Starting /dee2/code/volunteer_pipeline.sh SRR26075370 current disk space = 3051968135168 free memory = 1576202136 SRR26075370 SRAfilesize fa56214bb882b53b2adf228a39393e63 SRR26075370.sra SRR26075370.sra file validated SRR26075370 is paired end SRR26075370 is conventional basespace SRR26075370 read1 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR26075370_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.624 37.0 37.0 37.0 37.0 37.0 2 36.5245 37.0 37.0 37.0 37.0 37.0 3 36.4125 37.0 37.0 37.0 37.0 37.0 4 36.6405 37.0 37.0 37.0 37.0 37.0 5 36.696 37.0 37.0 37.0 37.0 37.0 6 36.5845 37.0 37.0 37.0 37.0 37.0 7 36.567 37.0 37.0 37.0 37.0 37.0 8 36.642 37.0 37.0 37.0 37.0 37.0 9 36.6475 37.0 37.0 37.0 37.0 37.0 10-14 36.650349999999996 37.0 37.0 37.0 37.0 37.0 15-19 36.5612 37.0 37.0 37.0 37.0 37.0 20-24 36.5462 37.0 37.0 37.0 37.0 37.0 25-29 36.484899999999996 37.0 37.0 37.0 37.0 37.0 30-34 36.4041 37.0 37.0 37.0 37.0 37.0 35-39 36.3246 37.0 37.0 37.0 37.0 37.0 40-44 36.249700000000004 37.0 37.0 37.0 37.0 37.0 45-49 36.173899999999996 37.0 37.0 37.0 37.0 37.0 50-54 36.1245 37.0 37.0 37.0 37.0 37.0 55-59 36.071000000000005 37.0 37.0 37.0 37.0 37.0 60-64 35.995900000000006 37.0 37.0 37.0 37.0 37.0 65-69 35.8497 37.0 37.0 37.0 37.0 37.0 70-74 35.953700000000005 37.0 37.0 37.0 37.0 37.0 75-79 35.9582 37.0 37.0 37.0 37.0 37.0 80-84 35.866200000000006 37.0 37.0 37.0 37.0 37.0 85-89 35.7654 37.0 37.0 37.0 37.0 37.0 90-94 35.7562 37.0 37.0 37.0 37.0 37.0 95-99 35.716300000000004 37.0 37.0 37.0 37.0 37.0 100-104 35.6554 37.0 37.0 37.0 37.0 37.0 105-109 35.5519 37.0 37.0 37.0 37.0 37.0 110-114 35.4419 37.0 37.0 37.0 37.0 37.0 115-119 35.3508 37.0 37.0 37.0 34.6 37.0 120-124 35.450300000000006 37.0 37.0 37.0 37.0 37.0 125-129 35.189299999999996 37.0 37.0 37.0 29.8 37.0 130-134 35.0932 37.0 37.0 37.0 27.4 37.0 135-139 35.121300000000005 37.0 37.0 37.0 27.4 37.0 140-144 34.9222 37.0 37.0 37.0 25.0 37.0 145-149 34.9788 37.0 37.0 37.0 25.0 37.0 150-151 34.719 37.0 37.0 37.0 25.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.0 1101 2 0.0 1101 3 0.0 1101 4 0.0 1101 5 0.0 1101 6 0.0 1101 7 0.0 1101 8 0.0 1101 9 0.0 1101 10-14 0.0 1101 15-19 0.0 1101 20-24 0.0 1101 25-29 0.0 1101 30-34 0.0 1101 35-39 0.0 1101 40-44 0.0 1101 45-49 0.0 1101 50-54 0.0 1101 55-59 0.0 1101 60-64 0.0 1101 65-69 0.0 1101 70-74 0.0 1101 75-79 0.0 1101 80-84 0.0 1101 85-89 0.0 1101 90-94 0.0 1101 95-99 0.0 1101 100-104 0.0 1101 105-109 0.0 1101 110-114 0.0 1101 115-119 0.0 1101 120-124 0.0 1101 125-129 0.0 1101 130-134 0.0 1101 135-139 0.0 1101 140-144 0.0 1101 145-149 0.0 1101 150-151 0.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 20 6.0 21 7.0 22 6.0 23 6.0 24 6.0 25 14.0 26 8.0 27 19.0 28 23.0 29 35.0 30 34.0 31 57.0 32 57.0 33 92.0 34 154.0 35 451.0 36 2815.0 37 210.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 48.77254509018036 12.850701402805612 6.487975951903807 31.88877755511022 2 21.075 12.950000000000001 32.65 33.324999999999996 3 19.125 19.875 31.674999999999997 29.325000000000003 4 22.75 25.124999999999996 25.825 26.3 5 25.525 33.5 23.1 17.875 6 22.05 33.800000000000004 23.925 20.225 7 17.1 25.55 41.525 15.825 8 17.525 25.874999999999996 31.15 25.45 9 19.35 22.025 34.25 24.375 10-14 20.808121218182727 28.644296644496674 27.974196129419415 22.573386007901185 15-19 20.075000000000003 27.575 27.415 24.935 20-24 20.275000000000002 28.144999999999996 27.355 24.224999999999998 25-29 20.205000000000002 28.249999999999996 27.705000000000002 23.84 30-34 20.8 27.375 27.495000000000005 24.33 35-39 20.064999999999998 27.865000000000002 27.994999999999997 24.075 40-44 20.605 27.775 27.48 24.14 45-49 20.73 27.83 27.665 23.775 50-54 20.65 28.29 27.334999999999997 23.724999999999998 55-59 20.755000000000003 27.76 27.875 23.61 60-64 21.52 27.01 26.889999999999997 24.58 65-69 19.855 28.294999999999998 27.275 24.575 70-74 21.355 27.58 27.505000000000003 23.56 75-79 20.880000000000003 27.935 26.979999999999997 24.205 80-84 21.65 27.065 27.21 24.075 85-89 21.695 27.345000000000002 27.065 23.895 90-94 21.7 28.215 26.669999999999998 23.415 95-99 21.92 27.245 26.765 24.07 100-104 21.945 27.139999999999997 26.979999999999997 23.935000000000002 105-109 20.71 27.61 27.560000000000002 24.12 110-114 21.55 27.48 26.76 24.21 115-119 22.06 28.21 26.155 23.575 120-124 22.29 26.865 27.395000000000003 23.45 125-129 22.145 27.495000000000005 25.83 24.529999999999998 130-134 21.545 28.444999999999997 25.945 24.065 135-139 22.665 26.979999999999997 25.69 24.665 140-144 22.11 27.310000000000002 26.105 24.474999999999998 145-149 22.264999999999997 27.785 26.05 23.9 150-151 21.875 27.187499999999996 25.2375 25.7 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 0.0 1 0.0 2 0.5 3 0.5 4 1.5 5 1.5 6 1.0 7 1.5 8 1.0 9 1.0 10 0.5 11 0.0 12 0.0 13 0.0 14 0.5 15 1.0 16 0.5 17 0.0 18 2.0 19 2.5 20 0.5 21 0.0 22 5.0 23 5.0 24 0.5 25 6.5 26 6.0 27 4.5 28 9.0 29 14.5 30 17.5 31 21.5 32 23.0 33 44.0 34 65.0 35 58.0 36 73.0 37 89.5 38 94.0 39 116.5 40 164.0 41 184.5 42 190.5 43 212.0 44 235.0 45 259.5 46 284.5 47 279.5 48 235.5 49 209.5 50 192.0 51 161.0 52 131.5 53 106.5 54 88.5 55 78.0 56 73.0 57 55.5 58 32.5 59 26.5 60 21.0 61 17.0 62 17.0 63 13.0 64 10.0 65 11.0 66 12.5 67 11.5 68 8.0 69 4.5 70 2.5 71 2.0 72 0.5 73 0.0 74 0.5 75 0.5 76 0.0 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.015 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150-151 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 61.650000000000006 #Duplication Level Percentage of deduplicated Percentage of total 1 63.74695863746959 39.300000000000004 2 21.330089213300894 26.3 3 8.718572587185726 16.125 4 3.4874290348742907 8.6 5 1.7437145174371453 5.375 6 0.48661800486618007 1.7999999999999998 7 0.24330900243309003 1.05 8 0.12165450121654502 0.6 9 0.04055150040551501 0.22499999999999998 >10 0.08110300081103002 0.625 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAATCGACATCTCGTAT 15 0.375 TruSeq Adapter, Index 2 (97% over 37bp) GAACACGAGAGATCCCGTAACCAAACAGAACCACAACTAAAACTAAGCCA 10 0.25 No Hit GCAGGCTGGTCTATACTCCGGAAAGAGTCGACCGGATTTATAACGAACAC 9 0.22499999999999998 No Hit CAGAAAGCTGTCTTTAGCCTACTTCCTGGGAGCTGCAGAGCAGGTGGGGT 8 0.2 No Hit CTTCACAGCTTCAATAGCTTCCTCTGCCCTGTCTTGCTGTTTCATAACCA 8 0.2 No Hit GTCTTCACGTCCCCTTGTGGGGGAGGAGCCCATTTCTGGACTTTGCGATC 8 0.2 No Hit GTGAAACATATCTTCAGCTACAGGACCTGCACTGCAAGAAGTAGGTGGAT 7 0.17500000000000002 No Hit GGCACGACAAGGAGGGCACCATTGAGCTGAGAAGTAAAGAAGAATGTTCT 7 0.17500000000000002 No Hit GCTTCTTCCAATGGGGGCATTTCAGCATCGGCTTCAACAGCATCCTCATC 7 0.17500000000000002 No Hit GGCACTTTGGAAGCAACAGGTTCCACTTGGGTAAAAAAATGGTCCCACAA 7 0.17500000000000002 No Hit CTTGCATCATCCAGAGTGATCTTCCATATCCCTGCATATTAAAAGCAACC 7 0.17500000000000002 No Hit CTCCTTTCTCACCTCCTCAACAGCAACATTGTGATCAGTTGATAACCTTT 7 0.17500000000000002 No Hit GTACCACCAAAACAGCATACAAAGCTTCCTTTTCTCCTCTTTTGATATCT 6 0.15 No Hit CGGCGTGGCAATAAAAAGAACATGACATCAACTTCATATACAGCTCTACG 6 0.15 No Hit CCACATCTTTGTTAAACATAACCCCTTTAAGAACCTTCGAATCTTCCAAC 6 0.15 No Hit GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAATCGACATCGCGTAT 6 0.15 TruSeq Adapter, Index 2 (97% over 37bp) CTAGTATGGCAGGTTTGTCAGTTTTGGCTGGTCTTCCAGGTTCCAAAACA 6 0.15 No Hit CCACAAAGTTTGGTCAAAGGCTACTTTGGCAGGAACCACCCACCAATCTT 6 0.15 No Hit CCTTAATTTTGCTCCTGCATATACTGCTGCAGATCCCAGCTCTTCCTCTA 6 0.15 No Hit AGGTGCTCAAGACCTCGAAGGTAGTGCCCCAAAAGCATACACCAGAAAAG 6 0.15 No Hit ATCCTCCCTCTCGGGGCAGTGACGGAATAGCTTAAGGAACCTTTCCCATC 6 0.15 No Hit GTCAATGCCAGCACTTCTTAAGCACAAGTTGTTGCAACTAGAGACCTTCA 6 0.15 No Hit GAGATTTTGCCATGCATTGCAGCCAACATAAGCGGAGTCTGCTTGTGACG 6 0.15 No Hit GGTTGCTGGCAGATGTATAAACATGATAATCATAGTAATCAGCTGGGTGA 6 0.15 No Hit ATTGGAAAAGAAGAGGGCGAATGGATCATCAAGAGTTGGTTTCTCATTTT 5 0.125 No Hit GGCCAGGAGGGTGCAGTCTCAGAGTTTCTTTTATTACCATCCGGAGATAC 5 0.125 No Hit CTTTCTTACATAAAATGTTCTCCTATCATATTACCATGTTAATAAAGATA 5 0.125 No Hit GTTTTCTTGAAGGCAAATGCAGGCATCTGAGACAGTGTTGGATAAATCTT 5 0.125 No Hit GTCCGTGCCAACACAGTATTGAAAAACAAAAGCGCAATACAAGATTTATC 5 0.125 No Hit CAGAAAATTAAGACATGGAGGTAGCACTCTTGATTATATACTATAACTTG 5 0.125 No Hit CAATAATCAAATCAAGCCAGGATCCGCTAAACATGTCACAGCACAAGCCG 5 0.125 No Hit CAACAATTCAGCAAGTCATAAGTATTATCAACCAGTGCCTATCTAAGCAT 5 0.125 No Hit CCAGCATATTGGCAAGCCTAAGCTGCTTCTCCAAAGGCAAATACTCTTTT 5 0.125 No Hit ATCTTGTACAGAATCCAGACCAATGTCTGCCACACCCTGACTCAACTGTT 5 0.125 No Hit GTAAAGATCATCAAAACCCTGAAATATCCCATATTCCGTGGAGTGATTAC 5 0.125 No Hit GTTGAGGTTGGGATTTCCTTTACCCTTTCCCGCGTCTTAGAAGAGGAACC 5 0.125 No Hit ACTTGGTTCTTGGCATTCTGATGGAACGATCTTGCTGAAATCATCTTTCT 5 0.125 No Hit CCTCACAACACGGGAGTGAATAGCACAAGAGACACAGTACTGCATCTTGA 5 0.125 No Hit GTGAGAACCTCTTTCCTTTGGGCTTGAAAATCTATCTCCAATTTGATTCA 5 0.125 No Hit CCTCTCAATATTTTCTGGAGTAATGAAATGACTGGAATCAACGGTCTCAA 5 0.125 No Hit CCGCATTCGATTACAAACATTTCATACATGGAAACACAATCTATATGAAA 5 0.125 No Hit GTCGGAAGAACACTAGCCCCTTCGCATACTAGCCCATGAAACTCCTGGTT 5 0.125 No Hit CCTGTAGCTGCCTGGCTGCAGACCACGACCGCGACACGCTCCACGACAAC 5 0.125 No Hit CGGCAAGATTCTGAAGTATTTCACCCTTTGTTTCACCACCTTCAATCTCA 5 0.125 No Hit GCCAAGTGGAAACATAAAGGGATGAAAGGAGTAAATGGGCTAAGCATAAA 5 0.125 No Hit GCTCAGTTTCAACATGGGGAACATCTATACGAGTTTCAACCTTGTCCTTC 5 0.125 No Hit GTTGCTGTTCGCCCAGTGGGCTCCGCGCGTGAGCACTTCCCACTGATCCA 5 0.125 No Hit GTCGTCGAGTGTATCTTTCTCACGAACTGGATTCTTCTCTTCTGCTTGAA 5 0.125 No Hit GCGCTGATCCAGTTCTCGGAGACTTTATGGTGGATCTTGCCGGGCAGATC 5 0.125 No Hit CACAGTTCTAGAGTGTACTCTAACCATGCATGGAAAGTACGATAATTATG 5 0.125 No Hit CAGAGCCCTCCAGTGCTCGACACCAGCGGTCAGCCTCTTGAAACCGGTGT 5 0.125 No Hit GACTGAGGATGGTACCATTTGTGATCATACCAAGTAGGGAGCCCAAAACA 5 0.125 No Hit GTGGCTGTTGACTTGTTGCTATCTTTAACAGGACTACTCTGCCTTGTAAA 5 0.125 No Hit GCTCGTTAGTTTTCCACAAATGTCAAACACATCATACTTGCAAGCATGTT 5 0.125 No Hit CCGCATTCGGTCCAACCTGTCTAGATGAATCCCCTTGGTTGCCATTAGCA 5 0.125 No Hit CCTGGTTTGAAGAACACAGAGAGTCCAAAATCGATAGCTTTGAGTGAGAA 5 0.125 No Hit GGGAGCCGAACATCAAGTTGAAGCCGCTGTTGTTTTGGTTGGTCTCGAAC 5 0.125 No Hit GACTTTTACTTCCTCTAAATGACCGAGTTTGACGAACTTTCCGGCTCGGG 5 0.125 No Hit GCACATCAAAGTTTGACTCATCTTCAGCTCCTTCAACATCATACTCACTG 5 0.125 No Hit GGGGGATGAGCGGCAGGTTTTGGTTTAGGTTGTCTAGGCTTCTTTTCTCT 5 0.125 No Hit GCATCCTTGAGGAAAAGAAGCAAGTGACTTGCCACAAGCGCCCTTAAGCA 5 0.125 No Hit ACGCAACTCAGCAGCAGAAATGAAGCCATTCTGGTCCTTATCAAAAACTC 5 0.125 No Hit CTTTTTTTATAATAAAAAATAAAAAATAAAATGTGTAACAAAAGAAGAAA 5 0.125 No Hit ATTATTGCAGGATGCTATAGTGGCCAACCCAGCTGATACCATACTCTTCA 5 0.125 No Hit AGCTCACAAGCCAATGGCAACACAAAAGAAAAGGTGCCAGTTCTTGACAA 5 0.125 No Hit ATAGTGGTAGTCCTCAATGGTCTGATCAATTTCTGCCTGTGTGTTCATCA 5 0.125 No Hit GTGCAGTTTGATCCACACTTGCAGCCATTCTCAGCACCGAAGTCCATCTC 5 0.125 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0 0.0 0.0 0.0 0.0 64-65 0.0 0.0 0.0 0.0 0.0 66-67 0.0 0.0 0.0 0.0 0.0 68-69 0.0 0.0 0.0 0.0 0.0 70-71 0.025 0.0 0.0 0.0 0.0 72-73 0.025 0.0 0.0 0.0 0.0 74-75 0.025 0.0 0.0 0.0 0.0 76-77 0.025 0.0 0.0 0.0 0.0 78-79 0.025 0.0 0.0 0.0 0.0 80-81 0.037500000000000006 0.0 0.0 0.0 0.0 82-83 0.05 0.0 0.0 0.0 0.0 84-85 0.1 0.0 0.0 0.0 0.0 86-87 0.1625 0.0 0.0 0.0 0.0 88-89 0.225 0.0 0.0 0.0 0.0 90-91 0.32499999999999996 0.0 0.0 0.0 0.0 92-93 0.5 0.0 0.0 0.0 0.0 94-95 0.7250000000000001 0.0 0.0 0.0 0.0 96-97 0.8875 0.0 0.0 0.0 0.0 98-99 1.075 0.0 0.0 0.0 0.0 100-101 1.1375 0.0 0.0 0.0 0.0 102-103 1.3875000000000002 0.0 0.0 0.0 0.0 104-105 1.775 0.0 0.0 0.0 0.0 106-107 2.1500000000000004 0.0 0.0 0.0 0.0 108-109 2.3875 0.0 0.0 0.0 0.0 110-111 2.5625 0.0 0.0 0.0 0.0 112-113 2.6375 0.0 0.0 0.0 0.0 114-115 3.0625 0.0 0.0 0.0 0.0 116-117 3.5125 0.0 0.0 0.0 0.0 118-119 3.975 0.0 0.0 0.0 0.0 120-121 4.525 0.0 0.0 0.0 0.0 122-123 5.1375 0.0 0.0 0.0 0.0 124-125 5.8125 0.0 0.0 0.0 0.0 126-127 6.4 0.0 0.0 0.0 0.0 128-129 7.0625 0.0 0.0 0.0 0.0 130-131 7.7875 0.0 0.0 0.0 0.0 132-133 8.475 0.0 0.0 0.0 0.0 134-135 8.9875 0.0 0.0 0.0 0.0 136-137 9.65 0.0 0.0 0.0 0.0 138-139 10.0625 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content pass >>END_MODULE SRR26075370 read2 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR26075370_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.28075 37.0 37.0 37.0 37.0 37.0 2 36.1195 37.0 37.0 37.0 37.0 37.0 3 36.1685 37.0 37.0 37.0 37.0 37.0 4 36.1335 37.0 37.0 37.0 37.0 37.0 5 36.0905 37.0 37.0 37.0 37.0 37.0 6 35.917 37.0 37.0 37.0 37.0 37.0 7 35.8895 37.0 37.0 37.0 37.0 37.0 8 35.903 37.0 37.0 37.0 37.0 37.0 9 35.9585 37.0 37.0 37.0 37.0 37.0 10-14 35.8803 37.0 37.0 37.0 37.0 37.0 15-19 35.7462 37.0 37.0 37.0 37.0 37.0 20-24 35.693200000000004 37.0 37.0 37.0 37.0 37.0 25-29 35.5637 37.0 37.0 37.0 37.0 37.0 30-34 35.352599999999995 37.0 37.0 37.0 37.0 37.0 35-39 35.3274 37.0 37.0 37.0 37.0 37.0 40-44 35.2207 37.0 37.0 37.0 37.0 37.0 45-49 35.155199999999994 37.0 37.0 37.0 37.0 37.0 50-54 34.9322 37.0 37.0 37.0 32.2 37.0 55-59 34.988600000000005 37.0 37.0 37.0 32.2 37.0 60-64 35.0632 37.0 37.0 37.0 37.0 37.0 65-69 34.9777 37.0 37.0 37.0 29.8 37.0 70-74 34.8422 37.0 37.0 37.0 27.4 37.0 75-79 34.783100000000005 37.0 37.0 37.0 25.0 37.0 80-84 34.8914 37.0 37.0 37.0 25.0 37.0 85-89 34.811099999999996 37.0 37.0 37.0 25.0 37.0 90-94 34.6862 37.0 37.0 37.0 25.0 37.0 95-99 34.77910000000001 37.0 37.0 37.0 25.0 37.0 100-104 34.6899 37.0 37.0 37.0 25.0 37.0 105-109 34.6839 37.0 37.0 37.0 25.0 37.0 110-114 34.5818 37.0 37.0 37.0 25.0 37.0 115-119 34.5904 37.0 37.0 37.0 25.0 37.0 120-124 34.365249999999996 37.0 37.0 37.0 25.0 37.0 125-129 34.43390000000001 37.0 37.0 37.0 25.0 37.0 130-134 34.3619 37.0 37.0 37.0 25.0 37.0 135-139 34.24405 37.0 37.0 37.0 25.0 37.0 140-144 34.255950000000006 37.0 37.0 37.0 25.0 37.0 145-149 34.173249999999996 37.0 37.0 37.0 25.0 37.0 150-151 34.061375 37.0 37.0 37.0 25.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.0 1101 2 0.0 1101 3 0.0 1101 4 0.0 1101 5 0.0 1101 6 0.0 1101 7 0.0 1101 8 0.0 1101 9 0.0 1101 10-14 0.0 1101 15-19 0.0 1101 20-24 0.0 1101 25-29 0.0 1101 30-34 0.0 1101 35-39 0.0 1101 40-44 0.0 1101 45-49 0.0 1101 50-54 0.0 1101 55-59 0.0 1101 60-64 0.0 1101 65-69 0.0 1101 70-74 0.0 1101 75-79 0.0 1101 80-84 0.0 1101 85-89 0.0 1101 90-94 0.0 1101 95-99 0.0 1101 100-104 0.0 1101 105-109 0.0 1101 110-114 0.0 1101 115-119 0.0 1101 120-124 0.0 1101 125-129 0.0 1101 130-134 0.0 1101 135-139 0.0 1101 140-144 0.0 1101 145-149 0.0 1101 150-151 0.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 12 2.0 13 11.0 14 19.0 15 25.0 16 19.0 17 23.0 18 10.0 19 19.0 20 18.0 21 17.0 22 21.0 23 27.0 24 20.0 25 23.0 26 16.0 27 20.0 28 22.0 29 25.0 30 25.0 31 35.0 32 40.0 33 67.0 34 180.0 35 577.0 36 2546.0 37 193.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 53.013253313328335 19.779944986246562 8.727181795448862 18.479619904976243 2 31.825 22.925 25.75 19.5 3 26.400000000000002 27.175 29.599999999999998 16.825000000000003 4 28.449999999999996 32.125 21.875 17.549999999999997 5 28.925 34.075 20.075000000000003 16.925 6 27.825 36.0 20.325 15.85 7 25.074999999999996 21.825 33.475 19.625 8 24.7 23.625 26.375 25.3 9 26.575 24.55 26.450000000000003 22.425 10-14 26.775 28.835 23.82 20.57 15-19 27.355 26.979999999999997 25.56 20.105 20-24 26.685 27.99 24.075 21.25 25-29 26.640000000000004 27.295 25.724999999999998 20.34 30-34 26.55 28.165000000000003 24.785 20.5 35-39 26.895000000000003 28.235 24.86 20.01 40-44 26.810000000000002 27.389999999999997 25.295 20.505000000000003 45-49 26.76 26.640000000000004 26.279999999999998 20.32 50-54 24.26 27.015 27.810000000000002 20.915 55-59 25.974999999999998 27.725 25.385 20.915 60-64 26.38 27.66 25.6 20.36 65-69 25.495 28.21 25.314999999999998 20.979999999999997 70-74 24.255 29.854999999999997 25.369999999999997 20.52 75-79 24.385 29.14 25.935000000000002 20.54 80-84 24.86 28.955 25.135 21.05 85-89 25.729999999999997 28.7 25.080000000000002 20.49 90-94 26.784999999999997 28.415000000000003 25.21 19.59 95-99 25.715 28.185 26.265 19.835 100-104 26.119999999999997 28.15 26.27 19.46 105-109 24.995 28.444999999999997 26.029999999999998 20.53 110-114 26.235000000000003 28.005000000000003 25.31 20.45 115-119 25.4 29.104999999999997 25.874999999999996 19.62 120-124 25.681284064203208 28.86144307215361 25.92129606480324 19.53597679883994 125-129 26.63 27.744999999999997 26.395000000000003 19.23 130-134 26.169999999999998 28.53 25.75 19.55 135-139 27.394109116367453 27.499124868730306 26.273941091163678 18.83282492373856 140-144 26.31394709206381 28.18422763414512 25.648847327099066 19.852977946692004 145-149 27.836959239809957 28.787196799199798 24.646161540385098 18.72968242060515 150-151 28.103512939117394 27.090886360795096 26.440805100637583 18.36479559944993 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 1.0 6 1.0 7 3.0 8 3.5 9 1.0 10 1.5 11 2.5 12 3.5 13 2.5 14 2.0 15 2.0 16 2.5 17 4.0 18 4.0 19 3.5 20 4.5 21 5.0 22 3.0 23 3.0 24 3.0 25 3.5 26 4.5 27 5.0 28 5.5 29 6.5 30 10.0 31 13.0 32 18.0 33 29.5 34 33.5 35 42.0 36 56.0 37 74.5 38 105.5 39 129.0 40 165.0 41 196.0 42 215.0 43 244.0 44 268.0 45 280.0 46 252.5 47 210.0 48 198.0 49 199.0 50 173.5 51 146.5 52 140.0 53 112.5 54 86.5 55 74.5 56 49.0 57 46.5 58 51.0 59 37.0 60 24.5 61 14.5 62 15.5 63 14.0 64 10.0 65 6.0 66 5.5 67 7.0 68 6.0 69 6.5 70 3.0 71 0.5 72 0.5 73 1.0 74 3.0 75 3.0 76 3.0 77 4.0 78 3.5 79 3.5 80 4.5 81 5.5 82 6.0 83 5.0 84 3.5 85 4.0 86 3.5 87 3.5 88 5.0 89 3.5 90 3.5 91 6.0 92 6.0 93 5.0 94 5.5 95 5.0 96 3.0 97 2.0 98 2.0 99 2.0 100 23.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.025 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.005 125-129 0.0 130-134 0.0 135-139 0.015 140-144 0.015 145-149 0.025 150-151 0.0125 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 62.375 #Duplication Level Percentage of deduplicated Percentage of total 1 66.65330661322645 41.575 2 19.639278557114228 24.5 3 8.176352705410823 15.299999999999999 4 3.0460921843687374 7.6 5 1.56312625250501 4.875 6 0.4008016032064128 1.5 7 0.24048096192384769 1.05 8 0.12024048096192384 0.6 9 0.04008016032064128 0.22499999999999998 >10 0.08016032064128256 0.9249999999999999 >50 0.04008016032064128 1.8499999999999999 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG 74 1.8499999999999999 No Hit GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGT 27 0.675 No Hit GGTGGTGGACGTGACCGTGGGTACGGTGATGGTGGATCAAGGTATTCTTC 10 0.25 No Hit GTCGAACCAAACCGAGACACCGTCATCCTCAGCATCCTCAACCTCGTCAA 9 0.22499999999999998 No Hit GGATCATCATCTCTGTCGTTGCCTGGTACATCAACACCACCAATGTCCCC 8 0.2 No Hit CCGTCAAAGAAAAGAATCAGAAGCTCCATATCATCATCTAGTTGAGAAGG 8 0.2 No Hit GAGGTATCGACCCCTCTTGTGCCGCAGCTAACGCATTAAGTATCCCGCCT 8 0.2 No Hit GGTTTACCCAGCGGAATATACTCAGGGTGTATCCAAAGGGTATTCGTGTG 7 0.17500000000000002 No Hit AAGACTTTGAACCCAAATGTAGCTGAACTAATCGAAGAACATGGTATCGA 7 0.17500000000000002 No Hit AAAAACCAAAAGCAGACCCTCCTCCTTCTCCGCGGCTACCTCTTTGTCTG 7 0.17500000000000002 No Hit GAGAGAATCATGAGGGCTCAAGCCTTGAGAGACAACAGCATGGCTGGGTA 7 0.17500000000000002 No Hit AAGCAGCTGATCCTGAAGAGCAATTCGCTAGCCTGTTAAAGTTAATTAAT 7 0.17500000000000002 No Hit GTTTGGGGACATGGGAACTTCAACACCATATGCTACCTTTATCCGTACAC 7 0.17500000000000002 No Hit CTAAAGACAAACCCAGTTTTAGCAAAAATGATGATTAGTGGCATAGTCTA 6 0.15 No Hit GCAGGGACCAAAGCTTGCCGCGAGAAGTTGCGGAGGGAGAGATTGAATGA 6 0.15 No Hit GCTAGATACTAGTAGTAGTTGCAGACAGAGAGCTAGTGTTAGACAGGTTC 6 0.15 No Hit TGCATGGTTATAGAGAGACCTCTACTGGTCATCGTGATCTCTTAACAATG 6 0.15 No Hit CCTGTCTGGAACGAGAAGAAGCGCACTGCAACAGGATTTGGCATCCAAAC 6 0.15 No Hit ACAATACCTTGATCGTTTTTGCTGTTTGTAGAGGAAAATAGTATCATGGA 6 0.15 No Hit GCACAGTCTTTCCTTCCCTCCCATTAGCTATTAAATGGCTTAGGGAAAGT 6 0.15 No Hit CCCACATCCCCTGAATTAAAAGAAACCATCCATTCTGTGGTCCATGGCCT 6 0.15 No Hit CGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGGCTAGACTAGCGT 6 0.15 No Hit TGAAGGTGAATCAAATTGGTTCTGTAACTGAAAGTATTGAGGCTGTGAAA 6 0.15 No Hit GCTCTATCTTCATCAAATTCATTCTAATTCAATTTTATTTATCAGTATTT 5 0.125 No Hit TGAAACCACGAACGGTCATCCTGGACGAGAAGCGGAAGGATATGTTCAGA 5 0.125 No Hit GTCATCAGCACGCGTTGATTACGTCCCTGCCCTTTGTACACACCGCCCGT 5 0.125 No Hit CAGAAGGTCACAATGTTTTCCAGCCAAAAATCCAACACCAGCAGGACAAC 5 0.125 No Hit GGTTGGAGAACTAGATGTTTGTATGGGAGCTCATGTGGTTCTTCGAAGTG 5 0.125 No Hit CGCAAGCTTGTGAGGCAATCCAAGTGCTTGCAGGACAAGATGACAGCCAA 5 0.125 No Hit GTAAAATTAAAATATCGCCAGAAGAGGATGATTTTTACCTTTACTGTGTT 5 0.125 No Hit CCATGACATTCAAGCGAAGGAATGGAGGCCGCAACAAGCACGGCCGTGGA 5 0.125 No Hit GTGTTGGGTTGCTCCTGGTCAGGTCCCAGCAACTGGAAATGTGAAGCCTG 5 0.125 No Hit GTGGAATTAACTCTACAGCAGTCAAGGTCAGCAGGGCCATTAACAAGTTG 5 0.125 No Hit TCAATTGCGGTTGTTCAAGCTAATTCTAATTTAGAAGATCAAAGTCAGGT 5 0.125 No Hit CTTTCTCCAGCTGAGATTCAAGAAGCTGCAGCAAGGTTTGCCAATTCGGA 5 0.125 No Hit GCTTCACATGTTATCACTTCACCTTCCCTCCTTAGCTTACACTGTTCCAG 5 0.125 No Hit GAGAGTTTTTACCAGCATGCAAGAGAAGGCACCAACAAGTGTTGTTTTGG 5 0.125 No Hit GTTCTTATTAGCGAGGGTTGTTTAGGAGAAAAACCGGTGCCTTTATTTTT 5 0.125 No Hit CATAGATGCAGGCCCAGATGGACAGGACTTGGTTTATCGTGTGGAGAAGA 5 0.125 No Hit AACAATGGAAAGCGTCTCCTACCAACGCTTCCCAAAAATCAAAATCCGAG 5 0.125 No Hit ACCATCTTCACTTCCAGGCTTACTACTTGACTGTGCCTTTTCCGATTGAG 5 0.125 No Hit GAGAAGGATTCGACTGCATCTACTTTGGCTCAAAGAAATCGGTCTTTTGG 5 0.125 No Hit AGAACAGAGAATCTGCAGCTCGGTCCCGGGCTAGGAAGCAGGCTTACACG 5 0.125 No Hit CATCAACCGAACGCATATACTACTTGGTTACTGACAAAGGACCCCCTGAC 5 0.125 No Hit GTTCAGAGAAGGACTCCTCCCCAAATTCGTTCAAAATCTCCAATTAGATC 5 0.125 No Hit GCAAAGATTGTTAGAGTTTGAAACAAGAAAAAGAACGTGTTGTTCGTGCT 5 0.125 No Hit GTTTTTTGCCAGCAGGAATTTAGTGCCCTGTGCGTTTAACAAGTCATCAA 5 0.125 No Hit CCTTCTCTCCAGTCTCCAAACAAAAATGGCCGATCAGCTCACCGAAGACC 5 0.125 No Hit GTGAAACTGAAGGCGGTGGTGTATGCTCTGTCACCGTTTCAGCAGAAGGT 5 0.125 No Hit CTTTCATCTACAACACCCAGCAACAATGACCAAAGCGACACTCCAATCCA 5 0.125 No Hit GTGAAGATGACTGAAGTGCCATTTGGTTCAGCACTTGCCAGGGGTTCCTG 5 0.125 No Hit GGGGAGGTTTTAGTATAATTGGGTATCAATGGCGGACACTTCATTTTAAT 5 0.125 No Hit CCAATCTTCTTCTCATAGCAGTCCACTATGGTTGAATGCTTTGCTATCAG 5 0.125 No Hit CTCAAATGCTCTCTTTATTCCCGGTGAACTTGAGTTGCCGTTTATGGCGT 5 0.125 No Hit TGAGATGGAAGCTTGTATCATGGATGGAAATTCAAAGAAATGTGGAGCTG 5 0.125 No Hit GCCCTTCAAGATGTAATGAAGAACCCAGCTAATCTTGCTAAGCATCAAGG 5 0.125 No Hit GGAGCAACCAACTGCTACGGAGGAGCCTAAGGTGGAGGAGAATCCCGTTA 5 0.125 No Hit TCCAGAATCATGGAATGGGTTTGTGTATATTATTGAAGGTGAAGGGGTCT 5 0.125 No Hit CAATCCTCCTGGAGTAGACACTATTTCACTGACAGTGAATTGGGCAATGG 5 0.125 No Hit ATGTTGCTGTTGGGAATGAGGATTGTGGGAAGAAGAATTACCGAGGAAAT 5 0.125 No Hit AGAAAAAGAAAAAAAGAAGGGATCGATGGATATATGTGGGTTGTGGAGTG 5 0.125 No Hit CCCGGTCAAGCCACCACCTGGCCCGATGAAGCCACCACGTGGCCCGGTCA 5 0.125 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0 0.0 0.0 0.0 0.0 64-65 0.0 0.0 0.0 0.0 0.0 66-67 0.0 0.0 0.0 0.0 0.0 68-69 0.0 0.0 0.0 0.0 0.0 70-71 0.025 0.0 0.0 0.0 0.0 72-73 0.025 0.0 0.0 0.0 0.0 74-75 0.037500000000000006 0.0 0.0 0.0 0.0 76-77 0.05 0.0 0.0 0.0 0.0 78-79 0.05 0.0 0.0 0.0 0.0 80-81 0.0625 0.0 0.0 0.0 0.0 82-83 0.075 0.0 0.0 0.0 0.0 84-85 0.125 0.0 0.0 0.0 0.0 86-87 0.1875 0.0 0.0 0.0 0.0 88-89 0.25 0.0 0.0 0.0 0.0 90-91 0.35 0.0 0.0 0.0 0.0 92-93 0.5375 0.0 0.0 0.0 0.0 94-95 0.7749999999999999 0.0 0.0 0.0 0.0 96-97 0.925 0.0 0.0 0.0 0.0 98-99 1.1 0.0 0.0 0.0 0.0 100-101 1.1625 0.0 0.0 0.0 0.0 102-103 1.4 0.0 0.0 0.0 0.0 104-105 1.775 0.0 0.0 0.0 0.0 106-107 2.1500000000000004 0.0 0.0 0.0 0.0 108-109 2.3875 0.0 0.0 0.0 0.0 110-111 2.5625 0.0 0.0 0.0 0.0 112-113 2.6375 0.0 0.0 0.0 0.0 114-115 3.0625 0.0 0.0 0.0 0.0 116-117 3.5 0.0 0.0 0.0 0.0 118-119 3.95 0.0 0.0 0.0 0.0 120-121 4.55 0.0 0.0 0.0 0.0 122-123 5.225 0.0 0.0 0.0 0.0 124-125 5.95 0.0 0.0 0.0 0.0 126-127 6.6375 0.0 0.0 0.0 0.0 128-129 7.325 0.0 0.0 0.0 0.0 130-131 8.0875 0.0 0.0 0.0 0.0 132-133 8.775 0.0 0.0 0.0 0.0 134-135 9.287500000000001 0.0 0.0 0.0 0.0 136-137 9.962499999999999 0.0 0.0 0.0 0.0 138-139 10.3875 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AACATGA 10 0.006830828 145.0 5 >>END_MODULE Read 1561336 spots for SRR26075370.sra Written 1561336 spots for SRR26075370.sra Read 1561336 spots for SRR26075370.sra Written 1561336 spots for SRR26075370.sra Read 1561336 spots for SRR26075370.sra Written 1561336 spots for SRR26075370.sra Read 1561336 spots for SRR26075370.sra Written 1561336 spots for SRR26075370.sra Read 1561336 spots for SRR26075370.sra Written 1561336 spots for SRR26075370.sra Read 1561336 spots for SRR26075370.sra Written 1561336 spots for SRR26075370.sra Read 1561336 spots for SRR26075370.sra Written 1561336 spots for SRR26075370.sra Read 1561336 spots for SRR26075370.sra Written 1561336 spots for SRR26075370.sra Read 1561336 spots for SRR26075370.sra Written 1561336 spots for SRR26075370.sra Read 1561336 spots for SRR26075370.sra Written 1561336 spots for SRR26075370.sra Read 1561336 spots for SRR26075370.sra Written 1561336 spots for SRR26075370.sra Read 1561336 spots for SRR26075370.sra Written 1561336 spots for SRR26075370.sra Read 1561336 spots for SRR26075370.sra Written 1561336 spots for SRR26075370.sra Read 1561336 spots for SRR26075370.sra Written 1561336 spots for SRR26075370.sra Read 1561336 spots for SRR26075370.sra Written 1561336 spots for SRR26075370.sra Read 1561336 spots for SRR26075370.sra Written 1561336 spots for SRR26075370.sra Read 1561336 spots for SRR26075370.sra Written 1561336 spots for SRR26075370.sra Read 1561336 spots for SRR26075370.sra Written 1561336 spots for SRR26075370.sra Read 1561336 spots for SRR26075370.sra Written 1561336 spots for SRR26075370.sra Read 1561339 spots for SRR26075370.sra Written 1561339 spots for SRR26075370.sra SRR ids: ['SRR26075370.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd__qg25kig SRR26075370.sra spots: 31226723 blocks: [[1, 1561336], [1561337, 3122672], [3122673, 4684008], [4684009, 6245344], [6245345, 7806680], [7806681, 9368016], [9368017, 10929352], [10929353, 12490688], [12490689, 14052024], [14052025, 15613360], [15613361, 17174696], [17174697, 18736032], [18736033, 20297368], [20297369, 21858704], [21858705, 23420040], [23420041, 24981376], [24981377, 26542712], [26542713, 28104048], [28104049, 29665384], [29665385, 31226723]] SRR26075370 file size 11530395 SRR26075370 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o SRR26075370 SRR26075370_1.fastq SRR26075370_2.fastq Input file: SRR26075370_1.fastq Paired file: SRR26075370_2.fastq trimmed: SRR26075370-trimmed-pair1.fastq, SRR26075370-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Tue Feb 11 23:56:17 2025 >> started Tue Feb 11 23:57:07 2025 >> done (50.509s) 31226723 read pairs processed; of these: 225 ( 0.00%) short read pairs filtered out after trimming by size control 110688 ( 0.35%) empty read pairs filtered out after trimming by size control 31115810 (99.64%) read pairs available; of these: 4507664 (14.49%) trimmed read pairs available after processing 26608146 (85.51%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 18 16 0.00% 19 35 0.00% 20 27 0.00% 21 41 0.00% 22 44 0.00% 23 52 0.00% 24 67 0.00% 25 84 0.00% 26 91 0.00% 27 97 0.00% 28 106 0.00% 29 83 0.00% 30 93 0.00% 31 74 0.00% 32 68 0.00% 33 96 0.00% 34 98 0.00% 35 95 0.00% 36 105 0.00% 37 117 0.00% 38 145 0.00% 39 100 0.00% 40 124 0.00% 41 137 0.00% 42 138 0.00% 43 172 0.00% 44 161 0.00% 45 195 0.00% 46 164 0.00% 47 167 0.00% 48 216 0.00% 49 279 0.00% 50 228 0.00% 51 327 0.00% 52 330 0.00% 53 314 0.00% 54 352 0.00% 55 372 0.00% 56 516 0.00% 57 584 0.00% 58 584 0.00% 59 666 0.00% 60 746 0.00% 61 830 0.00% 62 1057 0.00% 63 1023 0.00% 64 1098 0.00% 65 1223 0.00% 66 1368 0.00% 67 1525 0.00% 68 1825 0.01% 69 2131 0.01% 70 2604 0.01% 71 2781 0.01% 72 3241 0.01% 73 3765 0.01% 74 3797 0.01% 75 4459 0.01% 76 4824 0.02% 77 5219 0.02% 78 6000 0.02% 79 6651 0.02% 80 7664 0.02% 81 8461 0.03% 82 9597 0.03% 83 10541 0.03% 84 11840 0.04% 85 12552 0.04% 86 13163 0.04% 87 14240 0.05% 88 14855 0.05% 89 16194 0.05% 90 18173 0.06% 91 19431 0.06% 92 21512 0.07% 93 23930 0.08% 94 24809 0.08% 95 26227 0.08% 96 27812 0.09% 97 28419 0.09% 98 29655 0.10% 99 31482 0.10% 100 32498 0.10% 101 34860 0.11% 102 38333 0.12% 103 39981 0.13% 104 42145 0.14% 105 44100 0.14% 106 45714 0.15% 107 45996 0.15% 108 47497 0.15% 109 48450 0.16% 110 49762 0.16% 111 52486 0.17% 112 56152 0.18% 113 57478 0.18% 114 61071 0.20% 115 63273 0.20% 116 63395 0.20% 117 65710 0.21% 118 65582 0.21% 119 66277 0.21% 120 67793 0.22% 121 70347 0.23% 122 74855 0.24% 123 77934 0.25% 124 81219 0.26% 125 81934 0.26% 126 84297 0.27% 127 85172 0.27% 128 85355 0.27% 129 86675 0.28% 130 87550 0.28% 131 88925 0.29% 132 93746 0.30% 133 95287 0.31% 134 97333 0.31% 135 101143 0.33% 136 102685 0.33% 137 102968 0.33% 138 103783 0.33% 139 105389 0.34% 140 105311 0.34% 141 108176 0.35% 142 109551 0.35% 143 112602 0.36% 144 116511 0.37% 145 118710 0.38% 146 120593 0.39% 147 120448 0.39% 148 121345 0.39% 149 122692 0.39% 150 122091 0.39% 151 26608146 85.51% 31115810 reads passed initial QC criterion=sequence-density sequence-density=0.35 sequence-density-rank=1 fanout-score=25.39 fanout-score-rank=5 prefix-density=0.33 prefix-fanout=25.4 sequence=GGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAATCGACATCTCGTATGCCGTCTTCTGCTTGAAAA criterion=fanout-score sequence-density=0.03 sequence-density-rank=30 fanout-score=388.60 fanout-score-rank=1 prefix-density=0.61 prefix-fanout=17.7 sequence=CCACCACCACCTCCACCACTTCCGCGGGATTGAGCTTCGTTCACGGTGATGTTACGGCCATCGAGGTCCTGACCGTTCATTCCATCAATAGCATCTCTCATTGCCTTCTC criterion=sequence-density sequence-density=0.29 sequence-density-rank=1 fanout-score=2.61 fanout-score-rank=29 prefix-density=0.31 prefix-fanout=2.4 sequence=TGGTTTTACTAG criterion=fanout-score sequence-density=0.01 sequence-density-rank=36 fanout-score=29.27 fanout-score-rank=1 prefix-density=0.19 prefix-fanout=1.7 sequence=TCTTTGAGAGTGCATAGATTTGTGTTGATATAGAAAACAATGGCACTACATGGAAAGATTGAGACAACATTAGAACTCAAGTCCTCCGCAGAGAAGTTCTACAAAGTGTGGAGGAGCCAGTCCTTCCATGTTCCCAAACATGCTTCCAAGCATATCCAAGGAGTTGATATACATGCAGGTGACTGGGAGACTGCGGGCTCTATCAGGATTTGGCAGTACACAATCGGAGGGAAAGCCGGGGTCTTTAAAGAGGAGGTTTCC SRR26075370 testing PE reads STAR mapping to Ensembl genome Started job on | Feb 11 23:57:52 Started mapping on | Feb 11 23:57:52 Finished on | Feb 12 00:05:31 Mapping speed, Million of reads per hour | 244.05 Number of input reads | 31115810 Average input read length | 294 UNIQUE READS: Uniquely mapped reads number | 25405401 Uniquely mapped reads % | 81.65% Average mapped length | 292.72 Number of splices: Total | 22076628 Number of splices: Annotated (sjdb) | 21483379 Number of splices: GT/AG | 21660249 Number of splices: GC/AG | 315546 Number of splices: AT/AC | 22992 Number of splices: Non-canonical | 77841 Mismatch rate per base, % | 0.41% Deletion rate per base | 0.03% Deletion average length | 2.98 Insertion rate per base | 0.02% Insertion average length | 2.55 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 797061 % of reads mapped to multiple loci | 2.56% Number of reads mapped to too many loci | 90751 % of reads mapped to too many loci | 0.29% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 14.39% % of reads unmapped: other | 1.11% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 4913348 4913348 4913348 N_multimapping 797061 797061 797061 N_noFeature 742991 25112841 918931 N_ambiguous 287813 1761 170045 UnstrandedReadsAssigned:24374597 PositiveStrandReadsAssigned:290799 NegativeStrandReadsAssigned:24316425 Dataset is classified negative stranded MeadianReadLen=151 20thPercentileLength=151 echo kmer=147 SRR26075370 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,400 [index] number of k-mers: 62,057,036 [index] number of equivalence classes: 130,681 [quant] running in paired-end mode [quant] will process pair 1: SRR26075370-trimmed-pair1.fastq SRR26075370-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 31,115,810 reads, 25,265,017 reads pseudoaligned [quant] estimated average fragment length: 214.599 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,106 rounds 52401 SRR26075370.ke.tsv 34699 SRR26075370.se.tsv 87100 total ==> SRR26075370.ke.tsv <== target_id length eff_length est_counts tpm Potri.005G200100.1.v4.1 2018 1804.4 2991 58.3958 Potri.005G024800.1.v4.1 1035 821.401 2833 121.504 Potri.004G059700.1.v4.1 961 747.407 6 0.282808 Potri.007G009000.2.v4.1 1416 1202.4 0 0 Potri.003G141000.2.v4.1 2943 2729.4 1117 14.4173 Potri.016G087400.1.v4.1 270 89.0776 1457 576.221 Potri.015G069301.1.v4.1 564 352.786 0 0 Potri.010G195200.1.v4.1 1773 1559.4 770.837 17.4142 Potri.012G127500.1.v4.1 977 763.401 24805 1144.68 ==> SRR26075370.se.tsv <== Potri.001G166300.v4.1 0 Potri.001G448400.v4.1 91 Potri.001G233950.v4.1 0 Potri.001G122700.v4.1 306 Potri.001G212900.v4.1 0 Potri.001G182400.v4.1 0 Potri.001G256600.v4.1 0 Potri.001G040500.v4.1 3 Potri.001G416900.v4.1 0 Potri.001G452600.v4.1 1249 SRR26075370 completed mapping pipeline successfully