Starting /dee2/code/volunteer_pipeline.sh SRR26075382 current disk space = 3051992055808 free memory = 1432022688 SRR26075382 SRAfilesize 1eee336d436e29fe3d3642bc5ec67b5a SRR26075382.sra SRR26075382.sra file validated SRR26075382 is paired end SRR26075382 is conventional basespace SRR26075382 read1 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR26075382_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.70725 37.0 37.0 37.0 37.0 37.0 2 36.662 37.0 37.0 37.0 37.0 37.0 3 36.6225 37.0 37.0 37.0 37.0 37.0 4 36.7315 37.0 37.0 37.0 37.0 37.0 5 36.729 37.0 37.0 37.0 37.0 37.0 6 36.6445 37.0 37.0 37.0 37.0 37.0 7 36.578 37.0 37.0 37.0 37.0 37.0 8 36.6585 37.0 37.0 37.0 37.0 37.0 9 36.6955 37.0 37.0 37.0 37.0 37.0 10-14 36.655150000000006 37.0 37.0 37.0 37.0 37.0 15-19 36.587399999999995 37.0 37.0 37.0 37.0 37.0 20-24 36.494 37.0 37.0 37.0 37.0 37.0 25-29 36.446799999999996 37.0 37.0 37.0 37.0 37.0 30-34 36.42439999999999 37.0 37.0 37.0 37.0 37.0 35-39 36.33819999999999 37.0 37.0 37.0 37.0 37.0 40-44 36.298899999999996 37.0 37.0 37.0 37.0 37.0 45-49 36.27419999999999 37.0 37.0 37.0 37.0 37.0 50-54 36.182100000000005 37.0 37.0 37.0 37.0 37.0 55-59 36.1522 37.0 37.0 37.0 37.0 37.0 60-64 36.1237 37.0 37.0 37.0 37.0 37.0 65-69 36.061099999999996 37.0 37.0 37.0 37.0 37.0 70-74 36.0179 37.0 37.0 37.0 37.0 37.0 75-79 35.9985 37.0 37.0 37.0 37.0 37.0 80-84 36.0064 37.0 37.0 37.0 37.0 37.0 85-89 35.911699999999996 37.0 37.0 37.0 37.0 37.0 90-94 35.889599999999994 37.0 37.0 37.0 37.0 37.0 95-99 35.8526 37.0 37.0 37.0 37.0 37.0 100-104 35.857600000000005 37.0 37.0 37.0 37.0 37.0 105-109 35.714600000000004 37.0 37.0 37.0 37.0 37.0 110-114 35.633300000000006 37.0 37.0 37.0 37.0 37.0 115-119 35.5663 37.0 37.0 37.0 37.0 37.0 120-124 35.5748 37.0 37.0 37.0 37.0 37.0 125-129 35.3448 37.0 37.0 37.0 34.6 37.0 130-134 35.2856 37.0 37.0 37.0 34.6 37.0 135-139 35.1037 37.0 37.0 37.0 29.8 37.0 140-144 34.973400000000005 37.0 37.0 37.0 25.0 37.0 145-149 34.869099999999996 37.0 37.0 37.0 25.0 37.0 150-151 34.71975 37.0 37.0 37.0 25.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.0 1101 2 0.0 1101 3 0.0 1101 4 0.0 1101 5 0.0 1101 6 0.0 1101 7 0.0 1101 8 0.0 1101 9 0.0 1101 10-14 0.0 1101 15-19 0.0 1101 20-24 0.0 1101 25-29 0.0 1101 30-34 0.0 1101 35-39 0.0 1101 40-44 0.0 1101 45-49 0.0 1101 50-54 0.0 1101 55-59 0.0 1101 60-64 0.0 1101 65-69 0.0 1101 70-74 0.0 1101 75-79 0.0 1101 80-84 0.0 1101 85-89 0.0 1101 90-94 0.0 1101 95-99 0.0 1101 100-104 0.0 1101 105-109 0.0 1101 110-114 0.0 1101 115-119 0.0 1101 120-124 0.0 1101 125-129 0.0 1101 130-134 0.0 1101 135-139 0.0 1101 140-144 0.0 1101 145-149 0.0 1101 150-151 0.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 22 4.0 23 3.0 24 8.0 25 7.0 26 6.0 27 18.0 28 19.0 29 27.0 30 28.0 31 50.0 32 67.0 33 117.0 34 187.0 35 443.0 36 2810.0 37 206.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.605454090567925 16.08706529897423 7.380535401551164 35.92694520890668 2 20.7 12.049999999999999 33.7 33.550000000000004 3 17.549999999999997 18.0 29.425 35.025 4 20.175 24.65 26.1 29.075 5 23.75 30.325000000000003 24.55 21.375 6 24.175 32.775 21.75 21.3 7 17.2 29.775000000000002 37.7 15.325 8 16.525000000000002 28.249999999999996 30.15 25.074999999999996 9 17.65 24.875 34.449999999999996 23.025000000000002 10-14 19.1809590479524 29.381469073453676 28.206410320516024 23.231161558077904 15-19 20.9 28.544999999999998 27.450000000000003 23.105 20-24 20.044999999999998 28.025 28.535 23.395 25-29 20.535 28.175 27.650000000000002 23.64 30-34 20.155 28.275 27.22 24.349999999999998 35-39 20.810000000000002 27.83 27.625 23.735 40-44 20.169999999999998 29.705 26.634999999999998 23.49 45-49 20.875 27.675 27.495000000000005 23.955000000000002 50-54 20.8 27.775 27.994999999999997 23.43 55-59 20.830000000000002 27.935 27.605 23.630000000000003 60-64 20.965 28.615000000000002 27.42 23.0 65-69 20.65 28.355000000000004 26.88 24.115000000000002 70-74 21.19 27.765 27.38 23.665 75-79 20.775 27.839999999999996 26.99 24.395 80-84 20.674999999999997 28.555000000000003 26.915 23.855 85-89 20.794999999999998 27.425 27.565 24.215 90-94 21.415 27.005000000000003 27.045 24.535 95-99 20.974999999999998 26.784999999999997 27.27 24.97 100-104 20.49 28.16 27.615000000000002 23.735 105-109 21.095 27.605 27.279999999999998 24.02 110-114 22.285 26.840000000000003 27.08 23.794999999999998 115-119 21.495 27.685 27.605 23.215 120-124 21.285 27.74 26.700000000000003 24.275 125-129 21.41 27.51 27.125 23.955000000000002 130-134 21.545 27.950000000000003 26.57 23.935000000000002 135-139 21.325 27.275 26.875 24.525 140-144 20.945 27.29 27.26 24.505 145-149 21.665 27.389999999999997 26.845000000000002 24.099999999999998 150-151 22.2625 25.275 28.0625 24.4 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.5 12 1.0 13 0.5 14 0.5 15 1.0 16 0.5 17 0.0 18 0.0 19 0.0 20 0.5 21 0.5 22 3.0 23 3.0 24 0.5 25 5.5 26 8.5 27 12.0 28 17.0 29 16.5 30 24.5 31 31.5 32 33.0 33 33.0 34 40.5 35 60.0 36 81.0 37 93.5 38 113.5 39 133.0 40 165.0 41 193.0 42 206.0 43 241.0 44 277.0 45 276.0 46 254.0 47 254.5 48 234.0 49 194.5 50 174.0 51 151.0 52 119.0 53 104.5 54 86.5 55 66.5 56 62.5 57 55.0 58 35.0 59 20.5 60 19.0 61 19.5 62 16.5 63 9.0 64 10.0 65 11.5 66 7.5 67 6.5 68 3.5 69 0.0 70 1.5 71 4.0 72 3.0 73 1.0 74 1.5 75 1.0 76 0.5 77 0.5 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.075 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.005 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150-151 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 62.875 #Duplication Level Percentage of deduplicated Percentage of total 1 65.20874751491054 41.0 2 21.391650099403577 26.900000000000002 3 7.75347912524851 14.625 4 2.862823061630219 7.199999999999999 5 1.4314115308151094 4.5 6 0.8349900596421471 3.15 7 0.2385685884691849 1.05 8 0.11928429423459246 0.6 9 0.11928429423459246 0.675 >10 0.039761431411530816 0.3 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CCCAGATCAGAATTAGGCTGTGCAGTGAAGATGATAACATCAGAGTCAGC 12 0.3 No Hit ACAGAATCTAAGGTTGGAGAGTCACTGATTTACTTTTGTTCAAAACAAAT 9 0.22499999999999998 No Hit CCGTCAATCATTCAAACAACCATCAGCCTTGAATACCCAAATGAAGGATG 9 0.22499999999999998 No Hit GCACAGGATAGAACTTGTAGAACCTCAGATTTTCTATCGCTCTTCTAGTT 9 0.22499999999999998 No Hit CTGCTAGAGACCTTTATACATGTTGGCTATGATGATGACAAACAAGATCT 8 0.2 No Hit CCACTGTCGGGTCTAATGAAGCTCTTGAGATCGAAAGAGGCAGCACTATG 8 0.2 No Hit TGTTGTCAGTTTGTTTTTTCATGCTTTTCTCAACAATAGAGACCACATCT 8 0.2 No Hit GTCGGATTTTACTTTGAGCCCTCCAAAAACGGACCCCTTTAGGGTTCTTC 7 0.17500000000000002 No Hit GCAGCGGCAACTTGTAGTATATCAGGATGAAAAAGGATACCCAACCATGA 7 0.17500000000000002 No Hit CCTCCAACAACTTGCTCTGCTCGGCATTTGCGCATCTCAGTTGTTCATCC 7 0.17500000000000002 No Hit AGCTGGGAATCTTTCTTTTACTTTCATCGAAATACTACGGATTCATATTA 7 0.17500000000000002 No Hit CCGGAGATTATCTGATATTAGAGATAGCTTATCAGCTACAATATATTTGA 7 0.17500000000000002 No Hit CCCTTGTAAACAACGCAACCCGTCCATTTCTCTCTGTCTTTGTATTTTCT 7 0.17500000000000002 No Hit GGCGGCTTTGCGAACAAAGTATCGATATGCTGCGACCTTCTTTTTCCTGG 6 0.15 No Hit CTAATTTTGCCACAGTTAGTGAAGAAATCCAAGGGGTGCACTTCCACACC 6 0.15 No Hit ATGGCTAATTAATACAGCTTATTGAATCTCAGCAAACTATTTATGAGGAT 6 0.15 No Hit CTCTGGCTTGCTGACATGGCGGTCGAGAATAATTTGATGAATTGTCGGGA 6 0.15 No Hit GTACGCATCTCCCGGGGGTGCGGCAACGATTATGGCAAGGCTGACCACAT 6 0.15 No Hit GGGTGAAGTTAGATTGGGTTCAGAAGAAGTTTTCCTTGGTGGTTTAAGGG 6 0.15 No Hit GTAAAATCAAAATGGCTTCTGTAAATACATGAACATGTCCTGCAAAGGTG 6 0.15 No Hit GTAGGAACCAGCTGCTTTCTCTTTCTCCTTCTCCTTCTCTTCCTCGGTCT 6 0.15 No Hit ATCTCGTAGTATGCCTTTGCCTGAGGTCTCTTAATCTTAAATGTTGGGGT 6 0.15 No Hit AGCATTTTCCGGTGGCGGAACTCGGTAGACTTGGTCTTTAGGGATCTGGA 6 0.15 No Hit CTCTTCTTCTAGATCATCACTGTCTTCCCGCTGATGATTTTTTCTTCCCC 6 0.15 No Hit TGGATTTGTTGAAGAAAGCAGTGGTGTTAAAGAAGGTGGATTGAGAAGAA 6 0.15 No Hit CGTCTTGATTGGTTGTCTTATTTAGGCTCCGACGGTTGTAGGTGAGAGAA 6 0.15 No Hit GTCGAGGCATAGCCAGCAAAAATGCCCATGAAAACCCAAAGTAAGAGCAT 6 0.15 No Hit CCACGTACCAAACATATGAATTACAACTAGTCCACGATTATATATCAATA 6 0.15 No Hit AGCAACTATACATAGGGTGCCATATACATATTACCATAATCTGTTGAGCC 6 0.15 No Hit CAGGACTGGTTTGTCGAGGGTGTGGCGGCTGACTACGACGACTGGACTGG 6 0.15 No Hit CTTCTCAGGAACAGAGCTGCTCCCACTCTCAGCTTCTTTTATCTGCCTCA 6 0.15 No Hit CCGATTAACCAGAACTGCTCGTTCCTCCACCACTCTTCCAGTGTAATTCC 6 0.15 No Hit GGAGTCCTTTACATGTGATAACAGTGAAAACGACATTATGGCCGCTCCCT 6 0.15 No Hit CTCCCCAGGTAATATCAAAATCGTTGTAGAAGTTGCTTGCTGAAGCAACC 6 0.15 No Hit GGGTACTACACGAAACTGATATCTTTATGGAAGCAACAGAATCTACTAGA 5 0.125 No Hit GTCATTGGCCTTAATGAGGGGATCTGGGTAGCGGATGGTGCGCCCATCAT 5 0.125 No Hit GCCTTAGCTCTTTCCTGCTGAGTTGATTGTCGTGATTCTTGTCATGTCTC 5 0.125 No Hit AGCAACGAGACAACTAAAAGAGCACACCAAGTCAGGTGTGTGCATCCGAG 5 0.125 No Hit CTTCGGCTGAAACCGTATACTCTTGCTTCTTGCCTGCTTCAGTCGAGAGT 5 0.125 No Hit ATCCATGGCAACATCATGGAACTCAAATGGCACATTGCATTCCTCTGCGA 5 0.125 No Hit CGCCACTTTATTTAGAAGAGCAAAGATTGCTTGTGAGAACATACCTTATC 5 0.125 No Hit CTGCAATTGCCCTTGCAACATCTAGCATTTTCCATGCCAAATCAAGGTCA 5 0.125 No Hit TGATGCAGCAAAAAAAATAATGCTGGATATAACATAAAAATCCAGCCAGT 5 0.125 No Hit CCAAAAACTCTTAACTTGCAGGGCCATACCTTCTGGTAAAGTAGAAGCCT 5 0.125 No Hit GTCAAGTCTACTAATTTTGCTAGTTTGTCCTTTTTGTCATCAGAGGATCT 5 0.125 No Hit GTACAGCGGTGCGTGCAATTGTCAGGCACGACCAGAGCGTCCGGTTCCTC 5 0.125 No Hit GGGTTGAATGAATTTGTCCTCGCTAGCATGCCCAAATAGAGCAGGAATGA 5 0.125 No Hit CCTGCTTTAGTTCCTGGATAGTGAACTTTCTATAATCTTCCTGGTCCGAT 5 0.125 No Hit CTCTCGTTCAGTTGTCCCTCTGCTTCAGGATCATCCAACTCACTGTTGCT 5 0.125 No Hit GCTTCTCGGCCTCGGCTTCACTCAATCGTTGTTTTAAGTAACTGCTAGCA 5 0.125 No Hit AGCTCATTTTTGTTGACTATCCACTCACCATCAACAATGTACTTGTACTC 5 0.125 No Hit TTTCACTATAGAAGCTTGTTTTCTAGTCTTCTTGTACACATCATCCACCA 5 0.125 No Hit ATGGCGGTTTGGGCGGCGTTCATGAGCTTGGTAGCGAGGCCAAGCTTACG 5 0.125 No Hit GCCTTGATGTCTAGTCTCTCTTTCTTTGGGCCCACTAATACACTCGTGTC 5 0.125 No Hit GCAACCAATTTGAATAATCTCCCAACTTATACTCCTTATACCCCTTCCCG 5 0.125 No Hit CCAAAATCAGCCCCACCGAATACGTGGAGCCCACCTCCTTCAGAAACTGG 5 0.125 No Hit GCATGGAAGCGTCAAGTGTCCTGTATGGACCTTCATGGGATAATTCTAGG 5 0.125 No Hit GCTTCCAAGTCTTGAACTTTCATCAACTTCAAGCAACTGGGTGATGAGCT 5 0.125 No Hit GATAGGGAAGTCCTCTTGGCACGGTACGTGATGGAAGCCTAATGTGTACC 5 0.125 No Hit GGCCCGGAAATTCAGCTTACAAGCACTTGTAAAATCATGGTGTCCTTGTT 5 0.125 No Hit GCCAAACCTTCTCTGTCTGGTGCACTTAACTCGGTAAAACCACAGCAATA 5 0.125 No Hit GTTCTACATGACTGCAAATGGTACCACATCACGGTACTCGTAGCACAAGG 5 0.125 No Hit GTGCGGGATGTGCGATCAAACTGACGAGTCATAGAAAACAGGCTGCTGGC 5 0.125 No Hit CCAGTTCTTTGCCCTCCAAGATATATCCATCAGCTCGCCCACACTGACCA 5 0.125 No Hit ATCCGTTTCTCAATAGAGATTCTATCAAACATGAAGGCATATTAGTTTGT 5 0.125 No Hit CCCAGGAAGTCTTCTCGGTAGACTTGCCAACCTCCTCCCCTGTTTTCTTC 5 0.125 No Hit GTCCTCCTAAATTTTGTTAACTGTACAATAACCTTAGCATCAGCTTTCAA 5 0.125 No Hit CCTGGGGCACATCTGAAGCATTAGCTTGAGATTGTAATTGCAGTCTTTCT 5 0.125 No Hit CCATGATCATCATCAGAACTTGATCTTGCACTTGTTGTTACTCTCAGCAA 5 0.125 No Hit GCGAAGTACCGAATCTCCTCAGAAGCAAATATCCAACATGCAACAACAGC 5 0.125 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0 0.0 0.0 0.0 0.0 64-65 0.0 0.0 0.0 0.0 0.0 66-67 0.0 0.0 0.0 0.0 0.0 68-69 0.0 0.0 0.0 0.0 0.0 70-71 0.0 0.0 0.0 0.0 0.0 72-73 0.0 0.0 0.0 0.0 0.0 74-75 0.0 0.0 0.0 0.0 0.0 76-77 0.0 0.0 0.0 0.0 0.0 78-79 0.0 0.0 0.0 0.0 0.0 80-81 0.0 0.0 0.0 0.0 0.0 82-83 0.037500000000000006 0.0 0.0 0.0 0.0 84-85 0.0625 0.0 0.0 0.0 0.0 86-87 0.15 0.0 0.0 0.0 0.0 88-89 0.3875 0.0 0.0 0.0 0.0 90-91 0.55 0.0 0.0 0.0 0.0 92-93 0.825 0.0 0.0 0.0 0.0 94-95 0.9 0.0 0.0 0.0 0.0 96-97 0.9375 0.0 0.0 0.0 0.0 98-99 1.1125 0.0 0.0 0.0 0.0 100-101 1.3375 0.0 0.0 0.0 0.0 102-103 1.625 0.0 0.0 0.0 0.0 104-105 2.075 0.0 0.0 0.0 0.0 106-107 2.4625 0.0 0.0 0.0 0.0 108-109 2.7375 0.0 0.0 0.0 0.0 110-111 3.15 0.0 0.0 0.0 0.0 112-113 3.475 0.0 0.0 0.0 0.0 114-115 3.8125 0.0 0.0 0.0 0.0 116-117 4.25 0.0 0.0 0.0 0.0 118-119 4.675 0.0 0.0 0.0 0.0 120-121 5.074999999999999 0.0 0.0 0.0 0.0 122-123 5.3875 0.0 0.0 0.0 0.0 124-125 6.0125 0.0 0.0 0.0 0.0 126-127 6.6 0.0 0.0 0.0 0.0 128-129 7.225 0.0 0.0 0.0 0.0 130-131 7.6375 0.0 0.0 0.0 0.0 132-133 8.350000000000001 0.0 0.0 0.0 0.0 134-135 9.3125 0.0 0.0 0.0 0.0 136-137 9.8875 0.0 0.0 0.0 0.0 138-139 10.6125 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TGCATCC 10 0.006830828 145.0 7 TAAAATA 10 0.006830828 145.0 7 ATGGCTA 10 0.006830828 145.0 1 CATCAGT 10 0.006830828 145.0 1 GCTAATT 10 0.006830828 145.0 4 CTAATTA 10 0.006830828 145.0 5 GGCTAAT 10 0.006830828 145.0 3 AAAAAAT 10 0.006830828 145.0 6 TGGCTAA 10 0.006830828 145.0 2 >>END_MODULE SRR26075382 read2 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR26075382_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.416 37.0 37.0 37.0 37.0 37.0 2 36.2405 37.0 37.0 37.0 37.0 37.0 3 36.352 37.0 37.0 37.0 37.0 37.0 4 36.4595 37.0 37.0 37.0 37.0 37.0 5 36.3355 37.0 37.0 37.0 37.0 37.0 6 36.3685 37.0 37.0 37.0 37.0 37.0 7 36.2845 37.0 37.0 37.0 37.0 37.0 8 36.438 37.0 37.0 37.0 37.0 37.0 9 36.492 37.0 37.0 37.0 37.0 37.0 10-14 36.3141 37.0 37.0 37.0 37.0 37.0 15-19 36.25699999999999 37.0 37.0 37.0 37.0 37.0 20-24 36.2014 37.0 37.0 37.0 37.0 37.0 25-29 36.1028 37.0 37.0 37.0 37.0 37.0 30-34 36.013400000000004 37.0 37.0 37.0 37.0 37.0 35-39 35.9567 37.0 37.0 37.0 37.0 37.0 40-44 35.8794 37.0 37.0 37.0 37.0 37.0 45-49 35.8017 37.0 37.0 37.0 37.0 37.0 50-54 35.74210000000001 37.0 37.0 37.0 37.0 37.0 55-59 35.7476 37.0 37.0 37.0 37.0 37.0 60-64 35.7347 37.0 37.0 37.0 37.0 37.0 65-69 35.7269 37.0 37.0 37.0 37.0 37.0 70-74 35.6125 37.0 37.0 37.0 37.0 37.0 75-79 35.506899999999995 37.0 37.0 37.0 37.0 37.0 80-84 35.5864 37.0 37.0 37.0 37.0 37.0 85-89 35.5619 37.0 37.0 37.0 37.0 37.0 90-94 35.514700000000005 37.0 37.0 37.0 37.0 37.0 95-99 35.544200000000004 37.0 37.0 37.0 37.0 37.0 100-104 35.403999999999996 37.0 37.0 37.0 37.0 37.0 105-109 35.3941 37.0 37.0 37.0 37.0 37.0 110-114 35.2635 37.0 37.0 37.0 34.6 37.0 115-119 35.301 37.0 37.0 37.0 37.0 37.0 120-124 35.169 37.0 37.0 37.0 32.2 37.0 125-129 35.2111 37.0 37.0 37.0 34.6 37.0 130-134 35.1505 37.0 37.0 37.0 29.8 37.0 135-139 35.0125 37.0 37.0 37.0 29.8 37.0 140-144 34.9533 37.0 37.0 37.0 25.0 37.0 145-149 34.9644 37.0 37.0 37.0 25.0 37.0 150-151 34.6495 37.0 37.0 37.0 25.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.0 1101 2 0.0 1101 3 0.0 1101 4 0.0 1101 5 0.0 1101 6 0.0 1101 7 0.0 1101 8 0.0 1101 9 0.0 1101 10-14 0.0 1101 15-19 0.0 1101 20-24 0.0 1101 25-29 0.0 1101 30-34 0.0 1101 35-39 0.0 1101 40-44 0.0 1101 45-49 0.0 1101 50-54 0.0 1101 55-59 0.0 1101 60-64 0.0 1101 65-69 0.0 1101 70-74 0.0 1101 75-79 0.0 1101 80-84 0.0 1101 85-89 0.0 1101 90-94 0.0 1101 95-99 0.0 1101 100-104 0.0 1101 105-109 0.0 1101 110-114 0.0 1101 115-119 0.0 1101 120-124 0.0 1101 125-129 0.0 1101 130-134 0.0 1101 135-139 0.0 1101 140-144 0.0 1101 145-149 0.0 1101 150-151 0.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 12 1.0 13 4.0 14 11.0 15 4.0 16 8.0 17 3.0 18 4.0 19 5.0 20 3.0 21 6.0 22 11.0 23 8.0 24 10.0 25 19.0 26 17.0 27 11.0 28 22.0 29 24.0 30 18.0 31 33.0 32 42.0 33 90.0 34 161.0 35 623.0 36 2645.0 37 217.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.0 24.4 9.225 22.375 2 29.049999999999997 27.3 25.674999999999997 17.974999999999998 3 24.0 29.725 28.599999999999998 17.675 4 24.775 33.7 22.650000000000002 18.875 5 25.85 35.15 21.025 17.974999999999998 6 23.25 38.800000000000004 19.275000000000002 18.675 7 21.975 22.85 35.25 19.925 8 22.325 26.474999999999998 28.025 23.175 9 23.625 26.5 26.775 23.1 10-14 25.135 29.565 24.595 20.705000000000002 15-19 24.86 28.38 26.029999999999998 20.73 20-24 24.88 28.76 25.979999999999997 20.380000000000003 25-29 24.14 28.57 26.26 21.029999999999998 30-34 25.424999999999997 28.225 25.905 20.445 35-39 25.06 27.334999999999997 26.945000000000004 20.66 40-44 24.705 28.715000000000003 25.64 20.94 45-49 24.635 28.59 26.064999999999998 20.71 50-54 24.834999999999997 28.365000000000002 26.255 20.544999999999998 55-59 24.77 28.505000000000003 26.465 20.26 60-64 24.73 27.83 26.445 20.995 65-69 25.790000000000003 27.195000000000004 26.32 20.695 70-74 24.505 27.345000000000002 27.12 21.029999999999998 75-79 23.84 28.835 26.56 20.765 80-84 24.12 28.310000000000002 27.045 20.525 85-89 24.485 28.26 26.400000000000002 20.855 90-94 24.59 27.450000000000003 27.625 20.335 95-99 24.82 27.11 26.71 21.36 100-104 24.605 28.299999999999997 26.235000000000003 20.86 105-109 25.2 29.095 25.324999999999996 20.380000000000003 110-114 25.39 29.165000000000003 25.96 19.485 115-119 25.555 27.884999999999998 27.11 19.45 120-124 25.52 27.925 26.965 19.59 125-129 25.619999999999997 28.46 25.929999999999996 19.99 130-134 26.085 28.294999999999998 25.840000000000003 19.78 135-139 25.985000000000003 28.78 25.88 19.355 140-144 27.029999999999998 28.645 25.45 18.875 145-149 26.695 28.37 26.529999999999998 18.404999999999998 150-151 26.450000000000003 28.749999999999996 26.35 18.45 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.5 3 1.0 4 2.0 5 1.5 6 0.5 7 0.5 8 1.0 9 1.5 10 1.0 11 1.0 12 0.5 13 0.0 14 0.0 15 1.0 16 1.5 17 1.0 18 0.5 19 0.5 20 0.5 21 0.0 22 1.5 23 2.0 24 1.0 25 1.5 26 1.0 27 3.0 28 7.0 29 9.0 30 8.5 31 14.0 32 16.5 33 18.5 34 37.5 35 47.0 36 49.0 37 68.5 38 113.0 39 147.0 40 174.5 41 209.5 42 229.5 43 263.0 44 276.5 45 288.5 46 299.0 47 258.5 48 207.5 49 202.5 50 192.5 51 164.0 52 147.0 53 105.5 54 74.0 55 59.5 56 49.5 57 42.0 58 37.5 59 32.0 60 24.5 61 14.5 62 6.0 63 8.5 64 6.5 65 3.0 66 4.0 67 2.5 68 3.0 69 3.5 70 3.5 71 3.5 72 2.0 73 1.0 74 0.0 75 1.0 76 1.0 77 0.0 78 0.5 79 1.0 80 0.5 81 0.0 82 0.0 83 1.5 84 4.0 85 3.0 86 0.5 87 1.0 88 2.5 89 2.0 90 1.0 91 1.0 92 0.5 93 0.0 94 1.0 95 3.0 96 3.0 97 1.5 98 0.5 99 0.0 100 5.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150-151 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 63.224999999999994 #Duplication Level Percentage of deduplicated Percentage of total 1 65.75721629102412 41.575 2 21.62910241202056 27.35 3 6.919731119019374 13.125 4 2.926057730328193 7.3999999999999995 5 1.4234875444839856 4.5 6 0.7512850929221035 2.85 7 0.2372479240806643 1.05 8 0.11862396204033215 0.6 9 0.15816528272044286 0.8999999999999999 >10 0.07908264136022143 0.65 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG 15 0.375 No Hit GAGAATAGAGCGCTGTTCTGCTATTATAGAATAATGGATCAGGGAAGAGG 11 0.27499999999999997 No Hit GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGT 9 0.22499999999999998 No Hit CTTCAGCTTCATCAGGAATCTGCGACATGTGGTACTGCAGCCTGCAGAAA 9 0.22499999999999998 No Hit GAAACAGAGAGTAGTATGGAAAGTACTGGTTCTGTGATCACTAATAAGGT 9 0.22499999999999998 No Hit GGAGAAAGTTCGAGAAGCGAAGAAAGCTAATAGAGAGGCTAGGGAAGCCA 9 0.22499999999999998 No Hit GTGGATTTTGGGAGCACGAACCCTTTCTTACGCTGGGTTTCAAGCGTTTA 8 0.2 No Hit TGACCCTGAACACAAAAAAGAGTCCATGGAGAAGTATCTGGGTTCTTCAA 8 0.2 No Hit ACTTCCCAAAATGGCATCTGGGTCATCGGGTCGGACTAACTCGGGTTCAA 8 0.2 No Hit GAAAGAGAGAAACTTGGTGGGAAGCCTGTCAAGTATCATGGGAAATGGCT 7 0.17500000000000002 No Hit AGTTGACCGAATTGCGGAAATTGATCTAGTAGTGAATTTCAAATCCACTG 7 0.17500000000000002 No Hit GAAGGAGCTCGAATGCTAGGGGCAGCTAAGATAATCGGCGTTGATAAGAA 7 0.17500000000000002 No Hit GGCAGGAGAGGTTGAAATGGGTCCGGGAGAATTACATGGTATACAATTAC 7 0.17500000000000002 No Hit CAAGGAAGAAGAGAAAAAAGATGAGCCTCCTCCCGTTGTAACTGTAGTCT 7 0.17500000000000002 No Hit GTTATACTTATGTTTTGTTTTTGTGTGATTTATGTCTCAAACAAGGCTAG 7 0.17500000000000002 No Hit GAGGATATTCCGGTTGCGTCTCCACCACCTCCAGAGTATGAACATACTGA 6 0.15 No Hit GTTTTGAATTTGTAAAAGCTGTGACACTGGTGCTAGAACAATTTACAGTG 6 0.15 No Hit CATAGGTTGAAGCTGCTGCAGAGAATTGCTTACCTCAATGTTGGAATTTA 6 0.15 No Hit AAAACTTTACTTGTATATCATCCTTGGCATCAATCGTTTCATAATAGCCG 6 0.15 No Hit GGGAAAGGTTGTTAAAGCAATCAGTGAAGTGGAAGAGAAAGATGGGAAAC 6 0.15 No Hit GGAAAGACCACATTCGTTAAGAGGCATCTTACCGGAGAGTTCGAGAAGAA 6 0.15 No Hit ACGGTGACGAGCAAAGAGGCGGATTATGTATTGGAGCATATCCACAACCT 6 0.15 No Hit GCTACACCATTGCGGTGGAGAAGGCAAGGAAGATTGCACCATCAGAAGAA 6 0.15 No Hit ACCAGTGGAAGAAGAGAAGAAGCAGAAAGATAAGTTAAAAGAAGGTAAGG 6 0.15 No Hit GGATTATTAGCTGGGTTATTGAGCTCCTTAGTGGCTGGAGCCGCCGTGGC 6 0.15 No Hit CTTCATCTTCTCTTTCATTCCCATATCGCCTCCTCCCAAAAACTAACTTC 6 0.15 No Hit TCTAATTCAGATCTGCTGTGTGTCTATGTTGGAACTGGCGTTCAGTTTTT 6 0.15 No Hit CAGCAGCCAAAACTCTCCTCAAGACCCAGAAGTCCAAAAAGGGAAAAAAA 6 0.15 No Hit AGGAGGTGAAGCAGAGCAATCATGTCCAGAGGAAGCTGGAGAAGAGACAA 6 0.15 No Hit GGAAGAAATCTGACTGTGATAGCAGAGGAGTTCAAGCTAAAGACAACTGG 6 0.15 No Hit AATACCTTCAACTATGGTCAGAAATAATCAGTAATGAATAATCATGAAGG 6 0.15 No Hit CAAGCTATTAAATTATTTGATCAACCAACTAAATACAACATAAACTTACT 6 0.15 No Hit CACTGACTCTGTCATCGTCGACAGCAACAATGGCCGACCGAGTTTATCCT 6 0.15 No Hit TGATTTCTTACCATCTGCCTTATTAAACTCTTCATTACACTCATTCACAG 6 0.15 No Hit GAAGACAACAATTATTCTACAGTGGAAATTTCTGGGCTTGACATTGGATG 5 0.125 No Hit ATCTCCTCTATGTCGCTGAAGATTACAACGGCCGAATTGTCGGTTACGTT 5 0.125 No Hit TCAGAGAAGAAATAGGAGGATGCGAGGAGCCAAACATGGAAGGAGAAGAC 5 0.125 No Hit ATAAGGCCAAGGAGATCATGGCTTTGGCTGCCAAGGGAAGCTCTATTAGC 5 0.125 No Hit ACAACCTGTTACCCTTCAAGGTGAAGATTTGAAGCAATTTAACAGCGAAA 5 0.125 No Hit TGTGGACTGAGGTGCCAAAAACTGGGAAAGGCAAGAAGAAAGCTCAGCCA 5 0.125 No Hit GAGTGTTCTAATGCAGATGCTTGCTTATCGGCCAGGTGGGCCCCCAGTAA 5 0.125 No Hit GTCACTAGCCTTCTTTATGGAGCAGAAGATCCATCTATAGCTGGGATGGT 5 0.125 No Hit ACGGCCGCCACGACTTCAACTGCGACTTCTCTGGAAGTGGACAGATCACC 5 0.125 No Hit GCCTCGCTCCTGGGTGACGCCATCGCTTACATCAACGAGCTCAAGACGAA 5 0.125 No Hit TCTCTGGCTTGATCAGCAGGGCGATCAACGGCAGAGGATTCTCAGCTGAT 5 0.125 No Hit GGATAGGAAAGAGCTGAAGTTGAGGGTTACTGGTCGTGTATGGAAAGAAG 5 0.125 No Hit CAGCAAAAAAATCAGCAGCAGCAAAATAGCAAGGCAAACCATGATGCCAA 5 0.125 No Hit GAACAATGTTTCGTTTAAGCAACAACTTGGTAGGAATCCTGAATTTCATA 5 0.125 No Hit AAATAATCTCAAACTGTGATGGGTCTTCTCGTTCTTTGGATCACCCAGCT 5 0.125 No Hit GCTACGTTGATTTGATACATGGAACTTGGAGAATTCAGGGCTGCTTGGCA 5 0.125 No Hit GGTTGGAAACCCCGTTGGGTACAAGTTGGTTCCCGGTGGCACGGCTGCTA 5 0.125 No Hit GCTGTAGAAACCACTGTCAATCTGGGTCCAATTGCAACTGCTTAATCTTC 5 0.125 No Hit GAGTGGTTTGGCTGGTGGTGCTGCGAAAAATCTGGATCTTGATGATGCAG 5 0.125 No Hit AAACTGATCCTTTAGCGGAGATGAGTTACTACAATCAGCAGCAGGCTCCA 5 0.125 No Hit TGAGATCCAAGTATCATGCTTTGCTGCAAGTGCTGTCGATATTCCTAGTT 5 0.125 No Hit AGGCCGTGAAGTGCTTGCTATTTTGATGCAGAGACATGTCCTTGTTGATG 5 0.125 No Hit GTTTCACTTGGAGTAGCGGTATTGATAGCAGCAACAGCAATCCTCCTGGT 5 0.125 No Hit AGAATTACCACATATTTTTCGAAGCTGTGCTCGTGGTAGTCCAAAACGAC 5 0.125 No Hit GGTAGCTAAAGAGGATGTGAATTACATTAATGCACATGCTACATCCACAC 5 0.125 No Hit TGAACGGAAAGCCCACAGTGTTGCAAATAAAAGAGCTGAAGACTTCTCTC 5 0.125 No Hit TGATGAGGGAAGTGATGATGATGACGACCTTGCTGATGCAGATGAAGAGG 5 0.125 No Hit GATCACAGCCCTGCCACCCCACAGAGGCCTGATGGTTCTTCTTCGTCACA 5 0.125 No Hit GTGATATGATGCGACAAAAGTTATTGCAGCCACCAACATCAGTAGGTTTT 5 0.125 No Hit CTTGCAGTTGGTTATGGAGTAGAGAATGGTATCCCATATTGGCTGATCAA 5 0.125 No Hit TTTTTTAAACACAAAGTAATATTTCCAAGATGAAGGCCTTTCTTATCATA 5 0.125 No Hit AAGTTGAATCCTGTTACAATCATTTTCTATCGTTTTTCCCGCTTTATTAT 5 0.125 No Hit CAATATCTTATAAACACCAACTAAAATTATCCGCTACCTGAACCCTTTCC 5 0.125 No Hit ATTAACGTTCACTATGCCTGGCTGCACCACCTGTTCTTGCTAGACTTCCG 5 0.125 No Hit ATTTCATGCTGCAAGGTGAAATGCCATTTGGGTCGTGGAGAGACAGTGAA 5 0.125 No Hit GGAAATTATGGGGACTGGTACAACAGTCGCGGCGCTGTTCTTCCGCCTCG 5 0.125 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0 0.0 0.0 0.0 0.0 64-65 0.0 0.0 0.0 0.0 0.0 66-67 0.0 0.0 0.0 0.0 0.0 68-69 0.0 0.0 0.0 0.0 0.0 70-71 0.0 0.0 0.0 0.0 0.0 72-73 0.0 0.0 0.0 0.0 0.0 74-75 0.0 0.0 0.0 0.0 0.0 76-77 0.0 0.0 0.0 0.0 0.0 78-79 0.0 0.0 0.0 0.0 0.0 80-81 0.0 0.0 0.0 0.0 0.0 82-83 0.037500000000000006 0.0 0.0 0.0 0.0 84-85 0.0625 0.0 0.0 0.0 0.0 86-87 0.15 0.0 0.0 0.0 0.0 88-89 0.3875 0.0 0.0 0.0 0.0 90-91 0.55 0.0 0.0 0.0 0.0 92-93 0.825 0.0 0.0 0.0 0.0 94-95 0.9 0.0 0.0 0.0 0.0 96-97 0.9375 0.0 0.0 0.0 0.0 98-99 1.1125 0.0 0.0 0.0 0.0 100-101 1.3375 0.0 0.0 0.0 0.0 102-103 1.65 0.0 0.0 0.0 0.0 104-105 2.0999999999999996 0.0 0.0 0.0 0.0 106-107 2.5 0.0 0.0 0.0 0.0 108-109 2.7875 0.0 0.0 0.0 0.0 110-111 3.1875 0.0 0.0 0.0 0.0 112-113 3.5 0.0 0.0 0.0 0.0 114-115 3.8499999999999996 0.0 0.0 0.0 0.0 116-117 4.324999999999999 0.0 0.0 0.0 0.0 118-119 4.75 0.0 0.0 0.0 0.0 120-121 5.15 0.0 0.0 0.0 0.0 122-123 5.4875 0.0 0.0 0.0 0.0 124-125 6.0875 0.0 0.0 0.0 0.0 126-127 6.65 0.0 0.0 0.0 0.0 128-129 7.25 0.0 0.0 0.0 0.0 130-131 7.6875 0.0 0.0 0.0 0.0 132-133 8.375 0.0 0.0 0.0 0.0 134-135 9.3125 0.0 0.0 0.0 0.0 136-137 9.8875 0.0 0.0 0.0 0.0 138-139 10.6125 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTTGTT 10 0.006830828 145.0 7 TGCAAAA 10 0.006830828 145.0 2 GCAAAAG 10 0.006830828 145.0 3 ATTGCAG 10 0.006830828 145.0 1 AAAGGTT 10 0.006830828 145.0 4 AAGGTTG 10 0.006830828 145.0 5 AGGTTGT 10 0.006830828 145.0 6 GTTGTTA 10 0.006830828 145.0 8 GGAAAGG 10 0.006830828 145.0 2 GGAAAGA 40 0.0076550315 18.125 140-144 >>END_MODULE Read 1426127 spots for SRR26075382.sra Written 1426127 spots for SRR26075382.sra Read 1426127 spots for SRR26075382.sra Written 1426127 spots for SRR26075382.sra Read 1426127 spots for SRR26075382.sra Written 1426127 spots for SRR26075382.sra Read 1426127 spots for SRR26075382.sra Written 1426127 spots for SRR26075382.sra Read 1426127 spots for SRR26075382.sra Written 1426127 spots for SRR26075382.sra Read 1426127 spots for SRR26075382.sra Written 1426127 spots for SRR26075382.sra Read 1426127 spots for SRR26075382.sra Written 1426127 spots for SRR26075382.sra Read 1426127 spots for SRR26075382.sra Written 1426127 spots for SRR26075382.sra Read 1426133 spots for SRR26075382.sra Written 1426133 spots for SRR26075382.sra Read 1426127 spots for SRR26075382.sra Written 1426127 spots for SRR26075382.sra Read 1426127 spots for SRR26075382.sra Written 1426127 spots for SRR26075382.sra Read 1426127 spots for SRR26075382.sra Written 1426127 spots for SRR26075382.sra Read 1426127 spots for SRR26075382.sra Written 1426127 spots for SRR26075382.sra Read 1426127 spots for SRR26075382.sra Written 1426127 spots for SRR26075382.sra Read 1426127 spots for SRR26075382.sra Written 1426127 spots for SRR26075382.sra Read 1426127 spots for SRR26075382.sra Written 1426127 spots for SRR26075382.sra Read 1426127 spots for SRR26075382.sra Written 1426127 spots for SRR26075382.sra Read 1426127 spots for SRR26075382.sra Written 1426127 spots for SRR26075382.sra Read 1426127 spots for SRR26075382.sra Written 1426127 spots for SRR26075382.sra Read 1426127 spots for SRR26075382.sra Written 1426127 spots for SRR26075382.sra SRR ids: ['SRR26075382.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_m3f901qu SRR26075382.sra spots: 28522546 blocks: [[1, 1426127], [1426128, 2852254], [2852255, 4278381], [4278382, 5704508], [5704509, 7130635], [7130636, 8556762], [8556763, 9982889], [9982890, 11409016], [11409017, 12835143], [12835144, 14261270], [14261271, 15687397], [15687398, 17113524], [17113525, 18539651], [18539652, 19965778], [19965779, 21391905], [21391906, 22818032], [22818033, 24244159], [24244160, 25670286], [25670287, 27096413], [27096414, 28522546]] SRR26075382 file size 10530948 SRR26075382 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o SRR26075382 SRR26075382_1.fastq SRR26075382_2.fastq Input file: SRR26075382_1.fastq Paired file: SRR26075382_2.fastq trimmed: SRR26075382-trimmed-pair1.fastq, SRR26075382-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Tue Feb 11 23:54:44 2025 >> started Tue Feb 11 23:55:17 2025 >> done (32.875s) 28522546 read pairs processed; of these: 132 ( 0.00%) short read pairs filtered out after trimming by size control 108936 ( 0.38%) empty read pairs filtered out after trimming by size control 28413478 (99.62%) read pairs available; of these: 4297315 (15.12%) trimmed read pairs available after processing 24116163 (84.88%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 18 8 0.00% 19 15 0.00% 20 12 0.00% 21 22 0.00% 22 15 0.00% 23 28 0.00% 24 19 0.00% 25 31 0.00% 26 29 0.00% 27 39 0.00% 28 27 0.00% 29 32 0.00% 30 30 0.00% 31 41 0.00% 32 35 0.00% 33 48 0.00% 34 44 0.00% 35 31 0.00% 36 55 0.00% 37 49 0.00% 38 72 0.00% 39 43 0.00% 40 50 0.00% 41 90 0.00% 42 58 0.00% 43 72 0.00% 44 81 0.00% 45 83 0.00% 46 124 0.00% 47 98 0.00% 48 123 0.00% 49 143 0.00% 50 156 0.00% 51 208 0.00% 52 206 0.00% 53 223 0.00% 54 256 0.00% 55 280 0.00% 56 349 0.00% 57 370 0.00% 58 402 0.00% 59 493 0.00% 60 534 0.00% 61 670 0.00% 62 680 0.00% 63 839 0.00% 64 906 0.00% 65 1086 0.00% 66 1215 0.00% 67 1265 0.00% 68 1475 0.01% 69 1713 0.01% 70 2016 0.01% 71 2407 0.01% 72 2736 0.01% 73 3111 0.01% 74 3543 0.01% 75 3941 0.01% 76 4449 0.02% 77 4763 0.02% 78 5344 0.02% 79 5960 0.02% 80 6679 0.02% 81 7472 0.03% 82 8393 0.03% 83 9509 0.03% 84 10809 0.04% 85 11993 0.04% 86 12954 0.05% 87 13946 0.05% 88 14462 0.05% 89 15834 0.06% 90 16914 0.06% 91 18442 0.06% 92 19940 0.07% 93 21866 0.08% 94 23592 0.08% 95 25177 0.09% 96 26816 0.09% 97 28251 0.10% 98 29297 0.10% 99 30790 0.11% 100 31766 0.11% 101 33197 0.12% 102 35206 0.12% 103 37730 0.13% 104 39405 0.14% 105 41491 0.15% 106 43345 0.15% 107 45057 0.16% 108 46515 0.16% 109 47901 0.17% 110 48869 0.17% 111 50606 0.18% 112 51846 0.18% 113 53629 0.19% 114 56735 0.20% 115 58965 0.21% 116 61644 0.22% 117 63080 0.22% 118 65462 0.23% 119 66050 0.23% 120 66670 0.23% 121 68421 0.24% 122 69712 0.25% 123 72395 0.25% 124 74422 0.26% 125 76441 0.27% 126 79457 0.28% 127 81768 0.29% 128 82753 0.29% 129 84453 0.30% 130 86972 0.31% 131 86598 0.30% 132 88374 0.31% 133 90543 0.32% 134 91856 0.32% 135 95105 0.33% 136 96211 0.34% 137 96839 0.34% 138 100988 0.36% 139 102521 0.36% 140 102264 0.36% 141 104608 0.37% 142 104963 0.37% 143 107525 0.38% 144 109110 0.38% 145 110375 0.39% 146 112947 0.40% 147 113934 0.40% 148 117095 0.41% 149 117396 0.41% 150 119721 0.42% 151 24116163 84.88% 28413478 reads passed initial QC criterion=sequence-density sequence-density=0.21 sequence-density-rank=1 fanout-score=5.87 fanout-score-rank=22 prefix-density=0.36 prefix-fanout=3.4 sequence=TCCTTGTCCTGGATCTT criterion=fanout-score sequence-density=0.07 sequence-density-rank=35 fanout-score=240.10 fanout-score-rank=1 prefix-density=0.62 prefix-fanout=27.3 sequence=ATCTTCTTCTTGTC criterion=sequence-density sequence-density=0.21 sequence-density-rank=1 fanout-score=5.28 fanout-score-rank=22 prefix-density=0.48 prefix-fanout=2.3 sequence=CAGAAGGAGTCCACCCTCCACTTGGTGCTTCG criterion=fanout-score sequence-density=0.09 sequence-density-rank=21 fanout-score=333.24 fanout-score-rank=1 prefix-density=1.02 prefix-fanout=30.4 sequence=AAGAAGAAGAAA SRR26075382 testing PE reads STAR mapping to Ensembl genome Started job on | Feb 11 23:56:52 Started mapping on | Feb 11 23:56:52 Finished on | Feb 12 00:07:01 Mapping speed, Million of reads per hour | 167.96 Number of input reads | 28413478 Average input read length | 290 UNIQUE READS: Uniquely mapped reads number | 18731194 Uniquely mapped reads % | 65.92% Average mapped length | 291.41 Number of splices: Total | 15660993 Number of splices: Annotated (sjdb) | 15241805 Number of splices: GT/AG | 15390960 Number of splices: GC/AG | 195314 Number of splices: AT/AC | 16148 Number of splices: Non-canonical | 58571 Mismatch rate per base, % | 0.44% Deletion rate per base | 0.03% Deletion average length | 3.16 Insertion rate per base | 0.02% Insertion average length | 2.57 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 620498 % of reads mapped to multiple loci | 2.18% Number of reads mapped to too many loci | 52548 % of reads mapped to too many loci | 0.18% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 31.28% % of reads unmapped: other | 0.42% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 9061786 9061786 9061786 N_multimapping 620498 620498 620498 N_noFeature 493952 18517349 610980 N_ambiguous 363050 2941 264522 UnstrandedReadsAssigned:17874192 PositiveStrandReadsAssigned:210904 NegativeStrandReadsAssigned:17855692 Dataset is classified negative stranded MeadianReadLen=151 20thPercentileLength=151 echo kmer=147 SRR26075382 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,400 [index] number of k-mers: 62,057,036 [index] number of equivalence classes: 130,681 [quant] running in paired-end mode [quant] will process pair 1: SRR26075382-trimmed-pair1.fastq SRR26075382-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 28,413,478 reads, 20,830,123 reads pseudoaligned [quant] estimated average fragment length: 209.656 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,180 rounds 52401 SRR26075382.ke.tsv 34699 SRR26075382.se.tsv 87100 total ==> SRR26075382.ke.tsv <== target_id length eff_length est_counts tpm Potri.005G200100.1.v4.1 2018 1809.34 2703 58.8734 Potri.005G024800.1.v4.1 1035 826.344 5534 263.92 Potri.004G059700.1.v4.1 961 752.354 6 0.314285 Potri.007G009000.2.v4.1 1416 1207.34 0 0 Potri.003G141000.2.v4.1 2943 2734.34 804 11.5877 Potri.016G087400.1.v4.1 270 91.6593 1910.41 821.382 Potri.015G069301.1.v4.1 564 356.958 0 0 Potri.010G195200.1.v4.1 1773 1564.34 70 1.76344 Potri.012G127500.1.v4.1 977 768.344 6719 344.622 ==> SRR26075382.se.tsv <== Potri.001G166300.v4.1 0 Potri.001G448400.v4.1 52 Potri.001G233950.v4.1 0 Potri.001G122700.v4.1 184 Potri.001G212900.v4.1 0 Potri.001G182400.v4.1 0 Potri.001G256600.v4.1 0 Potri.001G040500.v4.1 12 Potri.001G416900.v4.1 0 Potri.001G452600.v4.1 469 SRR26075382 completed mapping pipeline successfully