Starting /dee2/code/volunteer_pipeline.sh SRR26075371 current disk space = 3048946954240 free memory = 1436976712 SRR26075371 SRAfilesize 0672926435d1eecfc28fae93cad58301 SRR26075371.sra SRR26075371.sra file validated SRR26075371 is paired end SRR26075371 is conventional basespace SRR26075371 read1 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR26075371_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.62225 37.0 37.0 37.0 37.0 37.0 2 36.5365 37.0 37.0 37.0 37.0 37.0 3 36.57 37.0 37.0 37.0 37.0 37.0 4 36.621 37.0 37.0 37.0 37.0 37.0 5 36.6465 37.0 37.0 37.0 37.0 37.0 6 36.6545 37.0 37.0 37.0 37.0 37.0 7 36.6025 37.0 37.0 37.0 37.0 37.0 8 36.6465 37.0 37.0 37.0 37.0 37.0 9 36.6455 37.0 37.0 37.0 37.0 37.0 10-14 36.65220000000001 37.0 37.0 37.0 37.0 37.0 15-19 36.6155 37.0 37.0 37.0 37.0 37.0 20-24 36.6087 37.0 37.0 37.0 37.0 37.0 25-29 36.5083 37.0 37.0 37.0 37.0 37.0 30-34 36.4528 37.0 37.0 37.0 37.0 37.0 35-39 36.4341 37.0 37.0 37.0 37.0 37.0 40-44 36.332499999999996 37.0 37.0 37.0 37.0 37.0 45-49 36.332300000000004 37.0 37.0 37.0 37.0 37.0 50-54 36.333499999999994 37.0 37.0 37.0 37.0 37.0 55-59 36.319900000000004 37.0 37.0 37.0 37.0 37.0 60-64 36.2034 37.0 37.0 37.0 37.0 37.0 65-69 36.1477 37.0 37.0 37.0 37.0 37.0 70-74 36.1112 37.0 37.0 37.0 37.0 37.0 75-79 36.087599999999995 37.0 37.0 37.0 37.0 37.0 80-84 36.06269999999999 37.0 37.0 37.0 37.0 37.0 85-89 35.969100000000005 37.0 37.0 37.0 37.0 37.0 90-94 35.8594 37.0 37.0 37.0 37.0 37.0 95-99 35.882400000000004 37.0 37.0 37.0 37.0 37.0 100-104 35.893299999999996 37.0 37.0 37.0 37.0 37.0 105-109 35.783100000000005 37.0 37.0 37.0 37.0 37.0 110-114 35.6695 37.0 37.0 37.0 37.0 37.0 115-119 35.583299999999994 37.0 37.0 37.0 37.0 37.0 120-124 35.6311 37.0 37.0 37.0 37.0 37.0 125-129 35.4225 37.0 37.0 37.0 37.0 37.0 130-134 35.5 37.0 37.0 37.0 37.0 37.0 135-139 35.277100000000004 37.0 37.0 37.0 29.8 37.0 140-144 35.134699999999995 37.0 37.0 37.0 29.8 37.0 145-149 35.2586 37.0 37.0 37.0 29.8 37.0 150-151 34.947 37.0 37.0 37.0 25.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.0 1101 2 0.0 1101 3 0.0 1101 4 0.0 1101 5 0.0 1101 6 0.0 1101 7 0.0 1101 8 0.0 1101 9 0.0 1101 10-14 0.0 1101 15-19 0.0 1101 20-24 0.0 1101 25-29 0.0 1101 30-34 0.0 1101 35-39 0.0 1101 40-44 0.0 1101 45-49 0.0 1101 50-54 0.0 1101 55-59 0.0 1101 60-64 0.0 1101 65-69 0.0 1101 70-74 0.0 1101 75-79 0.0 1101 80-84 0.0 1101 85-89 0.0 1101 90-94 0.0 1101 95-99 0.0 1101 100-104 0.0 1101 105-109 0.0 1101 110-114 0.0 1101 115-119 0.0 1101 120-124 0.0 1101 125-129 0.0 1101 130-134 0.0 1101 135-139 0.0 1101 140-144 0.0 1101 145-149 0.0 1101 150-151 0.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 23 5.0 24 3.0 25 8.0 26 10.0 27 13.0 28 14.0 29 25.0 30 36.0 31 41.0 32 43.0 33 103.0 34 134.0 35 477.0 36 2908.0 37 180.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.160540135033756 12.678169542385595 7.051762940735183 38.10952738184546 2 19.125 13.8 36.975 30.099999999999998 3 15.15 17.974999999999998 29.475 37.4 4 21.625 25.575 24.725 28.075 5 24.4 31.474999999999998 24.125 20.0 6 20.825 33.300000000000004 23.35 22.525000000000002 7 14.549999999999999 27.425 39.875 18.15 8 15.049999999999999 27.85 32.425 24.675 9 18.8 23.724999999999998 34.575 22.900000000000002 10-14 19.505 29.14 27.6 23.755000000000003 15-19 19.905 27.815 27.97 24.310000000000002 20-24 20.465 26.86 28.59 24.085 25-29 19.48 27.82 28.9 23.799999999999997 30-34 20.27 28.57 27.529999999999998 23.630000000000003 35-39 20.415 27.62 27.375 24.59 40-44 20.32 27.950000000000003 27.04 24.69 45-49 20.25 27.6 28.060000000000002 24.09 50-54 20.525 27.68 27.845 23.95 55-59 20.24 28.075 28.16 23.525 60-64 20.405 27.105 28.155 24.335 65-69 19.955000000000002 27.93 28.09 24.025 70-74 20.77 27.834999999999997 27.425 23.97 75-79 20.810000000000002 26.52 28.26 24.41 80-84 20.31 27.36 27.735 24.595 85-89 20.919999999999998 27.27 28.439999999999998 23.369999999999997 90-94 21.685 27.145000000000003 27.41 23.76 95-99 21.07 27.565 27.66 23.705000000000002 100-104 20.65 27.51 27.52 24.32 105-109 21.81 27.245 27.33 23.615 110-114 20.669999999999998 27.99 27.284999999999997 24.055 115-119 21.775 26.76 27.33 24.135 120-124 21.349999999999998 26.939999999999998 27.92 23.79 125-129 21.805 27.500000000000004 26.57 24.125 130-134 21.135 26.645000000000003 27.994999999999997 24.224999999999998 135-139 21.959999999999997 26.889999999999997 27.365000000000002 23.785 140-144 22.28 27.139999999999997 27.025 23.555 145-149 22.11 27.96 25.900000000000002 24.03 150-151 22.0125 27.975 25.6 24.4125 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 0.0 1 0.5 2 0.5 3 0.0 4 0.0 5 0.0 6 0.5 7 0.5 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.5 17 0.5 18 1.5 19 1.5 20 0.0 21 0.5 22 1.0 23 1.0 24 2.0 25 3.0 26 3.0 27 3.5 28 6.5 29 8.5 30 7.5 31 13.0 32 18.5 33 22.5 34 44.0 35 54.5 36 71.0 37 102.0 38 122.5 39 166.5 40 182.5 41 173.5 42 209.0 43 252.5 44 294.5 45 317.0 46 282.5 47 254.0 48 245.0 49 204.5 50 190.5 51 175.5 52 135.5 53 109.0 54 83.5 55 61.0 56 38.0 57 28.0 58 27.0 59 22.0 60 13.5 61 10.0 62 7.0 63 4.0 64 1.5 65 2.0 66 3.0 67 4.0 68 6.0 69 3.5 70 0.5 71 0.0 72 0.5 73 1.5 74 1.0 75 0.0 76 0.0 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.025 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150-151 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 58.5 #Duplication Level Percentage of deduplicated Percentage of total 1 61.32478632478633 35.875 2 21.367521367521366 25.0 3 9.82905982905983 17.25 4 3.717948717948718 8.7 5 2.0085470085470085 5.875 6 0.8119658119658121 2.85 7 0.5982905982905984 2.45 8 0.17094017094017094 0.8 9 0.0 0.0 >10 0.17094017094017094 1.2 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CTCTGCCGCCATATCGAAAACCCTGTTAAAAGAAGAAGAAAAAAGAGGGC 14 0.35000000000000003 No Hit GACAGCATGAAATGCCCTTCTACAACTTTGACACCTTAGAATACACTCCT 12 0.3 No Hit CTTCATCAATCCCAGATTTCTCTGCATCAAATGCTTTCCACCATGGTGGA 12 0.3 No Hit AGACGCAAGAGAGGAGCGCGTGGGACAGGTTCGAGAGTTGTGGCCGTTGT 10 0.25 No Hit GCCAAGGCCTCATACTTTCCAACAGTATCGGCGATTCGATCTATGCAAGG 8 0.2 No Hit GTTGAATATATTGTATCAAGTCATAGCCAGCAGTGGTCCACTTATTGTAG 8 0.2 No Hit GGCTTATATCAGATCTCATCTAGCTTGTCTTTCCACACGGCATATCAGGT 8 0.2 No Hit GAATTGAATTAATGAACTTGGTGGCAGCAAGGAAGGCGTTGTAGGAGGCT 8 0.2 No Hit CTTCAGTCTTGCACACACTAGCACAGTTGTGGCCTCTTACACATGGTCCT 7 0.17500000000000002 No Hit CTCCAACAACGTCCTTCACCAAGCTCACAAGCACACTGTGTGGCACCTTC 7 0.17500000000000002 No Hit ACCCTTCATATAGCTAGTTCTTGAAGCACTATAAATCCCTTTTTAAGTCG 7 0.17500000000000002 No Hit CTGGGCAAAAATGCGCATGCATTATCTTCTTTGACTGCAGAGATTCCTAA 7 0.17500000000000002 No Hit CCGCATAAAAACCCTTAAAACCTTCCTTAGAGACAATAAGACGGACAGCA 7 0.17500000000000002 No Hit CCCCACCAAGCAATCCTTCATAATCTGTGTCACCACATTCTCCAACAAAA 7 0.17500000000000002 No Hit CCCAAATATTCCTTCCATCAACCACTCCAGCAAAGAGGTATTTTCCAGAA 7 0.17500000000000002 No Hit GTCTTGCTATTGCAGCGTGGGTCTTGTCTAGCTTTGCTCCATCCCCCTTG 7 0.17500000000000002 No Hit CTCCAGGTCAGTTTGATCCATTGCGGGGTCAGTGCTCCAGTTCTCGTCTG 7 0.17500000000000002 No Hit GGGATCATTTTCTTCTTTAACGGTGACGACTTCTACCGGCGTGGAAGCGA 7 0.17500000000000002 No Hit CTTGCTGAGGTTGTATGGCTCTGCATTCGTCGCTCTTTCCTTGCGTTTCT 7 0.17500000000000002 No Hit GGCCTTTGCAATTCTCTTCTTTTTGTCTGAAATCCTGGCTCGCTTCCTTT 7 0.17500000000000002 No Hit CATAAACTGAAACTGTGATGAGTGAATCATTGTTTTTATTATAAAACAAG 7 0.17500000000000002 No Hit CCGGCTCTTTTTCCTTGGCTTCTTTTTTGGGTTCCTCCTTGGCCTTTGAC 7 0.17500000000000002 No Hit GTCACAAATCAACAAGCAATATTTGAACAAGTACAGAGCCAGCAAAAAAA 6 0.15 No Hit CTTCCTTTTCTCTCTCAGGATCGATGTCGGTGATGCAGTTCTTAACTTTA 6 0.15 No Hit GTTTAGTTAAATCACTGCATTCGGCTAATATCAAAAGATGGAGATTACGA 6 0.15 No Hit CTTTAGCTCCATCTGGTGTACTGGATGCTTTGAGGGAAGATGATCCAGCA 6 0.15 No Hit CATCAACTTGCTCATATTAGTTGCTTCCAATGCCTTAGTAGCATCTTCCT 6 0.15 No Hit GCCTCCTCCTCCTCCTTTGGTTGGTTTCTTGGTCACAGAGAGCTTGGCGC 6 0.15 No Hit CACGACTTTCTTCTGTAACCTCTACAGATGGGTCCCCCATCTTCTGTGGA 6 0.15 No Hit GGCCCAGCAAGGACACCAGAGAGGGTTTCAACACCAAAAAAGATCCCAAC 6 0.15 No Hit GCACGCTTGTGGACACGAAGCTCAAATCTGTCCCATGTATTGGTTCCTTC 6 0.15 No Hit GCCTGCTGATAAGCTGGTGCGAAACCATTGCCATACCCTCCATATGCTGG 6 0.15 No Hit GGTCCTTTATCATTTTGCCTAATCTGTGCATTATGCTTGGCAGACCCATA 6 0.15 No Hit GGATTTGGATCAGTCAGCAATGAGCAAATGGAGAGCAGTACCTTAGAAAT 6 0.15 No Hit TTCCCGTCCTCGCCGGTGTGACCTGCAAAATACCCAAAAAAACGCAACAG 6 0.15 No Hit ATCCAATCCTGCCAAAACCAATGGATTCAGCAACTCGCCGACTGGAATCC 6 0.15 No Hit GTTTTTCATTGGAAAGCCCATTCTCTATCTCCTCCGCAACGGCATTACAA 6 0.15 No Hit GTTTGGGCTAGGCTGCTGGCCAGTAGAATAACCTTGCTGCCCCATTGCAG 6 0.15 No Hit CTTTGGTATTAGCTTTCAGAAGCCTTTCATGGATAGTTGTCGCTTCGTTG 6 0.15 No Hit GGTGTGGCCCCCAGAGAGAGCTACAATATCCTTATCACTGAGGCCCATGT 6 0.15 No Hit GCTTGGTGTTGGTGTTGGTGTTGGTGTTGGCTTTGGCTTTGGCTTTGGCT 6 0.15 No Hit GTTGTTTAGTCGAAGGAATACGAGAGACTTCAACTTGGCAAAAGATTTAG 5 0.125 No Hit CCGTCTGTGTGGCATCTAGAAGAAGAAATATCCTTTCTCTTACAGGCAAC 5 0.125 No Hit GTACGAGTAGCTGCAAGATTTTAATCAGAAACCCAGGCTTATCGTCTTTA 5 0.125 No Hit CCTCTCCAAACTGAAGTCTATTTCCATCTTCATGAACACATTGAAACTTG 5 0.125 No Hit CTCCACACATCCTCGTAATCTTCTTTGCAAAGAAGCTTTCTTTTTGGTAT 5 0.125 No Hit GCCCCCTCAGGGATGCCCCTATAAACGGAAGGAACATGCCTGCCACAACC 5 0.125 No Hit GGCGAGATCCGAGTTGGAGCAACAATACAGGGCCATGTTTACTAGAGATT 5 0.125 No Hit TGGCCATTTCCTGCGCTACAATTGTCAGGTTCTGGTGAAGAGGGGGAGGA 5 0.125 No Hit GCTGCGATAAGAGAGATTATGGCCCATGGAGTATGGAAATACTCTAGTTT 5 0.125 No Hit GCCATTCAGAGCAGGTAGCACCTTCCCAACAGCCTTTGCAGCTCCAGTGC 5 0.125 No Hit GTACAGTGCGACCAGAAGATATGGAGCCAGAGTAAGCTATGGCAGCTATG 5 0.125 No Hit GTCCAGTTTAATGCACCATAACCAGTTCCATAAGCCAAAGCTCCAATAAC 5 0.125 No Hit GTCCCGACTTCAGTGGCTTTCTTGCGTATAGAAGCAGCAGACACATCACA 5 0.125 No Hit GGTGCTTGAATTGTTGGCATAGACGAAGTTGTTATTGCATTGACACTTGG 5 0.125 No Hit TCTGTAGCGACACGGAGACCATTAGGGAGGGTAGTGATGCGGGTCTCGGG 5 0.125 No Hit CATCATTGTAAGACTTATCCTTTGATGAGAAATGCCTCCGCTCACGCTCT 5 0.125 No Hit GATAACAAAGCTTTGTGAGAAGGAGGAAGCTTCCTAAACGATCTTTCGTA 5 0.125 No Hit ACCAGCCCCATCACCGCTTTTGCTGCTATTTTCCTTGGACATCGAACTAT 5 0.125 No Hit CCATGCATGCTTATTTACAATACTTGTTGAACCATACTGCAATGTTGAGG 5 0.125 No Hit GGCGAAACGAGTTTAGGGAGCCATTTGAGAGGGATTCCCAGTCAACTTTC 5 0.125 No Hit GCAGGATAATCACTTAGACGCCTTCATGGATGATACCAGCTCGCTTAACT 5 0.125 No Hit GATTGTTCATCTCCATCTATCTGGGAGATGATGTTACACACAATGTTCTC 5 0.125 No Hit CTCGGTAAATGGAAAAGCATCTGCCCCGTAAGCTGTCAAGTGCATCCGAG 5 0.125 No Hit CATAGATTGATTCTCCTCCGGTTCCATTCCCCGCAGTAAAATCCCCTCCT 5 0.125 No Hit CCATGATTTACAAATTCGCTATTGCTGCTCATGCCACCAGTGCTGCTAGC 5 0.125 No Hit AGATTACCAGCAGACTTTCCTTTCTTGATTAAACTAGATTGGTGTGGAGT 5 0.125 No Hit ATAGCTTGGTTGAGTTGGTGCTGGTTGAGCATAACCCTGCTGGCTGGCTG 5 0.125 No Hit GGCCAATTTGGGTCCTTTCCACCTAATCTCCACCGTCAACTTCTCCGACT 5 0.125 No Hit CCATCTTCTAACTATGCTGCTTGCATGTGGGTGTTTGACTCTGAATTCAA 5 0.125 No Hit GTAGAATTTGTTAAAGCCATTCCGATTGCAGTCCTCTTGCACAACAATAA 5 0.125 No Hit CCCTACTCTATCTTTTATGATCAAGAAATTAAATCACGATTCTGGAGGCA 5 0.125 No Hit GTCCTCATTCCCTTCATTGTAATCTTCTTGCATCCCTCGACTTCAAACCT 5 0.125 No Hit GTCCTGTTTAATTATTCAGCACGGCCTATTTTGCAAAGGAACATAACTCA 5 0.125 No Hit CTCATATCATATGCGACAGTCTTTAACAAAGTAAGATCCAAGGTGGCAGC 5 0.125 No Hit CTCGGCATCTTCATCAGCTCCTTCTTCAATCTCAGGTTCTGCCGGCTCAT 5 0.125 No Hit GGAGAATCTTTCTGTTGCCATTCATCGAGCAATCTTTTGCATGGACATTA 5 0.125 No Hit CGGTCAGACACCACAACCTTCTCAACCTTGTCTCCCAGCACATCCTTGAT 5 0.125 No Hit GTCCAGTGATTAATTTCCTCACATCTTGAAGAGTGGTGTTTACATCATCC 5 0.125 No Hit GTCGGCGGAACCCTTTGCATTCACCACCGGGAAAACCTTCAGTCTTGCAC 5 0.125 No Hit GCCTCCATAACCTCAGCCCATGGCATACTTCTGTGTCCAGCTGCGAGCAG 5 0.125 No Hit TGAAAGTGGCATGTAACTCATCTGGGGAAGAAAGTCCAGCGAGAAGCGAT 5 0.125 No Hit TTTTTTTTTTTTTCATAAAGGAACAACAAATACCATATCAACACATTGAA 5 0.125 No Hit CTCATCAACAGTGATGATGTATTTTCCAGTTGAGTCTGTTGTGCCTTCCT 5 0.125 No Hit CCCAAACTAGACATAACAGCTGCAATCAAATAAGTATCTCTTTCTCTTTG 5 0.125 No Hit ACGGGCTCAGCGATCAAGTGGTCGAGAAGGGTGAGGTTGAAGTCACCGGG 5 0.125 No Hit AAACTATCAAACGGCGATAACTCTTCGTATGGGAGAACAGACGTCGGTGA 5 0.125 No Hit GTCTTGACCGAAGGTATATCCAGCACCCCTAGGTGAGATGCCCCAACCAC 5 0.125 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0 0.0 0.0 0.0 0.0 64-65 0.0 0.0 0.0 0.0 0.0 66-67 0.0 0.0 0.0 0.0 0.0 68-69 0.0 0.0 0.0 0.0 0.0 70-71 0.0125 0.0 0.0 0.0 0.0 72-73 0.037500000000000006 0.0 0.0 0.0 0.0 74-75 0.05 0.0 0.0 0.0 0.0 76-77 0.05 0.0 0.0 0.0 0.0 78-79 0.05 0.0 0.0 0.0 0.0 80-81 0.05 0.0 0.0 0.0 0.0 82-83 0.075 0.0 0.0 0.0 0.0 84-85 0.1125 0.0 0.0 0.0 0.0 86-87 0.21250000000000002 0.0 0.0 0.0 0.0 88-89 0.3125 0.0 0.0 0.0 0.0 90-91 0.4 0.0 0.0 0.0 0.0 92-93 0.4125 0.0 0.0 0.0 0.0 94-95 0.4875 0.0 0.0 0.0 0.0 96-97 0.5625 0.0 0.0 0.0 0.0 98-99 0.825 0.0 0.0 0.0 0.0 100-101 0.8875 0.0 0.0 0.0 0.0 102-103 0.9875 0.0 0.0 0.0 0.0 104-105 1.475 0.0 0.0 0.0 0.0 106-107 2.1125 0.0 0.0 0.0 0.0 108-109 2.3375 0.0 0.0 0.0 0.0 110-111 2.6624999999999996 0.0 0.0 0.0 0.0 112-113 3.0374999999999996 0.0 0.0 0.0 0.0 114-115 3.425 0.0 0.0 0.0 0.0 116-117 3.825 0.0 0.0 0.0 0.0 118-119 4.225 0.0 0.0 0.0 0.0 120-121 4.625 0.0 0.0 0.0 0.0 122-123 5.1375 0.0 0.0 0.0 0.0 124-125 5.85 0.0 0.0 0.0 0.0 126-127 6.675000000000001 0.0 0.0 0.0 0.0 128-129 7.112500000000001 0.0 0.0 0.0 0.0 130-131 7.575 0.0 0.0 0.0 0.0 132-133 8.1 0.0 0.0 0.0 0.0 134-135 8.6875 0.0 0.0 0.0 0.0 136-137 9.7875 0.0 0.0 0.0 0.0 138-139 10.625 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTAATCG 10 0.006830828 145.0 7 TAATCGT 10 0.006830828 145.0 8 CATGGCT 10 0.006830828 145.0 2 TGGCTAA 10 0.006830828 145.0 4 >>END_MODULE SRR26075371 read2 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR26075371_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.1665 37.0 37.0 37.0 37.0 37.0 2 36.3125 37.0 37.0 37.0 37.0 37.0 3 36.3695 37.0 37.0 37.0 37.0 37.0 4 36.2415 37.0 37.0 37.0 37.0 37.0 5 36.3135 37.0 37.0 37.0 37.0 37.0 6 36.3195 37.0 37.0 37.0 37.0 37.0 7 36.142 37.0 37.0 37.0 37.0 37.0 8 36.258 37.0 37.0 37.0 37.0 37.0 9 36.3805 37.0 37.0 37.0 37.0 37.0 10-14 36.3293 37.0 37.0 37.0 37.0 37.0 15-19 36.304500000000004 37.0 37.0 37.0 37.0 37.0 20-24 36.294500000000006 37.0 37.0 37.0 37.0 37.0 25-29 36.1826 37.0 37.0 37.0 37.0 37.0 30-34 36.2077 37.0 37.0 37.0 37.0 37.0 35-39 36.0912 37.0 37.0 37.0 37.0 37.0 40-44 36.0577 37.0 37.0 37.0 37.0 37.0 45-49 36.017100000000006 37.0 37.0 37.0 37.0 37.0 50-54 35.9062 37.0 37.0 37.0 37.0 37.0 55-59 35.9179 37.0 37.0 37.0 37.0 37.0 60-64 35.9437 37.0 37.0 37.0 37.0 37.0 65-69 35.83669999999999 37.0 37.0 37.0 37.0 37.0 70-74 35.7419 37.0 37.0 37.0 37.0 37.0 75-79 35.708000000000006 37.0 37.0 37.0 37.0 37.0 80-84 35.7192 37.0 37.0 37.0 37.0 37.0 85-89 35.7005 37.0 37.0 37.0 37.0 37.0 90-94 35.598 37.0 37.0 37.0 37.0 37.0 95-99 35.6328 37.0 37.0 37.0 37.0 37.0 100-104 35.5222 37.0 37.0 37.0 37.0 37.0 105-109 35.5484 37.0 37.0 37.0 37.0 37.0 110-114 35.516099999999994 37.0 37.0 37.0 37.0 37.0 115-119 35.527100000000004 37.0 37.0 37.0 37.0 37.0 120-124 35.4067 37.0 37.0 37.0 34.6 37.0 125-129 35.3414 37.0 37.0 37.0 37.0 37.0 130-134 35.3629 37.0 37.0 37.0 34.6 37.0 135-139 35.147999999999996 37.0 37.0 37.0 32.2 37.0 140-144 35.1806 37.0 37.0 37.0 27.4 37.0 145-149 35.1374 37.0 37.0 37.0 27.4 37.0 150-151 34.7315 37.0 37.0 37.0 25.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.0 1101 2 0.0 1101 3 0.0 1101 4 0.0 1101 5 0.0 1101 6 0.0 1101 7 0.0 1101 8 0.0 1101 9 0.0 1101 10-14 0.0 1101 15-19 0.0 1101 20-24 0.0 1101 25-29 0.0 1101 30-34 0.0 1101 35-39 0.0 1101 40-44 0.0 1101 45-49 0.0 1101 50-54 0.0 1101 55-59 0.0 1101 60-64 0.0 1101 65-69 0.0 1101 70-74 0.0 1101 75-79 0.0 1101 80-84 0.0 1101 85-89 0.0 1101 90-94 0.0 1101 95-99 0.0 1101 100-104 0.0 1101 105-109 0.0 1101 110-114 0.0 1101 115-119 0.0 1101 120-124 0.0 1101 125-129 0.0 1101 130-134 0.0 1101 135-139 0.0 1101 140-144 0.0 1101 145-149 0.0 1101 150-151 0.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 14 3.0 15 2.0 16 4.0 17 0.0 18 2.0 19 5.0 20 4.0 21 4.0 22 8.0 23 5.0 24 3.0 25 11.0 26 15.0 27 11.0 28 20.0 29 23.0 30 23.0 31 32.0 32 56.0 33 91.0 34 206.0 35 679.0 36 2604.0 37 189.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 43.675000000000004 21.325 10.975 24.025 2 29.9 23.75 28.349999999999998 18.0 3 24.3 27.200000000000003 28.050000000000004 20.45 4 24.55 36.175000000000004 21.2 18.075 5 26.0 36.125 21.475 16.400000000000002 6 23.05 39.1 21.3 16.55 7 23.3 20.95 35.775 19.975 8 21.95 25.55 27.35 25.15 9 22.275 26.650000000000002 29.299999999999997 21.775 10-14 24.575 28.945 25.35 21.13 15-19 23.915 27.994999999999997 27.21 20.880000000000003 20-24 24.95 28.084999999999997 26.83 20.135 25-29 24.495 28.815 25.369999999999997 21.32 30-34 23.200000000000003 27.255000000000003 27.58 21.965 35-39 24.12 27.205000000000002 27.755000000000003 20.919999999999998 40-44 24.3 28.02 26.845000000000002 20.835 45-49 24.675 27.51 26.3 21.515 50-54 23.77 28.975 26.985 20.27 55-59 24.535 27.515 26.840000000000003 21.11 60-64 24.43 27.92 26.715 20.935000000000002 65-69 23.549999999999997 28.32 27.18 20.95 70-74 24.104999999999997 28.595 26.505000000000003 20.794999999999998 75-79 24.025 28.555000000000003 26.755000000000003 20.665 80-84 25.014999999999997 27.425 27.229999999999997 20.330000000000002 85-89 24.060000000000002 28.375 26.32 21.245 90-94 24.22 27.705000000000002 27.0 21.075 95-99 24.38 27.79 27.185 20.645 100-104 25.324999999999996 28.63 26.119999999999997 19.925 105-109 23.799999999999997 27.825 27.505000000000003 20.87 110-114 23.985 28.384999999999998 26.69 20.94 115-119 24.37 28.165000000000003 26.31 21.154999999999998 120-124 25.14 27.650000000000002 26.229999999999997 20.979999999999997 125-129 25.8 28.925 24.94 20.335 130-134 25.045 29.299999999999997 25.75 19.905 135-139 25.729999999999997 28.405 25.974999999999998 19.89 140-144 26.700000000000003 28.720000000000002 25.19 19.39 145-149 27.205000000000002 28.515 24.834999999999997 19.445 150-151 27.675 28.512500000000003 25.112499999999997 18.7 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.5 6 1.0 7 1.5 8 1.0 9 0.0 10 0.0 11 0.5 12 0.5 13 0.0 14 0.5 15 1.5 16 2.0 17 2.0 18 1.0 19 0.5 20 0.5 21 0.5 22 1.0 23 0.5 24 0.5 25 1.5 26 2.5 27 2.5 28 2.5 29 3.0 30 4.5 31 3.0 32 6.0 33 18.5 34 27.0 35 38.0 36 64.0 37 81.5 38 106.0 39 135.0 40 166.5 41 230.5 42 259.5 43 279.5 44 272.0 45 296.5 46 294.0 47 245.5 48 251.5 49 234.0 50 219.0 51 164.5 52 115.0 53 98.5 54 70.5 55 57.0 56 53.5 57 42.5 58 31.0 59 22.5 60 16.5 61 12.0 62 7.0 63 3.5 64 1.5 65 4.0 66 5.5 67 3.5 68 6.0 69 5.5 70 1.0 71 1.5 72 1.0 73 0.0 74 0.0 75 0.0 76 1.0 77 1.0 78 0.0 79 0.0 80 0.5 81 1.0 82 0.5 83 0.5 84 1.5 85 1.5 86 1.0 87 0.5 88 0.0 89 0.5 90 0.5 91 0.0 92 0.5 93 0.5 94 0.0 95 0.0 96 0.5 97 0.5 98 0.0 99 0.5 100 2.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150-151 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 59.150000000000006 #Duplication Level Percentage of deduplicated Percentage of total 1 62.5105663567202 36.975 2 20.87912087912088 24.7 3 9.29839391377853 16.5 4 3.423499577345731 8.1 5 1.944209636517329 5.75 6 1.098901098901099 3.9 7 0.46491969568892644 1.925 8 0.2113271344040575 1.0 9 0.042265426880811495 0.22499999999999998 >10 0.12679628064243448 0.9249999999999999 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTGATAAACCTCATTCTCTCATCTCAGTCGTCTCCTTTCACCTCGCTTCT 15 0.375 No Hit GGCAAGCGATGAAGCACACAACCACCTTCACCGCTGCTGCTGCAACTGGG 12 0.3 No Hit GAGAGATTGAACTATCCATTCAATTTTATCCAACTCAGTTTTATTAGGTG 10 0.25 No Hit AGCGTCAGGAACGCAATTAACGCTGCAAACGATGCCCTGAAAGATCTGGA 9 0.22499999999999998 No Hit GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGT 8 0.2 No Hit CCTCTCTTCGCCTCCGTTCCGGCATGGGCCTCCCCGTCGGCAAACACATC 8 0.2 No Hit ACAGCAAGTGCTTCTGTAGCTTTGACATCTTTGTCAAATGAAAAGCTCTT 8 0.2 No Hit CAACACTGTGATGTTGATGCTTGACAGTGGATTACCTTGCTTCAGTAGGG 8 0.2 No Hit GTCTACTCCACCATGCTCTAGTCTCTCAAGCCCATCACATACAGGTGCCC 8 0.2 No Hit GTTTTAGAAACATCCGGTTACAGGAGGAATATGATACTCATGATCCAAAA 7 0.17500000000000002 No Hit GGTTCACTGAATCAAACTGGTGGTCCAACGAGTCTTTGTTCTACAATGGA 7 0.17500000000000002 No Hit CTACACTTCGAGGGCTTGAACTTGGTCTTGCCCATGGAGAACAGCTTGAT 7 0.17500000000000002 No Hit CTGACACTGAGAAACCCAGGATGAGGGGTCCAAAGAGGGCATCCAAAATC 7 0.17500000000000002 No Hit GTGCTAGATACTAGTAGTAGTTGCAGACAGAGAGCTAGTGTTAGACAGGT 7 0.17500000000000002 No Hit CCCAGACAGCATAGACTATGGTGCCAAGGTGTCTGTCAAAAACCCATATG 7 0.17500000000000002 No Hit ATGAGCCTACCAAGCAGAAATTGTTGAAGACTTTTCCTGAAAATCTTAGT 7 0.17500000000000002 No Hit GAGAATTCAGGCTCTCCGTTGCCATGCTATTTATTGCCAAAATGGGACCA 7 0.17500000000000002 No Hit GGGAAACAACTGGCTCATGGTTTTTTTCCACAAGACGGCCGTCTCCATTA 7 0.17500000000000002 No Hit AAAACACTAATTTCTTTGAAGTGTGTTACTGGGTTTGGGTTTGACCTGGT 7 0.17500000000000002 No Hit GAGAGCACTGGGTGCCCTGAACCTGCATCTTGCCTCAAACACTTACTTGG 7 0.17500000000000002 No Hit GGAGTAAGAATGCCAATACTCAATTATATGCAGTGATGTTGTTGGAGATG 6 0.15 No Hit GTCGAGCGTCTTTTTAGAAGATATGGGAGGATTGATAGGGTGGATATGAA 6 0.15 No Hit GGGAAGGATTCCATACCCTGAGGGCCGTGTTTACAATAATGCCGGAGCCA 6 0.15 No Hit CAAGAAGCATGGAACTTAGCTAATGGTAGTTTGCGGACAACTTTGTGTGT 6 0.15 No Hit GTTGAAGATCAAACGAAACCAATTAGTGCCCATCTCGAGAATCAACAAGA 6 0.15 No Hit ATCTCTACCGATGCTTCCATCAAGGAGATGATCGCACCTGGTGCACTTGT 6 0.15 No Hit AAATATCCTCCCCCCTTCTCTCCTCCTCTCTCTCTCTCTTTCAAACCCTA 6 0.15 No Hit TGGGAAATGGTTGAACAATCAGGATCGGAGGCTACCAGTTCAGCTAAATA 6 0.15 No Hit GCAGCGATGATGTTTAAAGTTAATGCTGGTGGGTTGTAGAATTATGATGA 6 0.15 No Hit AGCTAGTATTCACTGTAGCTTCTAAATTCAAAAGGAGCTATTAGCTTTTG 6 0.15 No Hit GCCAGACCCGACAAAAGCCTAGCAGCCGGCGGCCCTCTTGGTCATACAGC 6 0.15 No Hit GCTAAAGTTGGCAGTTGAGAAGAAGGCGGGTGCAGCAGAAGCCCAGAGAT 6 0.15 No Hit TGATTATCCTGAACCTTTCATTCGTTTGTTTCTTTGCTAGCTGGATTCAA 6 0.15 No Hit GCTTATGGAGCTATGAAGGCGCAGAAGCCGGGTTTGGAGGAGGCCCAGGA 6 0.15 No Hit CGGAATTAGAAACCCCAGATCTAAAACCCTCGACGCCCACTCCCCAAATC 6 0.15 No Hit CTCAAAACAAAAAATTAGGGTTTCTTTGCTTCCAAGATACTTTCCAATTT 6 0.15 No Hit CAAGAAGCATGGAACTTAGCTAATGATAGTTTGCGGACAACTTTGTGTGT 6 0.15 No Hit CAGCAATGTGGCCAACCCAGCTCAAGAAGGACATGCTCCTTCATATGGAG 6 0.15 No Hit GAGAGAGGTGCGATCGGTAGAGACGAAGACTAGAACGATAAAGATGGCGG 6 0.15 No Hit CAGGATCAAACACACCTTTTTCTTAGACATAACAAGTCACTTCTTCAATC 6 0.15 No Hit GCAAAGGCCACATTCAGTCAGGTGATGGAGCAACCACATGCGATGGATCC 6 0.15 No Hit CTTCAATCAGACCCTCTTCTAATAAACCCTTGGCCACTCAACAACCCAAA 6 0.15 No Hit AGACAAAGTTGCCTAACATGGGTCTCATGCTTCTCAAGAAGATTAGCCTT 6 0.15 No Hit GTTGGTGTTGAAATCACTGGTGGTCCTGAGGTTCCTTTCCACCCTGGGAG 6 0.15 No Hit GCTGATAGTCCATATGCAGGTGGGGTGTTTCTAGTAACCATCCATTTCCC 6 0.15 No Hit TGCATGTTTTTTTTTTTGGGTTGTGGTTCATTGCCTGCAATCTAATAATA 6 0.15 No Hit CCTGAAAATGCTGGAAAGCTCTGTTTAAGATTGTTTTCCCATCCAACTTA 5 0.125 No Hit ATTTTCAATGGCAAACCCTAAGGTCTACTTCGACATGACAATCGGCGGCG 5 0.125 No Hit AAATAGTGAGCTAAAAAATTCTGCAGATGGCTTTATTTTGAAGTATAGAT 5 0.125 No Hit ACTAGGTCTCAAACGAAATGGCTCGCGTGTTCATGCTTCTTGCTCTCTGT 5 0.125 No Hit GGAGGTCATACCTTGACTGTCAAGGTCCTTAATTCCATTGCTGTCCTTCC 5 0.125 No Hit GTTGGGACCCAGCTCTTGTCAGTCCCTGCACCTGGTTCCATGTCACTTGT 5 0.125 No Hit TGGTGGCCCAATGCCCCCAGCCAAGGCTGGTCGCTTGACGAATGCATATG 5 0.125 No Hit GCCAACTCAAGGAATTTGAGGGCAAGAAGCTTGTCTCTGCCACCAAGGAG 5 0.125 No Hit GAACTCGGTGGTTTCTAAAGCTCCTCACGGGTTTGAGCAGATAAAGAAAA 5 0.125 No Hit GGGACTTATGACCACTGTGCACTCTATCACTGCTACCCAGAAAACTGTTG 5 0.125 No Hit CTTCAAAGAACTAGAGAGAAGCTTTGCTTCCATCCTCACGAATTTACAAT 5 0.125 No Hit GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG 5 0.125 No Hit ATTTGCTTGTCAGAATTCGTGGAGGGTGAGGGGATTCGGGTGTTAGGGAG 5 0.125 No Hit GTCAAAGTCTCCTATGGGCCAAGATGGGCCACATATATTCTTGGCATAGT 5 0.125 No Hit GGGAGGAGTTGGTGGCATCACTAGTGGAAAGTTGCTTTCAGTTGTCTCTA 5 0.125 No Hit ATCATTAAAGAGGCGAAGAGAGAAGAAAACATAAAAAGAAACAATCAACA 5 0.125 No Hit CACGAAACTACTTCGCTTCCATTTTCGAAGGCACAAAACCGAGAAAGAAA 5 0.125 No Hit GGGCAAGACACCAAGGAGGAATTGCTGAAGAGGAATCTCCGTGAGGAGTT 5 0.125 No Hit GTTAGACAGAGAGATGGAGAAGAAATGCTATGGTCTTTTCTTGTTGCTGC 5 0.125 No Hit GCCATGGCCACCGCTGGTTTCGAAGAAGTACGAGGTGAGGCTCGTTGTTC 5 0.125 No Hit TATACATCACCAAGTGCTTCAAGTTCAACTCCAACATCAAGCATAGGGAA 5 0.125 No Hit GGAAGGAAACAAATCCTGAGTCGGAAATTCAAAATTCAAGGCATTCCTGC 5 0.125 No Hit CTCTAAGGTACTGCTCTCCATTTGCTCATTGCTGACTGATCCAAATCCTG 5 0.125 No Hit GGGCTATGTTGCAGTACGTTTGTGGAGAACCATCAAAGGAACTTCAGAAG 5 0.125 No Hit GAGAAGGTGTGATCCCTGGACTTCTTGAGCTTACTGTTCAAGGAACACCC 5 0.125 No Hit GCTCAATCCACTTCACACAATGTCGAGTATCAATTTGGCAGTACTGCTGG 5 0.125 No Hit GGATCTCTCTCGAATTAGGTTAGATAGCACTTCCATGGAGAAGCTCTTGA 5 0.125 No Hit CAGAGAGGGTCCTAGGGGTTGTGACACTGGGAGTGCCATTCATACCACCA 5 0.125 No Hit CCTCCCTTTCCTCCGAAACATAGCTTCCATCCCCACAAACCTCCCGCCTC 5 0.125 No Hit TCAACAAACCCTAAACAGCCAAACCAAAAATCATGGCTTGGAAGCGCCTT 5 0.125 No Hit GTTTTGGATGCTGTACTCAACCCACACCAATTATTGCTGTGGATGAGCCA 5 0.125 No Hit CGGGAGTCGCCGTCTATCAGGGGTACTGCTAACGTTATGGCATCACCCGC 5 0.125 No Hit GTGGAAATCCTTGATCGAAATGGAATTATAGAAAACTGGATACATGACTA 5 0.125 No Hit CTTCTCTCCTTTTTTCCCTTTCTCCTCGAAAAAAATAGTTCTTTTCATAA 5 0.125 No Hit GATGGAGTTAAACTAGAATCAAAGGTCTTGTCATTGTCTTACAGTTATGA 5 0.125 No Hit CCCATATCACACATCTCAAAGGTCAAAACACCGACCATTTTTGTTCTAGG 5 0.125 No Hit TGGCCATTCACAAATCCATGGCAACCTTGATTCTCTACTTTCTTGTGATT 5 0.125 No Hit TCAACAGGGAAAGATGCAACAAATGATTTTGAAGATGTGGGTCACAGCGA 5 0.125 No Hit CTGCCGACCAACCAGCAACGACATGATCAACCCGACAAACGGCGCCGTAG 5 0.125 No Hit CAACAAGGTTATCCTGAGCAGCAAGCTGCAAACAATGCAGGTTATGGCTA 5 0.125 No Hit CATTACAAGCCAAGCTGGTTTTCGAGAATCGAGCCGAGGGCATAGTTTGT 5 0.125 No Hit AGATAAGCTAGGCAAGATGGTTTTACTAGACAAGATGTGGGATGATGTTG 5 0.125 No Hit GGAGGTTAGGAGAGAGAACCAGTGGGCTTTTGAGTAGAAGGAAAGGGGTT 5 0.125 No Hit AAGAAAGGGAAGAGGAGGAGGAAGAAGGAGCTGTACCTGTTGCCTTGGAG 5 0.125 No Hit CGTCTAGTGCTCCACCACTGTCATCAAGGCCTAAATGGGTGCTTCCTTAC 5 0.125 No Hit GCATTGGTCTTGTTTCTAGGAGGAACATCTAGATACCGGCACTTCAAACC 5 0.125 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0 0.0 0.0 0.0 0.0 64-65 0.0 0.0 0.0 0.0 0.0 66-67 0.0 0.0 0.0 0.0 0.0 68-69 0.0 0.0 0.0 0.0 0.0 70-71 0.0125 0.0 0.0 0.0 0.0 72-73 0.037500000000000006 0.0 0.0 0.0 0.0 74-75 0.05 0.0 0.0 0.0 0.0 76-77 0.05 0.0 0.0 0.0 0.0 78-79 0.05 0.0 0.0 0.0 0.0 80-81 0.05 0.0 0.0 0.0 0.0 82-83 0.075 0.0 0.0 0.0 0.0 84-85 0.1125 0.0 0.0 0.0 0.0 86-87 0.21250000000000002 0.0 0.0 0.0 0.0 88-89 0.3375 0.0 0.0 0.0 0.0 90-91 0.425 0.0 0.0 0.0 0.0 92-93 0.4375 0.0 0.0 0.0 0.0 94-95 0.5125 0.0 0.0 0.0 0.0 96-97 0.5875 0.0 0.0 0.0 0.0 98-99 0.85 0.0 0.0 0.0 0.0 100-101 0.9125 0.0 0.0 0.0 0.0 102-103 1.0125 0.0 0.0 0.0 0.0 104-105 1.525 0.0 0.0 0.0 0.0 106-107 2.1624999999999996 0.0 0.0 0.0 0.0 108-109 2.3875 0.0 0.0 0.0 0.0 110-111 2.7 0.0 0.0 0.0 0.0 112-113 3.0625 0.0 0.0 0.0 0.0 114-115 3.45 0.0 0.0 0.0 0.0 116-117 3.8625000000000003 0.0 0.0 0.0 0.0 118-119 4.262499999999999 0.0 0.0 0.0 0.0 120-121 4.65 0.0 0.0 0.0 0.0 122-123 5.1625 0.0 0.0 0.0 0.0 124-125 5.8875 0.0 0.0 0.0 0.0 126-127 6.6625 0.0 0.0 0.0 0.0 128-129 7.0625 0.0 0.0 0.0 0.0 130-131 7.6375 0.0 0.0 0.0 0.0 132-133 8.1875 0.0 0.0 0.0 0.0 134-135 8.8125 0.0 0.0 0.0 0.0 136-137 9.925 0.0 0.0 0.0 0.0 138-139 10.7875 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AGGGTAA 10 0.006830828 145.0 9 AAGGGTA 10 0.006830828 145.0 8 GAAGGGT 10 0.006830828 145.0 7 >>END_MODULE Read 879397 spots for SRR26075371.sra Written 879397 spots for SRR26075371.sra Read 879397 spots for SRR26075371.sra Written 879397 spots for SRR26075371.sra Read 879397 spots for SRR26075371.sra Written 879397 spots for SRR26075371.sra Read 879397 spots for SRR26075371.sra Written 879397 spots for SRR26075371.sra Read 879397 spots for SRR26075371.sra Written 879397 spots for SRR26075371.sra Read 879397 spots for SRR26075371.sra Written 879397 spots for SRR26075371.sra Read 879397 spots for SRR26075371.sra Written 879397 spots for SRR26075371.sra Read 879397 spots for SRR26075371.sra Written 879397 spots for SRR26075371.sra Read 879397 spots for SRR26075371.sra Written 879397 spots for SRR26075371.sra Read 879397 spots for SRR26075371.sra Written 879397 spots for SRR26075371.sra Read 879397 spots for SRR26075371.sra Written 879397 spots for SRR26075371.sra Read 879397 spots for SRR26075371.sra Written 879397 spots for SRR26075371.sra Read 879397 spots for SRR26075371.sra Written 879397 spots for SRR26075371.sra Read 879397 spots for SRR26075371.sra Written 879397 spots for SRR26075371.sra Read 879397 spots for SRR26075371.sra Written 879397 spots for SRR26075371.sra Read 879397 spots for SRR26075371.sra Written 879397 spots for SRR26075371.sra Read 879397 spots for SRR26075371.sra Written 879397 spots for SRR26075371.sra Read 879397 spots for SRR26075371.sra Written 879397 spots for SRR26075371.sra Read 879412 spots for SRR26075371.sra Written 879412 spots for SRR26075371.sra Read 879397 spots for SRR26075371.sra Written 879397 spots for SRR26075371.sra SRR ids: ['SRR26075371.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_0f7xnol3 SRR26075371.sra spots: 17587955 blocks: [[1, 879397], [879398, 1758794], [1758795, 2638191], [2638192, 3517588], [3517589, 4396985], [4396986, 5276382], [5276383, 6155779], [6155780, 7035176], [7035177, 7914573], [7914574, 8793970], [8793971, 9673367], [9673368, 10552764], [10552765, 11432161], [11432162, 12311558], [12311559, 13190955], [13190956, 14070352], [14070353, 14949749], [14949750, 15829146], [15829147, 16708543], [16708544, 17587955]] SRR26075371 file size 6489570 SRR26075371 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o SRR26075371 SRR26075371_1.fastq SRR26075371_2.fastq Input file: SRR26075371_1.fastq Paired file: SRR26075371_2.fastq trimmed: SRR26075371-trimmed-pair1.fastq, SRR26075371-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Wed Feb 12 03:19:34 2025 >> started Wed Feb 12 03:19:57 2025 >> done (22.616s) 17587955 read pairs processed; of these: 65 ( 0.00%) short read pairs filtered out after trimming by size control 24414 ( 0.14%) empty read pairs filtered out after trimming by size control 17563476 (99.86%) read pairs available; of these: 2760298 (15.72%) trimmed read pairs available after processing 14803178 (84.28%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 18 8 0.00% 19 4 0.00% 20 12 0.00% 21 5 0.00% 22 7 0.00% 23 9 0.00% 24 10 0.00% 25 25 0.00% 26 18 0.00% 27 9 0.00% 28 17 0.00% 29 19 0.00% 30 12 0.00% 31 15 0.00% 32 28 0.00% 33 17 0.00% 34 20 0.00% 35 21 0.00% 36 22 0.00% 37 23 0.00% 38 43 0.00% 39 28 0.00% 40 27 0.00% 41 52 0.00% 42 47 0.00% 43 49 0.00% 44 28 0.00% 45 64 0.00% 46 58 0.00% 47 41 0.00% 48 54 0.00% 49 84 0.00% 50 80 0.00% 51 118 0.00% 52 114 0.00% 53 99 0.00% 54 126 0.00% 55 141 0.00% 56 136 0.00% 57 181 0.00% 58 193 0.00% 59 269 0.00% 60 282 0.00% 61 367 0.00% 62 351 0.00% 63 394 0.00% 64 469 0.00% 65 597 0.00% 66 536 0.00% 67 669 0.00% 68 733 0.00% 69 860 0.00% 70 981 0.01% 71 1159 0.01% 72 1280 0.01% 73 1465 0.01% 74 1660 0.01% 75 1837 0.01% 76 2203 0.01% 77 2440 0.01% 78 2675 0.02% 79 2869 0.02% 80 3400 0.02% 81 3809 0.02% 82 4465 0.03% 83 5022 0.03% 84 5628 0.03% 85 5991 0.03% 86 6535 0.04% 87 7316 0.04% 88 8183 0.05% 89 8660 0.05% 90 9453 0.05% 91 10604 0.06% 92 11324 0.06% 93 12326 0.07% 94 13657 0.08% 95 14676 0.08% 96 15581 0.09% 97 16795 0.10% 98 17173 0.10% 99 18207 0.10% 100 19246 0.11% 101 20482 0.12% 102 21989 0.13% 103 22828 0.13% 104 24723 0.14% 105 26017 0.15% 106 27279 0.16% 107 28443 0.16% 108 29027 0.17% 109 30812 0.18% 110 31199 0.18% 111 33205 0.19% 112 33812 0.19% 113 34524 0.20% 114 36552 0.21% 115 38413 0.22% 116 39207 0.22% 117 41226 0.23% 118 42757 0.24% 119 42860 0.24% 120 43503 0.25% 121 45815 0.26% 122 45551 0.26% 123 48051 0.27% 124 48683 0.28% 125 50196 0.29% 126 51708 0.29% 127 53042 0.30% 128 53914 0.31% 129 54635 0.31% 130 56846 0.32% 131 56861 0.32% 132 58671 0.33% 133 59155 0.34% 134 60465 0.34% 135 61682 0.35% 136 63499 0.36% 137 63412 0.36% 138 66466 0.38% 139 67229 0.38% 140 67735 0.39% 141 68759 0.39% 142 70234 0.40% 143 70333 0.40% 144 71891 0.41% 145 73369 0.42% 146 73445 0.42% 147 74769 0.43% 148 75652 0.43% 149 76777 0.44% 150 78384 0.45% 151 14803178 84.28% 17563476 reads passed initial QC criterion=sequence-density sequence-density=0.33 sequence-density-rank=1 fanout-score=2.88 fanout-score-rank=33 prefix-density=0.44 prefix-fanout=2.1 sequence=CACTTGCAGCCATTCTC criterion=fanout-score sequence-density=0.04 sequence-density-rank=37 fanout-score=115.66 fanout-score-rank=1 prefix-density=0.36 prefix-fanout=11.4 sequence=CATCACCATCCCTGATTGATCTTTGATTCACAACAAGACCAACCAACCGTACAATGTTTACATAACTTGGGATAATCTCACAAGAAAGAAAGATCAATACAAAACTAGTCAGCTCAGGATGTTCCAGGCACGGTAGAACCGTAGAACCATAACAACAAGAGACATATTGCAGATGAGTACTGAAAAACAAAACACAGTACGTATTTACATGGGCAACCTTGGTTGAAGGCAACCTCATCAACGATGCTCGCTCTTCGTCTCTCCACTGTACATCCAGTCATAGACAGTGGGTGTGTTAGGCTGTGGCTTGTCAAAAACATGAGCACCAATATTCTTAGTAGCAAGGTTGCTACCAGGGTGGAACACGCTCCTCCAAACATTGCTACGCGCCGACACTGGGGTTGTAGGGGTCACTGGTGTCGTCGGTGTCCCTGGAGTTCCTGGCATAGTCATGGACCTCTGAAACTTATTAACAGGACTGCTCCCCTCTCCGACGTCAATATCTTTGATG criterion=sequence-density sequence-density=0.37 sequence-density-rank=1 fanout-score=2.81 fanout-score-rank=27 prefix-density=0.38 prefix-fanout=2.8 sequence=ATGTACCCTGAC criterion=fanout-score sequence-density=0.01 sequence-density-rank=34 fanout-score=118.15 fanout-score-rank=1 prefix-density=0.21 prefix-fanout=5.7 sequence=AGAGAAAAGATAAGCTAGGCAAGATGGTTTTACTAGACAAGATGTGGGATGATGTTGTTGCTGGACCTCAGCCAGAACGTGGCCTTGGCAAGCTTAGAAAGATCAGCACCAGACCACTTAACATCAAAGATATTGACGTCGGAGAGGGGAGCAGTCCTGTTAATAAGTTTCAGAGGTCCATGACTATGCCAGGAACTCCAGGGACACCGACGACACCAGTGACCCCTACAACCCCAGTGTCGGCGCGTAGCAATGTTTGGAGGAGCGTGTTCCACCCTGGTAGCAACCTTGCTACTAAGAATATTGGTGCTCATGTTTTTGACAAGCCACAGCCTAACACACCCACTGTCTATGACTGGATGTACAGTGGAGAGACGAAGAGCGAGCATCGTTGATGAGGTTGCCTTCAACCAAGGTTGCCCATGTAAATACGTACTGTGTTTTGTTTTTCAGTACTCATCTGCAATATGTCTCTTGTTGTTATGGTTCTACGGTTCTACCGTGCCTGGAA SRR26075371 testing PE reads STAR mapping to Ensembl genome Started job on | Feb 12 03:20:37 Started mapping on | Feb 12 03:20:37 Finished on | Feb 12 03:23:00 Mapping speed, Million of reads per hour | 442.16 Number of input reads | 17563476 Average input read length | 294 UNIQUE READS: Uniquely mapped reads number | 16075227 Uniquely mapped reads % | 91.53% Average mapped length | 292.88 Number of splices: Total | 15317879 Number of splices: Annotated (sjdb) | 14961489 Number of splices: GT/AG | 15035720 Number of splices: GC/AG | 219738 Number of splices: AT/AC | 15006 Number of splices: Non-canonical | 47415 Mismatch rate per base, % | 0.39% Deletion rate per base | 0.03% Deletion average length | 3.14 Insertion rate per base | 0.02% Insertion average length | 2.65 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 570093 % of reads mapped to multiple loci | 3.25% Number of reads mapped to too many loci | 72925 % of reads mapped to too many loci | 0.42% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 4.57% % of reads unmapped: other | 0.25% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 918156 918156 918156 N_multimapping 570093 570093 570093 N_noFeature 395341 15884073 489972 N_ambiguous 185649 948 88487 UnstrandedReadsAssigned:15494237 PositiveStrandReadsAssigned:190206 NegativeStrandReadsAssigned:15496768 Dataset is classified negative stranded MeadianReadLen=151 20thPercentileLength=151 echo kmer=147 SRR26075371 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,400 [index] number of k-mers: 62,057,036 [index] number of equivalence classes: 130,681 [quant] running in paired-end mode [quant] will process pair 1: SRR26075371-trimmed-pair1.fastq SRR26075371-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 17,563,476 reads, 15,743,157 reads pseudoaligned [quant] estimated average fragment length: 213.099 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,217 rounds 52401 SRR26075371.ke.tsv 34699 SRR26075371.se.tsv 87100 total ==> SRR26075371.ke.tsv <== target_id length eff_length est_counts tpm Potri.005G200100.1.v4.1 2018 1805.9 1346 40.6409 Potri.005G024800.1.v4.1 1035 822.901 908 60.1659 Potri.004G059700.1.v4.1 961 748.901 25 1.82024 Potri.007G009000.2.v4.1 1416 1203.9 0 0 Potri.003G141000.2.v4.1 2943 2730.9 749 14.9551 Potri.016G087400.1.v4.1 270 89.572 1522 926.52 Potri.015G069301.1.v4.1 564 353.717 0 0 Potri.010G195200.1.v4.1 1773 1560.9 282 9.85113 Potri.012G127500.1.v4.1 977 764.901 5121 365.058 ==> SRR26075371.se.tsv <== Potri.001G166300.v4.1 0 Potri.001G448400.v4.1 629 Potri.001G233950.v4.1 0 Potri.001G122700.v4.1 256 Potri.001G212900.v4.1 0 Potri.001G182400.v4.1 3 Potri.001G256600.v4.1 0 Potri.001G040500.v4.1 0 Potri.001G416900.v4.1 0 Potri.001G452600.v4.1 719 SRR26075371 completed mapping pipeline successfully