Starting /dee2/code/volunteer_pipeline.sh SRR26075373
    current disk space = 3052351819776
    free memory = 1435780124 
SRR26075373 SRAfilesize
c7fbae756db37d37b5438d9e5e195646  SRR26075373.sra
SRR26075373.sra file validated
SRR26075373 is paired end
SRR26075373 is conventional basespace
SRR26075373 read1 length is 151 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR26075373_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	151
%GC	53
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.6905	37.0	37.0	37.0	37.0	37.0
2	36.6395	37.0	37.0	37.0	37.0	37.0
3	36.6195	37.0	37.0	37.0	37.0	37.0
4	36.674	37.0	37.0	37.0	37.0	37.0
5	36.7095	37.0	37.0	37.0	37.0	37.0
6	36.675	37.0	37.0	37.0	37.0	37.0
7	36.6825	37.0	37.0	37.0	37.0	37.0
8	36.627	37.0	37.0	37.0	37.0	37.0
9	36.6945	37.0	37.0	37.0	37.0	37.0
10-14	36.676199999999994	37.0	37.0	37.0	37.0	37.0
15-19	36.638400000000004	37.0	37.0	37.0	37.0	37.0
20-24	36.6222	37.0	37.0	37.0	37.0	37.0
25-29	36.561800000000005	37.0	37.0	37.0	37.0	37.0
30-34	36.4728	37.0	37.0	37.0	37.0	37.0
35-39	36.4357	37.0	37.0	37.0	37.0	37.0
40-44	36.0539	37.0	37.0	37.0	37.0	37.0
45-49	32.9412	37.0	32.2	37.0	19.0	37.0
50-54	34.219	37.0	34.6	37.0	24.6	37.0
55-59	30.8544	37.0	24.6	37.0	11.0	37.0
60-64	31.202999999999996	37.0	21.8	37.0	13.8	37.0
65-69	30.7622	37.0	19.0	37.0	13.8	37.0
70-74	33.7134	37.0	32.2	37.0	21.8	37.0
75-79	35.974900000000005	37.0	37.0	37.0	37.0	37.0
80-84	36.0841	37.0	37.0	37.0	37.0	37.0
85-89	36.0129	37.0	37.0	37.0	37.0	37.0
90-94	36.007600000000004	37.0	37.0	37.0	37.0	37.0
95-99	35.9848	37.0	37.0	37.0	37.0	37.0
100-104	36.0203	37.0	37.0	37.0	37.0	37.0
105-109	35.9337	37.0	37.0	37.0	37.0	37.0
110-114	35.8174	37.0	37.0	37.0	37.0	37.0
115-119	35.82340000000001	37.0	37.0	37.0	37.0	37.0
120-124	35.852799999999995	37.0	37.0	37.0	37.0	37.0
125-129	35.7218	37.0	37.0	37.0	37.0	37.0
130-134	35.6189	37.0	37.0	37.0	37.0	37.0
135-139	35.529799999999994	37.0	37.0	37.0	37.0	37.0
140-144	35.4918	37.0	37.0	37.0	37.0	37.0
145-149	35.3727	37.0	37.0	37.0	37.0	37.0
150-151	35.1695	37.0	37.0	37.0	31.0	37.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.0
1101	2	0.0
1101	3	0.0
1101	4	0.0
1101	5	0.0
1101	6	0.0
1101	7	0.0
1101	8	0.0
1101	9	0.0
1101	10-14	0.0
1101	15-19	0.0
1101	20-24	0.0
1101	25-29	0.0
1101	30-34	0.0
1101	35-39	0.0
1101	40-44	0.0
1101	45-49	0.0
1101	50-54	0.0
1101	55-59	0.0
1101	60-64	0.0
1101	65-69	0.0
1101	70-74	0.0
1101	75-79	0.0
1101	80-84	0.0
1101	85-89	0.0
1101	90-94	0.0
1101	95-99	0.0
1101	100-104	0.0
1101	105-109	0.0
1101	110-114	0.0
1101	115-119	0.0
1101	120-124	0.0
1101	125-129	0.0
1101	130-134	0.0
1101	135-139	0.0
1101	140-144	0.0
1101	145-149	0.0
1101	150-151	0.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
21	1.0
22	3.0
23	3.0
24	4.0
25	9.0
26	13.0
27	16.0
28	20.0
29	24.0
30	40.0
31	51.0
32	94.0
33	768.0
34	392.0
35	324.0
36	2088.0
37	150.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	51.151151151151154	9.534534534534535	8.65865865865866	30.655655655655657
2	14.975	36.175000000000004	23.75	25.1
3	15.4	13.950000000000001	45.0	25.650000000000002
4	16.35	18.25	20.25	45.15
5	44.275	21.975	16.975	16.775000000000002
6	41.3	25.424999999999997	18.625	14.649999999999999
7	11.35	45.2	31.5	11.95
8	13.475000000000001	45.75	22.775000000000002	18.0
9	38.775	18.25	24.75	18.224999999999998
10-14	19.3	32.265	20.27	28.165000000000003
15-19	20.865000000000002	25.945	25.724999999999998	27.465
20-24	19.99	31.380000000000003	25.374999999999996	23.255
25-29	20.19	26.640000000000004	25.695	27.474999999999998
30-34	14.665000000000001	26.450000000000003	25.35	33.535
35-39	20.495	26.875	30.285	22.345000000000002
40-44	15.120000000000001	30.94	25.755	28.185
45-49	21.81	24.415	30.240000000000002	23.535
50-54	26.115	20.26	25.424999999999997	28.199999999999996
55-59	20.32	20.330000000000002	33.97	25.380000000000003
60-64	27.334999999999997	19.775000000000002	31.95	20.94
65-69	24.13	31.130000000000003	26.224999999999998	18.515
70-74	40.745	20.135	20.544999999999998	18.575
75-79	41.365	20.330000000000002	20.47	17.835
80-84	40.93	20.200000000000003	20.755000000000003	18.115000000000002
85-89	41.695	19.689999999999998	20.225	18.39
90-94	41.74	20.855	19.89	17.515
95-99	41.415	19.935	20.76	17.89
100-104	41.870000000000005	19.985	20.53	17.615
105-109	42.02	19.865	20.200000000000003	17.915
110-114	41.925000000000004	20.39	19.725	17.96
115-119	42.07	21.01	18.94	17.98
120-124	42.265	20.919999999999998	19.470000000000002	17.345
125-129	42.14	21.38	19.45	17.03
130-134	42.26	20.035	19.27	18.435000000000002
135-139	43.035000000000004	20.695	18.68	17.59
140-144	42.8	20.255000000000003	18.925	18.02
145-149	42.3	19.875	19.165	18.66
150-151	42.5	19.1875	20.200000000000003	18.1125
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.5
5	0.5
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.5
13	0.5
14	0.0
15	0.0
16	0.5
17	2.0
18	1.5
19	0.0
20	1.5
21	2.0
22	1.0
23	1.0
24	0.5
25	0.5
26	1.0
27	3.0
28	4.0
29	10.5
30	17.5
31	21.0
32	26.5
33	29.0
34	36.0
35	46.5
36	58.0
37	83.0
38	96.0
39	91.5
40	100.0
41	132.5
42	165.0
43	174.0
44	171.5
45	196.5
46	199.5
47	176.0
48	171.0
49	163.5
50	146.5
51	119.5
52	96.0
53	93.0
54	83.0
55	52.0
56	36.0
57	22.0
58	22.5
59	23.0
60	14.5
61	15.5
62	15.0
63	11.0
64	11.5
65	62.5
66	167.5
67	244.0
68	234.5
69	163.5
70	87.5
71	44.5
72	23.5
73	11.0
74	7.5
75	4.0
76	2.5
77	1.0
78	0.0
79	0.0
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.1
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-151	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
151	4000.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	45.225
#Duplication Level	Percentage of deduplicated	Percentage of total
1	62.07849640685461	28.075
2	21.558872305140962	19.5
3	8.12603648424544	11.025
4	4.25649530127142	7.7
5	1.9900497512437811	4.5
6	0.7739082365948038	2.1
7	0.4975124378109453	1.575
8	0.2763957987838585	1.0
9	0.055279159756771695	0.22499999999999998
>10	0.22111663902708678	2.1999999999999997
>50	0.055279159756771695	1.9
>100	0.055279159756771695	5.65
>500	0.055279159756771695	14.549999999999999
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTTAACATCTCGTAT	582	14.549999999999999	TruSeq Adapter, Index 2 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTTAACATCGCGTAT	226	5.65	TruSeq Adapter, Index 2 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTTAACATCTCGTTT	76	1.9	TruSeq Adapter, Index 2 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTTAACATCGCGTTT	49	1.225	TruSeq Adapter, Index 2 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTTAACATCGCGGAT	15	0.375	TruSeq Adapter, Index 2 (97% over 37bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACCGCTTAACATCTCGTATGCC	14	0.35000000000000003	TruSeq Adapter, Index 2 (97% over 34bp)
CCATCCATCTATGATATAAAATAACATCTTCTAAAGCAACAAACAAGACC	10	0.25	No Hit
AAGAGATTTATCCTCCTGGTGACCATTTAAAGCTTCAGCTGAAATGTCTG	9	0.22499999999999998	No Hit
GCGTGTTGGAATGAAACATTAAACATAACCCAAACAAAAAATCCCCGTCT	8	0.2	No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTTAACATCTCGGAT	8	0.2	TruSeq Adapter, Index 2 (97% over 37bp)
GTTTGTTCTGCTGCCGCGGCAGGTCTTGCTTTGGTAATTGGTAGTGAGGA	8	0.2	No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTTAACATCTCGTGT	8	0.2	TruSeq Adapter, Index 2 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTTAACATCGCGGTT	8	0.2	TruSeq Adapter, Index 2 (97% over 37bp)
ACCATATCCAAATCGCCTGACTCTGCTACCACAGAAACATAACCACAACA	7	0.17500000000000002	No Hit
CAGCAGAAACCTTACGAGGTCCACCGAAACCATGAGCCAGGATACTTTCG	7	0.17500000000000002	No Hit
CCACAAGCATTCTGATCGAACCTTTTCGCCCTTCAACAGTCCCTCGATTT	7	0.17500000000000002	No Hit
CAGTTTGGTTTAAGAGCCTCTAAACCCCATTAGCTGGAGGTGAAGAATTT	7	0.17500000000000002	No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTTAACATCTCGGTT	7	0.17500000000000002	TruSeq Adapter, Index 2 (97% over 37bp)
ACCAAATTCACATATGATAGATAGAGAAGCCAAAGTTCAAACAAGGATAG	7	0.17500000000000002	No Hit
CTATTTTATCTAGAAGCACCATCAGGTCATGAAGATAAAAATACAGGATT	7	0.17500000000000002	No Hit
ATCAAAATTATCGTTAAGGAAGTCTCCGTATGCTTTATTTCGAACGTAAA	7	0.17500000000000002	No Hit
GGATGTTGTAGTCAGCCAGAGTGCGCCCATCCTCTAGCTGCTTGCCAGCA	7	0.17500000000000002	No Hit
CGCGTACACAAGACAAAGGAATTTTAGGAACCCTTTGCTGTTTATTATTA	6	0.15	No Hit
GTTAGCTCTAAAAAACCGAGCTGTTAATCAGCCAGTGCCTCCTGTCTCTC	6	0.15	No Hit
GCATCATCAATATTGGAGGCACTCATCATTCCTCTAACAAAGAGCTCTAC	6	0.15	No Hit
TAGCTTTCTAATCCCTCCATAAAATCCATCTGCCTCATTCCCCTCCTCCC	6	0.15	No Hit
GCACGCGCATCTAGGTAGGTAGGGCATGGCTCACTACTGGGTCCTCAGCC	6	0.15	No Hit
GCCACCAGCACATGGATATGTGGAACTTGGAGAGCAATAAGATCCAGGAT	6	0.15	No Hit
GAAGCATTCTGCAAATCAGCATCCTTTAATGTGCAGAACTCATCTAGTAT	6	0.15	No Hit
CGTACCCATTGAGGAATTGGGATGTAAACAACATCCCCTACATTCCCACC	6	0.15	No Hit
CGTGGCTCACCAGCAGGCTGCTTCCTTTTCTGAATGAAACTCATGTTTGT	6	0.15	No Hit
AAGATCGCCACTCAAGAGGAGGCTTGAGACTTCTTTGACTTGCTGGTTGC	6	0.15	No Hit
GACCGTGTTGCATTCATTCTACCATCTGCATACCCTCGATCACCATTCCT	6	0.15	No Hit
CCTGCAACTTGAAGTCCCTTTTTAATGAAGGTAAGCACCTTGGTTCGCTT	6	0.15	No Hit
GCAATGATATCAACCTTTAAGTGATGCACCCACTTTCCCAAGCTCTTCTA	6	0.15	No Hit
CTTTCGTGGAGGTGTGGCGATGCCTCTTGGGTTTCTTGATGCCATTTTGG	6	0.15	No Hit
CCTCTTTAATCCTGCTTCTTTGGGATCCTTAACTAAGGTCTTGATTTCAG	5	0.125	No Hit
CCGGCTTCATGTAGGCGGGTTGCAGCCTACAATCCGAACTACGAACGGCT	5	0.125	No Hit
GCACCTTCTTTGTAATAAGCAAAGACCAAACAACCATCATCATGCATGCT	5	0.125	No Hit
CCCTCTTTCTTCTCATCATCCGTCAGATCACCATAAAGGAGACTGAGATG	5	0.125	No Hit
TAGTAGTAACAGCAGAGCAGTGGTTTCATGGATAACAAGTGAAACAGCAG	5	0.125	No Hit
GTAGGCATTACCCAGATGATGGTTTCAATCACACTATACTTAACTGGGGG	5	0.125	No Hit
GTCGTAATGTGCTTTCATGGTTTGCAGAAAGCTTCTAAACCACTCATTGG	5	0.125	No Hit
AGAGGCTTTGGTCGGGTGTGTGTTGGTCTGTGAGGGGGATAACAGTGGAG	5	0.125	No Hit
CATGATAAAAATGCAATTGGAACAAAAATTAACACGCCCATCCCAGCATC	5	0.125	No Hit
CGGCGAGGTGAGTAGCTGCGGGACCGTCTGTGGCCATGACCATGACCTCG	5	0.125	No Hit
CGCTCGGCCAATTCTCGAGCAGTGCAAGTGGACAGGGGAGAGAAGACGAT	5	0.125	No Hit
CCTCCCCTTCACTTTCCTCGTCCTCCTCAATGCTTTCCTCCAATTTTGAG	5	0.125	No Hit
CTTCAACATCGTACCTCACTGTCCATCCATGAGCAACCTTTGGAAATATC	5	0.125	No Hit
GAGCCGGAGTTTTGGGTGCACTGCTGCTGGGTTTAGCTGCAGGCTCTTTC	5	0.125	No Hit
GTGAAGAATCAAGTTGTTTTAGTACACAGATTGCAGTGTGACTCTTGTAT	5	0.125	No Hit
GAATGACACACAAACATGATAAAAGTACATCAGATTATCAATCTTCTACA	5	0.125	No Hit
ATCTCATTCCTCGTCTGAACCTGAACTCTCTGAGCTTGAGCCTTTTTTCT	5	0.125	No Hit
AGACTTTTCAATTCTGGAAAATGAACAAGAATGATTAAACCTAAGTGTTC	5	0.125	No Hit
GACTGACTGATCATAGCTTGGTTGAGTTGGTGCTGGTTGAGCATAACCCT	5	0.125	No Hit
CCAGCATCATTCGCCTCTAGAGTAAGGTGGTATAGCTTACCAGCAACCAC	5	0.125	No Hit
TAGTGACCGAGCATAATCGTAATGATCCTCTTAGTGATTGTAAAGGGGTG	5	0.125	No Hit
GGTGGACTCAAAGTCAAAAATAGACTTCATCAATTTGACATAATCGCTAG	5	0.125	No Hit
TGCACGATGAAATTCTGGTGTTGCACGATGAAATTCTGGTGTTGCTTCCA	5	0.125	No Hit
GGAGTACTGACCACCTGGTTTACTATAGGCCACAATAACTACTTTGTTGG	5	0.125	No Hit
TCCGTCAACCGGCATATCTGTTTGTTTATTCCATGGGAAAGGCTTCATCG	5	0.125	No Hit
CTCTACCAACCTGTTTTGTACCCCACTGCTCGTCATAGCCCTTGTGCTCC	5	0.125	No Hit
CAGAATAAAGGTTTGGCTGGGCCTCAAAAATCCTGATAGATGATCGGCTG	5	0.125	No Hit
GTTGGAAGACGGGGGGAATTATGATATTTGTAAGGCCAAGACGAATCTTA	5	0.125	No Hit
CACACAGATTGCATCACATAGCTTTACTTTCTTACATACACAATCGATTA	5	0.125	No Hit
GGCAGAATCGTCCGAGTTCACCGATGGAGTTGCCCCCAAAAAATTCATGT	5	0.125	No Hit
TTCTCATATGGGCAAACCCAGTTTCCTTCATGAACTCCAATTTCATATAT	5	0.125	No Hit
GGGTGGAGATGAACTCAGCCAAGGCTGCCTTCAAGGCATCTGGCTGAGTT	5	0.125	No Hit
CCCTTCCTCGTATTGAGTGTCATCATTTGGATTCCCAGGCACTATCTTCT	5	0.125	No Hit
CTTCCCTTTGCTGGCGGATGAGACTCAATCGTTCCAAGTCTTTCCTAGCT	5	0.125	No Hit
CCAACTTGACCCGGACCACATAGTTAGCAATCTTTAGGACTTGCTGACCC	5	0.125	No Hit
CCTTGATTGAATTCTTTATCCTTCCATTCAAAGCCTTGCCAGCGAACCCA	5	0.125	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.125	0.0	0.0	0.0	0.0
2	0.125	0.0	0.0	0.0	0.0
3	0.125	0.0	0.0	0.0	0.0
4	0.125	0.0	0.0	0.0	0.0
5	0.125	0.0	0.0	0.0	0.0
6	0.125	0.0	0.0	0.0	0.0
7	0.125	0.0	0.0	0.0	0.0
8	0.125	0.0	0.0	0.0	0.0
9	0.125	0.0	0.0	0.0	0.0
10-11	0.125	0.0	0.0	0.0	0.0
12-13	0.125	0.0	0.0	0.0	0.0
14-15	0.125	0.0	0.0	0.0	0.0
16-17	0.125	0.0	0.0	0.0	0.0
18-19	0.125	0.0	0.0	0.0	0.0
20-21	0.125	0.0	0.0	0.0	0.0
22-23	0.125	0.0	0.0	0.0	0.0
24-25	0.125	0.0	0.0	0.0	0.0
26-27	0.125	0.0	0.0	0.0	0.0
28-29	0.125	0.0	0.0	0.0	0.0
30-31	0.125	0.0	0.0	0.0	0.0
32-33	0.125	0.0	0.0	0.0	0.0
34-35	0.125	0.0	0.0	0.0	0.0
36-37	0.125	0.0	0.0	0.0	0.0
38-39	0.125	0.0	0.0	0.0	0.0
40-41	0.125	0.0	0.0	0.0	0.0
42-43	0.2	0.0	0.0	0.0	0.0
44-45	0.2	0.0	0.0	0.0	0.0
46-47	0.2	0.0	0.0	0.0	0.0
48-49	0.2	0.0	0.0	0.0	0.0
50-51	0.2	0.0	0.0	0.0	0.0
52-53	0.2	0.0	0.0	0.0	0.0
54-55	0.2	0.0	0.0	0.0	0.0
56-57	0.225	0.0	0.0	0.0	0.0
58-59	0.225	0.0	0.0	0.0	0.0
60-61	0.2375	0.0	0.0	0.0	0.0
62-63	0.275	0.0	0.0	0.0	0.0
64-65	0.3	0.0	0.0	0.0	0.0
66-67	0.3375	0.0	0.0	0.0	0.0
68-69	0.375	0.0	0.0	0.0	0.0
70-71	0.4	0.0	0.0	0.0	0.0
72-73	0.4	0.0	0.0	0.0	0.0
74-75	0.4	0.0	0.0	0.0	0.0
76-77	0.5	0.0	0.0	0.0	0.0
78-79	0.575	0.0	0.0	0.0	0.0
80-81	0.6375	0.0	0.0	0.0	0.0
82-83	0.7	0.0	0.0	0.0	0.0
84-85	0.8125	0.0	0.0	0.0	0.0
86-87	1.0375	0.0	0.0	0.0	0.0
88-89	1.15	0.0	0.0	0.0	0.0
90-91	1.225	0.0	0.0	0.0	0.0
92-93	1.375	0.0	0.0	0.0	0.0
94-95	1.6749999999999998	0.0	0.0	0.0	0.0
96-97	2.0375	0.0	0.0	0.0	0.0
98-99	2.3	0.0	0.0	0.0	0.0
100-101	2.55	0.0	0.0	0.0	0.0
102-103	2.825	0.0	0.0	0.0	0.0
104-105	3.2125000000000004	0.0	0.0	0.0	0.0
106-107	3.825	0.0	0.0	0.0	0.0
108-109	4.3	0.0	0.0	0.0	0.0
110-111	4.75	0.0	0.0	0.0	0.0
112-113	5.0625	0.0	0.0	0.0	0.0
114-115	5.425000000000001	0.0	0.0	0.0	0.0
116-117	5.762499999999999	0.0	0.0	0.0	0.0
118-119	6.2875	0.0	0.0	0.0	0.0
120-121	6.75	0.0	0.0	0.0	0.0
122-123	7.15	0.0	0.0	0.0	0.0
124-125	7.8	0.0	0.0	0.0	0.0
126-127	8.3625	0.0	0.0	0.0	0.0
128-129	9.149999999999999	0.0	0.0	0.0	0.0
130-131	9.875	0.0	0.0	0.0	0.0
132-133	10.2625	0.0	0.0	0.0	0.0
134-135	11.2	0.0	0.0	0.0	0.0
136-137	11.8125	0.0	0.0	0.0	0.0
138-139	12.4875	0.0125	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GAGCACA	155	0.0	98.22581	9
AGAGCAC	165	0.0	92.272736	8
TCGGAAG	175	0.0	91.14286	3
ATCGGAA	175	0.0	91.14286	2
AAGAGCA	170	0.0	89.55883	7
GAAGAGC	170	0.0	89.55883	6
GGAAGAG	170	0.0	89.55883	5
GATCGGA	180	0.0	88.611115	1
CGGAAGA	175	0.0	87.0	4
TCGCGTA	30	4.189703E-5	29.000002	40-44
ACATCGC	30	4.189703E-5	29.000002	40-44
TCTCGTA	75	0.0	29.000002	40-44
TGGGTGT	30	4.189703E-5	29.000002	60-64
GCGTATG	30	4.189703E-5	29.000002	45-49
ATCGCGT	30	4.189703E-5	29.000002	40-44
CTCGTAT	75	0.0	29.000002	40-44
CGCGTAT	30	4.189703E-5	29.000002	40-44
TAAGGGG	30	4.189703E-5	29.000002	65-69
AACATCG	30	4.189703E-5	29.000002	35-39
CATCGCG	30	4.189703E-5	29.000002	40-44
>>END_MODULE
SRR26075373 read2 length is 151 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR26075373_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	151
%GC	57
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.212	37.0	37.0	37.0	37.0	37.0
2	36.282	37.0	37.0	37.0	37.0	37.0
3	36.4005	37.0	37.0	37.0	37.0	37.0
4	36.2565	37.0	37.0	37.0	37.0	37.0
5	36.333	37.0	37.0	37.0	37.0	37.0
6	36.252	37.0	37.0	37.0	37.0	37.0
7	36.0385	37.0	37.0	37.0	37.0	37.0
8	35.6295	37.0	37.0	37.0	37.0	37.0
9	35.73	37.0	37.0	37.0	37.0	37.0
10-14	35.154	37.0	37.0	37.0	25.0	37.0
15-19	34.742999999999995	37.0	37.0	37.0	25.0	37.0
20-24	34.0968	37.0	37.0	37.0	25.0	37.0
25-29	32.5377	37.0	29.8	37.0	16.6	37.0
30-34	31.7892	37.0	25.0	37.0	11.0	37.0
35-39	31.397700000000004	37.0	25.0	37.0	11.0	37.0
40-44	30.2977	37.0	25.0	37.0	11.0	37.0
45-49	29.7406	37.0	16.6	37.0	11.0	37.0
50-54	29.162899999999997	37.0	11.0	37.0	11.0	37.0
55-59	29.762099999999997	37.0	19.4	37.0	11.0	37.0
60-64	30.651799999999998	37.0	25.0	37.0	11.0	37.0
65-69	29.9914	37.0	22.2	37.0	11.0	37.0
70-74	29.3024	37.0	11.0	37.0	11.0	37.0
75-79	29.112299999999998	37.0	11.0	37.0	11.0	37.0
80-84	29.543	37.0	16.6	37.0	11.0	37.0
85-89	30.575499999999998	37.0	25.0	37.0	11.0	37.0
90-94	31.8608	37.0	29.8	37.0	11.0	37.0
95-99	33.193999999999996	37.0	37.0	37.0	16.6	37.0
100-104	33.949	37.0	37.0	37.0	25.0	37.0
105-109	34.3701	37.0	37.0	37.0	25.0	37.0
110-114	34.737100000000005	37.0	37.0	37.0	25.0	37.0
115-119	34.7787	37.0	37.0	37.0	25.0	37.0
120-124	34.8412	37.0	37.0	37.0	25.0	37.0
125-129	34.94179999999999	37.0	37.0	37.0	25.0	37.0
130-134	34.8842	37.0	37.0	37.0	25.0	37.0
135-139	34.7496	37.0	37.0	37.0	25.0	37.0
140-144	34.754400000000004	37.0	37.0	37.0	25.0	37.0
145-149	34.5909	37.0	37.0	37.0	25.0	37.0
150-151	34.4105	37.0	37.0	37.0	25.0	37.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.0
1101	2	0.0
1101	3	0.0
1101	4	0.0
1101	5	0.0
1101	6	0.0
1101	7	0.0
1101	8	0.0
1101	9	0.0
1101	10-14	0.0
1101	15-19	0.0
1101	20-24	0.0
1101	25-29	0.0
1101	30-34	0.0
1101	35-39	0.0
1101	40-44	0.0
1101	45-49	0.0
1101	50-54	0.0
1101	55-59	0.0
1101	60-64	0.0
1101	65-69	0.0
1101	70-74	0.0
1101	75-79	0.0
1101	80-84	0.0
1101	85-89	0.0
1101	90-94	0.0
1101	95-99	0.0
1101	100-104	0.0
1101	105-109	0.0
1101	110-114	0.0
1101	115-119	0.0
1101	120-124	0.0
1101	125-129	0.0
1101	130-134	0.0
1101	135-139	0.0
1101	140-144	0.0
1101	145-149	0.0
1101	150-151	0.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	1.0
13	7.0
14	12.0
15	17.0
16	20.0
17	24.0
18	21.0
19	19.0
20	22.0
21	29.0
22	68.0
23	80.0
24	126.0
25	183.0
26	220.0
27	192.0
28	133.0
29	32.0
30	15.0
31	25.0
32	38.0
33	69.0
34	168.0
35	540.0
36	1779.0
37	160.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	53.075	15.675	11.65	19.6
2	49.475	17.4	19.175	13.950000000000001
3	44.15	20.65	21.45	13.750000000000002
4	47.225	21.099999999999998	17.5	14.174999999999999
5	47.675	24.525	15.299999999999999	12.5
6	44.375	27.450000000000003	15.8	12.375
7	43.775	16.675	26.125	13.425
8	45.324999999999996	18.025	18.0	18.65
9	43.775	20.974999999999998	19.6	15.65
10-14	45.565	21.17	17.854999999999997	15.409999999999998
15-19	45.1	20.59	19.235	15.075
20-24	45.235	20.96	19.11	14.695
25-29	45.625	20.805	18.645	14.924999999999999
30-34	44.379999999999995	21.47	19.009999999999998	15.14
35-39	45.824999999999996	20.165	18.83	15.18
40-44	44.065	20.825	19.8	15.310000000000002
45-49	42.504999999999995	21.355	20.985	15.155
50-54	23.855	20.615	39.93	15.6
55-59	38.345	22.03	24.435000000000002	15.190000000000001
60-64	44.505	21.235	19.125	15.135000000000002
65-69	43.55	21.255	19.220000000000002	15.975
70-74	31.169999999999998	31.259999999999998	22.54	15.03
75-79	26.745	35.23	22.689999999999998	15.334999999999999
80-84	38.895	24.085	21.58	15.440000000000001
85-89	43.085	21.43	20.075000000000003	15.409999999999998
90-94	44.04	21.4	19.365	15.195
95-99	44.64	22.005	18.55	14.804999999999998
100-104	44.535000000000004	21.7	18.745	15.02
105-109	45.735	21.22	18.14	14.905
110-114	44.79	21.715	19.07	14.424999999999999
115-119	44.76	21.21	18.83	15.2
120-124	45.33	21.154999999999998	19.54	13.975000000000001
125-129	45.225	21.38	18.990000000000002	14.405000000000001
130-134	45.145	22.355	18.655	13.844999999999999
135-139	46.45	21.15	18.815	13.584999999999999
140-144	46.045	20.49	19.405	14.06
145-149	47.25	20.785	18.565	13.4
150-151	47.1875	20.8875	18.8375	13.087499999999999
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	1.0
6	1.0
7	0.0
8	0.5
9	0.5
10	0.0
11	1.0
12	1.5
13	1.0
14	1.5
15	1.0
16	1.5
17	1.5
18	1.5
19	3.5
20	2.5
21	1.5
22	2.5
23	2.5
24	2.0
25	1.5
26	2.0
27	2.0
28	2.0
29	3.5
30	4.0
31	9.0
32	13.0
33	13.0
34	20.5
35	34.5
36	40.0
37	52.5
38	75.0
39	97.5
40	130.0
41	157.5
42	180.5
43	181.5
44	185.0
45	208.0
46	219.5
47	199.0
48	165.0
49	157.5
50	128.0
51	103.0
52	107.5
53	89.5
54	65.0
55	56.0
56	38.5
57	19.5
58	17.0
59	17.5
60	16.0
61	13.5
62	13.0
63	11.5
64	7.0
65	4.5
66	3.5
67	5.5
68	6.0
69	5.0
70	4.0
71	3.0
72	5.5
73	6.0
74	5.5
75	5.5
76	5.0
77	6.0
78	9.5
79	18.0
80	25.5
81	28.5
82	39.5
83	50.0
84	51.0
85	67.5
86	84.0
87	89.0
88	94.0
89	88.0
90	85.5
91	75.5
92	61.5
93	51.5
94	34.0
95	21.5
96	12.0
97	9.0
98	7.5
99	2.5
100	23.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-151	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
151	4000.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	49.425000000000004
#Duplication Level	Percentage of deduplicated	Percentage of total
1	66.3631765300961	32.800000000000004
2	18.563480020232674	18.35
3	7.435508345978755	11.025
4	3.743045017703591	7.3999999999999995
5	1.8209408194233687	4.5
6	0.9104704097116844	2.7
7	0.4552352048558422	1.575
8	0.15174506828528073	0.6
9	0.10116337885685382	0.44999999999999996
>10	0.30349013657056145	2.725
>50	0.05058168942842691	1.275
>100	0.10116337885685382	16.6
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGT	477	11.924999999999999	No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG	187	4.675	No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGT	51	1.275	No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTT	27	0.675	No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGT	25	0.625	No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGT	20	0.5	No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGGT	17	0.42500000000000004	No Hit
ATTTGCTAGGAGGGAAGAACGATTAGCCATGGGACCTGGGGCAGAAAAGC	10	0.25	No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGGGGGGGGGGGGGGGT	10	0.25	No Hit
GAAGATGCATTTGAGGCACCAAAAACAGAGGAGAAAGATGAAGAAGCTGG	9	0.22499999999999998	No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGGGT	9	0.22499999999999998	No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGA	8	0.2	No Hit
CAGGGAAGCTATTATGTGTACAATGACATATTCAGGTTGAACTATGCTTG	8	0.2	No Hit
GCCCAAGTCACTACCCAAAAATGATGCACAACTCTCAGCAACACAATGCT	8	0.2	No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGC	7	0.17500000000000002	No Hit
GGTTTTTGTACTTGTCAAAGATCTGTTGAACAAATAATTCAAAAGCACTG	7	0.17500000000000002	No Hit
CTCTGACACCATCGATAATGTCAAGGCAAAAATCCAGGACAAAGAGGGCA	7	0.17500000000000002	No Hit
GTTTGTGCAACCTCTTAATTCGACAGGGTACCGAAGAAGAATGGCATCAC	7	0.17500000000000002	No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGGGGGGGGGGGGGGGGGT	7	0.17500000000000002	No Hit
CAGGGGTGTTTCGAATACAGTGTATGCTTGGTATGGCGCGCCTGCGAAGG	7	0.17500000000000002	No Hit
CCTGGTATAATGCTGCCACTGAAGCGCTCAAGGATATGAAGTTAAGAAAA	7	0.17500000000000002	No Hit
TATGGACCGTGCCTTGGGATGTCTCGGCTTGCACGTTGGGAGCGTGCTCA	7	0.17500000000000002	No Hit
CTGGGTTGGCCCATTCATTGGAGCTGCTCTTGCCGCTGTCTACCACCAGA	7	0.17500000000000002	No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGG	6	0.15	No Hit
GCGACGATTTGGATTTAGCTATCAGAAGTTTGAATGAGCTTCGTTTAGCA	6	0.15	No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGG	6	0.15	No Hit
ATGGAATCGAGCCTCAGTTATGGGCAAGATATGATTTTGGTGTTGAGCGG	6	0.15	No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGGGGGGGGT	6	0.15	No Hit
GGGGTCATGGTGGATGTATGGTGGGGGATCATAGAAGCCAAAGGCCCTAA	6	0.15	No Hit
GATTTACTCCCTGGCGATCACATTGAGATTCAGTGGAGAAGAAACAAAGA	6	0.15	No Hit
CCTGAGTTCTGTAACATGGTGAGAAGAATCTACATATGCTCAAGTCAGGA	6	0.15	No Hit
GAATCAGGCACAGAAGCAGAGACGTCGTACTTATCGGGCGCATGGAAGAA	6	0.15	No Hit
TGAGAATGGCTGCAAGTGTGGATCAAACTGCACCTGTGATCCATGCTCCT	6	0.15	No Hit
CGTTTCTAGATGCAGATGAATGGATATCACATCCCATGTCTCTTGCTGGG	6	0.15	No Hit
CTCTCATGAAACTACCGGAGGGTGGCAAACTGCACCTGATGGTGCATACG	6	0.15	No Hit
ATCTGGTTTGCATGTCTATACTCCTGGTGATTTTGTGGAGTCTGATCAGC	6	0.15	No Hit
CTGGTGGAAGTGGTTCTCTTATCATCAGCGATTCCAGTGATTATGATGTT	6	0.15	No Hit
AGCGAATTAGGGTTTGGCCTAATCGCCTCTCTTTCTGCCCCAGAGCCGCA	6	0.15	No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTTT	6	0.15	No Hit
GTACATTGAACATACTACATAGCAATGGAACTGCAAAGGGTTTGACAGTG	6	0.15	No Hit
CCTATGGAACAAGATGAAGAAGATTCTGAAACGGATAGGAGTTCAAATTT	6	0.15	No Hit
CTTTGAATAGTAAAGGTGTATCTGCAATTGGGGCTGTGGGCTTTTGTTGG	5	0.125	No Hit
ATGCTTACTTGCTTACACATCCAGGGATGCCGACAGTTTTCTACGATCAC	5	0.125	No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGGGGT	5	0.125	No Hit
AGGAAAGTGAGGATGAAGACATGGGATTCAGCTTGTTTGATTAGGAGCTC	5	0.125	No Hit
CCAACCTCATAAGTTTGTCAGGGAGTTTCCCCAGCAGCGAATGACTTTCA	5	0.125	No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTG	5	0.125	No Hit
AGACGATCGAATCAGAAACTTGTATTTGACTGTGTGAATGCAGCACTTGT	5	0.125	No Hit
CCATCTGCACAACAAGGTTATCCTGAGCAGCAAGCTGCAAACAATGCAGG	5	0.125	No Hit
GGAATCTGAGGAGGAATCCGAAGAAGATGATCCTGATCAAAAGCGGAAAG	5	0.125	No Hit
TCTTGAAGATGAAGCTTGGTGGTGTTCATGATTGTAAGGGTTCTCAAAAC	5	0.125	No Hit
AAGACATTCGTCAGGAAAATGCATGCTGATCAAGATTACTGCCCAGACCC	5	0.125	No Hit
AGGCATTACACACAGTTCGAAAATTTTGTTGTTTGGATTAATCTGTTGAG	5	0.125	No Hit
TGGCTGCAAGTGCGGATCAAACTGCACCTGCGATCCATGCAATTGCAAAT	5	0.125	No Hit
CTTTTGTTCCGCCCAAGCAAGAATCATGGGTTAACACGGTCATCACCATG	5	0.125	No Hit
AAACAAGAGAGGTGGAGATATAGGAGAGCATAACCATGTTAGTCCCATAT	5	0.125	No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGGGGGT	5	0.125	No Hit
CTTCACCTATAGCCCAAAAAGATCTACCAGTTTCCAGCTATTGATGCGAT	5	0.125	No Hit
CCTCCGTGAAGAAGTTCGGGTTCAATCAGATCCAGCAGGGCGCTTGGTTA	5	0.125	No Hit
TTTTTCTTTCAGTTTCTCTACCAAGATCCCTTCAAGTCACCATATGTTCT	5	0.125	No Hit
TTCTCTCCCAGGGGTGATTTGGGAGATGATCAGGAATTCCAATTTGATTT	5	0.125	No Hit
ATACCGTGGACAGGCTTGTTAAGTCAGTTAAGGAGTTGAAGAACATGGGG	5	0.125	No Hit
ATAGTGGATTCCATTTTGTCAACCAGGAAAATGTTTTCAAGGTCTGTGAT	5	0.125	No Hit
AGGTAGTTTTCGACAGGTAGCTGTGGCAGATGACGCTCATCAAAGTTTTG	5	0.125	No Hit
GGAGTGCCATCTGGTGACAGATCCTCGAACTAGAGAATCTCGTGGATTTG	5	0.125	No Hit
GAGAAACGGGAGGAAGGTGGGGATGACGTCAAGTCAGCATGGCCTTTATA	5	0.125	No Hit
GGCGAAAGAAGAAGAATACAGGTGATGCACGAGGATCGTCAAATCTGCCA	5	0.125	No Hit
AGCACATCCACCTCCAAGTTCACAATAACAAAAGAGTATATAGCAAAGCC	5	0.125	No Hit
GCAGAATTTTGTCCAATCAACCTTCAATGCCCTCACTCCACAAAATGTTA	5	0.125	No Hit
CGTCACAAAGCTCATACACATTTGTCATGACCCCACCTTGTCGTAGCTTG	5	0.125	No Hit
TGACAAGAAGCAGTTTCTTACACAGATTAAGAAATTTATCAAGAATCTGT	5	0.125	No Hit
TTTTTTTTTTTTGAAGGCAGAGGCTCCACTTGTACTTGTAATTATTATGT	5	0.125	No Hit
AATGAAGTTGGTAGGAGAGAGATTTAGTGAGAACAGTTGTCCAAGGATGG	5	0.125	No Hit
GATCATTCTCTAGCTAGCTTCCACAGCCAGAGTAAAAACACAGAGAGCGC	5	0.125	No Hit
CGAACACGGGCTGGCACTCGGGGTGGTTTGGTATCTGAAGAAGGACCCAT	5	0.125	No Hit
GAATAAGACAGACGAGGAATCTGAAGCCCTCCACAGGCAGTTCCTTGATA	5	0.125	No Hit
GAACACACAGGCAGAAATTGATCAGACCATTGAGGACTACCACTATAGCA	5	0.125	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.125	0.0	0.0	0.0	0.0
2	0.125	0.0	0.0	0.0	0.0
3	0.125	0.0	0.0	0.0	0.0
4	0.125	0.0	0.0	0.0	0.0
5	0.125	0.0	0.0	0.0	0.0
6	0.125	0.0	0.0	0.0	0.0
7	0.125	0.0	0.0	0.0	0.0
8	0.125	0.0	0.0	0.0	0.0
9	0.125	0.0	0.0	0.0	0.0
10-11	0.125	0.0	0.0	0.0	0.0
12-13	0.125	0.0	0.0	0.0	0.0
14-15	0.125	0.0	0.0	0.0	0.0
16-17	0.125	0.0	0.0	0.0	0.0
18-19	0.125	0.0	0.0	0.0	0.0
20-21	0.125	0.0	0.0	0.0	0.0
22-23	0.125	0.0	0.0	0.0	0.0
24-25	0.125	0.0	0.0	0.0	0.0
26-27	0.125	0.0	0.0	0.0	0.0
28-29	0.125	0.0	0.0	0.0	0.0
30-31	0.125	0.0	0.0	0.0	0.0
32-33	0.125	0.0	0.0	0.0	0.0
34-35	0.125	0.0	0.0	0.0	0.0
36-37	0.125	0.0	0.0	0.0	0.0
38-39	0.125	0.0	0.0	0.0	0.0
40-41	0.125	0.0	0.0	0.0	0.0
42-43	0.125	0.0	0.0	0.0	0.0
44-45	0.125	0.0	0.0	0.0	0.0
46-47	0.125	0.0	0.0	0.0	0.0
48-49	0.125	0.0	0.0	0.0	0.0
50-51	0.125	0.0	0.0	0.0	0.0
52-53	0.125	0.0	0.0	0.0	0.0
54-55	0.125	0.0	0.0	0.0	0.0
56-57	0.125	0.0	0.0	0.0	0.0
58-59	0.125	0.0	0.0	0.0	0.0
60-61	0.125	0.0	0.0	0.0	0.0
62-63	0.125	0.0	0.0	0.0	0.0
64-65	0.15	0.0	0.0	0.0	0.0
66-67	0.16249999999999998	0.0	0.0	0.0	0.0
68-69	0.2	0.0	0.0	0.0	0.0
70-71	0.225	0.0	0.0	0.0	0.0
72-73	0.225	0.0	0.0	0.0	0.0
74-75	0.225	0.0	0.0	0.0	0.0
76-77	0.325	0.0	0.0	0.0	0.0
78-79	0.4	0.0	0.0	0.0	0.0
80-81	0.4625	0.0	0.0	0.0	0.0
82-83	0.5249999999999999	0.0	0.0	0.0	0.0
84-85	0.6625	0.0	0.0	0.0	0.0
86-87	0.9125	0.0	0.0	0.0	0.0
88-89	1.025	0.0	0.0	0.0	0.0
90-91	1.1	0.0	0.0	0.0	0.0
92-93	1.25	0.0	0.0	0.0	0.0
94-95	1.5499999999999998	0.0	0.0	0.0	0.0
96-97	1.9375	0.0	0.0	0.0	0.0
98-99	2.2	0.0	0.0	0.0	0.0
100-101	2.475	0.0	0.0	0.0	0.0
102-103	2.75	0.0	0.0	0.0	0.0
104-105	3.1375	0.0	0.0	0.0	0.0
106-107	3.775	0.0	0.0	0.0	0.0
108-109	4.2625	0.0	0.0	0.0	0.0
110-111	4.725	0.0	0.0	0.0	0.0
112-113	5.0375	0.0	0.0	0.0	0.0
114-115	5.4125	0.0	0.0	0.0	0.0
116-117	5.762499999999999	0.0	0.0	0.0	0.0
118-119	6.2875	0.0	0.0	0.0	0.0
120-121	6.775	0.0	0.0	0.0	0.0
122-123	7.137499999999999	0.0	0.0	0.0	0.0
124-125	7.7375	0.0	0.0	0.0	0.0
126-127	8.3125	0.0	0.0	0.0	0.0
128-129	9.0875	0.0	0.0	0.0	0.0
130-131	9.85	0.0	0.0	0.0	0.0
132-133	10.2875	0.0	0.0	0.0	0.0
134-135	11.175	0.0	0.0	0.0	0.0
136-137	11.787500000000001	0.0	0.0	0.0	0.0
138-139	12.475	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGTGGA	10	0.006830828	145.0	2
GCGTCGT	40	0.005621335	54.375	145
GGGTTTT	30	4.189703E-5	29.000002	45-49
GGGGTTT	30	4.189703E-5	29.000002	45-49
GGGGTTG	30	4.189703E-5	29.000002	45-49
TTTTTGG	25	4.977651E-4	29.0	50-54
GGTTTTT	20	0.00593511	29.0	45-49
TTGTGGG	25	4.977651E-4	29.0	50-54
GGGTGTT	20	0.00593511	29.0	45-49
GGGGGTT	65	4.1836756E-11	26.76923	45-49
GGGGGGA	45	8.383813E-7	25.777777	65-69
TTTGGGG	45	8.383813E-7	25.777777	50-54
TTTTGGG	45	2.4877938E-5	22.555553	50-54
TTGGGGG	65	8.8818524E-7	20.076923	50-54
AGGGGGG	40	0.0076550315	18.125	75-79
TGGGGGG	175	7.013041E-7	10.771429	55-59
GGGGGGT	175	7.013041E-7	10.771429	40-44
AAAAAAA	375	0.0014487104	5.4133334	75-79
>>END_MODULE
Read 635584 spots for SRR26075373.sra
Written 635584 spots for SRR26075373.sra
Read 635584 spots for SRR26075373.sra
Written 635584 spots for SRR26075373.sra
Read 635584 spots for SRR26075373.sra
Written 635584 spots for SRR26075373.sra
Read 635584 spots for SRR26075373.sra
Written 635584 spots for SRR26075373.sra
Read 635584 spots for SRR26075373.sra
Written 635584 spots for SRR26075373.sra
Read 635584 spots for SRR26075373.sra
Written 635584 spots for SRR26075373.sra
Read 635584 spots for SRR26075373.sra
Written 635584 spots for SRR26075373.sra
Read 635584 spots for SRR26075373.sra
Written 635584 spots for SRR26075373.sra
Read 635584 spots for SRR26075373.sra
Written 635584 spots for SRR26075373.sra
Read 635584 spots for SRR26075373.sra
Written 635584 spots for SRR26075373.sra
Read 635584 spots for SRR26075373.sra
Written 635584 spots for SRR26075373.sra
Read 635584 spots for SRR26075373.sra
Written 635584 spots for SRR26075373.sra
Read 635590 spots for SRR26075373.sra
Written 635590 spots for SRR26075373.sra
Read 635584 spots for SRR26075373.sra
Written 635584 spots for SRR26075373.sra
Read 635584 spots for SRR26075373.sra
Written 635584 spots for SRR26075373.sra
Read 635584 spots for SRR26075373.sra
Written 635584 spots for SRR26075373.sra
Read 635584 spots for SRR26075373.sra
Written 635584 spots for SRR26075373.sra
Read 635584 spots for SRR26075373.sra
Written 635584 spots for SRR26075373.sra
Read 635584 spots for SRR26075373.sra
Written 635584 spots for SRR26075373.sra
Read 635584 spots for SRR26075373.sra
Written 635584 spots for SRR26075373.sra
SRR ids: ['SRR26075373.sra']
extra args: ['--split-files', '--defline-qual', '+']
tempdir: /tmp/pfd_jnrvjhq_
SRR26075373.sra spots: 12711686
blocks: [[1, 635584], [635585, 1271168], [1271169, 1906752], [1906753, 2542336], [2542337, 3177920], [3177921, 3813504], [3813505, 4449088], [4449089, 5084672], [5084673, 5720256], [5720257, 6355840], [6355841, 6991424], [6991425, 7627008], [7627009, 8262592], [8262593, 8898176], [8898177, 9533760], [9533761, 10169344], [10169345, 10804928], [10804929, 11440512], [11440513, 12076096], [12076097, 12711686]]
SRR26075373 file size 4687331
SRR26075373 completed basic pipeline successfully
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	skewer -f sanger -l 18 -q 10 -k inf -t 20 -o SRR26075373 SRR26075373_1.fastq SRR26075373_2.fastq
Input file:	SRR26075373_1.fastq
Paired file:	SRR26075373_2.fastq
trimmed:	SRR26075373-trimmed-pair1.fastq, SRR26075373-trimmed-pair2.fastq

Parameters used:
-- 3' end adapter sequence (-x):	AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC
-- paired 3' end adapter sequence (-y):	AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- end quality threshold (-q):		10
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ 
-- number of concurrent threads (-t):	20
Tue Feb 11 23:17:44 2025 >> started

Tue Feb 11 23:17:59 2025 >> done (14.599s)
12711686 read pairs processed; of these:
     363 ( 0.00%) short read pairs filtered out after trimming by size control
 2951295 (23.22%) empty read pairs filtered out after trimming by size control
 9760028 (76.78%) read pairs available; of these:
 2096942 (21.48%) trimmed read pairs available after processing
 7663086 (78.52%) untrimmed read pairs available after processing

Length distribution of reads after trimming:
length	count	percentage
 18	      8	  0.00%
 19	      7	  0.00%
 20	      6	  0.00%
 21	     11	  0.00%
 22	      8	  0.00%
 23	      8	  0.00%
 24	     12	  0.00%
 25	     25	  0.00%
 26	     17	  0.00%
 27	     15	  0.00%
 28	     23	  0.00%
 29	     27	  0.00%
 30	     27	  0.00%
 31	     36	  0.00%
 32	     38	  0.00%
 33	     33	  0.00%
 34	     27	  0.00%
 35	     44	  0.00%
 36	     41	  0.00%
 37	     97	  0.00%
 38	    188	  0.00%
 39	     75	  0.00%
 40	    167	  0.00%
 41	    101	  0.00%
 42	    107	  0.00%
 43	     91	  0.00%
 44	     92	  0.00%
 45	    109	  0.00%
 46	    121	  0.00%
 47	    155	  0.00%
 48	    129	  0.00%
 49	    217	  0.00%
 50	    206	  0.00%
 51	    267	  0.00%
 52	    490	  0.01%
 53	    294	  0.00%
 54	    438	  0.00%
 55	    776	  0.01%
 56	    388	  0.00%
 57	    593	  0.01%
 58	   1167	  0.01%
 59	    779	  0.01%
 60	    902	  0.01%
 61	    734	  0.01%
 62	    853	  0.01%
 63	   1376	  0.01%
 64	   1041	  0.01%
 65	   1213	  0.01%
 66	   1109	  0.01%
 67	   1196	  0.01%
 68	   1283	  0.01%
 69	   1476	  0.02%
 70	   1564	  0.02%
 71	   1936	  0.02%
 72	   2180	  0.02%
 73	   2438	  0.02%
 74	   2647	  0.03%
 75	   3028	  0.03%
 76	   3338	  0.03%
 77	   3457	  0.04%
 78	   3663	  0.04%
 79	   3960	  0.04%
 80	   4786	  0.05%
 81	   5106	  0.05%
 82	   5979	  0.06%
 83	   6554	  0.07%
 84	   7203	  0.07%
 85	   7585	  0.08%
 86	   8131	  0.08%
 87	   8399	  0.09%
 88	   9237	  0.09%
 89	   9705	  0.10%
 90	  10408	  0.11%
 91	  11798	  0.12%
 92	  12861	  0.13%
 93	  13983	  0.14%
 94	  14334	  0.15%
 95	  15271	  0.16%
 96	  15935	  0.16%
 97	  16521	  0.17%
 98	  17173	  0.18%
 99	  17395	  0.18%
100	  17944	  0.18%
101	  19347	  0.20%
102	  20634	  0.21%
103	  22361	  0.23%
104	  23115	  0.24%
105	  23444	  0.24%
106	  24167	  0.25%
107	  24334	  0.25%
108	  25191	  0.26%
109	  25595	  0.26%
110	  25658	  0.26%
111	  27218	  0.28%
112	  27943	  0.29%
113	  28525	  0.29%
114	  30076	  0.31%
115	  30852	  0.32%
116	  31200	  0.32%
117	  31473	  0.32%
118	  32468	  0.33%
119	  32517	  0.33%
120	  33088	  0.34%
121	  33711	  0.35%
122	  34531	  0.35%
123	  36307	  0.37%
124	  36933	  0.38%
125	  36978	  0.38%
126	  38704	  0.40%
127	  38404	  0.39%
128	  38889	  0.40%
129	  39338	  0.40%
130	  39991	  0.41%
131	  39771	  0.41%
132	  41134	  0.42%
133	  41317	  0.42%
134	  41297	  0.42%
135	  43425	  0.44%
136	  42908	  0.44%
137	  43760	  0.45%
138	  44472	  0.46%
139	  45630	  0.47%
140	  44716	  0.46%
141	  44958	  0.46%
142	  46168	  0.47%
143	  45969	  0.47%
144	  47009	  0.48%
145	  47256	  0.48%
146	  47471	  0.49%
147	  47901	  0.49%
148	  48248	  0.49%
149	  48287	  0.49%
150	  49091	  0.50%
151	7663086	 78.52%
9760028 reads passed initial QC


criterion=sequence-density
sequence-density=1.29
sequence-density-rank=1
fanout-score=23.96
fanout-score-rank=5
prefix-density=0.90
prefix-fanout=24.0
sequence=GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTTAACATCTCGTATGCCGTCTTCTGCTTGAAAAT


criterion=fanout-score
sequence-density=0.03
sequence-density-rank=32
fanout-score=365.38
fanout-score-rank=1
prefix-density=0.63
prefix-fanout=15.7
sequence=CCACCACCACCTCCACCACTTCCGCGGGATTGAGCTTCGTTCACGGTGATGTTACGGCCATCGAGGTCCTGACCGTTCATTCCATCAATAGCATCTCTCATTGCCTTCTCATTGCCGAAGGTCACAAATCCAAAACCACGAGATCTTCCGGTTTCACGGTCATTTATAATCTTCGAATCGATGATTTCACCGTACTGGCTAAAAGCCTCTTGGAGGACTTGGTCAGTGGTGGCCCAAGCGAGGCCGCCAACAAAGCACCTGTACTCAACCTCGGCAGACATTGCTAAACCCTAGAAAATTATAGAGAGGAAGAGTAGTGGTCTTTTGGGA


criterion=sequence-density
sequence-density=0.38
sequence-density-rank=1
fanout-score=2.76
fanout-score-rank=32
prefix-density=0.39
prefix-fanout=2.7
sequence=ATGTACCCTGAC


criterion=fanout-score
sequence-density=0.01
sequence-density-rank=36
fanout-score=72.27
fanout-score-rank=1
prefix-density=0.14
prefix-fanout=4.1
sequence=CAAAGCAGTTGCATTTATCTAAAGTATTCTCACTTTACTTAACACTTGAGCTACATAGAATGTCTACAGTCAATTTGGCAGCACTGCTGTTGCTTGGGCTGCTGCTGGTTATGCCACAGCAGTCCATGCAAGCGAGTTTGATAGACCCCATTGCTGAAATCGAGAGAAGCAACTGCAAAATCGCACACCTTCGCTTAGGGCTTGTTTTTACGTCTGATAACAACGAAAGGGCTCTGCAAGACTCTGGACTCTATAGCCCTGACAGTGAAGACTCTTCTGTTGACATTGCGGGCAGAAGGTTCCATAGCGGGACACTAAACGGTTCTTCTATTGTATATGTCAAGACAGGGAGTCATTCAGTAAATATGGCAACAACTTTGCAAATCCTT
SRR26075373 testing PE reads STAR mapping to Ensembl genome
                                 Started job on |	Feb 11 23:18:42
                             Started mapping on |	Feb 11 23:18:42
                                    Finished on |	Feb 11 23:20:41
       Mapping speed, Million of reads per hour |	295.26

                          Number of input reads |	9760028
                      Average input read length |	290
                                    UNIQUE READS:
                   Uniquely mapped reads number |	8221907
                        Uniquely mapped reads % |	84.24%
                          Average mapped length |	288.05
                       Number of splices: Total |	7299573
            Number of splices: Annotated (sjdb) |	7088192
                       Number of splices: GT/AG |	7158796
                       Number of splices: GC/AG |	106534
                       Number of splices: AT/AC |	7333
               Number of splices: Non-canonical |	26910
                      Mismatch rate per base, % |	0.41%
                         Deletion rate per base |	0.04%
                        Deletion average length |	3.42
                        Insertion rate per base |	0.02%
                       Insertion average length |	2.44
                             MULTI-MAPPING READS:
        Number of reads mapped to multiple loci |	276444
             % of reads mapped to multiple loci |	2.83%
        Number of reads mapped to too many loci |	42906
             % of reads mapped to too many loci |	0.44%
                                  UNMAPPED READS:
       % of reads unmapped: too many mismatches |	0.00%
                 % of reads unmapped: too short |	10.50%
                     % of reads unmapped: other |	1.98%
                                  CHIMERIC READS:
                       Number of chimeric reads |	0
                            % of chimeric reads |	0.00%
N_unmapped	1261677	1261677	1261677
N_multimapping	276444	276444	276444
N_noFeature	233383	8103939	301029
N_ambiguous	99732	735	48880
UnstrandedReadsAssigned:7888792 PositiveStrandReadsAssigned:117233 NegativeStrandReadsAssigned:7871998
Dataset is classified negative stranded
MeadianReadLen=151 20thPercentileLength=147 echo kmer=143
SRR26075373 Starting Kallisto paired end mapping to ensembl reference transcriptome

[quant] fragment length distribution will be estimated from the data
[index] k-mer length: 31
[index] number of targets: 52,400
[index] number of k-mers: 62,057,036
[index] number of equivalence classes: 130,681
[quant] running in paired-end mode
[quant] will process pair 1: SRR26075373-trimmed-pair1.fastq
                             SRR26075373-trimmed-pair2.fastq
[quant] finding pseudoalignments for the reads ... done
[quant] processed 9,760,028 reads, 8,119,664 reads pseudoaligned
[quant] estimated average fragment length: 197.141
[   em] quantifying the abundances ... done
[   em] the Expectation-Maximization algorithm ran for 1,258 rounds

  52401 SRR26075373.ke.tsv
  34699 SRR26075373.se.tsv
  87100 total
==> SRR26075373.ke.tsv <==
target_id	length	eff_length	est_counts	tpm
Potri.005G200100.1.v4.1	2018	1821.86	874	44.8828
Potri.005G024800.1.v4.1	1035	838.859	804	89.6706
Potri.004G059700.1.v4.1	961	764.859	3	0.366963
Potri.007G009000.2.v4.1	1416	1219.86	0	0
Potri.003G141000.2.v4.1	2943	2746.86	287	9.77526
Potri.016G087400.1.v4.1	270	96.499	931.577	903.19
Potri.015G069301.1.v4.1	564	368.327	0	0
Potri.010G195200.1.v4.1	1773	1576.86	212	12.5784
Potri.012G127500.1.v4.1	977	780.859	11349	1359.78

==> SRR26075373.se.tsv <==
Potri.001G166300.v4.1	0
Potri.001G448400.v4.1	20
Potri.001G233950.v4.1	0
Potri.001G122700.v4.1	202
Potri.001G212900.v4.1	0
Potri.001G182400.v4.1	0
Potri.001G256600.v4.1	0
Potri.001G040500.v4.1	5
Potri.001G416900.v4.1	0
Potri.001G452600.v4.1	422
SRR26075373 completed mapping pipeline successfully