Starting /dee2/code/volunteer_pipeline.sh SRR26075372 current disk space = 3052388155392 free memory = 1500876544 SRR26075372 SRAfilesize de3f76c8d7ceb1ab0e87b1f357ac61a7 SRR26075372.sra SRR26075372.sra file validated SRR26075372 is paired end SRR26075372 is conventional basespace SRR26075372 read1 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR26075372_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.602 37.0 37.0 37.0 37.0 37.0 2 36.5975 37.0 37.0 37.0 37.0 37.0 3 36.6235 37.0 37.0 37.0 37.0 37.0 4 36.6755 37.0 37.0 37.0 37.0 37.0 5 36.7285 37.0 37.0 37.0 37.0 37.0 6 36.652 37.0 37.0 37.0 37.0 37.0 7 36.643 37.0 37.0 37.0 37.0 37.0 8 36.681 37.0 37.0 37.0 37.0 37.0 9 36.6715 37.0 37.0 37.0 37.0 37.0 10-14 36.636 37.0 37.0 37.0 37.0 37.0 15-19 36.646699999999996 37.0 37.0 37.0 37.0 37.0 20-24 36.5963 37.0 37.0 37.0 37.0 37.0 25-29 36.5139 37.0 37.0 37.0 37.0 37.0 30-34 36.505700000000004 37.0 37.0 37.0 37.0 37.0 35-39 36.4493 37.0 37.0 37.0 37.0 37.0 40-44 36.3705 37.0 37.0 37.0 37.0 37.0 45-49 36.2946 37.0 37.0 37.0 37.0 37.0 50-54 36.3131 37.0 37.0 37.0 37.0 37.0 55-59 36.20459999999999 37.0 37.0 37.0 37.0 37.0 60-64 36.1983 37.0 37.0 37.0 37.0 37.0 65-69 36.1591 37.0 37.0 37.0 37.0 37.0 70-74 36.188900000000004 37.0 37.0 37.0 37.0 37.0 75-79 36.103300000000004 37.0 37.0 37.0 37.0 37.0 80-84 36.144499999999994 37.0 37.0 37.0 37.0 37.0 85-89 36.032500000000006 37.0 37.0 37.0 37.0 37.0 90-94 36.0283 37.0 37.0 37.0 37.0 37.0 95-99 36.0053 37.0 37.0 37.0 37.0 37.0 100-104 35.9352 37.0 37.0 37.0 37.0 37.0 105-109 35.8668 37.0 37.0 37.0 37.0 37.0 110-114 35.7042 37.0 37.0 37.0 37.0 37.0 115-119 35.6045 37.0 37.0 37.0 37.0 37.0 120-124 35.795899999999996 37.0 37.0 37.0 37.0 37.0 125-129 35.5943 37.0 37.0 37.0 37.0 37.0 130-134 35.477999999999994 37.0 37.0 37.0 37.0 37.0 135-139 35.388999999999996 37.0 37.0 37.0 34.6 37.0 140-144 35.2904 37.0 37.0 37.0 32.2 37.0 145-149 35.2954 37.0 37.0 37.0 32.2 37.0 150-151 35.09975 37.0 37.0 37.0 31.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.0 1101 2 0.0 1101 3 0.0 1101 4 0.0 1101 5 0.0 1101 6 0.0 1101 7 0.0 1101 8 0.0 1101 9 0.0 1101 10-14 0.0 1101 15-19 0.0 1101 20-24 0.0 1101 25-29 0.0 1101 30-34 0.0 1101 35-39 0.0 1101 40-44 0.0 1101 45-49 0.0 1101 50-54 0.0 1101 55-59 0.0 1101 60-64 0.0 1101 65-69 0.0 1101 70-74 0.0 1101 75-79 0.0 1101 80-84 0.0 1101 85-89 0.0 1101 90-94 0.0 1101 95-99 0.0 1101 100-104 0.0 1101 105-109 0.0 1101 110-114 0.0 1101 115-119 0.0 1101 120-124 0.0 1101 125-129 0.0 1101 130-134 0.0 1101 135-139 0.0 1101 140-144 0.0 1101 145-149 0.0 1101 150-151 0.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 22 2.0 23 1.0 24 2.0 25 8.0 26 7.0 27 9.0 28 14.0 29 28.0 30 32.0 31 41.0 32 56.0 33 104.0 34 138.0 35 409.0 36 2928.0 37 221.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.370370370370374 12.662662662662663 6.331331331331332 35.63563563563564 2 20.599999999999998 13.425 35.175 30.8 3 17.724999999999998 18.975 29.525000000000002 33.775 4 24.45 24.75 24.099999999999998 26.700000000000003 5 22.650000000000002 30.225 25.275 21.85 6 20.474999999999998 34.275 22.3 22.95 7 16.725 28.525 39.15 15.6 8 17.25 27.925 29.975 24.85 9 16.85 23.95 33.875 25.324999999999996 10-14 19.095000000000002 29.59 27.965 23.35 15-19 19.46 28.115000000000002 28.015 24.41 20-24 20.51 28.105000000000004 27.395000000000003 23.990000000000002 25-29 20.225 28.410000000000004 27.615000000000002 23.75 30-34 21.13 26.400000000000002 28.87 23.599999999999998 35-39 20.28 27.295 28.305000000000003 24.12 40-44 20.52 27.589999999999996 28.82 23.07 45-49 19.900000000000002 27.76 27.71 24.63 50-54 20.76 27.68 27.675 23.885 55-59 21.065 28.005000000000003 27.63 23.3 60-64 20.72 27.060000000000002 28.27 23.95 65-69 20.5 27.845 28.585 23.07 70-74 21.335 27.634999999999998 26.93 24.099999999999998 75-79 20.565 28.03 27.77 23.635 80-84 21.68 28.060000000000002 25.835 24.425 85-89 20.59 27.994999999999997 27.595 23.82 90-94 20.865000000000002 27.134999999999998 28.199999999999996 23.799999999999997 95-99 20.755000000000003 28.075 27.22 23.95 100-104 21.3 27.474999999999998 27.375 23.849999999999998 105-109 20.5 27.12 28.525 23.855 110-114 21.255 26.96 28.21 23.575 115-119 21.990000000000002 28.03 26.705000000000002 23.275000000000002 120-124 21.61 28.21 26.245 23.935000000000002 125-129 21.285 27.860000000000003 27.48 23.375 130-134 21.575 27.48 27.05 23.895 135-139 21.675 27.735 26.715 23.875 140-144 21.240000000000002 26.825 27.05 24.884999999999998 145-149 21.63 27.63 26.195 24.545 150-151 21.825 28.625 24.8625 24.6875 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.5 17 1.0 18 0.5 19 0.0 20 0.5 21 1.5 22 1.5 23 2.5 24 3.5 25 8.0 26 8.0 27 4.0 28 7.5 29 8.0 30 15.5 31 19.0 32 16.5 33 31.0 34 49.5 35 55.5 36 66.5 37 87.0 38 113.0 39 146.0 40 174.0 41 224.0 42 252.0 43 241.5 44 252.0 45 251.5 46 249.0 47 263.0 48 286.0 49 246.5 50 183.0 51 156.5 52 119.0 53 95.0 54 80.5 55 67.0 56 50.0 57 41.0 58 33.5 59 23.5 60 17.5 61 13.5 62 7.0 63 3.0 64 5.5 65 3.5 66 2.0 67 2.5 68 4.0 69 4.5 70 2.0 71 0.0 72 0.0 73 0.0 74 0.0 75 0.0 76 0.0 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.1 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150-151 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 55.275 #Duplication Level Percentage of deduplicated Percentage of total 1 57.25915875169606 31.65 2 22.388059701492537 24.75 3 10.990502035278155 18.224999999999998 4 4.477611940298507 9.9 5 3.0755314337403887 8.5 6 0.9045680687471733 3.0 7 0.5427408412483039 2.1 8 0.13568521031207598 0.6 9 0.09045680687471733 0.44999999999999996 >10 0.13568521031207598 0.8250000000000001 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CAAGGCGCCTGGAAACCTGGGTGAAATCGAGCAGCGAAGCAATCAATCCA 13 0.325 No Hit GGAGCTTGAATCATGGAAACAGACTTAACAGACTCTATTTGTTGTGTACT 10 0.25 No Hit CGCCCTTTGACCAAATTGATATTCGAGTCCACCTCATCAGCACCGTCGAT 10 0.25 No Hit GTGCAAAGTGGCACCTATCCCCAAAAGTGCAAGATCCTTTGTTGAAGTTC 9 0.22499999999999998 No Hit CTGATCGGTGATACTTGTATATTGAGGTAAGTTTGTTTGATCTTTAGCTT 9 0.22499999999999998 No Hit GGAAGTTAAGAGAGGCAAAGATGAACCACCCAAAAGGGCTTACTGTAGAT 8 0.2 No Hit CCCAGAAGAAAGGCATGACCTTCCCTTTGCCTTGATGACTTGCCTTGTGT 8 0.2 No Hit CCAGCTAACAATATCATACAGGTGAGCACGTTAATCTTGTGTAGACCCTG 8 0.2 No Hit ATCGCTGTCACTTGAGATGAAGATCACCTCAAATGCATTGTCTTTTGCTT 7 0.17500000000000002 No Hit GTCGGCTGAGTTCCATTAACCTCTGAACTTACAAGATTATATACAAAAGA 7 0.17500000000000002 No Hit CCCCAATCTTCTTGTGATGTCAATGATCCTTCAGCCGGTTCCACCATACT 7 0.17500000000000002 No Hit GCCTGTTTTTTCTTCAATTTCTTTTCCTTTTCAGTTGCTCCACGTTTAGC 7 0.17500000000000002 No Hit GTTGATGAATTACAGATGCCCATATGCAAATACAATGCACACTGCAAGGA 7 0.17500000000000002 No Hit GTGCTTCTTCTGCTTTCTCTTCTGTCTTACCTCTTGCTTCTTTCATTCCA 7 0.17500000000000002 No Hit GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGAGTTATCTCGTAT 7 0.17500000000000002 TruSeq Adapter, Index 6 (97% over 36bp) CAGGGTTTTAGGAAGGGAATTCAATCTCCGTTTTGCAGCAACTTGAACCT 7 0.17500000000000002 No Hit ACCATATCCACCAGGAGCCATTCTTCCATTTGGCCTAAAATCATCTTCCT 7 0.17500000000000002 No Hit CCGGGGAACAGTCGCATGACGAGGCAGTTTCCAGAAACGTGTATCACATC 7 0.17500000000000002 No Hit GTACTGTTCTCCCGGTCCAAAGTCTCTCTAACAGCACGATAGAATTCCAC 7 0.17500000000000002 No Hit CTCATTGGAACCAATAGTATCAATTCCATGGTCTCTGAAAACTTCTTTTA 7 0.17500000000000002 No Hit ATTGCAGATTGCATAAATCACACATGGTTTCTGGCAGGGACTCCAACACC 6 0.15 No Hit GCCCATACCAGATTTGAAACCGTTTCCATATTGACCATAGAATTTGTTTG 6 0.15 No Hit CGGTCGGCCATCAGGAAACACTGGAGCGAGCACCTTCTCTTTGTTATTTG 6 0.15 No Hit ATCTGCAGCTCCCACCAACCAGATTTTCTCCCTAAAACCCTCCAATAAAC 6 0.15 No Hit GTCTAACCATGATATCGTCTTTTGCTTTGGGTCGGTGAAGAGGAACTCAA 6 0.15 No Hit TAGTTATCGACAACTTCAATGAGGGTGGGCTCATAGTCGTTAGGAGCCTC 6 0.15 No Hit GCATGCGGGGCTTCTCCCATGAAGCAGGACCAGGGCCATAGAAAGGACCC 6 0.15 No Hit CCTTCCGCCATAAAAGCTTTCGCAGAATTATGCAATTTTCTCTTCAGCAA 6 0.15 No Hit CCTCTGTCTTAAGTGCCAGCAGGGCCTTCCTCTTCTCTTCCAGCACCTTC 6 0.15 No Hit AGCGGCGGTCCTCCAGCCAAAAGACTCGTCCAAAGCCATACACTCTTCGC 6 0.15 No Hit ACAACAAGTTCAAAAACAGAAATATCAGGGGAAAAAAACAAGGGAAAGCC 6 0.15 No Hit GTGCATCCCACTTCAGCCGTGTAGTCAGCCTTCTTGCTGATTCAACAAGT 6 0.15 No Hit ACCGAATTAAATACATCACAAAAATGCAACCACATCAACCTTGTTTCCCT 6 0.15 No Hit GGTGAACTTGGTTGCATCATAATTCATCAACGTCGACCCATGATGAATTG 6 0.15 No Hit TTTCAATTTGGTAACTTCCTGCACAACTATTTCAACATTCTCGAGCTTGT 6 0.15 No Hit GGCGACAAGTTGACTTGCTCAAGTGCCCGAGATTGTAATTCAGCTGGATA 6 0.15 No Hit GCAGCATACTCGCCCCACTTTGTGTTGACAATGTGTTAACTTTGGTTGGT 6 0.15 No Hit ATCAAAATCCCTTATCTGTGCACGCAAATGCTGAATGTCACCACATGCCT 6 0.15 No Hit GTTCATATATTTCGATCCACACGGGCGGCAAAGAAAGCTTCAAGCCTGAT 6 0.15 No Hit CACCATTCACCATCTCCCCTTCCATTACAACCTCAGAAGATCCAAAATCC 6 0.15 No Hit CAGCACATCAGAGGTTTCCCTCTTGTCAAAGACAGCCCATTTGAATCTCA 5 0.125 No Hit GGCAAAACGGCACCACCGACATAAACGGCTTCAGCAGATTACGTGTCACC 5 0.125 No Hit TGCCACAATAAATGTGGAGTCTTGAGTAAGCTTCTTCATTTTCCTAAAAG 5 0.125 No Hit GCCATTCCTTCAACCCAATGCCAGCCTTTACCATGAATCCATATTCTTTT 5 0.125 No Hit CTCCCTCAAAAATTTCACCATTGGAGTCCGGAAACTCCGGCGAATCATAT 5 0.125 No Hit GTGGGATTGAACAAACTGCTATTCACCACAAGTATTCCTATGTCTCTAGG 5 0.125 No Hit GGAAGAAGACAGCGGGGGCAAAAATTTAAAGATACATTTAAAATTTCATG 5 0.125 No Hit GCCGACACCACTATCAATAATGGTCAATGTGTTGCTGGTCTTGTCAGGAA 5 0.125 No Hit CCTTCACAGTCTATGCGCACCTTGACCTCCACCGTCTGCAATTGCCTGCG 5 0.125 No Hit GTTGGTAAGCTTGAAGATATGAGAGGTTATTATGGTGCATCTGGGTTTGG 5 0.125 No Hit CCACACTTCCTCCCCGTCTGGCTGCATTTTACAAAGAAAAATCTTATGCC 5 0.125 No Hit GCAACCTTCGAGTCACAGGTCCCACTTGTCCATCGCCAACTTCACGTCCA 5 0.125 No Hit GACCAACACAAAGCTTTACAGTTCCATCTTCCAGTACTACATATAAGGCA 5 0.125 No Hit CTTCAAGCTTTTTCCCATTAAATATATCAATTCCCACAAAGTGGCACTTA 5 0.125 No Hit GCGGATTCGACTTTCGGAGGATGATTCCCATCTTGCAAAGGCTCACCGCG 5 0.125 No Hit GCTTGTTATTCATGTGATCCTGAGATATAAGCCGAACTATTTGCATTGTT 5 0.125 No Hit CAAACGGCTCTCTGGTGTCTTCAGCATTATCTGACGATGGGAGTGAAGCA 5 0.125 No Hit GGTTTTGAAGTCTGCCTTTGTAGACAAGATTAGGTGCCTTTTCACCACTT 5 0.125 No Hit CTTGTCTTTGCACGATTTTGAAGCCCCTCTTTCTTTCTCTCCATCAATTC 5 0.125 No Hit GGCTGAATGGGTGTGGGTGTCATGGCCGCCACTTGTCATGTAACCACCAG 5 0.125 No Hit GGCTTGAAAGGGTAATCAGGAGGGAAATGGATGGTTACTAGAAACACCCC 5 0.125 No Hit CCCGCTCTCTCTCCAGCTCACGTTGCTCAAACCGAGCCTCCATGTCCCTC 5 0.125 No Hit CTCTGTTACGGGCAGTGCGTGAATCACGGATGGCCCAATGAGACTCAAGA 5 0.125 No Hit GCTCTGGCTTGCTGACATGGCGGTCGAGAATAATTTGATGAATTGTCGGG 5 0.125 No Hit GTAAGGTATTTGTTAACCTTTGCAGGGGGTGTGAGGATTTTTCCAACAAT 5 0.125 No Hit TCATAATTACTTTCGTCCCCTTCAAATTCCTCTGCATCAAGCAAGTTTTC 5 0.125 No Hit CGTCAAAGTAATTGAAGACCCTTTGTAGCTGGTTGTTTCCAATTACATTA 5 0.125 No Hit GGTGTTCTCTGAGAAACCTAAGTCAGGGTACATGCCACATTTGCAGCCAC 5 0.125 No Hit GCTTCAGGTGGCCGGCAGTTGGGCGGCGGCGGCGCTGGATTCAGGTTGTT 5 0.125 No Hit CGAGCATGATAAGCCTGCCTTGCAAGAAGCAGTTCATTTGATGACCTCGT 5 0.125 No Hit GCCTACTCCAGACTATTTTATACCAACATTTGCGTGGGACTTGTGTTCTC 5 0.125 No Hit GTACAATGTTTACATAACTTGGGATAATCTCACAAGAAAGAAAGATCAAT 5 0.125 No Hit GTTGGGCTGGCGTGGCTTTTGAGCGAGCTTGAGATTTGATAGGTTTTCTT 5 0.125 No Hit CATTGATAGCGATTACCATCCATGGGCCTTACGCCCATGTTATTGATGCA 5 0.125 No Hit CTCATTTACAAGTGCAAGGATCGCAGGTACAGTTGGCTCCACACTTGCAG 5 0.125 No Hit CATCAAGCTCTCTAAGAAACCGAAACAGGACTCTATTCATCGTAAAAATC 5 0.125 No Hit GTGGATAATAATAATGTGAACTAGTATTTGAAAGTTTGGATTCTAAAAAA 5 0.125 No Hit CTCCTTCAAATCTCAGTTGCTAGATTGGCGAAGCTCAGGTCGCACAAAGT 5 0.125 No Hit AGAGGGAGTGGATTTGGGAGTACGCCTCGGAGAGAATTCAAAGGCACAAA 5 0.125 No Hit GTTACTGCTCATTAGTCCACTGGGAATATTATTCTCGGAATTTTCCATCT 5 0.125 No Hit TATTAATCTTGAGGTCCTCAATTGGGATTCTTTCCCAGTTTTGTGCGGCA 5 0.125 No Hit TTTTCCTCTTGACTGTCACCACTCACGTTCAAGATTGTTATAGAAGCAGA 5 0.125 No Hit CTCCTTTTTCCAAGCCTCTTCTTCTCTTGCTTCTTCTTCCCTGTGTGTAT 5 0.125 No Hit GGCGCACCAAAGCCTTATCTCTCATCATTTTTCTCATCGAGTATGCAAGA 5 0.125 No Hit GAGCATTTTTGTAGTTCTCGACAACATCTTCAACCACAGACAGGTTATGG 5 0.125 No Hit GTTCTGTATCACCAGTGTCATCACTCTCACTTTCATCTGGCACTTTTGGT 5 0.125 No Hit GAAGTGGAGCCTCTGTTCTTCGGAACAGCATCTGTTTTCTTTGGTTTTGA 5 0.125 No Hit GCCATTACAGGAACCAACAATTTTTGGGAACCTAATCAAACAACCCAACA 5 0.125 No Hit GCAAGCTTGTATTCGTGATTCAAAGACCCACGTCATGACACTACACTCAC 5 0.125 No Hit GTCATACAGCATACCTTGACTTAGACTATGGTAGCAGAAACAGTCCCTTG 5 0.125 No Hit CTTCAGTAAAATGGGCTCCATCCCAGTACACATAGTCACTCCTGTTGCTA 5 0.125 No Hit TGCTTGAATGCCTTCGAGATCTCCTCAATCGGAACCACTGGCAGGTAACC 5 0.125 No Hit GGGTACATTTCGCAATGATGAGATTGAGCTCTTGAGAACTGGTGAAATTG 5 0.125 No Hit ACACGACTCTATATTTGTCTTCCCCACACGTGGATGATGTTCGTGCCTGT 5 0.125 No Hit CGTAGTTGTAGTTCCATGAAAGCTGGACAGGACCTCTGCCATAATATTGC 5 0.125 No Hit CCAAGTGCAAATTCCTCAAGACCTCCTTCGTATCTTTCAATGAGCTTTTT 5 0.125 No Hit CCCCTCTTCCTTCTCCAACAGCAGTCAAATCTGTGACTGGCGTCTGCCCC 5 0.125 No Hit ACTGTTGTTAAACATGCTGTCAATCATTTGATCCAACCGTGATAGTAGTT 5 0.125 No Hit GTTTGCTACTATTTCGGTAACCATACTGCAGCCCGCTACTTTCATTCTTG 5 0.125 No Hit GGAAGCTGGAACTGTATTGCAAATTTGAAACTGCAATTATTCAAGTCTTA 5 0.125 No Hit CTTTCAGGGGCATTAGCAACATGAGTGGGCTTTTCACCAGTGATCAACTC 5 0.125 No Hit GCACAAACCAATTTGTGCGCAAATTTTTTGCGGTTGGTCCTTTGCTAAAA 5 0.125 No Hit GCCCTCTTTGTTACAGCATCCCCGCCAGGATCAGATGATATAGAAGTTCG 5 0.125 No Hit CCTGTTGTTGCTGTTTAAGCCTCTGCTGTTGCATTTTTAGAAAGAAAGAG 5 0.125 No Hit CTTCAGTTGGCAGGAGCTTTCTTGCAGTGATTCTCAAGCAATTCCATTGT 5 0.125 No Hit GCCTCCGACTCCGTCGCCGTCGATCTGCTGAATTTCTTCATGGCCCCTTT 5 0.125 No Hit GGCGGAGTGTTTCACGCGTTAGCTGGGCCCCTGATCCGCGTAGACCAAGG 5 0.125 No Hit TATGACAGAAAATTGTAGGGATTGAACCAGCATGAACCATGAAAAGGTTT 5 0.125 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.025 0.0 0.0 0.0 0.0 64-65 0.05 0.0 0.0 0.0 0.0 66-67 0.075 0.0 0.0 0.0 0.0 68-69 0.1 0.0 0.0 0.0 0.0 70-71 0.125 0.0 0.0 0.0 0.0 72-73 0.125 0.0 0.0 0.0 0.0 74-75 0.175 0.0 0.0 0.0 0.0 76-77 0.2 0.0 0.0 0.0 0.0 78-79 0.2 0.0 0.0 0.0 0.0 80-81 0.2 0.0 0.0 0.0 0.0 82-83 0.21250000000000002 0.0 0.0 0.0 0.0 84-85 0.2875 0.0 0.0 0.0 0.0 86-87 0.325 0.0 0.0 0.0 0.0 88-89 0.36250000000000004 0.0 0.0 0.0 0.0 90-91 0.4 0.0 0.0 0.0 0.0 92-93 0.4 0.0 0.0 0.0 0.0 94-95 0.5375000000000001 0.0 0.0 0.0 0.0 96-97 0.575 0.0 0.0 0.0 0.0 98-99 0.6875 0.0 0.0 0.0 0.0 100-101 0.8374999999999999 0.0 0.0 0.0 0.0 102-103 1.1124999999999998 0.0 0.0 0.0 0.0 104-105 1.3 0.0 0.0 0.0 0.0 106-107 1.7875 0.0 0.0 0.0 0.0 108-109 2.125 0.0 0.0 0.0 0.0 110-111 2.3875 0.0 0.0 0.0 0.0 112-113 2.7625 0.0 0.0 0.0 0.0 114-115 3.175 0.0 0.0 0.0 0.0 116-117 3.7625 0.0 0.0 0.0 0.0 118-119 4.2625 0.0 0.0 0.0 0.0 120-121 4.85 0.0 0.0 0.0 0.0 122-123 5.387499999999999 0.0 0.0 0.0 0.0 124-125 6.0125 0.0 0.0 0.0 0.0 126-127 6.9375 0.0 0.0 0.0 0.0 128-129 7.45 0.0 0.0 0.0 0.0 130-131 7.875 0.0 0.0 0.0 0.0 132-133 8.3 0.0 0.0 0.0 0.0 134-135 8.8 0.0 0.0 0.0 0.0 136-137 9.2125 0.0 0.0 0.0 0.0 138-139 9.7 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AACAAGC 10 0.006830828 145.0 2 GTATCCT 10 0.006830828 145.0 145 ACAAGCC 10 0.006830828 145.0 3 CAAGCCA 10 0.006830828 145.0 4 CGTCTGA 45 0.008957279 48.333332 145 ACTCCAG 40 0.0076550315 18.125 140-144 >>END_MODULE SRR26075372 read2 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR26075372_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.2365 37.0 37.0 37.0 37.0 37.0 2 36.125 37.0 37.0 37.0 37.0 37.0 3 36.304 37.0 37.0 37.0 37.0 37.0 4 36.313 37.0 37.0 37.0 37.0 37.0 5 36.3425 37.0 37.0 37.0 37.0 37.0 6 36.259 37.0 37.0 37.0 37.0 37.0 7 36.328 37.0 37.0 37.0 37.0 37.0 8 36.3055 37.0 37.0 37.0 37.0 37.0 9 36.3425 37.0 37.0 37.0 37.0 37.0 10-14 36.2584 37.0 37.0 37.0 37.0 37.0 15-19 36.2162 37.0 37.0 37.0 37.0 37.0 20-24 36.1506 37.0 37.0 37.0 37.0 37.0 25-29 36.1549 37.0 37.0 37.0 37.0 37.0 30-34 36.0771 37.0 37.0 37.0 37.0 37.0 35-39 35.9362 37.0 37.0 37.0 37.0 37.0 40-44 35.9656 37.0 37.0 37.0 37.0 37.0 45-49 35.9369 37.0 37.0 37.0 37.0 37.0 50-54 35.75940000000001 37.0 37.0 37.0 37.0 37.0 55-59 35.824 37.0 37.0 37.0 37.0 37.0 60-64 35.910700000000006 37.0 37.0 37.0 37.0 37.0 65-69 35.80929999999999 37.0 37.0 37.0 37.0 37.0 70-74 35.66590000000001 37.0 37.0 37.0 37.0 37.0 75-79 35.6366 37.0 37.0 37.0 37.0 37.0 80-84 35.6714 37.0 37.0 37.0 37.0 37.0 85-89 35.6006 37.0 37.0 37.0 37.0 37.0 90-94 35.46660000000001 37.0 37.0 37.0 37.0 37.0 95-99 35.587599999999995 37.0 37.0 37.0 37.0 37.0 100-104 35.4839 37.0 37.0 37.0 37.0 37.0 105-109 35.512800000000006 37.0 37.0 37.0 37.0 37.0 110-114 35.4229 37.0 37.0 37.0 37.0 37.0 115-119 35.446999999999996 37.0 37.0 37.0 37.0 37.0 120-124 35.3348 37.0 37.0 37.0 34.6 37.0 125-129 35.2396 37.0 37.0 37.0 32.2 37.0 130-134 35.165 37.0 37.0 37.0 29.8 37.0 135-139 35.1032 37.0 37.0 37.0 29.8 37.0 140-144 35.03915 37.0 37.0 37.0 27.4 37.0 145-149 35.052949999999996 37.0 37.0 37.0 25.0 37.0 150-151 34.731625 37.0 37.0 37.0 25.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.0 1101 2 0.0 1101 3 0.0 1101 4 0.0 1101 5 0.0 1101 6 0.0 1101 7 0.0 1101 8 0.0 1101 9 0.0 1101 10-14 0.0 1101 15-19 0.0 1101 20-24 0.0 1101 25-29 0.0 1101 30-34 0.0 1101 35-39 0.0 1101 40-44 0.0 1101 45-49 0.0 1101 50-54 0.0 1101 55-59 0.0 1101 60-64 0.0 1101 65-69 0.0 1101 70-74 0.0 1101 75-79 0.0 1101 80-84 0.0 1101 85-89 0.0 1101 90-94 0.0 1101 95-99 0.0 1101 100-104 0.0 1101 105-109 0.0 1101 110-114 0.0 1101 115-119 0.0 1101 120-124 0.0 1101 125-129 0.0 1101 130-134 0.0 1101 135-139 0.0 1101 140-144 0.0 1101 145-149 0.0 1101 150-151 0.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 13 7.0 14 5.0 15 4.0 16 0.0 17 3.0 18 4.0 19 2.0 20 1.0 21 2.0 22 15.0 23 11.0 24 19.0 25 14.0 26 6.0 27 13.0 28 12.0 29 18.0 30 26.0 31 41.0 32 54.0 33 99.0 34 202.0 35 613.0 36 2609.0 37 220.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.824999999999996 24.2 9.275 20.7 2 25.95 27.325 29.075 17.65 3 23.925 27.900000000000002 30.975 17.2 4 24.625 35.975 22.6 16.8 5 24.775 37.125 22.0 16.1 6 22.725 37.175000000000004 23.05 17.05 7 20.225 23.724999999999998 37.075 18.975 8 23.025000000000002 25.624999999999996 26.450000000000003 24.9 9 22.400000000000002 25.874999999999996 29.075 22.650000000000002 10-14 25.365 28.999999999999996 24.625 21.01 15-19 24.135 28.32 27.045 20.5 20-24 24.275 27.644999999999996 26.740000000000002 21.34 25-29 24.43 29.044999999999998 25.64 20.885 30-34 23.599999999999998 27.650000000000002 26.99 21.759999999999998 35-39 24.0 27.49 27.57 20.94 40-44 23.75 27.93 27.22 21.099999999999998 45-49 23.855 27.955000000000002 26.735 21.455 50-54 23.16 28.63 27.889999999999997 20.32 55-59 24.03 27.47 27.165 21.335 60-64 24.145 29.044999999999998 26.755000000000003 20.055 65-69 23.990000000000002 28.12 27.41 20.48 70-74 23.715 28.305000000000003 27.025 20.955 75-79 24.52 27.735 26.855 20.89 80-84 23.745 28.189999999999998 27.250000000000004 20.815 85-89 24.610000000000003 28.444999999999997 26.145000000000003 20.8 90-94 24.33 29.104999999999997 26.26 20.305 95-99 24.93 27.97 27.084999999999997 20.015 100-104 25.480000000000004 28.000000000000004 26.27 20.25 105-109 24.04 27.575 27.175 21.21 110-114 23.7 28.185 27.284999999999997 20.830000000000002 115-119 24.295 29.165000000000003 26.229999999999997 20.31 120-124 24.935 28.77 25.474999999999998 20.82 125-129 25.045 29.17 25.845000000000002 19.939999999999998 130-134 25.825 28.365000000000002 26.665 19.145 135-139 26.855 27.87 26.064999999999998 19.21 140-144 26.45632281614081 28.436421821091056 26.26131306565328 18.845942297114856 145-149 26.26393959093864 27.384107616142423 27.074061109166376 19.277891683752564 150-151 25.890736342042754 29.2911613951744 25.003125390673837 19.814976872109014 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 1.0 11 1.5 12 1.0 13 0.5 14 2.5 15 3.5 16 2.0 17 1.5 18 1.0 19 2.0 20 2.5 21 2.0 22 2.5 23 2.5 24 2.0 25 2.0 26 1.0 27 6.5 28 11.0 29 10.5 30 9.0 31 8.5 32 16.0 33 22.5 34 31.0 35 43.0 36 57.0 37 68.5 38 115.0 39 162.5 40 207.0 41 265.5 42 274.0 43 265.0 44 269.0 45 273.5 46 272.5 47 259.5 48 226.0 49 194.0 50 167.0 51 149.0 52 126.0 53 96.0 54 87.0 55 64.0 56 32.5 57 24.5 58 23.5 59 20.0 60 15.0 61 11.5 62 11.5 63 8.0 64 9.5 65 8.0 66 4.0 67 3.0 68 0.0 69 0.5 70 1.5 71 1.0 72 0.5 73 1.0 74 1.0 75 0.5 76 0.5 77 0.5 78 0.5 79 1.0 80 2.5 81 4.0 82 2.0 83 0.5 84 1.5 85 1.0 86 0.5 87 0.5 88 0.0 89 0.5 90 1.0 91 0.5 92 0.0 93 0.5 94 0.5 95 0.0 96 0.0 97 0.5 98 1.0 99 0.5 100 8.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.005 145-149 0.015 150-151 0.0125 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 56.25 #Duplication Level Percentage of deduplicated Percentage of total 1 60.0 33.75 2 20.84444444444444 23.45 3 10.222222222222223 17.25 4 3.7777777777777777 8.5 5 3.2 9.0 6 0.8888888888888888 3.0 7 0.7111111111111111 2.8000000000000003 8 0.08888888888888889 0.4 9 0.08888888888888889 0.44999999999999996 >10 0.17777777777777778 1.4000000000000001 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG 23 0.575 No Hit AGGGTTAGCAGCCAAAGCTAGCCTAGCCGTACCCACACCCAAAACAATCC 13 0.325 No Hit GGAATACCTACCTCCACAAAAACCCACCAACAAGCCGTGTCTCTTGGCAT 10 0.25 No Hit GCTTCTTCTCACCACAATTTGGCTATAAAGAATGCAACAGTGATCTAAAA 10 0.25 No Hit TTCATTTCATTTGAAATCTCTCTCTCTCTCCCCCTCTCTCCCTCTCTCTC 9 0.22499999999999998 No Hit ACCCTAAAAAGAGGAACATAGAGCTTGAGGGTTCATTTGATCAGATCAGT 9 0.22499999999999998 No Hit CAAAGTCCTCGGGTTTCTTTCTGTCTGAACTAATTGCCTTCCAGCACCGA 8 0.2 No Hit CCCCATCAACATGGCTCCCTCGGCCCCATTTCCCTCCAGCAATTGTTGTA 8 0.2 No Hit AGAAGAGCTAATTCGTGAAGAAGAGGCAGCATGGCTTGCAGAAAGTGAGC 7 0.17500000000000002 No Hit GACCAAAGCTACTGTGCAAGAGAAGGTAGAGAAGATGTCTGCACATGATC 7 0.17500000000000002 No Hit GCTGCTCACAGTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGG 7 0.17500000000000002 No Hit CTCCAAGGTCCCAGTGTCCGATCTAGTTGGAAAGAACATTCTTCTTTACT 7 0.17500000000000002 No Hit AGAGCAAGTTGGTGGAATCTATGTAATTACTGCTGATCATGGCAATGCTG 7 0.17500000000000002 No Hit GTGGTTCAGCTGGACCCCATTTTGGAGCAGCTGCAGCAACTGCTGCACTT 7 0.17500000000000002 No Hit TAGCCGAAAAGTTGTTGATATTTTGAAGGGTGAAAATGTGAAATTTGGAA 7 0.17500000000000002 No Hit GTTAGTTTCTGATGTATGATGCTGGGTCGATGTGAATATGTGATGTACTA 7 0.17500000000000002 No Hit TCCTAATCCAACGTTTTTGTGGGGACCTGCGGACGTCTACTCATCTTCAT 7 0.17500000000000002 No Hit AAGTACTGTACTGTTGTGAGTAGTATCTTTCTTGATGATCATGCCTTCCA 7 0.17500000000000002 No Hit TCTACATCATCTAGAAGCTTTTCTGATAATGACCTTCTTTTGCTAACTAA 7 0.17500000000000002 No Hit AAGAATTGGAGCGGTGTTCGGGTCCGGGGATTGTTGTGAATATTGGAGAT 7 0.17500000000000002 No Hit GTGGAATTGGGTTCATCACATATGCAAGTGCAGATTCTGTGGATAATTTG 7 0.17500000000000002 No Hit GGAGACGCAACCAAGGGTGAATCAGAAACGAAACCTAGATTGTCAATTGA 7 0.17500000000000002 No Hit GATCTGTTAAGGTGATAGAACCCACCGAATCAGAAGGGAAGTGGGGAACG 7 0.17500000000000002 No Hit ATTGCTTAGTGTCTAAATCCAAACCAATCAGAAGGTTTGTTGGGTATTCG 7 0.17500000000000002 No Hit CCCAGCACTAAAAAGACGACCTGCTGCTCTTCTTCTTAGTGAAGATGATA 6 0.15 No Hit GGAATGAACTATAACTTCATTCGTCCAGATTTGATTGTCGGATCATGCCT 6 0.15 No Hit GCACTGAGCTCATTGCAGCAGACACCTGTAGGATCTGTACAAAAGAATCT 6 0.15 No Hit GAAGTGATGAGGAGGCTGAAGATTGGTCTGATGTTGATAGTTACGTGAGT 6 0.15 No Hit GTTAGACACTGCTGTAAAAAGTTTGAAGGCTGCTTTACAGTCATACATAC 6 0.15 No Hit CTGATGAACTTGCTGAGATAACTGAGGAAGTTGGCAATGAAGGTGACAAG 6 0.15 No Hit GTGAGATGGTTCTTGTAGCTGTTCAAATTTTGAGCTCAAGGAAGTGTGGA 6 0.15 No Hit GCGAGCCATTCCCGACTTACCCTAGAAGTTATGATTTAGTGCATGCGGAA 6 0.15 No Hit GTTGATTTCTGGTGATCGAGACTTCTCAAATGCTCTTCATCAGTTGCGAA 6 0.15 No Hit GTTCGACAGAGTCCAGATGACAATGGATGCACCATTGCCATAAACTATTG 6 0.15 No Hit GCTTCTGCTGGTGGATCATATGGGATGAAGTTCGGTATTCCAGAAGGGGC 6 0.15 No Hit GGAAAATATATAAAAATTGACATATCTAGCAATGGCGCAAGGCATGGCTC 6 0.15 No Hit AACGGACCGAACATGAATTCATGGCCCTGGCCACTGGAGCTGGATTCCGT 6 0.15 No Hit GATGGAGTTTCACGAGACACCTATTCAAAATTGAATGTCACTGACATTGT 6 0.15 No Hit GTTGGTTTCAATGTTAAGAATGTTGCTGTCAAGGATCTCAAACGTGGTTT 6 0.15 No Hit GTACAAAAACACTGAGATTTCTTCTTCATTTTCAAAGGCTGGCAATGCTC 6 0.15 No Hit GTTTGGCGTAGGGAGGCTGGTGGGGTCCATATTTGCTTGTCCGTTTTAAG 6 0.15 No Hit CTCATCAGAAAAGCTTAGACTCATCCGATTTAGGGTTTCGCTTCATATCA 6 0.15 No Hit CTTCGCTTAGAGCTGCACTGCCTGATTTGTTCAAGCAGTTGGCATGCATC 6 0.15 No Hit GAGATTGAGGAAGTCTTCACCACAATCACATCTACCGACACAAAAGCTAA 6 0.15 No Hit GCTGTGTTCGGACTTATTCGGGTAGGATGTATGCCGTCCAACATACAAAA 5 0.125 No Hit AAACACCAAAACCTCTCTCTCTCTCTAGAAAATAAACCACCAAATAAACA 5 0.125 No Hit ACCAGCTTGAGCAAATTCAGTTTCTAAGCAAAAGCTTTCCAGGCCCCTTT 5 0.125 No Hit GGAAAATGACGGAGGAACCAGGCACGACACCCGGCTCCGACGGCACGACA 5 0.125 No Hit GTTGTTTATTTCCAGAAGATTACAACATTCCAATCGAGGAATTGACGAGA 5 0.125 No Hit GACTATATCGTCACCCAGCAGGCCCTGGTCGCTGTGGACAATGGTACTCC 5 0.125 No Hit GCTGGAAGCTCATCAAAGAGCACTGGTTTTGCGTCAGACTCCTCCACTTT 5 0.125 No Hit ATTTGCTTATAGGCAGATGAAGATCTTTGCAGCTGTCTTGGTGGCCAGTT 5 0.125 No Hit CAACACAAAGCCAGCGCCAACCTCCCTCAGCCACTCCATTCTTCCCCAGC 5 0.125 No Hit AAGAACCAGTTATTTAGGGAGCGGATTGAGAAGCTCAAAGGCGAGGAGAA 5 0.125 No Hit GGAATTGATAGTTTGTTTCTCCTTTTTTTCAACCTTGTTTATACCTTGAA 5 0.125 No Hit CAGAAAAAGACCACAAAAAAACCCATCAACAACATCCTCTTAATCCATCA 5 0.125 No Hit GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGT 5 0.125 No Hit ATTCACTTGAGTGAGACAGAGGAGAGTGGAATTTCGTGTGTAGGGGTGAA 5 0.125 No Hit GGAGAATTGCAACGGTAATGATCGGGTGAGGGAGGATGGAGATGATGGTT 5 0.125 No Hit CTTCAACTACCCCATCTCCTTCTCAAACCTCTAACTATCCTTCTTCTCCT 5 0.125 No Hit ATGTGGCCAATTTAAAGCTCACTCACTCCCCTATGGGAATTCTAGGATTT 5 0.125 No Hit GAATGTTTCTGCATGTGTGATGCTGGATAGCGCTGAAAAATGAGTTATGT 5 0.125 No Hit AACACAAAAGCAGCTCCAGATCTCATCAAAGCAAGTCAAACGACAGGACT 5 0.125 No Hit AGCAATGTCACGGGCAAGAACCACAGCTGAACGAGGAGATCTAGCTGGTG 5 0.125 No Hit GACATTAATTAGGGCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCA 5 0.125 No Hit CTATTGCAGAAATGAGAGGCCGTTTTCTGTAGATAACTTCAGTTTTGGAG 5 0.125 No Hit TGGCCCTCCAAAGCTACGCCTTACAGGAATAGAGCTGCCCCAACATGGTT 5 0.125 No Hit TGAAGCTAGGTACTATACTGTACTTTTCGGTGTATCAAGGAGTATTGGCA 5 0.125 No Hit AAAAAACTTTGATGGAAGTCCTGAGTTCGTAGCGGGTAAAACAAAAGTTA 5 0.125 No Hit AAGAGATGGAACAGGGCAGCATGATGATTGAGAGTCCCTCTTCGGTGCAT 5 0.125 No Hit AGAGAGGAAAAGGATTAGGCCAGATCTTGTGTTGCGGTTACAGATTGAAG 5 0.125 No Hit GCTATTTCACGTGCATGGATTATTAGCTGGGTTATTGAGCTCCCTAGTGG 5 0.125 No Hit CAGCAAGTATGCACGTGGCCAACGAATGTCCTTTCTTCAGCTGCAACAAC 5 0.125 No Hit GGAACAACCTGACTTGGAAGAAGGAAGCAGTGATTCAGAAGAGGATGGAA 5 0.125 No Hit CAACAATGACAACCGTTGGGGATGGCATGGAAAATATCGGTCTTGTAAGC 5 0.125 No Hit ATACTTTCTACAGCAACAAGGAGATCTTTCTCCGTGAACTTATCAGCAAT 5 0.125 No Hit GATACAAGGAAATTTTGTGTAATTGTGGCCTTATGTTAATTCCGCCTCCT 5 0.125 No Hit GTGGAATATAACTGGTGGAACTGATTTAACATTGTTTGAGGTGAATGCTG 5 0.125 No Hit AAACAAATGCTACAAACATCTTTTTAGTAAAAAAGGGCAGAGTTCTGACA 5 0.125 No Hit GTGAAGAGGAGAGGTAAAAATAATGGCTGCAAAGCGTATCTTGAAGGAAC 5 0.125 No Hit CACAAACCCAAAATTCCCAATCAGCTTTAGATCTATTAGGATCTCGAAGC 5 0.125 No Hit TTTCTGTCTTTCAAATCCTTGTCTTTCTTACGAATCGAGAGAAGTTAAGA 5 0.125 No Hit CATAGTTATTTTTTAGACTAGATAAGATATATGAATTGTGTGAAGATGGG 5 0.125 No Hit CCGGATACTTTGTTGGAGAAAGCTAGACAGGAGCAGGAACATGTTACTGC 5 0.125 No Hit GGCTAACAAAGGCACTTTGCCTCAAGAAACCCTCACTGACATCCTCTCAA 5 0.125 No Hit ATTTTTGGATCTTTTTAAAGAGGAAATTTAGGGTTTGGTTTGTTTGTTGG 5 0.125 No Hit CCGAGGTGAATCATGAGGATTTTAATGAAGGTATCATCCAGGTGCAAGCG 5 0.125 No Hit AGTGAGATCTGCATCTCTTCCAACACAAAAATCTTTGTTTCTTTCGACAT 5 0.125 No Hit ATGAAACCCTTTGTTTTCTAACACACACCTCTACTCCTTCTTTGACTTCT 5 0.125 No Hit AATGACCTACGCTCCAATAGGCTTGATGCGTGTCCCGCCAGACCAGTCCA 5 0.125 No Hit CTGGATTTTGTGCCAGTGGCTGTGCAGCAATTGCTGATTCTGGAACCTCT 5 0.125 No Hit ATACAGAAAAACATCCAAAACCAGAACCAACAACACAGAATCAATCTGCT 5 0.125 No Hit GTGGGCCCCCCTGTTCCCACCGTGGAATCTCCTCTTGAACTCACCCCCGT 5 0.125 No Hit AACACACTAGAGAGAGCAGGAGCCGCTAGGGCTCTTTCATTTCCTTCGAG 5 0.125 No Hit GCAAGGGCAGCCTGTCTTTATCACTAGACTAGAAGGTTATAGGAGTGCTT 5 0.125 No Hit GGCTTGAGCAGATTCATTCGCCAACTAACCCTTTAATTTATCCTATTTTT 5 0.125 No Hit AATAGCAACTTTCACGATGATGCTTCTAAGTCAATTTATATTTGACAAAT 5 0.125 No Hit TCAAGTTTAAGAAAGACGAAGGGCTGATTGATGGTACAGAGTGCTCTGTT 5 0.125 No Hit GTGTGCTTTTAGTAAACACTGCCTTGTCAATTTCCATTGTCAAAGGGATA 5 0.125 No Hit ATTATTGGGGGTGTTTTCGACTTCTACTTCTTTTCCGGGCCCTCTCCTCT 5 0.125 No Hit ATGAATTCCAAGCAGGAGGACCACGCGCTGCTTTGCACTATGTAGCTACA 5 0.125 No Hit GTTTGATTTATTTAGCTCCATCCCGTGCTTCTTTCCAAAATCTAACAAAT 5 0.125 No Hit AGGAAATAGATGCAAATTCAGATGCTGCCGCCTCAGTAGCTGCACTTAGA 5 0.125 No Hit TGAGTGTGCCTCCTTCGATTTATAAGAAGGTGAAGCAAGCGCCTGAGAAC 5 0.125 No Hit GGAAATTTTGTCCTTAAACTTGAGGCCTTCTTTAATTCCTGCGTTCTAGT 5 0.125 No Hit TGCTGCTGCTTTGGACCCTTGATCCAGCTGAAAGGGATGCTGCTTTGGCT 5 0.125 No Hit TGGATCATGACAGGAACACGTGTCCACTCTGTAGGACTTCGTTTGTGCCT 5 0.125 No Hit GCCAGACTTATGAACCCAATCGACACACATTTGAGCACTTTCGTCAATGG 5 0.125 No Hit CCAAATCAGATTCTCCTGTTGAAGATACCTCTCTGACTGGTTTCAAGGGA 5 0.125 No Hit CCTTCTCTTTCTCTGAAGAACTGGACAGACTAAAACATGTGTGGAGGTGC 5 0.125 No Hit GTTGAAACCTCAAACTACCTATCTGAATATCTCTATATATTTTACCTGTC 5 0.125 No Hit CCTTGGACAGCCGTTACATGCCAGGTTGGTGACATCCTGAACGAACTTGG 5 0.125 No Hit ATTTCAGGAAAAAAAGGAAATAAGTCCTAATTATTGTTTGTAATCGCCAT 5 0.125 No Hit CTGATATCCATCAGCAGGGCTGCTGTTGTTAGCTCTTATGATAAAATGGT 5 0.125 No Hit TAAAAGGGAGATCGCTGCTTTCTTGGGCCAAACCTCTCATGAAACTACCG 5 0.125 No Hit GGTAGGGGTGGCATCTCTCCCATCGTACCCTCGAGATGATGACACTGAAG 5 0.125 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.025 0.0 0.0 0.0 0.0 64-65 0.05 0.0 0.0 0.0 0.0 66-67 0.075 0.0 0.0 0.0 0.0 68-69 0.1 0.0 0.0 0.0 0.0 70-71 0.125 0.0 0.0 0.0 0.0 72-73 0.125 0.0 0.0 0.0 0.0 74-75 0.175 0.0 0.0 0.0 0.0 76-77 0.2 0.0 0.0 0.0 0.0 78-79 0.2 0.0 0.0 0.0 0.0 80-81 0.2 0.0 0.0 0.0 0.0 82-83 0.21250000000000002 0.0 0.0 0.0 0.0 84-85 0.2875 0.0 0.0 0.0 0.0 86-87 0.325 0.0 0.0 0.0 0.0 88-89 0.36250000000000004 0.0 0.0 0.0 0.0 90-91 0.4 0.0 0.0 0.0 0.0 92-93 0.4 0.0 0.0 0.0 0.0 94-95 0.5375000000000001 0.0 0.0 0.0 0.0 96-97 0.575 0.0 0.0 0.0 0.0 98-99 0.6875 0.0 0.0 0.0 0.0 100-101 0.8374999999999999 0.0 0.0 0.0 0.0 102-103 1.1124999999999998 0.0 0.0 0.0 0.0 104-105 1.3 0.0 0.0 0.0 0.0 106-107 1.8125 0.0 0.0 0.0 0.0 108-109 2.1500000000000004 0.0 0.0 0.0 0.0 110-111 2.4125 0.0 0.0 0.0 0.0 112-113 2.775 0.0 0.0 0.0 0.0 114-115 3.1875 0.0 0.0 0.0 0.0 116-117 3.875 0.0 0.0 0.0 0.0 118-119 4.449999999999999 0.0 0.0 0.0 0.0 120-121 5.050000000000001 0.0 0.0 0.0 0.0 122-123 5.5875 0.0 0.0 0.0 0.0 124-125 6.2125 0.0 0.0 0.0 0.0 126-127 7.1375 0.0 0.0 0.0 0.0 128-129 7.637499999999999 0.0 0.0 0.0 0.0 130-131 8.0875 0.0 0.0 0.0 0.0 132-133 8.524999999999999 0.0 0.0 0.0 0.0 134-135 9.05 0.0 0.0 0.0 0.0 136-137 9.4625 0.0 0.0 0.0 0.0 138-139 10.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CAGTTTT 10 0.006830828 145.0 9 TGCCGTT 10 0.006830828 145.0 2 TCTAATT 10 0.006830828 145.0 7 ACGAGGT 10 0.006830828 145.0 145 TTCTCAC 10 0.006830828 145.0 6 CTCACCA 10 0.006830828 145.0 8 TGAAACG 10 0.006830828 145.0 8 GTGCCGT 10 0.006830828 145.0 1 CATCATC 40 0.0076550315 18.125 50-54 >>END_MODULE Read 942879 spots for SRR26075372.sra Written 942879 spots for SRR26075372.sra Read 942879 spots for SRR26075372.sra Written 942879 spots for SRR26075372.sra Read 942879 spots for SRR26075372.sra Written 942879 spots for SRR26075372.sra Read 942879 spots for SRR26075372.sra Written 942879 spots for SRR26075372.sra Read 942879 spots for SRR26075372.sra Written 942879 spots for SRR26075372.sra Read 942879 spots for SRR26075372.sra Written 942879 spots for SRR26075372.sra Read 942879 spots for SRR26075372.sra Written 942879 spots for SRR26075372.sra Read 942879 spots for SRR26075372.sra Written 942879 spots for SRR26075372.sra Read 942879 spots for SRR26075372.sra Written 942879 spots for SRR26075372.sra Read 942879 spots for SRR26075372.sra Written 942879 spots for SRR26075372.sra Read 942879 spots for SRR26075372.sra Written 942879 spots for SRR26075372.sra Read 942886 spots for SRR26075372.sra Written 942886 spots for SRR26075372.sra Read 942879 spots for SRR26075372.sra Written 942879 spots for SRR26075372.sra Read 942879 spots for SRR26075372.sra Written 942879 spots for SRR26075372.sra Read 942879 spots for SRR26075372.sra Written 942879 spots for SRR26075372.sra Read 942879 spots for SRR26075372.sra Written 942879 spots for SRR26075372.sra Read 942879 spots for SRR26075372.sra Written 942879 spots for SRR26075372.sra Read 942879 spots for SRR26075372.sra Written 942879 spots for SRR26075372.sra Read 942879 spots for SRR26075372.sra Written 942879 spots for SRR26075372.sra Read 942879 spots for SRR26075372.sra Written 942879 spots for SRR26075372.sra SRR ids: ['SRR26075372.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_e6k1fyuo SRR26075372.sra spots: 18857587 blocks: [[1, 942879], [942880, 1885758], [1885759, 2828637], [2828638, 3771516], [3771517, 4714395], [4714396, 5657274], [5657275, 6600153], [6600154, 7543032], [7543033, 8485911], [8485912, 9428790], [9428791, 10371669], [10371670, 11314548], [11314549, 12257427], [12257428, 13200306], [13200307, 14143185], [14143186, 15086064], [15086065, 16028943], [16028944, 16971822], [16971823, 17914701], [17914702, 18857587]] SRR26075372 file size 6958827 SRR26075372 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o SRR26075372 SRR26075372_1.fastq SRR26075372_2.fastq Input file: SRR26075372_1.fastq Paired file: SRR26075372_2.fastq trimmed: SRR26075372-trimmed-pair1.fastq, SRR26075372-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Tue Feb 11 23:08:17 2025 >> started Tue Feb 11 23:08:38 2025 >> done (21.269s) 18857587 read pairs processed; of these: 96 ( 0.00%) short read pairs filtered out after trimming by size control 37902 ( 0.20%) empty read pairs filtered out after trimming by size control 18819589 (99.80%) read pairs available; of these: 2759022 (14.66%) trimmed read pairs available after processing 16060567 (85.34%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 18 5 0.00% 19 13 0.00% 20 7 0.00% 21 9 0.00% 22 21 0.00% 23 16 0.00% 24 20 0.00% 25 29 0.00% 26 20 0.00% 27 48 0.00% 28 28 0.00% 29 35 0.00% 30 37 0.00% 31 27 0.00% 32 23 0.00% 33 23 0.00% 34 48 0.00% 35 41 0.00% 36 46 0.00% 37 55 0.00% 38 39 0.00% 39 68 0.00% 40 41 0.00% 41 60 0.00% 42 48 0.00% 43 78 0.00% 44 88 0.00% 45 81 0.00% 46 96 0.00% 47 83 0.00% 48 125 0.00% 49 174 0.00% 50 138 0.00% 51 156 0.00% 52 170 0.00% 53 190 0.00% 54 135 0.00% 55 243 0.00% 56 211 0.00% 57 252 0.00% 58 325 0.00% 59 311 0.00% 60 431 0.00% 61 473 0.00% 62 484 0.00% 63 535 0.00% 64 559 0.00% 65 610 0.00% 66 705 0.00% 67 867 0.00% 68 868 0.00% 69 1150 0.01% 70 1318 0.01% 71 1437 0.01% 72 1586 0.01% 73 1909 0.01% 74 2044 0.01% 75 2322 0.01% 76 2676 0.01% 77 2842 0.02% 78 3394 0.02% 79 3610 0.02% 80 3992 0.02% 81 4320 0.02% 82 5284 0.03% 83 5882 0.03% 84 6418 0.03% 85 7227 0.04% 86 7617 0.04% 87 7950 0.04% 88 8733 0.05% 89 9374 0.05% 90 10304 0.05% 91 11428 0.06% 92 12749 0.07% 93 13919 0.07% 94 14601 0.08% 95 15481 0.08% 96 16399 0.09% 97 17363 0.09% 98 18111 0.10% 99 19164 0.10% 100 19835 0.11% 101 21300 0.11% 102 22910 0.12% 103 23927 0.13% 104 26156 0.14% 105 26640 0.14% 106 28413 0.15% 107 29037 0.15% 108 30058 0.16% 109 31134 0.17% 110 31052 0.16% 111 33226 0.18% 112 33732 0.18% 113 35557 0.19% 114 37385 0.20% 115 38847 0.21% 116 39751 0.21% 117 41267 0.22% 118 42231 0.22% 119 42362 0.23% 120 43333 0.23% 121 44731 0.24% 122 44812 0.24% 123 46816 0.25% 124 48542 0.26% 125 50078 0.27% 126 51840 0.28% 127 52576 0.28% 128 53621 0.28% 129 54403 0.29% 130 54896 0.29% 131 55819 0.30% 132 56458 0.30% 133 58625 0.31% 134 59328 0.32% 135 60864 0.32% 136 61778 0.33% 137 63033 0.33% 138 64366 0.34% 139 66127 0.35% 140 65443 0.35% 141 66161 0.35% 142 68029 0.36% 143 68997 0.37% 144 71022 0.38% 145 71193 0.38% 146 71835 0.38% 147 73353 0.39% 148 75779 0.40% 149 73575 0.39% 150 76970 0.41% 151 16060567 85.34% 18819589 reads passed initial QC criterion=sequence-density sequence-density=0.31 sequence-density-rank=1 fanout-score=2.64 fanout-score-rank=30 prefix-density=0.40 prefix-fanout=2.0 sequence=CACTTGCAGCCATTCTCGGCTCCCACGACCGTCTCAGCAGCTCCCGCAAAGTGACCCTTCTCTGGTGCCACACCAAGAACCAGAGTTTCGTTGGTGATCGTCTCTGAGGAGCTCATGTCAGGGTACA criterion=fanout-score sequence-density=0.04 sequence-density-rank=33 fanout-score=52.36 fanout-score-rank=1 prefix-density=0.24 prefix-fanout=9.8 sequence=TTCTTCACAATGCCAGCCTGAACAAGGAAGGTCGATACATTCTTGCGCTGGTCACCTTGAAGTTGAATAACCTGGCCTAATTCAGGGTCCTGCACCACTGTACCATTACAGCAGAACTCTTTCTTGAGGTCCTTTAGTATCTTGTTATAGCTGAATTCTTTTTTCAAACCTTGCACAGTTGTCAAGCTTTTCCTACCATTGCGTTGCTGTATACGAATGTGCACATAATCTTTTGTCCCAGCACCAGAGTCCTCGGCATTTGCATCAGCAAAAGGATCATAAGCTGAAGGAGTTTGGGCGTCGAAATCAGACATGAAAACTTAACTGTTCAAGGAAGTCCAACAACCTGAAAGCTCAGACCTTATCCGGTGATTAGGGTTTCGACGCGTTAGATTATTTGATTGATATGAAGCGAAACCCTAAATCGGATGAGTCTAAGCTTTTCTGATG criterion=sequence-density sequence-density=0.33 sequence-density-rank=1 fanout-score=2.75 fanout-score-rank=35 prefix-density=0.35 prefix-fanout=2.5 sequence=TGGTTTTACTAG criterion=fanout-score sequence-density=0.10 sequence-density-rank=20 fanout-score=349.52 fanout-score-rank=1 prefix-density=0.98 prefix-fanout=34.0 sequence=AAGAAGAAGAAA SRR26075372 testing PE reads STAR mapping to Ensembl genome Started job on | Feb 11 23:09:43 Started mapping on | Feb 11 23:09:43 Finished on | Feb 11 23:12:43 Mapping speed, Million of reads per hour | 376.39 Number of input reads | 18819589 Average input read length | 286 UNIQUE READS: Uniquely mapped reads number | 14954283 Uniquely mapped reads % | 79.46% Average mapped length | 287.31 Number of splices: Total | 14213806 Number of splices: Annotated (sjdb) | 13882059 Number of splices: GT/AG | 13936503 Number of splices: GC/AG | 217273 Number of splices: AT/AC | 14943 Number of splices: Non-canonical | 45087 Mismatch rate per base, % | 0.43% Deletion rate per base | 0.03% Deletion average length | 3.14 Insertion rate per base | 0.02% Insertion average length | 2.61 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 373489 % of reads mapped to multiple loci | 1.98% Number of reads mapped to too many loci | 64877 % of reads mapped to too many loci | 0.34% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 17.89% % of reads unmapped: other | 0.32% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 3491817 3491817 3491817 N_multimapping 373489 373489 373489 N_noFeature 426274 14803507 512110 N_ambiguous 238816 2526 171814 UnstrandedReadsAssigned:14289193 PositiveStrandReadsAssigned:148250 NegativeStrandReadsAssigned:14270359 Dataset is classified negative stranded MeadianReadLen=151 20thPercentileLength=151 echo kmer=147 SRR26075372 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,400 [index] number of k-mers: 62,057,036 [index] number of equivalence classes: 130,681 [quant] running in paired-end mode [quant] will process pair 1: SRR26075372-trimmed-pair1.fastq SRR26075372-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 18,819,589 reads, 16,404,353 reads pseudoaligned [quant] estimated average fragment length: 211.346 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,131 rounds 52401 SRR26075372.ke.tsv 34699 SRR26075372.se.tsv 87100 total ==> SRR26075372.ke.tsv <== target_id length eff_length est_counts tpm Potri.005G200100.1.v4.1 2018 1807.65 1711 60.5458 Potri.005G024800.1.v4.1 1035 824.654 840 65.1564 Potri.004G059700.1.v4.1 961 750.66 1 0.0852131 Potri.007G009000.2.v4.1 1416 1205.65 0 0 Potri.003G141000.2.v4.1 2943 2732.65 723.7 16.9404 Potri.016G087400.1.v4.1 270 94.936 872 587.536 Potri.015G069301.1.v4.1 564 356.439 0 0 Potri.010G195200.1.v4.1 1773 1562.65 303 12.4031 Potri.012G127500.1.v4.1 977 766.66 9677 807.398 ==> SRR26075372.se.tsv <== Potri.001G166300.v4.1 0 Potri.001G448400.v4.1 18 Potri.001G233950.v4.1 0 Potri.001G122700.v4.1 80 Potri.001G212900.v4.1 7 Potri.001G182400.v4.1 0 Potri.001G256600.v4.1 0 Potri.001G040500.v4.1 0 Potri.001G416900.v4.1 1 Potri.001G452600.v4.1 352 SRR26075372 completed mapping pipeline successfully