Starting /dee2/code/volunteer_pipeline.sh SRR26075381 current disk space = 3052129652736 free memory = 1478251168 SRR26075381 SRAfilesize ed518d8613c32b50cf1406ea5927f7b4 SRR26075381.sra SRR26075381.sra file validated SRR26075381 is paired end SRR26075381 is conventional basespace SRR26075381 read1 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR26075381_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.63625 37.0 37.0 37.0 37.0 37.0 2 36.6015 37.0 37.0 37.0 37.0 37.0 3 36.7165 37.0 37.0 37.0 37.0 37.0 4 36.7155 37.0 37.0 37.0 37.0 37.0 5 36.6975 37.0 37.0 37.0 37.0 37.0 6 36.7385 37.0 37.0 37.0 37.0 37.0 7 36.705 37.0 37.0 37.0 37.0 37.0 8 36.6745 37.0 37.0 37.0 37.0 37.0 9 36.6585 37.0 37.0 37.0 37.0 37.0 10-14 36.669 37.0 37.0 37.0 37.0 37.0 15-19 36.606 37.0 37.0 37.0 37.0 37.0 20-24 36.561499999999995 37.0 37.0 37.0 37.0 37.0 25-29 36.5456 37.0 37.0 37.0 37.0 37.0 30-34 36.4365 37.0 37.0 37.0 37.0 37.0 35-39 36.40880000000001 37.0 37.0 37.0 37.0 37.0 40-44 36.3799 37.0 37.0 37.0 37.0 37.0 45-49 36.319100000000006 37.0 37.0 37.0 37.0 37.0 50-54 36.2688 37.0 37.0 37.0 37.0 37.0 55-59 36.137 37.0 37.0 37.0 37.0 37.0 60-64 36.0903 37.0 37.0 37.0 37.0 37.0 65-69 36.0802 37.0 37.0 37.0 37.0 37.0 70-74 36.086600000000004 37.0 37.0 37.0 37.0 37.0 75-79 36.1231 37.0 37.0 37.0 37.0 37.0 80-84 36.0711 37.0 37.0 37.0 37.0 37.0 85-89 35.9206 37.0 37.0 37.0 37.0 37.0 90-94 35.880700000000004 37.0 37.0 37.0 37.0 37.0 95-99 35.8877 37.0 37.0 37.0 37.0 37.0 100-104 35.842499999999994 37.0 37.0 37.0 37.0 37.0 105-109 35.744 37.0 37.0 37.0 37.0 37.0 110-114 35.6026 37.0 37.0 37.0 37.0 37.0 115-119 35.6103 37.0 37.0 37.0 37.0 37.0 120-124 35.597500000000004 37.0 37.0 37.0 37.0 37.0 125-129 35.357600000000005 37.0 37.0 37.0 37.0 37.0 130-134 35.310599999999994 37.0 37.0 37.0 34.6 37.0 135-139 35.2179 37.0 37.0 37.0 29.8 37.0 140-144 35.1115 37.0 37.0 37.0 27.4 37.0 145-149 35.0278 37.0 37.0 37.0 25.0 37.0 150-151 34.93 37.0 37.0 37.0 25.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.0 1101 2 0.0 1101 3 0.0 1101 4 0.0 1101 5 0.0 1101 6 0.0 1101 7 0.0 1101 8 0.0 1101 9 0.0 1101 10-14 0.0 1101 15-19 0.0 1101 20-24 0.0 1101 25-29 0.0 1101 30-34 0.0 1101 35-39 0.0 1101 40-44 0.0 1101 45-49 0.0 1101 50-54 0.0 1101 55-59 0.0 1101 60-64 0.0 1101 65-69 0.0 1101 70-74 0.0 1101 75-79 0.0 1101 80-84 0.0 1101 85-89 0.0 1101 90-94 0.0 1101 95-99 0.0 1101 100-104 0.0 1101 105-109 0.0 1101 110-114 0.0 1101 115-119 0.0 1101 120-124 0.0 1101 125-129 0.0 1101 130-134 0.0 1101 135-139 0.0 1101 140-144 0.0 1101 145-149 0.0 1101 150-151 0.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 18 1.0 19 1.0 20 2.0 21 1.0 22 4.0 23 1.0 24 3.0 25 5.0 26 7.0 27 14.0 28 33.0 29 28.0 30 27.0 31 50.0 32 55.0 33 95.0 34 148.0 35 407.0 36 2908.0 37 210.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 37.82227784730914 16.07008760951189 8.610763454317897 37.49687108886108 2 18.85 14.174999999999999 34.375 32.6 3 15.525 17.849999999999998 28.349999999999998 38.275 4 20.95 23.925 24.224999999999998 30.9 5 24.2 26.125 25.35 24.325 6 23.0 31.424999999999997 24.575 21.0 7 13.775 31.900000000000002 38.75 15.575 8 17.424999999999997 27.675 31.55 23.35 9 18.975 26.200000000000003 31.924999999999997 22.900000000000002 10-14 18.66 31.0 27.76 22.58 15-19 18.86 28.615000000000002 27.74 24.785 20-24 20.02 28.4 28.4 23.18 25-29 19.095000000000002 28.749999999999996 28.685 23.47 30-34 19.189999999999998 29.744999999999997 27.36 23.705000000000002 35-39 20.044999999999998 28.17 27.450000000000003 24.335 40-44 19.5 29.38 27.560000000000002 23.56 45-49 20.580000000000002 27.55 28.215 23.655 50-54 20.330000000000002 27.694999999999997 27.605 24.37 55-59 18.825 28.685 28.035 24.455 60-64 20.375 28.754999999999995 27.41 23.46 65-69 20.9 28.115000000000002 26.69 24.295 70-74 20.62 28.975 26.83 23.575 75-79 19.31 28.42 27.169999999999998 25.1 80-84 20.965 27.605 28.24 23.189999999999998 85-89 21.09 27.815 27.325 23.77 90-94 20.025000000000002 28.910000000000004 26.395000000000003 24.67 95-99 20.78 27.839999999999996 27.315 24.065 100-104 21.035 27.700000000000003 27.66 23.605 105-109 21.224999999999998 27.700000000000003 27.205000000000002 23.87 110-114 20.325 27.51 27.800000000000004 24.365000000000002 115-119 21.425 27.965 27.435 23.175 120-124 21.34 28.175 26.745 23.74 125-129 22.009999999999998 26.790000000000003 27.83 23.369999999999997 130-134 21.095 28.225 26.86 23.82 135-139 21.11 28.04 26.590000000000003 24.26 140-144 21.505 27.284999999999997 27.015 24.195 145-149 21.295 27.095000000000002 27.005000000000003 24.605 150-151 21.4875 27.224999999999998 25.2 26.087500000000002 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.5 11 1.5 12 1.0 13 1.0 14 1.5 15 0.5 16 0.5 17 0.5 18 0.0 19 0.0 20 1.0 21 3.0 22 2.5 23 1.0 24 2.0 25 4.5 26 7.0 27 10.0 28 18.5 29 21.5 30 23.5 31 29.0 32 22.0 33 23.5 34 43.5 35 71.5 36 107.0 37 126.5 38 132.5 39 141.0 40 171.0 41 215.0 42 234.0 43 237.5 44 243.5 45 268.0 46 258.5 47 214.5 48 228.5 49 210.0 50 166.5 51 173.0 52 143.0 53 104.0 54 72.5 55 57.0 56 55.5 57 39.0 58 31.0 59 19.5 60 7.5 61 7.0 62 7.0 63 4.0 64 3.5 65 7.0 66 11.0 67 7.0 68 1.5 69 0.0 70 1.0 71 1.0 72 0.5 73 0.5 74 1.0 75 1.0 76 0.0 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.125 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150-151 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 57.4 #Duplication Level Percentage of deduplicated Percentage of total 1 59.059233449477354 33.900000000000006 2 23.562717770034844 27.05 3 9.36411149825784 16.125 4 4.2682926829268295 9.8 5 1.9163763066202089 5.5 6 0.9581881533101044 3.3000000000000003 7 0.39198606271777003 1.575 8 0.3048780487804878 1.4000000000000001 9 0.0 0.0 >10 0.17421602787456447 1.35 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGCCTGTATCTCGTAT 21 0.525 TruSeq Adapter, Index 27 (97% over 39bp) ATCACAATGATAAAAACCACACGTAGAAAGGAAAAACATGAAAACCAGGC 12 0.3 No Hit ATCTATTTCTGATACCAGCAAACTTCACATCCTGCAATACGTAGAGTGGC 11 0.27499999999999997 No Hit TTGGCGATAATGGGATTGCAGATGCTCTCGAGCTCTTTCATCTTGTCCTC 10 0.25 No Hit CTTCAATTTAACAACCAGCCTCCAATCTTCAATTGACAGATCATCCCGGT 8 0.2 No Hit ACCACAACTTGAAAGACAGATGATTAACAGAAAGGGATTCAGAGAAGCGG 8 0.2 No Hit CCACACAAACAATTTTAGCCACAAAAAATCCCAACAATTGCAAAGCCCTA 8 0.2 No Hit AAACCACCATCCTCCAACTTGCTCCAGCTTGTTTGGCAGTAGCTGATTCT 8 0.2 No Hit ACAGGTCCAAAAATAACAGCGTTCCAGAGCATGATGTTGTTGTCTTGAGG 8 0.2 No Hit GCTTCAACAGTGACAACACTATTGCCTCTGATCACCACCATGCCTATGTC 8 0.2 No Hit GACGGAAGGAATGGATTGTAAGATCTTCGAGATATTTTGGAAGACCGAGA 8 0.2 No Hit GGCCGGAATACACTCCTGCTGCGAGTCCCCATTGTAAGGATTCCTTTCCA 7 0.17500000000000002 No Hit CTTCTTACCAGCACTACTAAATATAGGTTCTTCAGTATCTAATGATTTCC 7 0.17500000000000002 No Hit CTCCCGAGAAGATTCACCCAACCAGACCTAAACCCCAAGCTTGTGCTCTA 7 0.17500000000000002 No Hit GCCGTGCTTGCCTAGACCCTGCTCAATGATCGAGTTGTCAGTCAAAGGAA 7 0.17500000000000002 No Hit CTCATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGT 7 0.17500000000000002 No Hit TGCGTTTGATACATTCCCATTTTCACTACAAACAAAGCATAGATTGCAGG 7 0.17500000000000002 No Hit GCAGCTACAAAGAAGTGGGCTAAGATTGCAGCTACAGAGGCAACAGGTTT 7 0.17500000000000002 No Hit AGCCATCATCTAAGAGATCAACTTCACTGGTTGTCTGCACAATGATTCTG 7 0.17500000000000002 No Hit CTTGTTGCTATCATATTTGCTTGTGCAGCCAAGATTTGTAGACACGGTTC 7 0.17500000000000002 No Hit CTCGGGATGACTTTGGGAAGTGAACCCGGGCCTCTTGGACGATTCCTAGG 6 0.15 No Hit GATGCTTGAGTGATAATTTGAGGATCACACTGATCAACTCCATCATTGGC 6 0.15 No Hit GCCCACACATGTATACTCTGTAAGTTGCAGCACAACCTAATTAATTAGGA 6 0.15 No Hit GAGGCCATCAAGATGAAGGAGATCATAAGTTCGAGGGTAAGTTGAGAAAG 6 0.15 No Hit CTTTGTTCCAGGTGAGCAACCTGCATAAAGTATCCGGCTAAAATAGATTT 6 0.15 No Hit GTCGCCTTCTTTTAACATACTCCTTAAATCATCATCACTCAAATCTTGCA 6 0.15 No Hit GTCTATTTTGTCTCCTTCGCTGCCGAACGAGGATTGAGCTGCCCCAGCCA 6 0.15 No Hit ATGGGAAAGAGGTTACTCCATACATGAGGAGGAAGCCCTACTGAACCCTC 6 0.15 No Hit CTTCTAATATCACCCCAGCCTCTATTCCACTGGTTGGCAAGAAGCCAATA 6 0.15 No Hit CCACCTCCCAGAATTTTGCACCAATTTGATTGCCGCATTGGCCTCCTTGG 6 0.15 No Hit GTTTATTGGGCTTACAAACTAGGCTTGCAAGGTGTTTAGTAAACCGTGAG 6 0.15 No Hit GCTCCATAATCACAACTTTTGAGCACCAAAAGGAACTTCTTTGCAAAGGA 6 0.15 No Hit ATCATCTCGGGACACATATCATTTATAATAATAATGAATCATTAGCTAAC 6 0.15 No Hit GCTAGTGACATTCAGTTTATTTGCCAGGAGAACAAGAAAAAAATATGAAG 6 0.15 No Hit GAACCGCTCTCGTGAATACTGCCTTGATGCTTTCCGCCAATCTGTGCCGG 6 0.15 No Hit CGTCGCCAGTGCCTGCGCTTCGCATTGTACCTGATGGTGTTATCAGTTCT 6 0.15 No Hit GCCACTGCGATCTGCATTAATTGATGTCATCAAGAACCACTCCAAGAGTT 6 0.15 No Hit CTTTGAAACAGCTTTTTCCCACAATAAAGTCTTCAAACCTGGTCGTATTG 6 0.15 No Hit CATTAAACCATAGTGTCTTGCTGATATCTAGCTCAAAAATATCATAATCC 6 0.15 No Hit GTCAGAGCCTAGAATTGGAAGGCGACCCTCACGCGCATAAGACTTCAAGG 6 0.15 No Hit GCTTGCTCCGCGCTAGTTCGATTAGCAAACTGAACGAATCCACAACGCTT 6 0.15 No Hit GCATCAATGCAGAATCGCCCTTGTTAACTGAATTTGAAGCCACAGAACAA 6 0.15 No Hit CCCGCTAAGAACACCTGGGTATAGAAATGCTACTGCTGAGATAAAGCATC 5 0.125 No Hit GGAAGAAAATGGACCAGAAGATGATGCTGCCAACGACATGGATCGGGTTA 5 0.125 No Hit GAAACAACAGCTACGGCATAAGCAAAATCTTCACTTGAGGGGAATAAACC 5 0.125 No Hit TTTGGTTCTTATGTAGAAATTTGAGAAAGTCGAGTAATTTCTGTTGCAAG 5 0.125 No Hit GCGAAGATGAGTCCTAACTGGTTCTGGTTTCTCACCGGGTGGAGCCTCCC 5 0.125 No Hit GGTTGGGTACTGCACCACGGTCAATGAAACTCTGGCTCCTCTTTGCAAAT 5 0.125 No Hit CCGGCAGAAACAGCAACACCACCACCGGATGGAACGGAAGCCAACTTTTC 5 0.125 No Hit CTAGAATTTTAGACATTTCTGCGGGATAATTTCCAAATATGATGGGGTCC 5 0.125 No Hit GGTCCATACAAAACTGCATCTGCTCCTTCAACTTCGGCCCAAATTCCTTC 5 0.125 No Hit CTACTGATTCAACAACGGTCTTGGAGAATCCCTTCTCAGCAAGCTTCAAA 5 0.125 No Hit CCCAAGCCACTTTCCATTTAAGTCATTAATTGCATTTTGAGCCTCCTGCT 5 0.125 No Hit AGTACAAGTAGCAGGGGTGGTTGCTGCTGCTGCTGACATTGCACTGTTAG 5 0.125 No Hit CCTGCTTCTTCTCCATATCGGTGTGAGACAAGTCCAGTTGTCACAATTGA 5 0.125 No Hit CTTCATTCACGGTGATGTTACGCCCATCAAGGTCTTGGCCGTTCATTCCA 5 0.125 No Hit CCTTAGTAGTCAAACTATCTGCAGTGACAGCATCCTTCAAATCCACATCC 5 0.125 No Hit CTCTTGTTTTTCATGCCACTTCTTTGCTTGTTTCTTCTTGTCACTTAAAA 5 0.125 No Hit AAGAGAACCAAATTGTGAATTCAGGTTTTCCAACCTCAAGCTTTTCCTTT 5 0.125 No Hit GACAGGATCGCAGCCCTCACCAATATATAAGAGAAGATATTTTCCATCAT 5 0.125 No Hit TCCAGCAAAAAAAAAAACAGCACTTCAATTGCTTAGACATCAGCCCAAAC 5 0.125 No Hit CTGAGAAACAAGCAGTGTATCTGGAGTGAGGAGGCTTTCACTATTCTCCT 5 0.125 No Hit ATTTCATCATCAAACTTTTTCTTCAGCTCAGGATGCTTCTCGAAGTACTC 5 0.125 No Hit GCTCGAGGGACTTATTGTAATTAGTGATGTAAGTCTGGTGGTGTTTCTGG 5 0.125 No Hit GGCGATTCATGAGATCAACATAGTACTTGTTGTCAAATTTATTAGGAGAT 5 0.125 No Hit ACCGCTCAAACGAAGTTGGAAAAAGATCCCCAGAAGCGGAGCCTCCAATC 5 0.125 No Hit GTCCTTCAACTTCCCACAGTATCCCATTCTCAATCTCCTTGTATGGGAAC 5 0.125 No Hit GTTCGGCGACGCTTGAAAGGGTGCTTTGGACATTTCCATATCTCTTCTTC 5 0.125 No Hit CCTTGATTCATTTAATTTTCAGCCTCTTTCCTATTCCCATAATTCATCTC 5 0.125 No Hit CTCTTTTCCTTCAAATTACCTGGCAAGCTCCAAGGATCAGTTTGGAATAC 5 0.125 No Hit GTTTTATTGATTGGAAGGATGTGGTAAAGAATGGGTGGTTTTGTTTGTTT 5 0.125 No Hit TCTTTATGTCACCCACAAGGCAAACATTTTTACATTAAGGAAAAGCAATA 5 0.125 No Hit CAGCTCTTTCTTTTCATCACTTGGCTTTCCATCCCATACCTGTTTCTTGA 5 0.125 No Hit GCTGGATAAGACCTTCTTTCTCTCCACTGAGGAGTTCCTTGAAATAGGAG 5 0.125 No Hit GGCACGTCAATCTAGAATGACGGTATGAACTCTTATCAAAATTATAATTT 5 0.125 No Hit CACAGTAAACGCAGCTAAAAGGAGCAGGGGTGTCGCGATACATAGTCTGT 5 0.125 No Hit GTCGGTTATCAAAAGAGAAGGCAACAGACAACACATCCTTGGTGTGGCCG 5 0.125 No Hit CCTGTTTCTTGAGCTGCTGTTGCTTAGCCTTTTTCTCCTGGATCATCTTC 5 0.125 No Hit GTATCGTCTCCTCACGTTATTCACTCCACCACGCCTGTATCTTCTAGATT 5 0.125 No Hit CTTCCGTCAACATGATGAACTGAACATCCAAAGGATTTTCAGAAACCCAT 5 0.125 No Hit AATCCGTTATTAAACCATGGAGAGATGGACCGTAAGCCGAATAGCTAGCC 5 0.125 No Hit GACAAGTTAGAGAAACAGCTCAGTTCTTTGGTGTGTTCTCCAATTCCTTT 5 0.125 No Hit CACCTAACGACAGATGTGCATAACCACAAAATGTCTTGACTGCTTCAGTA 5 0.125 No Hit GTCAAGATATCATCATCCAACTTGTGCAAAAATACTGTCGGGAAAATGTT 5 0.125 No Hit CTAGGCTTGATTCCCTTAAACTTTGTTAACTTGTAGACAAATGTCTTAGT 5 0.125 No Hit GCAACCTCTCTGGCCATTTGGGGATGGACTCCATAACTGATTTCTTGATT 5 0.125 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.05 0.0 0.0 0.0 0.0 52-53 0.1 0.0 0.0 0.0 0.0 54-55 0.1 0.0 0.0 0.0 0.0 56-57 0.1 0.0 0.0 0.0 0.0 58-59 0.1 0.0 0.0 0.0 0.0 60-61 0.1 0.0 0.0 0.0 0.0 62-63 0.1125 0.0 0.0 0.0 0.0 64-65 0.125 0.0 0.0 0.0 0.0 66-67 0.125 0.0 0.0 0.0 0.0 68-69 0.125 0.0 0.0 0.0 0.0 70-71 0.125 0.0 0.0 0.0 0.0 72-73 0.125 0.0 0.0 0.0 0.0 74-75 0.1375 0.0 0.0 0.0 0.0 76-77 0.2 0.0 0.0 0.0 0.0 78-79 0.275 0.0 0.0 0.0 0.0 80-81 0.325 0.0 0.0 0.0 0.0 82-83 0.3375 0.0 0.0 0.0 0.0 84-85 0.35 0.0 0.0 0.0 0.0 86-87 0.3875 0.0 0.0 0.0 0.0 88-89 0.42500000000000004 0.0 0.0 0.0 0.0 90-91 0.475 0.0 0.0 0.0 0.0 92-93 0.525 0.0 0.0 0.0 0.0 94-95 0.6125 0.0 0.0 0.0 0.0 96-97 0.625 0.0 0.0 0.0 0.0 98-99 0.75 0.0 0.0 0.0 0.0 100-101 0.7875000000000001 0.0 0.0 0.0 0.0 102-103 0.875 0.0 0.0 0.0 0.0 104-105 1.1 0.0 0.0 0.0 0.0 106-107 1.4125 0.0 0.0 0.0 0.0 108-109 1.925 0.0 0.0 0.0 0.0 110-111 2.35 0.0 0.0 0.0 0.0 112-113 2.9125 0.0 0.0 0.0 0.0 114-115 3.125 0.0 0.0 0.0 0.0 116-117 3.425 0.0 0.0 0.0 0.0 118-119 3.675 0.0 0.0 0.0 0.0 120-121 4.0 0.0 0.0 0.0 0.0 122-123 4.475 0.0 0.0 0.0 0.0 124-125 5.0625 0.0 0.0 0.0 0.0 126-127 5.7875 0.0 0.0 0.0 0.0 128-129 6.35 0.0 0.0 0.0 0.0 130-131 6.7125 0.0 0.0 0.0 0.0 132-133 7.0875 0.0 0.0 0.0 0.0 134-135 7.5875 0.0 0.0 0.0 0.0 136-137 8.2875 0.0 0.0 0.0 0.0 138-139 8.925 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ACCATAA 10 0.006830828 145.0 9 GCTACGA 10 0.006830828 145.0 1 AAAAGAT 10 0.006830828 145.0 145 ACGAGAC 10 0.006830828 145.0 4 TACGAGA 10 0.006830828 145.0 3 CTACGAG 10 0.006830828 145.0 2 CGAGACC 10 0.006830828 145.0 5 GAGACCA 10 0.006830828 145.0 6 >>END_MODULE SRR26075381 read2 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR26075381_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.1395 37.0 37.0 37.0 37.0 37.0 2 36.42 37.0 37.0 37.0 37.0 37.0 3 36.3375 37.0 37.0 37.0 37.0 37.0 4 36.431 37.0 37.0 37.0 37.0 37.0 5 36.392 37.0 37.0 37.0 37.0 37.0 6 36.3115 37.0 37.0 37.0 37.0 37.0 7 36.1965 37.0 37.0 37.0 37.0 37.0 8 36.287 37.0 37.0 37.0 37.0 37.0 9 36.271 37.0 37.0 37.0 37.0 37.0 10-14 36.2449 37.0 37.0 37.0 37.0 37.0 15-19 36.251400000000004 37.0 37.0 37.0 37.0 37.0 20-24 36.1243 37.0 37.0 37.0 37.0 37.0 25-29 36.02660000000001 37.0 37.0 37.0 37.0 37.0 30-34 35.9379 37.0 37.0 37.0 37.0 37.0 35-39 35.8896 37.0 37.0 37.0 37.0 37.0 40-44 35.8409 37.0 37.0 37.0 37.0 37.0 45-49 35.8023 37.0 37.0 37.0 37.0 37.0 50-54 35.7217 37.0 37.0 37.0 37.0 37.0 55-59 35.6552 37.0 37.0 37.0 37.0 37.0 60-64 35.736900000000006 37.0 37.0 37.0 37.0 37.0 65-69 35.60680000000001 37.0 37.0 37.0 37.0 37.0 70-74 35.4913 37.0 37.0 37.0 37.0 37.0 75-79 35.462199999999996 37.0 37.0 37.0 37.0 37.0 80-84 35.51 37.0 37.0 37.0 37.0 37.0 85-89 35.4816 37.0 37.0 37.0 37.0 37.0 90-94 35.393 37.0 37.0 37.0 37.0 37.0 95-99 35.423 37.0 37.0 37.0 37.0 37.0 100-104 35.3603 37.0 37.0 37.0 34.6 37.0 105-109 35.3153 37.0 37.0 37.0 34.6 37.0 110-114 35.290800000000004 37.0 37.0 37.0 34.6 37.0 115-119 35.335 37.0 37.0 37.0 37.0 37.0 120-124 35.168699999999994 37.0 37.0 37.0 27.4 37.0 125-129 35.1846 37.0 37.0 37.0 29.8 37.0 130-134 35.1294 37.0 37.0 37.0 27.4 37.0 135-139 34.9878 37.0 37.0 37.0 27.4 37.0 140-144 34.98355 37.0 37.0 37.0 25.0 37.0 145-149 35.00605 37.0 37.0 37.0 25.0 37.0 150-151 34.587625 37.0 37.0 37.0 25.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.0 1101 2 0.0 1101 3 0.0 1101 4 0.0 1101 5 0.0 1101 6 0.0 1101 7 0.0 1101 8 0.0 1101 9 0.0 1101 10-14 0.0 1101 15-19 0.0 1101 20-24 0.0 1101 25-29 0.0 1101 30-34 0.0 1101 35-39 0.0 1101 40-44 0.0 1101 45-49 0.0 1101 50-54 0.0 1101 55-59 0.0 1101 60-64 0.0 1101 65-69 0.0 1101 70-74 0.0 1101 75-79 0.0 1101 80-84 0.0 1101 85-89 0.0 1101 90-94 0.0 1101 95-99 0.0 1101 100-104 0.0 1101 105-109 0.0 1101 110-114 0.0 1101 115-119 0.0 1101 120-124 0.0 1101 125-129 0.0 1101 130-134 0.0 1101 135-139 0.0 1101 140-144 0.0 1101 145-149 0.0 1101 150-151 0.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 12 1.0 13 3.0 14 4.0 15 3.0 16 4.0 17 9.0 18 6.0 19 6.0 20 4.0 21 8.0 22 9.0 23 11.0 24 11.0 25 23.0 26 19.0 27 17.0 28 15.0 29 22.0 30 22.0 31 32.0 32 50.0 33 93.0 34 219.0 35 643.0 36 2563.0 37 203.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.574999999999996 22.075 10.274999999999999 23.075000000000003 2 29.099999999999998 27.825 26.400000000000002 16.675 3 24.625 30.275000000000002 27.250000000000004 17.849999999999998 4 26.625 34.2 21.349999999999998 17.825 5 26.75 37.0 20.175 16.075 6 22.45 37.75 21.349999999999998 18.45 7 24.325 20.575 35.55 19.55 8 24.4 26.35 25.374999999999996 23.875 9 23.625 23.425 31.95 21.0 10-14 24.595 29.294999999999998 25.64 20.47 15-19 25.324999999999996 28.57 25.155 20.95 20-24 25.15 29.005 25.595000000000002 20.25 25-29 24.915000000000003 27.83 26.740000000000002 20.515 30-34 24.779999999999998 27.985 26.155 21.08 35-39 24.64 27.715 27.01 20.635 40-44 25.295 27.265 26.845000000000002 20.595 45-49 25.0 28.435 26.640000000000004 19.925 50-54 24.985 27.93 26.484999999999996 20.599999999999998 55-59 25.39 28.560000000000002 26.540000000000003 19.509999999999998 60-64 24.64 27.644999999999996 27.205000000000002 20.51 65-69 24.335 28.715000000000003 26.515 20.435 70-74 24.995 27.875 26.875 20.255000000000003 75-79 23.52 28.83 26.68 20.97 80-84 24.9 28.485 26.169999999999998 20.445 85-89 23.549999999999997 28.185 28.09 20.175 90-94 24.615000000000002 28.215 27.105 20.064999999999998 95-99 25.119999999999997 27.405 27.11 20.365 100-104 25.36 27.58 26.474999999999998 20.585 105-109 25.419999999999998 28.060000000000002 27.27 19.25 110-114 25.330000000000002 28.03 26.595000000000002 20.044999999999998 115-119 25.41 28.599999999999998 25.729999999999997 20.26 120-124 24.79 28.939999999999998 27.339999999999996 18.93 125-129 25.895000000000003 27.54 27.224999999999998 19.34 130-134 26.465 29.225 25.580000000000002 18.73 135-139 26.61 28.044999999999998 26.215 19.13 140-144 25.946297314865742 27.931396569828493 26.856342817140856 19.26596329816491 145-149 26.173926088913333 28.564284642696403 25.893884082612388 19.367905185777868 150-151 25.240655081885237 28.066008251031377 28.141017627203404 18.552319039879986 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.5 2 0.5 3 0.0 4 0.0 5 0.5 6 1.5 7 1.5 8 0.5 9 0.0 10 1.5 11 1.5 12 0.5 13 1.0 14 0.5 15 1.0 16 1.5 17 1.0 18 1.0 19 1.0 20 0.5 21 0.0 22 0.5 23 0.5 24 1.0 25 2.0 26 1.5 27 2.5 28 3.5 29 6.0 30 13.0 31 16.5 32 18.0 33 24.0 34 32.0 35 50.0 36 61.0 37 80.0 38 108.0 39 147.0 40 210.0 41 240.0 42 242.5 43 239.5 44 241.0 45 248.5 46 271.5 47 263.0 48 235.0 49 224.0 50 194.0 51 177.5 52 155.0 53 109.0 54 79.0 55 64.5 56 51.0 57 32.0 58 14.5 59 12.0 60 8.0 61 11.0 62 14.5 63 10.0 64 6.0 65 2.0 66 1.5 67 4.0 68 3.5 69 1.5 70 2.0 71 1.0 72 1.0 73 1.0 74 1.0 75 1.0 76 0.5 77 0.0 78 0.5 79 2.5 80 2.5 81 1.5 82 2.5 83 2.5 84 3.0 85 5.0 86 4.0 87 2.0 88 2.5 89 1.5 90 0.0 91 0.5 92 2.0 93 2.5 94 1.0 95 0.0 96 0.0 97 0.5 98 0.5 99 0.0 100 3.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.005 145-149 0.015 150-151 0.0125 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 58.8 #Duplication Level Percentage of deduplicated Percentage of total 1 61.39455782312925 36.1 2 22.27891156462585 26.200000000000003 3 8.58843537414966 15.15 4 4.209183673469387 9.9 5 1.6581632653061225 4.875 6 0.935374149659864 3.3000000000000003 7 0.4251700680272109 1.7500000000000002 8 0.2976190476190476 1.4000000000000001 9 0.04251700680272109 0.22499999999999998 >10 0.17006802721088435 1.0999999999999999 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG 12 0.3 No Hit GACACCGATGGCGAGTATGAAAAACTACTTCTGGAATTAGTTGGACATGA 11 0.27499999999999997 No Hit CAAGAGTGGTTCACGCAAAAGGACAGCGAGAGAAAAGTAAAGGCAAGAGT 11 0.27499999999999997 No Hit GGTGAGGATGATGCCAAGGAAGCCAAACATCTGCCACTTCCTTCAGCATG 10 0.25 No Hit GAAAAAGATTGAGGATGCAATCGATCAGGCCATTCAATGGCTGGACAGCA 9 0.22499999999999998 No Hit GAGGATTTTAGCAGAAGAATATGGGATCGAAGAACATGGATTTGAAGAGG 8 0.2 No Hit GGGAAAGCTAATTTCGTCGCTGCTAATGATCAAGCAGCTTTTAACTTCTT 8 0.2 No Hit GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGT 8 0.2 No Hit GGTGGCCATTAAGATTGTGGGAACCAATGCGGAGGAGCAGCAAAAAATGC 8 0.2 No Hit GCTCTCGCAATCGCTGCTTCTTTGTCTGTCTTTGGGTCGATCCGAAAGAG 8 0.2 No Hit CAGATCTCTCTCTCTGTCTGTCTTTTTTTCTCTATCTAGAAAGGAAAACA 8 0.2 No Hit CTGGATAGCATGGGCAGAACCGCAGTATAACTTCAGCCCTATGATCGATG 8 0.2 No Hit GTGTCTTCCTCAAAGTAAACAAGGCAACCGTGAATATGCTTCACAGGGTT 7 0.17500000000000002 No Hit CATCATTATGATGGCCCCAAGTTTCGCCTTGAGCTCTCAGTTCCTGAGAC 7 0.17500000000000002 No Hit GTGTATCCCTACAAGTCAAGTGATTCACCTATGTCAGTTGGAACCTGACA 7 0.17500000000000002 No Hit TCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAATGGACGGGAGGTAT 7 0.17500000000000002 No Hit CAACTTGAAGGCAAGGCTTTGTCAAGTGGAAACCAGTGGTTCTACTTTAG 7 0.17500000000000002 No Hit GGGAATGTTTCAGAACGAAACAAGAAGTCACCTCCCATGTCAATTAAGGA 7 0.17500000000000002 No Hit CATATCGGCTGCTTCAGTCTCTTGCCTCCAAGCCCCCAACTCGGGAAAAT 7 0.17500000000000002 No Hit GCTGGATTGGAGTCGAGTGGTGGTTTGCCAGCGGAGATTTCTGTTGATGG 7 0.17500000000000002 No Hit GAAATCCAAAGCCAAAAATATGCTTCTTAGGAGCTCATCAACGCCAATTC 7 0.17500000000000002 No Hit CTCTAACTCTGACTTTTTAGGGTGGCATATCTGTATTATAAATCCTGGTC 7 0.17500000000000002 No Hit GAAAATTATCTGCAATCCTCTCTTGATTCTCGCTGCTGTTTCCTAGCTAA 6 0.15 No Hit ATCTGATGGGAGGATAAGCATGGCAGGTCTTAGTTCCAGGACAGTCCCTC 6 0.15 No Hit ACTGAGCCTGAGGAACCTGTTGAAGCAGTTGCAGAGGCAGAACCTGAAAT 6 0.15 No Hit GCTCGTTCTCCTGCTTTCTATCGCGACCAACAAAAAAAAAAAAAAAAAAG 6 0.15 No Hit CTAAGCTTCTCAACATCAATCTTATTCTACCCATCGCTCTTGAGCTCCTT 6 0.15 No Hit AAAGTCTCCGAGAACTGGATCAGCGCCACCCTCCTCCTCGCTCCTCTCGT 6 0.15 No Hit GGGCTTTCCTCCAGCCTGGGGGCAGAGGGAGCCACTAGCAAAAGCCAAAC 6 0.15 No Hit CAATAAATTCCAAACTCCAAAAAGGAAAATAAAATTAAAAGAACGCGAGA 6 0.15 No Hit GTTTTCCTACAACATTACAGCCAGGAAGCTACTTCAACTGGTGGGAGAGG 6 0.15 No Hit GATGGAAGGATCAATATCAGTGGTCTTAGGTCTCGTTACTTTATTGTATA 6 0.15 No Hit GTATCAGAAAGCTACTTACCAGAATCCTCAAGGAAATCAGGGAGAGAATG 6 0.15 No Hit GTGAAAGTGACCAGAAGAATTACCTGTTGCAGCTCTTAGAGACTATGGAT 6 0.15 No Hit GTGCAAAGCACTACAAGAAGTTGCTGCCAGCACTTGGGTCTGATAAGATG 6 0.15 No Hit GGAAACAGGAACGATGGAGTTATGGTTGCTGGGGTGAGTGATGCTCTGTG 6 0.15 No Hit GCAAGGAGGAGAAAGATTTGCTTTTCACAGCCAAGAAATCAAAGCTGTAC 6 0.15 No Hit TGATACCAGCATATAAGAAAACTGAAGATTCCCCTCCCTCTCCAGCTGAA 6 0.15 No Hit GTTTACAATGGCAAGACCTTTAATCAAGTTGAAATCAAGCCTGAAATGAT 6 0.15 No Hit ATAAAATATAAACTCCTTCCTAATTCTCATTTCGTTCAAGATGCGTGAGA 6 0.15 No Hit CGGGAATTCTGAATTAGATTCCCGATTTCAAAGTGGAAATGTCAGCAAAG 6 0.15 No Hit AGCAAAAGCAAGGTTTGGGCACATTGATGGGGATCACCTCACGCTGTTGA 6 0.15 No Hit AGCCTTTGAATTTTTTGATCAAAACCAAAGTGGGCACATAGAAATCGATG 6 0.15 No Hit GGAATAGTATTTTCTCGCATCCTAGAAGCTGTATCTTTTCAGGGTAGTGG 6 0.15 No Hit AGATCAAAATCTCTCCCTCTGCCTACACAAGTACGAGTAAAAATGTACGG 5 0.125 No Hit GTTCTCCACCATTATGATGGCCCTAAGTTCCGCCTCGAGCTCAACATACC 5 0.125 No Hit AACAAACCAGTCACCAACAGCAGAGACACTCAAGAGGTCAAGTTAGAGCA 5 0.125 No Hit CTACTGACCAATCCCTTCAAGAAGCGTTTAGCCAGTACGGTGAAATCATC 5 0.125 No Hit GAACTACTACAGGTGCACCCACAAGTTCGACCAACATTGCCAAGCAACCA 5 0.125 No Hit GAGAAAGGGAGCACAGGGAGAAGCTGGCTTCAACTATTGTTCTTGTCAAT 5 0.125 No Hit CAAAGCTCTCTTCTTCTTTGCCTTGTTGTCCTTCTCAGCTGTGTCGGTCA 5 0.125 No Hit CACTCTCTAATGGCTCTACGCTCTCTCGTTTCTCGAAAAACCCTAGGCCT 5 0.125 No Hit GAGAAATTGTTCTTGCTTCCTTGGATGGATTTAATAAGGTGTGTGATGCT 5 0.125 No Hit TGAAGAATTCATCAGAAGGTTTTATGAGCAGCTAAGGGTACAAAGTCGCA 5 0.125 No Hit GGAGAAAACTTAGATGCTGGGACAGAGACTAATTCTAATCTTTATAATGA 5 0.125 No Hit AATGAAGGTTGTCGCCGCTTACTTGCTCGCCGTTCTCGGTGGCAACACCT 5 0.125 No Hit AACATCAGCAAAACCCAGGGTCTCTAGGAAGAACAATGATCAACAATCCA 5 0.125 No Hit GCAACACAGAGAGTAAAGGAGAGAGGGAGAGAAAACCCTAAGCCGCCGCT 5 0.125 No Hit AGTAAGCTTGCTAATACTGATGCCTACCCACCCAAGTGTGATGACAGTCT 5 0.125 No Hit CTCTCATCTCTCGCCACCAAGGGCTTCGATGCCACCGACGTGGTAGCCCT 5 0.125 No Hit ATTGGAGAAATTTTGAAAGGCATACGGTGTTATTTTGACAAAGCATTGCC 5 0.125 No Hit GCGGGGCAGATGAGAATGGAGAAGAGGAACAAGAAGATGATGAAGATGAT 5 0.125 No Hit GAATAAATTTGTTCGTGAGAGGGATCTTCGAGTGGATGATAAGGTCACAA 5 0.125 No Hit ACAAGCTGCGTAGAGAGAGAAACAAGAACAAAACAATTTCTTTTGGAGAG 5 0.125 No Hit ATGTATTTGGCCACATGGGTCTCAGTGACACAGATATTGTTGCCCTATCC 5 0.125 No Hit GGGACGGCGAGCTCCGCCTCTGGGACTTGGCTACTGGTGTCTCTGCTCGC 5 0.125 No Hit CAAGAAGTTCAGTTTTAGAGGAGTTGATTTGGATGCTCTCTTGGACATGT 5 0.125 No Hit AGATCCCTAAGTTTGAAGACAAAACGACTCCAGAATACAAGCCTAAATTT 5 0.125 No Hit AAAAAATATATCTTGATCCATCTCTAATCGGAACAGGAATCCATTGAGAA 5 0.125 No Hit GGGAGTTTCATTAAATGCTAGCGCACAATCTCAGTTACAGTCTCCTAATG 5 0.125 No Hit TGTGAACCTTCTCATTTATGGAATGTTAGGGATATTTAAAGGAACTTGGA 5 0.125 No Hit ATTGAGTTCAGATCACCGAGCTCCCACAATTCGATCAGACACTAGACATT 5 0.125 No Hit GCCAATTTTCAATCTCCCTCGCTCCCTCAACACCTTCTCTTGTCCACAGA 5 0.125 No Hit CAATGCGGATCGCAGTGGCGAAGATTAAATACAACTCTTGGAGTGGTTCT 5 0.125 No Hit GGAAGAAGTCAAAGCACTATGGTGTCAAGCCGTACAGAATTGATTCTGAT 5 0.125 No Hit GAGAACAAACCTGTAACCAACAGCAGGGACACTCAAGAGGTCAAGTTAGA 5 0.125 No Hit GCTTGTTGTTCATTACCTGAAGCGAAAGGTCCTTGGTCTTCCATTGCCAG 5 0.125 No Hit TGATCTTAGCCCTGAAGTTACAGATGCTACATTGTATGCATGTTTCTCTG 5 0.125 No Hit GGAGAATCCTCCCGAAACCGCCCCCTTGACTATTTTCTACAACGGAACCG 5 0.125 No Hit GCAGATCTTTGTTAAGACCCTTACTGGCAAGACAATCACCTTGGAGGTGG 5 0.125 No Hit GTTAAGAGTAAAAAGAAGAAACAAGACACACCATTCCCTCCTCCTCAACA 5 0.125 No Hit ACCTCGTCGATAACGGCTTCACTTACTGTGTAGTTGCAGTGGAGTCTGTG 5 0.125 No Hit GCAAAACCTAGCCTTATTTGGCTCCTCATCTCACTATTGTACATTGCAAG 5 0.125 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.05 0.0 0.0 0.0 0.0 52-53 0.1 0.0 0.0 0.0 0.0 54-55 0.1 0.0 0.0 0.0 0.0 56-57 0.1 0.0 0.0 0.0 0.0 58-59 0.1 0.0 0.0 0.0 0.0 60-61 0.1 0.0 0.0 0.0 0.0 62-63 0.1125 0.0 0.0 0.0 0.0 64-65 0.125 0.0 0.0 0.0 0.0 66-67 0.125 0.0 0.0 0.0 0.0 68-69 0.125 0.0 0.0 0.0 0.0 70-71 0.125 0.0 0.0 0.0 0.0 72-73 0.125 0.0 0.0 0.0 0.0 74-75 0.1375 0.0 0.0 0.0 0.0 76-77 0.2 0.0 0.0 0.0 0.0 78-79 0.275 0.0 0.0 0.0 0.0 80-81 0.325 0.0 0.0 0.0 0.0 82-83 0.3375 0.0 0.0 0.0 0.0 84-85 0.35 0.0 0.0 0.0 0.0 86-87 0.3875 0.0 0.0 0.0 0.0 88-89 0.42500000000000004 0.0 0.0 0.0 0.0 90-91 0.475 0.0 0.0 0.0 0.0 92-93 0.5125 0.0 0.0 0.0 0.0 94-95 0.5874999999999999 0.0 0.0 0.0 0.0 96-97 0.6 0.0 0.0 0.0 0.0 98-99 0.725 0.0 0.0 0.0 0.0 100-101 0.7625 0.0 0.0 0.0 0.0 102-103 0.825 0.0 0.0 0.0 0.0 104-105 1.025 0.0 0.0 0.0 0.0 106-107 1.3375 0.0 0.0 0.0 0.0 108-109 1.85 0.0 0.0 0.0 0.0 110-111 2.275 0.0 0.0 0.0 0.0 112-113 2.8375 0.0 0.0 0.0 0.0 114-115 3.05 0.0 0.0 0.0 0.0 116-117 3.375 0.0 0.0 0.0 0.0 118-119 3.625 0.0 0.0 0.0 0.0 120-121 3.925 0.0 0.0 0.0 0.0 122-123 4.4375 0.0 0.0 0.0 0.0 124-125 5.075 0.0 0.0 0.0 0.0 126-127 5.8875 0.0 0.0 0.0 0.0 128-129 6.4375 0.0 0.0 0.0 0.0 130-131 6.7875 0.0 0.0 0.0 0.0 132-133 7.1625 0.0 0.0 0.0 0.0 134-135 7.65 0.0 0.0 0.0 0.0 136-137 8.3375 0.0 0.0 0.0 0.0 138-139 8.9625 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATGCTTG 10 0.006830828 145.0 2 CTTGCAA 10 0.006830828 145.0 5 CATGCTT 10 0.006830828 145.0 1 >>END_MODULE Read 667663 spots for SRR26075381.sra Written 667663 spots for SRR26075381.sra Read 667663 spots for SRR26075381.sra Written 667663 spots for SRR26075381.sra Read 667663 spots for SRR26075381.sra Written 667663 spots for SRR26075381.sra Read 667663 spots for SRR26075381.sra Written 667663 spots for SRR26075381.sra Read 667663 spots for SRR26075381.sra Written 667663 spots for SRR26075381.sra Read 667663 spots for SRR26075381.sra Written 667663 spots for SRR26075381.sra Read 667663 spots for SRR26075381.sra Written 667663 spots for SRR26075381.sra Read 667663 spots for SRR26075381.sra Written 667663 spots for SRR26075381.sra Read 667663 spots for SRR26075381.sra Written 667663 spots for SRR26075381.sra Read 667664 spots for SRR26075381.sra Written 667664 spots for SRR26075381.sra Read 667663 spots for SRR26075381.sra Written 667663 spots for SRR26075381.sra Read 667663 spots for SRR26075381.sra Written 667663 spots for SRR26075381.sra Read 667663 spots for SRR26075381.sra Written 667663 spots for SRR26075381.sra Read 667663 spots for SRR26075381.sra Written 667663 spots for SRR26075381.sra Read 667663 spots for SRR26075381.sra Written 667663 spots for SRR26075381.sra Read 667663 spots for SRR26075381.sra Written 667663 spots for SRR26075381.sra Read 667663 spots for SRR26075381.sra Written 667663 spots for SRR26075381.sra Read 667663 spots for SRR26075381.sra Written 667663 spots for SRR26075381.sra Read 667663 spots for SRR26075381.sra Written 667663 spots for SRR26075381.sra Read 667663 spots for SRR26075381.sra Written 667663 spots for SRR26075381.sra SRR ids: ['SRR26075381.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_ksej2yyy SRR26075381.sra spots: 13353261 blocks: [[1, 667663], [667664, 1335326], [1335327, 2002989], [2002990, 2670652], [2670653, 3338315], [3338316, 4005978], [4005979, 4673641], [4673642, 5341304], [5341305, 6008967], [6008968, 6676630], [6676631, 7344293], [7344294, 8011956], [8011957, 8679619], [8679620, 9347282], [9347283, 10014945], [10014946, 10682608], [10682609, 11350271], [11350272, 12017934], [12017935, 12685597], [12685598, 13353261]] SRR26075381 file size 4924449 SRR26075381 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o SRR26075381 SRR26075381_1.fastq SRR26075381_2.fastq Input file: SRR26075381_1.fastq Paired file: SRR26075381_2.fastq trimmed: SRR26075381-trimmed-pair1.fastq, SRR26075381-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Tue Feb 11 23:37:57 2025 >> started Tue Feb 11 23:38:13 2025 >> done (15.788s) 13353261 read pairs processed; of these: 50 ( 0.00%) short read pairs filtered out after trimming by size control 72079 ( 0.54%) empty read pairs filtered out after trimming by size control 13281132 (99.46%) read pairs available; of these: 1637193 (12.33%) trimmed read pairs available after processing 11643939 (87.67%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 18 6 0.00% 19 3 0.00% 20 2 0.00% 21 11 0.00% 22 6 0.00% 23 9 0.00% 24 7 0.00% 25 4 0.00% 26 12 0.00% 27 21 0.00% 28 11 0.00% 29 14 0.00% 30 16 0.00% 31 14 0.00% 32 14 0.00% 33 15 0.00% 34 18 0.00% 35 31 0.00% 36 13 0.00% 37 17 0.00% 38 13 0.00% 39 17 0.00% 40 24 0.00% 41 11 0.00% 42 25 0.00% 43 17 0.00% 44 27 0.00% 45 27 0.00% 46 28 0.00% 47 23 0.00% 48 34 0.00% 49 28 0.00% 50 65 0.00% 51 42 0.00% 52 49 0.00% 53 49 0.00% 54 82 0.00% 55 56 0.00% 56 59 0.00% 57 79 0.00% 58 100 0.00% 59 89 0.00% 60 106 0.00% 61 151 0.00% 62 202 0.00% 63 139 0.00% 64 210 0.00% 65 217 0.00% 66 248 0.00% 67 263 0.00% 68 336 0.00% 69 401 0.00% 70 421 0.00% 71 508 0.00% 72 558 0.00% 73 758 0.01% 74 734 0.01% 75 807 0.01% 76 984 0.01% 77 1034 0.01% 78 1212 0.01% 79 1443 0.01% 80 1626 0.01% 81 1827 0.01% 82 2064 0.02% 83 2358 0.02% 84 2652 0.02% 85 2812 0.02% 86 3167 0.02% 87 3460 0.03% 88 3947 0.03% 89 4027 0.03% 90 4570 0.03% 91 5176 0.04% 92 5591 0.04% 93 6146 0.05% 94 6840 0.05% 95 7341 0.06% 96 7977 0.06% 97 8472 0.06% 98 8492 0.06% 99 9327 0.07% 100 9787 0.07% 101 10528 0.08% 102 11246 0.08% 103 12310 0.09% 104 12667 0.10% 105 13731 0.10% 106 14672 0.11% 107 15268 0.11% 108 15829 0.12% 109 16608 0.13% 110 16541 0.12% 111 17933 0.14% 112 18469 0.14% 113 19159 0.14% 114 20043 0.15% 115 21751 0.16% 116 22118 0.17% 117 22947 0.17% 118 24571 0.19% 119 24667 0.19% 120 25093 0.19% 121 25923 0.20% 122 26902 0.20% 123 27203 0.20% 124 28796 0.22% 125 29199 0.22% 126 30827 0.23% 127 31918 0.24% 128 32482 0.24% 129 33654 0.25% 130 34274 0.26% 131 34686 0.26% 132 35630 0.27% 133 36612 0.28% 134 37585 0.28% 135 38929 0.29% 136 39226 0.30% 137 40106 0.30% 138 40860 0.31% 139 42139 0.32% 140 41779 0.31% 141 43220 0.33% 142 43723 0.33% 143 45336 0.34% 144 46418 0.35% 145 46465 0.35% 146 47292 0.36% 147 48841 0.37% 148 50064 0.38% 149 50168 0.38% 150 51206 0.39% 151 11643939 87.67% 13281132 reads passed initial QC criterion=sequence-density sequence-density=0.28 sequence-density-rank=1 fanout-score=2.80 fanout-score-rank=36 prefix-density=0.29 prefix-fanout=2.7 sequence=GTGGACTCCTTCTGGAT criterion=fanout-score sequence-density=0.05 sequence-density-rank=37 fanout-score=187.57 fanout-score-rank=1 prefix-density=0.41 prefix-fanout=21.7 sequence=AAAACAAAACACAGTACGTATTTACATGGGCAACCTTGGTTGAAGGCAACCTCATCAACGATGCTCGCTCTTCGTCTCTCCACTGTACATCCAGTCATAGACAGTGGGTGTGTTAGGCTGTGGCTTGTCAAAAACATGAGCACCAATATTCTTAGTAGCAAGGTTGCTACCAGGGTGGAACACGCTCCTCCAAACATTGCTACGCGCCGACACTGGGGTTGTAGGGGTCACTGGTGTCGTCGGTGTCCCTGGAGTTCCTGGCATAGTCATGGACCTCTGAAACTTATTAACAGGACTGCTCCCCTCTCCGACGTCAATATCTTTGATGTTAAGTGGTCTGGTGCTGATCTTTCTAAGCTTGCCAAGGCCACGTTCTGGCTGAGGTCCAGCAACAACATCATCCCACATCTTGTCTAGTAAAACC criterion=sequence-density sequence-density=0.21 sequence-density-rank=1 fanout-score=4.87 fanout-score-rank=26 prefix-density=0.26 prefix-fanout=3.9 sequence=TGCAAGTGCGGCAGTGGCTGCAA criterion=fanout-score sequence-density=0.01 sequence-density-rank=42 fanout-score=174.63 fanout-score-rank=1 prefix-density=0.15 prefix-fanout=6.0 sequence=TCCTGCTCTCGCAATCGCTGCTTCTTTGTCTGTCTTTGGGTCGATCCGAAAGAGAGGAGCTCTTCTGCGCAATCATGTTGGTCTATCAAGATCTTCTCTCTGGTGATGAGCTTCTCTCGGATTCGTTCCCATACAAGGAGATTGAGAATGG SRR26075381 testing PE reads STAR mapping to Ensembl genome Started job on | Feb 11 23:39:05 Started mapping on | Feb 11 23:39:06 Finished on | Feb 11 23:41:26 Mapping speed, Million of reads per hour | 341.51 Number of input reads | 13281132 Average input read length | 296 UNIQUE READS: Uniquely mapped reads number | 11851701 Uniquely mapped reads % | 89.24% Average mapped length | 294.73 Number of splices: Total | 9755891 Number of splices: Annotated (sjdb) | 9506486 Number of splices: GT/AG | 9584243 Number of splices: GC/AG | 127174 Number of splices: AT/AC | 10799 Number of splices: Non-canonical | 33675 Mismatch rate per base, % | 0.41% Deletion rate per base | 0.03% Deletion average length | 3.07 Insertion rate per base | 0.02% Insertion average length | 2.54 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 330510 % of reads mapped to multiple loci | 2.49% Number of reads mapped to too many loci | 37913 % of reads mapped to too many loci | 0.29% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 7.67% % of reads unmapped: other | 0.32% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 1098921 1098921 1098921 N_multimapping 330510 330510 330510 N_noFeature 309279 11715487 378645 N_ambiguous 133908 775 66730 UnstrandedReadsAssigned:11408514 PositiveStrandReadsAssigned:135439 NegativeStrandReadsAssigned:11406326 Dataset is classified negative stranded MeadianReadLen=151 20thPercentileLength=151 echo kmer=147 SRR26075381 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,400 [index] number of k-mers: 62,057,036 [index] number of equivalence classes: 130,681 [quant] running in paired-end mode [quant] will process pair 1: SRR26075381-trimmed-pair1.fastq SRR26075381-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 13,281,132 reads, 11,595,749 reads pseudoaligned [quant] estimated average fragment length: 222.803 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,157 rounds 52401 SRR26075381.ke.tsv 34699 SRR26075381.se.tsv 87100 total ==> SRR26075381.ke.tsv <== target_id length eff_length est_counts tpm Potri.005G200100.1.v4.1 2018 1796.2 1987 69.9405 Potri.005G024800.1.v4.1 1035 813.197 4884 379.721 Potri.004G059700.1.v4.1 961 739.225 7 0.598696 Potri.007G009000.2.v4.1 1416 1194.2 0 0 Potri.003G141000.2.v4.1 2943 2721.2 452 10.5018 Potri.016G087400.1.v4.1 270 84.6501 1780.02 1329.48 Potri.015G069301.1.v4.1 564 344.253 0 0 Potri.010G195200.1.v4.1 1773 1551.2 113 4.60571 Potri.012G127500.1.v4.1 977 755.225 11788 986.845 ==> SRR26075381.se.tsv <== Potri.001G166300.v4.1 0 Potri.001G448400.v4.1 124 Potri.001G233950.v4.1 0 Potri.001G122700.v4.1 204 Potri.001G212900.v4.1 0 Potri.001G182400.v4.1 0 Potri.001G256600.v4.1 0 Potri.001G040500.v4.1 17 Potri.001G416900.v4.1 0 Potri.001G452600.v4.1 355 SRR26075381 completed mapping pipeline successfully