Starting /dee2/code/volunteer_pipeline.sh SRR26075380 current disk space = 3052048674816 free memory = 1446716268 SRR26075380 SRAfilesize 18f60c85b91ec387baf726b6b473102e SRR26075380.sra SRR26075380.sra file validated SRR26075380 is paired end SRR26075380 is conventional basespace SRR26075380 read1 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR26075380_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.7345 37.0 37.0 37.0 37.0 37.0 2 36.6505 37.0 37.0 37.0 37.0 37.0 3 36.711 37.0 37.0 37.0 37.0 37.0 4 36.7665 37.0 37.0 37.0 37.0 37.0 5 36.7515 37.0 37.0 37.0 37.0 37.0 6 36.747 37.0 37.0 37.0 37.0 37.0 7 36.6735 37.0 37.0 37.0 37.0 37.0 8 36.741 37.0 37.0 37.0 37.0 37.0 9 36.71 37.0 37.0 37.0 37.0 37.0 10-14 36.655649999999994 37.0 37.0 37.0 37.0 37.0 15-19 36.643 37.0 37.0 37.0 37.0 37.0 20-24 36.5914 37.0 37.0 37.0 37.0 37.0 25-29 36.4825 37.0 37.0 37.0 37.0 37.0 30-34 36.4397 37.0 37.0 37.0 37.0 37.0 35-39 36.4134 37.0 37.0 37.0 37.0 37.0 40-44 36.3142 37.0 37.0 37.0 37.0 37.0 45-49 36.1899 37.0 37.0 37.0 37.0 37.0 50-54 36.1787 37.0 37.0 37.0 37.0 37.0 55-59 35.9714 37.0 37.0 37.0 37.0 37.0 60-64 35.9477 37.0 37.0 37.0 37.0 37.0 65-69 35.8927 37.0 37.0 37.0 37.0 37.0 70-74 35.9562 37.0 37.0 37.0 37.0 37.0 75-79 36.0951 37.0 37.0 37.0 37.0 37.0 80-84 36.0711 37.0 37.0 37.0 37.0 37.0 85-89 36.0027 37.0 37.0 37.0 37.0 37.0 90-94 35.9423 37.0 37.0 37.0 37.0 37.0 95-99 35.909000000000006 37.0 37.0 37.0 37.0 37.0 100-104 35.8935 37.0 37.0 37.0 37.0 37.0 105-109 35.9289 37.0 37.0 37.0 37.0 37.0 110-114 35.73100000000001 37.0 37.0 37.0 37.0 37.0 115-119 35.6774 37.0 37.0 37.0 37.0 37.0 120-124 35.692699999999995 37.0 37.0 37.0 37.0 37.0 125-129 35.50170000000001 37.0 37.0 37.0 37.0 37.0 130-134 35.4313 37.0 37.0 37.0 37.0 37.0 135-139 35.31269999999999 37.0 37.0 37.0 32.2 37.0 140-144 35.1981 37.0 37.0 37.0 29.8 37.0 145-149 35.17059999999999 37.0 37.0 37.0 29.8 37.0 150-151 34.95225 37.0 37.0 37.0 25.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.0 1101 2 0.0 1101 3 0.0 1101 4 0.0 1101 5 0.0 1101 6 0.0 1101 7 0.0 1101 8 0.0 1101 9 0.0 1101 10-14 0.0 1101 15-19 0.0 1101 20-24 0.0 1101 25-29 0.0 1101 30-34 0.0 1101 35-39 0.0 1101 40-44 0.0 1101 45-49 0.0 1101 50-54 0.0 1101 55-59 0.0 1101 60-64 0.0 1101 65-69 0.0 1101 70-74 0.0 1101 75-79 0.0 1101 80-84 0.0 1101 85-89 0.0 1101 90-94 0.0 1101 95-99 0.0 1101 100-104 0.0 1101 105-109 0.0 1101 110-114 0.0 1101 115-119 0.0 1101 120-124 0.0 1101 125-129 0.0 1101 130-134 0.0 1101 135-139 0.0 1101 140-144 0.0 1101 145-149 0.0 1101 150-151 0.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 17 1.0 18 1.0 19 1.0 20 2.0 21 5.0 22 5.0 23 2.0 24 6.0 25 8.0 26 7.0 27 16.0 28 18.0 29 20.0 30 30.0 31 39.0 32 42.0 33 125.0 34 150.0 35 386.0 36 2904.0 37 232.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 39.89494747373687 17.158579289644823 9.479739869934969 33.46673336668334 2 16.975 14.299999999999999 37.025000000000006 31.7 3 16.075 19.8 30.275000000000002 33.85 4 21.625 24.975 25.05 28.349999999999998 5 23.325000000000003 31.225 24.5 20.95 6 23.175 31.55 23.599999999999998 21.675 7 14.875 28.999999999999996 41.375 14.75 8 16.825000000000003 29.175 33.025 20.974999999999998 9 16.150000000000002 28.449999999999996 33.925 21.475 10-14 19.35596779838992 32.43162158107906 26.86134306715336 21.35106755337767 15-19 19.21 28.970000000000002 28.27 23.549999999999997 20-24 20.47 29.285 28.975 21.27 25-29 19.040000000000003 29.659999999999997 28.33 22.97 30-34 19.81 29.37 27.07 23.75 35-39 18.95 29.37 28.33 23.35 40-44 19.1 30.035 27.61 23.255 45-49 19.830000000000002 29.23 27.67 23.27 50-54 20.44 27.825 27.935 23.799999999999997 55-59 19.465 29.625 27.450000000000003 23.46 60-64 20.66 28.175 27.935 23.23 65-69 19.72 28.835 28.225 23.22 70-74 21.535 27.62 27.839999999999996 23.005 75-79 21.060000000000002 27.700000000000003 28.28 22.96 80-84 20.8 28.065 27.82 23.315 85-89 20.805 27.49 27.810000000000002 23.895 90-94 20.979999999999997 27.589999999999996 28.189999999999998 23.24 95-99 20.880000000000003 26.825 28.32 23.974999999999998 100-104 20.53 27.245 28.83 23.395 105-109 22.134999999999998 27.48 27.77 22.615 110-114 21.52 27.875 26.974999999999998 23.630000000000003 115-119 20.215 28.725 27.33 23.73 120-124 21.035 28.000000000000004 27.465 23.5 125-129 22.305 28.1 26.650000000000002 22.945 130-134 22.59 28.375 25.88 23.155 135-139 21.69 28.21 26.235000000000003 23.865 140-144 21.945 28.34 25.014999999999997 24.7 145-149 22.445 27.584999999999997 25.64 24.33 150-151 22.925 27.275 25.0375 24.762500000000003 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 0.0 1 0.5 2 1.0 3 1.0 4 1.0 5 1.0 6 1.0 7 1.5 8 2.0 9 1.0 10 0.0 11 1.5 12 3.5 13 2.0 14 1.0 15 1.0 16 0.5 17 0.5 18 2.0 19 2.5 20 1.0 21 2.5 22 4.0 23 4.0 24 6.0 25 10.5 26 7.5 27 5.5 28 11.5 29 21.5 30 29.5 31 32.0 32 44.0 33 54.5 34 56.0 35 67.0 36 115.5 37 127.0 38 109.0 39 149.5 40 186.0 41 205.0 42 250.5 43 268.5 44 270.0 45 277.0 46 263.5 47 252.0 48 208.0 49 185.0 50 161.5 51 117.5 52 104.0 53 79.0 54 58.0 55 53.5 56 40.0 57 24.0 58 16.0 59 11.0 60 11.5 61 12.0 62 7.5 63 3.5 64 4.0 65 6.0 66 9.0 67 13.0 68 9.0 69 2.5 70 2.5 71 3.0 72 2.5 73 1.0 74 0.5 75 0.0 76 0.0 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.05 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.005 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150-151 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 59.050000000000004 #Duplication Level Percentage of deduplicated Percentage of total 1 63.63251481795089 37.574999999999996 2 19.89839119390347 23.5 3 9.271803556308214 16.425 4 3.6409822184589333 8.6 5 1.5241320914479255 4.5 6 0.9737510584250635 3.45 7 0.3810330228619814 1.575 8 0.2540220152413209 1.2 9 0.12701100762066045 0.675 >10 0.29635901778154106 2.5 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCATAACATCTCGTAT 28 0.7000000000000001 TruSeq Adapter, Index 6 (97% over 37bp) CCCACCTTTGGTACAAGGATATCCATAGCAGCCACTGTCTTTGAATCATC 15 0.375 No Hit ACTTGGTGGCAGCAAGGAAGGCGTTGTAGGAGGCTAAATAGCTAACTCCA 12 0.3 No Hit GTTTGTACCGGAAGGCATGGATGTAGCAGTGAAAGTCACTGTATAAGTCT 12 0.3 No Hit GTGATCAAAACCCTAAACAATCTTACATCAAATTACAAGCACGTATGGTC 12 0.3 No Hit ATTTCCTTGAGTTCCTTTAGGAGGGTCTTCTCAACAGCATTTTCAACGAT 11 0.27499999999999997 No Hit ATCAAGCTTTTAAATGCACAGCATGAGGAAGTACTGCATGCAAACAGATT 10 0.25 No Hit CCTGAGGCCAGGGAAGCCCATGTCCTTTGAGAGACTGTAAACAATGTGTA 9 0.22499999999999998 No Hit GTGTCAGCTTAAGCTTCCCAGCCACCACTGCAAATCTCTACAATCTAAAG 9 0.22499999999999998 No Hit CTTCAGCTTCCCACAGTATCCCATTCTCAATTTCCTTGTAGGGGAACGAG 9 0.22499999999999998 No Hit GTGGGAGCTAGTTTCTTTGGAATACCAGCAGCCTTGAGTTCTTCGAGAGA 8 0.2 No Hit GCCCACAATAACTCCTGCCTTCACAGTTGACATTACACACACACACACAC 8 0.2 No Hit GTTGGGATCATCGGCTAAATTTGTGAAAGCAAGGCAGTTCCACAACCTGA 8 0.2 No Hit GCTCGTTAAGACTGATTTTAGTTTTGTTGAGCCACAAGCTGATCAATCTG 8 0.2 No Hit GCTGCATGATCCAATGATAGGCTCAGTAGGGTGGAAGACACACTCATTGA 8 0.2 No Hit GCTCGTCACTTGAAGGCCTATCTGCAGGCATTTCTGATTGACAAACAACA 8 0.2 No Hit AGCAGCTACAAATTCTCCATAGTCGATTGTTCCACTGTTGTCCACATCAG 7 0.17500000000000002 No Hit GCCATCTTTATCAGCACCCACAGTTTTGTCCACTAATTTTCCATCTTTCA 7 0.17500000000000002 No Hit ACCAGAATCAATAACACCCCGAGGACAACCAACTTGATCTTCATGTTTTG 7 0.17500000000000002 No Hit GCCTTTATTAAAGCTGGTAGACAAAGTTTGGATAAAGAAACATCACTTAT 7 0.17500000000000002 No Hit CCCCAAGCTACTAATTCCAGTCGAACCAATCTCATGTATCTTACCTTTCA 7 0.17500000000000002 No Hit GTACTATAAAAACTGTCATCAAGGCAATCCAGCCAGCATAGAATAGGAAT 7 0.17500000000000002 No Hit ATGCCCCATTCATCTCGGTCATAGCACGTAGCTGTTCACTTTCATCTCCA 7 0.17500000000000002 No Hit GGAAAATGAATAGTAACTAGGAAAACACCACCAGCATAAGGGCTATCTGG 7 0.17500000000000002 No Hit GATCTTTCTTCTGTTTGCTTTCTGCCTTCTTTAAGGAAATCATCATCGTC 7 0.17500000000000002 No Hit CACCTCTTACCTGCATTGGCGATTTGTAGCAGTAATATGTTCTGCTGGTA 6 0.15 No Hit GTTGGGGATATGATAGGCTTGGGTCTTCCAGACACCGAAGAACTTCTCAG 6 0.15 No Hit GCTAGCTAGAGGGTTCAGAAACAATTAATGGCCATTTCTAATTAGAATAG 6 0.15 No Hit GTTCCATAAAACTGCTTGGTTGGAGCCATGCGGCGACGTTTTCTCATTTG 6 0.15 No Hit GCAATTGAAAACCATTAAGGATCATACATCATCCACACATTATGCAGTTG 6 0.15 No Hit GGTTTTTTAGGGAAGGGGAAAGGAGTTTTGAGAGAGGAGAGAAAGGGCAG 6 0.15 No Hit CATCTATATCCATGCTCCTGCTTCACAGCCGAAAAAACACCGTGCTTATT 6 0.15 No Hit CTCGGTTTCGCTTCTCGGCTCTCTGTCTCTCCTCCAGACTGCTCTTCTCA 6 0.15 No Hit ACAACTCCCTGCACCAGGATTGGTCCTAGGCGACAAGTTGACTTGCTCAA 6 0.15 No Hit GCCCACTTGAAGAGTTTCCAGTAAATTTTCAGTCATTTCCTCTTACCTCA 6 0.15 No Hit ATCAAAAGAAAAGAAAGGTGCAGATTATGCTGCTAGTCAAAATTTTGATC 6 0.15 No Hit CAGCAGCATAGTAAACGTAAAGCTTTCCTCCTAAGACACAAGTCTTCGCA 6 0.15 No Hit GCAAGAGGAGGAATCCGGGGTGAGGTCACTGAAATTTCACAACCACACAG 6 0.15 No Hit CTAGAGGTGGCTGCTACTGTAGATGCACCAAAAACATGCTTTGCTAGCTG 6 0.15 No Hit CTTTGGCTTTGGTTTTGGTTTTGGAGACGGGCAACCCCCTCCACAGGCCG 6 0.15 No Hit GTCCTTTTCAGTCCTGTCATACTCCGTTATGGCAGTGTGACACTCCTGAC 6 0.15 No Hit ATCTGGCTACCAATCCTCACTGCAACTCCCCGACAGGTGCCTCATTGAGC 6 0.15 No Hit GAACTATTACTGAGAGACACCAAGCCACTTTGTGAAATTCTCAAACTCTG 6 0.15 No Hit CAATCAAAATTCAAACATAGACAATTGAACCATCGAGAAGCTGACCAGGC 6 0.15 No Hit GTAATCCTCCCACTTTTACTTGCAGCTTTCCTTGCATTGAATTGATAGCC 6 0.15 No Hit CTTCCATCTTGGACCTGAGAATTTCTTTTGGTAGAGCTTGTATGCTAGGC 6 0.15 No Hit ACCGCTCAAATCAGTCAACCTTCAGAATTCACGAATGCTGGAAGGGAAGC 6 0.15 No Hit ATCATTAAACCATCCATAAACCAGCATAACAAAGTTCACAACTTTAACAT 6 0.15 No Hit TGCAGATGGAAGCGTCCCTTGCCACGGGCTAAATCCCTTTACAGATAGCA 5 0.125 No Hit GTATTCTCCAGTCCCCCAACATTAAATAAATGTCTTCCAAGATAAGCAGC 5 0.125 No Hit GAAGAAATCATAATAATGGGGTTTCACCAGTAAGGTAACAGAGTCACAGG 5 0.125 No Hit TATGAACTCCAGAGGTGGAAATGTACTGTTTTCTGTCTGACTGATGTTAG 5 0.125 No Hit CTCGGGGGTCTCGTCAGTGGCATAGCCAAACATATGGCCTTGATCACCAG 5 0.125 No Hit GGACACATTACAATCACAATTTACCATCCACCAGCTTCAACAGTATGACA 5 0.125 No Hit GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCATAACATCGCGTAT 5 0.125 TruSeq Adapter, Index 6 (97% over 37bp) ATAGCATGGTTCTTTCAATGAGATATCGTCTATAAATTAGTAGTCGTTTC 5 0.125 No Hit CTGAAACTTCAAAATATTGAAACTTGGAATCACAGAACTTCTTGTTGTTG 5 0.125 No Hit GCCTAATCTGCTGAAGTATCTCACTTGCAGTCCTTTGGTCACCAATTGCC 5 0.125 No Hit GTCGCGCTCCTGCTTCGACAAAGTAAAGTATAGTTATCGCAGAAGATGTG 5 0.125 No Hit CTTGAGAAACTTCAATTCTGGGTGCCATCAAAATACGAATCCTTTTTGCT 5 0.125 No Hit GGTGTAATTATCAGTGGTCTTGACATCCACATGGGCTTCAATCAGTGTTT 5 0.125 No Hit CTTCAGGGTTGTACAGCTTCTAGTAGTTTCTCATAAACCTGTCTAGCAGA 5 0.125 No Hit CATCATGTACCTTAAACTCACCCCAGTTTGTTTCATGCAACCCCATCTCT 5 0.125 No Hit GTTGATTTTAGCCAGGATGTTTGGTTCCTGCTTTGAAGAGCCTCGCCGGT 5 0.125 No Hit CAAAAAATCAAAAAAGAAACAAGGAAATAAAGAAGGATGTGAAAGTGGAA 5 0.125 No Hit GTGTGTGTGTGTGTAAATAAAATTCTATCGCCTCTGGAAGTTGAAAGCCT 5 0.125 No Hit CCCTGCTTCTGGCTTGCCTGTTTGGTAATTACATGATGGAGCAAGAAGGG 5 0.125 No Hit ACACCATCAACCATATCAATATAGATTCACCACTAAGAAGATTATACTAA 5 0.125 No Hit CCACGGTACCCCGGCTCAATCACATAATCACCAAGCACCAGTGCCCCAGG 5 0.125 No Hit CTAGTTGGAAGAGTATACATGCCAGCATAAACCGCTGTCCTGACAGCCCA 5 0.125 No Hit TCCATTTCTTATCTTTAATAGTGCCAGTTCCATAGTAGCTCTTGCATCTT 5 0.125 No Hit CTTGATTCGTTCAAACACACTTGGTCCTTTAACTTCATCCATGGGGGTAT 5 0.125 No Hit CTCCTCTTTAAAGACCCCGGCTTTCCCTCCGATTGTGTACTGCCAAATCC 5 0.125 No Hit ATTGGCTTAAGCTCTGAACATGCTGAAGGCAAACTTGAACAGCATCATGC 5 0.125 No Hit ATCAACATCATTAGCTTTTGTGTCGGTAGATGTGATTGTGGTGAAGACTT 5 0.125 No Hit TCCTTCTCGATCTGCTGAATCTCTGCCGAGAACTCATAAAATGCCTCGAT 5 0.125 No Hit GTTTAGACCATCTTTCCGGTTCTTCTTGGTCTTCCTGCCTCAAAAACGCG 5 0.125 No Hit CGACCGCCTGTAGCCCCATACAAGCAAGTATTCCCGACAATGGTGGCGTC 5 0.125 No Hit CCCGCGCGGCAAACCGCCCGGGAATGTTTTTCGGGGCAATGAGCTCGACA 5 0.125 No Hit GTTTAACACAATCATCTCCTTTCAATCATTATCACAGGGCAACCTACATG 5 0.125 No Hit CCCATAACGAGAGAACCGGGAACCAAACAAGCTTGACTAGAAACTAAAGT 5 0.125 No Hit CGATTAAAGATACATAATTCCATGGAATGGAACCAACAAAGCAGCAGGAA 5 0.125 No Hit CATCTTATTGATTAAGTCATTAATCTGATTCTCACGATTCCCAGTGTCTC 5 0.125 No Hit GGGGGGATCAACAGTTAAATGAGTTATCCTATCATCATTGGTGTGGCTCC 5 0.125 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.025 0.0 0.0 0.0 0.0 52-53 0.05 0.0 0.0 0.0 0.0 54-55 0.05 0.0 0.0 0.0 0.0 56-57 0.075 0.0 0.0 0.0 0.0 58-59 0.075 0.0 0.0 0.0 0.0 60-61 0.075 0.0 0.0 0.0 0.0 62-63 0.075 0.0 0.0 0.0 0.0 64-65 0.075 0.0 0.0 0.0 0.0 66-67 0.075 0.0 0.0 0.0 0.0 68-69 0.0875 0.0 0.0 0.0 0.0 70-71 0.1 0.0 0.0 0.0 0.0 72-73 0.1 0.0 0.0 0.0 0.0 74-75 0.1 0.0 0.0 0.0 0.0 76-77 0.1375 0.0 0.0 0.0 0.0 78-79 0.1875 0.0 0.0 0.0 0.0 80-81 0.2 0.0 0.0 0.0 0.0 82-83 0.225 0.0 0.0 0.0 0.0 84-85 0.25 0.0 0.0 0.0 0.0 86-87 0.2875 0.0 0.0 0.0 0.0 88-89 0.42500000000000004 0.0 0.0 0.0 0.0 90-91 0.48750000000000004 0.0 0.0 0.0 0.0 92-93 0.6125 0.0 0.0 0.0 0.0 94-95 0.7124999999999999 0.0 0.0 0.0 0.0 96-97 0.775 0.0 0.0 0.0 0.0 98-99 1.0875 0.0 0.0 0.0 0.0 100-101 1.3625 0.0 0.0 0.0 0.0 102-103 1.7374999999999998 0.0 0.0 0.0 0.0 104-105 2.075 0.0 0.0 0.0 0.0 106-107 2.7 0.0 0.0 0.0 0.0 108-109 3.35 0.0 0.0 0.0 0.0 110-111 3.825 0.0 0.0 0.0 0.0 112-113 4.425000000000001 0.0 0.0 0.0 0.0 114-115 4.975 0.0 0.0 0.0 0.0 116-117 5.9875 0.0 0.0 0.0 0.0 118-119 6.7125 0.0 0.0 0.0 0.0 120-121 7.699999999999999 0.0 0.0 0.0 0.0 122-123 8.3625 0.0 0.0 0.0 0.0 124-125 9.3 0.0 0.0 0.0 0.0 126-127 10.037500000000001 0.025 0.0 0.0 0.0 128-129 11.1 0.05 0.0 0.0 0.0 130-131 12.0 0.05 0.0 0.0 0.0 132-133 13.2125 0.05 0.0 0.0 0.0 134-135 14.425 0.05 0.0 0.0 0.0 136-137 15.5125 0.05 0.0 0.0 0.0 138-139 16.675 0.05 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TGGTACA 10 0.006830828 145.0 9 TCGGATG 10 0.006830828 145.0 145 AATAGTT 10 0.006830828 145.0 9 GGGGGAA 10 0.006830828 145.0 4 TTTGGTA 10 0.006830828 145.0 7 TTGGTAC 10 0.006830828 145.0 8 GGGAATA 10 0.006830828 145.0 6 GGAATAG 10 0.006830828 145.0 7 >>END_MODULE SRR26075380 read2 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR26075380_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.42075 37.0 37.0 37.0 37.0 37.0 2 36.5505 37.0 37.0 37.0 37.0 37.0 3 36.4285 37.0 37.0 37.0 37.0 37.0 4 36.488 37.0 37.0 37.0 37.0 37.0 5 36.526 37.0 37.0 37.0 37.0 37.0 6 36.37 37.0 37.0 37.0 37.0 37.0 7 36.419 37.0 37.0 37.0 37.0 37.0 8 36.3625 37.0 37.0 37.0 37.0 37.0 9 36.419 37.0 37.0 37.0 37.0 37.0 10-14 36.285700000000006 37.0 37.0 37.0 37.0 37.0 15-19 36.2367 37.0 37.0 37.0 37.0 37.0 20-24 36.17550000000001 37.0 37.0 37.0 37.0 37.0 25-29 36.071 37.0 37.0 37.0 37.0 37.0 30-34 35.932100000000005 37.0 37.0 37.0 37.0 37.0 35-39 35.8753 37.0 37.0 37.0 37.0 37.0 40-44 35.8483 37.0 37.0 37.0 37.0 37.0 45-49 35.7262 37.0 37.0 37.0 37.0 37.0 50-54 35.6297 37.0 37.0 37.0 37.0 37.0 55-59 35.7054 37.0 37.0 37.0 37.0 37.0 60-64 35.7495 37.0 37.0 37.0 37.0 37.0 65-69 35.687799999999996 37.0 37.0 37.0 37.0 37.0 70-74 35.5947 37.0 37.0 37.0 37.0 37.0 75-79 35.5193 37.0 37.0 37.0 37.0 37.0 80-84 35.647800000000004 37.0 37.0 37.0 37.0 37.0 85-89 35.6548 37.0 37.0 37.0 37.0 37.0 90-94 35.6062 37.0 37.0 37.0 37.0 37.0 95-99 35.6435 37.0 37.0 37.0 37.0 37.0 100-104 35.6308 37.0 37.0 37.0 37.0 37.0 105-109 35.623099999999994 37.0 37.0 37.0 37.0 37.0 110-114 35.5312 37.0 37.0 37.0 37.0 37.0 115-119 35.5311 37.0 37.0 37.0 37.0 37.0 120-124 35.4422 37.0 37.0 37.0 37.0 37.0 125-129 35.384299999999996 37.0 37.0 37.0 37.0 37.0 130-134 35.2635 37.0 37.0 37.0 34.6 37.0 135-139 35.17975 37.0 37.0 37.0 32.2 37.0 140-144 35.21275 37.0 37.0 37.0 32.2 37.0 145-149 35.179050000000004 37.0 37.0 37.0 32.2 37.0 150-151 35.011625 37.0 37.0 37.0 25.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.0 1101 2 0.0 1101 3 0.0 1101 4 0.0 1101 5 0.0 1101 6 0.0 1101 7 0.0 1101 8 0.0 1101 9 0.0 1101 10-14 0.0 1101 15-19 0.0 1101 20-24 0.0 1101 25-29 0.0 1101 30-34 0.0 1101 35-39 0.0 1101 40-44 0.0 1101 45-49 0.0 1101 50-54 0.0 1101 55-59 0.0 1101 60-64 0.0 1101 65-69 0.0 1101 70-74 0.0 1101 75-79 0.0 1101 80-84 0.0 1101 85-89 0.0 1101 90-94 0.0 1101 95-99 0.0 1101 100-104 0.0 1101 105-109 0.0 1101 110-114 0.0 1101 115-119 0.0 1101 120-124 0.0 1101 125-129 0.0 1101 130-134 0.0 1101 135-139 0.0 1101 140-144 0.0 1101 145-149 0.0 1101 150-151 0.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 13 3.0 14 9.0 15 14.0 16 8.0 17 13.0 18 3.0 19 5.0 20 8.0 21 9.0 22 11.0 23 7.0 24 17.0 25 17.0 26 15.0 27 15.0 28 14.0 29 11.0 30 9.0 31 22.0 32 41.0 33 47.0 34 122.0 35 460.0 36 2848.0 37 272.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 46.58664666166541 22.18054513628407 10.127531882970743 21.10527631907977 2 28.625 27.775 26.424999999999997 17.175 3 23.45 27.85 30.075000000000003 18.625 4 25.424999999999997 32.324999999999996 23.225 19.025 5 27.85 35.425000000000004 20.1 16.625 6 23.925 38.625 21.25 16.2 7 24.05 21.775 36.75 17.424999999999997 8 24.0 24.75 28.299999999999997 22.95 9 25.3 24.275 28.9 21.525 10-14 25.869999999999997 28.765 25.52 19.845 15-19 26.02 27.089999999999996 26.57 20.32 20-24 26.095000000000002 29.28 24.905 19.72 25-29 25.595000000000002 29.445 25.095 19.865 30-34 25.34 27.875 27.150000000000002 19.634999999999998 35-39 25.245 29.330000000000002 25.28 20.145 40-44 25.7 28.860000000000003 26.119999999999997 19.32 45-49 25.445 27.939999999999998 27.084999999999997 19.53 50-54 23.03 29.115000000000002 27.815 20.04 55-59 24.884999999999998 28.01 27.125 19.98 60-64 25.540000000000003 28.46 27.055 18.945 65-69 25.319999999999997 28.810000000000002 26.205000000000002 19.665 70-74 24.57 29.310000000000002 26.69 19.43 75-79 24.035 29.18 27.445000000000004 19.34 80-84 23.76 28.475 28.125 19.64 85-89 24.395 28.005000000000003 27.075 20.525 90-94 25.39 26.865 27.525 20.22 95-99 24.635 30.055 26.005 19.305 100-104 24.725 28.58 27.735 18.96 105-109 24.705 28.485 27.52 19.29 110-114 24.709999999999997 28.804999999999996 27.55 18.935 115-119 25.995 28.04 26.845000000000002 19.12 120-124 25.85 28.470000000000002 26.490000000000002 19.189999999999998 125-129 25.645 29.085 26.405 18.865000000000002 130-134 27.250000000000004 28.794999999999998 26.384999999999998 17.57 135-139 26.541327066353315 28.33641682084104 26.86134306715336 18.260913045652284 140-144 28.05420813121968 29.734460169025358 25.11376706505976 17.097564634695203 145-149 27.741935483870968 28.217054263565895 26.046511627906977 17.994498624656163 150-151 27.51593949243655 29.216152019002372 25.740717589698715 17.527190898862358 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.5 2 0.5 3 0.0 4 0.5 5 1.0 6 0.5 7 1.5 8 1.5 9 1.5 10 3.0 11 1.5 12 0.5 13 1.0 14 1.5 15 2.0 16 2.0 17 2.0 18 2.5 19 1.5 20 0.0 21 1.5 22 2.0 23 1.0 24 1.5 25 2.5 26 6.0 27 6.5 28 3.0 29 5.0 30 8.0 31 18.0 32 24.5 33 27.0 34 44.0 35 51.5 36 63.5 37 87.5 38 126.0 39 151.5 40 166.5 41 221.0 42 242.5 43 274.0 44 318.5 45 295.0 46 272.5 47 273.0 48 253.5 49 217.5 50 179.5 51 139.0 52 97.5 53 71.5 54 58.5 55 47.5 56 38.0 57 23.0 58 15.0 59 13.0 60 13.0 61 17.0 62 10.5 63 1.0 64 2.5 65 2.0 66 1.5 67 2.0 68 2.0 69 1.5 70 0.5 71 1.0 72 0.5 73 1.0 74 1.0 75 0.5 76 0.5 77 0.0 78 0.5 79 2.5 80 3.0 81 2.0 82 4.5 83 5.5 84 6.5 85 5.5 86 1.5 87 3.0 88 5.5 89 4.5 90 2.5 91 1.0 92 1.0 93 2.0 94 3.0 95 2.0 96 0.0 97 0.0 98 0.0 99 0.5 100 7.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.025 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.005 140-144 0.015 145-149 0.025 150-151 0.0125 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 59.75 #Duplication Level Percentage of deduplicated Percentage of total 1 65.18828451882845 38.95 2 19.07949790794979 22.8 3 8.493723849372385 15.225 4 3.6401673640167367 8.7 5 1.6317991631799162 4.875 6 1.00418410041841 3.5999999999999996 7 0.37656903765690375 1.575 8 0.16736401673640167 0.8 9 0.08368200836820083 0.44999999999999996 >10 0.33472803347280333 3.025 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGT 28 0.7000000000000001 No Hit GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG 20 0.5 No Hit GATTGATTTGGCATCCGAGCATGAAAGATACCTGACAGAGGAGATATTTA 15 0.375 No Hit GAGAAGTTACCTGGGTACCACCCCAAGACTGAAGAAGAGAAGGAGAAAGA 13 0.325 No Hit GTGCTTCTGTAGCTTTGACATCTCTGTCAAATGAAAAGCTCTTCGTGGTG 12 0.3 No Hit CCAGGACTCTGACAAATGTGGGTGTTCCAGCAACATACAAGCTTTCCATG 12 0.3 No Hit CAGGCAGTGTGCACCCCCTAAAGAAGAAAAGAGCTCTGATCCTGAGATAT 11 0.27499999999999997 No Hit GTTTCAGCCTCTGCTCCTCCCCAGGCCATGCCTTCATCTGTTCCTGTTCT 10 0.25 No Hit GTGAGATTCATCAATGAAAAGAACATCCATTTAGTCTGTGATGAGATTTA 9 0.22499999999999998 No Hit GATTATTAGCCAAGGTCTTCGCCTTCGCATGGCTGAATGCCTGATTGCTT 9 0.22499999999999998 No Hit GCAGATCGCATGGTTTACATATGGGACACAACTTCTCGACGCATCTTGTA 8 0.2 No Hit GCTAGTGGCGGTAGTTTGGGTCGTTGGCTTCGACATTTGCAACAAGCAGG 8 0.2 No Hit GCCTGATCTATCATGGGTTCAATCACTTGTCAAGGAATCTCCAACTGAGA 8 0.2 No Hit GTTAGAACTGGAACTTACAAAAACAGGGAGGTACGTAGGAGGGTTGGAGG 8 0.2 No Hit GAAGAGATTGAGAAGTTAATAAAAGTGAAGGCTCAAGTTTCAGAAGTTAA 7 0.17500000000000002 No Hit GTCTGTGTGGTGGACAGGCACTTACAGTATGGTACTCTCAAAAGCAGCTT 7 0.17500000000000002 No Hit GTTCAAGGCTATGGATACTGATAACAGTGGTGCAATCACATTTGATGAAC 7 0.17500000000000002 No Hit GGTGGTGATTGATAGGCTCACTGGACGCACAAAGGGTTATGGATTTGTTC 7 0.17500000000000002 No Hit CCCAATGTCACATTCTTGAAGGTGGATGTGGATGAATTGAAGGCTGTTGC 7 0.17500000000000002 No Hit CCCCTAAGCTGGCTCGTGCCAAGTGAAATATTTCCTATGAAAATTCGATC 7 0.17500000000000002 No Hit AAGTGGTTTGCGTGGCTTCAAATCTGAAGCACTTACTGAGGGTTCCAGCA 7 0.17500000000000002 No Hit TCCTACTTCATGCAGCGCCGGTCCTGTTGCTGAAGACATGTTCCATTGGC 7 0.17500000000000002 No Hit GCTCAAGGCCAGAATACAGGAAACTTCATTACTGATCGCCCATCCACTAA 7 0.17500000000000002 No Hit CATGACTTTGACCTTAATGTTCCAGCCTTGCCAGAGTTCTCTTCAGATTT 6 0.15 No Hit AGAAAACAATGGCACTACATGGAAAGATTGAGACAACATTAGAACTCAAG 6 0.15 No Hit GATACTATGTCGCCATCCTTTTAATGAATCCAAGAGAGCGTATGTGGTTC 6 0.15 No Hit CTAAAAACATGTCTCTAAAGGGTGAGCTGGAGACTGTCATAGAACTCAAA 6 0.15 No Hit CAACCCTAAATTCCCAATGAAACTCCCAAAACACCCCCCGTGGAAAAAAT 6 0.15 No Hit GTCCTTGGATCGATCTGAGAGGTAGAAGCTCCATTGCGCAATCATGTTGG 6 0.15 No Hit GCACAATAACAATTCTTTGTATTTGTATCTTTACAAAGCAATTGTATTTT 6 0.15 No Hit GTGAAGTCTAAGAAGGAGAAGCTCGACAGGAAGAGGAAGCCTGTCTCAAA 6 0.15 No Hit GCTCATGAAGGTCTTGAAAGAACTGGGTTCTCTGCCGGCAAATCTATCCT 6 0.15 No Hit TCTTGATCTGGTATGTCGAGCGCACCAACTTGTACAAGAAGGTCTCAAGT 6 0.15 No Hit CCAGATGTTGCAATGGAGATGATTGATGATGATACAGATTGTATTATCTT 6 0.15 No Hit GAGATGCCTGCAGCAATTGAAACAGGATTACTATCTTGTGAAGAGCAAGC 6 0.15 No Hit AGCTAACAGGCTTCCTGCTCAATTCCTAACTAACTAGAGCAGGCACTGCT 6 0.15 No Hit ATGATGACCATGATTCATTCAAAGTTGTTGATGATAAGGAGATTAAGGAA 6 0.15 No Hit GTTTTGACCGATGTAATATGATTCGAAGGCCGTTTCAAAGTTTCAGAGAA 6 0.15 No Hit GTGTCACTCGCCTGCCTCGGAAGACCCACAGGGGTCTCCGAAAGGTGGCT 6 0.15 No Hit GTAGCTTCTAAATTCAAAAGGAGCTATTAGCTTTTGATAGAAATGGCTTC 6 0.15 No Hit CTTCTTTGTCAGTAGGGTGGACACTCTCATTGACAAAATGCTAGAAAAGA 6 0.15 No Hit GGCTGATATTACAACAGTTGAAGATTTTCTCCGTGTTCTTTTTAGGGATT 6 0.15 No Hit GGCAAAACTATGTCAAGACATGGTTTAACCAACCAGCTCGAAAGACCCGC 6 0.15 No Hit ATCTGAGGTATCTTCTGTCATTTATAAAGTTAGTGAATGCATGCAAGAGC 6 0.15 No Hit GTGCTTATCTGTCGAATCTACATCTTTCTGAGCAATGGGGTTTGTTAGGT 6 0.15 No Hit GGGGCAACTGGGAATCATGTCCTGATATCTTTGACCAAGTATCCCCTGAT 6 0.15 No Hit GCTGAGGAGTGGAATGTGGAGGCAATGCCAACTTTTATTTTCCTGAAAGA 6 0.15 No Hit CTGGAACACATAAAACCCTTAATCAACCCGAAACCCTGCTGCCGCTGCTG 5 0.125 No Hit GGGGTCTATGGTAAGGGTGCGTATCCTGGTTATGCTGGGGATTTGTTAGT 5 0.125 No Hit GTGCAAGGGAGGAATGTACTGACAAACTTTTGGGGAATGAGCTTCACAAC 5 0.125 No Hit GGGAAATTCACAAAATGGAGCAGCTGGGAATGAGCAGAGAAAAGGGTCTA 5 0.125 No Hit ATTGACAAGTAACTTCCACGCCGTTTCCTGGAAGGTTAGACAGGAGAAAA 5 0.125 No Hit AAGGCATTTGGCACTGATGGTGCTCTGAAGTCTCACTCACAAGCCAAGCA 5 0.125 No Hit TGGAGATCTGCCAATAGCTAGAAGGAAATCACTGCAGAGGTTTTTGGAGA 5 0.125 No Hit GCTGACCACTAGCAATGCAGGAAGGATCACTAACCAATAAAAAAGAAAAA 5 0.125 No Hit GCTGTCAAGATAACAGAGAAGATAGAAGTTGCATCAACCAAGCAGCTTAA 5 0.125 No Hit TGCAAATGAGAAAACGTCGCCGCATGGCTCCAACCAAGCAGTTTTATGGA 5 0.125 No Hit CACAAAAAAGAAGGGAGAAAGAGGCTTTATCGAGGGATGCCTCTTCGCTC 5 0.125 No Hit GCTATAATTTGCCAGTCTTGTAAGACGTTCTTGGCAGGAGCATACTTCAC 5 0.125 No Hit GTTCTAGTTAACATTGAGCAACAAAGTCCTGATATTGCTCAGGGTGTACA 5 0.125 No Hit AAAGAAGTTACTAGGATCAGATTCCCGTTTACGCCCTGATAGATATGCTC 5 0.125 No Hit TTCTAAACCCCTCCCCCTTTTTGCTTCTTAATTTCTCACATTTTGTCCCA 5 0.125 No Hit GTTGCTGTTGGGGTTCTTGTGGGGTGGGCATGGAAGCCTAAATGGGCTAG 5 0.125 No Hit TTTGAAGAGGGAGAAAGTTGTCTTGGCTGGTCTAGGAGTGGAGATGTATG 5 0.125 No Hit AATTGGCTGCCATTAATTTGGAGTCCAGAGCTGCCTTACTTGTGGCAAAG 5 0.125 No Hit CGCCTAATAGGAGAGGCCAATGATTACGTGGGAAAGGGTATGGCTGGAGG 5 0.125 No Hit AGTCAACCTTGGCCATATTTGAATGAAGCTTTGACCAAGTTCCAAGATCC 5 0.125 No Hit GCCCTATAGTGGTCATTATCTCCCTAACAAGGACAATTTCAAAGAGTTCA 5 0.125 No Hit AGGGAATGGATGTTGTACAGGCTATTGAGAAGGTGGACAAAGCAGACAAG 5 0.125 No Hit ATACATCAGAGATGGACTCAGTATTGAGTTCAGATCACCGAGCTCCCACA 5 0.125 No Hit GATTGATACTGCACTATTGTACAAACATCCCCGGGGTGGAAATTATAAAA 5 0.125 No Hit GCGACCGAGATCCGGATGCGCAGGTCCAAGGAGGCGTCCCTGACGGTTTC 5 0.125 No Hit TATGTGAAGGATTATTTTGGTTTCGACCATGATTTTCTTGGAGTAGTCGC 5 0.125 No Hit GTTTCTATTTTCCTCTGTTTACAGTTTACACCTTTCTTTATATAGAAAGA 5 0.125 No Hit AACAAGGCAAGACCTTATGAAATGCCATGCATCTGAAACACAAGACTGGA 5 0.125 No Hit CCGTGACCGTGGTTATGGTGACGGTGGATCTAGGTACTCTTCAAGGGGTG 5 0.125 No Hit TGATGTTCGAGTTACTGTAGCTGCACCTGCTGCCCGTGGAGAAGCTAACA 5 0.125 No Hit GCCCGTGTTTGGTGGAGGCATGCCTCAGACCTATATCTATCCGATTCACC 5 0.125 No Hit TATCAAATTAAAGCCTGCCATCTACCCTTATCAATGCCAGCATCTCTCTT 5 0.125 No Hit GTGAGTTTTTGGGGTTGCATGGTGGTGATGGCTGCCAAGAAAGCGTGTGA 5 0.125 No Hit GATCTATGTGAGTGAGAAGGGAACTGTAGTTGAGGAAGAGTGAGTCAAGG 5 0.125 No Hit AATGAGAAGATTAATTCCCGAAGAAAATGACCCCGAGTATGCAAATTTCC 5 0.125 No Hit ATTCACGTAATTTACGTCGAGTCAGGCAGGGTCTTGACCTTATCAGGGCT 5 0.125 No Hit AGCATGTTAAGGCAAAGCACCCATTGGCACAACCTCGAGCTGTGGATCCG 5 0.125 No Hit GAAGCGAACCAAGGTGCTTAACATCCTTAAAAAGGGACTGCAAGTTGCAG 5 0.125 No Hit ATTGAACAGGCATTAGGGAAGCATGGCATTGTATGTCTAGAAGATATTGT 5 0.125 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.025 0.0 0.0 0.0 0.0 52-53 0.05 0.0 0.0 0.0 0.0 54-55 0.05 0.0 0.0 0.0 0.0 56-57 0.075 0.0 0.0 0.0 0.0 58-59 0.075 0.0 0.0 0.0 0.0 60-61 0.075 0.0 0.0 0.0 0.0 62-63 0.075 0.0 0.0 0.0 0.0 64-65 0.075 0.0 0.0 0.0 0.0 66-67 0.075 0.0 0.0 0.0 0.0 68-69 0.11249999999999999 0.0 0.0 0.0 0.0 70-71 0.15 0.0 0.0 0.0 0.0 72-73 0.15 0.0 0.0 0.0 0.0 74-75 0.15 0.0 0.0 0.0 0.0 76-77 0.1875 0.0 0.0 0.0 0.0 78-79 0.2375 0.0 0.0 0.0 0.0 80-81 0.25 0.0 0.0 0.0 0.0 82-83 0.275 0.0 0.0 0.0 0.0 84-85 0.3125 0.0 0.0 0.0 0.0 86-87 0.375 0.0 0.0 0.0 0.0 88-89 0.525 0.0 0.0 0.0 0.0 90-91 0.5875 0.0 0.0 0.0 0.0 92-93 0.7125 0.0 0.0 0.0 0.0 94-95 0.8125 0.0 0.0 0.0 0.0 96-97 0.875 0.0 0.0 0.0 0.0 98-99 1.1625 0.0 0.0 0.0 0.0 100-101 1.4375 0.0 0.0 0.0 0.0 102-103 1.8250000000000002 0.0 0.0 0.0 0.0 104-105 2.175 0.0 0.0 0.0 0.0 106-107 2.825 0.0 0.0 0.0 0.0 108-109 3.45 0.0 0.0 0.0 0.0 110-111 3.925 0.0 0.0 0.0 0.0 112-113 4.5 0.0 0.0 0.0 0.0 114-115 5.0125 0.0 0.0 0.0 0.0 116-117 6.025 0.0 0.0 0.0 0.0 118-119 6.7625 0.0 0.0 0.0 0.0 120-121 7.7375 0.0 0.0 0.0 0.0 122-123 8.4125 0.0 0.0 0.0 0.0 124-125 9.3625 0.0 0.0 0.0 0.0 126-127 10.1125 0.0 0.0 0.0 0.0 128-129 11.175 0.0 0.0 0.0 0.0 130-131 12.1 0.0 0.0 0.0 0.0 132-133 13.4 0.0 0.0 0.0 0.0 134-135 14.675 0.0 0.0 0.0 0.0 136-137 15.8 0.0 0.0 0.0 0.0 138-139 16.9375 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTGATC 10 0.006830828 145.0 1 TGGCATC 10 0.006830828 145.0 9 ATCTTAC 10 0.006830828 145.0 5 TACAGGC 10 0.006830828 145.0 9 GATTGAT 10 0.006830828 145.0 1 GATCTTA 10 0.006830828 145.0 4 CTTACAG 10 0.006830828 145.0 7 ATTGATT 10 0.006830828 145.0 2 GTGATCT 10 0.006830828 145.0 2 >>END_MODULE Read 794379 spots for SRR26075380.sra Written 794379 spots for SRR26075380.sra Read 794379 spots for SRR26075380.sra Written 794379 spots for SRR26075380.sra Read 794379 spots for SRR26075380.sra Written 794379 spots for SRR26075380.sra Read 794379 spots for SRR26075380.sra Written 794379 spots for SRR26075380.sra Read 794379 spots for SRR26075380.sra Written 794379 spots for SRR26075380.sra Read 794379 spots for SRR26075380.sra Written 794379 spots for SRR26075380.sra Read 794379 spots for SRR26075380.sra Written 794379 spots for SRR26075380.sra Read 794379 spots for SRR26075380.sra Written 794379 spots for SRR26075380.sra Read 794379 spots for SRR26075380.sra Written 794379 spots for SRR26075380.sra Read 794379 spots for SRR26075380.sra Written 794379 spots for SRR26075380.sra Read 794379 spots for SRR26075380.sra Written 794379 spots for SRR26075380.sra Read 794393 spots for SRR26075380.sra Written 794393 spots for SRR26075380.sra Read 794379 spots for SRR26075380.sra Written 794379 spots for SRR26075380.sra Read 794379 spots for SRR26075380.sra Written 794379 spots for SRR26075380.sra Read 794379 spots for SRR26075380.sra Written 794379 spots for SRR26075380.sra Read 794379 spots for SRR26075380.sra Written 794379 spots for SRR26075380.sra Read 794379 spots for SRR26075380.sra Written 794379 spots for SRR26075380.sra Read 794379 spots for SRR26075380.sra Written 794379 spots for SRR26075380.sra Read 794379 spots for SRR26075380.sra Written 794379 spots for SRR26075380.sra Read 794379 spots for SRR26075380.sra Written 794379 spots for SRR26075380.sra SRR ids: ['SRR26075380.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_0ebmj5c3 SRR26075380.sra spots: 15887594 blocks: [[1, 794379], [794380, 1588758], [1588759, 2383137], [2383138, 3177516], [3177517, 3971895], [3971896, 4766274], [4766275, 5560653], [5560654, 6355032], [6355033, 7149411], [7149412, 7943790], [7943791, 8738169], [8738170, 9532548], [9532549, 10326927], [10326928, 11121306], [11121307, 11915685], [11915686, 12710064], [12710065, 13504443], [13504444, 14298822], [14298823, 15093201], [15093202, 15887594]] SRR26075380 file size 5861128 SRR26075380 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o SRR26075380 SRR26075380_1.fastq SRR26075380_2.fastq Input file: SRR26075380_1.fastq Paired file: SRR26075380_2.fastq trimmed: SRR26075380-trimmed-pair1.fastq, SRR26075380-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Tue Feb 11 23:40:40 2025 >> started Tue Feb 11 23:40:58 2025 >> done (17.947s) 15887594 read pairs processed; of these: 122 ( 0.00%) short read pairs filtered out after trimming by size control 165639 ( 1.04%) empty read pairs filtered out after trimming by size control 15721833 (98.96%) read pairs available; of these: 3997831 (25.43%) trimmed read pairs available after processing 11724002 (74.57%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 18 5 0.00% 19 8 0.00% 20 10 0.00% 21 8 0.00% 22 10 0.00% 23 17 0.00% 24 30 0.00% 25 21 0.00% 26 26 0.00% 27 34 0.00% 28 33 0.00% 29 33 0.00% 30 27 0.00% 31 42 0.00% 32 35 0.00% 33 21 0.00% 34 43 0.00% 35 22 0.00% 36 44 0.00% 37 54 0.00% 38 82 0.00% 39 71 0.00% 40 49 0.00% 41 59 0.00% 42 71 0.00% 43 80 0.00% 44 105 0.00% 45 61 0.00% 46 98 0.00% 47 95 0.00% 48 135 0.00% 49 126 0.00% 50 149 0.00% 51 154 0.00% 52 178 0.00% 53 211 0.00% 54 232 0.00% 55 226 0.00% 56 246 0.00% 57 265 0.00% 58 290 0.00% 59 253 0.00% 60 360 0.00% 61 354 0.00% 62 455 0.00% 63 525 0.00% 64 585 0.00% 65 583 0.00% 66 612 0.00% 67 779 0.00% 68 857 0.01% 69 924 0.01% 70 1025 0.01% 71 1184 0.01% 72 1308 0.01% 73 1542 0.01% 74 1822 0.01% 75 2048 0.01% 76 2286 0.01% 77 2706 0.02% 78 2856 0.02% 79 3230 0.02% 80 3583 0.02% 81 4164 0.03% 82 4845 0.03% 83 5259 0.03% 84 6035 0.04% 85 6830 0.04% 86 7377 0.05% 87 8194 0.05% 88 8777 0.06% 89 9866 0.06% 90 10917 0.07% 91 12263 0.08% 92 13453 0.09% 93 15062 0.10% 94 16004 0.10% 95 17357 0.11% 96 18661 0.12% 97 20176 0.13% 98 21396 0.14% 99 22588 0.14% 100 24197 0.15% 101 25597 0.16% 102 27731 0.18% 103 29348 0.19% 104 31704 0.20% 105 33395 0.21% 106 36394 0.23% 107 37348 0.24% 108 39117 0.25% 109 41215 0.26% 110 41759 0.27% 111 44560 0.28% 112 46680 0.30% 113 48713 0.31% 114 50991 0.32% 115 53885 0.34% 116 55336 0.35% 117 58399 0.37% 118 59376 0.38% 119 62644 0.40% 120 63585 0.40% 121 65405 0.42% 122 66681 0.42% 123 69989 0.45% 124 72537 0.46% 125 74566 0.47% 126 78002 0.50% 127 80951 0.51% 128 82085 0.52% 129 83570 0.53% 130 84688 0.54% 131 86348 0.55% 132 87050 0.55% 133 90742 0.58% 134 91303 0.58% 135 95367 0.61% 136 96977 0.62% 137 98510 0.63% 138 99979 0.64% 139 101697 0.65% 140 102368 0.65% 141 103850 0.66% 142 105694 0.67% 143 108169 0.69% 144 110065 0.70% 145 112320 0.71% 146 113049 0.72% 147 112368 0.71% 148 115342 0.73% 149 115825 0.74% 150 117753 0.75% 151 11724002 74.57% 15721833 reads passed initial QC criterion=sequence-density sequence-density=0.28 sequence-density-rank=1 fanout-score=2.69 fanout-score-rank=41 prefix-density=0.29 prefix-fanout=2.6 sequence=GTGGACTCCTTCTGGAT criterion=fanout-score sequence-density=0.01 sequence-density-rank=42 fanout-score=46.76 fanout-score-rank=1 prefix-density=0.05 prefix-fanout=6.3 sequence=AAAAGAATTAAAGAATTGCACCAATTGATTAATTGCCTAAATTACAAAAACTAATTGAATATCTTATGTGGCAAAACTTGGACAGGTAATGCAAGGACGTCTTCTTTCTAGGACATGCTG criterion=sequence-density sequence-density=0.32 sequence-density-rank=1 fanout-score=3.25 fanout-score-rank=23 prefix-density=0.39 prefix-fanout=2.7 sequence=ATCCAGAAGGAGTCCAC criterion=fanout-score sequence-density=0.11 sequence-density-rank=18 fanout-score=117.19 fanout-score-rank=1 prefix-density=0.77 prefix-fanout=16.9 sequence=ATGATGATGCTTGATGTGTGGCTTGTTTGGTTATGTCCATCTACTGTTTTTCTTCCTTTTTAGAAAAAAAAGCTCGGTTTACTGCAAATATTACAAGACCATACGTGCTTGTAATTTGA SRR26075380 testing PE reads STAR mapping to Ensembl genome Started job on | Feb 11 23:41:46 Started mapping on | Feb 11 23:41:46 Finished on | Feb 11 23:43:49 Mapping speed, Million of reads per hour | 460.15 Number of input reads | 15721833 Average input read length | 290 UNIQUE READS: Uniquely mapped reads number | 14155547 Uniquely mapped reads % | 90.04% Average mapped length | 288.57 Number of splices: Total | 9738134 Number of splices: Annotated (sjdb) | 9462262 Number of splices: GT/AG | 9557250 Number of splices: GC/AG | 124573 Number of splices: AT/AC | 10779 Number of splices: Non-canonical | 45532 Mismatch rate per base, % | 0.40% Deletion rate per base | 0.04% Deletion average length | 3.00 Insertion rate per base | 0.03% Insertion average length | 2.40 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 532492 % of reads mapped to multiple loci | 3.39% Number of reads mapped to too many loci | 67986 % of reads mapped to too many loci | 0.43% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 5.64% % of reads unmapped: other | 0.50% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 1033794 1033794 1033794 N_multimapping 532492 532492 532492 N_noFeature 447986 13978693 544696 N_ambiguous 172797 1057 92220 UnstrandedReadsAssigned:13534764 PositiveStrandReadsAssigned:175797 NegativeStrandReadsAssigned:13518631 Dataset is classified negative stranded MeadianReadLen=151 20thPercentileLength=142 echo kmer=137 SRR26075380 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,400 [index] number of k-mers: 62,057,036 [index] number of equivalence classes: 130,681 [quant] running in paired-end mode [quant] will process pair 1: SRR26075380-trimmed-pair1.fastq SRR26075380-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 15,721,833 reads, 13,898,721 reads pseudoaligned [quant] estimated average fragment length: 180.15 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,267 rounds 52401 SRR26075380.ke.tsv 34699 SRR26075380.se.tsv 87100 total ==> SRR26075380.ke.tsv <== target_id length eff_length est_counts tpm Potri.005G200100.1.v4.1 2018 1838.85 2657 76.0762 Potri.005G024800.1.v4.1 1035 855.85 6929 426.262 Potri.004G059700.1.v4.1 961 781.856 25 1.68351 Potri.007G009000.2.v4.1 1416 1236.85 0 0 Potri.003G141000.2.v4.1 2943 2763.85 460 8.76288 Potri.016G087400.1.v4.1 270 98.4902 2123.89 1135.38 Potri.015G069301.1.v4.1 564 384.938 0 0 Potri.010G195200.1.v4.1 1773 1593.85 44 1.45348 Potri.012G127500.1.v4.1 977 797.856 3101 204.635 ==> SRR26075380.se.tsv <== Potri.001G166300.v4.1 0 Potri.001G448400.v4.1 65 Potri.001G233950.v4.1 0 Potri.001G122700.v4.1 133 Potri.001G212900.v4.1 0 Potri.001G182400.v4.1 0 Potri.001G256600.v4.1 0 Potri.001G040500.v4.1 28 Potri.001G416900.v4.1 0 Potri.001G452600.v4.1 420 SRR26075380 completed mapping pipeline successfully