 0.125 No Hit CCTAGATCAATACAGGAGAAATCGGTCACTTACTAGAGGAGAGATCGATG 5 0.125 No Hit CTTGATAGTGCAGAGAGTGAAGGTGATACCTCCACCTCGAGTGGTTCTGA 5 0.125 No Hit GCTGAGCTCAAAGTACTTGAAGGCTGCTCAAGAGCTACTTGATGAGGTTG 5 0.125 No Hit GATGTTGTCTCTGGTCGTTCGCATCCCTCGGTTATGGATGTTAAGATTGG 5 0.125 No Hit AGCCAAGGACTCCTAGAGAAAAGAAGCCTAGACAACCTAAACCAAAAGCT 5 0.125 No Hit CTGGAAGACAAGATTACAAATAATACTTGGAGCAGCTCAAGGATTAGCTT 5 0.125 No Hit GAATACTTCAGAGATATGGGCTACAATGTGAGCATGATGGCAGATTCAAC 5 0.125 No Hit GATCTCTACAAGATAACGAATCTTGATGCCCCGCAAAAAAATCTTATCAT 5 0.125 No Hit GAACTTTTGGACAGTAAAGTTATCGCTGAAACAATTGTTTTGAAGCTCCA 5 0.125 No Hit ATTACGTGAAAGGCGTTGTCGGGCTAGCCAAAGGCTTAAGAAAGGTCAAA 5 0.125 No Hit AGTAATTGAAGTTCCACAGGTTGAGGATTGTCTTCAACCTGTAATTAATA 5 0.125 No Hit GGGAACAAGACACAAGAACAAGAACAAGAACAAGAATGGAGGAACTCCCT 5 0.125 No Hit TTTTTTTGTTTCAGGGTGTTATCACTTGGTGTTTTAAGTTCAGCTCCATG 5 0.125 No Hit AGGAAATGAAATCTGCATTAACAACTCAGATAGCAGAGGAAGCTGACATA 5 0.125 No Hit GTATGTCCTAGATGATGGTGGAGAGGTGGACCTGGACCTTGGAAATTATG 5 0.125 No Hit CTGGAGATCAACTATCCTAGTGCCATGACGGCTACTCCTCCTCTATCCGC 5 0.125 No Hit GTATGAGACCAAAGTTGGTGAAGAGAGTGGTGCTGTTGAGACCAAGGATC 5 0.125 No Hit GTTCTACCTCCACTGCTGCCTCTATTGCAAGAAAGGTTTGATCTTTCTCA 5 0.125 No Hit CTCATAAACCCTGAGATATGTTAAGCTTGTCTGCTGCTCCCACCACAGCC 5 0.125 No Hit AAGTTATTCTTGCTTCCTTGGATGGATTTATCAAGGTGTGTGATGCTATT 5 0.125 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.1 0.0 0.0 0.0 0.0 62-63 0.1 0.0 0.0 0.0 0.0 64-65 0.1 0.0 0.0 0.0 0.0 66-67 0.1 0.0 0.0 0.0 0.0 68-69 0.1 0.0 0.0 0.0 0.0 70-71 0.1 0.0 0.0 0.0 0.0 72-73 0.1375 0.0 0.0 0.0 0.0 74-75 0.15 0.0 0.0 0.0 0.0 76-77 0.175 0.0 0.0 0.0 0.0 78-79 0.23750000000000002 0.0 0.0 0.0 0.0 80-81 0.2875 0.0 0.0 0.0 0.0 82-83 0.4 0.0 0.0 0.0 0.0 84-85 0.525 0.0 0.0 0.0 0.0 86-87 0.575 0.0 0.0 0.0 0.0 88-89 0.6375 0.0 0.0 0.0 0.0 90-91 0.775 0.0 0.0 0.0 0.0 92-93 0.9375 0.0 0.0 0.0 0.0 94-95 1.1625 0.0 0.0 0.0 0.0 96-97 1.3375 0.0 0.0 0.0 0.0 98-99 1.6875 0.0 0.0 0.0 0.0 100-101 1.9500000000000002 0.0 0.0 0.0 0.0 102-103 2.2249999999999996 0.0 0.0 0.0 0.0 104-105 2.4375 0.0 0.0 0.0 0.0 106-107 2.5625 0.0 0.0 0.0 0.0 108-109 2.9625 0.0 0.0 0.0 0.0 110-111 3.35 0.0 0.0 0.0 0.0 112-113 3.9000000000000004 0.0 0.0 0.0 0.0 114-115 4.300000000000001 0.0 0.0 0.0 0.0 116-117 4.7875 0.0 0.0 0.0 0.0 118-119 5.6625 0.0 0.0 0.0 0.0 120-121 6.1625 0.0 0.0 0.0 0.0 122-123 6.875 0.0 0.0 0.0 0.0 124-125 7.6875 0.0 0.0 0.0 0.0 126-127 8.6 0.0 0.0 0.0 0.0 128-129 9.3125 0.0 0.0 0.0 0.0 130-131 9.9625 0.0 0.0 0.0 0.0 132-133 10.5875 0.0 0.0 0.0 0.0 134-135 11.5125 0.0 0.0 0.0 0.0 136-137 12.125 0.0 0.0 0.0 0.0 138-139 12.95 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CACACTC 10 0.006830828 145.0 1 ACACTCT 10 0.006830828 145.0 2 GTTTTAA 10 0.006830828 145.0 145 TTCTCTA 10 0.006830828 145.0 8 GGTCGGA 10 0.006830828 145.0 1 >>END_MODULE Read 3756983 spots for SRR26075369.sra Written 3756983 spots for SRR26075369.sra Read 3756983 spots for SRR26075369.sra Written 3756983 spots for SRR26075369.sra Read 3756983 spots for SRR26075369.sra Written 3756983 spots for SRR26075369.sra Read 3756983 spots for SRR26075369.sra Written 3756983 spots for SRR26075369.sra Read 3756983 spots for SRR26075369.sra Written 3756983 spots for SRR26075369.sra Read 3756983 spots for SRR26075369.sra Written 3756983 spots for SRR26075369.sra Read 3756983 spots for SRR26075369.sra Written 3756983 spots for SRR26075369.sra Read 3756983 spots for SRR26075369.sra Written 3756983 spots for SRR26075369.sra Read 3756983 spots for SRR26075369.sra Written 3756983 spots for SRR26075369.sra Read 3756983 spots for SRR26075369.sra Written 3756983 spots for SRR26075369.sra Read 3756983 spots for SRR26075369.sra Written 3756983 spots for SRR26075369.sra Read 3756983 spots for SRR26075369.sra Written 3756983 spots for SRR26075369.sra Read 3756983 spots for SRR26075369.sra Written 3756983 spots for SRR26075369.sra Read 3756983 spots for SRR26075369.sra Written 3756983 spots for SRR26075369.sra Read 3756983 spots for SRR26075369.sra Written 3756983 spots for SRR26075369.sra Read 3756985 spots for SRR26075369.sra Written 3756985 spots for SRR26075369.sra Read 3756983 spots for SRR26075369.sra Written 3756983 spots for SRR26075369.sra Read 3756983 spots for SRR26075369.sra Written 3756983 spots for SRR26075369.sra Read 3756983 spots for SRR26075369.sra Written 3756983 spots for SRR26075369.sra Read 3756983 spots for SRR26075369.sra Written 3756983 spots for SRR26075369.sra SRR ids: ['SRR26075369.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_0gpb3cu5 SRR26075369.sra spots: 75139662 blocks: [[1, 3756983], [3756984, 7513966], [7513967, 11270949], [11270950, 15027932], [15027933, 18784915], [18784916, 22541898], [22541899, 26298881], [26298882, 30055864], [30055865, 33812847], [33812848, 37569830], [37569831, 41326813], [41326814, 45083796], [45083797, 48840779], [48840780, 52597762], [52597763, 56354745], [56354746, 60111728], [60111729, 63868711], [63868712, 67625694], [67625695, 71382677], [71382678, 75139662]] SRR26075369 file size 27760406 SRR26075369 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o SRR26075369 SRR26075369_1.fastq SRR26075369_2.fastq Input file: SRR26075369_1.fastq Paired file: SRR26075369_2.fastq trimmed: SRR26075369-trimmed-pair1.fastq, SRR26075369-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Tue Feb 11 22:50:12 2025 >> started Tue Feb 11 22:51:40 2025 >> done (88.370s) 75139662 read pairs processed; of these: 329 ( 0.00%) short read pairs filtered out after trimming by size control 76630 ( 0.10%) empty read pairs filtered out after trimming by size control 75062703 (99.90%) read pairs available; of these: 12508787 (16.66%) trimmed read pairs available after processing 62553916 (83.34%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 18 30 0.00% 19 42 0.00% 20 25 0.00% 21 29 0.00% 22 37 0.00% 23 40 0.00% 24 34 0.00% 25 43 0.00% 26 62 0.00% 27 57 0.00% 28 70 0.00% 29 60 0.00% 30 85 0.00% 31 82 0.00% 32 122 0.00% 33 93 0.00% 34 85 0.00% 35 107 0.00% 36 88 0.00% 37 109 0.00% 38 131 0.00% 39 143 0.00% 40 145 0.00% 41 138 0.00% 42 143 0.00% 43 215 0.00% 44 185 0.00% 45 218 0.00% 46 275 0.00% 47 270 0.00% 48 323 0.00% 49 423 0.00% 50 504 0.00% 51 533 0.00% 52 624 0.00% 53 805 0.00% 54 790 0.00% 55 1023 0.00% 56 980 0.00% 57 1204 0.00% 58 1561 0.00% 59 1782 0.00% 60 2150 0.00% 61 2350 0.00% 62 2808 0.00% 63 3399 0.00% 64 3930 0.01% 65 4635 0.01% 66 5218 0.01% 67 5707 0.01% 68 6634 0.01% 69 7608 0.01% 70 9203 0.01% 71 10304 0.01% 72 11947 0.02% 73 13696 0.02% 74 15275 0.02% 75 17202 0.02% 76 19410 0.03% 77 20928 0.03% 78 22859 0.03% 79 25778 0.03% 80 27829 0.04% 81 30659 0.04% 82 33889 0.05% 83 37372 0.05% 84 40984 0.05% 85 43946 0.06% 86 47603 0.06% 87 51008 0.07% 88 54022 0.07% 89 55639 0.07% 90 60494 0.08% 91 64138 0.09% 92 67848 0.09% 93 71810 0.10% 94 76372 0.10% 95 81083 0.11% 96 86621 0.12% 97 90774 0.12% 98 94543 0.13% 99 96823 0.13% 100 100097 0.13% 101 104792 0.14% 102 108130 0.14% 103 114109 0.15% 104 117971 0.16% 105 123474 0.16% 106 129295 0.17% 107 134623 0.18% 108 137214 0.18% 109 141830 0.19% 110 143234 0.19% 111 147585 0.20% 112 154619 0.21% 113 156263 0.21% 114 161674 0.22% 115 168788 0.22% 116 174453 0.23% 117 179351 0.24% 118 187644 0.25% 119 188863 0.25% 120 192827 0.26% 121 196727 0.26% 122 200883 0.27% 123 204751 0.27% 124 210215 0.28% 125 216659 0.29% 126 222159 0.30% 127 228006 0.30% 128 233398 0.31% 129 238987 0.32% 130 243765 0.32% 131 247220 0.33% 132 250765 0.33% 133 255270 0.34% 134 256304 0.34% 135 264125 0.35% 136 269831 0.36% 137 272319 0.36% 138 279935 0.37% 139 286942 0.38% 140 291503 0.39% 141 295891 0.39% 142 300834 0.40% 143 299927 0.40% 144 307860 0.41% 145 311011 0.41% 146 312852 0.42% 147 319099 0.43% 148 324856 0.43% 149 327641 0.44% 150 334003 0.44% 151 62553916 83.34% 75062703 reads passed initial QC criterion=sequence-density sequence-density=0.26 sequence-density-rank=1 fanout-score=3.15 fanout-score-rank=24 prefix-density=0.38 prefix-fanout=2.1 sequence=CACTTGCAGCCATTCTC criterion=fanout-score sequence-density=0.02 sequence-density-rank=34 fanout-score=169.63 fanout-score-rank=1 prefix-density=0.32 prefix-fanout=11.5 sequence=CATCACCATCCCTGATTGATCTTTGATTCACAACAAGACCAACCAACCGTACAATGTTTACATAACTTGGGATAATCTCACAAGAAAGAAAGATCAATACAAAACTAGTCAGCTCAGGATGTTCCAGGCACGGTAGAACCGTAGAACCATAACAACAAGAGACATATTGCAGATGAGTACTGAAAAACAAAACACAGTACGTATTTACATGGGCAACCTTGGTTGAAGGCAACCTCATCAACGATGCTCGCTCTTCGTCTCTCCACTGTACATCCAGTCATAGACAGTGGGTGTGTTAGGCTGTGGCTTGTCAAAAACATGAGCACCAATATTCTTAGTAGCAAGGTTGCTACCAGGGTGGAACACGCTCCTCCAAACATTGCTACGCGCCGACACTGGGGTTGTAGGGGTCACTGGTGTCGTCGGTGTCCCTGGAGTTCCTGGCATAGTCATGGACCTCTGAAACTTATTAACAGGACTGCTCCCCTCTCCGACGTCAATATCTTTGATG criterion=sequence-density sequence-density=0.32 sequence-density-rank=1 fanout-score=2.77 fanout-score-rank=26 prefix-density=0.32 prefix-fanout=2.7 sequence=ATGTACCCTGAC criterion=fanout-score sequence-density=0.10 sequence-density-rank=21 fanout-score=337.58 fanout-score-rank=1 prefix-density=1.05 prefix-fanout=32.3 sequence=AAGAAGAAGAAA SRR26075369 testing PE reads STAR mapping to Ensembl genome Started job on | Feb 11 22:52:21 Started mapping on | Feb 11 22:52:21 Finished on | Feb 11 23:03:26 Mapping speed, Million of reads per hour | 406.35 Number of input reads | 75062703 Average input read length | 293 UNIQUE READS: Uniquely mapped reads number | 68023892 Uniquely mapped reads % | 90.62% Average mapped length | 291.85 Number of splices: Total | 62755812 Number of splices: Annotated (sjdb) | 61152476 Number of splices: GT/AG | 61603574 Number of splices: GC/AG | 879586 Number of splices: AT/AC | 60416 Number of splices: Non-canonical | 212236 Mismatch rate per base, % | 0.39% Deletion rate per base | 0.03% Deletion average length | 3.12 Insertion rate per base | 0.02% Insertion average length | 2.54 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 2495013 % of reads mapped to multiple loci | 3.32% Number of reads mapped to too many loci | 437053 % of reads mapped to too many loci | 0.58% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 5.23% % of reads unmapped: other | 0.24% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 4543798 4543798 4543798 N_multimapping 2495013 2495013 2495013 N_noFeature 1799728 67379548 2130289 N_ambiguous 784493 4069 468195 UnstrandedReadsAssigned:65439671 PositiveStrandReadsAssigned:640275 NegativeStrandReadsAssigned:65425408 Dataset is classified negative stranded MeadianReadLen=151 20thPercentileLength=151 echo kmer=147 SRR26075369 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,400 [index] number of k-mers: 62,057,036 [index] number of equivalence classes: 130,681 [quant] running in paired-end mode [quant] will process pair 1: SRR26075369-trimmed-pair1.fastq SRR26075369-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 75,062,703 reads, 66,563,590 reads pseudoaligned [quant] estimated average fragment length: 209.893 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,306 rounds 52401 SRR26075369.ke.tsv 34699 SRR26075369.se.tsv 87100 total ==> SRR26075369.ke.tsv <== target_id length eff_length est_counts tpm Potri.005G200100.1.v4.1 2018 1809.11 9757 78.0723 Potri.005G024800.1.v4.1 1035 826.107 4537 79.5019 Potri.004G059700.1.v4.1 961 752.149 5 0.0962301 Potri.007G009000.2.v4.1 1416 1207.11 0 0 Potri.003G141000.2.v4.1 2943 2734.11 3039.09 16.0906 Potri.016G087400.1.v4.1 270 91.6096 4462 705.072 Potri.015G069301.1.v4.1 564 356.967 0 0 Potri.010G195200.1.v4.1 1773 1564.11 2250 20.8239 Potri.012G127500.1.v4.1 977 768.107 32775 617.684 ==> SRR26075369.se.tsv <== Potri.001G166300.v4.1 0 Potri.001G448400.v4.1 771 Potri.001G233950.v4.1 0 Potri.001G122700.v4.1 909 Potri.001G212900.v4.1 0 Potri.001G182400.v4.1 0 Potri.001G256600.v4.1 0 Potri.001G040500.v4.1 0 Potri.001G416900.v4.1 0 Potri.001G452600.v4.1 2148 SRR26075369 completed mapping pipeline successfully