Starting /dee2/code/volunteer_pipeline.sh SRR26075384 current disk space = 3049004150784 free memory = 1500190780 SRR26075384 SRAfilesize 240c6bd6319ee55b62794add2b841dd1 SRR26075384.sra SRR26075384.sra file validated SRR26075384 is paired end SRR26075384 is conventional basespace SRR26075384 read1 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR26075384_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.625 37.0 37.0 37.0 37.0 37.0 2 36.649 37.0 37.0 37.0 37.0 37.0 3 36.632 37.0 37.0 37.0 37.0 37.0 4 36.7215 37.0 37.0 37.0 37.0 37.0 5 36.7345 37.0 37.0 37.0 37.0 37.0 6 36.6955 37.0 37.0 37.0 37.0 37.0 7 36.657 37.0 37.0 37.0 37.0 37.0 8 36.6305 37.0 37.0 37.0 37.0 37.0 9 36.5645 37.0 37.0 37.0 37.0 37.0 10-14 36.67265 37.0 37.0 37.0 37.0 37.0 15-19 36.6152 37.0 37.0 37.0 37.0 37.0 20-24 36.560900000000004 37.0 37.0 37.0 37.0 37.0 25-29 36.46310000000001 37.0 37.0 37.0 37.0 37.0 30-34 36.39919999999999 37.0 37.0 37.0 37.0 37.0 35-39 36.293 37.0 37.0 37.0 37.0 37.0 40-44 36.2354 37.0 37.0 37.0 37.0 37.0 45-49 36.2379 37.0 37.0 37.0 37.0 37.0 50-54 36.142700000000005 37.0 37.0 37.0 37.0 37.0 55-59 36.172900000000006 37.0 37.0 37.0 37.0 37.0 60-64 36.0972 37.0 37.0 37.0 37.0 37.0 65-69 36.052 37.0 37.0 37.0 37.0 37.0 70-74 36.0744 37.0 37.0 37.0 37.0 37.0 75-79 35.979 37.0 37.0 37.0 37.0 37.0 80-84 35.971000000000004 37.0 37.0 37.0 37.0 37.0 85-89 35.9366 37.0 37.0 37.0 37.0 37.0 90-94 35.812799999999996 37.0 37.0 37.0 37.0 37.0 95-99 35.7813 37.0 37.0 37.0 37.0 37.0 100-104 35.763600000000004 37.0 37.0 37.0 37.0 37.0 105-109 35.632600000000004 37.0 37.0 37.0 37.0 37.0 110-114 35.459399999999995 37.0 37.0 37.0 37.0 37.0 115-119 35.460300000000004 37.0 37.0 37.0 37.0 37.0 120-124 35.422399999999996 37.0 37.0 37.0 37.0 37.0 125-129 35.2843 37.0 37.0 37.0 34.6 37.0 130-134 35.28 37.0 37.0 37.0 34.6 37.0 135-139 35.1233 37.0 37.0 37.0 29.8 37.0 140-144 35.0739 37.0 37.0 37.0 25.0 37.0 145-149 35.11985 37.0 37.0 37.0 27.4 37.0 150-151 34.98224999999999 37.0 37.0 37.0 25.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.0 1101 2 0.0 1101 3 0.0 1101 4 0.0 1101 5 0.0 1101 6 0.0 1101 7 0.0 1101 8 0.0 1101 9 0.0 1101 10-14 0.0 1101 15-19 0.0 1101 20-24 0.0 1101 25-29 0.0 1101 30-34 0.0 1101 35-39 0.0 1101 40-44 0.0 1101 45-49 0.0 1101 50-54 0.0 1101 55-59 0.0 1101 60-64 0.0 1101 65-69 0.0 1101 70-74 0.0 1101 75-79 0.0 1101 80-84 0.0 1101 85-89 0.0 1101 90-94 0.0 1101 95-99 0.0 1101 100-104 0.0 1101 105-109 0.0 1101 110-114 0.0 1101 115-119 0.0 1101 120-124 0.0 1101 125-129 0.0 1101 130-134 0.0 1101 135-139 0.0 1101 140-144 0.0 1101 145-149 0.0 1101 150-151 0.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 21 2.0 22 6.0 23 3.0 24 9.0 25 6.0 26 10.0 27 21.0 28 28.0 29 39.0 30 42.0 31 31.0 32 55.0 33 84.0 34 149.0 35 439.0 36 2884.0 37 192.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 37.56256256256256 14.114114114114114 9.45945945945946 38.86386386386386 2 18.75 15.174999999999999 32.65 33.425 3 15.85 19.0 29.15 36.0 4 22.025 21.4 25.825 30.75 5 25.7 28.575 24.95 20.775 6 22.75 31.65 23.400000000000002 22.2 7 17.125 28.925 35.725 18.224999999999998 8 19.025 27.800000000000004 31.6 21.575 9 17.025000000000002 23.799999999999997 35.175 24.0 10-14 19.51097554877744 29.476473823691187 28.741437071853593 22.271113555677786 15-19 20.055 27.96 27.625 24.36 20-24 20.995 26.965 27.939999999999998 24.099999999999998 25-29 20.674999999999997 28.375 27.125 23.825 30-34 20.375 28.435 27.865000000000002 23.325000000000003 35-39 20.595 27.115000000000002 28.225 24.065 40-44 21.175 26.91 27.900000000000002 24.015 45-49 19.975 27.229999999999997 28.860000000000003 23.935000000000002 50-54 20.385 28.395 27.58 23.64 55-59 20.200000000000003 27.800000000000004 27.860000000000003 24.14 60-64 20.71 27.450000000000003 27.810000000000002 24.03 65-69 20.28 27.79 27.375 24.555 70-74 20.075000000000003 28.28 27.455000000000002 24.19 75-79 20.830000000000002 27.61 28.375 23.185 80-84 20.175 27.825 28.42 23.580000000000002 85-89 19.77 26.765 28.599999999999998 24.865000000000002 90-94 20.94 26.419999999999998 28.42 24.22 95-99 21.02 27.205000000000002 28.265 23.51 100-104 20.365 27.27 28.355000000000004 24.01 105-109 20.87 28.449999999999996 26.6 24.08 110-114 21.705 27.255000000000003 27.634999999999998 23.405 115-119 21.19 28.03 27.605 23.175 120-124 21.529999999999998 27.685 27.089999999999996 23.695 125-129 21.04 27.26 27.68 24.02 130-134 21.22 27.61 27.005000000000003 24.165 135-139 21.445 27.765 26.979999999999997 23.810000000000002 140-144 21.4 26.68 26.939999999999998 24.98 145-149 21.911095554777738 27.296364818240914 27.001350067503378 23.791189559477974 150-151 21.837500000000002 27.025 26.6625 24.474999999999998 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.5 10 0.5 11 0.0 12 0.0 13 0.0 14 0.0 15 0.5 16 0.5 17 1.0 18 2.0 19 1.5 20 1.0 21 1.0 22 1.5 23 1.5 24 1.5 25 1.5 26 3.5 27 6.0 28 10.5 29 16.5 30 13.5 31 17.5 32 28.0 33 32.5 34 41.5 35 57.5 36 82.0 37 94.0 38 111.0 39 144.5 40 170.0 41 215.0 42 244.5 43 254.0 44 267.5 45 267.5 46 272.0 47 256.0 48 215.5 49 190.5 50 187.5 51 156.5 52 134.0 53 120.0 54 87.0 55 72.0 56 54.0 57 31.0 58 19.0 59 21.5 60 22.5 61 15.5 62 13.5 63 10.5 64 3.5 65 4.0 66 5.0 67 3.0 68 5.5 69 5.5 70 0.5 71 0.5 72 1.0 73 0.5 74 0.0 75 0.0 76 0.0 77 0.0 78 0.5 79 0.5 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.1 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.005 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.005 150-151 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 57.175 #Duplication Level Percentage of deduplicated Percentage of total 1 59.160472234368164 33.825 2 22.73721031919545 26.0 3 9.881941407958024 16.950000000000003 4 4.285089637079143 9.8 5 1.7927415828596416 5.125 6 1.3117621337997376 4.5 7 0.3935286401399213 1.575 8 0.13117621337997376 0.6 9 0.21862702229995626 1.125 >10 0.08745080891998251 0.5 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GGGCGAGTCCTGCTGAGAACCGCTACTATCGGATAGTAAAGATGATAAAG 10 0.25 No Hit CTTCATCAAGTATAATCGGCTGAAGCTCTGTCCAGAAACCCCGTTGATTT 10 0.25 No Hit GCACCATGCTTGACAGCTTGTGGAGGTCGTTTCTGAACAGGGGGTGGTTT 9 0.22499999999999998 No Hit ATCCATTATATCTAAGCATGCTTTTCTCACCTTATCGGGGACTAATACAG 9 0.22499999999999998 No Hit CTTCTCTTCGTTTGTTGCAGCCATAACGTGTGAAACAATTTCCGAAGTTG 9 0.22499999999999998 No Hit GCCTCATCACTGTTAGGATCAGCCTTATAAGCAGAACCCTCTATGTACCC 9 0.22499999999999998 No Hit AATGAAAACATCCTTGGCAAATGCTTTCGCAGTTGTTCGTCTTTCATAAA 9 0.22499999999999998 No Hit GGACATTATGATAATCATGTAACTCACGGAGGAGGTTTTGAGCAATCACC 8 0.2 No Hit CTTTCATCCTTGCCTGAATTACTAGAACTGCCCCCGCTGGTTGCTACGTG 8 0.2 No Hit CCTCGGTTGTATAGATTGCTGGAACCAGACATCTGATACTTCAAATCCTC 8 0.2 No Hit GATTACAATCACATTCTTTATCCATTCATTTTCGCATTCATGAAATCACA 7 0.17500000000000002 No Hit CTGAGAAACAACAAAGTCTACTGAATCTGCAATAAATGGTTCATGAGGAC 7 0.17500000000000002 No Hit GTCTCTTCTGCAGGCCTATCCAACTTCACACCAACATCTTCAGAGTAGTC 7 0.17500000000000002 No Hit CTCCACAAAATGATCTGTTATGTTATAAATGTAAGATTAACTGCGTTATG 7 0.17500000000000002 No Hit CTGGATCATCCACACACATATGCCAATCTTTGTTCTTCCAATCAATCTCA 7 0.17500000000000002 No Hit GATCATCTTTGTAGTCGAAAGAGGACAAATCAAAGGGGGAGCCGAACATC 7 0.17500000000000002 No Hit GAAAAAAGATCACACGAATCGGTTAAATGGCTTTCGCGTGGCAGGCATCG 7 0.17500000000000002 No Hit GTTGTTGGTGCTGGAAGTGGCGGTGGCGGTGGCCTCTCCTCTTCCTATTT 7 0.17500000000000002 No Hit GCAGAAGTGAGAGATTGTAATTTCATCGCCAAGGCCATGTTTCCTTCCAG 7 0.17500000000000002 No Hit CCCAGCTTCTACTCGGATCCATGACAGAAATAATCCCAGTAAAGTTAGGA 6 0.15 No Hit GTTGGAAGTTTGCACTGAGCTGTCTTCTTCCTACAGCTACAGCCTTCATT 6 0.15 No Hit AAGCAACAAAAATAACACACAATAGATTATCAAATTATCAAGTCAAAAGA 6 0.15 No Hit GCCCGCAGTCTCCCAGTCACCTGCATGTATATCAACTCCTTGGATATGCT 6 0.15 No Hit GCGGGATTGAGCTTCGTTCACGGTGATGTTACGGCCATCGAGGTCCTGAC 6 0.15 No Hit CCGCAAATGTCTCGAGTGGACCATCAGGTCTACTTCATTCTCACTATCCA 6 0.15 No Hit ACTAAGGTGACCGACAACATGGTTAGGAGGATAAGGTGCCCGATCACGAA 6 0.15 No Hit CCAAGAGTGATTCGATAACTCCAATCTTAGAAGATACCTTTGAGCCACCA 6 0.15 No Hit GTGTAACCCGTCAGGATTCCCACACAAATAATTAGAGATGAACCTTGACC 6 0.15 No Hit CAAAAACACAATGAGTGCAATCTGAGGAAGGTACGCTTCCAGCACCGTCT 6 0.15 No Hit CCTGCCTGGGTCATCACTAATTCAATATCCTTTGGCTCCACTCCGGTTTC 6 0.15 No Hit GGGGATTAATAGTCCAGTAAGAATGTTCGTTTTCCAGTTCGTTCGATCCA 6 0.15 No Hit AAGAAAGATGGTCATCAATCCAAAGGCATAGCTCAGCCATCCCCTCCAAA 6 0.15 No Hit CCCGGCGATTAAGAAATTCCATTTATCAGCCAAATAGACGACCCCAATTC 6 0.15 No Hit CAAAGATACATTCTCTTGCCGATTACAGGATATGAAACCCGCAAACACCA 6 0.15 No Hit ACAGATGTTGTCAAGAATAAGATCATCCAGGTTGTGGAACAAAGGTACCT 6 0.15 No Hit GCTGCAAATAACTGGCAAGAAAAGCATAAGAGAGGAATCATAGAATAGAG 6 0.15 No Hit CTCCTTTTCAAGTGAATCAGATGTTTGCGAAGATATGACAGTAATGCTTA 6 0.15 No Hit GCTGCCAGTCTTGATGGGCTTGGTTTGGTTTTTTGTAGATAGGCTAGGGC 6 0.15 No Hit GTTACATCGTTGAATGCCAAGCTCAACACCTTCAAGTTTTTCAATCCAGA 6 0.15 No Hit CTCCACAGCCTCCTCAGAAACAGCAGTCGCTGGCTTCTCCTCTTCTTTTA 6 0.15 No Hit TCCTGTGCATAGCTTAAGGGAACAAGAGGGCTTTAATCCGTAAGTTGGCA 6 0.15 No Hit GCAGCAAGCATTGATACATAGGGGAAAGAAACAAAGAGAAGTGGGGGAGG 6 0.15 No Hit GTAGGTGTAGGAAGTGCAAATGTGAACTTTGCCACATTATGGCTCAACTG 6 0.15 No Hit ATCGGCCGTATGCGGCGGTTGTTCTGTGATCTACTAGCGCCAATCTATAT 6 0.15 No Hit CTCCAGATTTGTAACCATAATAGAAGGCCGGGAACGAATTAGCAGAGCAA 6 0.15 No Hit GCCTTGTGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGA 6 0.15 No Hit CTTTCGCCCCTATACCCAAGTCAGACGAACGATTTGCACGTCAGTATCGC 6 0.15 No Hit ACCAGGAGCTGGAATCCGGCAAAAGGGACAACAAAGAGAGCCGGCTGGAG 6 0.15 No Hit ACAGGCATGAATTGTCGAAACCTCTCTAGCAAATCAGGATCGTCAATGAA 6 0.15 No Hit GTAAAAGTACAATGCACTACAGTTTTTTGTCTGTCCAACCCTAAAGGTTA 5 0.125 No Hit CCAGAGTTGTAGCAAGGTACTCCAAATACACAGATAAGGCAGACTCAACC 5 0.125 No Hit ATCCTGTGTATGCTAGAAAAGGATGGACAACAATGGAGTTGAGATTCAGC 5 0.125 No Hit TCCTTCTCAGATTCCCAGTCCACGACAGCTTCCTCGTGCTAACTTCTGAA 5 0.125 No Hit GTCTAGTTCTATCTGGTGAAAGCCCTTCTTCCATAGCTTTCCTTTTGTTA 5 0.125 No Hit TGTGAAGCTTTGCTCGTTTTTGCTTCCAATTCTCTGGTTCCGTTGAATTT 5 0.125 No Hit TCATCTTCCAAATACCTCTTGGCCTTGACCAGAGGCAACTTCCTTAGAGC 5 0.125 No Hit GCATAACAGGCATCTTTGGTTGGCCCTGACCTAAGCCAGCCATTGCAAGA 5 0.125 No Hit TCTCAGTCCAGCAATGCTCGTCATCTCCAATTAGATATCTGTTTGGATCA 5 0.125 No Hit CTCGTTTGTCTTTGGGATTGACACAACATCCATGAAACCAGATGGGTAGG 5 0.125 No Hit GGAGTAGTAGTGTGGATGTTGTTGTTAGACACTTCTTTTTGTCTTTAAAT 5 0.125 No Hit CTGCGCAGGACGCCAAATCAACCACTCCCTGATCGATAGTGAACATACTT 5 0.125 No Hit GCTCAGGGCGGGCGGCATAGGTTATTGTGGAGCAGAGGAGGAGGGCTACC 5 0.125 No Hit AGGCATTCATCTAATTTATTTTTGTGAAGTAGAAACGCGGGATTGATCCA 5 0.125 No Hit GTATGACAAAATAACTCCATGCCTTCATCCCCCAGCCTCTTTCTTTCTTT 5 0.125 No Hit CCCTTTTTCTCCGCCTGCTTCATCCATCACGCCGATCACTTCCCTGAGAC 5 0.125 No Hit CCGCCTTCTTTTAACATACTCCTTAAATCATCATCACTCAAATCTTGCAA 5 0.125 No Hit TTATGAGATCATGATATCTTCTGATAGTCACGGAAGGTAGTTGACTGGTA 5 0.125 No Hit CTTCCACCATTGGTACCGCTCGTGGGACAACACAGGGATCATGGCGACCA 5 0.125 No Hit AGGATCCACAGTTATGAATACAGGCACGATATCAAACCCTGCCTTCTCCT 5 0.125 No Hit GGTCCCTGAACCCTGTCTCCCCAACACGCACAGACCGTTTCAGACCTGGT 5 0.125 No Hit TAAATCTAAATCAGCAACTGCCTTGGCAACTGCATACTGAATAACTCCCT 5 0.125 No Hit CCATCAAGTAGAACTCCTTGCCATGAAGATTGGTAACAAGCACTGCCCCA 5 0.125 No Hit GCTCACTCTTGTCATGTTCAAACACATCCTTCTGCTCTGTGATAATCCTG 5 0.125 No Hit CCTCAGCCACATCTTTTGGAGCCTCAGCTACAGGTTCAGGTTCAGCTGGT 5 0.125 No Hit CCTGACTGTCAAATGAAAGCTCAAACCCGTAAAGGTAATCATGGTTCAGT 5 0.125 No Hit TGACAGTTCCTTCACACAGGGGAGAATATGCTGAATAGCATGTTCTGGAC 5 0.125 No Hit GCGTGGGAACCTTCGACACAAGAGGTAATGAGGGGGCGTGTTCCACAGCC 5 0.125 No Hit GTATGGAATTCATATTATAGGAATTAAAATGTAAAAGGCAAGCCAAGAAA 5 0.125 No Hit CTACATTCCTCCTACGGTCTTCAGCAGCCTTCTCCTTTTCCTTCCTCTCA 5 0.125 No Hit ACCGGCAGCAGATGTCTTCTCCAATTTTTCTTTTCCAATCACTACCCTCC 5 0.125 No Hit GGTTTTTCAGGTCTCTCTTATTCGAAGTCAAATGAAGAACTGTGTGGTGA 5 0.125 No Hit GTTCTCTATAACTAAAACCTAGCTACACTGCCTTTTCATCTTGCTTTGCA 5 0.125 No Hit CTTCTTCTCAGCAACAGGTGTATGAGATTCTATGGGCAAGTCAAAGTCAT 5 0.125 No Hit GGTTGAATCCCATGTGCGAGACATTCTCATTATATGTTGCATTGTCTGAA 5 0.125 No Hit CCCATCATTGCTTTTTGCATGGCTCAAATCAGCCAACTTCTTAATGGAAG 5 0.125 No Hit CGGCCTAACAAGGAAGTCACCTTATTTTCGACACTAGTCTTTGATGCCTT 5 0.125 No Hit GGACTGCTTGGACTAGAAGTGCCATCACTATCCAAGTTACCATCAACTTG 5 0.125 No Hit GGAGGTGCAGGGAGTAACAACTGGTTTAGTGTTTGAGAATTTGAAGTTGA 5 0.125 No Hit CACAAAGAGCTAAGGTTATAATGGATTGAAAACAAAAGGTGTAAGGAATT 5 0.125 No Hit GTTTAGCTTTGGGATCTGATAAGCAAGTCAATAAGTAGACGTTGAAGAAG 5 0.125 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0375 0.0 0.0 0.0 0.0 56-57 0.075 0.0 0.0 0.0 0.0 58-59 0.075 0.0 0.0 0.0 0.0 60-61 0.075 0.0 0.0 0.0 0.0 62-63 0.075 0.0 0.0 0.0 0.0 64-65 0.1 0.0 0.0 0.0 0.0 66-67 0.1 0.0 0.0 0.0 0.0 68-69 0.1 0.0 0.0 0.0 0.0 70-71 0.1 0.0 0.0 0.0 0.0 72-73 0.1125 0.0 0.0 0.0 0.0 74-75 0.125 0.0 0.0 0.0 0.0 76-77 0.125 0.0 0.0 0.0 0.0 78-79 0.1375 0.0 0.0 0.0 0.0 80-81 0.15 0.0 0.0 0.0 0.0 82-83 0.1875 0.0 0.0 0.0 0.0 84-85 0.275 0.0 0.0 0.0 0.0 86-87 0.3125 0.0 0.0 0.0 0.0 88-89 0.3375 0.0 0.0 0.0 0.0 90-91 0.35 0.0 0.0 0.0 0.0 92-93 0.4125 0.0 0.0 0.0 0.0 94-95 0.5375 0.0 0.0 0.0 0.0 96-97 0.5874999999999999 0.0 0.0 0.0 0.0 98-99 0.775 0.0 0.0 0.0 0.0 100-101 0.8875 0.0 0.0 0.0 0.0 102-103 1.0125 0.0 0.0 0.0 0.0 104-105 1.15 0.0 0.0 0.0 0.0 106-107 1.375 0.0 0.0 0.0 0.0 108-109 1.575 0.0 0.0 0.0 0.0 110-111 1.9375 0.0 0.0 0.0 0.0 112-113 2.2375 0.0 0.0 0.0 0.0 114-115 2.575 0.0 0.0 0.0 0.0 116-117 2.975 0.0 0.0 0.0 0.0 118-119 3.275 0.0 0.0 0.0 0.0 120-121 3.675 0.0 0.0 0.0 0.0 122-123 4.0 0.0 0.0 0.0 0.0 124-125 4.35 0.0 0.0 0.0 0.0 126-127 4.9125 0.0 0.0 0.0 0.0 128-129 5.475 0.0 0.0 0.0 0.0 130-131 6.0625 0.0 0.0 0.0 0.0 132-133 6.699999999999999 0.0 0.0 0.0 0.0 134-135 6.925000000000001 0.0 0.0 0.0 0.0 136-137 7.475 0.0 0.0 0.0 0.0 138-139 8.15 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ACTGAAA 10 0.006830828 145.0 145 >>END_MODULE SRR26075384 read2 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR26075384_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.193 37.0 37.0 37.0 37.0 37.0 2 36.2655 37.0 37.0 37.0 37.0 37.0 3 36.1875 37.0 37.0 37.0 37.0 37.0 4 36.233 37.0 37.0 37.0 37.0 37.0 5 36.2765 37.0 37.0 37.0 37.0 37.0 6 36.098 37.0 37.0 37.0 37.0 37.0 7 36.226 37.0 37.0 37.0 37.0 37.0 8 36.1935 37.0 37.0 37.0 37.0 37.0 9 36.314 37.0 37.0 37.0 37.0 37.0 10-14 36.207100000000004 37.0 37.0 37.0 37.0 37.0 15-19 36.1662 37.0 37.0 37.0 37.0 37.0 20-24 36.1182 37.0 37.0 37.0 37.0 37.0 25-29 35.9867 37.0 37.0 37.0 37.0 37.0 30-34 35.96585 37.0 37.0 37.0 37.0 37.0 35-39 35.88285 37.0 37.0 37.0 37.0 37.0 40-44 35.8854 37.0 37.0 37.0 37.0 37.0 45-49 35.821 37.0 37.0 37.0 37.0 37.0 50-54 35.684000000000005 37.0 37.0 37.0 37.0 37.0 55-59 35.6715 37.0 37.0 37.0 37.0 37.0 60-64 35.7462 37.0 37.0 37.0 37.0 37.0 65-69 35.6806 37.0 37.0 37.0 37.0 37.0 70-74 35.5695 37.0 37.0 37.0 37.0 37.0 75-79 35.49159999999999 37.0 37.0 37.0 37.0 37.0 80-84 35.5867 37.0 37.0 37.0 37.0 37.0 85-89 35.5612 37.0 37.0 37.0 37.0 37.0 90-94 35.40825 37.0 37.0 37.0 37.0 37.0 95-99 35.564350000000005 37.0 37.0 37.0 37.0 37.0 100-104 35.33055 37.0 37.0 37.0 37.0 37.0 105-109 35.356199999999994 37.0 37.0 37.0 37.0 37.0 110-114 35.2752 37.0 37.0 37.0 32.2 37.0 115-119 35.30695 37.0 37.0 37.0 37.0 37.0 120-124 35.123200000000004 37.0 37.0 37.0 27.4 37.0 125-129 35.1481 37.0 37.0 37.0 27.4 37.0 130-134 35.014149999999994 37.0 37.0 37.0 27.4 37.0 135-139 34.9363 37.0 37.0 37.0 27.4 37.0 140-144 34.984249999999996 37.0 37.0 37.0 25.0 37.0 145-149 34.88395 37.0 37.0 37.0 25.0 37.0 150-151 34.364125 37.0 37.0 37.0 25.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.0 1101 2 0.0 1101 3 0.0 1101 4 0.0 1101 5 0.0 1101 6 0.0 1101 7 0.0 1101 8 0.0 1101 9 0.0 1101 10-14 0.0 1101 15-19 0.0 1101 20-24 0.0 1101 25-29 0.0 1101 30-34 0.0 1101 35-39 0.0 1101 40-44 0.0 1101 45-49 0.0 1101 50-54 0.0 1101 55-59 0.0 1101 60-64 0.0 1101 65-69 0.0 1101 70-74 0.0 1101 75-79 0.0 1101 80-84 0.0 1101 85-89 0.0 1101 90-94 0.0 1101 95-99 0.0 1101 100-104 0.0 1101 105-109 0.0 1101 110-114 0.0 1101 115-119 0.0 1101 120-124 0.0 1101 125-129 0.0 1101 130-134 0.0 1101 135-139 0.0 1101 140-144 0.0 1101 145-149 0.0 1101 150-151 0.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 13 5.0 14 4.0 15 2.0 16 3.0 17 8.0 18 3.0 19 1.0 20 7.0 21 7.0 22 7.0 23 14.0 24 18.0 25 12.0 26 12.0 27 15.0 28 17.0 29 21.0 30 24.0 31 53.0 32 57.0 33 102.0 34 201.0 35 739.0 36 2490.0 37 178.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 41.070535267633815 21.810905452726363 11.930965482741371 25.18759379689845 2 27.575 25.275 27.025 20.125 3 21.55 27.3 29.725 21.425 4 23.325000000000003 34.1 22.25 20.325 5 26.05 35.65 21.65 16.650000000000002 6 21.875 37.95 22.975 17.2 7 21.224999999999998 22.575 37.025000000000006 19.175 8 22.650000000000002 23.625 28.7 25.025 9 23.775 25.974999999999998 27.925 22.325 10-14 24.6 28.549999999999997 25.805 21.044999999999998 15-19 23.905 28.625 26.405 21.065 20-24 24.32 29.37 25.345000000000002 20.965 25-29 23.724999999999998 29.615000000000002 25.900000000000002 20.76 30-34 24.311215560778038 28.07140357017851 27.006350317515874 20.611030551527577 35-39 24.34621731086554 27.67138356917846 27.151357567878392 20.831041552077604 40-44 23.385 27.435 27.925 21.255 45-49 24.759999999999998 28.355000000000004 26.125 20.76 50-54 23.26 28.689999999999998 27.169999999999998 20.880000000000003 55-59 23.82 28.115000000000002 26.215 21.85 60-64 23.875 28.46 26.865 20.8 65-69 24.425 28.96 25.900000000000002 20.715 70-74 23.575 29.049999999999997 25.955000000000002 21.42 75-79 23.452345234523452 29.127912791279126 27.237723772377237 20.18201820182018 80-84 23.62 27.689999999999998 26.534999999999997 22.155 85-89 24.45 29.310000000000002 26.240000000000002 20.0 90-94 24.526226311315565 29.016450822541128 26.531326566328318 19.92599629981499 95-99 24.57122856142807 28.22641132056603 26.676333816690835 20.526026301315063 100-104 25.02125106255313 28.651432571628582 25.711285564278214 20.616030801540077 105-109 24.154999999999998 29.425 26.31 20.11 110-114 24.485 29.044999999999998 26.284999999999997 20.185 115-119 25.11125556277814 28.71643582179109 26.326316315815788 19.84599229961498 120-124 24.90498099619924 28.960792158431687 25.41508301660332 20.719143828765755 125-129 24.957495749574957 30.028002800280028 26.172617261726174 18.84188418841884 130-134 25.526276313815693 28.871443572178606 26.136306815340767 19.46597329866493 135-139 25.86534613845538 29.226690676270508 26.185474189675872 18.722488995598237 140-144 25.914252839061486 28.705788183500925 25.46400520286157 19.915953774576018 145-149 25.321458948316405 28.21834192224946 26.422174413368687 20.038024716065443 150-151 25.753595997498437 28.367729831144466 27.229518449030643 18.649155722326455 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.5 4 1.0 5 0.5 6 0.5 7 1.0 8 1.0 9 0.5 10 1.0 11 2.5 12 2.0 13 1.5 14 4.0 15 4.0 16 1.0 17 0.0 18 0.5 19 1.5 20 1.5 21 1.0 22 0.5 23 0.5 24 1.0 25 4.0 26 4.0 27 3.5 28 6.5 29 6.0 30 11.5 31 15.5 32 15.5 33 19.5 34 36.0 35 58.0 36 75.0 37 90.0 38 126.0 39 181.0 40 196.5 41 212.5 42 238.5 43 254.0 44 273.0 45 273.0 46 264.0 47 252.5 48 219.5 49 187.0 50 163.0 51 130.5 52 131.0 53 115.0 54 72.5 55 56.5 56 44.5 57 38.5 58 31.5 59 27.0 60 25.5 61 22.5 62 17.0 63 9.0 64 4.5 65 4.0 66 4.5 67 4.0 68 4.5 69 2.0 70 3.0 71 3.0 72 2.5 73 3.0 74 1.0 75 1.0 76 1.0 77 1.0 78 1.0 79 1.0 80 2.0 81 1.0 82 0.5 83 0.5 84 1.0 85 2.0 86 1.0 87 0.0 88 0.0 89 0.5 90 0.5 91 0.5 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.5 99 0.5 100 7.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.05 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.005 35-39 0.005 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.01 80-84 0.0 85-89 0.0 90-94 0.005 95-99 0.005 100-104 0.005 105-109 0.0 110-114 0.0 115-119 0.005 120-124 0.02 125-129 0.01 130-134 0.005 135-139 0.04 140-144 0.055 145-149 0.065 150-151 0.0625 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 58.3 #Duplication Level Percentage of deduplicated Percentage of total 1 61.106346483704975 35.625 2 21.7409948542024 25.35 3 9.519725557461406 16.650000000000002 4 4.159519725557462 9.700000000000001 5 1.5437392795883362 4.5 6 0.9862778730703259 3.45 7 0.42881646655231564 1.7500000000000002 8 0.1286449399656947 0.6 9 0.21440823327615782 1.125 >10 0.17152658662092624 1.25 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG 19 0.475 No Hit CCGGTCGCCTCGTCCCTTCTACCGGCGATGCGCTCCTGGCCTTAACTGGC 11 0.27499999999999997 No Hit CTCAACACTCTCACAAGTCATTCATTATCCGTGCCCAAAAGTTCAAAGCG 10 0.25 No Hit GACACGCAAAGCTGCAGGAGAAGAGCCCTTGCCTGAGGAGGATCCTTCAA 10 0.25 No Hit CGAATAATGAAGGAACCCCGTCAGCCTAAAAGCAAATTGGTTGGGGCAGT 9 0.22499999999999998 No Hit AAGGATTAGACTTGAAGAGGTACATGGGAAGATGGTACGAAATTGCTTCA 9 0.22499999999999998 No Hit CGAAGATGCAGACTTCCTTCAATGTGAAGTGTGAACATTGTCCTGTAAGT 9 0.22499999999999998 No Hit AGCTGGTTGTGGTTACAAGTTTGAGATTCACCCAGGGGAAGTTGATAAGG 9 0.22499999999999998 No Hit GGTATCATCATCCTGCATGTCATCAACTGATAGTGAATACTCAAGTCCAA 9 0.22499999999999998 No Hit CATCATTTACACAGTTGACGGCTCAACAGCTTAGAAATGTGCACATCTCA 8 0.2 No Hit GAACGGATCGAGTTTTAAATGGAGAAATGTCGTTGATGATGGATCGCGAC 8 0.2 No Hit GTGATTACTCCTACACTGGTCAAGGCCTTCCTGCCCCTGATAACTTTCTC 8 0.2 No Hit AGAACACAAATCGTGGTAACAATTTGGTCACAGAAGGTCACAATGTTTTC 7 0.17500000000000002 No Hit GAGACTTTGATTTGTTAAGGGAAAGGTACACGAGGGAATTGTTCTAATCA 7 0.17500000000000002 No Hit GTTCATTCCTGAAAGCATTGGCAAGGAAATTGAGAAGGCAACTTCTAGCA 7 0.17500000000000002 No Hit GTCCAAGGCGAGTGAGGTCATTGATACTATCAAACACATTTCTGGAGACG 7 0.17500000000000002 No Hit TGGAAATCATGTAATATCAACCAATGATTACAACTTCTTTTGTTTCATGA 7 0.17500000000000002 No Hit ATCAGATTAGCACAAGACTTTTGTAATTCTCAACGGCCAACCATGGAAAC 7 0.17500000000000002 No Hit CGAGCAATGGTGAACAGGAAGGCTACAAGGATATCCATAGCTATGGCACA 7 0.17500000000000002 No Hit CGGACAACGGTGTCACCATCACCATCGGAGACAAGGATTTTGTCGAAGTC 7 0.17500000000000002 No Hit AGCTGCCCACCATTTTCAGTGCCAAACTACATGAGTCCTACAGTTTCAGC 7 0.17500000000000002 No Hit CATTGATTGGCCGCTCGCTCTTGTTGAACCAACTCAACAGCAAAGCTGTC 7 0.17500000000000002 No Hit CCCACCCCCACAGGTTCAACAACCAACAAGCCAGGAGTATGGCCAGCCTG 6 0.15 No Hit GATGCATTTGGCACTGCTCACAGAGCCCATGCATCCACTGAGGGCGTGGC 6 0.15 No Hit GGAGAGATGAGTTCATTGGAGAAGGTGGTTAAGGAATCTCAAAACAGCTA 6 0.15 No Hit GGAAAGAAGGCTTGGATGGCCATGAGAAGATTAAGAGTTATATGTGTGTC 6 0.15 No Hit CAGCAGAGCAGTTCAAGGCTCCTGATCTCAGTCATGTCATTTCAAAACCT 6 0.15 No Hit GTCAAAAGAGCAAGATCTTCTTCATTGCATGGTCTCCTTCAACCTCTCGA 6 0.15 No Hit GCTTCAAACCGATCGATTTCTTGTTAGAAGAAGAATGTTGGAAGTGGAAA 6 0.15 No Hit GGATGATACTGACATACCTAGTGGCAGCAGAGGCAGTAATAGCGGTACTC 6 0.15 No Hit GAGAATGGGAAGTGCAGCCGCGCAAATTCCGACGAGCTTTGGACATGAGC 6 0.15 No Hit ACTATGTTGGGTATGCTGTTCGATTTCTTAAAGGACTGAATATTCGGTGG 6 0.15 No Hit ATGAGATCTACTGCTTGTTTGAGGGAGCTCTAGACAACTTGGGTAGCCTA 6 0.15 No Hit GGAATGGTGGATGAGGAGGAGACAGTTGCCTTCTCGTTTGAGACAGAGAG 6 0.15 No Hit TGGTGTTCATCCAGATAAAAGTTGGGAATCCTGGTGGGAAAAGGAGCAGG 6 0.15 No Hit ATGGATTTAGAATTCTTGCACACACTTGATGTTCAGATTCTTGGGGCTGT 6 0.15 No Hit TAGCAATGGAAGCATTTGCCTGGACATTTTGAAGGAGCAATGGAGCCCTG 6 0.15 No Hit GGGGTGCAATATTACCACTGCATGTTTAGATTCCATTTCAGCTCTTGCAA 6 0.15 No Hit GAAAAGAAGAAAGGTTACTTGGCGATGAGGACGGATCAAGAAATGGCAAG 6 0.15 No Hit AAAATAGAAAAAAAAATCATTCGAATTTTGTAAAAGCCAGTCAGCCAATA 6 0.15 No Hit ACCAAACATGCACAGCGGTCAAACAGTATGTCCCAAGGGGACTTAAGCGC 6 0.15 No Hit GAGATTGAGCCGGTCACGGCGGAGGGAGCAGTATTATCTGGACTTGAAGA 6 0.15 No Hit CTCTTACCGACTACAATGGGTCAGTGAACGTAAGGTACTTCTTTCAAGGC 6 0.15 No Hit TGTGACAAAGACCGTTCTCCAAAAAAGGTTCTTGGAGAGGGTAGTCTTAG 6 0.15 No Hit CCCTAACTTAATGGACGGGAGGTATCCCAATAGGAGGTTTCCTCCTATGG 6 0.15 No Hit CAACACCTTCTCTTGTCCACAGACAAAAAATGGCATCTTCACAAAACCAA 5 0.125 No Hit GCTCCTTATCACATCAGCAGTCTGGAAAGCTTGTGTTTGGTTCTACGTCA 5 0.125 No Hit GAAGCACTTGAAGAGGCTTAATGCTCCAAAACATTGGATGCTTGATAAAC 5 0.125 No Hit ATTTTATGAGACACTGTATGAGCAGATTCCCGAAAGTGAAATGGCACAAT 5 0.125 No Hit TAGGCTTAAACCTCACATGATGAAAACCAAAATTAAGAACGAAAAACAAG 5 0.125 No Hit TCCAAATTGGTGTGCTAAGCATATCAAGGCAGTGATTAATATTGGTGGAC 5 0.125 No Hit GACACCTGCTGATGCCTTGCAGGTTGACTCTGTAGCAAGCGAGAAAAATA 5 0.125 No Hit CATAATTTACTCATCCTGCTTAATTCCCAAAAGACCACTACTCTTCCTCT 5 0.125 No Hit GATTAAAGAAATGGCAAATGCAGCCAAAGAGAAGGTTAGCGAAATGGCAA 5 0.125 No Hit GGAGTCTGACATGTGTGCGAGTCAACGGGCGAGTAAACCCGTAAGGCGCA 5 0.125 No Hit CTCTCTCTCACCTTTACTAGCCACCTCTGTTATCTTTATAGCTATCTTCT 5 0.125 No Hit CAGGAAGCAAGTTCGAGAGCCTACAATAAGTTGGAATGCCACCTACGAGA 5 0.125 No Hit GCGAGGATAAACCCTTGCCGGCGACGGTCTTCGAAGATATTCAAAATTGA 5 0.125 No Hit TAAGAAATGGGTTGATCATGCAACTTTTTGGATCGCACTTACTTTGGGAA 5 0.125 No Hit GGAGGAAACAAGCAACCTTCACTTAGCAGAGGAAAGAGGCATCAATCTTC 5 0.125 No Hit GGAATTGACTTCATCCATGAAGTGGAGCAAAGCTATAAGTCTGTTTGAAA 5 0.125 No Hit GTCTTGGCGTGTACGCTACATGGTTGCAAATCAACTGTATGAACTTTGTG 5 0.125 No Hit ACACTGATGCTCGTCAAAGTACTGAAAATAAGCCTCTTCTTGCTTGTGGT 5 0.125 No Hit GACAAAGTTTTGAGTACTTGCCATTTGGATCTGGTCGAAGAATTTGTCCT 5 0.125 No Hit TGACAAAAAATACCAGTGCAGTCTCCTTGGTTCTCCGAGGTGCAAACGAT 5 0.125 No Hit ACTGTATCGTCCTCCCCTCTTCCATGGCTTCCTCCTCCTCCTACTTTCAC 5 0.125 No Hit CAGACATGAGTCATCACAGGTTCACAACAGCATGGCTAGGTTTGAGCGGC 5 0.125 No Hit ACCAAATCAACGTATCTCCAGCTTCGACAAGGCAGACGGTGCAGCCACCA 5 0.125 No Hit CGGCAGTATGTTGTTTGTTTTATTGTGGCTCTGACCATATTGTTTTATAT 5 0.125 No Hit AGCAAAACTCAGAGAGAGAGAGAGGGAGAGAGAGGGGGGGAGAAATTTAT 5 0.125 No Hit GTTCTACACAGATGAACATGGAAGAATACGAACAAGAACAAATCGATCTG 5 0.125 No Hit GGAAAGTCTTACAGAGGAGGTGTGCCTCTCTTTTGCAATCGTTCTCTTTT 5 0.125 No Hit GTTGCAATCACCGATTGACGAGGTGGACCCTTTCATTTTCTTTGTGGACA 5 0.125 No Hit GCCTGATCAATTCGATATGTACTTAACAATTCATACTTCAAGATCTCAGG 5 0.125 No Hit GAACAAATTGAGAAATTCATAAAAGACTATGCTCATGTCGCCGATGTAAT 5 0.125 No Hit CTTTTGTCTAAACCTAAAACAACACTTTTGCCCAAGCTTGAGTTTTTCAA 5 0.125 No Hit ACAAAGGTGGACGTGCTTTCTGAGAAACTAGTTGCTAGCTCTGAAGCTAC 5 0.125 No Hit GTTCACACCATCACCATTACCATTAGTCTCCTCCCTCACCATCTATAAAT 5 0.125 No Hit AAGTAAGCCGATGCGTGAGAAAGTCACAAGAAATGATGTGACCCAGTGTT 5 0.125 No Hit GTTCACCAATTTGAATGAAGATTTCCACCGTTATTTGACATCCTTAAATA 5 0.125 No Hit ATTCAATCATTAAAGAGGCGAAGAGAGAAGAAAACATAAAAAGAAACAAT 5 0.125 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0375 0.0 0.0 0.0 0.0 56-57 0.075 0.0 0.0 0.0 0.0 58-59 0.075 0.0 0.0 0.0 0.0 60-61 0.075 0.0 0.0 0.0 0.0 62-63 0.075 0.0 0.0 0.0 0.0 64-65 0.1 0.0 0.0 0.0 0.0 66-67 0.1 0.0 0.0 0.0 0.0 68-69 0.1 0.0 0.0 0.0 0.0 70-71 0.1 0.0 0.0 0.0 0.0 72-73 0.1125 0.0 0.0 0.0 0.0 74-75 0.15 0.0 0.0 0.0 0.0 76-77 0.15 0.0 0.0 0.0 0.0 78-79 0.16249999999999998 0.0 0.0 0.0 0.0 80-81 0.175 0.0 0.0 0.0 0.0 82-83 0.2125 0.0 0.0 0.0 0.0 84-85 0.3 0.0 0.0 0.0 0.0 86-87 0.3375 0.0 0.0 0.0 0.0 88-89 0.3625 0.0 0.0 0.0 0.0 90-91 0.375 0.0 0.0 0.0 0.0 92-93 0.4375 0.0 0.0 0.0 0.0 94-95 0.5625 0.0 0.0 0.0 0.0 96-97 0.6125 0.0 0.0 0.0 0.0 98-99 0.8 0.0 0.0 0.0 0.0 100-101 0.9375 0.0 0.0 0.0 0.0 102-103 1.0750000000000002 0.0 0.0 0.0 0.0 104-105 1.2374999999999998 0.0 0.0 0.0 0.0 106-107 1.475 0.0 0.0 0.0 0.0 108-109 1.675 0.0 0.0 0.0 0.0 110-111 2.0375 0.0 0.0 0.0 0.0 112-113 2.3 0.0 0.0 0.0 0.0 114-115 2.7 0.0 0.0 0.0 0.0 116-117 3.1125 0.0 0.0 0.0 0.0 118-119 3.425 0.0 0.0 0.0 0.0 120-121 3.85 0.0 0.0 0.0 0.0 122-123 4.175000000000001 0.0 0.0 0.0 0.0 124-125 4.55 0.0 0.0 0.0 0.0 126-127 5.1 0.0 0.0 0.0 0.0 128-129 5.675000000000001 0.0 0.0 0.0 0.0 130-131 6.2375 0.0 0.0 0.0 0.0 132-133 6.85 0.0 0.0 0.0 0.0 134-135 7.074999999999999 0.0 0.0 0.0 0.0 136-137 7.7375 0.0 0.0 0.0 0.0 138-139 8.375 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content pass >>END_MODULE Read 998395 spots for SRR26075384.sra Written 998395 spots for SRR26075384.sra Read 998395 spots for SRR26075384.sra Written 998395 spots for SRR26075384.sra Read 998395 spots for SRR26075384.sra Written 998395 spots for SRR26075384.sra Read 998395 spots for SRR26075384.sra Written 998395 spots for SRR26075384.sra Read 998395 spots for SRR26075384.sra Written 998395 spots for SRR26075384.sra Read 998395 spots for SRR26075384.sra Written 998395 spots for SRR26075384.sra Read 998395 spots for SRR26075384.sra Written 998395 spots for SRR26075384.sra Read 998395 spots for SRR26075384.sra Written 998395 spots for SRR26075384.sra Read 998395 spots for SRR26075384.sra Written 998395 spots for SRR26075384.sra Read 998395 spots for SRR26075384.sra Written 998395 spots for SRR26075384.sra Read 998395 spots for SRR26075384.sra Written 998395 spots for SRR26075384.sra Read 998395 spots for SRR26075384.sra Written 998395 spots for SRR26075384.sra Read 998395 spots for SRR26075384.sra Written 998395 spots for SRR26075384.sra Read 998395 spots for SRR26075384.sra Written 998395 spots for SRR26075384.sra Read 998395 spots for SRR26075384.sra Written 998395 spots for SRR26075384.sra Read 998408 spots for SRR26075384.sra Written 998408 spots for SRR26075384.sra Read 998395 spots for SRR26075384.sra Written 998395 spots for SRR26075384.sra Read 998395 spots for SRR26075384.sra Written 998395 spots for SRR26075384.sra Read 998395 spots for SRR26075384.sra Written 998395 spots for SRR26075384.sra Read 998395 spots for SRR26075384.sra Written 998395 spots for SRR26075384.sra SRR ids: ['SRR26075384.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_610pvjrm SRR26075384.sra spots: 19967913 blocks: [[1, 998395], [998396, 1996790], [1996791, 2995185], [2995186, 3993580], [3993581, 4991975], [4991976, 5990370], [5990371, 6988765], [6988766, 7987160], [7987161, 8985555], [8985556, 9983950], [9983951, 10982345], [10982346, 11980740], [11980741, 12979135], [12979136, 13977530], [13977531, 14975925], [14975926, 15974320], [15974321, 16972715], [16972716, 17971110], [17971111, 18969505], [18969506, 19967913]] SRR26075384 file size 7369193 SRR26075384 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o SRR26075384 SRR26075384_1.fastq SRR26075384_2.fastq Input file: SRR26075384_1.fastq Paired file: SRR26075384_2.fastq trimmed: SRR26075384-trimmed-pair1.fastq, SRR26075384-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Wed Feb 12 03:24:37 2025 >> started Wed Feb 12 03:25:01 2025 >> done (23.819s) 19967913 read pairs processed; of these: 75 ( 0.00%) short read pairs filtered out after trimming by size control 23705 ( 0.12%) empty read pairs filtered out after trimming by size control 19944133 (99.88%) read pairs available; of these: 2719589 (13.64%) trimmed read pairs available after processing 17224544 (86.36%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 18 4 0.00% 19 10 0.00% 20 7 0.00% 21 10 0.00% 22 8 0.00% 23 11 0.00% 24 6 0.00% 25 20 0.00% 26 11 0.00% 27 19 0.00% 28 20 0.00% 29 16 0.00% 30 16 0.00% 31 16 0.00% 32 21 0.00% 33 11 0.00% 34 26 0.00% 35 21 0.00% 36 24 0.00% 37 43 0.00% 38 39 0.00% 39 32 0.00% 40 28 0.00% 41 24 0.00% 42 50 0.00% 43 39 0.00% 44 34 0.00% 45 54 0.00% 46 44 0.00% 47 48 0.00% 48 58 0.00% 49 56 0.00% 50 73 0.00% 51 85 0.00% 52 78 0.00% 53 101 0.00% 54 109 0.00% 55 130 0.00% 56 110 0.00% 57 157 0.00% 58 142 0.00% 59 180 0.00% 60 199 0.00% 61 290 0.00% 62 308 0.00% 63 332 0.00% 64 462 0.00% 65 446 0.00% 66 538 0.00% 67 589 0.00% 68 600 0.00% 69 739 0.00% 70 829 0.00% 71 1061 0.01% 72 1234 0.01% 73 1388 0.01% 74 1516 0.01% 75 1847 0.01% 76 2152 0.01% 77 2357 0.01% 78 2510 0.01% 79 2844 0.01% 80 3314 0.02% 81 3428 0.02% 82 4077 0.02% 83 4438 0.02% 84 5316 0.03% 85 5863 0.03% 86 6670 0.03% 87 7080 0.04% 88 7817 0.04% 89 8379 0.04% 90 8723 0.04% 91 9817 0.05% 92 10589 0.05% 93 11659 0.06% 94 13152 0.07% 95 13830 0.07% 96 14909 0.07% 97 16378 0.08% 98 17070 0.09% 99 17509 0.09% 100 18690 0.09% 101 19825 0.10% 102 20706 0.10% 103 22308 0.11% 104 23036 0.12% 105 24668 0.12% 106 26129 0.13% 107 27324 0.14% 108 28622 0.14% 109 29534 0.15% 110 30319 0.15% 111 31228 0.16% 112 31963 0.16% 113 33422 0.17% 114 35086 0.18% 115 36687 0.18% 116 38045 0.19% 117 39948 0.20% 118 40675 0.20% 119 42097 0.21% 120 42381 0.21% 121 44492 0.22% 122 44921 0.23% 123 46497 0.23% 124 47456 0.24% 125 48801 0.24% 126 51001 0.26% 127 52453 0.26% 128 53404 0.27% 129 55606 0.28% 130 56115 0.28% 131 56454 0.28% 132 57220 0.29% 133 59332 0.30% 134 60216 0.30% 135 61010 0.31% 136 63381 0.32% 137 63786 0.32% 138 64974 0.33% 139 66728 0.33% 140 69356 0.35% 141 68627 0.34% 142 70807 0.36% 143 69915 0.35% 144 71788 0.36% 145 73622 0.37% 146 74528 0.37% 147 74690 0.37% 148 76751 0.38% 149 78682 0.39% 150 80033 0.40% 151 17224544 86.36% 19944133 reads passed initial QC criterion=sequence-density sequence-density=0.19 sequence-density-rank=1 fanout-score=3.03 fanout-score-rank=30 prefix-density=0.26 prefix-fanout=2.2 sequence=CACTTGCAGCCATTCTC criterion=fanout-score sequence-density=0.02 sequence-density-rank=32 fanout-score=189.00 fanout-score-rank=1 prefix-density=0.34 prefix-fanout=11.5 sequence=CATCACCATCCCTGATTGATCTTTGATTCACAACAAGACCAACCAACCGTACAATGTTTACATAACTTGGGATAATCTCACAAGAAAGAAAGATCAATACAAAACTAGTCAGCTCAGGATGTTCCAGGCACGGTAGAACCGTAGAACCATAACAACAAGAGACATATTGCAGATGAGTACTGAAAAACAAAACACAGTACGTATTTACATGGGCAACCTTGGTTGAAGGCAACCTCATCAACGATGCTCGCTCTTCGTCTCTCCACTGTACATCCAGTCATAGACAGTGGGTGTGTTAGGCTGTGGCTTGTCAAAAACATGAGCACCAATATTCTTAGTAGCAAGGTTGCTACCAGGGTGGAACACGCTCCTCCAAACATTGCTACGCGCCGACACTGGGGTTGTAGGGGTCACTGGTGTCGTCGGTGTCCCTGGAGTTCCTGGCATAGTCATGGACCTCTGAAACTTATTAACAGGACTGCTCCCCTCTCCGACGTCAATATCTTTGATG criterion=sequence-density sequence-density=0.33 sequence-density-rank=1 fanout-score=2.49 fanout-score-rank=35 prefix-density=0.35 prefix-fanout=2.4 sequence=TGGTTTTACTAG criterion=fanout-score sequence-density=0.09 sequence-density-rank=20 fanout-score=379.90 fanout-score-rank=1 prefix-density=0.99 prefix-fanout=35.2 sequence=AAGAAGAAGAAA SRR26075384 testing PE reads STAR mapping to Ensembl genome Started job on | Feb 12 03:25:43 Started mapping on | Feb 12 03:25:43 Finished on | Feb 12 03:29:34 Mapping speed, Million of reads per hour | 310.82 Number of input reads | 19944133 Average input read length | 295 UNIQUE READS: Uniquely mapped reads number | 17349268 Uniquely mapped reads % | 86.99% Average mapped length | 294.05 Number of splices: Total | 16263720 Number of splices: Annotated (sjdb) | 15895782 Number of splices: GT/AG | 15955856 Number of splices: GC/AG | 240537 Number of splices: AT/AC | 17379 Number of splices: Non-canonical | 49948 Mismatch rate per base, % | 0.42% Deletion rate per base | 0.03% Deletion average length | 3.27 Insertion rate per base | 0.02% Insertion average length | 2.72 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 480042 % of reads mapped to multiple loci | 2.41% Number of reads mapped to too many loci | 413291 % of reads mapped to too many loci | 2.07% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 7.96% % of reads unmapped: other | 0.57% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 2114823 2114823 2114823 N_multimapping 480042 480042 480042 N_noFeature 422461 17162181 520671 N_ambiguous 187194 1698 97076 UnstrandedReadsAssigned:16739613 PositiveStrandReadsAssigned:185389 NegativeStrandReadsAssigned:16731521 Dataset is classified negative stranded MeadianReadLen=151 20thPercentileLength=151 echo kmer=147 SRR26075384 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,400 [index] number of k-mers: 62,057,036 [index] number of equivalence classes: 130,681 [quant] running in paired-end mode [quant] will process pair 1: SRR26075384-trimmed-pair1.fastq SRR26075384-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 19,944,133 reads, 17,296,446 reads pseudoaligned [quant] estimated average fragment length: 221.241 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,065 rounds 52401 SRR26075384.ke.tsv 34699 SRR26075384.se.tsv 87100 total ==> SRR26075384.ke.tsv <== target_id length eff_length est_counts tpm Potri.005G200100.1.v4.1 2018 1797.76 1673 48.9251 Potri.005G024800.1.v4.1 1035 814.759 1501 96.8542 Potri.004G059700.1.v4.1 961 740.765 10 0.70972 Potri.007G009000.2.v4.1 1416 1195.76 0 0 Potri.003G141000.2.v4.1 2943 2722.76 1067 20.6026 Potri.016G087400.1.v4.1 270 86.1892 1861 1135.17 Potri.015G069301.1.v4.1 564 346.168 0 0 Potri.010G195200.1.v4.1 1773 1552.76 373 12.6291 Potri.012G127500.1.v4.1 977 756.759 5406 375.565 ==> SRR26075384.se.tsv <== Potri.001G166300.v4.1 0 Potri.001G448400.v4.1 241 Potri.001G233950.v4.1 0 Potri.001G122700.v4.1 317 Potri.001G212900.v4.1 0 Potri.001G182400.v4.1 0 Potri.001G256600.v4.1 0 Potri.001G040500.v4.1 0 Potri.001G416900.v4.1 0 Potri.001G452600.v4.1 518 SRR26075384 completed mapping pipeline successfully