Starting /dee2/code/volunteer_pipeline.sh SRR26075376 current disk space = 3052203339776 free memory = 1464833456 SRR26075376 SRAfilesize f83031a5f1caff464d4b58f8caeddad3 SRR26075376.sra SRR26075376.sra file validated SRR26075376 is paired end SRR26075376 is conventional basespace SRR26075376 read1 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR26075376_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.486 37.0 37.0 37.0 37.0 37.0 2 36.5445 37.0 37.0 37.0 37.0 37.0 3 36.6605 37.0 37.0 37.0 37.0 37.0 4 36.7185 37.0 37.0 37.0 37.0 37.0 5 36.677 37.0 37.0 37.0 37.0 37.0 6 36.554 37.0 37.0 37.0 37.0 37.0 7 36.7045 37.0 37.0 37.0 37.0 37.0 8 36.6185 37.0 37.0 37.0 37.0 37.0 9 36.723 37.0 37.0 37.0 37.0 37.0 10-14 36.681599999999996 37.0 37.0 37.0 37.0 37.0 15-19 36.6561 37.0 37.0 37.0 37.0 37.0 20-24 36.5598 37.0 37.0 37.0 37.0 37.0 25-29 36.4748 37.0 37.0 37.0 37.0 37.0 30-34 36.485400000000006 37.0 37.0 37.0 37.0 37.0 35-39 36.4009 37.0 37.0 37.0 37.0 37.0 40-44 36.3436 37.0 37.0 37.0 37.0 37.0 45-49 36.0609 37.0 37.0 37.0 37.0 37.0 50-54 36.187400000000004 37.0 37.0 37.0 37.0 37.0 55-59 35.897499999999994 37.0 37.0 37.0 37.0 37.0 60-64 35.820100000000004 37.0 37.0 37.0 37.0 37.0 65-69 35.764700000000005 37.0 37.0 37.0 37.0 37.0 70-74 35.9981 37.0 37.0 37.0 37.0 37.0 75-79 36.0552 37.0 37.0 37.0 37.0 37.0 80-84 36.0767 37.0 37.0 37.0 37.0 37.0 85-89 35.9536 37.0 37.0 37.0 37.0 37.0 90-94 35.9182 37.0 37.0 37.0 37.0 37.0 95-99 35.8797 37.0 37.0 37.0 37.0 37.0 100-104 35.8477 37.0 37.0 37.0 37.0 37.0 105-109 35.779199999999996 37.0 37.0 37.0 37.0 37.0 110-114 35.6528 37.0 37.0 37.0 37.0 37.0 115-119 35.6078 37.0 37.0 37.0 37.0 37.0 120-124 35.719699999999996 37.0 37.0 37.0 37.0 37.0 125-129 35.5954 37.0 37.0 37.0 37.0 37.0 130-134 35.4302 37.0 37.0 37.0 34.6 37.0 135-139 35.2644 37.0 37.0 37.0 32.2 37.0 140-144 35.1922 37.0 37.0 37.0 27.4 37.0 145-149 35.1221 37.0 37.0 37.0 27.4 37.0 150-151 34.92375 37.0 37.0 37.0 25.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.0 1101 2 0.0 1101 3 0.0 1101 4 0.0 1101 5 0.0 1101 6 0.0 1101 7 0.0 1101 8 0.0 1101 9 0.0 1101 10-14 0.0 1101 15-19 0.0 1101 20-24 0.0 1101 25-29 0.0 1101 30-34 0.0 1101 35-39 0.0 1101 40-44 0.0 1101 45-49 0.0 1101 50-54 0.0 1101 55-59 0.0 1101 60-64 0.0 1101 65-69 0.0 1101 70-74 0.0 1101 75-79 0.0 1101 80-84 0.0 1101 85-89 0.0 1101 90-94 0.0 1101 95-99 0.0 1101 100-104 0.0 1101 105-109 0.0 1101 110-114 0.0 1101 115-119 0.0 1101 120-124 0.0 1101 125-129 0.0 1101 130-134 0.0 1101 135-139 0.0 1101 140-144 0.0 1101 145-149 0.0 1101 150-151 0.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 17 1.0 18 0.0 19 1.0 20 2.0 21 2.0 22 1.0 23 2.0 24 2.0 25 2.0 26 14.0 27 13.0 28 15.0 29 28.0 30 36.0 31 52.0 32 57.0 33 124.0 34 181.0 35 407.0 36 2846.0 37 214.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 38.63750628456511 18.149824032176973 7.6420311714429365 35.570638511814984 2 19.275000000000002 17.1 34.949999999999996 28.675 3 17.4 17.8 29.15 35.65 4 19.175 24.975 25.35 30.5 5 21.725 29.925 26.625 21.725 6 21.575 33.050000000000004 24.525 20.849999999999998 7 15.299999999999999 30.95 38.425 15.325 8 16.625 29.125 32.574999999999996 21.675 9 19.725 24.575 33.875 21.825 10-14 19.8 30.895 26.479999999999997 22.825 15-19 19.33 29.049999999999997 27.125 24.495 20-24 18.425 29.805 27.55 24.22 25-29 19.29 29.285 27.1 24.325 30-34 18.34 29.34 26.515 25.805 35-39 20.05 28.375 26.784999999999997 24.79 40-44 19.765 28.815 28.134999999999998 23.285 45-49 20.244999999999997 28.744999999999997 26.87 24.14 50-54 19.85 27.93 27.05 25.169999999999998 55-59 19.555 28.185 27.145000000000003 25.115 60-64 20.575 26.900000000000002 28.050000000000004 24.474999999999998 65-69 20.415 28.494999999999997 27.735 23.355 70-74 22.515 27.49 26.205000000000002 23.79 75-79 21.525 28.155 26.185000000000002 24.135 80-84 21.515 27.92 26.919999999999998 23.645 85-89 21.505 27.139999999999997 27.384999999999998 23.97 90-94 21.4 27.229999999999997 26.779999999999998 24.59 95-99 21.87 26.484999999999996 27.095000000000002 24.55 100-104 21.305 27.229999999999997 26.875 24.59 105-109 21.740000000000002 27.26 26.245 24.755 110-114 22.025 27.345000000000002 26.72 23.91 115-119 22.67 27.215 25.919999999999998 24.195 120-124 22.105 27.224999999999998 26.784999999999997 23.885 125-129 22.58 26.490000000000002 26.125 24.805 130-134 23.73 26.155 26.265 23.849999999999998 135-139 22.715 26.715 25.66 24.91 140-144 23.26 26.235000000000003 26.33 24.175 145-149 23.215 26.415 25.69 24.68 150-151 23.075000000000003 25.85 26.35 24.725 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 1.5 19 3.0 20 1.5 21 0.0 22 1.0 23 3.5 24 5.0 25 7.0 26 7.0 27 9.0 28 18.5 29 17.0 30 23.5 31 39.0 32 40.5 33 48.5 34 53.5 35 77.5 36 97.0 37 102.0 38 130.0 39 140.0 40 168.5 41 197.5 42 199.0 43 207.5 44 229.0 45 237.0 46 231.5 47 226.0 48 249.0 49 244.0 50 189.0 51 150.5 52 117.5 53 92.5 54 76.5 55 59.5 56 44.0 57 37.5 58 26.0 59 18.5 60 15.0 61 11.0 62 7.5 63 7.0 64 11.0 65 15.0 66 22.0 67 26.5 68 21.5 69 17.0 70 10.5 71 3.5 72 3.0 73 3.0 74 0.5 75 0.0 76 0.0 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.5499999999999999 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150-151 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 62.975 #Duplication Level Percentage of deduplicated Percentage of total 1 66.41524414450178 41.825 2 19.76974990075427 24.9 3 8.257244938467647 15.6 4 3.2949583167923775 8.3 5 1.4291385470424771 4.5 6 0.4763795156808257 1.7999999999999998 7 0.19849146486701072 0.8750000000000001 8 0.0 0.0 9 0.0 0.0 >10 0.15879317189360856 2.1999999999999997 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCTCAGTTATCTCGTAT 48 1.2 TruSeq Adapter, Index 23 (97% over 37bp) GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCTCAGTTATCGCGTAT 20 0.5 TruSeq Adapter, Index 23 (97% over 37bp) ACAGGGAGACCGGAACTTCCAACTACTTGAGAAAAACAAACACACAAAAG 10 0.25 No Hit GCCAAAAGGTAGCTGCAAAGCTTGCTGCAGCATGGGCTGCCGGGTTTTGT 10 0.25 No Hit GGCAGAATCAGTAGATCCCTTGGGTAGGGCTGAGGGCACATCTTCATAGT 7 0.17500000000000002 No Hit TGGAGGCAGGTTGGACTTGAACTTAGCTCTGACAACACCGCTGTTTCCAT 7 0.17500000000000002 No Hit GCTCACTCGAGACACTACCACTAACAAAGACTCCCCTGCAACCTACTCAA 7 0.17500000000000002 No Hit CATACTTAAAATAGTTCCGAGAACATTATAGATTCCCTTTAACAATTGCC 7 0.17500000000000002 No Hit CTACTTTACATTACAGGGGATTTCCTTCAAACATGACAAGCCAAAACAGA 7 0.17500000000000002 No Hit GTCCGTTATCACGCTTCGTGTTCTCTCATCCATTCCCCAAATAAAGAAAA 6 0.15 No Hit ATTGTAAACAAGTTTTCAAATACATCCAAAAAGTTCTCATAATGGATTGC 6 0.15 No Hit CTCTTTTCCTGCGTTACCTTATTCCCTCTGCAATGAGGGCATTTATCCTT 6 0.15 No Hit CGCCACTTCACATTTCCTCCTCGAACCCTAATCCTCCTCACTGTCTTGTT 6 0.15 No Hit GCCTGTTTAGCAAGACTGCAAGCACGATCTGGAGAGTTGAGAATTTCATA 6 0.15 No Hit GGTTGTCTTTCAAATAACTTGAACATGATATCTTCCAATTCCCCACGATC 6 0.15 No Hit AGGATATTGGGGCCAATAACTCGTCTTAAAGATGTGATTGCGGGTGGCCA 6 0.15 No Hit GTTATACTAACAAAACCTGCGTTCCATAAGAGTGTTTATTATGGCATGTG 6 0.15 No Hit GTGGGACTTGGCGTGATCGAAGGCTGCAACACAGAGGTGAGAAGCCATGC 6 0.15 No Hit GAAGCGGTGACGTCCGTGTGGGATGTCTTTTCTGAAGTCAGGAGGTTCAG 6 0.15 No Hit CAGGAATTGAGATCAACTCACGGCAGTCACCTATAATCAATTGCACCAGA 6 0.15 No Hit TTCTTGTAAAACCTGTCAGAAATTAACTTGACACCAGAAATATCACCAAA 6 0.15 No Hit GCCTACTAACTTATCTCACTTAATCTGTCACATTGGCCCCGGGTCATAAT 5 0.125 No Hit TGGCTGTAGGAACTCTGAATGAAGCTGCTGGTGCTTGAGGAAGAATATTA 5 0.125 No Hit GATCAAATAACAAAGAGCGTGACGCGACCAAACCCATAGCCACCACCATC 5 0.125 No Hit GTCTTCATTGCCCAGTAAAGATTTAGTAGAGTTCATAGTCCATCACAAAT 5 0.125 No Hit CCCTCATGTAACATGAAAAGTTCATACAAGCAAAGAAGCTTGTTTACACA 5 0.125 No Hit CCTGGATTCATTTAATTTTCAGCCTCTTTCCTATTCCCATAATTCATCTC 5 0.125 No Hit GTTGTCTCAACAGATTAATCATTCGACTACCAGTTTGCTCTGTTGCCAAC 5 0.125 No Hit GGCATCCAATAGAGGCTCCCCCTCAAAAGGCTCATAATCTTCAATCATCT 5 0.125 No Hit ATTCATTTACATATTGGCATGCAGTTCACATTTCAACACAGGCTAAAGAT 5 0.125 No Hit GTGATTTTTCGGCAATCGAAAATCCTCTGTGAAATCCTCAATAAGCGAGT 5 0.125 No Hit GTGTGGTGGACCGGATGGTGGTGCATGCGGACCTGGGGGAGGAGGAGAAT 5 0.125 No Hit GGCCTAATTGAGCGCAAGTTAATCACCTCAGCGCTGATTCCTTCCTTTGC 5 0.125 No Hit CATAAAGAGATGGTAGTCACACATGATAATATGAAAAAAGAAATCTGAAA 5 0.125 No Hit GTTGAACCTAGGATTGTTGCGGTTGTTGTTAGAGCTATAAACGTTAGGGT 5 0.125 No Hit AGCTCTAAGAGGAGGTTCAAAATGCTTGCTGGCAACATCAGGAGGTGGTG 5 0.125 No Hit CCTGGATCTTGGCCTTGACATTGTCGATGGTATCAGAGCTCTCAACCTCC 5 0.125 No Hit ATGAGCCACAGAATAAGACGCCCACCAAACTGCATTAGATGGTGCATAAG 5 0.125 No Hit TTTTTTACAAAAGAGGTGACAGATAAGTTACTAGAGAAACAGCTACTGTT 5 0.125 No Hit GCACACGGATTGCTACCGTTAAGACGGTTAGTGATTACAAACAAGCCAGT 5 0.125 No Hit TCATAAATGTCATTGCTCCATACCTGAATAATGTCACCAACATTGATGAT 5 0.125 No Hit CAGCTGGTTGGACACATCAGAATGGTCCTTGCGAGTCATCCCCTCACCAA 5 0.125 No Hit GTCACAGAAAATACAAGTTAATTTCAGTTATTTATCAGCACAGCACAAGT 5 0.125 No Hit CCATAAAAGAAAACAGAGAACCCACCAAAAGCTTCTAAAGCTGGCCTATT 5 0.125 No Hit GAATCAAGAAACATGGAAATATAAAATTTCTTCCGACAAGGGGTGAAGGT 5 0.125 No Hit GTCACCAATCCTTGCAATTTGATTAATGCTAAGAGGAACATGAGGTATAA 5 0.125 No Hit AAATGATCTAAAAGAATGTTCAAGCTAAACATGGATCCGTTGATTTAGGA 5 0.125 No Hit CGGGCAGGCATTTATCTGAATAGCATTTTCTTAACTTCATATCCTTGAGC 5 0.125 No Hit GTCGTGTCTGCAATCATGGAGGTGACAACATAAGGATCCATATTGGAAGC 5 0.125 No Hit GAAAGACACCAAATTTGGACACCAACTTGCATTTACAATTTTCAGAGATG 5 0.125 No Hit AACACAGATACGCGAAATCAGAAAAATAGTCATTACATCGGCGAAAGTAC 5 0.125 No Hit CCCTGCTTGGGCTTGCTCCGCGGAAGTGTTCGATTACAGCACGTAAATCA 5 0.125 No Hit ACCGCCAATTATTGCTTATACATAGTAGAGCAGAATCTTGTCTTCAGATA 5 0.125 No Hit ACCAGGATGTGGTGATGGGAAGCCAGAAAACTTTCTTGGGTGCTTCATTT 5 0.125 No Hit AGATAGTAGTCCATGATCATACGGCTTGCACAGGCAGGCAAAAACTTGGC 5 0.125 No Hit TTTGCCTCAGTTTCATGCTCCAATCGCTTCACATTAGCACCAGAGTCCCC 5 0.125 No Hit CTAGACCCGAGATCCAGCACGCCGTCGCATGGAGGCATTTGAAACGGAAA 5 0.125 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.025 0.0 0.0 0.0 0.0 52-53 0.025 0.0 0.0 0.0 0.0 54-55 0.025 0.0 0.0 0.0 0.0 56-57 0.05 0.0125 0.0 0.0 0.0 58-59 0.05 0.025 0.0 0.0 0.0 60-61 0.07500000000000001 0.025 0.0 0.0 0.0 62-63 0.175 0.025 0.0 0.0 0.0 64-65 0.175 0.025 0.0 0.0 0.0 66-67 0.175 0.025 0.0 0.0 0.0 68-69 0.175 0.025 0.0 0.0 0.0 70-71 0.175 0.025 0.0 0.0 0.0 72-73 0.175 0.025 0.0 0.0 0.0 74-75 0.21250000000000002 0.025 0.0 0.0 0.0 76-77 0.2375 0.025 0.0 0.0 0.0 78-79 0.25 0.025 0.0 0.0 0.0 80-81 0.35 0.025 0.0 0.0 0.0 82-83 0.375 0.025 0.0 0.0 0.0 84-85 0.475 0.025 0.0 0.0 0.0 86-87 0.625 0.025 0.0 0.0 0.0 88-89 0.8999999999999999 0.025 0.0 0.0 0.0 90-91 1.0375 0.025 0.0 0.0 0.0 92-93 1.2375 0.025 0.0 0.0 0.0 94-95 1.65 0.025 0.0 0.0 0.0 96-97 1.8375 0.025 0.0 0.0 0.0 98-99 1.975 0.025 0.0 0.0 0.0 100-101 2.2249999999999996 0.025 0.0 0.0 0.0 102-103 2.6624999999999996 0.025 0.0 0.0 0.0 104-105 2.8625 0.025 0.0 0.0 0.0 106-107 3.2125 0.025 0.0 0.0 0.0 108-109 3.5875 0.025 0.0 0.0 0.0 110-111 3.8125 0.025 0.0 0.0 0.0 112-113 4.375 0.025 0.0 0.0 0.0 114-115 5.125 0.025 0.0 0.0 0.0 116-117 5.875 0.025 0.0 0.0 0.0 118-119 6.425 0.025 0.0 0.0 0.0 120-121 7.0 0.025 0.0 0.0 0.0 122-123 7.825 0.025 0.0 0.0 0.0 124-125 8.375 0.025 0.0 0.0 0.0 126-127 9.5125 0.025 0.0 0.0 0.0 128-129 10.3875 0.025 0.0 0.0 0.0 130-131 11.075 0.025 0.0 0.0 0.0 132-133 11.9375 0.025 0.0 0.0 0.0 134-135 12.45 0.025 0.0 0.0 0.0 136-137 13.3125 0.025 0.0 0.0 0.0 138-139 14.1125 0.025 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GCAGCTT 10 0.006830828 145.0 2 CAGCTTT 10 0.006830828 145.0 3 >>END_MODULE SRR26075376 read2 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR26075376_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.06975 37.0 37.0 37.0 37.0 37.0 2 36.2095 37.0 37.0 37.0 37.0 37.0 3 36.222 37.0 37.0 37.0 37.0 37.0 4 36.228 37.0 37.0 37.0 37.0 37.0 5 36.2665 37.0 37.0 37.0 37.0 37.0 6 36.0745 37.0 37.0 37.0 37.0 37.0 7 36.135 37.0 37.0 37.0 37.0 37.0 8 36.075 37.0 37.0 37.0 37.0 37.0 9 36.1835 37.0 37.0 37.0 37.0 37.0 10-14 36.0418 37.0 37.0 37.0 37.0 37.0 15-19 36.013099999999994 37.0 37.0 37.0 37.0 37.0 20-24 35.9444 37.0 37.0 37.0 37.0 37.0 25-29 35.7883 37.0 37.0 37.0 37.0 37.0 30-34 35.61600000000001 37.0 37.0 37.0 37.0 37.0 35-39 35.5407 37.0 37.0 37.0 37.0 37.0 40-44 35.5004 37.0 37.0 37.0 37.0 37.0 45-49 35.4597 37.0 37.0 37.0 37.0 37.0 50-54 35.3646 37.0 37.0 37.0 37.0 37.0 55-59 35.3408 37.0 37.0 37.0 37.0 37.0 60-64 35.428999999999995 37.0 37.0 37.0 37.0 37.0 65-69 35.3343 37.0 37.0 37.0 37.0 37.0 70-74 35.2583 37.0 37.0 37.0 37.0 37.0 75-79 35.1453 37.0 37.0 37.0 34.6 37.0 80-84 35.2633 37.0 37.0 37.0 37.0 37.0 85-89 35.1914 37.0 37.0 37.0 37.0 37.0 90-94 35.215999999999994 37.0 37.0 37.0 37.0 37.0 95-99 35.3506 37.0 37.0 37.0 37.0 37.0 100-104 35.2721 37.0 37.0 37.0 34.6 37.0 105-109 35.2548 37.0 37.0 37.0 34.6 37.0 110-114 35.1807 37.0 37.0 37.0 27.4 37.0 115-119 35.2468 37.0 37.0 37.0 34.6 37.0 120-124 35.100899999999996 37.0 37.0 37.0 29.8 37.0 125-129 35.05309999999999 37.0 37.0 37.0 27.4 37.0 130-134 35.1493 37.0 37.0 37.0 32.2 37.0 135-139 34.914449999999995 37.0 37.0 37.0 25.0 37.0 140-144 34.96645 37.0 37.0 37.0 25.0 37.0 145-149 34.8728 37.0 37.0 37.0 25.0 37.0 150-151 34.66825 37.0 37.0 37.0 25.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.0 1101 2 0.0 1101 3 0.0 1101 4 0.0 1101 5 0.0 1101 6 0.0 1101 7 0.0 1101 8 0.0 1101 9 0.0 1101 10-14 0.0 1101 15-19 0.0 1101 20-24 0.0 1101 25-29 0.0 1101 30-34 0.0 1101 35-39 0.0 1101 40-44 0.0 1101 45-49 0.0 1101 50-54 0.0 1101 55-59 0.0 1101 60-64 0.0 1101 65-69 0.0 1101 70-74 0.0 1101 75-79 0.0 1101 80-84 0.0 1101 85-89 0.0 1101 90-94 0.0 1101 95-99 0.0 1101 100-104 0.0 1101 105-109 0.0 1101 110-114 0.0 1101 115-119 0.0 1101 120-124 0.0 1101 125-129 0.0 1101 130-134 0.0 1101 135-139 0.0 1101 140-144 0.0 1101 145-149 0.0 1101 150-151 0.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 12 1.0 13 3.0 14 9.0 15 10.0 16 4.0 17 7.0 18 2.0 19 7.0 20 4.0 21 18.0 22 14.0 23 15.0 24 23.0 25 22.0 26 34.0 27 22.0 28 13.0 29 18.0 30 22.0 31 32.0 32 48.0 33 93.0 34 206.0 35 665.0 36 2517.0 37 191.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.51112778194549 25.481370342585645 8.777194298574644 21.230307576894223 2 31.6 25.3 25.8 17.299999999999997 3 25.8 28.575 27.275 18.35 4 27.85 33.550000000000004 21.475 17.125 5 28.775000000000002 35.15 19.25 16.825000000000003 6 26.35 39.324999999999996 18.8 15.525 7 25.3 22.0 33.650000000000006 19.05 8 24.15 26.224999999999998 28.125 21.5 9 26.700000000000003 25.124999999999996 27.250000000000004 20.925 10-14 26.695 29.154999999999998 23.925 20.225 15-19 26.72 27.405 25.56 20.315 20-24 26.505000000000003 28.58 24.965 19.950000000000003 25-29 27.715 27.46 25.080000000000002 19.744999999999997 30-34 26.290000000000003 28.505000000000003 24.610000000000003 20.595 35-39 26.55 27.48 25.790000000000003 20.18 40-44 27.034999999999997 28.265 25.005 19.695 45-49 26.13 28.33 26.205000000000002 19.335 50-54 24.349999999999998 28.505000000000003 27.125 20.02 55-59 26.32 27.685 25.525 20.47 60-64 26.700000000000003 27.725 25.27 20.305 65-69 26.974999999999998 27.935 25.47 19.62 70-74 25.55 28.73 25.919999999999998 19.8 75-79 24.855 27.915 26.76 20.47 80-84 26.715 27.310000000000002 25.585 20.39 85-89 26.08 27.51 26.26 20.150000000000002 90-94 26.815 27.87 25.89 19.425 95-99 26.169999999999998 27.810000000000002 26.745 19.275000000000002 100-104 26.674999999999997 28.07 25.765 19.49 105-109 26.88 27.229999999999997 26.705000000000002 19.185 110-114 26.355 27.785 25.924999999999997 19.935 115-119 26.825 28.084999999999997 26.669999999999998 18.42 120-124 27.355 27.6 26.41 18.634999999999998 125-129 26.884999999999998 27.245 27.11 18.759999999999998 130-134 28.084999999999997 27.224999999999998 26.529999999999998 18.16 135-139 28.516425821291065 26.151307565378268 26.561328066403323 18.770938546927347 140-144 28.71430714607191 27.56913537030555 25.41381207181077 18.30274541181177 145-149 28.351340536214487 27.81112444977991 25.325130052020807 18.512404961984792 150-151 28.95723930982746 26.71917979494874 25.743935983995996 18.579644911227806 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.5 5 1.5 6 3.0 7 2.5 8 1.0 9 1.5 10 2.0 11 1.5 12 1.0 13 0.5 14 0.0 15 0.0 16 0.5 17 1.0 18 1.0 19 1.0 20 1.0 21 0.5 22 3.0 23 4.0 24 1.5 25 3.5 26 4.5 27 3.0 28 2.5 29 4.0 30 6.5 31 7.5 32 8.5 33 17.0 34 23.5 35 37.5 36 56.0 37 65.0 38 90.5 39 139.0 40 164.0 41 194.0 42 232.5 43 244.0 44 268.5 45 275.5 46 262.5 47 246.5 48 235.0 49 250.5 50 216.5 51 163.5 52 144.5 53 112.0 54 93.0 55 70.0 56 52.5 57 49.0 58 30.5 59 16.5 60 16.0 61 15.5 62 9.0 63 6.5 64 5.0 65 4.0 66 2.5 67 1.0 68 3.5 69 4.0 70 1.0 71 1.5 72 2.5 73 1.5 74 3.0 75 4.5 76 5.0 77 4.5 78 4.0 79 5.0 80 5.5 81 5.5 82 3.0 83 1.5 84 5.0 85 6.0 86 5.0 87 5.0 88 7.5 89 6.5 90 1.0 91 1.0 92 1.5 93 0.5 94 0.5 95 1.0 96 2.0 97 2.0 98 1.0 99 1.0 100 10.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.025 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.005 140-144 0.015 145-149 0.04 150-151 0.025 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 64.275 #Duplication Level Percentage of deduplicated Percentage of total 1 68.37806301050176 43.95 2 18.51419681057954 23.799999999999997 3 7.584597432905485 14.625 4 3.072734344612991 7.9 5 1.5558148580318942 5.0 6 0.5056398288603656 1.95 7 0.2333722287047841 1.05 8 0.0 0.0 9 0.038895371450797356 0.22499999999999998 >10 0.11668611435239205 1.5 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG 32 0.8 No Hit GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGT 18 0.44999999999999996 No Hit GGTTCTTACTGTCACTGTGCCTAAGGAGGAAGTCAAGAAACCTGAAGTCA 10 0.25 No Hit CCTCTATGCATCAATCATTTCCATTTTTTCCTCCTCCATTTACCCCAACT 9 0.22499999999999998 No Hit GCTTTATGTCAGAGGCACAATCTTGGGTTACAAGAGGTCGAAGTCCAACC 7 0.17500000000000002 No Hit TGTGTGCCAAATGTAAAGGGAAAGGCTCAAAGAGTGGAGCCTCTGGGACA 7 0.17500000000000002 No Hit CTGGAAGTCGTCAATCTATTATTTGTGAAATGGACCCAGGTTTCTTCAGG 7 0.17500000000000002 No Hit CTGATGCTGAGTTCTATGATTGTTGAGTATGCAGAGAACTGTTAATGCAG 7 0.17500000000000002 No Hit GCTATGGCAACACTAGCTCTGGTGGTTCCGGTGCCGGACCTAAGATTGAG 7 0.17500000000000002 No Hit GCCGGGAAGCAGCTTGAAGATGGAAGGACCTTGGCTGATTACAATATCCA 7 0.17500000000000002 No Hit GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGT 6 0.15 No Hit GAGAGGATGAAGACAGAAGAAAATAAAGAAAGACAGAGAGAGAGAGAGAG 6 0.15 No Hit TGCGTCTTTTCCGAGACACCTCAAGGGAAGGTCGCCATGGAAGGGAAAGT 6 0.15 No Hit ATTCGAAGGTGTTCTATTTGAAGATGAAGGGAGATTATCACAGGTATTTG 6 0.15 No Hit ACATCCTGACACTCTTGTCAATATCTTAGTTGTCGCTTGAGGTACTTCCC 6 0.15 No Hit GAAAAGTTGAGCATTAAGAGGTGTGGAAAGTTGGAAAGCATTCCGAGATG 6 0.15 No Hit GCTCAATCTTGGTCGATGCAATCTTTCATGCTATTCCAGTGCCACCGGAA 6 0.15 No Hit GTACTCACAACTTCTGCTGGCATTATGGACCATGAGGAGGCTAGAAGGAA 6 0.15 No Hit GAAGAAGAAAGTATGGGCAAAACGAACTCCACCTTCCAGTGCAAGGCCAT 6 0.15 No Hit GTTCTCTCCAAGTGAAGCGCCCAAGGAAGTTTTCTGGCTTCCCATCACCA 6 0.15 No Hit CGGCGACTGAAGGACCCAGAATTGTGTGGAACGAGAAGCAGCGCAGGTTT 6 0.15 No Hit CTCTCAGCAGAGAAACCTTAGCATCTTGCCTCTGCAAAAATGGGGATTTC 6 0.15 No Hit TGTAAATTGACACTACCCAAACACGACGTGGCATGTTACGATTCGCGTTT 6 0.15 No Hit AATTCATAAAAGACTATGCTCATGTCGCCGATGTAATCGAACCCGTGAAG 5 0.125 No Hit GCACTCAAGGACATTGTTTGGAGCTGGCAAGGAGAACAGCAAAGGGAACA 5 0.125 No Hit TACTCATGCTTTTGAAACTACCTTTGATGATCTGGAGGGCTTGCAGGAGT 5 0.125 No Hit ATTGGGAAAGCCAAGATTGAGAGAGAAGGGAAGGATGTGACCATTACAGC 5 0.125 No Hit GAGAGAGAGATTGAAGCCGAGTCAGTTTATTTATCTTGTAGAACATGTTG 5 0.125 No Hit GCAGCTCGAGGATGGCCGCACCCTTGCTGATTACAACATCCAGAAGGAGT 5 0.125 No Hit CTAGAAGTGAAGAGAAAAACAGATGGAGAGTGGCTTTGGGTCAAGCCAAC 5 0.125 No Hit ATCGGGATAGCATAGATGCAGCACGGATAGTTCCAGCTTGGTTGAGCTGC 5 0.125 No Hit ATCAAACAACAACAAAGAGCTGTCGGAAGAAAGAAATTAGAAGATGCACA 5 0.125 No Hit AGAGTAGCAAGAATATGATTCCCTGTTCAAGTCCTTGTAGGTACGTGAAT 5 0.125 No Hit TGCAGTGAGCAAAATCCAGGATGGGCTCCAGTACTTGTCATATGTAGTCA 5 0.125 No Hit ACACAAGTAGCACCGGCAGGGACCATTGCTAAATTGAGGAATGCATATTG 5 0.125 No Hit GCCAGAGAGAAGCTCCTTGATGCTATCGATGATCTGAAGCTCGACTCATT 5 0.125 No Hit AGTCTTCTGATTCCGACTCAGGTTCGGAATCTGATTCTGATGACAAGAGC 5 0.125 No Hit ATGGAGCAGTATATTGCCCTTGTATTGGAGAGAGCACCAGGGTGGATGGA 5 0.125 No Hit GGAGGGTATGGCAATGGTTTCGCACCAGCTTATCAGCAGGCTTACTACCG 5 0.125 No Hit ATCGAGCTTATTGCTTCTCTTAGAAAATCTCTTGGGCAGAAAGAAACACA 5 0.125 No Hit CGAGTTGTTGAGCAAAATCCTGGCTGCCATCCCTTCTGCCGAACCACCTG 5 0.125 No Hit ATGACAAGGAAAGTTTGCGAGCCACTGGAAGAGGACTCTTTCCGGCCAGT 5 0.125 No Hit GTTCACGATGGTAATGGTCACGAATGGATCTTCTCTTGCACCATCAAGAG 5 0.125 No Hit CAGTTTCTCGCACCCTTCTCCAGTAAACCCTCAGCACCATAATTTTCATC 5 0.125 No Hit CGATGGCACGAGGAGCTGGAGCCACAGAATTCCTTGTTGGACATTTTGTC 5 0.125 No Hit GTTACATTCTTGAGAGGATCACAGAAATTGCTGGAGTGGTCGTTTCCTTT 5 0.125 No Hit CTCTCCCTGTCGGAGGAGGAATTATTACTATGGAAACGGGAACGGAAACT 5 0.125 No Hit ATCAGGTGGTAGAAAATGGATTGCAGATGGTTCATTGATAAACAGGAAAC 5 0.125 No Hit TATCAAACAAACATGGTGAAAAACTGATAGGCTTATTACATGACACTGGA 5 0.125 No Hit GAAGAAATTCCGATCGGAGGAAGGTAAACTGGTATTTGGAGATTTCAATT 5 0.125 No Hit GGCATTGCAGGCGATCCATTCAAGGAAATTTGCATCTCTAATGTTACAAT 5 0.125 No Hit CTTGGCTCGTAAGTATGCTTTTGGGAAGACGCTGGTGATTGGCTCAGGAC 5 0.125 No Hit GTTCAAGAGGCTCTAGCTCTCAATGAATCTGAACTACATGGCCGTCAATT 5 0.125 No Hit CACCAAAGTCTGAGAAGAAGGATTACGACGAGACTGATCTGGCCAACATT 5 0.125 No Hit ATTTCCGACGGAGTTTTTCCCCGTCCTATTTGCAATCCCTCGAATGGCTG 5 0.125 No Hit GCCCAAGGAAGTTTTCTGGCTTCCCATCACCAAATCCTGGTATAATGCTG 5 0.125 No Hit GGATTGATGTTGGAATCTCTCTCAGATTTAGAAATGGAGTGGAAAATGGT 5 0.125 No Hit ATGACAGTCTCGTTCTTTCCCCCTCAAGGGTTGTGCTGTGCACACCAAAG 5 0.125 No Hit GGAAAATGGAAGATGGGGCGGTGGCCAAAGAAGATTCTGAGGCCTACTCT 5 0.125 No Hit CTTACACTGGGGCACTAATCTGGGTATTCACGACCTCAATTTCGGATATA 5 0.125 No Hit GCCAAAGAAGATTCTGAGGCCTACTCTGCATGGGCTCCTGATCCAGTTAC 5 0.125 No Hit CCGGAGAAGAGAGTGACACGGAGTATCATGATAGTAAGACCACCGGGGTA 5 0.125 No Hit GGTCAAGGCTTCTATGGAGAATGGGGTGCTTACAGTGACGGTGCCGAAGG 5 0.125 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.025 0.0 0.0 0.0 0.0 52-53 0.025 0.0 0.0 0.0 0.0 54-55 0.025 0.0 0.0 0.0 0.0 56-57 0.05 0.0 0.0 0.0 0.0 58-59 0.05 0.0 0.0 0.0 0.0 60-61 0.07500000000000001 0.0 0.0 0.0 0.0 62-63 0.175 0.0 0.0 0.0 0.0 64-65 0.175 0.0 0.0 0.0 0.0 66-67 0.175 0.0 0.0 0.0 0.0 68-69 0.175 0.0 0.0 0.0 0.0 70-71 0.175 0.0 0.0 0.0 0.0 72-73 0.175 0.0 0.0 0.0 0.0 74-75 0.21250000000000002 0.0 0.0 0.0 0.0 76-77 0.2375 0.0 0.0 0.0 0.0 78-79 0.25 0.0 0.0 0.0 0.0 80-81 0.35 0.0 0.0 0.0 0.0 82-83 0.375 0.0 0.0 0.0 0.0 84-85 0.475 0.0 0.0 0.0 0.0 86-87 0.625 0.0 0.0 0.0 0.0 88-89 0.8999999999999999 0.0 0.0 0.0 0.0 90-91 1.025 0.0 0.0 0.0 0.0 92-93 1.2125 0.0 0.0 0.0 0.0 94-95 1.55 0.0 0.0 0.0 0.0 96-97 1.7625 0.0 0.0 0.0 0.0 98-99 1.9375 0.0 0.0 0.0 0.0 100-101 2.2 0.0 0.0 0.0 0.0 102-103 2.6375 0.0 0.0 0.0 0.0 104-105 2.875 0.0 0.0 0.0 0.0 106-107 3.2375 0.0 0.0 0.0 0.0 108-109 3.6500000000000004 0.0 0.0 0.0 0.0 110-111 3.9125 0.0 0.0 0.0 0.0 112-113 4.475 0.0 0.0 0.0 0.0 114-115 5.25 0.0 0.0 0.0 0.0 116-117 6.025 0.0 0.0 0.0 0.0 118-119 6.575 0.0 0.0 0.0 0.0 120-121 7.2 0.0 0.0 0.0 0.0 122-123 8.0125 0.0 0.0 0.0 0.0 124-125 8.5625 0.0 0.0 0.0 0.0 126-127 9.6625 0.0 0.0 0.0 0.0 128-129 10.5375 0.0 0.0 0.0 0.0 130-131 11.25 0.0 0.0 0.0 0.0 132-133 12.149999999999999 0.0 0.0 0.0 0.0 134-135 12.7375 0.0 0.0 0.0 0.0 136-137 13.6875 0.0 0.0 0.0 0.0 138-139 14.4875 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content pass >>END_MODULE Read 4059787 spots for SRR26075376.sra Written 4059787 spots for SRR26075376.sra Read 4059787 spots for SRR26075376.sra Written 4059787 spots for SRR26075376.sra Read 4059787 spots for SRR26075376.sra Written 4059787 spots for SRR26075376.sra Read 4059787 spots for SRR26075376.sra Written 4059787 spots for SRR26075376.sra Read 4059787 spots for SRR26075376.sra Written 4059787 spots for SRR26075376.sra Read 4059787 spots for SRR26075376.sra Written 4059787 spots for SRR26075376.sra Read 4059787 spots for SRR26075376.sra Written 4059787 spots for SRR26075376.sra Read 4059787 spots for SRR26075376.sra Written 4059787 spots for SRR26075376.sra Read 4059787 spots for SRR26075376.sra Written 4059787 spots for SRR26075376.sra Read 4059787 spots for SRR26075376.sra Written 4059787 spots for SRR26075376.sra Read 4059787 spots for SRR26075376.sra Written 4059787 spots for SRR26075376.sra Read 4059787 spots for SRR26075376.sra Written 4059787 spots for SRR26075376.sra Read 4059787 spots for SRR26075376.sra Written 4059787 spots for SRR26075376.sra Read 4059794 spots for SRR26075376.sra Written 4059794 spots for SRR26075376.sra Read 4059787 spots for SRR26075376.sra Written 4059787 spots for SRR26075376.sra Read 4059787 spots for SRR26075376.sra Written 4059787 spots for SRR26075376.sra Read 4059787 spots for SRR26075376.sra Written 4059787 spots for SRR26075376.sra Read 4059787 spots for SRR26075376.sra Written 4059787 spots for SRR26075376.sra Read 4059787 spots for SRR26075376.sra Written 4059787 spots for SRR26075376.sra Read 4059787 spots for SRR26075376.sra Written 4059787 spots for SRR26075376.sra SRR ids: ['SRR26075376.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_1_1wqds9 SRR26075376.sra spots: 81195747 blocks: [[1, 4059787], [4059788, 8119574], [8119575, 12179361], [12179362, 16239148], [16239149, 20298935], [20298936, 24358722], [24358723, 28418509], [28418510, 32478296], [32478297, 36538083], [36538084, 40597870], [40597871, 44657657], [44657658, 48717444], [48717445, 52777231], [52777232, 56837018], [56837019, 60896805], [60896806, 64956592], [64956593, 69016379], [69016380, 73076166], [73076167, 77135953], [77135954, 81195747]] SRR26075376 file size 29998708 SRR26075376 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o SRR26075376 SRR26075376_1.fastq SRR26075376_2.fastq Input file: SRR26075376_1.fastq Paired file: SRR26075376_2.fastq trimmed: SRR26075376-trimmed-pair1.fastq, SRR26075376-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Tue Feb 11 23:45:09 2025 >> started Tue Feb 11 23:46:53 2025 >> done (104.843s) 81195747 read pairs processed; of these: 985 ( 0.00%) short read pairs filtered out after trimming by size control 1102729 ( 1.36%) empty read pairs filtered out after trimming by size control 80092033 (98.64%) read pairs available; of these: 16429319 (20.51%) trimmed read pairs available after processing 63662714 (79.49%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 18 86 0.00% 19 87 0.00% 20 132 0.00% 21 139 0.00% 22 180 0.00% 23 152 0.00% 24 198 0.00% 25 261 0.00% 26 255 0.00% 27 275 0.00% 28 258 0.00% 29 271 0.00% 30 255 0.00% 31 281 0.00% 32 343 0.00% 33 353 0.00% 34 357 0.00% 35 357 0.00% 36 369 0.00% 37 410 0.00% 38 451 0.00% 39 510 0.00% 40 635 0.00% 41 566 0.00% 42 634 0.00% 43 671 0.00% 44 671 0.00% 45 726 0.00% 46 919 0.00% 47 905 0.00% 48 994 0.00% 49 1168 0.00% 50 1242 0.00% 51 1335 0.00% 52 1478 0.00% 53 1558 0.00% 54 1726 0.00% 55 1844 0.00% 56 1910 0.00% 57 2196 0.00% 58 2436 0.00% 59 2646 0.00% 60 3091 0.00% 61 3349 0.00% 62 3857 0.00% 63 4543 0.01% 64 4833 0.01% 65 4865 0.01% 66 5706 0.01% 67 6346 0.01% 68 7156 0.01% 69 7826 0.01% 70 8859 0.01% 71 10091 0.01% 72 12054 0.02% 73 13472 0.02% 74 15212 0.02% 75 16314 0.02% 76 18434 0.02% 77 20344 0.03% 78 22810 0.03% 79 24804 0.03% 80 27904 0.03% 81 31796 0.04% 82 35187 0.04% 83 39827 0.05% 84 44636 0.06% 85 48859 0.06% 86 52615 0.07% 87 56137 0.07% 88 60097 0.08% 89 65204 0.08% 90 70361 0.09% 91 77405 0.10% 92 82564 0.10% 93 89985 0.11% 94 96225 0.12% 95 101838 0.13% 96 109641 0.14% 97 115192 0.14% 98 119018 0.15% 99 125430 0.16% 100 132168 0.17% 101 137089 0.17% 102 145616 0.18% 103 153164 0.19% 104 161014 0.20% 105 169100 0.21% 106 177607 0.22% 107 185093 0.23% 108 187439 0.23% 109 194521 0.24% 110 194935 0.24% 111 203994 0.25% 112 211258 0.26% 113 219584 0.27% 114 229258 0.29% 115 236462 0.30% 116 241494 0.30% 117 247780 0.31% 118 256797 0.32% 119 258898 0.32% 120 265665 0.33% 121 268504 0.34% 122 273674 0.34% 123 278255 0.35% 124 287344 0.36% 125 295484 0.37% 126 304999 0.38% 127 310725 0.39% 128 314933 0.39% 129 316879 0.40% 130 325778 0.41% 131 330117 0.41% 132 333041 0.42% 133 343641 0.43% 134 346697 0.43% 135 352738 0.44% 136 359127 0.45% 137 362250 0.45% 138 368475 0.46% 139 376035 0.47% 140 375859 0.47% 141 381050 0.48% 142 383502 0.48% 143 388994 0.49% 144 393215 0.49% 145 401927 0.50% 146 402462 0.50% 147 408141 0.51% 148 408070 0.51% 149 412314 0.51% 150 420026 0.52% 151 63662714 79.49% 80092033 reads passed initial QC criterion=sequence-density sequence-density=0.74 sequence-density-rank=1 fanout-score=3.50 fanout-score-rank=23 prefix-density=0.81 prefix-fanout=3.2 sequence=GCATTCTCAGGCAGCCTAAA criterion=fanout-score sequence-density=0.22 sequence-density-rank=16 fanout-score=18.67 fanout-score-rank=1 prefix-density=0.17 prefix-fanout=18.7 sequence=GGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCTCAGTTATCTCGTATGCCGTCTTCTGCTTG criterion=sequence-density sequence-density=0.43 sequence-density-rank=1 fanout-score=4.52 fanout-score-rank=22 prefix-density=0.93 prefix-fanout=2.1 sequence=CCTGAGAATGCTAA criterion=fanout-score sequence-density=0.02 sequence-density-rank=42 fanout-score=296.94 fanout-score-rank=1 prefix-density=0.43 prefix-fanout=14.3 sequence=GAGAAGAAACACTAAAAAAATCACACAAGCGAAATCTCCAGCGAGAAGAGAAAGATGGATTTCAGAATCATGGGTCTTGACGCGCCACTCTTCAACACCCTCCAGCACATGATGGATGCAAGTGATCATGAGGCAGACAAGTCCTTCAATGCGCCAACACGCACTTACGTACGTGATGCCAAGGCAATGGCATCAACACCAGCTGATGTGAAAGAGTATCCAAACTCTTATGCGTTCGTCATTGACATGCCGGGACTGAAATCAGGGGACATCAAGGTTCAAGTGGAGGATGACAATGTGCTGGTTATCAGTGGAGAGAG SRR26075376 testing PE reads STAR mapping to Ensembl genome Started job on | Feb 11 23:49:17 Started mapping on | Feb 11 23:49:17 Finished on | Feb 12 00:01:37 Mapping speed, Million of reads per hour | 389.64 Number of input reads | 80092033 Average input read length | 283 UNIQUE READS: Uniquely mapped reads number | 60566296 Uniquely mapped reads % | 75.62% Average mapped length | 285.08 Number of splices: Total | 43662627 Number of splices: Annotated (sjdb) | 42306499 Number of splices: GT/AG | 42840543 Number of splices: GC/AG | 552974 Number of splices: AT/AC | 56572 Number of splices: Non-canonical | 212538 Mismatch rate per base, % | 0.52% Deletion rate per base | 0.04% Deletion average length | 2.96 Insertion rate per base | 0.04% Insertion average length | 2.97 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 2049543 % of reads mapped to multiple loci | 2.56% Number of reads mapped to too many loci | 243771 % of reads mapped to too many loci | 0.30% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 21.15% % of reads unmapped: other | 0.37% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 17476194 17476194 17476194 N_multimapping 2049543 2049543 2049543 N_noFeature 1831805 59813317 2268881 N_ambiguous 1766378 19969 1435030 UnstrandedReadsAssigned:56968113 PositiveStrandReadsAssigned:733010 NegativeStrandReadsAssigned:56862385 Dataset is classified negative stranded MeadianReadLen=151 20thPercentileLength=148 echo kmer=143 SRR26075376 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,400 [index] number of k-mers: 62,057,036 [index] number of equivalence classes: 130,681 [quant] running in paired-end mode [quant] will process pair 1: SRR26075376-trimmed-pair1.fastq SRR26075376-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 80,092,033 reads, 70,119,181 reads pseudoaligned [quant] estimated average fragment length: 191.287 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,309 rounds 52401 SRR26075376.ke.tsv 34699 SRR26075376.se.tsv 87100 total ==> SRR26075376.ke.tsv <== target_id length eff_length est_counts tpm Potri.005G200100.1.v4.1 2018 1827.71 11979 64.1449 Potri.005G024800.1.v4.1 1035 844.713 49205 570.098 Potri.004G059700.1.v4.1 961 770.729 89 1.13015 Potri.007G009000.2.v4.1 1416 1225.71 0 0 Potri.003G141000.2.v4.1 2943 2752.71 2663 9.46803 Potri.016G087400.1.v4.1 270 100.926 7691.04 745.818 Potri.015G069301.1.v4.1 564 374.282 0 0 Potri.010G195200.1.v4.1 1773 1582.71 428 2.64662 Potri.012G127500.1.v4.1 977 786.729 14769 183.728 ==> SRR26075376.se.tsv <== Potri.001G166300.v4.1 0 Potri.001G448400.v4.1 207 Potri.001G233950.v4.1 0 Potri.001G122700.v4.1 873 Potri.001G212900.v4.1 0 Potri.001G182400.v4.1 0 Potri.001G256600.v4.1 0 Potri.001G040500.v4.1 0 Potri.001G416900.v4.1 0 Potri.001G452600.v4.1 485 SRR26075376 completed mapping pipeline successfully