Starting /dee2/code/volunteer_pipeline.sh SRR26075383 current disk space = 3048854188032 free memory = 1305109180 SRR26075383 SRAfilesize 585061914e6787de94ca3d25f8846670 SRR26075383.sra SRR26075383.sra file validated SRR26075383 is paired end SRR26075383 is conventional basespace SRR26075383 read1 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR26075383_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.5935 37.0 37.0 37.0 37.0 37.0 2 36.636 37.0 37.0 37.0 37.0 37.0 3 36.735 37.0 37.0 37.0 37.0 37.0 4 36.705 37.0 37.0 37.0 37.0 37.0 5 36.673 37.0 37.0 37.0 37.0 37.0 6 36.678 37.0 37.0 37.0 37.0 37.0 7 36.641 37.0 37.0 37.0 37.0 37.0 8 36.7095 37.0 37.0 37.0 37.0 37.0 9 36.709 37.0 37.0 37.0 37.0 37.0 10-14 36.6501 37.0 37.0 37.0 37.0 37.0 15-19 36.6511 37.0 37.0 37.0 37.0 37.0 20-24 36.5741 37.0 37.0 37.0 37.0 37.0 25-29 36.5096 37.0 37.0 37.0 37.0 37.0 30-34 36.43470000000001 37.0 37.0 37.0 37.0 37.0 35-39 36.3785 37.0 37.0 37.0 37.0 37.0 40-44 36.371 37.0 37.0 37.0 37.0 37.0 45-49 36.284 37.0 37.0 37.0 37.0 37.0 50-54 36.1712 37.0 37.0 37.0 37.0 37.0 55-59 36.092 37.0 37.0 37.0 37.0 37.0 60-64 36.1263 37.0 37.0 37.0 37.0 37.0 65-69 36.028800000000004 37.0 37.0 37.0 37.0 37.0 70-74 36.0422 37.0 37.0 37.0 37.0 37.0 75-79 36.085699999999996 37.0 37.0 37.0 37.0 37.0 80-84 36.049499999999995 37.0 37.0 37.0 37.0 37.0 85-89 36.0048 37.0 37.0 37.0 37.0 37.0 90-94 35.9021 37.0 37.0 37.0 37.0 37.0 95-99 35.9028 37.0 37.0 37.0 37.0 37.0 100-104 35.8851 37.0 37.0 37.0 37.0 37.0 105-109 35.7767 37.0 37.0 37.0 37.0 37.0 110-114 35.7531 37.0 37.0 37.0 37.0 37.0 115-119 35.6499 37.0 37.0 37.0 37.0 37.0 120-124 35.67530000000001 37.0 37.0 37.0 37.0 37.0 125-129 35.5081 37.0 37.0 37.0 37.0 37.0 130-134 35.40859999999999 37.0 37.0 37.0 37.0 37.0 135-139 35.182 37.0 37.0 37.0 29.8 37.0 140-144 35.143299999999996 37.0 37.0 37.0 27.4 37.0 145-149 35.0835 37.0 37.0 37.0 27.4 37.0 150-151 34.85425 37.0 37.0 37.0 25.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.0 1101 2 0.0 1101 3 0.0 1101 4 0.0 1101 5 0.0 1101 6 0.0 1101 7 0.0 1101 8 0.0 1101 9 0.0 1101 10-14 0.0 1101 15-19 0.0 1101 20-24 0.0 1101 25-29 0.0 1101 30-34 0.0 1101 35-39 0.0 1101 40-44 0.0 1101 45-49 0.0 1101 50-54 0.0 1101 55-59 0.0 1101 60-64 0.0 1101 65-69 0.0 1101 70-74 0.0 1101 75-79 0.0 1101 80-84 0.0 1101 85-89 0.0 1101 90-94 0.0 1101 95-99 0.0 1101 100-104 0.0 1101 105-109 0.0 1101 110-114 0.0 1101 115-119 0.0 1101 120-124 0.0 1101 125-129 0.0 1101 130-134 0.0 1101 135-139 0.0 1101 140-144 0.0 1101 145-149 0.0 1101 150-151 0.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 20 1.0 21 3.0 22 8.0 23 1.0 24 3.0 25 5.0 26 10.0 27 13.0 28 15.0 29 23.0 30 26.0 31 45.0 32 70.0 33 108.0 34 163.0 35 413.0 36 2893.0 37 200.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 35.53884711779449 17.192982456140353 8.270676691729323 38.99749373433584 2 20.275000000000002 14.674999999999999 31.5 33.550000000000004 3 16.7 17.95 28.999999999999996 36.35 4 21.3 22.35 23.75 32.6 5 24.725 26.674999999999997 25.724999999999998 22.875 6 25.324999999999996 30.425 22.975 21.275 7 16.950000000000003 28.549999999999997 36.325 18.175 8 19.775000000000002 27.275 30.65 22.3 9 15.825 26.85 31.874999999999996 25.45 10-14 19.026902690269026 30.673067306730672 27.47274727472747 22.827282728272827 15-19 20.055 27.815 27.79 24.34 20-24 19.935 28.64 27.625 23.799999999999997 25-29 20.005 28.249999999999996 27.615000000000002 24.13 30-34 19.994999999999997 27.650000000000002 28.115000000000002 24.240000000000002 35-39 21.154999999999998 27.325 28.000000000000004 23.52 40-44 20.175 28.455000000000002 28.015 23.355 45-49 19.875 27.595 27.584999999999997 24.945 50-54 20.64 27.794999999999998 27.284999999999997 24.279999999999998 55-59 20.715 27.415 27.595 24.275 60-64 20.53 26.634999999999998 27.884999999999998 24.95 65-69 20.36 27.415 27.71 24.515 70-74 20.96 27.91 27.834999999999997 23.294999999999998 75-79 20.315 27.224999999999998 28.095 24.365000000000002 80-84 21.055 27.43 27.0 24.515 85-89 20.974999999999998 26.575 27.565 24.884999999999998 90-94 21.21 27.445000000000004 26.095000000000002 25.25 95-99 21.435000000000002 27.49 27.26 23.815 100-104 21.355 26.700000000000003 27.68 24.265 105-109 21.01 27.229999999999997 27.894999999999996 23.865 110-114 21.695 28.96 26.169999999999998 23.175 115-119 21.035 27.18 27.275 24.51 120-124 21.14 27.63 26.724999999999998 24.505 125-129 20.549999999999997 27.66 26.985 24.805 130-134 22.384999999999998 26.815 26.724999999999998 24.075 135-139 21.34 27.395000000000003 26.445 24.82 140-144 21.94 27.145000000000003 27.200000000000003 23.715 145-149 22.615 27.705000000000002 25.8 23.880000000000003 150-151 23.0625 27.4125 25.25 24.275 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.5 14 0.5 15 0.0 16 0.5 17 0.5 18 1.0 19 1.0 20 0.0 21 0.0 22 0.5 23 1.0 24 1.0 25 0.5 26 2.0 27 3.5 28 5.0 29 5.0 30 16.0 31 26.5 32 31.5 33 40.0 34 40.0 35 44.5 36 58.5 37 69.5 38 96.5 39 141.0 40 179.0 41 216.5 42 228.0 43 246.5 44 283.0 45 266.5 46 272.0 47 275.0 48 243.5 49 217.0 50 177.5 51 165.5 52 152.5 53 119.5 54 89.0 55 61.5 56 45.0 57 39.0 58 31.0 59 21.0 60 15.5 61 14.5 62 11.0 63 7.0 64 5.5 65 3.0 66 1.5 67 2.0 68 1.0 69 1.0 70 2.0 71 2.0 72 2.5 73 4.0 74 3.5 75 3.0 76 3.0 77 1.5 78 1.0 79 0.5 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.25 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.01 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150-151 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 62.125 #Duplication Level Percentage of deduplicated Percentage of total 1 65.55331991951711 40.725 2 19.839034205231385 24.65 3 8.209255533199194 15.299999999999999 4 3.460764587525151 8.6 5 1.6096579476861168 5.0 6 0.5633802816901409 2.1 7 0.44265593561368205 1.925 8 0.2012072434607646 1.0 9 0.08048289738430583 0.44999999999999996 >10 0.04024144869215292 0.25 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GCAAAGAAAACAGAGAACAGAAGGGTATCTTGAAACTAAGACGGCAAGGA 10 0.25 No Hit CTCTCTCTCAACAAAGGTTTAAGCCATGGCGGCCACCTGTTGTCCTCATC 9 0.22499999999999998 No Hit CATTAGGAATGTATTCATAAACGAGTAGAAGCTCTCGGCTGTGCCTCGAG 9 0.22499999999999998 No Hit GTGGACCGATAACGTAGACCAATTGTAAATATTGCTTCCCATTCCTTGAT 8 0.2 No Hit CTCCGGTTCTTTGGGCTTCTCTGGTTGCTTAGGCTTCTCAGGTTCTTTCG 8 0.2 No Hit CCTGGTTTATATCTGGCTGTTCTGTACTTCTGGAGGTGGCTTTTGACGTG 8 0.2 No Hit CCTGGTTAAACAATTAATGGTTCCTTTTGAGCCGATCTTAGCTCAAAACT 8 0.2 No Hit AGTGATGACACTGTGGCATGAGGGCTTTGGAGACTGTGCTGTCATCCAAA 8 0.2 No Hit CACCTGGATATTTCACTGCCAATTCTTCCAAACATTTCATAAGCACACCA 7 0.17500000000000002 No Hit CCCATTGCTCGCTCTGGTTATATGGAGTGACCCATATTTGGTTATTTGTA 7 0.17500000000000002 No Hit GTTCGGAGAGAGTTTTGTCTGAGAATGGATTGCATAAGGTAGAATTGGAT 7 0.17500000000000002 No Hit TACTGATTAATTCTTCAATCATATCAGGCATTTTATCACCCAACCCAAGA 7 0.17500000000000002 No Hit CTACTGGAAAAACTTTCCTTAATCTCTCAGCAGAAAGGGATGCTCTTTTA 7 0.17500000000000002 No Hit AGCCCGTCATCTCTGCAAGTCCCACAAATTGCCCACTGGTATTGACCGAG 7 0.17500000000000002 No Hit GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGATACCAATCTCGGGT 7 0.17500000000000002 TruSeq Adapter, Index 11 (97% over 39bp) CGAGAATATTGCAAACCTTAACTTCATCTCCCCCTCTGTCTCATACCTCT 7 0.17500000000000002 No Hit GCAAAGGCTTCAATTAGGAATTTGTATTGGTTCAGGTTGCATGGCTTTTT 7 0.17500000000000002 No Hit GGTGTAAGCAATAGTCGAGATACCAACTAAAGCAGCAAGAAAGCAAGAAA 7 0.17500000000000002 No Hit AGCCTTTGAGAGTAAATTTTCCAGGTATACTTCTCTTGGATTCGCTGCAG 7 0.17500000000000002 No Hit GTGGCTTCCATCCCAACTCATCTGGACAGCGAAAAGTAGCTTCATTGACA 6 0.15 No Hit CATTCAACCGATTGTTGTTTGAAGAGTAGTTGGATGATGAAGGCGAGCCC 6 0.15 No Hit GGTGACGCCATAGCATGGCAAGAACAAGATTGTAAACTAGAGGAATTGGC 6 0.15 No Hit CCTCTAACTTTCAGAACCAGCTTTCGAGCTCCGAAAATCCTTTTTCAATT 6 0.15 No Hit ACTGCACGATTTCATCCATGGCTGGGCGACCTGCTGGATTTTTGTTCAAG 6 0.15 No Hit GTTTATAATTCCAAACACAAGCCCACCTGAACTCAACATTGTTAGGTAAG 6 0.15 No Hit CTCCAAAGCTATATACATCACACTTCTCATTTGAGGGTTCATTACGGAGA 6 0.15 No Hit CTCCCGTTCTGGTAATTATTGTCAGTCTTCCAGGTCTGTTGTGCGGGTTA 6 0.15 No Hit CTCTTCCACGTACTGATTGTTTGTGGTGCGCACCTGCTGGAGTTCAGAGT 6 0.15 No Hit GCTCCTAAAAGCAGCCTCACGAGCACCAGTGGTAAGGAGATACAAGGCTT 6 0.15 No Hit CGGTGGGTTTTAAAGTTAAGGTGGTCTTGGTGGTTGTGGTAGGGATTTGT 6 0.15 No Hit CAGCCATTTGGACATATGAAGGAGGTGGAACGCTCAAAGATGCGGCAAAA 6 0.15 No Hit CGTCAGGTGTGTTGCCGAGGACCAGCTTTTGCATGAACTGGACCATCTCC 6 0.15 No Hit CATCTGAGTATTCTGTGACAGTTTCACTCACTGTCATAGCACTGGCATCA 6 0.15 No Hit GCGCAGTGACAACAGTATCTCCACAATGATGCCTCGCTTCCCAGAAAAGC 5 0.125 No Hit GGCTGGGTGATTCCACAGAGCTTGTAGCTTCGATGTCGCCATGGATGAAG 5 0.125 No Hit GCTATATCTTCTTCACGTTTCCTAGCAGCTTCAGCTTTTCTAGCTGAGGA 5 0.125 No Hit CTTTGTAAAAAGTAAAGATCATTCCTCCACCCAAGAAGAGGAAGTCAACC 5 0.125 No Hit CCAACTCATGCTCATCACCTTCACCTTCACCTTCTCCAGACTCTTCCTCA 5 0.125 No Hit CGCCTTTCTTTTTGTCCACGGAGTTGGTTCCAGTATTCCTTTTCTGCCTC 5 0.125 No Hit CGGGTGTGAAGCAGTGTCAAACCAAAATACCCAACAAGCATTACCTCTCC 5 0.125 No Hit GCTTTCTTGTATTGTTCAAGACAGTTGGAAAAACGACGACTGGTGGCACA 5 0.125 No Hit TCTCACAAAAGCACGAGGGAACAACCGGGGGAAAATAGCATTTTTTGCTT 5 0.125 No Hit CCCCCTCCAAAAAAAAGGTAAAAATAGTAAAGTTGAAATTGCAAAAGAGA 5 0.125 No Hit CCGTACCAAATTGGACAATGCCGACTGAGAAGAATTTCAACAGAACCTAG 5 0.125 No Hit CCACAATGATGCCTCGCTTCCCAGAAAAGCGGAGATGCTCCTGCTCCTTT 5 0.125 No Hit GTTGTAATCTGCCAGGGTCCTTCCATCTTCAAGCTGCTTCCCAGCAAAGA 5 0.125 No Hit CGGATGTTCACAACAAATTAAGACAGGGAGATTAAGAACGAAAGAGACTT 5 0.125 No Hit ACTGAGGAGGCTCCACTATTCTCCTACTTCACTTCTTCCTCTTCTTTGGG 5 0.125 No Hit CCCGGTCTCATCGGAAGTCCCTTGGAGTTATGAAGTAGGGCTGGGGTAAC 5 0.125 No Hit CATTTTGACAAACCATCTTCCTTACATGCTCATTTTTTGTTAATTGCCGG 5 0.125 No Hit GTTCAAGAAGCCATCACCAACGGGGATCCATACCCATTTTGATGTGATTT 5 0.125 No Hit CCACTCTCGTAAAAGACCAAAAAAATATGAAGGTGGGCGGAGGGCTGACG 5 0.125 No Hit CTCCACTCCGTCGTCTTTCTCCTTCGGGCCGCCGTCAGCATCATCGGAAG 5 0.125 No Hit ACACTCGACCAAACAACATAACACTTGGGATGATTCTTGGGATCATCAGC 5 0.125 No Hit GTTCGTGGAAGATGCTGCCAACTGCCAACTGCCATCAATCAATTAGTACA 5 0.125 No Hit GTTAGTTGGCCTCACTCCTCCAAGCCTATTCAACTCCCCAGTTCGATCAC 5 0.125 No Hit ATTCGCTGCATTTCAAGGCACCTCAGAATCAATTATTTGGAGGAGTTATC 5 0.125 No Hit CTCCAGAGATCCCATTTTCATTCATCACAAGCATCAGTTCTAAATATCTG 5 0.125 No Hit GTGACTGTGATAGCCAAGAGTTACTGGAGGAGCCAGAGGAGGAGCAAGAG 5 0.125 No Hit GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGATACCAATCGCGGGT 5 0.125 TruSeq Adapter, Index 11 (97% over 39bp) CAAGCTGGCACCATAGGCGCTCTATTGTTGTGTATGGAAACCATGCCGCA 5 0.125 No Hit CTTGTGACCATAACTTGTGTGCCACTCTGTGTGCTTGGCATGCTAGAAAG 5 0.125 No Hit GTACATCAACACACATGGGCATGCACCTTGGATGCAAATTCCTATTACAT 5 0.125 No Hit GCCCATCCTTTGCATTCCTGTTGAAATTTCTTCCCAAAGTGGCCCCTTAG 5 0.125 No Hit ATGCACGATTTGGGTCATGGTTTCCTCGGAAAGAAGTGCACCGGTGGGGT 5 0.125 No Hit AGACGCATTGCCAATCATCAAACTAAAGATCATTTGCTTCTATGAGGGCC 5 0.125 No Hit CTCTTCAACTCCTCGTCACTGTCATTGCATTCATCTCCAGTGGGCTTTCT 5 0.125 No Hit AATGTCTCAATATAAAAACTACTTCAATGTCATTGTGTTTTCTGCAGTAC 5 0.125 No Hit CGAGCGAGGCTATCAATCTCTGCACTATTTTGAGAACCTTTACAGTCATG 5 0.125 No Hit CCTAGACAATGATGCAATTTAAGCGTTCAGACTTAAAGAGTTGAGTCCTA 5 0.125 No Hit GGCAGACGATTCATGTCCCGGTTGACGGCAATTGTAGCACAACCGCTGCT 5 0.125 No Hit TACCGTTTGGCCTTGTCCAAAGGCATCTTCCGGATTGAAAACGCAGTCTC 5 0.125 No Hit GGCAGCATTGTCTTGAATTTCAGCAAGCTATACAAAGAAATTCTCCCTCC 5 0.125 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0 0.0 0.0 0.0 0.0 64-65 0.0 0.0 0.0 0.0 0.0 66-67 0.0 0.0 0.0 0.0 0.0 68-69 0.0 0.0 0.0 0.0 0.0 70-71 0.0 0.0 0.0 0.0 0.0 72-73 0.0 0.0 0.0 0.0 0.0 74-75 0.025 0.0 0.0 0.0 0.0 76-77 0.025 0.0 0.0 0.0 0.0 78-79 0.0625 0.0 0.0 0.0 0.0 80-81 0.1 0.0 0.0 0.0 0.0 82-83 0.23750000000000002 0.0 0.0 0.0 0.0 84-85 0.375 0.0 0.0 0.0 0.0 86-87 0.4375 0.0 0.0 0.0 0.0 88-89 0.4875 0.0 0.0 0.0 0.0 90-91 0.525 0.0 0.0 0.0 0.0 92-93 0.7 0.0 0.0 0.0 0.0 94-95 0.9125000000000001 0.0 0.0 0.0 0.0 96-97 1.0125 0.0 0.0 0.0 0.0 98-99 1.1125 0.0 0.0 0.0 0.0 100-101 1.3 0.0 0.0 0.0 0.0 102-103 1.5875 0.0 0.0 0.0 0.0 104-105 1.8125 0.0 0.0 0.0 0.0 106-107 2.2375 0.0 0.0 0.0 0.0 108-109 2.4749999999999996 0.0 0.0 0.0 0.0 110-111 3.0125 0.0 0.0 0.0 0.0 112-113 3.3625 0.0 0.0 0.0 0.0 114-115 3.8125 0.0 0.0 0.0 0.0 116-117 4.275 0.0 0.0 0.0 0.0 118-119 4.775 0.0 0.0 0.0 0.0 120-121 5.5 0.0 0.0 0.0 0.0 122-123 5.9 0.0 0.0 0.0 0.0 124-125 6.375 0.0 0.0 0.0 0.0 126-127 6.7 0.0 0.0 0.0 0.0 128-129 7.1625 0.0 0.0 0.0 0.0 130-131 7.525 0.0 0.0 0.0 0.0 132-133 8.0 0.0 0.0 0.0 0.0 134-135 8.725 0.0 0.0 0.0 0.0 136-137 9.675 0.0 0.0 0.0 0.0 138-139 10.55 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content pass >>END_MODULE SRR26075383 read2 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR26075383_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.37975 37.0 37.0 37.0 37.0 37.0 2 36.383 37.0 37.0 37.0 37.0 37.0 3 36.2995 37.0 37.0 37.0 37.0 37.0 4 36.4315 37.0 37.0 37.0 37.0 37.0 5 36.4165 37.0 37.0 37.0 37.0 37.0 6 36.3325 37.0 37.0 37.0 37.0 37.0 7 36.426 37.0 37.0 37.0 37.0 37.0 8 36.3215 37.0 37.0 37.0 37.0 37.0 9 36.347 37.0 37.0 37.0 37.0 37.0 10-14 36.3206 37.0 37.0 37.0 37.0 37.0 15-19 36.3534 37.0 37.0 37.0 37.0 37.0 20-24 36.26879999999999 37.0 37.0 37.0 37.0 37.0 25-29 36.166000000000004 37.0 37.0 37.0 37.0 37.0 30-34 36.044599999999996 37.0 37.0 37.0 37.0 37.0 35-39 36.042 37.0 37.0 37.0 37.0 37.0 40-44 35.937799999999996 37.0 37.0 37.0 37.0 37.0 45-49 35.863 37.0 37.0 37.0 37.0 37.0 50-54 35.7685 37.0 37.0 37.0 37.0 37.0 55-59 35.774100000000004 37.0 37.0 37.0 37.0 37.0 60-64 35.849000000000004 37.0 37.0 37.0 37.0 37.0 65-69 35.7868 37.0 37.0 37.0 37.0 37.0 70-74 35.69690000000001 37.0 37.0 37.0 37.0 37.0 75-79 35.620999999999995 37.0 37.0 37.0 37.0 37.0 80-84 35.6845 37.0 37.0 37.0 37.0 37.0 85-89 35.6289 37.0 37.0 37.0 37.0 37.0 90-94 35.581 37.0 37.0 37.0 37.0 37.0 95-99 35.7515 37.0 37.0 37.0 37.0 37.0 100-104 35.5829 37.0 37.0 37.0 37.0 37.0 105-109 35.624300000000005 37.0 37.0 37.0 37.0 37.0 110-114 35.4258 37.0 37.0 37.0 37.0 37.0 115-119 35.446600000000004 37.0 37.0 37.0 37.0 37.0 120-124 35.35405 37.0 37.0 37.0 37.0 37.0 125-129 35.366099999999996 37.0 37.0 37.0 34.6 37.0 130-134 35.3769 37.0 37.0 37.0 34.6 37.0 135-139 35.1971 37.0 37.0 37.0 32.2 37.0 140-144 35.16969999999999 37.0 37.0 37.0 29.8 37.0 145-149 35.212599999999995 37.0 37.0 37.0 34.6 37.0 150-151 34.9185 37.0 37.0 37.0 25.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.0 1101 2 0.0 1101 3 0.0 1101 4 0.0 1101 5 0.0 1101 6 0.0 1101 7 0.0 1101 8 0.0 1101 9 0.0 1101 10-14 0.0 1101 15-19 0.0 1101 20-24 0.0 1101 25-29 0.0 1101 30-34 0.0 1101 35-39 0.0 1101 40-44 0.0 1101 45-49 0.0 1101 50-54 0.0 1101 55-59 0.0 1101 60-64 0.0 1101 65-69 0.0 1101 70-74 0.0 1101 75-79 0.0 1101 80-84 0.0 1101 85-89 0.0 1101 90-94 0.0 1101 95-99 0.0 1101 100-104 0.0 1101 105-109 0.0 1101 110-114 0.0 1101 115-119 0.0 1101 120-124 0.0 1101 125-129 0.0 1101 130-134 0.0 1101 135-139 0.0 1101 140-144 0.0 1101 145-149 0.0 1101 150-151 0.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 13 3.0 14 6.0 15 5.0 16 2.0 17 6.0 18 7.0 19 3.0 20 9.0 21 3.0 22 9.0 23 9.0 24 8.0 25 14.0 26 10.0 27 16.0 28 20.0 29 15.0 30 18.0 31 28.0 32 39.0 33 82.0 34 176.0 35 582.0 36 2691.0 37 239.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 39.034758689672415 21.980495123780948 13.4783695923981 25.506376594148538 2 29.375 26.700000000000003 28.1 15.825 3 23.275000000000002 29.349999999999998 28.525 18.85 4 26.075 33.050000000000004 20.724999999999998 20.150000000000002 5 25.5 36.7 20.525 17.275 6 22.85 36.925000000000004 22.225 18.0 7 22.575 22.525000000000002 34.925 19.975 8 23.549999999999997 24.45 27.150000000000002 24.85 9 22.900000000000002 25.85 27.950000000000003 23.3 10-14 25.124999999999996 29.26 24.349999999999998 21.265 15-19 25.040000000000003 28.33 26.25 20.380000000000003 20-24 24.05 28.68 25.990000000000002 21.279999999999998 25-29 25.509999999999998 28.804999999999996 25.924999999999997 19.759999999999998 30-34 24.365000000000002 27.785 26.715 21.135 35-39 25.495 28.655 25.430000000000003 20.419999999999998 40-44 25.745 28.655 25.635 19.965 45-49 24.66 27.605 26.555 21.18 50-54 24.39 28.335 27.189999999999998 20.085 55-59 24.37 28.365000000000002 27.27 19.994999999999997 60-64 25.069999999999997 27.57 26.655 20.705000000000002 65-69 24.545 28.73 26.290000000000003 20.435 70-74 25.53 28.46 25.590000000000003 20.419999999999998 75-79 24.65 28.349999999999998 25.759999999999998 21.240000000000002 80-84 25.2 28.38 25.495 20.925 85-89 25.71 28.065 25.83 20.395 90-94 24.745 27.35 27.189999999999998 20.715 95-99 25.040000000000003 28.515 26.55 19.895 100-104 26.115 28.185 26.229999999999997 19.470000000000002 105-109 26.185000000000002 27.655 26.365 19.794999999999998 110-114 24.895 28.27 26.035000000000004 20.8 115-119 25.695 28.015 26.840000000000003 19.45 120-124 25.191259562978146 28.30641532076604 26.45632281614081 20.046002300115006 125-129 25.929999999999996 28.38 25.945 19.744999999999997 130-134 27.150000000000002 28.915000000000003 25.230000000000004 18.705 135-139 26.662666266626662 27.582758275827583 26.542654265426542 19.21192119211921 140-144 26.825365073014602 28.900780156031207 25.0500100020004 19.22384476895379 145-149 27.485994397759107 28.441376550620245 25.97539015606242 18.097238895558224 150-151 27.731932983245812 27.894473618404604 26.11902975743936 18.254563640910227 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.5 7 0.5 8 0.5 9 1.5 10 1.5 11 1.5 12 1.0 13 0.0 14 0.0 15 0.0 16 0.5 17 0.5 18 2.0 19 2.5 20 1.0 21 0.5 22 1.5 23 2.5 24 1.5 25 1.0 26 4.0 27 4.5 28 2.0 29 3.0 30 4.5 31 12.0 32 15.5 33 12.5 34 21.5 35 34.5 36 48.5 37 69.0 38 103.5 39 146.5 40 159.5 41 194.0 42 261.5 43 277.5 44 293.5 45 295.5 46 280.5 47 267.5 48 244.0 49 236.5 50 189.5 51 168.0 52 153.5 53 98.0 54 61.5 55 60.0 56 59.0 57 37.5 58 25.5 59 21.0 60 20.0 61 14.5 62 8.5 63 5.5 64 4.0 65 5.5 66 5.0 67 3.5 68 3.0 69 1.5 70 2.0 71 2.5 72 1.0 73 0.0 74 2.0 75 2.0 76 0.0 77 0.0 78 0.5 79 0.5 80 0.5 81 0.5 82 2.0 83 2.5 84 1.0 85 2.5 86 3.0 87 3.0 88 2.5 89 3.0 90 2.5 91 0.5 92 0.5 93 1.0 94 1.0 95 0.0 96 0.5 97 0.5 98 0.5 99 1.0 100 2.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.025 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.005 125-129 0.0 130-134 0.0 135-139 0.01 140-144 0.02 145-149 0.04 150-151 0.025 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 62.8 #Duplication Level Percentage of deduplicated Percentage of total 1 67.11783439490446 42.15 2 18.471337579617835 23.200000000000003 3 8.240445859872612 15.525 4 3.2643312101910826 8.200000000000001 5 1.6321656050955413 5.125 6 0.5573248407643312 2.1 7 0.3184713375796179 1.4000000000000001 8 0.15923566878980894 0.8 9 0.11942675159235669 0.675 >10 0.11942675159235669 0.8250000000000001 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGT 13 0.325 No Hit GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG 10 0.25 No Hit CGGGAATCCAGTTAGGCCTCTGAATGCACTTGGATCAGCCATTGCAATAT 10 0.25 No Hit CTTGAATCCTGGTCAAGGTGGGCAGCTTATGGTATTTTGCATGATCCTGA 9 0.22499999999999998 No Hit GGAGGATTTGGCACTGTTTATTATGGTGTACTCAGGGATGGGCATGAGGT 9 0.22499999999999998 No Hit GGACGAATTCGCTCATTTTCTTCAATTTCTCTAAAACTAAGAGTGCTCTG 9 0.22499999999999998 No Hit CTGCCATGGCGCATTAGAGCCTGCAAATGATACGAGAAAGGAAAAACCCT 8 0.2 No Hit TGAAGCTGTCAAGAGCATTGAGATCAAGGTTCCAGAGAAACCCAAAGCAC 8 0.2 No Hit ATAGTGGAGAAGTATGTGCTGTTACTAATCCACTGTTGGCTGCACCACCA 8 0.2 No Hit TGGAACTACAACTACGGGCAGTGTGGAAAAGCTATAGGAGTGGATCTATT 8 0.2 No Hit GCTTTGTATGAGGCTTTTGGATTGACTGTTGTTGAGGCCATGACATGTGG 7 0.17500000000000002 No Hit AGCTGCAGCATATCTTGAACTGGGAAGTTTCCATCAAGCTGAAGCAGATT 7 0.17500000000000002 No Hit ATACAGGAAGAAGAGATTGACAGAAATGAGGGAGGCTACAAAGATTGTGA 7 0.17500000000000002 No Hit GGCGCATACAGTTACATTGCACCACGCCTATCCACTGCCATGAAAGCACT 7 0.17500000000000002 No Hit TTGGAAGATATCTTTATTGGAGAAACAGTAGTGCCATATTGTACTCTGAT 7 0.17500000000000002 No Hit GATGATGTGCATAAGTGCATTAAATACAATGTCTGGGCCAGCACACCAAA 7 0.17500000000000002 No Hit AACTTTATGACCCATCCGAGTTCCATGTGGTTAACCCGATGAAAAGGACT 7 0.17500000000000002 No Hit CGACCACCCACAAAAACAAGTAAATCATGGCTCGTGTCGCTGGGTTGGTG 7 0.17500000000000002 No Hit GGATTTCTCAGGTGCTGCTTTGGATGAATTCAAAAGAGAAGTTCGAATAA 6 0.15 No Hit CGGCAGTTGGATGGAGCACATGTTGAATTCCTACGTGGTGTATCCAATCC 6 0.15 No Hit TGACAACATAGACCACTTATTTGATCTGCCTGATGGGCGTTTTTATGCGG 6 0.15 No Hit GGAAGGCCCAATGCCCAATTGTTGAAAGGCTTACAAACTCACTGCAGATG 6 0.15 No Hit GGAGAGGATATTGCTTTGTTAAATGCATTGAAAGTATTTTCTAAGGATGT 6 0.15 No Hit CGGAAACAAATCAAGAGTATGCCAGGAGCCATTTTCCCATGATGGACAAT 6 0.15 No Hit GAGAAACACACGCCCCCTCCTCTGGTTTCCTTTGTCTTAGTCTACAAAGA 6 0.15 No Hit AAATGGTTACCACCACCATGTCCCAGACAACCTCACTAGAGACCAATATG 6 0.15 No Hit GTCTGAGTCAACTTATTGATCCTTCAATGCAAGGGAATTATCCTTCAGGA 6 0.15 No Hit ACGCCTGTTTTAGCTCTTCCTGCTCCAGATGGAACAGTCCAGGCAGTTAA 6 0.15 No Hit CAAGGAGCGGTTGCTCCACTTTACCAAATATTTCAATCTTCCATAAACTC 6 0.15 No Hit TGAAAGTTGAGGAGGATCAGCCACTTTCGGTCTCATCAAAGAGGTACAAG 6 0.15 No Hit ATGTGCTCACCGACGACTCATCCAGGCTCCTTCCGTTGTAGTCTCCATAA 6 0.15 No Hit GGGAGTACACCTCAGGTTGATCTTGCCCATCTGTTAGCAGCTCGTGAACA 6 0.15 No Hit CTTCCAATTTCACAGGCTCCCATGATAGCCAAGGAAGCACCAAGAGCTTA 5 0.125 No Hit TGATGACTATACAGATTGGAATAAGTGGATAAATAACCGTGGAGGGATTG 5 0.125 No Hit ACAGAAGCTGCAGCGATGGTGAAGTATTCAAGAGAACCAGATAACCAAAC 5 0.125 No Hit GCATGCCCGTGGTTGGCGGTCAATTTACTGCATGCCCAAGCGCCCAGCCT 5 0.125 No Hit AGAGATTCGCGCTTTCAGAGACGACGATGGGATCCGGAGAGGAGAGAACA 5 0.125 No Hit GATATTCTTTCCTAATTGGGGAAAGGTTAAAAACTTAATCCAAGATTAGG 5 0.125 No Hit AAAAACCATCACCCTGGAGGTTGAGAGTTCAGACACCATTGATAATGTGA 5 0.125 No Hit ATACCACGAAACGATTGCGGAGACGCCAGCAAGATAGAGGAGGGCAAGCC 5 0.125 No Hit TTTCCATGTAGAATTTCTTCCATTGTTACAAATGTGGCTGCTGCTACTCG 5 0.125 No Hit GGACAGGACCTGGGGGATGGCTGGCCAAGTATCAAATCCTGCATCTTTGT 5 0.125 No Hit GCTAGGAAGTCCTTGAACAGGCCATCTTCCCGGAAGGTACTCGCCAATTT 5 0.125 No Hit TAGGAATCTACAATGTTGGCACTGGAAAGGGTAGATCAGTCAAGGAGTTT 5 0.125 No Hit CAACAATGCGGGTAACTCCATTTTGGCATTAACATCAAATGCCTCCCATT 5 0.125 No Hit GGGAAGGAAAAACCTAACCCAGCTGCTTCCAAGGATGACAAGAATGTTGT 5 0.125 No Hit GCAAGCGGGCAAGCAATCAGTGTATCAGAGAAAGCTAAAGGAAGAAAGGA 5 0.125 No Hit CAAACCAATTCCCTCAGCTCAAGTCCTAAGCAGTCTCGAGCAACTCCTCT 5 0.125 No Hit ATTTATTCCTTAACAGGGAAGAAACAGACACAGAGAACCGAATAGATCTC 5 0.125 No Hit CTATAATGTGTTTGATTTTCTGAGAGAAATTGTCAGCAGGGTTCCTGACT 5 0.125 No Hit CAAATCCCAAGAAGCCATTTGCTGCAATTGTTGGTGGCTCAAAGGTATCA 5 0.125 No Hit ATTGATCCAACAAAGGTGGTCAGATGTTGCCTGGAGCATGCATCATCGGT 5 0.125 No Hit GGAGAAGACTGGGGCGACAACGGTTACTTTAAGATGGAGATGGGGAAGAA 5 0.125 No Hit GTCATTTTCCAGTGGATTATTCACGCATGTGGTTTTTCGTGGTGGCCATA 5 0.125 No Hit CAAACCTCATCTTCGTTACACGCGGAGGAGGCAGTGTGTTGTTGGCCAGT 5 0.125 No Hit GTGGAGGAGGATTTGAGAGCTGTAAAAGAGAGAGACCCTGCATGCATGAG 5 0.125 No Hit TTCTTGATGACTATACAGATTGGAATAAGTGGATAAATAACCGTGGAGGG 5 0.125 No Hit CTCGATGAGAGCCAGGACTGGGCCACCTCCATAGCTGAGCTCGAGCAGGC 5 0.125 No Hit GCCCGAGCTAAACCCAAAATTCAAGCCTGAATCTATTGTTGAATATCTCA 5 0.125 No Hit AAGGCATTGATGAGGTGAAGGAAATGTTTGAGAAATTCCCAGATAGGCTT 5 0.125 No Hit TGAATTCGTTCCCGGGCCTTGTACACACCGCCCGTCACACTATGGGAGCT 5 0.125 No Hit ATTTTAGTTGATTCTCCTTCTGCTCACGAGTCTGGTGGCGAGGTCCTTGA 5 0.125 No Hit GCCAGCGTCATCAAGATGGCCAAAGCCAGAAGTTCTTGCACTTATAAAGT 5 0.125 No Hit CATAACAAAGCTCCAAAACTCGGTGGAGGATATCATGGCCACCATGGCGA 5 0.125 No Hit GGTGCTACCTGCTCTATTTGTCTAGAGCATCCTCACAACGCAGTGCTTCT 5 0.125 No Hit AAAACACCAGTATTTCTGCCTTGCCAGAGAGACCTTCTGAGCCCCCATGT 5 0.125 No Hit TTCAATGAAGAACTCCTGATTTTGTATGGAGTTGGATGATTATACACACT 5 0.125 No Hit AGAAGAAGTTGAGGAGGTAGAGAATGAGGACGAGGAAGAAGAAGATGAAG 5 0.125 No Hit ATTGGAAGCAAGGAAGGAGAAGAAAAAAACTTCAAAGAAAATGGGCGGCG 5 0.125 No Hit CATCATCAAGAAAGAACCAAGAAGTGGTTCTTGCTTTCTATGAAGCCTTA 5 0.125 No Hit GGGCTACAAGAGGAGGTCTTATCAAAACCGATTGGAGCCAAGCACCGTTT 5 0.125 No Hit TGGAGGTTTTGGGTGGGATGGAGTTCTATGTTTATCATTGTATCCGCATG 5 0.125 No Hit GTGGATTTGTATAGGAAGCTTGTTGTCGGGTCTGCTTGGAGGAAACAAAT 5 0.125 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0 0.0 0.0 0.0 0.0 64-65 0.0 0.0 0.0 0.0 0.0 66-67 0.0 0.0 0.0 0.0 0.0 68-69 0.0 0.0 0.0 0.0 0.0 70-71 0.0 0.0 0.0 0.0 0.0 72-73 0.0 0.0 0.0 0.0 0.0 74-75 0.025 0.0 0.0 0.0 0.0 76-77 0.025 0.0 0.0 0.0 0.0 78-79 0.0625 0.0 0.0 0.0 0.0 80-81 0.1 0.0 0.0 0.0 0.0 82-83 0.23750000000000002 0.0 0.0 0.0 0.0 84-85 0.375 0.0 0.0 0.0 0.0 86-87 0.4375 0.0 0.0 0.0 0.0 88-89 0.4875 0.0 0.0 0.0 0.0 90-91 0.525 0.0 0.0 0.0 0.0 92-93 0.7 0.0 0.0 0.0 0.0 94-95 0.9125000000000001 0.0 0.0 0.0 0.0 96-97 1.0125 0.0 0.0 0.0 0.0 98-99 1.1125 0.0 0.0 0.0 0.0 100-101 1.3 0.0 0.0 0.0 0.0 102-103 1.6124999999999998 0.0 0.0 0.0 0.0 104-105 1.8624999999999998 0.0 0.0 0.0 0.0 106-107 2.3 0.0 0.0 0.0 0.0 108-109 2.55 0.0 0.0 0.0 0.0 110-111 3.0875 0.0 0.0 0.0 0.0 112-113 3.4375 0.0 0.0 0.0 0.0 114-115 3.8875 0.0 0.0 0.0 0.0 116-117 4.3375 0.0 0.0 0.0 0.0 118-119 4.85 0.0 0.0 0.0 0.0 120-121 5.575 0.0 0.0 0.0 0.0 122-123 5.9875 0.0 0.0 0.0 0.0 124-125 6.475 0.0 0.0 0.0 0.0 126-127 6.825 0.0 0.0 0.0 0.0 128-129 7.3 0.0 0.0 0.0 0.0 130-131 7.675 0.0 0.0 0.0 0.0 132-133 8.15 0.0 0.0 0.0 0.0 134-135 8.875 0.0 0.0 0.0 0.0 136-137 9.825 0.0 0.0 0.0 0.0 138-139 10.725000000000001 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ACCAAAT 10 0.006830828 145.0 6 CAAATGA 10 0.006830828 145.0 8 >>END_MODULE Read 1131085 spots for SRR26075383.sra Written 1131085 spots for SRR26075383.sra Read 1131085 spots for SRR26075383.sra Written 1131085 spots for SRR26075383.sra Read 1131085 spots for SRR26075383.sra Written 1131085 spots for SRR26075383.sra Read 1131085 spots for SRR26075383.sra Written 1131085 spots for SRR26075383.sra Read 1131085 spots for SRR26075383.sra Written 1131085 spots for SRR26075383.sra Read 1131085 spots for SRR26075383.sra Written 1131085 spots for SRR26075383.sra Read 1131085 spots for SRR26075383.sra Written 1131085 spots for SRR26075383.sra Read 1131085 spots for SRR26075383.sra Written 1131085 spots for SRR26075383.sra Read 1131085 spots for SRR26075383.sra Written 1131085 spots for SRR26075383.sra Read 1131085 spots for SRR26075383.sra Written 1131085 spots for SRR26075383.sra Read 1131085 spots for SRR26075383.sra Written 1131085 spots for SRR26075383.sra Read 1131085 spots for SRR26075383.sra Written 1131085 spots for SRR26075383.sra Read 1131085 spots for SRR26075383.sra Written 1131085 spots for SRR26075383.sra Read 1131085 spots for SRR26075383.sra Written 1131085 spots for SRR26075383.sra Read 1131085 spots for SRR26075383.sra Written 1131085 spots for SRR26075383.sra Read 1131102 spots for SRR26075383.sra Written 1131102 spots for SRR26075383.sra Read 1131085 spots for SRR26075383.sra Written 1131085 spots for SRR26075383.sra Read 1131085 spots for SRR26075383.sra Written 1131085 spots for SRR26075383.sra Read 1131085 spots for SRR26075383.sra Written 1131085 spots for SRR26075383.sra Read 1131085 spots for SRR26075383.sra Written 1131085 spots for SRR26075383.sra SRR ids: ['SRR26075383.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_8_ad2wmx SRR26075383.sra spots: 22621717 blocks: [[1, 1131085], [1131086, 2262170], [2262171, 3393255], [3393256, 4524340], [4524341, 5655425], [5655426, 6786510], [6786511, 7917595], [7917596, 9048680], [9048681, 10179765], [10179766, 11310850], [11310851, 12441935], [12441936, 13573020], [13573021, 14704105], [14704106, 15835190], [15835191, 16966275], [16966276, 18097360], [18097361, 19228445], [19228446, 20359530], [20359531, 21490615], [21490616, 22621717]] SRR26075383 file size 8350014 SRR26075383 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o SRR26075383 SRR26075383_1.fastq SRR26075383_2.fastq Input file: SRR26075383_1.fastq Paired file: SRR26075383_2.fastq trimmed: SRR26075383-trimmed-pair1.fastq, SRR26075383-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Wed Feb 12 03:39:26 2025 >> started Wed Feb 12 03:39:51 2025 >> done (24.563s) 22621717 read pairs processed; of these: 107 ( 0.00%) short read pairs filtered out after trimming by size control 153109 ( 0.68%) empty read pairs filtered out after trimming by size control 22468501 (99.32%) read pairs available; of these: 3331874 (14.83%) trimmed read pairs available after processing 19136627 (85.17%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 18 11 0.00% 19 10 0.00% 20 21 0.00% 21 17 0.00% 22 18 0.00% 23 8 0.00% 24 16 0.00% 25 9 0.00% 26 15 0.00% 27 18 0.00% 28 20 0.00% 29 21 0.00% 30 25 0.00% 31 19 0.00% 32 26 0.00% 33 21 0.00% 34 35 0.00% 35 34 0.00% 36 18 0.00% 37 20 0.00% 38 37 0.00% 39 27 0.00% 40 43 0.00% 41 53 0.00% 42 56 0.00% 43 30 0.00% 44 54 0.00% 45 43 0.00% 46 68 0.00% 47 63 0.00% 48 64 0.00% 49 76 0.00% 50 89 0.00% 51 105 0.00% 52 146 0.00% 53 136 0.00% 54 156 0.00% 55 183 0.00% 56 166 0.00% 57 207 0.00% 58 204 0.00% 59 267 0.00% 60 321 0.00% 61 369 0.00% 62 469 0.00% 63 439 0.00% 64 575 0.00% 65 684 0.00% 66 657 0.00% 67 779 0.00% 68 934 0.00% 69 1069 0.00% 70 1234 0.01% 71 1404 0.01% 72 1732 0.01% 73 1945 0.01% 74 2176 0.01% 75 2373 0.01% 76 2760 0.01% 77 3082 0.01% 78 3370 0.01% 79 4013 0.02% 80 4449 0.02% 81 5103 0.02% 82 5454 0.02% 83 6339 0.03% 84 7455 0.03% 85 8178 0.04% 86 8958 0.04% 87 9555 0.04% 88 10508 0.05% 89 11123 0.05% 90 11499 0.05% 91 12796 0.06% 92 14299 0.06% 93 15239 0.07% 94 16792 0.07% 95 17942 0.08% 96 19867 0.09% 97 20739 0.09% 98 21964 0.10% 99 22489 0.10% 100 24190 0.11% 101 25299 0.11% 102 26614 0.12% 103 28249 0.13% 104 29858 0.13% 105 31555 0.14% 106 33142 0.15% 107 34587 0.15% 108 35539 0.16% 109 37100 0.17% 110 37621 0.17% 111 39604 0.18% 112 40616 0.18% 113 41545 0.18% 114 44078 0.20% 115 45325 0.20% 116 47162 0.21% 117 48648 0.22% 118 49780 0.22% 119 51608 0.23% 120 52417 0.23% 121 53605 0.24% 122 54285 0.24% 123 56271 0.25% 124 58557 0.26% 125 59339 0.26% 126 61794 0.28% 127 63256 0.28% 128 65350 0.29% 129 65754 0.29% 130 67663 0.30% 131 67979 0.30% 132 69994 0.31% 133 71836 0.32% 134 72036 0.32% 135 74095 0.33% 136 77424 0.34% 137 76776 0.34% 138 78551 0.35% 139 80411 0.36% 140 81279 0.36% 141 83140 0.37% 142 83903 0.37% 143 84956 0.38% 144 87714 0.39% 145 88530 0.39% 146 88921 0.40% 147 90693 0.40% 148 92106 0.41% 149 93216 0.41% 150 94115 0.42% 151 19136627 85.17% 22468501 reads passed initial QC criterion=sequence-density sequence-density=0.18 sequence-density-rank=1 fanout-score=14.59 fanout-score-rank=7 prefix-density=0.32 prefix-fanout=8.0 sequence=CATCCTTCACAA criterion=fanout-score sequence-density=0.11 sequence-density-rank=19 fanout-score=53.01 fanout-score-rank=1 prefix-density=0.37 prefix-fanout=15.1 sequence=ATCTCCTTCTTGC criterion=sequence-density sequence-density=0.36 sequence-density-rank=1 fanout-score=2.77 fanout-score-rank=31 prefix-density=0.36 prefix-fanout=2.8 sequence=ATGTACCCTGAC criterion=fanout-score sequence-density=0.01 sequence-density-rank=39 fanout-score=204.23 fanout-score-rank=1 prefix-density=0.13 prefix-fanout=13.3 sequence=AGAGAAAAGATAAGCTAGGCAAGATGGTTTTACTAGACAAGATGTGGGATGATGTTGTTGCTGGACCTCAGCCAGAACGTGGCCTTGG SRR26075383 testing PE reads STAR mapping to Ensembl genome Started job on | Feb 12 03:40:36 Started mapping on | Feb 12 03:40:36 Finished on | Feb 12 03:44:20 Mapping speed, Million of reads per hour | 361.10 Number of input reads | 22468501 Average input read length | 294 UNIQUE READS: Uniquely mapped reads number | 20109443 Uniquely mapped reads % | 89.50% Average mapped length | 293.31 Number of splices: Total | 18685388 Number of splices: Annotated (sjdb) | 18224240 Number of splices: GT/AG | 18327035 Number of splices: GC/AG | 276349 Number of splices: AT/AC | 25526 Number of splices: Non-canonical | 56478 Mismatch rate per base, % | 0.40% Deletion rate per base | 0.03% Deletion average length | 3.12 Insertion rate per base | 0.02% Insertion average length | 2.47 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 641517 % of reads mapped to multiple loci | 2.86% Number of reads mapped to too many loci | 122255 % of reads mapped to too many loci | 0.54% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 6.75% % of reads unmapped: other | 0.35% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 1717541 1717541 1717541 N_multimapping 641517 641517 641517 N_noFeature 494555 19920440 588777 N_ambiguous 217733 1331 122181 UnstrandedReadsAssigned:19397155 PositiveStrandReadsAssigned:187672 NegativeStrandReadsAssigned:19398485 Dataset is classified negative stranded MeadianReadLen=151 20thPercentileLength=151 echo kmer=147 SRR26075383 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,400 [index] number of k-mers: 62,057,036 [index] number of equivalence classes: 130,681 [quant] running in paired-end mode [quant] will process pair 1: SRR26075383-trimmed-pair1.fastq SRR26075383-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 22,468,501 reads, 19,709,425 reads pseudoaligned [quant] estimated average fragment length: 216.41 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,149 rounds 52401 SRR26075383.ke.tsv 34699 SRR26075383.se.tsv 87100 total ==> SRR26075383.ke.tsv <== target_id length eff_length est_counts tpm Potri.005G200100.1.v4.1 2018 1802.59 2867 67.908 Potri.005G024800.1.v4.1 1035 819.59 2499 130.185 Potri.004G059700.1.v4.1 961 745.596 15 0.85897 Potri.007G009000.2.v4.1 1416 1200.59 0 0 Potri.003G141000.2.v4.1 2943 2727.59 1117 17.485 Potri.016G087400.1.v4.1 270 88.3611 2298 1110.4 Potri.015G069301.1.v4.1 564 350.283 0 0 Potri.010G195200.1.v4.1 1773 1557.59 585 16.0359 Potri.012G127500.1.v4.1 977 761.596 3448 193.301 ==> SRR26075383.se.tsv <== Potri.001G166300.v4.1 0 Potri.001G448400.v4.1 41 Potri.001G233950.v4.1 0 Potri.001G122700.v4.1 279 Potri.001G212900.v4.1 0 Potri.001G182400.v4.1 0 Potri.001G256600.v4.1 0 Potri.001G040500.v4.1 11 Potri.001G416900.v4.1 0 Potri.001G452600.v4.1 592 SRR26075383 completed mapping pipeline successfully