Starting /dee2/code/volunteer_pipeline.sh SRR26075375 current disk space = 2810602356736 free memory = 1576994264 SRR26075375 SRAfilesize bd62b024d7582984651cabb44f62d69a SRR26075375.sra SRR26075375.sra file validated SRR26075375 is paired end SRR26075375 is conventional basespace SRR26075375 read1 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR26075375_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.562 37.0 37.0 37.0 37.0 37.0 2 36.541 37.0 37.0 37.0 37.0 37.0 3 36.6045 37.0 37.0 37.0 37.0 37.0 4 36.698 37.0 37.0 37.0 37.0 37.0 5 36.704 37.0 37.0 37.0 37.0 37.0 6 36.714 37.0 37.0 37.0 37.0 37.0 7 36.6185 37.0 37.0 37.0 37.0 37.0 8 36.585 37.0 37.0 37.0 37.0 37.0 9 36.6505 37.0 37.0 37.0 37.0 37.0 10-14 36.60755 37.0 37.0 37.0 37.0 37.0 15-19 36.5939 37.0 37.0 37.0 37.0 37.0 20-24 36.523300000000006 37.0 37.0 37.0 37.0 37.0 25-29 36.4923 37.0 37.0 37.0 37.0 37.0 30-34 36.4493 37.0 37.0 37.0 37.0 37.0 35-39 36.405699999999996 37.0 37.0 37.0 37.0 37.0 40-44 36.3341 37.0 37.0 37.0 37.0 37.0 45-49 36.3215 37.0 37.0 37.0 37.0 37.0 50-54 36.258399999999995 37.0 37.0 37.0 37.0 37.0 55-59 36.1854 37.0 37.0 37.0 37.0 37.0 60-64 36.1754 37.0 37.0 37.0 37.0 37.0 65-69 36.091899999999995 37.0 37.0 37.0 37.0 37.0 70-74 36.1408 37.0 37.0 37.0 37.0 37.0 75-79 36.1255 37.0 37.0 37.0 37.0 37.0 80-84 36.0536 37.0 37.0 37.0 37.0 37.0 85-89 35.9697 37.0 37.0 37.0 37.0 37.0 90-94 35.8757 37.0 37.0 37.0 37.0 37.0 95-99 35.862700000000004 37.0 37.0 37.0 37.0 37.0 100-104 35.8574 37.0 37.0 37.0 37.0 37.0 105-109 35.8086 37.0 37.0 37.0 37.0 37.0 110-114 35.6649 37.0 37.0 37.0 37.0 37.0 115-119 35.6481 37.0 37.0 37.0 37.0 37.0 120-124 35.633500000000005 37.0 37.0 37.0 37.0 37.0 125-129 35.48960000000001 37.0 37.0 37.0 37.0 37.0 130-134 35.384699999999995 37.0 37.0 37.0 34.6 37.0 135-139 35.2456 37.0 37.0 37.0 29.8 37.0 140-144 35.0391 37.0 37.0 37.0 27.4 37.0 145-149 35.018 37.0 37.0 37.0 25.0 37.0 150-151 34.81175 37.0 37.0 37.0 25.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.0 1101 2 0.0 1101 3 0.0 1101 4 0.0 1101 5 0.0 1101 6 0.0 1101 7 0.0 1101 8 0.0 1101 9 0.0 1101 10-14 0.0 1101 15-19 0.0 1101 20-24 0.0 1101 25-29 0.0 1101 30-34 0.0 1101 35-39 0.0 1101 40-44 0.0 1101 45-49 0.0 1101 50-54 0.0 1101 55-59 0.0 1101 60-64 0.0 1101 65-69 0.0 1101 70-74 0.0 1101 75-79 0.0 1101 80-84 0.0 1101 85-89 0.0 1101 90-94 0.0 1101 95-99 0.0 1101 100-104 0.0 1101 105-109 0.0 1101 110-114 0.0 1101 115-119 0.0 1101 120-124 0.0 1101 125-129 0.0 1101 130-134 0.0 1101 135-139 0.0 1101 140-144 0.0 1101 145-149 0.0 1101 150-151 0.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 17 1.0 18 0.0 19 1.0 20 1.0 21 4.0 22 3.0 23 2.0 24 6.0 25 6.0 26 11.0 27 5.0 28 16.0 29 15.0 30 31.0 31 47.0 32 55.0 33 114.0 34 170.0 35 466.0 36 2848.0 37 198.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.84792396198099 12.631315657828916 7.20360180090045 34.31715857928965 2 21.099999999999998 14.000000000000002 34.525 30.375000000000004 3 19.8 19.775000000000002 29.599999999999998 30.825000000000003 4 21.65 27.075 25.8 25.474999999999998 5 23.175 32.425 24.925 19.475 6 20.200000000000003 35.8 22.875 21.125 7 14.224999999999998 28.299999999999997 42.6 14.875 8 18.275 26.125 31.25 24.349999999999998 9 16.275000000000002 25.224999999999998 33.900000000000006 24.6 10-14 19.545977298864944 29.181459072953647 28.21641082054103 23.05615280764038 15-19 20.505000000000003 28.54 27.305 23.65 20-24 19.794999999999998 28.03 27.905 24.27 25-29 19.814999999999998 29.15 27.57 23.465 30-34 19.7 29.404999999999998 26.665 24.23 35-39 19.54 28.925 27.755000000000003 23.78 40-44 19.855 28.835 27.639999999999997 23.669999999999998 45-49 20.064999999999998 28.815 27.310000000000002 23.810000000000002 50-54 20.215 28.575 27.485 23.724999999999998 55-59 21.4 28.000000000000004 27.41 23.189999999999998 60-64 20.03 28.765 27.1 24.104999999999997 65-69 20.815 27.35 28.050000000000004 23.785 70-74 19.875 28.994999999999997 27.875 23.255 75-79 20.25 28.199999999999996 27.750000000000004 23.799999999999997 80-84 19.97 28.310000000000002 28.24 23.48 85-89 20.24 29.07 27.36 23.330000000000002 90-94 20.925 29.29 26.63 23.155 95-99 20.62 27.750000000000004 26.895000000000003 24.735 100-104 20.945 27.47 27.99 23.595 105-109 20.72 28.205000000000002 28.075 23.0 110-114 20.18 28.37 27.485 23.965 115-119 20.474999999999998 28.98 27.445000000000004 23.1 120-124 20.635 27.705000000000002 27.6 24.060000000000002 125-129 21.46 28.560000000000002 25.81 24.169999999999998 130-134 21.81 27.800000000000004 26.484999999999996 23.905 135-139 20.745 27.935 26.529999999999998 24.79 140-144 21.955 27.384999999999998 27.055 23.605 145-149 21.68 27.63 26.240000000000002 24.45 150-151 20.7 27.212500000000002 26.137500000000003 25.95 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 1.0 7 1.0 8 0.0 9 0.5 10 1.0 11 1.5 12 1.0 13 1.0 14 1.0 15 0.5 16 0.5 17 0.0 18 1.0 19 3.5 20 3.0 21 1.0 22 1.5 23 1.5 24 2.0 25 1.5 26 8.5 27 10.5 28 5.0 29 9.5 30 21.5 31 30.5 32 40.0 33 50.0 34 61.5 35 75.5 36 108.0 37 126.5 38 138.0 39 154.0 40 155.0 41 164.5 42 211.0 43 251.0 44 242.5 45 243.5 46 249.5 47 242.5 48 247.5 49 225.0 50 173.5 51 157.5 52 137.0 53 101.5 54 88.5 55 69.5 56 39.0 57 27.5 58 24.0 59 23.5 60 19.0 61 13.0 62 9.0 63 3.0 64 2.0 65 2.0 66 3.5 67 3.0 68 1.0 69 2.5 70 2.0 71 1.5 72 1.0 73 0.5 74 0.5 75 0.0 76 0.0 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.05 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.005 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150-151 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 59.175 #Duplication Level Percentage of deduplicated Percentage of total 1 61.34347275031685 36.3 2 22.729193071398395 26.900000000000002 3 8.660752006759612 15.375 4 3.5487959442332064 8.4 5 2.2391212505280946 6.625 6 0.5069708491761723 1.7999999999999998 7 0.5914659907055344 2.45 8 0.08449514152936206 0.4 9 0.16899028305872413 0.8999999999999999 >10 0.12674271229404308 0.8500000000000001 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GGGGTCTCTTTCCAATCGATGCGCGTGTTGACAAAGGCTGAATTGTCATG 12 0.3 No Hit TGATATTGATGTGATTGAAGATGATTTCTTTTCTAATAAATCTTCCAAAT 12 0.3 No Hit GGGAAGATTTTATGCTTTGAGCAATTAAAAGACCTGCTGATTTGCATTGG 10 0.25 No Hit GATGCAATGGCTTTACAGCTTGGCGTTCAATATGCACAATCTGCCCAACA 9 0.22499999999999998 No Hit GTGCGGAGGCAATGGCGCTAGTACCAAAGGAAGAATCAACAAAGATGCAG 9 0.22499999999999998 No Hit CCTCACTCTAACCATGTCCTGCTTATCATGCCTAGTTACATCATTGTTTA 9 0.22499999999999998 No Hit CATAGCTGCCATGTGCTCCCCAGATTGCACATGTTGAAACCCAACAAACC 9 0.22499999999999998 No Hit GCAACATTATACACATGCACATTCGCAAGCCAAACCAAGAACAGAAATTT 8 0.2 No Hit CGCTTTTCTAAATTACAAAAGAATGCATTGAGCACAATTCTAGTCATCTG 8 0.2 No Hit ATCCTTTGAAAACACAACCAAAGCCACCTTCACCAAGAAGACTATCGGGA 7 0.17500000000000002 No Hit GCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGA 7 0.17500000000000002 No Hit CCGCAAGTAATAAGGACTACAAACAAGTCAGCAAGATATAAGAAAGATCA 7 0.17500000000000002 No Hit AAAATTTCCTTGGCTGCCTTAGCATTGAGTTGTTCAGCAGCCGACCATTT 7 0.17500000000000002 No Hit GTGGGATTCCCTCCTTGTCCTGGATCTTGGCCTTAACATTATCAATAGTG 7 0.17500000000000002 No Hit CGACCTTTTGATACCGTTTATGAGAATAGAAGCCCCATTTGCACCAGATA 7 0.17500000000000002 No Hit GTTGCAGTTAAAGCAGAGAACATCTGTTTGATTATCCCATGCATTACAGT 7 0.17500000000000002 No Hit ATTCAAAATACAGTAGCACATGGCATGGTATGACAGTCTTTCAAATTTAT 7 0.17500000000000002 No Hit GGCTTCTTGCAACTTGCAGCCACACCTCGTCCAACTCAACAGCATCTCAA 7 0.17500000000000002 No Hit GTCATCATTGACCTCTGACATTGACTTCTCAGACTCCTCTTCTTCCTTGG 7 0.17500000000000002 No Hit CCTTGCTTAAATTGAAAAAGAAACCATGTCTTAATAACAAAAGTAGATAT 7 0.17500000000000002 No Hit CGTGACTGTAACTCCTGCATCTTTCAATATTAATCCCATCTTTGTGCAAT 7 0.17500000000000002 No Hit CGGGGGCGGTCCTTGTGGGAATCGCTTGGTGTCAGTGCAATAGTTATAAA 7 0.17500000000000002 No Hit GGCTACTGGATCTGGGGGTGTGTCATTTGCACCAGCCCTATTTGTACTAG 7 0.17500000000000002 No Hit CCTCACCAAACCCCTACGAAGATCTCCAAAAACCTATATCTAATCTACCC 6 0.15 No Hit CGCGTGTTGACAAAGGCTGAATTGTCATGAGAGACAAGTGAGGATGAAGG 6 0.15 No Hit AAGATGAATAAAAATGGAGAAATTTTGCAGGGATAATAAAAAGGCGTTTG 6 0.15 No Hit GTTCGATGTCTTCGAATAGATCAGTAACCTCAGTTTCTAGGTCGAGATTA 6 0.15 No Hit GCAGTTTCCAGAAACGTGTATCACATCTAGGCATGGAATCTTATGCCAGC 6 0.15 No Hit AGTGACGATCCGTGCACAACCTTGTTCTCAAATAGGATGTGTAATGCCAC 6 0.15 No Hit GCACGTTTAGCAAGCAAATTCCATCGGCCTTCTCCATGACGAGCAATGTA 6 0.15 No Hit CTCGAACACACTACTTTTAGTTGGTAATTCAAACTTCTTCAAAAACCAAG 6 0.15 No Hit GCCCACTGGTATTAACCGAGAACAATAGAAACACAGGACAGCCACCAGAT 6 0.15 No Hit CTCCACTTTCACTTCCTCTTTTTTCAGCCCTGGAAGATCAGCCTTGAAAA 6 0.15 No Hit CCATCTCGCGGGAGCTTATCTCATTGAATATCTGACAAAAGACGAATGAA 6 0.15 No Hit AGGCAAAGTAATATATAATAACGTAGGAGACATATAAAGAAAGCGGGAGA 6 0.15 No Hit GTGGTGGTGTTGTTGTTGTTGATGATCTTGGGAGGACTTTTCCTCTTCAA 5 0.125 No Hit CCCTCATGCGGAAGCCATAAAACCACCTGCCAAGCTTCCACCATTTAACA 5 0.125 No Hit GCGTCGAGCTGCCTCGTTGCTGTCGATAACTGCTGTTGCAGGAATTTGGT 5 0.125 No Hit AACCTGGTTATTATTATTAATTTATTTTGAACTCTGATTTGATTTTCCGA 5 0.125 No Hit CAACAATTGTACCTAGACTGCCAGTAAACACAAGATGCATACTTCAACTA 5 0.125 No Hit GTGATTTCAAGTTCGCATGTTTACCACAATATACCTCTGCATGATTAGCA 5 0.125 No Hit GTAACACTTCCATACAAATTGTGAGAATCTCACAGCCCAGCTATTGGCTA 5 0.125 No Hit CAACCTCTACTGTGGCCTAACCCTATGGCTCAACACCATTTCCCTAAGTT 5 0.125 No Hit CATTCTTCGGCCCTGTTATCCAGTTTGAGGCCCCAGTCAAATCCCTTTCT 5 0.125 No Hit CATGTTAGCTTGGAGTGGCACCAACTCCGAGGAGAAACTCTGCTCTGGTG 5 0.125 No Hit GTTCATTGGCATGTGGTGAAATGCGACTCCTGACCAAGACTTCCGAGCAT 5 0.125 No Hit GGTGGAGTCCCAGGAGCGTGATGATGTCTCATTGGGGACTGTCGGACAGG 5 0.125 No Hit CTTTGGGAAGTACAATTCTGCTGCAATTTCCAGGATGATTACGCGACCTT 5 0.125 No Hit CTAATAAACCAGCAAATTGGAATGGTTGTAAGATATCAACACCACCTGTT 5 0.125 No Hit TCCTTAAGATGGCAACCCGCCACTAGTCCAACTGCCACAAGAGGGCATAC 5 0.125 No Hit CTTTACTTCAATTAGTATTCTGTCCATTCTCGACACCAAGACCGTACAAA 5 0.125 No Hit GTCGAGCATCATGATGGATCTGCATACTTTTAAGATCAGGAGCTGTCGTC 5 0.125 No Hit CCGGTCTCTTGGAGAAATGACCATGTACACCCTGGGCAATATCAGGACTT 5 0.125 No Hit GAGAGAACGAGGAAAGCAGAAAAGAAAAAAAGACTAAAGACAACAGACAG 5 0.125 No Hit CTGTCCACTTGTCCTGTTGCCAATACTCGACACTCTTGTCAAAATCAACT 5 0.125 No Hit GTCTTTGTTCTGCCAGCTTCTTCCTACTCTGGTATCGAACCTTCTTCTCA 5 0.125 No Hit GCTTTCAGGACATTGTATATCAGCATTTTTCTTATTCGATTTATGACTGA 5 0.125 No Hit GGGAGTCTGGCCTTGAGCGGTTCCATCAGTTCCAGCGGTTCCAGCGGTTC 5 0.125 No Hit ACCGCAACGACTACAGCAACAGCAACAAGGAAACTACCATAAATCTACAC 5 0.125 No Hit GCGGACTTATCACTCGCATACTGAACATCACAAAATCCAATATGGTTAAG 5 0.125 No Hit CCTTGTTCTCCCCACCCAAAGTCACAATCAAATGAGTTGCTCCCCTTGTC 5 0.125 No Hit AGGACCCATTGCCGCAAAAAAGACTATCAACTAACAATATGACCATGGAA 5 0.125 No Hit GGAAGAAAAGAAAAAACTGAGTCAGATTAAGGTTGTAGGGTTTACAAGTG 5 0.125 No Hit GCAGGGTTTGGAGTGGAAACCGGAGAAAGAGCCAGATCTGGAGCTGGTGC 5 0.125 No Hit GAAAACAAAAGATGCCTCTTTAATGTCTCCATTCATTTTGTCGGCTATTC 5 0.125 No Hit GTCTCCGGTCACCAAAGGCCTCCGACCTTCTCTTTCGCGAATCACCCTCG 5 0.125 No Hit GCCAGACTTGAGGTGAGGCCAGAAGTAGCACCACTGCTTCCATAATTCTT 5 0.125 No Hit CTCACAATTACAAGCAATCTATTTAATTGAAATAACAATCCTGTTGCCTA 5 0.125 No Hit GGGGTGGATAACCTTGAGGAGGATAGCCTTGAGGGGGATATGCTCCAGGC 5 0.125 No Hit CATCAGCATTAGTCTGACACCGCAATGGGTGCCACTTGCATAACCTTCAC 5 0.125 No Hit CCTTCATGTCCATGGATATTGAATCCACCCCTGGAAGACCAGAGACTATA 5 0.125 No Hit CGGAGGAGTAGAACCAGCTGGTGACGCCGGGGGAGTAGCAGAGGAACCGT 5 0.125 No Hit GTGGACTAGTGTGTTGTAGATGGAGCAGCCAAGCCGAGCCACGAGAACTC 5 0.125 No Hit GTCCAGACAGAAGGTGCTCCGCTCCAAGGTTGGAAGTCATGATAATCACT 5 0.125 No Hit CCGGAAACTCCATCGATGACCATCGGTGTCGACGGCAACAACTTCTTTCG 5 0.125 No Hit CTGGATCATAGAGGGACCAAGGTGTCTTATAGAGACCTTCATTCCAGAAC 5 0.125 No Hit CAGGCAAGATTTGAGTAAAGCAGAGTAACAGAAGAGTTTAGACTAAAACG 5 0.125 No Hit GCTGGAAACCTGCCATCATGAATCTAGACTTCCACTTGCGAAAGAGCTCG 5 0.125 No Hit ATATGAGATCTCATCATCTTAAGTCGACACTAGAGACGATTTGGTCAGAG 5 0.125 No Hit GGTCATTTGGGACATTTGCACTGAATGTGGTGCAGCAACTGTGTTGGATG 5 0.125 No Hit CCGACTCCTACTAACCCTATATCCACGATATCTTCCAAACAACTTTCTCC 5 0.125 No Hit GGCATGGGGCACATCTGAAGCATTAGCTTGAGATTGTAATTGCAGTCTTT 5 0.125 No Hit CTCTTCACAGTACGTAATTGCCATCATGTCCATTGAGGTAGCCCCCCTCG 5 0.125 No Hit TTTTTTTTTTTTCCAAAGCAATGAGGCGAGTCCTATTTCCTGTACTATAA 5 0.125 No Hit GCCCCTATGCATACTGGATCAATGTCCCCTATCACTGTCATCTTCTTGTC 5 0.125 No Hit CTGGATCTCTTTTTTGCTAAGCTCTCACTACGGCGCTCACGCTGCTCCTT 5 0.125 No Hit GGAAAAATGAAACCAAGGCAAACTGCAGCAGTTGCTCCAGTGAATTGGAA 5 0.125 No Hit ACGGTAACAGTTTCGATATTACTTAATTTCTTGACACATGCAGGAAGAGC 5 0.125 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.025 0.0 0.0 0.0 0.0 60-61 0.025 0.0 0.0 0.0 0.0 62-63 0.025 0.0 0.0 0.0 0.0 64-65 0.025 0.0 0.0 0.0 0.0 66-67 0.025 0.0 0.0 0.0 0.0 68-69 0.037500000000000006 0.0 0.0 0.0 0.0 70-71 0.05 0.0 0.0 0.0 0.0 72-73 0.05 0.0 0.0 0.0 0.0 74-75 0.05 0.0 0.0 0.0 0.0 76-77 0.0875 0.0 0.0 0.0 0.0 78-79 0.1 0.0 0.0 0.0 0.0 80-81 0.1 0.0 0.0 0.0 0.0 82-83 0.15 0.0 0.0 0.0 0.0 84-85 0.2625 0.0 0.0 0.0 0.0 86-87 0.30000000000000004 0.0 0.0 0.0 0.0 88-89 0.4125 0.0 0.0 0.0 0.0 90-91 0.575 0.0 0.0 0.0 0.0 92-93 0.625 0.0 0.0 0.0 0.0 94-95 0.6875 0.0 0.0 0.0 0.0 96-97 0.825 0.0 0.0 0.0 0.0 98-99 1.0125000000000002 0.0 0.0 0.0 0.0 100-101 1.2875 0.0 0.0 0.0 0.0 102-103 1.475 0.0 0.0 0.0 0.0 104-105 1.85 0.0 0.0 0.0 0.0 106-107 2.2375 0.0 0.0 0.0 0.0 108-109 2.55 0.0 0.0 0.0 0.0 110-111 2.975 0.0 0.0 0.0 0.0 112-113 3.4875 0.0 0.0 0.0 0.0 114-115 4.15 0.0 0.0 0.0 0.0 116-117 4.525 0.0 0.0 0.0 0.0 118-119 4.8625 0.0 0.0 0.0 0.0 120-121 5.4625 0.0 0.0 0.0 0.0 122-123 6.25 0.0 0.0 0.0 0.0 124-125 6.762499999999999 0.0 0.0 0.0 0.0 126-127 7.5 0.0 0.0 0.0 0.0 128-129 8.25 0.0 0.0 0.0 0.0 130-131 8.8 0.0 0.0 0.0 0.0 132-133 9.5375 0.0 0.0 0.0 0.0 134-135 10.325 0.0 0.0 0.0 0.0 136-137 11.2625 0.0 0.0 0.0 0.0 138-139 12.6875 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content pass >>END_MODULE SRR26075375 read2 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR26075375_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.3685 37.0 37.0 37.0 37.0 37.0 2 36.2135 37.0 37.0 37.0 37.0 37.0 3 36.2175 37.0 37.0 37.0 37.0 37.0 4 36.324 37.0 37.0 37.0 37.0 37.0 5 36.278 37.0 37.0 37.0 37.0 37.0 6 36.136 37.0 37.0 37.0 37.0 37.0 7 36.2535 37.0 37.0 37.0 37.0 37.0 8 36.3335 37.0 37.0 37.0 37.0 37.0 9 36.328 37.0 37.0 37.0 37.0 37.0 10-14 36.24980000000001 37.0 37.0 37.0 37.0 37.0 15-19 36.1771 37.0 37.0 37.0 37.0 37.0 20-24 36.1965 37.0 37.0 37.0 37.0 37.0 25-29 36.0997 37.0 37.0 37.0 37.0 37.0 30-34 36.005399999999995 37.0 37.0 37.0 37.0 37.0 35-39 35.967699999999994 37.0 37.0 37.0 37.0 37.0 40-44 35.8931 37.0 37.0 37.0 37.0 37.0 45-49 35.8186 37.0 37.0 37.0 37.0 37.0 50-54 35.6287 37.0 37.0 37.0 37.0 37.0 55-59 35.659299999999995 37.0 37.0 37.0 37.0 37.0 60-64 35.7461 37.0 37.0 37.0 37.0 37.0 65-69 35.6783 37.0 37.0 37.0 37.0 37.0 70-74 35.601800000000004 37.0 37.0 37.0 37.0 37.0 75-79 35.5287 37.0 37.0 37.0 37.0 37.0 80-84 35.5082 37.0 37.0 37.0 37.0 37.0 85-89 35.522200000000005 37.0 37.0 37.0 37.0 37.0 90-94 35.440200000000004 37.0 37.0 37.0 37.0 37.0 95-99 35.4681 37.0 37.0 37.0 37.0 37.0 100-104 35.44109999999999 37.0 37.0 37.0 37.0 37.0 105-109 35.32470000000001 37.0 37.0 37.0 37.0 37.0 110-114 35.3082 37.0 37.0 37.0 34.6 37.0 115-119 35.29200000000001 37.0 37.0 37.0 32.2 37.0 120-124 35.080600000000004 37.0 37.0 37.0 25.0 37.0 125-129 35.1312 37.0 37.0 37.0 32.2 37.0 130-134 35.118100000000005 37.0 37.0 37.0 29.8 37.0 135-139 35.0186 37.0 37.0 37.0 25.0 37.0 140-144 35.01305 37.0 37.0 37.0 25.0 37.0 145-149 34.93955 37.0 37.0 37.0 25.0 37.0 150-151 34.629625000000004 37.0 37.0 37.0 25.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.0 1101 2 0.0 1101 3 0.0 1101 4 0.0 1101 5 0.0 1101 6 0.0 1101 7 0.0 1101 8 0.0 1101 9 0.0 1101 10-14 0.0 1101 15-19 0.0 1101 20-24 0.0 1101 25-29 0.0 1101 30-34 0.0 1101 35-39 0.0 1101 40-44 0.0 1101 45-49 0.0 1101 50-54 0.0 1101 55-59 0.0 1101 60-64 0.0 1101 65-69 0.0 1101 70-74 0.0 1101 75-79 0.0 1101 80-84 0.0 1101 85-89 0.0 1101 90-94 0.0 1101 95-99 0.0 1101 100-104 0.0 1101 105-109 0.0 1101 110-114 0.0 1101 115-119 0.0 1101 120-124 0.0 1101 125-129 0.0 1101 130-134 0.0 1101 135-139 0.0 1101 140-144 0.0 1101 145-149 0.0 1101 150-151 0.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 12 4.0 13 5.0 14 8.0 15 8.0 16 5.0 17 12.0 18 4.0 19 4.0 20 5.0 21 7.0 22 6.0 23 12.0 24 12.0 25 18.0 26 12.0 27 13.0 28 18.0 29 20.0 30 15.0 31 30.0 32 36.0 33 79.0 34 154.0 35 697.0 36 2593.0 37 223.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 47.8 22.900000000000002 9.1 20.200000000000003 2 29.9 22.725 28.425 18.95 3 23.0 25.5 34.050000000000004 17.45 4 26.674999999999997 31.85 21.375 20.1 5 25.275 33.45 22.475 18.8 6 23.625 40.150000000000006 21.45 14.774999999999999 7 22.625 22.8 35.9 18.675 8 22.2 25.374999999999996 26.650000000000002 25.775 9 23.7 25.900000000000002 28.325 22.075 10-14 24.235 29.17 25.335 21.26 15-19 24.104999999999997 28.144999999999996 26.400000000000002 21.349999999999998 20-24 25.135 27.435 26.985 20.445 25-29 24.535 28.675 26.695 20.095 30-34 23.855 27.355 27.665 21.125 35-39 25.765 27.955000000000002 26.5 19.78 40-44 24.044999999999998 27.994999999999997 28.23 19.73 45-49 24.465 28.294999999999998 27.345000000000002 19.895 50-54 23.990000000000002 27.994999999999997 28.265 19.75 55-59 23.68 28.144999999999996 27.189999999999998 20.985 60-64 25.035 28.465 26.61 19.89 65-69 24.325 27.675 27.515 20.485 70-74 23.195 29.085 27.189999999999998 20.53 75-79 23.200000000000003 29.185 27.665 19.950000000000003 80-84 24.44 28.875 27.034999999999997 19.650000000000002 85-89 23.84 28.560000000000002 27.365000000000002 20.235 90-94 24.38 28.89 27.075 19.655 95-99 23.445 28.63 28.025 19.900000000000002 100-104 24.005000000000003 28.555000000000003 27.275 20.165 105-109 24.16 28.875 27.07 19.895 110-114 24.89 29.395 26.825 18.89 115-119 25.27 28.525 26.465 19.74 120-124 25.21 29.595 25.825 19.37 125-129 25.264999999999997 29.07 26.145000000000003 19.52 130-134 26.165 28.12 26.340000000000003 19.375 135-139 25.740000000000002 28.055000000000003 26.955000000000002 19.25 140-144 26.87634381719086 28.05140257012851 26.346317315865793 18.72593629681484 145-149 25.888883332499873 28.564284642696403 26.153923088463273 19.39290893634045 150-151 26.703337917239654 27.965995749468686 25.203150393799223 20.127515939492437 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.5 5 1.0 6 1.0 7 1.5 8 1.5 9 1.5 10 2.0 11 2.0 12 1.5 13 1.0 14 1.5 15 1.5 16 1.0 17 0.5 18 0.5 19 2.0 20 1.5 21 1.0 22 2.0 23 1.5 24 0.5 25 3.5 26 6.5 27 6.5 28 8.0 29 7.5 30 8.5 31 18.0 32 26.0 33 40.5 34 72.0 35 76.0 36 92.5 37 132.0 38 136.0 39 156.0 40 189.0 41 202.0 42 222.0 43 236.5 44 246.5 45 254.0 46 238.0 47 248.5 48 245.0 49 201.0 50 172.0 51 133.0 52 106.5 53 95.5 54 78.0 55 71.5 56 54.5 57 33.0 58 24.5 59 19.0 60 17.0 61 10.0 62 5.5 63 7.0 64 5.5 65 2.5 66 0.5 67 3.0 68 3.5 69 4.5 70 3.5 71 1.0 72 2.5 73 1.5 74 0.5 75 2.0 76 2.5 77 1.5 78 0.5 79 1.0 80 1.0 81 0.0 82 0.5 83 1.5 84 2.5 85 3.0 86 3.0 87 2.0 88 0.5 89 0.0 90 1.0 91 2.0 92 1.5 93 1.0 94 1.0 95 1.0 96 0.5 97 1.0 98 2.0 99 1.0 100 6.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.005 145-149 0.015 150-151 0.0125 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 60.075 #Duplication Level Percentage of deduplicated Percentage of total 1 63.670411985018724 38.25 2 20.765709529754474 24.95 3 8.40615896795672 15.15 4 3.5788597586350397 8.6 5 2.2888056595921764 6.875000000000001 6 0.41614648356221395 1.5 7 0.5409904286308781 2.275 8 0.04161464835622139 0.2 9 0.12484394506866417 0.675 >10 0.16645859342488556 1.525 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG 26 0.65 No Hit GCACCATCTTTGATCCATTCACTTGGGAACCCTTCAAGGACTTCCCTTTC 13 0.325 No Hit GAGAGTCAGTCCTGTCTTTCGATCTTCTCCCCCCTTTATTTTATTTTATA 12 0.3 No Hit GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGT 10 0.25 No Hit ATCGATCACTCTCACAAGGGAGCACCAGAAGGTTGTTGACTACAAGCCAT 9 0.22499999999999998 No Hit GTTATGTTTTTATTATTAATTTGGTTCCACTTTATGTGTTGGTGTTGTTG 9 0.22499999999999998 No Hit GGCATGATGCATTTGAGACTATACCAAGAACAGAACAAGTGCAGCTTCTG 9 0.22499999999999998 No Hit AGACTAGTTATGTTCCTTGAACCCATTTTGTGTAGTGGCTACTTCGACAA 8 0.2 No Hit TGAATAGGTTGTACTACTTATGTAGCTTTTGAGCTGAATTCAACTTATGT 7 0.17500000000000002 No Hit ATGCCGTTCTAGCTCAAGCATGCCATTGGACTAGTACGAGCTCTCGCTAT 7 0.17500000000000002 No Hit TGTTTACTTGAGTCATCGAAAGAATGCTTTGAGAATGATGAGGTTAGCAT 7 0.17500000000000002 No Hit CTTTAAACAGCTCTCATTCAAGATGCAAATCTTCGTCAAGACTCTTACTG 7 0.17500000000000002 No Hit AGTATTTGAAAGATTGCTACATTGAAGGCTACGTGGACTTTATTTTTGGG 7 0.17500000000000002 No Hit CTTCTTCAGTGAACATACCATCATCTTCAGTGAACATATCATCTTCTTCA 7 0.17500000000000002 No Hit GTCTGGTAGTCCTTATCATGATTTGTCTCAAAGGTTACTAGATTTTTTTT 7 0.17500000000000002 No Hit GTAGCAAGATGGAGGAGGTTGACTAAACCCCGGCACTGCAACAGGCCCGT 7 0.17500000000000002 No Hit AGCAGTTCTGACTGTTTTCCTACACCAAGGAGTGAAGGTGAAATATTGTC 7 0.17500000000000002 No Hit GTTCAAGTGAAAGCATTTTCTTGATAAGCTATCAAATTAAGCGAGCGACG 7 0.17500000000000002 No Hit GGGATGGATTATTCCTCCGAACAGCTCTTACTACGAGGAATGGACCAGCC 7 0.17500000000000002 No Hit CCAATATATCTGATTGGGTTAAGTAAAAATCAACGAGCAAAGTCTAGGAA 7 0.17500000000000002 No Hit CACCGGGTAGCACAGGCCAAGGAAGAATAAAATGGGTGCAAAAGAACTAC 7 0.17500000000000002 No Hit AATCAGAAGAACTGGAGGAAGATCAAGAGTTGTTTGATTGATGCTAAAGT 6 0.15 No Hit ACACAAACATCTAATTCTACAATAACCTAGCTTTTACGGTGCAAACCTTG 6 0.15 No Hit GCAAGAAACCAATTGTAAAGAAGGTGGTCATGGAGTATTGTTATGAACCT 6 0.15 No Hit GACCGAGAACAATATAACAAAGCAGATTTCCCTGAGGAATATGATAATGC 6 0.15 No Hit GTGTGGCTTAAGACCCTGCCCCCGGCTCCCCGAAACTGAGTGATCTAGAG 6 0.15 No Hit GAATTCTTGACTGCTCTTTTGCTTTTTAGTTGCTCTGTCTAAAGTGAATT 6 0.15 No Hit GCAGTTATGTACAGGAAAATTCGTAAAGGAGAATATCAATTGCCTTCTTG 6 0.15 No Hit TTTAGTTGGCCCAGACAAAGTTGTTCCTCTTCTCTTTTTTTTATTGATGG 6 0.15 No Hit GGAATATCTTAGGCCAATCTCTTTACCAGTTTATGGTAATATGGCACCTT 6 0.15 No Hit AGTGATGCAAGCTTGTTTTCGAGTTCATTGCCTGTCCTTTCACATGAAAA 6 0.15 No Hit GAATAATAAGGAGAGGGGTGAAGATGATGGGTCGACAAGAGGGAAAAAGG 5 0.125 No Hit CACATGCAAATGGAACTACAGGCTGGGAGCTGGCGCTTGTCACGGCTCCT 5 0.125 No Hit GAAACATGAGTCCAGAGGCAGCTATGGAGCAGTATATTGTCCTTGTATCT 5 0.125 No Hit CACAGATTTAGGATCGATCGAGTCAGCTTTCTTGCTTGCCTGCTTGCTAG 5 0.125 No Hit TGTACGGAGTGAAAATATTGGATGATTTGTTGATTCTGATTTGGAAAGAA 5 0.125 No Hit AGGTTATTCAACTTCAAGGTGACCAGCGCAAGAATGTATCGACCTTCCTT 5 0.125 No Hit GTAGAAGAGCTAGAGCCACTAGAAAGCGGAGATAGAGAACTTGACGAGGG 5 0.125 No Hit GCCTGAATATGTACGTGAACGTACCGATGCTTTGGATGGTGCTGGTAACA 5 0.125 No Hit GGTGTTTAAGCGTACAATGGGGAGTGGAGATAAAAATCATTTGAGTTTCC 5 0.125 No Hit AATCTTTGCCTCCTCCTGTCTGAGTTCTTGACCTCAGACTTTCTTTAGTT 5 0.125 No Hit GCTCAAATGCTCATCCTAATTTTGGCAATAACTTGATTCAAGAGTGCATG 5 0.125 No Hit GCCAAACTCCGGCAAGGAATCTGATGGTAGTGGTGGTAGCGTGATGAGGT 5 0.125 No Hit GCTACTTGTCTTGTGTATTGACTGACTAATTGTAGTTAGCATGGCACGTC 5 0.125 No Hit GTTGAATATGAGAAGAAGATGAAAGCTTACAACAAGGAGCAGGCTGAAGG 5 0.125 No Hit GTTGGTGCTGGTGCTGGGAATCCTCTATTTACTACTGACACGGCAGCTGC 5 0.125 No Hit GCTTGATGCGGCATACCAAGAGGCTGAGCAGAAATCTGGTGGCTGTCCTG 5 0.125 No Hit GGAAACTCGCCTTCCTTCTTCCCTTTAAAAAAATTCCCAAAAATGGCAAC 5 0.125 No Hit GTCACAGTTGATTAAACAAGATATATCTTCCTTCGATGGTCTTCAGCACT 5 0.125 No Hit CATCGTTGTCATCTTTAAAATTAAGAAGAAGAAGAGTCAAACGTAGGAAA 5 0.125 No Hit GTCGGAAGAATCTCAAGGAAAAGCATGAATTATCTGGACCTTCTTCTAGC 5 0.125 No Hit GTCTCTTTCACGCAATGTAATCTGCTCCAAGTGCAAGGGTAAAGGTTCCA 5 0.125 No Hit GCTCAGCAGCTCGCCGCTGGTACCGGTAACGGTGTTGTCCAGAACGGTGC 5 0.125 No Hit CCTCAATGTTTGTGATAATCTCGCTGATCACATGATCGGTAACGTTTATG 5 0.125 No Hit CTAATTTGTACTTGCAATAATTTGCTTCTGTGAGCTTCTCTTAGTACTGC 5 0.125 No Hit GACTCAACTTGGATGGCCTCCTCTTTCCCTCAGCTCAACCTTTTCATCAG 5 0.125 No Hit GAGGAGAGGGCTAATGTTTTCCATGTTGAGGTTGAGTAGGTTGCAGGGGA 5 0.125 No Hit TGCAACAGCAGCTCTCAAGTTGTGAGAATCTATCTTAGATAATTGCTAGG 5 0.125 No Hit CTGATGACGCTATTAACAAGGCTGATCACTTGAGGAAATCGTCTCCAATG 5 0.125 No Hit AAGCAGGATTCTTTGACTTGATCAATATGTATTTTCCAATGGTGTATGAT 5 0.125 No Hit GAAGGCTACTGGTTTAATTTGATATTTGATGTTGAAGGTCACTTAGGATA 5 0.125 No Hit GCGACAACAAACAGAGGGGGAGGGAAAGCAGGGGTCGCAGACAGGACAGG 5 0.125 No Hit AGCAAAGGATCTCAGAAACTGATTCCAGGGCAGAGCCTCAAACAATCAGG 5 0.125 No Hit GCTGGGGGAAGGTTATGGTATGCTTGGTCAGGCTGGGAATGGCAAGCTGC 5 0.125 No Hit ATTGAAGACATCAGACCTGCCGGTGGAATCAAGAAGTTCCGATCTGCTGC 5 0.125 No Hit TGACAGAGGCCGTGAGGCGGAGGCCTTATAGCGTTGTGCTGTTTGATGAA 5 0.125 No Hit GTCTGGTCTCTCCTCGCCCTCTCACTGGAGCAGTAGGATGGCAGATCTGA 5 0.125 No Hit GTTGTATTCTATAGCTCCATTATGGCCGTTATATGGAATCATTGGAGTGC 5 0.125 No Hit GAATGTGGATACAAGTGAGATATTCCTTGATATTGCGTGCTATTTTGTTG 5 0.125 No Hit CAACAATGGCTGGTTTCTTTGTTAATTCTGGTGAGAAATTAACTATCAAA 5 0.125 No Hit CGAATGTCAGAGGTATTAGCAACGCTGGAGAACATTGAATCCCCAAAAGG 5 0.125 No Hit CCGGATATCTATACAGATGGGAGGATTTCAGTTCAAGGGATTGATGGGGT 5 0.125 No Hit ATTTATGATGTGATACCGATATACTTGGATATCTTTAGAGGTGATTCCAG 5 0.125 No Hit TGTCACATCTGGTCCTCTAGCTTCCCTGAAGATTGAAATAGTTCCCCTTG 5 0.125 No Hit GATGTTCGCAAATATGTGAACACATACCGCAGGACCTTCACAACAAAATC 5 0.125 No Hit GGAGAAATCCCTGAAAAGGAAACAAGAAGAATGGAACTTTGACCAATTAA 5 0.125 No Hit GAGATTCTGGGGTGACGCCCTTGACTCCTTCTGACAATGCTTCTATGTAT 5 0.125 No Hit GGATTCACATCTGCCGATGTGGGTCTTGATGCCGACAATTGCAAGGTTCT 5 0.125 No Hit GCTCTGTTCACTTTGGTCCGACATTCCTCACCTCTCCCCACCTGAGCTCT 5 0.125 No Hit GCCAAAGAAGATTCTGAGGCCTACTCTGCATGGGCTCCTGATCCAGTTAC 5 0.125 No Hit GCCCTAACTTAATGGACGGGAGGTATCCCAATAGGAGGTTTCCTCCTATG 5 0.125 No Hit TGAGTTCCATGCAGCTTTCTTTTGAAGATGTGCAGATGTTCCTTTTCAAA 5 0.125 No Hit GGGGATGGGAATGGAAAGTCAGAGAAAGCTGGTTGATGAGAATGAGAAAC 5 0.125 No Hit GAAAAATACCAAAAAGAGATGCTGTTATTGTGGAAACACACCATACAGAT 5 0.125 No Hit GCTCCACCATACTCCTGATGAAAGTGTTGATGTGAACAACCCAAGAGATG 5 0.125 No Hit TCTTCACCTCAAACTTTCTTTATTTACCGTTTGATCTCAACTTTTTCTTT 5 0.125 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0125 0.0 0.0 0.0 0.0 56-57 0.025 0.0 0.0 0.0 0.0 58-59 0.05 0.0 0.0 0.0 0.0 60-61 0.05 0.0 0.0 0.0 0.0 62-63 0.05 0.0 0.0 0.0 0.0 64-65 0.05 0.0 0.0 0.0 0.0 66-67 0.05 0.0 0.0 0.0 0.0 68-69 0.0625 0.0 0.0 0.0 0.0 70-71 0.075 0.0 0.0 0.0 0.0 72-73 0.075 0.0 0.0 0.0 0.0 74-75 0.075 0.0 0.0 0.0 0.0 76-77 0.1125 0.0 0.0 0.0 0.0 78-79 0.125 0.0 0.0 0.0 0.0 80-81 0.15 0.0 0.0 0.0 0.0 82-83 0.2 0.0 0.0 0.0 0.0 84-85 0.3125 0.0 0.0 0.0 0.0 86-87 0.35 0.0 0.0 0.0 0.0 88-89 0.4625 0.0 0.0 0.0 0.0 90-91 0.65 0.0 0.0 0.0 0.0 92-93 0.725 0.0 0.0 0.0 0.0 94-95 0.7875 0.0 0.0 0.0 0.0 96-97 0.95 0.0 0.0 0.0 0.0 98-99 1.1124999999999998 0.0 0.0 0.0 0.0 100-101 1.3875000000000002 0.0 0.0 0.0 0.0 102-103 1.5750000000000002 0.0 0.0 0.0 0.0 104-105 1.9500000000000002 0.0 0.0 0.0 0.0 106-107 2.3375000000000004 0.0 0.0 0.0 0.0 108-109 2.65 0.0 0.0 0.0 0.0 110-111 3.1 0.0 0.0 0.0 0.0 112-113 3.6375 0.0 0.0 0.0 0.0 114-115 4.3 0.0 0.0 0.0 0.0 116-117 4.7 0.0 0.0 0.0 0.0 118-119 5.0375 0.0 0.0 0.0 0.0 120-121 5.637499999999999 0.0 0.0 0.0 0.0 122-123 6.4375 0.0 0.0 0.0 0.0 124-125 6.9625 0.0 0.0 0.0 0.0 126-127 7.7875 0.0 0.0 0.0 0.0 128-129 8.55 0.0 0.0 0.0 0.0 130-131 9.125 0.0 0.0 0.0 0.0 132-133 9.8875 0.0 0.0 0.0 0.0 134-135 10.7 0.0 0.0 0.0 0.0 136-137 11.712499999999999 0.0 0.0 0.0 0.0 138-139 13.2 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GCCCTAA 10 0.006830828 145.0 7 CCTAACT 10 0.006830828 145.0 9 CTGAAAG 10 0.006830828 145.0 1 GGGGGTG 10 0.006830828 145.0 3 CCCTAAC 10 0.006830828 145.0 8 AGCCCTA 10 0.006830828 145.0 6 >>END_MODULE Read 756547 spots for SRR26075375.sra Written 756547 spots for SRR26075375.sra Read 756547 spots for SRR26075375.sra Written 756547 spots for SRR26075375.sra Read 756547 spots for SRR26075375.sra Written 756547 spots for SRR26075375.sra Read 756547 spots for SRR26075375.sra Written 756547 spots for SRR26075375.sra Read 756547 spots for SRR26075375.sra Written 756547 spots for SRR26075375.sra Read 756547 spots for SRR26075375.sra Written 756547 spots for SRR26075375.sra Read 756547 spots for SRR26075375.sra Written 756547 spots for SRR26075375.sra Read 756547 spots for SRR26075375.sra Written 756547 spots for SRR26075375.sra Read 756547 spots for SRR26075375.sra Written 756547 spots for SRR26075375.sra Read 756547 spots for SRR26075375.sra Written 756547 spots for SRR26075375.sra Read 756547 spots for SRR26075375.sra Written 756547 spots for SRR26075375.sra Read 756547 spots for SRR26075375.sra Written 756547 spots for SRR26075375.sra Read 756547 spots for SRR26075375.sra Written 756547 spots for SRR26075375.sra Read 756562 spots for SRR26075375.sra Written 756562 spots for SRR26075375.sra Read 756547 spots for SRR26075375.sra Written 756547 spots for SRR26075375.sra Read 756547 spots for SRR26075375.sra Written 756547 spots for SRR26075375.sra Read 756547 spots for SRR26075375.sra Written 756547 spots for SRR26075375.sra Read 756547 spots for SRR26075375.sra Written 756547 spots for SRR26075375.sra Read 756547 spots for SRR26075375.sra Written 756547 spots for SRR26075375.sra Read 756547 spots for SRR26075375.sra Written 756547 spots for SRR26075375.sra SRR ids: ['SRR26075375.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_bjk4itc0 SRR26075375.sra spots: 15130955 blocks: [[1, 756547], [756548, 1513094], [1513095, 2269641], [2269642, 3026188], [3026189, 3782735], [3782736, 4539282], [4539283, 5295829], [5295830, 6052376], [6052377, 6808923], [6808924, 7565470], [7565471, 8322017], [8322018, 9078564], [9078565, 9835111], [9835112, 10591658], [10591659, 11348205], [11348206, 12104752], [12104753, 12861299], [12861300, 13617846], [13617847, 14374393], [14374394, 15130955]] SRR26075375 file size 5581479 SRR26075375 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o SRR26075375 SRR26075375_1.fastq SRR26075375_2.fastq Input file: SRR26075375_1.fastq Paired file: SRR26075375_2.fastq trimmed: SRR26075375-trimmed-pair1.fastq, SRR26075375-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Fri Apr 11 11:42:21 2025 >> started Fri Apr 11 11:42:37 2025 >> done (16.396s) 15130955 read pairs processed; of these: 45 ( 0.00%) short read pairs filtered out after trimming by size control 11154 ( 0.07%) empty read pairs filtered out after trimming by size control 15119756 (99.93%) read pairs available; of these: 2696323 (17.83%) trimmed read pairs available after processing 12423433 (82.17%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 18 10 0.00% 19 5 0.00% 20 7 0.00% 21 14 0.00% 22 9 0.00% 23 7 0.00% 24 10 0.00% 25 5 0.00% 26 11 0.00% 27 23 0.00% 28 18 0.00% 29 21 0.00% 30 11 0.00% 31 23 0.00% 32 18 0.00% 33 13 0.00% 34 12 0.00% 35 17 0.00% 36 18 0.00% 37 25 0.00% 38 56 0.00% 39 25 0.00% 40 40 0.00% 41 39 0.00% 42 46 0.00% 43 52 0.00% 44 40 0.00% 45 55 0.00% 46 65 0.00% 47 72 0.00% 48 94 0.00% 49 103 0.00% 50 121 0.00% 51 136 0.00% 52 128 0.00% 53 159 0.00% 54 135 0.00% 55 216 0.00% 56 215 0.00% 57 256 0.00% 58 320 0.00% 59 347 0.00% 60 360 0.00% 61 420 0.00% 62 518 0.00% 63 634 0.00% 64 622 0.00% 65 736 0.00% 66 816 0.01% 67 858 0.01% 68 999 0.01% 69 1203 0.01% 70 1420 0.01% 71 1734 0.01% 72 1835 0.01% 73 2133 0.01% 74 2281 0.02% 75 2591 0.02% 76 2997 0.02% 77 3216 0.02% 78 3718 0.02% 79 4053 0.03% 80 4658 0.03% 81 5308 0.04% 82 6166 0.04% 83 6634 0.04% 84 7477 0.05% 85 7906 0.05% 86 8400 0.06% 87 8696 0.06% 88 9601 0.06% 89 10406 0.07% 90 11058 0.07% 91 12074 0.08% 92 13695 0.09% 93 14666 0.10% 94 15634 0.10% 95 16640 0.11% 96 17211 0.11% 97 18051 0.12% 98 18415 0.12% 99 19885 0.13% 100 21122 0.14% 101 22117 0.15% 102 23622 0.16% 103 25254 0.17% 104 26020 0.17% 105 27318 0.18% 106 28678 0.19% 107 28531 0.19% 108 29298 0.19% 109 30093 0.20% 110 30631 0.20% 111 33239 0.22% 112 34301 0.23% 113 35561 0.24% 114 37360 0.25% 115 38924 0.26% 116 39946 0.26% 117 40059 0.26% 118 41254 0.27% 119 41011 0.27% 120 42372 0.28% 121 43281 0.29% 122 44658 0.30% 123 46470 0.31% 124 48307 0.32% 125 49136 0.32% 126 50328 0.33% 127 51598 0.34% 128 51061 0.34% 129 51430 0.34% 130 52520 0.35% 131 53762 0.36% 132 54844 0.36% 133 57014 0.38% 134 58584 0.39% 135 59712 0.39% 136 60356 0.40% 137 59566 0.39% 138 61531 0.41% 139 61832 0.41% 140 61285 0.41% 141 63268 0.42% 142 64047 0.42% 143 64660 0.43% 144 66661 0.44% 145 68410 0.45% 146 68074 0.45% 147 68213 0.45% 148 68896 0.46% 149 70232 0.46% 150 71154 0.47% 151 12423433 82.17% 15119756 reads passed initial QC criterion=sequence-density sequence-density=0.23 sequence-density-rank=1 fanout-score=3.36 fanout-score-rank=33 prefix-density=0.23 prefix-fanout=3.3 sequence=GCAAGCTGGTTGCTGTCCAACCA criterion=fanout-score sequence-density=0.06 sequence-density-rank=41 fanout-score=61.58 fanout-score-rank=1 prefix-density=0.33 prefix-fanout=11.8 sequence=CAACAGCAACACAAGGCAGCACAACATCCCTTCCAGAAGCCATCACCCCTGGATTTAGTCTTCACAGGAATAGGATTTTGCTGGTCGGATTGACCATCCATGGTTGGATAGCCAGCTGGTGGTGGGCCTGAATATGCACTAGGATAAGTTGATGTTGGTGGGGGAGGATATGCCACTGTAGCTTGATTCTGACTCATGGTTGAAGAAGCGAGTTGAGAAGCACGAACAAATGAGAGCTATGGAAGAATGGAGATAAGGACGAGAGAAGTTCGGTTGTGGT criterion=sequence-density sequence-density=0.27 sequence-density-rank=1 fanout-score=2.48 fanout-score-rank=34 prefix-density=0.30 prefix-fanout=2.2 sequence=AAGATCCAGGACAAGGAAGG criterion=fanout-score sequence-density=0.13 sequence-density-rank=19 fanout-score=79.44 fanout-score-rank=1 prefix-density=0.62 prefix-fanout=16.5 sequence=GATGATGATGCT SRR26075375 testing PE reads STAR mapping to Ensembl genome Started job on | Apr 11 11:43:26 Started mapping on | Apr 11 11:43:26 Finished on | Apr 11 11:46:30 Mapping speed, Million of reads per hour | 295.82 Number of input reads | 15119756 Average input read length | 292 UNIQUE READS: Uniquely mapped reads number | 13097029 Uniquely mapped reads % | 86.62% Average mapped length | 291.04 Number of splices: Total | 10098895 Number of splices: Annotated (sjdb) | 9802828 Number of splices: GT/AG | 9923813 Number of splices: GC/AG | 122433 Number of splices: AT/AC | 11240 Number of splices: Non-canonical | 41409 Mismatch rate per base, % | 0.41% Deletion rate per base | 0.03% Deletion average length | 2.90 Insertion rate per base | 0.02% Insertion average length | 2.63 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 404320 % of reads mapped to multiple loci | 2.67% Number of reads mapped to too many loci | 37549 % of reads mapped to too many loci | 0.25% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 10.05% % of reads unmapped: other | 0.41% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 1618407 1618407 1618407 N_multimapping 404320 404320 404320 N_noFeature 361853 12955749 442448 N_ambiguous 148329 846 87148 UnstrandedReadsAssigned:12586847 PositiveStrandReadsAssigned:140434 NegativeStrandReadsAssigned:12567433 Dataset is classified negative stranded MeadianReadLen=151 20thPercentileLength=151 echo kmer=147 SRR26075375 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,400 [index] number of k-mers: 62,057,036 [index] number of equivalence classes: 130,681 [quant] running in paired-end mode [quant] will process pair 1: SRR26075375-trimmed-pair1.fastq SRR26075375-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 15,119,756 reads, 12,837,987 reads pseudoaligned [quant] estimated average fragment length: 205.373 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,160 rounds 52401 SRR26075375.ke.tsv 34699 SRR26075375.se.tsv 87100 total ==> SRR26075375.ke.tsv <== target_id length eff_length est_counts tpm Potri.005G200100.1.v4.1 2018 1813.63 3132 114.184 Potri.005G024800.1.v4.1 1035 830.627 7833 623.524 Potri.004G059700.1.v4.1 961 756.632 4 0.349548 Potri.007G009000.2.v4.1 1416 1211.63 0 0 Potri.003G141000.2.v4.1 2943 2738.63 569 13.7376 Potri.016G087400.1.v4.1 270 92.3437 826 591.431 Potri.015G069301.1.v4.1 564 360.865 0 0 Potri.010G195200.1.v4.1 1773 1568.63 27 1.13809 Potri.012G127500.1.v4.1 977 772.627 2323 198.797 ==> SRR26075375.se.tsv <== Potri.001G166300.v4.1 0 Potri.001G448400.v4.1 26 Potri.001G233950.v4.1 0 Potri.001G122700.v4.1 42 Potri.001G212900.v4.1 0 Potri.001G182400.v4.1 0 Potri.001G256600.v4.1 0 Potri.001G040500.v4.1 8 Potri.001G416900.v4.1 0 Potri.001G452600.v4.1 102 SRR26075375 completed mapping pipeline successfully