Starting /dee2/code/volunteer_pipeline.sh SRR26075377 current disk space = 3052341456896 free memory = 1432978284 SRR26075377 SRAfilesize 54f1a9a6f5f154f87ab41d6d91b8acc5 SRR26075377.sra SRR26075377.sra file validated SRR26075377 is paired end SRR26075377 is conventional basespace SRR26075377 read1 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR26075377_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.494 37.0 37.0 37.0 37.0 37.0 2 36.466 37.0 37.0 37.0 37.0 37.0 3 36.5365 37.0 37.0 37.0 37.0 37.0 4 36.5645 37.0 37.0 37.0 37.0 37.0 5 36.59 37.0 37.0 37.0 37.0 37.0 6 36.7005 37.0 37.0 37.0 37.0 37.0 7 36.531 37.0 37.0 37.0 37.0 37.0 8 36.6165 37.0 37.0 37.0 37.0 37.0 9 36.71 37.0 37.0 37.0 37.0 37.0 10-14 36.6005 37.0 37.0 37.0 37.0 37.0 15-19 36.5781 37.0 37.0 37.0 37.0 37.0 20-24 36.5363 37.0 37.0 37.0 37.0 37.0 25-29 36.4053 37.0 37.0 37.0 37.0 37.0 30-34 36.3668 37.0 37.0 37.0 37.0 37.0 35-39 36.373 37.0 37.0 37.0 37.0 37.0 40-44 36.2027 37.0 37.0 37.0 37.0 37.0 45-49 35.780100000000004 37.0 37.0 37.0 37.0 37.0 50-54 35.8803 37.0 37.0 37.0 37.0 37.0 55-59 35.4113 37.0 37.0 37.0 37.0 37.0 60-64 35.4219 37.0 37.0 37.0 37.0 37.0 65-69 35.303399999999996 37.0 37.0 37.0 37.0 37.0 70-74 35.7411 37.0 37.0 37.0 37.0 37.0 75-79 35.96 37.0 37.0 37.0 37.0 37.0 80-84 35.97840000000001 37.0 37.0 37.0 37.0 37.0 85-89 35.8543 37.0 37.0 37.0 37.0 37.0 90-94 35.8784 37.0 37.0 37.0 37.0 37.0 95-99 35.85 37.0 37.0 37.0 37.0 37.0 100-104 35.7816 37.0 37.0 37.0 37.0 37.0 105-109 35.67479999999999 37.0 37.0 37.0 37.0 37.0 110-114 35.5505 37.0 37.0 37.0 37.0 37.0 115-119 35.5191 37.0 37.0 37.0 37.0 37.0 120-124 35.5609 37.0 37.0 37.0 37.0 37.0 125-129 35.38099999999999 37.0 37.0 37.0 37.0 37.0 130-134 35.275099999999995 37.0 37.0 37.0 32.2 37.0 135-139 35.2005 37.0 37.0 37.0 29.8 37.0 140-144 35.1058 37.0 37.0 37.0 32.2 37.0 145-149 35.0917 37.0 37.0 37.0 27.4 37.0 150-151 35.05775 37.0 37.0 37.0 25.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.0 1101 2 0.0 1101 3 0.0 1101 4 0.0 1101 5 0.0 1101 6 0.0 1101 7 0.0 1101 8 0.0 1101 9 0.0 1101 10-14 0.0 1101 15-19 0.0 1101 20-24 0.0 1101 25-29 0.0 1101 30-34 0.0 1101 35-39 0.0 1101 40-44 0.0 1101 45-49 0.0 1101 50-54 0.0 1101 55-59 0.0 1101 60-64 0.0 1101 65-69 0.0 1101 70-74 0.0 1101 75-79 0.0 1101 80-84 0.0 1101 85-89 0.0 1101 90-94 0.0 1101 95-99 0.0 1101 100-104 0.0 1101 105-109 0.0 1101 110-114 0.0 1101 115-119 0.0 1101 120-124 0.0 1101 125-129 0.0 1101 130-134 0.0 1101 135-139 0.0 1101 140-144 0.0 1101 145-149 0.0 1101 150-151 0.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 16 1.0 17 0.0 18 1.0 19 0.0 20 3.0 21 4.0 22 3.0 23 6.0 24 9.0 25 8.0 26 12.0 27 10.0 28 18.0 29 18.0 30 33.0 31 50.0 32 62.0 33 207.0 34 199.0 35 454.0 36 2720.0 37 182.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 43.30155544405419 13.49724034119418 10.66231811339689 32.53888610135474 2 17.5 16.950000000000003 36.65 28.9 3 18.975 22.425 34.150000000000006 24.45 4 22.2 29.125 23.825 24.85 5 24.075 34.225 24.224999999999998 17.474999999999998 6 23.95 34.225 23.5 18.325 7 15.55 27.675 39.85 16.925 8 16.6 28.299999999999997 30.15 24.95 9 20.225 20.45 32.4 26.924999999999997 10-14 20.307030703070307 28.457845784578456 25.86758675867587 25.367536753675367 15-19 20.21 27.365000000000002 28.144999999999996 24.279999999999998 20-24 20.525 27.83 27.6 24.044999999999998 25-29 21.025 27.279999999999998 27.639999999999997 24.055 30-34 18.75 27.825 28.749999999999996 24.675 35-39 19.975 26.685 27.794999999999998 25.545 40-44 20.465 27.939999999999998 27.525 24.07 45-49 20.665 26.91 27.389999999999997 25.035 50-54 20.76 27.26 27.310000000000002 24.67 55-59 20.985 26.584999999999997 28.275 24.154999999999998 60-64 22.065 26.195 28.02 23.72 65-69 20.68 28.035 27.87 23.415 70-74 22.715 26.889999999999997 27.015 23.380000000000003 75-79 23.41 27.615000000000002 25.865 23.11 80-84 24.925 25.535000000000004 26.61 22.93 85-89 23.294999999999998 27.655 25.990000000000002 23.06 90-94 24.349999999999998 25.674999999999997 26.115 23.86 95-99 23.97 25.935000000000002 26.340000000000003 23.755000000000003 100-104 23.865 26.77 26.375 22.99 105-109 24.165 26.495 26.025 23.315 110-114 23.96 25.905 26.650000000000002 23.485 115-119 25.074999999999996 26.8 25.45 22.675 120-124 23.575 26.415 26.27 23.74 125-129 24.654999999999998 25.580000000000002 25.755 24.01 130-134 23.905 26.740000000000002 25.885 23.47 135-139 25.55 26.265 24.905 23.28 140-144 24.735 26.105 25.55 23.61 145-149 25.82 26.655 24.33 23.195 150-151 24.725 26.700000000000003 25.412499999999998 23.1625 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1.0 1 1.0 2 0.5 3 0.0 4 0.5 5 0.5 6 0.5 7 1.0 8 1.0 9 1.5 10 1.0 11 0.5 12 0.5 13 0.0 14 0.0 15 1.0 16 1.5 17 0.5 18 0.0 19 0.0 20 0.5 21 1.5 22 2.0 23 2.5 24 2.5 25 5.0 26 8.0 27 6.0 28 11.5 29 20.5 30 22.5 31 22.5 32 27.0 33 47.0 34 56.5 35 51.5 36 66.0 37 82.5 38 92.5 39 127.0 40 153.5 41 170.5 42 215.5 43 227.5 44 219.5 45 250.5 46 265.5 47 229.0 48 213.5 49 215.0 50 185.5 51 153.5 52 154.0 53 120.5 54 77.0 55 78.0 56 67.0 57 45.0 58 34.0 59 24.5 60 16.5 61 18.5 62 13.5 63 9.0 64 5.0 65 10.5 66 29.5 67 36.0 68 31.5 69 27.5 70 16.0 71 6.5 72 4.5 73 4.0 74 2.5 75 1.0 76 0.0 77 0.0 78 0.5 79 0.5 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.5 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.35000000000000003 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.01 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150-151 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 61.824999999999996 #Duplication Level Percentage of deduplicated Percentage of total 1 66.39708855640922 41.05 2 19.611807521229277 24.25 3 8.451273756570966 15.675 4 2.8305701577031948 7.000000000000001 5 1.4557217953902144 4.5 6 0.4852405984634048 1.7999999999999998 7 0.4043671653861707 1.7500000000000002 8 0.1213101496158512 0.6 9 0.1213101496158512 0.675 >10 0.08087343307723413 1.0999999999999999 >50 0.040436716538617065 1.6 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCTCACATCTCGTAT 64 1.6 TruSeq Adapter, Index 13 (97% over 37bp) GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCTCACATCGCGTAT 34 0.8500000000000001 TruSeq Adapter, Index 13 (97% over 37bp) GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCTCACATCTCGTTT 10 0.25 TruSeq Adapter, Index 13 (97% over 37bp) GTCTTTTTTCACTCTCATCACCGACACTTCACCGCCATGGCTACCACTAC 9 0.22499999999999998 No Hit GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCTCACATCGCGTTT 9 0.22499999999999998 TruSeq Adapter, Index 13 (97% over 37bp) AGAACAAGCAGACCTAACTCGTGAGCTCTATCTATCAGAGACTTCAGATC 9 0.22499999999999998 No Hit AAATGAACCAAGAAGACTGCAAATCCAATGGGAAGGGGAGCCAAAATAGG 8 0.2 No Hit CTTGACTGTCTCCTTCATCTTTTAGTGGGAAAGGCGAGTCAGTTATTCTC 8 0.2 No Hit GTAGGTGAACAAATTCTGATGACCCTTCAAATTTTTTGGAGGAATCATAG 8 0.2 No Hit GTATAAAGATAACTATTTCTCAGATCCAAACATTCACGAATCATCTTACG 7 0.17500000000000002 No Hit CCATTGTTTCGCAGCATCAACATTTGCAGGAGATTCGTCATTTGGACTAG 7 0.17500000000000002 No Hit CCAGGACACGATAATAAACAAAAGGGAAATGACTGACCAGCAAAACTTAA 7 0.17500000000000002 No Hit CTATGATCCTTGAATATTTTTATCAATTTAAGCCCTTGCTCTAACTTGAC 7 0.17500000000000002 No Hit GGAAGAATTGAATGTGGAGCTGTGACAGGAAATCCTCCAAGCAACTGTGG 7 0.17500000000000002 No Hit TCCTCAGCCACATCTTTTGGAGCCTCAGCTACAGGTTCAGGTTCAGGTTC 7 0.17500000000000002 No Hit CTCCAAGTCCCGGACCCCATCCTTGTCCCGGACCCCATCCTCCTGGTCCC 7 0.17500000000000002 No Hit GCCAGGCAGAATGGGTGGTCGAACATGATTTGGTGATCGATGATGTCCCA 7 0.17500000000000002 No Hit TTTTTTTTAAAATAATCATATGAAACTTAGGTATAGAACCAGTACCAACT 7 0.17500000000000002 No Hit CTTGATATGTACTGATCAAGTGATGTCAATTTACCATCCGACTTGGTGGT 7 0.17500000000000002 No Hit GTTCTCATCTCCTGTAAGCATGGAAGGATAGCAGGTCTACGCTGCTGTAA 6 0.15 No Hit CGAAGATCTGACGATCTTGTTCCCGCAATAACATTCTCGATGAGTGCAGC 6 0.15 No Hit ATGCTACTTATTTTATAAAATACCACAACCATTATAAAATTCATCAGCCT 6 0.15 No Hit CTTGTTGATTATTCATAGTAGTAGTTCGTAAATTTGCTATAATAGCATCA 6 0.15 No Hit ATATCATTCGCCGTAAGGAGAAGTAGGAATTCTGGGTCCAACCTCGGTTG 6 0.15 No Hit CACAGGTCCAAGCTGGACTAGGAACAGAGATGAATTATCATTTTCTGACG 6 0.15 No Hit GGGAAGTGAACAGAATTGGTCCCAGGAAGCTAAAGTCCTTGCCCTTCTTT 6 0.15 No Hit CCTCTCAATAAATCTTCTTTTAATCCCATCTCATCGAAACTTGCCACAGG 6 0.15 No Hit GTTGTTTACTGGGTCAGAAAGGTGGTCAGCCAGGTTCTCCAGTGGTCCCT 6 0.15 No Hit GCCAAATCCCTTCACGGGTTCGATTACATCGGCGACATGAGCATAGTCTT 6 0.15 No Hit GGAAGCTCCTGACTTGACGGGGTGGATCAGCCTCTTCCTCCCGGAGCTTC 6 0.15 No Hit CTCAGTTTCCATGTCAATGCAAAGCAGCCCACCAAACCCATATTCTTTAT 6 0.15 No Hit TTTGGAACACTTTCAAACATATCTCCTCCCACGTGTTCCACACCAGGACA 5 0.125 No Hit CCCACCTCTGTTCCTTCGGCTGGCTGTTTGGTAGTATAATCTGCCCCAGA 5 0.125 No Hit ATTGCAACTTTCTGAGGCATTTTCTTGTTTGGGCTCTTTGTTTCTTTATC 5 0.125 No Hit GGGATGGAGGCGGTAGCAGTGTGGGAGAGGTTGTCCATGGAGGCGATGGA 5 0.125 No Hit GTCAAAGCACCTCCAAGCATGGCATTCTTCCAGTCATGAGTGCCACGAAT 5 0.125 No Hit ATGTACTTCATCTTCATCCTCAGCCGTGTCAGCTTTGGTCTCGGCATCGG 5 0.125 No Hit GAACCAACCAAAAATCAGGCATCCTACCATTGGTGATCAATCAAAGTTCA 5 0.125 No Hit TATAAAGGACTCGACCATTCCCTTCCTCTCCTCCTTTGAGCGCCGAATCC 5 0.125 No Hit CCTCAATGTTTTTCTTAAAATGTTCTTTGTTTTCGTCATCGAGTTTCTCC 5 0.125 No Hit TAACAGGAAAAGCAGAATCCGTAATCTCCACGCCGCAAATATCACAATCT 5 0.125 No Hit CTGGTTTGTTCTCGTTGTTCTCGAGGGTAATATGCTTCAGGAAACTGTTG 5 0.125 No Hit CAGATTTTTCCTAATTGAGTAAACAGCAAACCAACCATCCAAACGACATG 5 0.125 No Hit GTGGGATGTCACAGACACTGGATTTCACATTGTTTGGAATCCACTCAACA 5 0.125 No Hit GATTAGCTTAGCATACTCTTCATCCATTTTGCTTTCAAAATCCCCCCCAA 5 0.125 No Hit CCAAGGCTCACTTTCAAAATCTTCATCATCATTGCTATTGTCACCATCGT 5 0.125 No Hit GACTACAATTGAGTCTGCAATTTGCACACCTCCACCTCCAAATCCCAATT 5 0.125 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5 0.125 No Hit CCAAGAGGTTCACATCCCCAGAAGACAAGATTAAGGACACCACCTTCTAT 5 0.125 No Hit GATGACAATTGTCTGAGATTAATTTATGTCAGCCACCACAAATCACCAAA 5 0.125 No Hit GTCCTGCACCACTGTACCATTACAGCAGAACTCTTTCTTGAGGTCCTTTA 5 0.125 No Hit GCCACAATCGCTAGCCACCTTTCTAGCACCAGGAGAGGAAACAAACTGCT 5 0.125 No Hit CCTAGAAGAGCCTTCTGGGGCCTTGCGGTACTTAGTCCAGAAGTGGACGA 5 0.125 No Hit GGAGTGGAGGGATATTTGACCTAAAGGAAGGACCGAAAGAAGACCCTATC 5 0.125 No Hit GGCTGACTCTCACCATACGACAACTTGGCAAAGATTGACTGATAAGGTGG 5 0.125 No Hit GTGTCGTCGGTGTCCCTGGAGTTCCTGGCATAGTCATGGACCTCTGAAAC 5 0.125 No Hit GCGTCAGACTCAATCAATGCAAGGATAGTGCCATATTCAAATGATGAAAG 5 0.125 No Hit GAGGGCGATCGACAAAGCTGCGCAAACTCTGCCAAATCAATGTGTCCGTC 5 0.125 No Hit GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCTCACATCTCGGAT 5 0.125 TruSeq Adapter, Index 13 (97% over 37bp) CTAGATTTGTCTGAAAACTCGCCTGGGTGGGGGTGACATTGGAGCATGTT 5 0.125 No Hit CTCATACTCTACAGTCCCCAACAGGTTCTCTCCATTCTCTGGATAATTGA 5 0.125 No Hit CCCCTCACTATACACCCTTGAGAGACAAGTATCATCCGATGCTCCTCAGG 5 0.125 No Hit CTCAGAGCAGACTGGGCACCAGGCCCAGGTGTCTTAGTTTTGTTCCCTCC 5 0.125 No Hit CCCAAGAACAATGTCACTCACTTTAGATTGCGCATCCTTGAAAATTTGCA 5 0.125 No Hit ATTGGTCCCAATCACACCTGAGAAGTTAACAATCGGGTTTACTGTCAACC 5 0.125 No Hit ATAAGCTCCATATGGTGCCAAAGCCAAGTCTAAGCCATTGTAGGAAAGTT 5 0.125 No Hit GGGCATTTCACATTGGAAAATGATGGTCAGCTTGGTGGCAGTTATTTTCT 5 0.125 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0125 0.0 0.0 0.0 0.0 26-27 0.025 0.0 0.0 0.0 0.0 28-29 0.025 0.0 0.0 0.0 0.0 30-31 0.025 0.0 0.0 0.0 0.0 32-33 0.025 0.0 0.0 0.0 0.0 34-35 0.025 0.0 0.0 0.0 0.0 36-37 0.025 0.0 0.0 0.0 0.0 38-39 0.025 0.0 0.0 0.0 0.0 40-41 0.025 0.0 0.0 0.0 0.0 42-43 0.025 0.0 0.0 0.0 0.0 44-45 0.025 0.0 0.0 0.0 0.0 46-47 0.025 0.0 0.0 0.0 0.0 48-49 0.037500000000000006 0.0 0.0 0.0 0.0 50-51 0.05 0.0 0.0 0.0 0.0 52-53 0.05 0.0 0.0 0.0 0.0 54-55 0.075 0.0 0.0 0.0 0.0 56-57 0.075 0.0 0.0 0.0 0.0 58-59 0.075 0.0 0.0 0.0 0.0 60-61 0.075 0.0 0.0 0.0 0.0 62-63 0.075 0.0 0.0 0.0 0.0 64-65 0.0875 0.0 0.0 0.0 0.0 66-67 0.1 0.0 0.0 0.0 0.0 68-69 0.1 0.0 0.0 0.0 0.0 70-71 0.1 0.0 0.0 0.0 0.0 72-73 0.15 0.0 0.0 0.0 0.0 74-75 0.175 0.0 0.0 0.0 0.0 76-77 0.21250000000000002 0.0 0.0 0.0 0.0 78-79 0.225 0.0 0.0 0.0 0.0 80-81 0.25 0.0 0.0 0.0 0.0 82-83 0.2875 0.0 0.0 0.0 0.0 84-85 0.4 0.0 0.0 0.0 0.0 86-87 0.5125 0.0 0.0 0.0 0.0 88-89 0.65 0.0 0.0 0.0 0.0 90-91 0.75 0.0 0.0 0.0 0.0 92-93 0.775 0.0 0.0 0.0 0.0 94-95 0.8 0.0 0.0 0.0 0.0 96-97 0.975 0.0 0.0 0.0 0.0 98-99 1.0875 0.0 0.0 0.0 0.0 100-101 1.225 0.0 0.0 0.0 0.0 102-103 1.4375 0.0 0.0 0.0 0.0 104-105 1.8125 0.0 0.0 0.0 0.0 106-107 1.9625 0.0 0.0 0.0 0.0 108-109 2.15 0.0 0.0 0.0 0.0 110-111 2.4625 0.0 0.0 0.0 0.0 112-113 2.6875 0.0 0.0 0.0 0.0 114-115 3.1125 0.0 0.0 0.0 0.0 116-117 3.55 0.0 0.0 0.0 0.0 118-119 4.075 0.0 0.0 0.0 0.0 120-121 4.5 0.0 0.0 0.0 0.0 122-123 4.875 0.0 0.0 0.0 0.0 124-125 5.3125 0.0 0.0 0.0 0.0 126-127 5.824999999999999 0.0 0.0 0.0 0.0 128-129 6.4375 0.0 0.0 0.0 0.0 130-131 7.0375 0.0 0.0 0.0 0.0 132-133 7.4375 0.0 0.0 0.0 0.0 134-135 8.2125 0.0 0.0 0.0 0.0 136-137 8.837499999999999 0.0 0.0 0.0 0.0 138-139 9.675 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTACAGT 10 0.006830828 145.0 9 ACTCTAC 10 0.006830828 145.0 6 TCTACAG 10 0.006830828 145.0 8 CTCTACA 10 0.006830828 145.0 7 AACTCAA 10 0.006830828 145.0 5 GTCCAAC 10 0.006830828 145.0 1 TTTACTT 10 0.006830828 145.0 2 >>END_MODULE SRR26075377 read2 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR26075377_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 35.759 37.0 37.0 37.0 37.0 37.0 2 36.0775 37.0 37.0 37.0 37.0 37.0 3 35.961 37.0 37.0 37.0 37.0 37.0 4 35.8935 37.0 37.0 37.0 37.0 37.0 5 35.877 37.0 37.0 37.0 37.0 37.0 6 35.6825 37.0 37.0 37.0 37.0 37.0 7 35.5985 37.0 37.0 37.0 37.0 37.0 8 35.592 37.0 37.0 37.0 37.0 37.0 9 35.4955 37.0 37.0 37.0 37.0 37.0 10-14 35.3524 37.0 37.0 37.0 37.0 37.0 15-19 35.0828 37.0 37.0 37.0 27.4 37.0 20-24 34.718900000000005 37.0 37.0 37.0 25.0 37.0 25-29 34.4142 37.0 37.0 37.0 25.0 37.0 30-34 34.0613 37.0 37.0 37.0 25.0 37.0 35-39 33.859500000000004 37.0 37.0 37.0 22.2 37.0 40-44 33.7738 37.0 37.0 37.0 16.6 37.0 45-49 33.5355 37.0 37.0 37.0 11.0 37.0 50-54 33.2932 37.0 37.0 37.0 11.0 37.0 55-59 33.403499999999994 37.0 37.0 37.0 11.0 37.0 60-64 33.58 37.0 37.0 37.0 11.0 37.0 65-69 33.399499999999996 37.0 37.0 37.0 11.0 37.0 70-74 33.3226 37.0 37.0 37.0 11.0 37.0 75-79 33.2173 37.0 37.0 37.0 11.0 37.0 80-84 33.2641 37.0 37.0 37.0 11.0 37.0 85-89 33.2923 37.0 37.0 37.0 11.0 37.0 90-94 33.3817 37.0 37.0 37.0 11.0 37.0 95-99 33.5918 37.0 37.0 37.0 13.8 37.0 100-104 33.66180000000001 37.0 37.0 37.0 13.8 37.0 105-109 33.679 37.0 37.0 37.0 16.6 37.0 110-114 33.6635 37.0 37.0 37.0 16.6 37.0 115-119 33.4989 37.0 37.0 37.0 11.0 37.0 120-124 33.543 37.0 37.0 37.0 13.8 37.0 125-129 33.620999999999995 37.0 37.0 37.0 13.8 37.0 130-134 33.5804 37.0 37.0 37.0 13.8 37.0 135-139 33.3582 37.0 37.0 37.0 11.0 37.0 140-144 33.4003 37.0 37.0 37.0 11.0 37.0 145-149 33.362700000000004 37.0 37.0 37.0 11.0 37.0 150-151 33.22775 37.0 37.0 37.0 11.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.0 1101 2 0.0 1101 3 0.0 1101 4 0.0 1101 5 0.0 1101 6 0.0 1101 7 0.0 1101 8 0.0 1101 9 0.0 1101 10-14 0.0 1101 15-19 0.0 1101 20-24 0.0 1101 25-29 0.0 1101 30-34 0.0 1101 35-39 0.0 1101 40-44 0.0 1101 45-49 0.0 1101 50-54 0.0 1101 55-59 0.0 1101 60-64 0.0 1101 65-69 0.0 1101 70-74 0.0 1101 75-79 0.0 1101 80-84 0.0 1101 85-89 0.0 1101 90-94 0.0 1101 95-99 0.0 1101 100-104 0.0 1101 105-109 0.0 1101 110-114 0.0 1101 115-119 0.0 1101 120-124 0.0 1101 125-129 0.0 1101 130-134 0.0 1101 135-139 0.0 1101 140-144 0.0 1101 145-149 0.0 1101 150-151 0.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 12 2.0 13 22.0 14 41.0 15 47.0 16 52.0 17 41.0 18 31.0 19 38.0 20 29.0 21 23.0 22 30.0 23 37.0 24 38.0 25 37.0 26 49.0 27 41.0 28 15.0 29 21.0 30 29.0 31 35.0 32 57.0 33 91.0 34 173.0 35 598.0 36 2248.0 37 175.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 54.474999999999994 13.825000000000001 13.675 18.025 2 36.65 18.525 24.099999999999998 20.724999999999998 3 33.975 23.724999999999998 25.525 16.775000000000002 4 34.925 26.974999999999998 19.400000000000002 18.7 5 34.5 29.7 19.2 16.6 6 31.025000000000002 27.525 23.0 18.45 7 32.35 19.525000000000002 27.775 20.349999999999998 8 31.95 22.45 22.725 22.875 9 31.0 23.95 23.825 21.224999999999998 10-14 32.68 25.205 22.689999999999998 19.425 15-19 32.24 24.834999999999997 23.91 19.015 20-24 31.81 25.515 23.835 18.84 25-29 31.755 25.855 23.990000000000002 18.4 30-34 30.869999999999997 25.395 24.73 19.005 35-39 31.169999999999998 26.450000000000003 23.575 18.805 40-44 31.175000000000004 25.740000000000002 23.95 19.134999999999998 45-49 30.69 26.415 24.45 18.445 50-54 24.445 27.115000000000002 28.675 19.765 55-59 29.34 27.075 24.975 18.61 60-64 30.035 27.18 24.01 18.775 65-69 29.445 26.435 24.285 19.835 70-74 26.015 30.020000000000003 24.865000000000002 19.1 75-79 25.335 31.025000000000002 24.68 18.96 80-84 27.744999999999997 29.005 24.875 18.375 85-89 28.655 27.54 25.324999999999996 18.48 90-94 28.610000000000003 28.23 25.3 17.86 95-99 27.839999999999996 28.52 24.54 19.1 100-104 28.155 29.044999999999998 24.245 18.555 105-109 28.384999999999998 28.58 24.595 18.44 110-114 28.15 29.62 24.23 18.0 115-119 27.794999999999998 29.095 24.195 18.915000000000003 120-124 27.694999999999997 29.14 24.955 18.21 125-129 28.865000000000002 28.675 24.485 17.974999999999998 130-134 29.935000000000002 27.925 24.2 17.94 135-139 29.125 28.365000000000002 23.830000000000002 18.68 140-144 29.285 29.42 23.615 17.68 145-149 29.92 28.994999999999997 23.405 17.68 150-151 29.3375 29.562500000000004 23.400000000000002 17.7 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.5 6 2.5 7 2.5 8 1.0 9 2.0 10 2.5 11 3.5 12 4.5 13 4.0 14 4.5 15 5.0 16 3.5 17 3.0 18 4.5 19 4.0 20 2.5 21 2.0 22 1.5 23 1.5 24 1.5 25 1.5 26 3.0 27 5.5 28 7.5 29 10.0 30 14.0 31 16.5 32 19.5 33 29.5 34 31.0 35 36.5 36 53.5 37 69.0 38 83.0 39 107.0 40 127.0 41 155.0 42 186.0 43 224.5 44 245.5 45 240.5 46 228.5 47 259.0 48 260.0 49 200.5 50 168.5 51 139.0 52 138.0 53 119.5 54 72.5 55 57.5 56 59.5 57 47.0 58 32.5 59 24.0 60 20.5 61 11.5 62 13.5 63 18.5 64 12.5 65 12.0 66 13.0 67 10.5 68 9.0 69 6.0 70 7.0 71 7.5 72 5.5 73 4.5 74 5.5 75 8.0 76 5.5 77 7.0 78 11.0 79 9.5 80 7.0 81 9.5 82 11.5 83 11.0 84 12.5 85 15.0 86 16.0 87 18.0 88 20.5 89 17.0 90 11.5 91 7.5 92 8.5 93 11.5 94 10.0 95 6.5 96 4.5 97 4.0 98 3.0 99 2.5 100 34.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150-151 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 63.275000000000006 #Duplication Level Percentage of deduplicated Percentage of total 1 70.48597392335046 44.6 2 17.661003555906756 22.35 3 7.151323587514816 13.575000000000001 4 2.449624654286843 6.2 5 1.1457921770051362 3.6249999999999996 6 0.434610825760569 1.6500000000000001 7 0.39510075069142636 1.7500000000000002 8 0.19755037534571318 1.0 9 0.0 0.0 >10 0.0 0.0 >50 0.03951007506914263 2.3 >100 0.03951007506914263 2.9499999999999997 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG 118 2.9499999999999997 No Hit GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGT 92 2.3 No Hit CTGCTGTGCAAAAGATGCTTGAAATGTTAAACAATCCTGAGGTAGCCAGC 8 0.2 No Hit GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGT 8 0.2 No Hit AAAGAGACCAGAGTCACTTAGGATTTATGAAGCTCATGTCGGAATGAGTA 8 0.2 No Hit GGCGGAGCTAATGTTGTGAATCATGGATACACCAAGGGTGATGGCCTTGG 8 0.2 No Hit CTACCATGAGTAGAGAGTTTAATGCTCTTGTGGTTGCAGGGTCTGCTATA 8 0.2 No Hit ACCATGATTTTACCTCAACGAACTTCTGTACCTGATACTTCTCTCCTGAT 7 0.17500000000000002 No Hit TGATGTCTTTCAAGGCTGCTCAAGAAGATAAGACCAAAACGATTGAATCT 7 0.17500000000000002 No Hit GGCAACAGGACAAGTGGACAGGCTATTTCCCTGTGAAGTGGCACATTGTG 7 0.17500000000000002 No Hit ATGGGCTTTGGCTTCAGAAAAACTCACTTCTCTTGAGATTGGGTGTGTTT 7 0.17500000000000002 No Hit CAATGCCACCATGAGCTTTCCACAGAATTATCCAGTGAGTCCTCCAACTG 7 0.17500000000000002 No Hit GATCTTCGATTTGGGGAAGAAATGCTTGGGGCTTCATTGGGGATGTCATG 7 0.17500000000000002 No Hit GCTAGATACTAGTAGTAGTTGCAGACAGAGAGCTAGTGTTAGACAGGTTC 7 0.17500000000000002 No Hit CACCTCTGTTATCTTTATAGCTATCTTCTTGTAGTTTTTTGTCGGCTCCA 7 0.17500000000000002 No Hit TGATATGATCCGTAAAATTGCTGATGAGGATCCTGATGAGGCAAATGACA 7 0.17500000000000002 No Hit GGGCATTCGAGTTTCTTGCAAACCTTGTCTAGTATAATCTTCAGCGGTGG 7 0.17500000000000002 No Hit CTCTGGGACGACACCGTTGCTGGTCCTCTTCCGGAAAACGGCCTCGGGAA 6 0.15 No Hit TACCATCATCGATGACCTTGTATCAGTGGATTCCCTCATTGAGGAGCGTC 6 0.15 No Hit GGAAGAGGAGATTGGTGGGTTAAATTTGAAGGGTGTGGGATATCCTGGTA 6 0.15 No Hit GCTAAGCCTCTTGGTTGGAGCAAGCTGCGCTAATATAGAAGGTAAAATTG 6 0.15 No Hit CAGCAGTAGCAGCAGGAACCTACAAAGATATATGGATTTATCTGGTGGCA 6 0.15 No Hit GAGGATGAAGGAACATTGCCACCGTGCAATGGTTTACGCCACCAAAATAA 6 0.15 No Hit TCCTTCTTTTAAGTTAATATTGGGGTCGGCCTCATTTTCACGAAGGAAAA 6 0.15 No Hit GTGAAACACTGGGCCAAGTCAAGAGGAGTCAATGCAACTTACCAAGGGAC 6 0.15 No Hit GAAAAGGAGTGAGATATTCAAGAGAAATACGTTTAGAAATCAAGAGAGAG 6 0.15 No Hit AAGCGTAGTACCAGCGGGGCGCAGCGCGCGGAGGGCGGCGGCGGAGGATG 6 0.15 No Hit GCTTCGACCCACTGGGCTTGGCTGACGATCCCGAAGCATTCGCTGAGTTG 6 0.15 No Hit GCTTGTCGAGCTGTGCATATGTTACCTTTTTGGCTGGTGATGGAGACTAC 5 0.125 No Hit GAGGATGAGGGTGTTGATGACCAAACTGTCAAGGTGGTAGACATTGTTGA 5 0.125 No Hit AGAACATAGATCAATCACAATGGCTTTACTTGACTTCCTAATTCTATCAG 5 0.125 No Hit AAGTTGGATACCCTTGAAGAAGAAAGTGAATATGAAAATAAGGGCCTATC 5 0.125 No Hit TTTTTTCCTTGGATATTGGAGGAACATATTTCTGTAGTTTTTTCTTAAAG 5 0.125 No Hit GAGAACGTGATCTGGAGAATATTTGGTGCAAACTCAATGGTGCAAGTCAG 5 0.125 No Hit TCGTACTTCCGGAATCATCTTCTTTGGAAGTTCTGGAAGCCTTGATGAGA 5 0.125 No Hit GTCTGATTTCGACGCCCAAACTCCTTCAGCTTATGATCCTTTTGCTGATG 5 0.125 No Hit ATGAACTGGTAAAAAGGAGGACTTCCTATGACAATTCTCCCTACGGTTCC 5 0.125 No Hit CAGAAAGTTTGGGATTGGAAGGAAGCTACATAGACAAGGCACTGAATTTG 5 0.125 No Hit GGGCAAAGAAAACGAGATATCGGAGACACCAGGTGGGTATAAGCTAGCTG 5 0.125 No Hit GCCAGCCGAACCGAGCTTAGTTCGTTCTTTATTAAGCCAAGCTGCTTCCG 5 0.125 No Hit GGCTGTCAATATCACCATAAATAGCATTGGCACTGAGCTGACCCGGGAGG 5 0.125 No Hit GAGAAAGCTATGTAGTGGTACACAACATAGCAAAGAGACACAACGTGGGT 5 0.125 No Hit CGTCAAAGGAGGGAATTTCTCAGGTCGCAAGATTGAGCATTCGTTGAAGA 5 0.125 No Hit GAAAGTTCTTCGCCGGAAACTGAGGTCAAAGCAGCGGCTACGGAACCATT 5 0.125 No Hit GATGAGCAATAAGGCCGTCTGCTTTCCAATATCCAGATCCATTGGTGGTT 5 0.125 No Hit TACAGCGAGGATGATGTGCATAAGTGCATTAAATACAACGTCTGGGCTAG 5 0.125 No Hit TTTTTTTTTTTGTTCTCCACAGAGTCTTCCGTTCAGAAATGCAATGGATT 5 0.125 No Hit AACTACGAAACTAGAATTGGGAAGTTCAAAGGAGCTGTTGATCCTTGTAC 5 0.125 No Hit GAGCTTCAGCATAGTGAGGATTTAAGGACCTTTTTGCAGGCTGATGAGGA 5 0.125 No Hit GGAGGAGCTCAAGGTGGTCTTTGACCAATTCGACGCAAACGGTGACGGCA 5 0.125 No Hit GTCCTAGGAGAATGCATTGCATTATGGATGCCATCCCCGCTTCTGCCATT 5 0.125 No Hit ATACATACAAGAATTTTGATGCTGTTAAAGGACGGCATCATTTCGATCCC 5 0.125 No Hit GGAGGCGGCATGGGACACACTATAAAGGCAATAATCTCTAAGTATCCCCA 5 0.125 No Hit CTGTCGTCCTCCGCAACGCCGCAAGCAAGCTCGGCCATCAGCCTTTTTTA 5 0.125 No Hit CCACTTCCAGTCAAGTTGAACGATGATCTAGCTGACACCATCCTTCTGCC 5 0.125 No Hit CCTGGTCAGCTCAACTCGGACCTCCGAAAGCTTGCTGTGAATCTCATTCC 5 0.125 No Hit GTTTGATAGTAAAGATAGCCTGAGGTGCACTTACCAGATAGCTACTAGCA 5 0.125 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0125 0.0 0.0 0.0 0.0 26-27 0.025 0.0 0.0 0.0 0.0 28-29 0.025 0.0 0.0 0.0 0.0 30-31 0.025 0.0 0.0 0.0 0.0 32-33 0.025 0.0 0.0 0.0 0.0 34-35 0.025 0.0 0.0 0.0 0.0 36-37 0.025 0.0 0.0 0.0 0.0 38-39 0.025 0.0 0.0 0.0 0.0 40-41 0.025 0.0 0.0 0.0 0.0 42-43 0.025 0.0 0.0 0.0 0.0 44-45 0.025 0.0 0.0 0.0 0.0 46-47 0.025 0.0 0.0 0.0 0.0 48-49 0.037500000000000006 0.0 0.0 0.0 0.0 50-51 0.05 0.0 0.0 0.0 0.0 52-53 0.05 0.0 0.0 0.0 0.0 54-55 0.075 0.0 0.0 0.0 0.0 56-57 0.075 0.0 0.0 0.0 0.0 58-59 0.075 0.0 0.0 0.0 0.0 60-61 0.075 0.0 0.0 0.0 0.0 62-63 0.075 0.0 0.0 0.0 0.0 64-65 0.075 0.0 0.0 0.0 0.0 66-67 0.075 0.0 0.0 0.0 0.0 68-69 0.075 0.0 0.0 0.0 0.0 70-71 0.075 0.0 0.0 0.0 0.0 72-73 0.125 0.0 0.0 0.0 0.0 74-75 0.15 0.0 0.0 0.0 0.0 76-77 0.1875 0.0 0.0 0.0 0.0 78-79 0.2 0.0 0.0 0.0 0.0 80-81 0.21250000000000002 0.0 0.0 0.0 0.0 82-83 0.2375 0.0 0.0 0.0 0.0 84-85 0.35 0.0 0.0 0.0 0.0 86-87 0.4625 0.0 0.0 0.0 0.0 88-89 0.6 0.0 0.0 0.0 0.0 90-91 0.7 0.0 0.0 0.0 0.0 92-93 0.725 0.0 0.0 0.0 0.0 94-95 0.75 0.0 0.0 0.0 0.0 96-97 0.925 0.0 0.0 0.0 0.0 98-99 1.0375 0.0 0.0 0.0 0.0 100-101 1.1749999999999998 0.0 0.0 0.0 0.0 102-103 1.4 0.0 0.0 0.0 0.0 104-105 1.8125 0.0 0.0 0.0 0.0 106-107 1.9375 0.0 0.0 0.0 0.0 108-109 2.125 0.0 0.0 0.0 0.0 110-111 2.4625 0.0 0.0 0.0 0.0 112-113 2.7125000000000004 0.0 0.0 0.0 0.0 114-115 3.175 0.0 0.0 0.0 0.0 116-117 3.625 0.0 0.0 0.0 0.0 118-119 4.175 0.0 0.0 0.0 0.0 120-121 4.625 0.0 0.0 0.0 0.0 122-123 5.025 0.0 0.0 0.0 0.0 124-125 5.5 0.0 0.0 0.0 0.0 126-127 6.074999999999999 0.0 0.0 0.0 0.0 128-129 6.6625 0.0 0.0 0.0 0.0 130-131 7.35 0.0 0.0 0.0 0.0 132-133 7.800000000000001 0.0 0.0 0.0 0.0 134-135 8.5875 0.0 0.0 0.0 0.0 136-137 9.2375 0.0 0.0 0.0 0.0 138-139 10.1 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CCGGAAT 10 0.006830828 145.0 9 GTACTTC 10 0.006830828 145.0 3 ACTTCCG 10 0.006830828 145.0 5 CCGTAAT 10 0.006830828 145.0 145 ATTGCCA 10 0.006830828 145.0 2 CGTACTT 10 0.006830828 145.0 2 TCGTACT 10 0.006830828 145.0 1 GGTATTT 10 0.006830828 145.0 145 AATTGCC 10 0.006830828 145.0 1 TTGCCAA 10 0.006830828 145.0 3 >>END_MODULE Read 2629774 spots for SRR26075377.sra Written 2629774 spots for SRR26075377.sra Read 2629770 spots for SRR26075377.sra Written 2629770 spots for SRR26075377.sra Read 2629770 spots for SRR26075377.sra Written 2629770 spots for SRR26075377.sra Read 2629770 spots for SRR26075377.sra Written 2629770 spots for SRR26075377.sra Read 2629770 spots for SRR26075377.sra Written 2629770 spots for SRR26075377.sra Read 2629770 spots for SRR26075377.sra Written 2629770 spots for SRR26075377.sra Read 2629770 spots for SRR26075377.sra Written 2629770 spots for SRR26075377.sra Read 2629770 spots for SRR26075377.sra Written 2629770 spots for SRR26075377.sra Read 2629770 spots for SRR26075377.sra Written 2629770 spots for SRR26075377.sra Read 2629770 spots for SRR26075377.sra Written 2629770 spots for SRR26075377.sra Read 2629770 spots for SRR26075377.sra Written 2629770 spots for SRR26075377.sra Read 2629770 spots for SRR26075377.sra Written 2629770 spots for SRR26075377.sra Read 2629770 spots for SRR26075377.sra Written 2629770 spots for SRR26075377.sra Read 2629770 spots for SRR26075377.sra Written 2629770 spots for SRR26075377.sra Read 2629770 spots for SRR26075377.sra Written 2629770 spots for SRR26075377.sra Read 2629770 spots for SRR26075377.sra Written 2629770 spots for SRR26075377.sra Read 2629770 spots for SRR26075377.sra Written 2629770 spots for SRR26075377.sra Read 2629770 spots for SRR26075377.sra Written 2629770 spots for SRR26075377.sra Read 2629770 spots for SRR26075377.sra Written 2629770 spots for SRR26075377.sra Read 2629770 spots for SRR26075377.sra Written 2629770 spots for SRR26075377.sra SRR ids: ['SRR26075377.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_dyaxr2wg SRR26075377.sra spots: 52595404 blocks: [[1, 2629770], [2629771, 5259540], [5259541, 7889310], [7889311, 10519080], [10519081, 13148850], [13148851, 15778620], [15778621, 18408390], [18408391, 21038160], [21038161, 23667930], [23667931, 26297700], [26297701, 28927470], [28927471, 31557240], [31557241, 34187010], [34187011, 36816780], [36816781, 39446550], [39446551, 42076320], [42076321, 44706090], [44706091, 47335860], [47335861, 49965630], [49965631, 52595404]] SRR26075377 file size 19428153 SRR26075377 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o SRR26075377 SRR26075377_1.fastq SRR26075377_2.fastq Input file: SRR26075377_1.fastq Paired file: SRR26075377_2.fastq trimmed: SRR26075377-trimmed-pair1.fastq, SRR26075377-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Tue Feb 11 23:26:16 2025 >> started Tue Feb 11 23:27:18 2025 >> done (61.929s) 52595404 read pairs processed; of these: 573 ( 0.00%) short read pairs filtered out after trimming by size control 1466645 ( 2.79%) empty read pairs filtered out after trimming by size control 51128186 (97.21%) read pairs available; of these: 7720528 (15.10%) trimmed read pairs available after processing 43407658 (84.90%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 18 41 0.00% 19 80 0.00% 20 91 0.00% 21 128 0.00% 22 208 0.00% 23 242 0.00% 24 275 0.00% 25 325 0.00% 26 323 0.00% 27 328 0.00% 28 310 0.00% 29 299 0.00% 30 351 0.00% 31 310 0.00% 32 314 0.00% 33 317 0.00% 34 361 0.00% 35 341 0.00% 36 382 0.00% 37 360 0.00% 38 435 0.00% 39 514 0.00% 40 399 0.00% 41 448 0.00% 42 522 0.00% 43 488 0.00% 44 608 0.00% 45 562 0.00% 46 611 0.00% 47 687 0.00% 48 662 0.00% 49 793 0.00% 50 974 0.00% 51 931 0.00% 52 1077 0.00% 53 1210 0.00% 54 1256 0.00% 55 1382 0.00% 56 1341 0.00% 57 1429 0.00% 58 1654 0.00% 59 1832 0.00% 60 2117 0.00% 61 2579 0.01% 62 2951 0.01% 63 3422 0.01% 64 3747 0.01% 65 3662 0.01% 66 3970 0.01% 67 4009 0.01% 68 4379 0.01% 69 4864 0.01% 70 5933 0.01% 71 7308 0.01% 72 8420 0.02% 73 10022 0.02% 74 10670 0.02% 75 11263 0.02% 76 11190 0.02% 77 12134 0.02% 78 12301 0.02% 79 13820 0.03% 80 15726 0.03% 81 18024 0.04% 82 21775 0.04% 83 24599 0.05% 84 26726 0.05% 85 27736 0.05% 86 28030 0.05% 87 28114 0.05% 88 28129 0.06% 89 30163 0.06% 90 32021 0.06% 91 36233 0.07% 92 40694 0.08% 93 46379 0.09% 94 50292 0.10% 95 53161 0.10% 96 53156 0.10% 97 53314 0.10% 98 51477 0.10% 99 53337 0.10% 100 54920 0.11% 101 58004 0.11% 102 65098 0.13% 103 71413 0.14% 104 77446 0.15% 105 81529 0.16% 106 82937 0.16% 107 82102 0.16% 108 81858 0.16% 109 80767 0.16% 110 79458 0.16% 111 85519 0.17% 112 90119 0.18% 113 96897 0.19% 114 106525 0.21% 115 113782 0.22% 116 116134 0.23% 117 115140 0.23% 118 114082 0.22% 119 113096 0.22% 120 111818 0.22% 121 113393 0.22% 122 117555 0.23% 123 127088 0.25% 124 136445 0.27% 125 144189 0.28% 126 149506 0.29% 127 149851 0.29% 128 146822 0.29% 129 146445 0.29% 130 141534 0.28% 131 140049 0.27% 132 145561 0.28% 133 152857 0.30% 134 160999 0.31% 135 174960 0.34% 136 176945 0.35% 137 180165 0.35% 138 182311 0.36% 139 176497 0.35% 140 174458 0.34% 141 173573 0.34% 142 172284 0.34% 143 175661 0.34% 144 185413 0.36% 145 191135 0.37% 146 202533 0.40% 147 207880 0.41% 148 207501 0.41% 149 203958 0.40% 150 201268 0.39% 151 43407658 84.90% 51128186 reads passed initial QC criterion=sequence-density sequence-density=1.21 sequence-density-rank=1 fanout-score=23.87 fanout-score-rank=2 prefix-density=1.30 prefix-fanout=22.2 sequence=GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCTCACATCTCGTATGCCGTCTTCTGCTTG criterion=fanout-score sequence-density=0.03 sequence-density-rank=29 fanout-score=34.50 fanout-score-rank=1 prefix-density=0.11 prefix-fanout=8.8 sequence=CACCTCCACCACTTCCGCGGGATTGAGCTTCGTTCACGGTGATGTTACGGCCATCGAGGTCCTGACCGTTCATTCCATCAATAGCATCTCTCATTGCCTTCTC criterion=sequence-density sequence-density=0.39 sequence-density-rank=1 fanout-score=2.54 fanout-score-rank=33 prefix-density=0.39 prefix-fanout=2.5 sequence=ATGTACCCTGAC criterion=fanout-score sequence-density=0.01 sequence-density-rank=37 fanout-score=114.73 fanout-score-rank=1 prefix-density=0.10 prefix-fanout=7.5 sequence=ATTTTCTTTGAGAGTGCATAGATTTGTGTTGATATAGAAAACAATGGCACTACATGGAAAGATTGAGACAACATTAGAACTCAAGTCCTCCGCAGAGAAGTTCTACAAAGTGTGGAGGAGCCAGTCCTTCCATGTTCCCAAACATGCTTCCAAGCATATCCAAGGAGTTGATATACATGCAGGTGACTGGGAGACTGCGGGCTCTATCAGGATTTGGCAGTACACAATCGGAGGGAAAGCCGGGGTCTTTAAAGAGGAGGTTTCCTTCGATGATGAGAACAAGATCATAACTCTTAATGGTTTGGAAGGAGATGTCATGAAAATTTACAAGGTCTATAGGCCCGTCTGGCAGCTTACACCAAAAGGCTCGGGCTGCTTGGCAAAACTGACCATTGAATACGAAAAACTCCATCCTGAAGTCCCGGTTCCAGAGATTTATGTTGATCTTATGGTTCATATGACTAAAGACATCGACGAAGCCCTTAGCACGGAGTAATAGAAGGGGTCATCG SRR26075377 testing PE reads STAR mapping to Ensembl genome Started job on | Feb 11 23:28:03 Started mapping on | Feb 11 23:28:04 Finished on | Feb 11 23:39:05 Mapping speed, Million of reads per hour | 278.46 Number of input reads | 51128186 Average input read length | 294 UNIQUE READS: Uniquely mapped reads number | 40637459 Uniquely mapped reads % | 79.48% Average mapped length | 291.29 Number of splices: Total | 37160928 Number of splices: Annotated (sjdb) | 36225331 Number of splices: GT/AG | 36473147 Number of splices: GC/AG | 519564 Number of splices: AT/AC | 38173 Number of splices: Non-canonical | 130044 Mismatch rate per base, % | 0.41% Deletion rate per base | 0.03% Deletion average length | 3.09 Insertion rate per base | 0.02% Insertion average length | 2.63 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 1074932 % of reads mapped to multiple loci | 2.10% Number of reads mapped to too many loci | 198661 % of reads mapped to too many loci | 0.39% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 16.00% % of reads unmapped: other | 2.03% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 9415795 9415795 9415795 N_multimapping 1074932 1074932 1074932 N_noFeature 1037412 39968469 1487317 N_ambiguous 449272 3470 227667 UnstrandedReadsAssigned:39150775 PositiveStrandReadsAssigned:665520 NegativeStrandReadsAssigned:38922475 Dataset is classified negative stranded MeadianReadLen=151 20thPercentileLength=151 echo kmer=147 SRR26075377 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,400 [index] number of k-mers: 62,057,036 [index] number of equivalence classes: 130,681 [quant] running in paired-end mode [quant] will process pair 1: SRR26075377-trimmed-pair1.fastq SRR26075377-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 51,128,186 reads, 40,961,725 reads pseudoaligned [quant] estimated average fragment length: 211.665 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,236 rounds 52401 SRR26075377.ke.tsv 34699 SRR26075377.se.tsv 87100 total ==> SRR26075377.ke.tsv <== target_id length eff_length est_counts tpm Potri.005G200100.1.v4.1 2018 1807.33 4513 51.6594 Potri.005G024800.1.v4.1 1035 824.335 2697 67.6862 Potri.004G059700.1.v4.1 961 750.34 81 2.23331 Potri.007G009000.2.v4.1 1416 1205.33 0 0 Potri.003G141000.2.v4.1 2943 2732.33 1677.37 12.7004 Potri.016G087400.1.v4.1 270 92.5591 4554 1017.88 Potri.015G069301.1.v4.1 564 355.931 0 0 Potri.010G195200.1.v4.1 1773 1562.33 441 5.83965 Potri.012G127500.1.v4.1 977 766.335 24649 665.432 ==> SRR26075377.se.tsv <== Potri.001G166300.v4.1 0 Potri.001G448400.v4.1 283 Potri.001G233950.v4.1 0 Potri.001G122700.v4.1 470 Potri.001G212900.v4.1 0 Potri.001G182400.v4.1 0 Potri.001G256600.v4.1 0 Potri.001G040500.v4.1 10 Potri.001G416900.v4.1 0 Potri.001G452600.v4.1 1056 SRR26075377 completed mapping pipeline successfully