Starting /dee2/code/volunteer_pipeline.sh SRR26075374
    current disk space = 3052380360704
    free memory = 1472697980 
SRR26075374 SRAfilesize
1ebf6df6b89476d73e78114030c44942  SRR26075374.sra
SRR26075374.sra file validated
SRR26075374 is paired end
SRR26075374 is conventional basespace
SRR26075374 read1 length is 151 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR26075374_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	151
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.664	37.0	37.0	37.0	37.0	37.0
2	36.5925	37.0	37.0	37.0	37.0	37.0
3	36.7475	37.0	37.0	37.0	37.0	37.0
4	36.7895	37.0	37.0	37.0	37.0	37.0
5	36.729	37.0	37.0	37.0	37.0	37.0
6	36.624	37.0	37.0	37.0	37.0	37.0
7	36.5885	37.0	37.0	37.0	37.0	37.0
8	36.6665	37.0	37.0	37.0	37.0	37.0
9	36.6335	37.0	37.0	37.0	37.0	37.0
10-14	36.6543	37.0	37.0	37.0	37.0	37.0
15-19	36.6326	37.0	37.0	37.0	37.0	37.0
20-24	36.590199999999996	37.0	37.0	37.0	37.0	37.0
25-29	36.520900000000005	37.0	37.0	37.0	37.0	37.0
30-34	36.4456	37.0	37.0	37.0	37.0	37.0
35-39	36.4428	37.0	37.0	37.0	37.0	37.0
40-44	36.4136	37.0	37.0	37.0	37.0	37.0
45-49	36.2958	37.0	37.0	37.0	37.0	37.0
50-54	36.334	37.0	37.0	37.0	37.0	37.0
55-59	36.202600000000004	37.0	37.0	37.0	37.0	37.0
60-64	36.1651	37.0	37.0	37.0	37.0	37.0
65-69	36.061	37.0	37.0	37.0	37.0	37.0
70-74	36.1438	37.0	37.0	37.0	37.0	37.0
75-79	36.1038	37.0	37.0	37.0	37.0	37.0
80-84	36.0888	37.0	37.0	37.0	37.0	37.0
85-89	35.964400000000005	37.0	37.0	37.0	37.0	37.0
90-94	35.958999999999996	37.0	37.0	37.0	37.0	37.0
95-99	35.967	37.0	37.0	37.0	37.0	37.0
100-104	35.9714	37.0	37.0	37.0	37.0	37.0
105-109	35.8498	37.0	37.0	37.0	37.0	37.0
110-114	35.73309999999999	37.0	37.0	37.0	37.0	37.0
115-119	35.5893	37.0	37.0	37.0	37.0	37.0
120-124	35.5827	37.0	37.0	37.0	37.0	37.0
125-129	35.5199	37.0	37.0	37.0	37.0	37.0
130-134	35.3863	37.0	37.0	37.0	37.0	37.0
135-139	35.1448	37.0	37.0	37.0	32.2	37.0
140-144	35.0222	37.0	37.0	37.0	25.0	37.0
145-149	34.9621	37.0	37.0	37.0	25.0	37.0
150-151	34.718	37.0	37.0	37.0	25.0	37.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.0
1101	2	0.0
1101	3	0.0
1101	4	0.0
1101	5	0.0
1101	6	0.0
1101	7	0.0
1101	8	0.0
1101	9	0.0
1101	10-14	0.0
1101	15-19	0.0
1101	20-24	0.0
1101	25-29	0.0
1101	30-34	0.0
1101	35-39	0.0
1101	40-44	0.0
1101	45-49	0.0
1101	50-54	0.0
1101	55-59	0.0
1101	60-64	0.0
1101	65-69	0.0
1101	70-74	0.0
1101	75-79	0.0
1101	80-84	0.0
1101	85-89	0.0
1101	90-94	0.0
1101	95-99	0.0
1101	100-104	0.0
1101	105-109	0.0
1101	110-114	0.0
1101	115-119	0.0
1101	120-124	0.0
1101	125-129	0.0
1101	130-134	0.0
1101	135-139	0.0
1101	140-144	0.0
1101	145-149	0.0
1101	150-151	0.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
21	1.0
22	0.0
23	2.0
24	6.0
25	7.0
26	9.0
27	18.0
28	19.0
29	24.0
30	28.0
31	35.0
32	47.0
33	110.0
34	167.0
35	472.0
36	2840.0
37	215.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	37.230846269404104	14.321482223335003	8.087130696044067	40.36054081121683
2	18.7	14.85	33.925	32.525
3	18.975	16.625	27.425	36.975
4	21.9	24.15	24.349999999999998	29.599999999999998
5	24.474999999999998	27.6	26.1	21.825
6	22.575	32.7	23.724999999999998	21.0
7	17.075000000000003	28.825	38.525	15.575
8	17.7	26.25	31.8	24.25
9	17.150000000000002	25.074999999999996	33.324999999999996	24.45
10-14	20.555	28.54	27.58	23.325000000000003
15-19	19.830000000000002	27.815	27.54	24.815
20-24	20.305	27.529999999999998	28.110000000000003	24.055
25-29	20.07	27.76	28.175	23.995
30-34	20.549999999999997	27.675	27.33	24.445
35-39	19.75	27.55	28.1	24.6
40-44	19.885	27.36	27.235	25.52
45-49	19.535	27.99	27.939999999999998	24.535
50-54	21.265	28.194999999999997	27.08	23.46
55-59	20.064999999999998	27.534999999999997	28.52	23.880000000000003
60-64	20.885	26.75	27.939999999999998	24.425
65-69	20.835	27.63	27.794999999999998	23.74
70-74	21.075	27.765	27.37	23.79
75-79	22.009999999999998	27.150000000000002	26.93	23.91
80-84	21.235	28.32	26.555	23.89
85-89	20.625	27.41	27.744999999999997	24.22
90-94	21.295	27.139999999999997	27.92	23.645
95-99	21.23	26.565	27.46	24.745
100-104	20.89	27.125	27.889999999999997	24.095
105-109	20.724999999999998	26.825	27.54	24.91
110-114	20.995	27.355	26.640000000000004	25.009999999999998
115-119	20.849999999999998	27.755000000000003	26.779999999999998	24.615000000000002
120-124	21.59	27.43	27.045	23.935000000000002
125-129	21.97	27.465	26.52	24.044999999999998
130-134	20.95	27.169999999999998	27.02	24.86
135-139	21.485000000000003	27.215	26.640000000000004	24.66
140-144	22.919999999999998	27.794999999999998	24.89	24.395
145-149	22.040000000000003	27.66	26.06	24.240000000000002
150-151	22.7125	28.475	24.5375	24.275
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.5
18	0.5
19	1.0
20	1.0
21	0.0
22	0.0
23	1.0
24	2.5
25	3.5
26	3.0
27	4.0
28	5.5
29	8.5
30	12.0
31	22.5
32	33.5
33	36.0
34	39.5
35	49.5
36	60.5
37	79.0
38	116.0
39	141.5
40	172.0
41	206.0
42	257.0
43	275.5
44	231.0
45	245.0
46	303.5
47	290.0
48	216.5
49	178.5
50	156.5
51	139.0
52	124.5
53	109.5
54	97.0
55	85.5
56	76.5
57	47.0
58	31.5
59	32.0
60	25.0
61	14.0
62	7.5
63	5.5
64	5.0
65	10.0
66	9.5
67	6.5
68	8.0
69	5.5
70	2.5
71	2.0
72	1.0
73	1.5
74	1.5
75	0.0
76	0.0
77	0.0
78	0.0
79	0.0
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.15
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-151	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
151	4000.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	62.050000000000004
#Duplication Level	Percentage of deduplicated	Percentage of total
1	64.14182111200645	39.800000000000004
2	21.958098307816275	27.250000000000004
3	7.856567284448025	14.625
4	3.7066881547139405	9.2
5	0.9266720386784851	2.875
6	0.4834810636583401	1.7999999999999998
7	0.5640612409347301	2.45
8	0.16116035455278002	0.8
9	0.12087026591458502	0.675
>10	0.08058017727639001	0.525
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCAGAACTTGTTTCCACTGCTCGGAACAAATATCAAGTTAAAGCTACCCG	11	0.27499999999999997	No Hit
CCTCGATAACGGGCTTCTCCAATATCCCTCCTCCTCCTCCTCCTCCTTTG	10	0.25	No Hit
CTGTCTTAGAAGAACCAGCAGCAAATGTTTTTAATTTGTGCAACACCGCA	9	0.22499999999999998	No Hit
GCCATGTACACATGCTGAGGGAAACAAACCACTGTATCTAATCATCACTC	9	0.22499999999999998	No Hit
CACATGTTTATTCAATGCCGGCGAGTTGATTCGACAACCTAATACAGCAG	9	0.22499999999999998	No Hit
CTTGTTTGAGATGATAGCTGTATCATTAACTTAAGAAAGCGAGGAACAAA	8	0.2	No Hit
CACGCAAATTACGTAAGCAGTCATTATCAGCTTGGGATAATACCAAGTTA	8	0.2	No Hit
CTCCTAACAATGCTTTAACAACTGCTTCTTGGCTAGGTTCGGTGGATTGT	8	0.2	No Hit
CCCACTGTCTGCTTTGCTCATGACTGTCGATATTTATGTAGAGCTTCACA	8	0.2	No Hit
CATGGCTGGTGCTGGCTTTGAAGAAACAGTGTTGATCTCCTCCACTTTGC	7	0.17500000000000002	No Hit
CCCATCAACAAGCGATATATCATAAAAATCTAAGTTACCAAATTGATTTA	7	0.17500000000000002	No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTACGTTATCTCGTTT	7	0.17500000000000002	TruSeq Adapter, Index 6 (97% over 37bp)
AGGGTCATCATGATGTACTTTCTTCATCCTATATGCCTCCATATCCTCAG	7	0.17500000000000002	No Hit
GGAGGATTCTTATCCTCATAGAGACCTGGATCCTAGCTTGCCCCATCATT	7	0.17500000000000002	No Hit
CGGGAACCAAACAAGCTTGACTAGAAACTAAAGTCTTAATATCATAACCA	7	0.17500000000000002	No Hit
CTCCGTAGCTGAAGATTGCATATATACAAAAAGAAAACACAAAATAAAGG	7	0.17500000000000002	No Hit
TCACGAGTTTATAAGGAATATATTATATGCTGTGCAGTAACTGCACCAAA	7	0.17500000000000002	No Hit
GTCAACAAACCCTTCCTTGCGCTGACCTTGTGCGCCGTCCCTGTGATCCT	7	0.17500000000000002	No Hit
CCAGCACCACCGCCATGAGGAACAGGCCCTATCATTGTGGGTACCACTGG	7	0.17500000000000002	No Hit
GGGTGGAGGAGATGGCCCGAAGTCGAGATTTGGGTAGACGCCTGGTGGCC	7	0.17500000000000002	No Hit
CCTTCATCTGGTCAACAAAACCTTCCTTGCGCTGACCTTGTGCGCCGTCC	7	0.17500000000000002	No Hit
GCATGGCATGGCGTGCTTGAAGAGAGGCTGTGATACTTTTGAATGCTTTC	7	0.17500000000000002	No Hit
GGGCATGGTGTTTCAGGTTTTGGTGGCTTCACGACTGGTGGTTTTGGTGT	7	0.17500000000000002	No Hit
ATTTATTTCAAGCTCTCAACTCGCTTCAACTAAACATGCAATACCTATAT	6	0.15	No Hit
GCAGGTGATGAGCCGCCACCACTGATGTCCATCAACCACTTTCTGTACAT	6	0.15	No Hit
CTTAGGCTTTGGCTTGGGAATTCTGTTAACACTGGCAACCTTTTCTTGCA	6	0.15	No Hit
CCGGCCAGTAACCCAACAGATAACATTATAACTTGTCTTCTCTCGATGAC	6	0.15	No Hit
TACCTTTGTGGGTCCCTTCGCCGAGCTTTCTCTCCAGGATGCGCGTACGG	6	0.15	No Hit
CCCAACAGATGTCATCTCCGTTCACTGTCTTGCGATTCTCCTTATGACAC	6	0.15	No Hit
CTTGGCCGTTCATTCCATCAATCGCATCTCTCATTGCCTTCTCGTTGTTG	6	0.15	No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTACGTTATCTCGTAT	6	0.15	TruSeq Adapter, Index 6 (97% over 37bp)
GTTGCAATGGCACCCCCAGCAATGGCATCAATTACAACATTGTCTTTACT	6	0.15	No Hit
TACCGCTACTGATCGAGGGCATTAGGGCCAGTACAATAACTGACCAAATG	6	0.15	No Hit
GCACAACATGACTAAGCAACTATTAAATGAGTCCTTCACTTTGACCAGAA	6	0.15	No Hit
GTTCTAACTCTTCAATAATCTTTTTACCTGCAACAACCTCATCTATACTG	6	0.15	No Hit
CTCCATTGTTTCTTTCCTGGTGTTCAGATTCCTGATTTTCGCCAGTCGAT	5	0.125	No Hit
CTCACCAAGAACATTTTGAGTACGAGTAGCCCTGATGATGATCTCAGTGC	5	0.125	No Hit
CCCTCACACAAATCGGCTATCTCCACAGGAGCACTCGGATCCTGTCCTGC	5	0.125	No Hit
CTGGAAAGTGGTGCAGGACTGCGCTTGACCGCCCTCGACACCACCATCTC	5	0.125	No Hit
GTTCGCTTCTGTATGGGGGCCTCTTGTATCGCTCTCCTGCTTCGACGTAG	5	0.125	No Hit
CTCCTCCTTTCAGCTGCTCGATCCCTGTAGGAAGTCTGTGGTTGCTCCTT	5	0.125	No Hit
GTGGAAGTGGACTTGTCGAAGACAATGAATGCAAAGCTCCCACTAAGGTG	5	0.125	No Hit
TGCCATTGAATTCCATCAAATACTTTATGAGTTCTATTGTTAGACTAGCA	5	0.125	No Hit
TCTAAGTTTGTGCTTCCCTTCAATCTCATAACAGCATCAACAGCCAGTTT	5	0.125	No Hit
GTACTGTGCTCTGGCTCGCTTGCAGCTGTGGCGGAGATACTGCTTGCCAT	5	0.125	No Hit
GGAGGACTTGGTGCACTTGCCAGACTGATCTCGAACAAGATCTCGAAGAT	5	0.125	No Hit
AAGGGCATCGTATCTCATTGATCAAGACCAATTAGATTTAGAAACATAAG	5	0.125	No Hit
AACCAGTCACCAGTCCAACCGCCACCAGAGGGCATATCATGCCACCAATC	5	0.125	No Hit
ATTTCTCTTTCTTTACCTTCTCTTTTGAAGGATATTCATTTAAAGCTCTG	5	0.125	No Hit
GCCACCTTCTTCCAATCCCTCCTGAGGTTGTCCAGCAATCCAGCTTTGCA	5	0.125	No Hit
GCCCAGGTACTTTGACTGGCTGGAAACGTCTTTCTAAAGCAGGGTCCTTC	5	0.125	No Hit
GCCCCCTTAATCAGAATAACATCATCATCAGCAATTCGCTCCTCTACAAC	5	0.125	No Hit
CTCCTCCTTCACCACGGACGCCACTGCCACCGCCACCCCCAGGGATCTTG	5	0.125	No Hit
CCGCAATAATCAACCCATAAATACCCAACACACCAGCCATAACAACTGGA	5	0.125	No Hit
GTTGGCTTAATAATATCACCAGATATTACAGGGTTTTTATCTGTTTGGAG	5	0.125	No Hit
CTCATGGTTATGTTTTCCTTCATATGTTGTAATAAAAGCTTTTGGATCGT	5	0.125	No Hit
GGCTCGGTCAGGTTGAAGAACAGGGTGGATGGCTCATTCAGCGTGTTGAG	5	0.125	No Hit
GTTCTGTGATCTACTAGCGCCAATCTATATTACTCACAATATCCTAAAAA	5	0.125	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	0.0	0.0
16-17	0.0	0.0	0.0	0.0	0.0
18-19	0.0	0.0	0.0	0.0	0.0
20-21	0.0	0.0	0.0	0.0	0.0
22-23	0.0	0.0	0.0	0.0	0.0
24-25	0.0	0.0	0.0	0.0	0.0
26-27	0.0	0.0	0.0	0.0	0.0
28-29	0.0	0.0	0.0	0.0	0.0
30-31	0.0	0.0	0.0	0.0	0.0
32-33	0.0	0.0	0.0	0.0	0.0
34-35	0.0	0.0	0.0	0.0	0.0
36-37	0.0	0.0	0.0	0.0	0.0
38-39	0.0	0.0	0.0	0.0	0.0
40-41	0.0	0.0	0.0	0.0	0.0
42-43	0.0	0.0	0.0	0.0	0.0
44-45	0.0	0.0	0.0	0.0	0.0
46-47	0.0	0.0	0.0	0.0	0.0
48-49	0.0	0.0	0.0	0.0	0.0
50-51	0.0	0.0	0.0	0.0	0.0
52-53	0.0	0.0	0.0	0.0	0.0
54-55	0.0	0.0	0.0	0.0	0.0
56-57	0.0	0.0	0.0	0.0	0.0
58-59	0.0	0.0	0.0	0.0	0.0
60-61	0.037500000000000006	0.0	0.0	0.0	0.0
62-63	0.1	0.0	0.0	0.0	0.0
64-65	0.1	0.0	0.0	0.0	0.0
66-67	0.1	0.0	0.0	0.0	0.0
68-69	0.1	0.0	0.0	0.0	0.0
70-71	0.1	0.0	0.0	0.0	0.0
72-73	0.1	0.0	0.0	0.0	0.0
74-75	0.1125	0.0	0.0	0.0	0.0
76-77	0.125	0.0	0.0	0.0	0.0
78-79	0.15	0.0	0.0	0.0	0.0
80-81	0.175	0.0	0.0	0.0	0.0
82-83	0.2	0.0	0.0	0.0	0.0
84-85	0.2875	0.0	0.0	0.0	0.0
86-87	0.4875	0.0	0.0	0.0	0.0
88-89	0.6375	0.0	0.0	0.0	0.0
90-91	0.8625	0.0	0.0	0.0	0.0
92-93	1.15	0.0	0.0	0.0	0.0
94-95	1.2625	0.0	0.0	0.0	0.0
96-97	1.4500000000000002	0.0	0.0	0.0	0.0
98-99	1.5625	0.0	0.0	0.0	0.0
100-101	1.875	0.0	0.0	0.0	0.0
102-103	2.0875	0.0	0.0	0.0	0.0
104-105	2.4	0.0	0.0	0.0	0.0
106-107	2.85	0.0	0.0	0.0	0.0
108-109	3.2249999999999996	0.0	0.0	0.0	0.0
110-111	3.5625	0.0	0.0	0.0	0.0
112-113	3.95	0.0	0.0	0.0	0.0
114-115	4.612500000000001	0.0	0.0	0.0	0.0
116-117	5.050000000000001	0.0	0.0	0.0	0.0
118-119	5.7375	0.0	0.0	0.0	0.0
120-121	6.637499999999999	0.0	0.0	0.0	0.0
122-123	7.15	0.0	0.0	0.0	0.0
124-125	7.6	0.0	0.0	0.0	0.0
126-127	8.05	0.0	0.0	0.0	0.0
128-129	8.7625	0.0	0.0	0.0	0.0
130-131	9.5375	0.0	0.0	0.0	0.0
132-133	10.1375	0.0	0.0	0.0	0.0
134-135	10.8625	0.0	0.0	0.0	0.0
136-137	11.575	0.0	0.0	0.0	0.0
138-139	12.3625	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGACATG	10	0.006830828	145.0	5
CCCATCA	10	0.006830828	145.0	1
CCATCAA	10	0.006830828	145.0	2
ACAAGCG	10	0.006830828	145.0	8
CAAGCGA	10	0.006830828	145.0	9
>>END_MODULE
SRR26075374 read2 length is 151 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR26075374_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	151
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.463	37.0	37.0	37.0	37.0	37.0
2	36.359	37.0	37.0	37.0	37.0	37.0
3	36.249	37.0	37.0	37.0	37.0	37.0
4	36.29	37.0	37.0	37.0	37.0	37.0
5	36.2235	37.0	37.0	37.0	37.0	37.0
6	36.243	37.0	37.0	37.0	37.0	37.0
7	36.3065	37.0	37.0	37.0	37.0	37.0
8	36.321	37.0	37.0	37.0	37.0	37.0
9	36.176	37.0	37.0	37.0	37.0	37.0
10-14	36.3127	37.0	37.0	37.0	37.0	37.0
15-19	36.239999999999995	37.0	37.0	37.0	37.0	37.0
20-24	36.1476	37.0	37.0	37.0	37.0	37.0
25-29	36.029700000000005	37.0	37.0	37.0	37.0	37.0
30-34	35.9409	37.0	37.0	37.0	37.0	37.0
35-39	35.932100000000005	37.0	37.0	37.0	37.0	37.0
40-44	35.864599999999996	37.0	37.0	37.0	37.0	37.0
45-49	35.70309999999999	37.0	37.0	37.0	37.0	37.0
50-54	35.611599999999996	37.0	37.0	37.0	37.0	37.0
55-59	35.6623	37.0	37.0	37.0	37.0	37.0
60-64	35.6888	37.0	37.0	37.0	37.0	37.0
65-69	35.6423	37.0	37.0	37.0	37.0	37.0
70-74	35.5551	37.0	37.0	37.0	37.0	37.0
75-79	35.4777	37.0	37.0	37.0	37.0	37.0
80-84	35.5345	37.0	37.0	37.0	37.0	37.0
85-89	35.432100000000005	37.0	37.0	37.0	37.0	37.0
90-94	35.428900000000006	37.0	37.0	37.0	37.0	37.0
95-99	35.5156	37.0	37.0	37.0	37.0	37.0
100-104	35.4285	37.0	37.0	37.0	37.0	37.0
105-109	35.374	37.0	37.0	37.0	37.0	37.0
110-114	35.307399999999994	37.0	37.0	37.0	37.0	37.0
115-119	35.3775	37.0	37.0	37.0	37.0	37.0
120-124	35.197599999999994	37.0	37.0	37.0	32.2	37.0
125-129	35.11750000000001	37.0	37.0	37.0	29.8	37.0
130-134	35.112100000000005	37.0	37.0	37.0	32.2	37.0
135-139	34.9328	37.0	37.0	37.0	25.0	37.0
140-144	34.9816	37.0	37.0	37.0	27.4	37.0
145-149	34.93729999999999	37.0	37.0	37.0	25.0	37.0
150-151	34.688	37.0	37.0	37.0	25.0	37.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.0
1101	2	0.0
1101	3	0.0
1101	4	0.0
1101	5	0.0
1101	6	0.0
1101	7	0.0
1101	8	0.0
1101	9	0.0
1101	10-14	0.0
1101	15-19	0.0
1101	20-24	0.0
1101	25-29	0.0
1101	30-34	0.0
1101	35-39	0.0
1101	40-44	0.0
1101	45-49	0.0
1101	50-54	0.0
1101	55-59	0.0
1101	60-64	0.0
1101	65-69	0.0
1101	70-74	0.0
1101	75-79	0.0
1101	80-84	0.0
1101	85-89	0.0
1101	90-94	0.0
1101	95-99	0.0
1101	100-104	0.0
1101	105-109	0.0
1101	110-114	0.0
1101	115-119	0.0
1101	120-124	0.0
1101	125-129	0.0
1101	130-134	0.0
1101	135-139	0.0
1101	140-144	0.0
1101	145-149	0.0
1101	150-151	0.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	1.0
13	3.0
14	3.0
15	9.0
16	10.0
17	13.0
18	7.0
19	6.0
20	9.0
21	15.0
22	10.0
23	10.0
24	8.0
25	11.0
26	12.0
27	13.0
28	21.0
29	15.0
30	29.0
31	25.0
32	27.0
33	74.0
34	170.0
35	674.0
36	2598.0
37	227.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	40.35	21.0	12.525	26.125
2	29.7	26.674999999999997	26.325	17.299999999999997
3	22.95	28.375	31.025000000000002	17.65
4	24.825	33.4	22.1	19.675
5	25.7	34.175	21.025	19.1
6	22.575	38.725	22.0	16.7
7	22.925	21.525	37.3	18.25
8	23.625	25.75	27.1	23.525
9	25.775	25.025	26.875	22.325
10-14	26.38	28.199999999999996	24.94	20.48
15-19	24.805	28.804999999999996	25.955000000000002	20.435
20-24	25.72	27.72	26.634999999999998	19.925
25-29	25.615	27.49	26.115	20.78
30-34	25.405	27.860000000000003	26.07	20.665
35-39	25.674999999999997	27.24	26.584999999999997	20.5
40-44	25.8	27.91	26.200000000000003	20.09
45-49	25.564999999999998	27.560000000000002	26.135	20.74
50-54	24.335	27.845	27.76	20.06
55-59	25.245	27.900000000000002	26.985	19.869999999999997
60-64	24.565	27.555000000000003	26.91	20.97
65-69	25.169999999999998	28.720000000000002	25.885	20.225
70-74	24.95	27.860000000000003	27.01	20.18
75-79	25.169999999999998	27.805000000000003	26.815	20.21
80-84	24.91	27.79	26.44	20.86
85-89	25.585	27.295	26.445	20.674999999999997
90-94	25.145	29.099999999999998	26.22	19.535
95-99	25.369999999999997	28.425	26.090000000000003	20.115
100-104	25.115	28.470000000000002	25.985000000000003	20.43
105-109	25.55	28.42	26.125	19.905
110-114	25.180000000000003	28.845	25.814999999999998	20.16
115-119	25.525	29.330000000000002	25.335	19.81
120-124	26.41	28.410000000000004	25.240000000000002	19.939999999999998
125-129	26.255	28.904999999999998	25.595000000000002	19.245
130-134	26.56	28.044999999999998	25.535000000000004	19.86
135-139	26.650000000000002	27.91	26.47	18.970000000000002
140-144	27.85	28.449999999999996	24.349999999999998	19.35
145-149	27.839999999999996	28.205000000000002	25.569999999999997	18.385
150-151	26.875	27.737499999999997	27.187499999999996	18.2
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.5
7	2.0
8	2.0
9	1.0
10	1.0
11	0.5
12	1.0
13	1.5
14	1.0
15	0.5
16	0.0
17	1.0
18	1.5
19	0.5
20	0.5
21	1.5
22	3.0
23	2.5
24	2.5
25	4.5
26	7.5
27	7.0
28	3.0
29	6.0
30	12.0
31	14.0
32	18.0
33	23.5
34	26.0
35	39.5
36	56.0
37	79.0
38	119.5
39	144.5
40	154.5
41	178.5
42	225.5
43	257.5
44	282.5
45	304.5
46	282.0
47	264.0
48	251.0
49	218.5
50	188.5
51	163.0
52	127.5
53	90.0
54	75.5
55	62.0
56	49.0
57	35.5
58	25.0
59	29.0
60	18.0
61	11.5
62	15.0
63	10.5
64	7.0
65	4.5
66	3.0
67	3.0
68	3.0
69	2.0
70	2.0
71	2.0
72	1.0
73	0.0
74	0.0
75	2.5
76	3.0
77	3.0
78	3.0
79	0.5
80	0.5
81	2.5
82	4.0
83	4.5
84	5.0
85	4.0
86	2.5
87	2.5
88	3.5
89	3.0
90	2.0
91	2.0
92	1.5
93	0.5
94	0.5
95	0.5
96	0.0
97	0.0
98	0.5
99	1.5
100	9.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-151	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
151	4000.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	63.349999999999994
#Duplication Level	Percentage of deduplicated	Percentage of total
1	66.33780584056828	42.025
2	20.441988950276244	25.900000000000002
3	7.576953433307025	14.399999999999999
4	3.67008681925809	9.3
5	0.7892659826361484	2.5
6	0.3946329913180742	1.5
7	0.35516969218626676	1.575
8	0.15785319652722968	0.8
9	0.23677979479084454	1.35
>10	0.03946329913180742	0.65
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG	26	0.65	No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGT	9	0.22499999999999998	No Hit
AAGAAATACACAATGGCAGGAATCATGCACAAGATTGAGGAGACTCTGAA	9	0.22499999999999998	No Hit
CTGCACCGTCAAAGTACACAGGAATGTTGCAAGCTACAAAGGATATATTT	9	0.22499999999999998	No Hit
ATTGTCCTGCCAGTGCAAAGTGGAGTCTCTCTTGTTGGAACTGGTTTCTT	9	0.22499999999999998	No Hit
GGGAGAAGGGGGCGACAATGGAGACTGCGATTTGTGGGAGAATAGCTCTC	9	0.22499999999999998	No Hit
CTTGATGCGGGTGGTGTTATATCTGTTTTTGAGAATAAACTTGGAGATTG	9	0.22499999999999998	No Hit
GTAAGGACTTGGGTGCAGCAAATTCCAACCACTCTGTTGTGTCGGACATG	8	0.2	No Hit
CTTTGCCCCCATCGATGAACCTTTTATTAGGAGAACCAGGGACTGGAGGA	8	0.2	No Hit
TGAAATTTTTGATGGAACGTAACAGTAGTAGTTCTCTTCATGGTCAAGTG	8	0.2	No Hit
GGTAATAGATGATTATTTCCTTGGCGCAAGAGTTTCTTGGATTAACGAGG	8	0.2	No Hit
GAGACACGATGGCTAAGTTTGCTGTGGCTAATCTCGTGATCCTTCTTTTG	7	0.17500000000000002	No Hit
CTGCTATGTGGATCTTCATCTCTTTGTTGCCAAGAGATATCTGGATTTTC	7	0.17500000000000002	No Hit
CGAGGTTCCAGAAGATTTGGTTTTGGATGAAAAGTTACTTGCTGAAGCGC	7	0.17500000000000002	No Hit
TGGACGTTGGTCAAGGACGTGGGGAGAGGAACATTTTGGAAACGGGAAAG	7	0.17500000000000002	No Hit
GAGAAAGGGCTGAACTCTGAAGGTGAAAGAGAGGCAGATTGCAGAGATTG	7	0.17500000000000002	No Hit
GAGAAAAAAGAAGAAAGAAATACACAATGGCAGGAATCATGCACAAGATT	7	0.17500000000000002	No Hit
CTGATAAAAAAAATATTCTCTCCTCGCTCTCTCAAACTTCCAACACGAAG	7	0.17500000000000002	No Hit
GGTAAGGGTCGTTGCCAAACTGGGGATTGCACCGGTGGCCTAGAGTGCAA	7	0.17500000000000002	No Hit
GTGCCTGCAATTTGATCTTTACTGATTAAGAGGTGGTCAAAGTGGCTCTA	7	0.17500000000000002	No Hit
ATTACAGTCTATGAAATAGATAAAGAGACTGAGAATGTTAAAGTTACAAT	6	0.15	No Hit
CGCATGCTATGTGCAAACCATGATGATCGGAGAACATCCTCATCACCATC	6	0.15	No Hit
CCAAAATCTATAGAGTTAGCTCGAAAGTATCCTGGTGAGCTGCAACAGAT	6	0.15	No Hit
TCATCTTCAGTGCTCTGGCTCGAAGATCGATCGATTTGTTCATTTTCTCT	6	0.15	No Hit
CAACAACTGTCCCAAGAACTATGTCTCTTGTATTGCTCGGTTCTTGGGTA	6	0.15	No Hit
GGTCCTATACTGGACAAATGCTGACGGACCACGATGGTGCCACGTATAAT	6	0.15	No Hit
AAGAGACTTCTTTTTTTTTGTATCATATCATACATATATAACAAACAGTC	6	0.15	No Hit
GAAATGGAGGAGGAGCAAGATAAATTGTTGCCTATTGCCAACGTGGGTCG	6	0.15	No Hit
GTGCAAGGAAGGTGCTTATTGGGGAACTGTTGCTGGCATGTATGTTGGGA	6	0.15	No Hit
GGCTGTTATCTTCCCGCGACTCATATCACTCTCTCTCTCTTTCTTCCTCT	6	0.15	No Hit
GAGAGATCCAAGAAATTTTCAACTCCAAGAATCTCTGTTCAGTTCAGATC	5	0.125	No Hit
TGGACTTGGCATAGAGGAAATTGTGAACCACATTTTACAAGGTTGGGAAG	5	0.125	No Hit
CCAATGGATAGGGAGGCAAGAGTCCTGAGATACAGAGAGAAAAAGAAGAC	5	0.125	No Hit
GCTGCTGTAAGCCATCAGATAGCTGTGGCTTTCTTTATAAATCACCGATT	5	0.125	No Hit
GTTTTCTGCGCTTAGCCTAAAGCAGTTCACTCTGAAAAGCGCCGTTGCTT	5	0.125	No Hit
CAAGTGCAAGCAGTCACAGCCTGACATTCAGAGGATCATAGAAAGCACCA	5	0.125	No Hit
CTGGTGTGTGTGCTTATCCTTTTGCCGTGCCGGAGTTCAGGCCAGGGTGG	5	0.125	No Hit
GTTGGCTTAATTTGTAGACATTTGGGATAGGATAACGTTTCTTGCTTCTT	5	0.125	No Hit
AGCAGGTTTCCGAATGGCAGCAGAATGTGCTCGTAATGCTTTGCTGCAAA	5	0.125	No Hit
AAATCTGACAACAGCAATGTTATTTGGAGCTCGCATGTGTGTCATGGGAT	5	0.125	No Hit
GCAACTCCTTTTAGGACAGATGCATCTGCGTTGGGCTCTTACACACCACC	5	0.125	No Hit
GAGTTGCGTGCCTTGACTTCAATCTTCAGAAGACAAAGATGCAATTGGGT	5	0.125	No Hit
GATAGGGGCAGGAGCTGCAGAAGGAGCTATTGATGCTGCTAATATCTTGA	5	0.125	No Hit
GAGCATACTTCAGTTGCTCTCAATGTTTCTTCAATAAGAATGATTCAGAT	5	0.125	No Hit
ATCAATCCGCCTTTCTCTTCCGGCTCCGCTCCCAAACCCCTAAAAATTAT	5	0.125	No Hit
GTCGATTTTAGACATTGAATTCATAGGCAGAATCCTGTCTGGAATGAAGA	5	0.125	No Hit
CCTATCCCCTACGAGGCTACCCGGATCGATGACGCGAATTGGGGACATTC	5	0.125	No Hit
CCATTTCACTGCGTGGGCCCTAGCGAAGTCTCCGCTTTTGATTGGCACCA	5	0.125	No Hit
ACTGAAGCCTCCTCTTGGGAAACATCGTCTAAAGATTGTGGAATTCATTG	5	0.125	No Hit
GATTCTAAGCACCGTAAAACACTTGCTGAGCTGGAACAGGGTGTAGATGG	5	0.125	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	0.0	0.0
16-17	0.0	0.0	0.0	0.0	0.0
18-19	0.0	0.0	0.0	0.0	0.0
20-21	0.0	0.0	0.0	0.0	0.0
22-23	0.0	0.0	0.0	0.0	0.0
24-25	0.0	0.0	0.0	0.0	0.0
26-27	0.0	0.0	0.0	0.0	0.0
28-29	0.0	0.0	0.0	0.0	0.0
30-31	0.0	0.0	0.0	0.0	0.0
32-33	0.0	0.0	0.0	0.0	0.0
34-35	0.0	0.0	0.0	0.0	0.0
36-37	0.0	0.0	0.0	0.0	0.0
38-39	0.0	0.0	0.0	0.0	0.0
40-41	0.0	0.0	0.0	0.0	0.0
42-43	0.0	0.0	0.0	0.0	0.0
44-45	0.0	0.0	0.0	0.0	0.0
46-47	0.0	0.0	0.0	0.0	0.0
48-49	0.0	0.0	0.0	0.0	0.0
50-51	0.0	0.0	0.0	0.0	0.0
52-53	0.0	0.0	0.0	0.0	0.0
54-55	0.0	0.0	0.0	0.0	0.0
56-57	0.0	0.0	0.0	0.0	0.0
58-59	0.0	0.0	0.0	0.0	0.0
60-61	0.037500000000000006	0.0	0.0	0.0	0.0
62-63	0.1	0.0	0.0	0.0	0.0
64-65	0.1	0.0	0.0	0.0	0.0
66-67	0.1	0.0	0.0	0.0	0.0
68-69	0.1	0.0	0.0	0.0	0.0
70-71	0.1125	0.0	0.0	0.0	0.0
72-73	0.125	0.0	0.0	0.0	0.0
74-75	0.1375	0.0	0.0	0.0	0.0
76-77	0.15	0.0	0.0	0.0	0.0
78-79	0.16249999999999998	0.0	0.0	0.0	0.0
80-81	0.175	0.0	0.0	0.0	0.0
82-83	0.2	0.0	0.0	0.0	0.0
84-85	0.2875	0.0	0.0	0.0	0.0
86-87	0.5	0.0	0.0	0.0	0.0
88-89	0.6625000000000001	0.0	0.0	0.0	0.0
90-91	0.8875	0.0	0.0	0.0	0.0
92-93	1.175	0.0	0.0	0.0	0.0
94-95	1.2875	0.0	0.0	0.0	0.0
96-97	1.475	0.0	0.0	0.0	0.0
98-99	1.5875	0.0	0.0	0.0	0.0
100-101	1.9	0.0	0.0	0.0	0.0
102-103	2.1625	0.0	0.0	0.0	0.0
104-105	2.475	0.0	0.0	0.0	0.0
106-107	2.975	0.0	0.0	0.0	0.0
108-109	3.4000000000000004	0.0	0.0	0.0	0.0
110-111	3.7375	0.0	0.0	0.0	0.0
112-113	4.125	0.0	0.0	0.0	0.0
114-115	4.9125	0.0	0.0	0.0	0.0
116-117	5.375	0.0	0.0	0.0	0.0
118-119	6.1	0.0	0.0	0.0	0.0
120-121	7.012499999999999	0.0	0.0	0.0	0.0
122-123	7.5375	0.0	0.0	0.0	0.0
124-125	8.025	0.0	0.0	0.0	0.0
126-127	8.5	0.0	0.0	0.0	0.0
128-129	9.2625	0.0	0.0	0.0	0.0
130-131	10.024999999999999	0.0	0.0	0.0	0.0
132-133	10.6375	0.0	0.0	0.0	0.0
134-135	11.3875	0.0	0.0	0.0	0.0
136-137	12.125	0.0	0.0	0.0	0.0
138-139	12.925	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGGTCGT	10	0.006830828	145.0	6
GTCGTTG	10	0.006830828	145.0	8
AGGGTCG	10	0.006830828	145.0	5
AAGGGTC	10	0.006830828	145.0	4
GTAAGGG	10	0.006830828	145.0	2
GGTCGTT	10	0.006830828	145.0	7
TAAGGGT	10	0.006830828	145.0	3
GGTAAGG	10	0.006830828	145.0	1
GGAGATC	40	0.0076550315	18.125	140-144
GAGATCG	80	0.0020131238	12.6875	140-144
>>END_MODULE
Read 2689513 spots for SRR26075374.sra
Written 2689513 spots for SRR26075374.sra
Read 2689513 spots for SRR26075374.sra
Written 2689513 spots for SRR26075374.sra
Read 2689513 spots for SRR26075374.sra
Written 2689513 spots for SRR26075374.sra
Read 2689513 spots for SRR26075374.sra
Written 2689513 spots for SRR26075374.sra
Read 2689513 spots for SRR26075374.sra
Written 2689513 spots for SRR26075374.sra
Read 2689513 spots for SRR26075374.sra
Written 2689513 spots for SRR26075374.sra
Read 2689513 spots for SRR26075374.sra
Written 2689513 spots for SRR26075374.sra
Read 2689513 spots for SRR26075374.sra
Written 2689513 spots for SRR26075374.sra
Read 2689513 spots for SRR26075374.sra
Written 2689513 spots for SRR26075374.sra
Read 2689513 spots for SRR26075374.sra
Written 2689513 spots for SRR26075374.sra
Read 2689513 spots for SRR26075374.sra
Written 2689513 spots for SRR26075374.sra
Read 2689513 spots for SRR26075374.sra
Written 2689513 spots for SRR26075374.sra
Read 2689513 spots for SRR26075374.sra
Written 2689513 spots for SRR26075374.sra
Read 2689513 spots for SRR26075374.sra
Written 2689513 spots for SRR26075374.sra
Read 2689513 spots for SRR26075374.sra
Written 2689513 spots for SRR26075374.sra
Read 2689513 spots for SRR26075374.sra
Written 2689513 spots for SRR26075374.sra
Read 2689513 spots for SRR26075374.sra
Written 2689513 spots for SRR26075374.sra
Read 2689513 spots for SRR26075374.sra
Written 2689513 spots for SRR26075374.sra
Read 2689513 spots for SRR26075374.sra
Written 2689513 spots for SRR26075374.sra
Read 2689513 spots for SRR26075374.sra
Written 2689513 spots for SRR26075374.sra
SRR ids: ['SRR26075374.sra']
extra args: ['--split-files', '--defline-qual', '+']
tempdir: /tmp/pfd_f18of_53
SRR26075374.sra spots: 53790260
blocks: [[1, 2689513], [2689514, 5379026], [5379027, 8068539], [8068540, 10758052], [10758053, 13447565], [13447566, 16137078], [16137079, 18826591], [18826592, 21516104], [21516105, 24205617], [24205618, 26895130], [26895131, 29584643], [29584644, 32274156], [32274157, 34963669], [34963670, 37653182], [37653183, 40342695], [40342696, 43032208], [43032209, 45721721], [45721722, 48411234], [48411235, 51100747], [51100748, 53790260]]
SRR26075374 file size 19869755
SRR26075374 completed basic pipeline successfully
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	skewer -f sanger -l 18 -q 10 -k inf -t 20 -o SRR26075374 SRR26075374_1.fastq SRR26075374_2.fastq
Input file:	SRR26075374_1.fastq
Paired file:	SRR26075374_2.fastq
trimmed:	SRR26075374-trimmed-pair1.fastq, SRR26075374-trimmed-pair2.fastq

Parameters used:
-- 3' end adapter sequence (-x):	AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC
-- paired 3' end adapter sequence (-y):	AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- end quality threshold (-q):		10
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ 
-- number of concurrent threads (-t):	20
Tue Feb 11 23:14:48 2025 >> started

Tue Feb 11 23:16:24 2025 >> done (95.765s)
53790260 read pairs processed; of these:
     276 ( 0.00%) short read pairs filtered out after trimming by size control
  161657 ( 0.30%) empty read pairs filtered out after trimming by size control
53628327 (99.70%) read pairs available; of these:
 9684882 (18.06%) trimmed read pairs available after processing
43943445 (81.94%) untrimmed read pairs available after processing

Length distribution of reads after trimming:
length	count	percentage
 18	      16	  0.00%
 19	      22	  0.00%
 20	      28	  0.00%
 21	      34	  0.00%
 22	      27	  0.00%
 23	      38	  0.00%
 24	      57	  0.00%
 25	      46	  0.00%
 26	      56	  0.00%
 27	      73	  0.00%
 28	      62	  0.00%
 29	      77	  0.00%
 30	      87	  0.00%
 31	      91	  0.00%
 32	      88	  0.00%
 33	      70	  0.00%
 34	      74	  0.00%
 35	     113	  0.00%
 36	      97	  0.00%
 37	      96	  0.00%
 38	     114	  0.00%
 39	     133	  0.00%
 40	     158	  0.00%
 41	     140	  0.00%
 42	     181	  0.00%
 43	     204	  0.00%
 44	     181	  0.00%
 45	     207	  0.00%
 46	     207	  0.00%
 47	     283	  0.00%
 48	     311	  0.00%
 49	     361	  0.00%
 50	     360	  0.00%
 51	     467	  0.00%
 52	     473	  0.00%
 53	     540	  0.00%
 54	     516	  0.00%
 55	     604	  0.00%
 56	     698	  0.00%
 57	     784	  0.00%
 58	     933	  0.00%
 59	    1107	  0.00%
 60	    1345	  0.00%
 61	    1452	  0.00%
 62	    1541	  0.00%
 63	    1906	  0.00%
 64	    2128	  0.00%
 65	    2309	  0.00%
 66	    2803	  0.01%
 67	    2994	  0.01%
 68	    3442	  0.01%
 69	    3787	  0.01%
 70	    4499	  0.01%
 71	    5173	  0.01%
 72	    6117	  0.01%
 73	    6877	  0.01%
 74	    8093	  0.02%
 75	    8961	  0.02%
 76	    9866	  0.02%
 77	   11033	  0.02%
 78	   11804	  0.02%
 79	   13851	  0.03%
 80	   15194	  0.03%
 81	   17271	  0.03%
 82	   19905	  0.04%
 83	   22029	  0.04%
 84	   24972	  0.05%
 85	   27756	  0.05%
 86	   29474	  0.05%
 87	   31863	  0.06%
 88	   34634	  0.06%
 89	   36108	  0.07%
 90	   39249	  0.07%
 91	   42833	  0.08%
 92	   46939	  0.09%
 93	   50514	  0.09%
 94	   55714	  0.10%
 95	   59563	  0.11%
 96	   62972	  0.12%
 97	   67195	  0.13%
 98	   68188	  0.13%
 99	   71648	  0.13%
100	   74580	  0.14%
101	   78084	  0.15%
102	   82837	  0.15%
103	   89373	  0.17%
104	   93877	  0.18%
105	   98092	  0.18%
106	  103219	  0.19%
107	  108582	  0.20%
108	  110797	  0.21%
109	  113802	  0.21%
110	  114048	  0.21%
111	  118857	  0.22%
112	  122288	  0.23%
113	  127141	  0.24%
114	  132910	  0.25%
115	  138493	  0.26%
116	  142419	  0.27%
117	  146710	  0.27%
118	  150327	  0.28%
119	  151895	  0.28%
120	  153750	  0.29%
121	  158970	  0.30%
122	  160966	  0.30%
123	  165623	  0.31%
124	  173473	  0.32%
125	  175687	  0.33%
126	  182319	  0.34%
127	  186397	  0.35%
128	  187539	  0.35%
129	  190294	  0.35%
130	  192772	  0.36%
131	  194168	  0.36%
132	  196321	  0.37%
133	  203658	  0.38%
134	  204673	  0.38%
135	  207998	  0.39%
136	  212889	  0.40%
137	  217955	  0.41%
138	  221387	  0.41%
139	  224442	  0.42%
140	  225630	  0.42%
141	  228056	  0.43%
142	  230502	  0.43%
143	  231413	  0.43%
144	  235734	  0.44%
145	  239982	  0.45%
146	  239521	  0.45%
147	  246375	  0.46%
148	  250375	  0.47%
149	  249417	  0.47%
150	  254049	  0.47%
151	43943445	 81.94%
53628327 reads passed initial QC


criterion=sequence-density
sequence-density=0.31
sequence-density-rank=1
fanout-score=2.77
fanout-score-rank=32
prefix-density=0.40
prefix-fanout=2.1
sequence=CACTTGCAGCCATTCTC


criterion=fanout-score
sequence-density=0.03
sequence-density-rank=38
fanout-score=344.50
fanout-score-rank=1
prefix-density=0.59
prefix-fanout=19.7
sequence=CCACCACCACCTCCACCACTTCCGCGGGATTGAGCTTCGTTCACGGTGATGTTACGGCCATCGAGGTCCTGACCGTTCATTCCATCAATAGCATCTCTCATTGCCTTCTC


criterion=sequence-density
sequence-density=0.33
sequence-density-rank=1
fanout-score=2.45
fanout-score-rank=39
prefix-density=0.34
prefix-fanout=2.4
sequence=ATGTACCCTGAC


criterion=fanout-score
sequence-density=0.03
sequence-density-rank=36
fanout-score=346.24
fanout-score-rank=1
prefix-density=0.60
prefix-fanout=17.8
sequence=AAGAAAGAAATACACAATGGCAGGAATCATGCACAAGATTGAGGAGACTCTGAACATTGGAGGCAAGAAAGATGAGCGCAAGGGTGAGACACAAGGTGGGTACAACCAACA
SRR26075374 testing PE reads STAR mapping to Ensembl genome
                                 Started job on |	Feb 11 23:17:09
                             Started mapping on |	Feb 11 23:17:09
                                    Finished on |	Feb 11 23:34:57
       Mapping speed, Million of reads per hour |	180.77

                          Number of input reads |	53628327
                      Average input read length |	292
                                    UNIQUE READS:
                   Uniquely mapped reads number |	46789320
                        Uniquely mapped reads % |	87.25%
                          Average mapped length |	291.32
                       Number of splices: Total |	43374186
            Number of splices: Annotated (sjdb) |	42231328
                       Number of splices: GT/AG |	42547861
                       Number of splices: GC/AG |	641097
                       Number of splices: AT/AC |	41198
               Number of splices: Non-canonical |	144030
                      Mismatch rate per base, % |	0.40%
                         Deletion rate per base |	0.03%
                        Deletion average length |	3.15
                        Insertion rate per base |	0.02%
                       Insertion average length |	2.52
                             MULTI-MAPPING READS:
        Number of reads mapped to multiple loci |	1264047
             % of reads mapped to multiple loci |	2.36%
        Number of reads mapped to too many loci |	159475
             % of reads mapped to too many loci |	0.30%
                                  UNMAPPED READS:
       % of reads unmapped: too many mismatches |	0.00%
                 % of reads unmapped: too short |	9.77%
                     % of reads unmapped: other |	0.33%
                                  CHIMERIC READS:
                       Number of chimeric reads |	0
                            % of chimeric reads |	0.00%
N_unmapped	5574960	5574960	5574960
N_multimapping	1264047	1264047	1264047
N_noFeature	1273632	46275460	1565779
N_ambiguous	487921	3069	264052
UnstrandedReadsAssigned:45027767 PositiveStrandReadsAssigned:510791 NegativeStrandReadsAssigned:44959489
Dataset is classified negative stranded
MeadianReadLen=151 20thPercentileLength=151 echo kmer=147
SRR26075374 Starting Kallisto paired end mapping to ensembl reference transcriptome

[quant] fragment length distribution will be estimated from the data
[index] k-mer length: 31
[index] number of targets: 52,400
[index] number of k-mers: 62,057,036
[index] number of equivalence classes: 130,681
[quant] running in paired-end mode
[quant] will process pair 1: SRR26075374-trimmed-pair1.fastq
                             SRR26075374-trimmed-pair2.fastq
[quant] finding pseudoalignments for the reads ... done
[quant] processed 53,628,327 reads, 45,586,101 reads pseudoaligned
[quant] estimated average fragment length: 207.098
[   em] quantifying the abundances ... done
[   em] the Expectation-Maximization algorithm ran for 1,197 rounds

  52401 SRR26075374.ke.tsv
  34699 SRR26075374.se.tsv
  87100 total
==> SRR26075374.ke.tsv <==
target_id	length	eff_length	est_counts	tpm
Potri.005G200100.1.v4.1	2018	1811.9	5537	62.3473
Potri.005G024800.1.v4.1	1035	828.902	3172	78.0743
Potri.004G059700.1.v4.1	961	754.902	0	0
Potri.007G009000.2.v4.1	1416	1209.9	0	0
Potri.003G141000.2.v4.1	2943	2736.9	1896.37	14.1365
Potri.016G087400.1.v4.1	270	92.9782	3066.56	672.897
Potri.015G069301.1.v4.1	564	359.224	0	0
Potri.010G195200.1.v4.1	1773	1566.9	1773	23.0858
Potri.012G127500.1.v4.1	977	770.902	62118	1643.98

==> SRR26075374.se.tsv <==
Potri.001G166300.v4.1	0
Potri.001G448400.v4.1	85
Potri.001G233950.v4.1	0
Potri.001G122700.v4.1	604
Potri.001G212900.v4.1	0
Potri.001G182400.v4.1	0
Potri.001G256600.v4.1	0
Potri.001G040500.v4.1	5
Potri.001G416900.v4.1	0
Potri.001G452600.v4.1	3005
SRR26075374 completed mapping pipeline successfully