Starting /dee2/code/volunteer_pipeline.sh SRR26075385 current disk space = 3051315073024 free memory = 1580025536 SRR26075385 SRAfilesize 2b11fb682c319f9e21c8722be08b16a9 SRR26075385.sra SRR26075385.sra file validated SRR26075385 is paired end SRR26075385 is conventional basespace SRR26075385 read1 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR26075385_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.59075 37.0 37.0 37.0 37.0 37.0 2 36.624 37.0 37.0 37.0 37.0 37.0 3 36.6415 37.0 37.0 37.0 37.0 37.0 4 36.7315 37.0 37.0 37.0 37.0 37.0 5 36.723 37.0 37.0 37.0 37.0 37.0 6 36.688 37.0 37.0 37.0 37.0 37.0 7 36.625 37.0 37.0 37.0 37.0 37.0 8 36.663 37.0 37.0 37.0 37.0 37.0 9 36.623 37.0 37.0 37.0 37.0 37.0 10-14 36.68265 37.0 37.0 37.0 37.0 37.0 15-19 36.62 37.0 37.0 37.0 37.0 37.0 20-24 36.5566 37.0 37.0 37.0 37.0 37.0 25-29 36.4993 37.0 37.0 37.0 37.0 37.0 30-34 36.46319999999999 37.0 37.0 37.0 37.0 37.0 35-39 36.433299999999996 37.0 37.0 37.0 37.0 37.0 40-44 36.4077 37.0 37.0 37.0 37.0 37.0 45-49 36.3373 37.0 37.0 37.0 37.0 37.0 50-54 36.2958 37.0 37.0 37.0 37.0 37.0 55-59 36.2767 37.0 37.0 37.0 37.0 37.0 60-64 36.260099999999994 37.0 37.0 37.0 37.0 37.0 65-69 36.1957 37.0 37.0 37.0 37.0 37.0 70-74 36.200100000000006 37.0 37.0 37.0 37.0 37.0 75-79 36.177499999999995 37.0 37.0 37.0 37.0 37.0 80-84 36.171099999999996 37.0 37.0 37.0 37.0 37.0 85-89 36.041700000000006 37.0 37.0 37.0 37.0 37.0 90-94 36.0246 37.0 37.0 37.0 37.0 37.0 95-99 36.019099999999995 37.0 37.0 37.0 37.0 37.0 100-104 35.9132 37.0 37.0 37.0 37.0 37.0 105-109 35.8268 37.0 37.0 37.0 37.0 37.0 110-114 35.731700000000004 37.0 37.0 37.0 37.0 37.0 115-119 35.688300000000005 37.0 37.0 37.0 37.0 37.0 120-124 35.741699999999994 37.0 37.0 37.0 37.0 37.0 125-129 35.589800000000004 37.0 37.0 37.0 37.0 37.0 130-134 35.415400000000005 37.0 37.0 37.0 37.0 37.0 135-139 35.3378 37.0 37.0 37.0 34.6 37.0 140-144 35.215799999999994 37.0 37.0 37.0 32.2 37.0 145-149 35.2046 37.0 37.0 37.0 29.8 37.0 150-151 35.10225 37.0 37.0 37.0 31.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.0 1101 2 0.0 1101 3 0.0 1101 4 0.0 1101 5 0.0 1101 6 0.0 1101 7 0.0 1101 8 0.0 1101 9 0.0 1101 10-14 0.0 1101 15-19 0.0 1101 20-24 0.0 1101 25-29 0.0 1101 30-34 0.0 1101 35-39 0.0 1101 40-44 0.0 1101 45-49 0.0 1101 50-54 0.0 1101 55-59 0.0 1101 60-64 0.0 1101 65-69 0.0 1101 70-74 0.0 1101 75-79 0.0 1101 80-84 0.0 1101 85-89 0.0 1101 90-94 0.0 1101 95-99 0.0 1101 100-104 0.0 1101 105-109 0.0 1101 110-114 0.0 1101 115-119 0.0 1101 120-124 0.0 1101 125-129 0.0 1101 130-134 0.0 1101 135-139 0.0 1101 140-144 0.0 1101 145-149 0.0 1101 150-151 0.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 19 1.0 20 0.0 21 1.0 22 3.0 23 6.0 24 4.0 25 4.0 26 8.0 27 11.0 28 11.0 29 9.0 30 31.0 31 47.0 32 46.0 33 85.0 34 152.0 35 467.0 36 2910.0 37 204.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 34.968710888610765 14.468085106382977 9.111389236545682 41.45181476846058 2 18.4 13.625000000000002 37.1 30.875000000000004 3 18.975 16.125 27.800000000000004 37.1 4 22.55 22.25 23.3 31.900000000000002 5 25.5 30.425 24.975 19.1 6 23.575 33.0 22.05 21.375 7 16.525000000000002 28.349999999999998 39.25 15.875 8 19.125 25.6 32.4 22.875 9 18.275 23.225 35.6 22.900000000000002 10-14 20.026001300065 29.771488574428723 28.356417820891046 21.84609230461523 15-19 20.09 28.17 27.705000000000002 24.035 20-24 20.605 28.43 27.495000000000005 23.47 25-29 20.645 28.02 28.095 23.24 30-34 20.015 27.815 27.79 24.38 35-39 20.525 28.000000000000004 28.21 23.265 40-44 19.775000000000002 28.99 27.189999999999998 24.044999999999998 45-49 20.59 28.255000000000003 27.195000000000004 23.96 50-54 19.84 27.250000000000004 28.15 24.759999999999998 55-59 20.785 27.96 28.050000000000004 23.205000000000002 60-64 21.365000000000002 28.765 26.68 23.189999999999998 65-69 20.330000000000002 27.965 27.975 23.73 70-74 20.14 28.65 27.515 23.695 75-79 20.095 27.975 27.894999999999996 24.035 80-84 20.599999999999998 27.58 27.735 24.085 85-89 21.0 27.0 28.105000000000004 23.895 90-94 21.07 27.639999999999997 27.515 23.775 95-99 20.955 27.83 27.27 23.945 100-104 20.825 27.855 27.935 23.385 105-109 21.19 27.455000000000002 28.405 22.95 110-114 21.17 26.545 27.875 24.41 115-119 20.93 28.34 27.265 23.465 120-124 21.279999999999998 28.000000000000004 27.175 23.544999999999998 125-129 20.974999999999998 28.09 26.484999999999996 24.45 130-134 21.115000000000002 29.095 26.69 23.1 135-139 20.315 28.975 26.545 24.165 140-144 22.134999999999998 27.215 26.93 23.72 145-149 21.005 28.21 26.715 24.07 150-151 22.2125 27.0875 25.5375 25.162499999999998 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.5 8 1.0 9 0.5 10 0.0 11 0.0 12 0.0 13 0.0 14 0.5 15 0.5 16 0.0 17 0.0 18 0.0 19 0.5 20 0.5 21 0.0 22 0.5 23 1.0 24 1.0 25 2.0 26 3.5 27 5.5 28 8.5 29 14.0 30 13.0 31 11.0 32 18.5 33 32.0 34 53.0 35 59.0 36 76.5 37 93.5 38 114.0 39 137.5 40 182.5 41 212.5 42 226.0 43 268.5 44 289.0 45 293.5 46 285.0 47 256.5 48 228.5 49 207.5 50 188.5 51 161.5 52 154.5 53 125.5 54 63.0 55 51.0 56 40.0 57 23.5 58 19.5 59 21.0 60 14.0 61 4.5 62 9.0 63 9.5 64 4.0 65 3.0 66 2.0 67 2.5 68 2.0 69 0.0 70 0.5 71 0.5 72 0.0 73 1.5 74 1.5 75 0.0 76 0.0 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.125 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.005 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150-151 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 59.85 #Duplication Level Percentage of deduplicated Percentage of total 1 62.48955722639933 37.4 2 21.4703425229741 25.7 3 8.897243107769423 15.975 4 3.8847117794486214 9.3 5 1.6290726817042605 4.875 6 0.9189640768588136 3.3000000000000003 7 0.2506265664160401 1.05 8 0.2506265664160401 1.2 9 0.1670843776106934 0.8999999999999999 >10 0.04177109440267335 0.3 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTTTGATTAGAGTTCCCAAACACAATACAGCGCTGTGGTATAAAGTTTAG 12 0.3 No Hit CTGAGGGACAGATGGTATCATGGTGGTTGTAGCAACAGATCCCACTACAT 9 0.22499999999999998 No Hit TGGCTATTTACGAGAGGCTTCAGTTTTTGCTGATTCTGATGTGAACATAA 9 0.22499999999999998 No Hit AGCTCGGTACGGTGCATTTCACCACCAGTATCATAAGTAACCACCACCTC 9 0.22499999999999998 No Hit GGCTGCCTCAAATTTAAATCTTGAAAATCATAACCCACAGCCTCAAAACT 9 0.22499999999999998 No Hit CCAACAATTTGTTGTACTCTACTCCTTGTAAAGGCTGAAACTAAATCATC 8 0.2 No Hit CACTTGTCTGAGGAGGACTCGACTGACGGGAGTCTTGTTGTACCCATGAA 8 0.2 No Hit GCAATGTTTGACTTAGGGAGATGGCGTAACTGGAAGGTGCTCTCCCAATC 8 0.2 No Hit CTAGACACACTTCTTGAGTTATGCCCCGAATGACTTCTCGAATTTGAAAC 8 0.2 No Hit TTTTTTTTAGAAAAATAGTTCTCAAAATATATTAAAGAAAAACAAACATG 8 0.2 No Hit GACCCAATAACAATCTCTCAACCTGAAGCCTCTCTTGTGAGTCTCTACTC 8 0.2 No Hit GATTGGCTGGTGGTCAGTCCTTGGGTCGGTAAGGACGAGAAGACGAGGCT 7 0.17500000000000002 No Hit CTAGGTTTCAAGTAACAACTCAAAGTAGTAGACTGCCACAGAAAATCGAC 7 0.17500000000000002 No Hit CCTGAATCTTAGATTTGACGTTGTCAATGGTGTCACTGCTCTCAACCTCA 7 0.17500000000000002 No Hit CTTCGTTATCCATGTCATCGGACCCTCGCCATAATTGCATGCTGTCATCT 7 0.17500000000000002 No Hit CTTGGGAACTGATGGAATAAGCTTAAACCTACTGTTGCGGAATTCGTCGT 7 0.17500000000000002 No Hit CTCTGGTAATGAAACCTTATAAGACTGGTAGAGTCCTTGCCTTCATTATT 7 0.17500000000000002 No Hit CCCCCAAGTTTCAAATGTGAGAAACATTGGACAAGAGTCACACGGGAGAT 6 0.15 No Hit GCGTAAAGGGAGAGGGATTGCACACACAAGGCAGGGTATGTTGTGGCGGT 6 0.15 No Hit CTGGTGCAACACCTGCAATGATTGTCTCAGTTGTGGTGTTCTCTGAGAAA 6 0.15 No Hit TACTGATCCTGATCTCCAAGGAAACCATCTATGCCTAGCTTCTTGACACT 6 0.15 No Hit ATGAGTTCCATGTCTTTGCTTGGAACTTTATTTGAAATCACAGCAAACAA 6 0.15 No Hit GCTCCGAGTGTTCTTCACAATCACAGAAATGCTTGGGAAGTGGAGGAACT 6 0.15 No Hit GCTGGGCAGAGTTTTTAGCTGTGGCAGCAGCATCTTGGAGAAGAGGCAAG 6 0.15 No Hit GTCCCCTTGTAGCGTTGAGCTGCCTCGTTGCTGTCGATAACTTCTGTTGC 6 0.15 No Hit CCTTGGAAGGAGGACAAAATAACAAAGGGAGCAGTACGCATATCCTTCCC 6 0.15 No Hit GCCCGCGAGAAGTTTGTGACGATTAGAACCATAACTCTTCTGGTTGTTCT 6 0.15 No Hit ACCACGTTGACGTCTCCGAGCAAACTGTGCCACACTCTCAGCTACATCAG 6 0.15 No Hit CATTAGGATCATTAAAGGACAGCAACACTTGAAGAAACACAATCAGAGTC 6 0.15 No Hit GCCCATAGCCACCACCATCTAGTAACAGAACCATATCCTGCAACCTCTAC 6 0.15 No Hit GCCCTTTCTTTACAGGAGATTATTCTGACAGAAAAGATTATAATTAATGA 6 0.15 No Hit GCCCCTCCTAAATGGGTCCTTGAATATTGCTTGTGGGTATAAGATGACTT 6 0.15 No Hit CTCCAACATATGGCACATACTTGATTACTACAACATGATCAGGGTGTTCA 6 0.15 No Hit CAAGAGGTTCACATCCCCAGAAGACAAGATTAAGGACACCACCTTCTATT 6 0.15 No Hit GCCAGTTGTCGGAGAGTTTCCATTACTTTCTCTGCCTGAAAAGCATCAAG 6 0.15 No Hit CCTAAAAAGCACTGAGCAGCTCCCAAAAATACTTCATTCTTGAGAATAAA 6 0.15 No Hit CTTTTCTCCTGATACCTAGTTCAAAGCAGCAGTTTCTGGAGTTCGTCCTT 6 0.15 No Hit TGATGGAGCATTCCATTCCTGTCTGAGCTCTCTAGTTGTAGAACTGACCA 6 0.15 No Hit CCGGAAGTTCCACATAGAACAGGAAGCAGTGCAAACAGCAAACCAACTGC 6 0.15 No Hit TGGTGCTGCTGCTCCAGTCAGAGCTTGCAGGCTCATTTCGATCCAAACCA 5 0.125 No Hit GCTTTTCGAAAGGCCTCTAGAACATTCAACTCATCATACACATGACTCCC 5 0.125 No Hit CCCTGTCATAGCACTGGTCCTTGACCCACCCCTACCGAAGCTCGCTGTTA 5 0.125 No Hit GGCACCAAAATATATTGGAACTGCACCGGAATCTAAAGCATAAAATAGCT 5 0.125 No Hit GCTGGGTTTTCTCTTTGGCACTGAGAAGTCCCTTAAATCATTCTTGGAAA 5 0.125 No Hit ATCGTGGCTAGCCAAGAGCCTTAGTATACGGTCCAGCATCATGGGTGCAT 5 0.125 No Hit TCTGTTTTTCAGGCGGGGCAGTCACTGACGTGGCTGGCATTGAGCTAGAA 5 0.125 No Hit CTCTTTTTCAGCAAATAACTCAGTGGTTGTCGCACAGGGAGCCACCAAAA 5 0.125 No Hit GATAATGTCAATATGGGCAAGATAACTAACAAAGGAGTGGAATTGGCTGC 5 0.125 No Hit TTTCAGGTCTTGATTCTACTTTAGTGGCAGCTGTGAAGCGTTGCCATATG 5 0.125 No Hit TGGATTTCTTCGAGGACTTGAGAGGAGTCGGTGCATCCAAACATGGGGAG 5 0.125 No Hit CACCGATATCGTCTTGAAGTAGGATGGTGAGGAAATCAGGGTCAGAGTGC 5 0.125 No Hit AGACCAAGAAGAGGCACGGTTAAACGAAGAAGAATAGTTAGCGAACTTCA 5 0.125 No Hit AGCCGCTGCCACTGTACTTCTCATCATCAAGCGGCTCTGCTTTGAGCTTC 5 0.125 No Hit CTAGGCAAAGACTGCAGCCGAGTAATCGCTTTGGCTGCAAGCTTGTATGA 5 0.125 No Hit CGGCATAGTACATCACATATTCACATCGACCCAACATCATACATCAGAAA 5 0.125 No Hit GGCTTCTTTCTTGTTATTTGGGTTTTTATCAGGATGCCATTTCATTGCTA 5 0.125 No Hit GTTCCATGGCGGTTTGGGCGGCCTTCATGAGCTTGGTAGCGAGGCCAAGC 5 0.125 No Hit GGAGAAGGCAGGGCAGGAGCAGGGGCTGGAGAGGGAATAGCAGCAGCTGG 5 0.125 No Hit CAGCGCATAAACAAACTTACACTATAGTTCAAATCATAAAAAGTCTAGAC 5 0.125 No Hit CAAGGCCAAGTCCATCAATAAACCATTTGCTGCTAGTTTTGTCATAAAGG 5 0.125 No Hit ACCCTGGTTGGCATAACTGATTCCCATGTTTGCCCATGCTCGAACATAGT 5 0.125 No Hit GCCAAGTAGTGCAATCACAGCCAAGTGATATTTTCCCATCAAGTGCACTC 5 0.125 No Hit TCCATCTTGGTAACTGGTTGAGCAGTTGGCTCCCTCTCAACCTTGGCCAA 5 0.125 No Hit GTAAGATGTAAATTCTGAAGGATAGTTCTTGCAAAGGACCTCTATAGGAA 5 0.125 No Hit GGGAAAATTGCAAAATTTCTTGGCAATGACTTCGGTGCTTGAAAAGGATC 5 0.125 No Hit CTCTGCTGTACAGTCCTCCATAACGACCAGTATATCCCAAGGAAGGCTCT 5 0.125 No Hit CAACAGCAACCATGTTCTAAATAAAGATAATCCTTGTTCCATACTTAAGA 5 0.125 No Hit GCTCCTTTCTCCAGTTGTATAATTTCTGTTTCTTTGGAGAACTTTGGAAA 5 0.125 No Hit GTGGAATCTTTCATAGAGTACAGGTCTCACATCTTTCTGCTGAGAGAGTT 5 0.125 No Hit CCAGACTTTAGCATCTTCTCAACCTCCTCGAATTGTAGACCTTTGGTTTC 5 0.125 No Hit AGCCTGAGATCGAGCGAGCATCTCCGTCATTGATGGGGTTTGATAAGATT 5 0.125 No Hit TCCCTTTCTGAGACAGGTGTGCGTTTTGGCTGAAGAGAAGCTTTGCCTCC 5 0.125 No Hit GCATTGTTGTCTTAACTTTACCATTTAGCACTCATAGTAGAGTTCAGGAG 5 0.125 No Hit GTGCCGGCATGTCATCTGGACCTTCGAGGGTATGCTTCCAAGGAGCAGAT 5 0.125 No Hit ATCTCGTTATCGGTTCGACCTGGAAGTCTTCCAGCAATGAGAGACCACCG 5 0.125 No Hit CTCAGTGGGAATTTCACAGTGCCTACGAGAGGTTGGGGGGTTTCCGATGG 5 0.125 No Hit CAGCATATTCTGGTGACATATAGCCAAATGTTCCTACAACTCTCCTGGTC 5 0.125 No Hit GCTTGGTATATACACGATGCCTTCTCTCTCTTGCATAAATTACCCAGAAG 5 0.125 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0125 0.0 0.0 0.0 0.0 62-63 0.025 0.0 0.0 0.0 0.0 64-65 0.025 0.0 0.0 0.0 0.0 66-67 0.025 0.0 0.0 0.0 0.0 68-69 0.025 0.0 0.0 0.0 0.0 70-71 0.025 0.0 0.0 0.0 0.0 72-73 0.025 0.0 0.0 0.0 0.0 74-75 0.05 0.0 0.0 0.0 0.0 76-77 0.05 0.0 0.0 0.0 0.0 78-79 0.05 0.0 0.0 0.0 0.0 80-81 0.075 0.0 0.0 0.0 0.0 82-83 0.075 0.0 0.0 0.0 0.0 84-85 0.075 0.0 0.0 0.0 0.0 86-87 0.1 0.0 0.0 0.0 0.0 88-89 0.15 0.0 0.0 0.0 0.0 90-91 0.1875 0.0 0.0 0.0 0.0 92-93 0.23750000000000002 0.0 0.0 0.0 0.0 94-95 0.35 0.0 0.0 0.0 0.0 96-97 0.475 0.0 0.0 0.0 0.0 98-99 0.7250000000000001 0.0 0.0 0.0 0.0 100-101 0.8500000000000001 0.0 0.0 0.0 0.0 102-103 1.025 0.0 0.0 0.0 0.0 104-105 1.2125 0.0 0.0 0.0 0.0 106-107 1.4125 0.0 0.0 0.0 0.0 108-109 1.725 0.0 0.0 0.0 0.0 110-111 2.1 0.0 0.0 0.0 0.0 112-113 2.4375 0.0 0.0 0.0 0.0 114-115 2.6375 0.0 0.0 0.0 0.0 116-117 3.1875 0.0 0.0 0.0 0.0 118-119 3.6375 0.0 0.0 0.0 0.0 120-121 4.0125 0.0 0.0 0.0 0.0 122-123 4.5125 0.0 0.0 0.0 0.0 124-125 5.1 0.0 0.0 0.0 0.0 126-127 5.8875 0.0 0.0 0.0 0.0 128-129 6.3375 0.0 0.0 0.0 0.0 130-131 6.8125 0.0 0.0 0.0 0.0 132-133 7.6 0.0 0.0 0.0 0.0 134-135 8.225 0.0 0.0 0.0 0.0 136-137 9.0 0.0 0.0 0.0 0.0 138-139 9.600000000000001 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CAATGCA 10 0.006830828 145.0 9 >>END_MODULE SRR26075385 read2 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR26075385_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.41675 37.0 37.0 37.0 37.0 37.0 2 36.445 37.0 37.0 37.0 37.0 37.0 3 36.371 37.0 37.0 37.0 37.0 37.0 4 36.4295 37.0 37.0 37.0 37.0 37.0 5 36.3535 37.0 37.0 37.0 37.0 37.0 6 36.3495 37.0 37.0 37.0 37.0 37.0 7 36.365 37.0 37.0 37.0 37.0 37.0 8 36.414 37.0 37.0 37.0 37.0 37.0 9 36.3755 37.0 37.0 37.0 37.0 37.0 10-14 36.320100000000004 37.0 37.0 37.0 37.0 37.0 15-19 36.3472 37.0 37.0 37.0 37.0 37.0 20-24 36.2655 37.0 37.0 37.0 37.0 37.0 25-29 36.2082 37.0 37.0 37.0 37.0 37.0 30-34 36.1163 37.0 37.0 37.0 37.0 37.0 35-39 36.1135 37.0 37.0 37.0 37.0 37.0 40-44 36.084700000000005 37.0 37.0 37.0 37.0 37.0 45-49 36.04729999999999 37.0 37.0 37.0 37.0 37.0 50-54 35.9906 37.0 37.0 37.0 37.0 37.0 55-59 36.0078 37.0 37.0 37.0 37.0 37.0 60-64 36.0631 37.0 37.0 37.0 37.0 37.0 65-69 35.9807 37.0 37.0 37.0 37.0 37.0 70-74 35.9022 37.0 37.0 37.0 37.0 37.0 75-79 35.8827 37.0 37.0 37.0 37.0 37.0 80-84 35.911500000000004 37.0 37.0 37.0 37.0 37.0 85-89 35.819500000000005 37.0 37.0 37.0 37.0 37.0 90-94 35.7269 37.0 37.0 37.0 37.0 37.0 95-99 35.7705 37.0 37.0 37.0 37.0 37.0 100-104 35.6534 37.0 37.0 37.0 37.0 37.0 105-109 35.70380000000001 37.0 37.0 37.0 37.0 37.0 110-114 35.6111 37.0 37.0 37.0 37.0 37.0 115-119 35.615700000000004 37.0 37.0 37.0 37.0 37.0 120-124 35.501549999999995 37.0 37.0 37.0 37.0 37.0 125-129 35.4197 37.0 37.0 37.0 37.0 37.0 130-134 35.4286 37.0 37.0 37.0 37.0 37.0 135-139 35.277550000000005 37.0 37.0 37.0 34.6 37.0 140-144 35.35195 37.0 37.0 37.0 37.0 37.0 145-149 35.26645 37.0 37.0 37.0 32.2 37.0 150-151 34.96075 37.0 37.0 37.0 25.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.0 1101 2 0.0 1101 3 0.0 1101 4 0.0 1101 5 0.0 1101 6 0.0 1101 7 0.0 1101 8 0.0 1101 9 0.0 1101 10-14 0.0 1101 15-19 0.0 1101 20-24 0.0 1101 25-29 0.0 1101 30-34 0.0 1101 35-39 0.0 1101 40-44 0.0 1101 45-49 0.0 1101 50-54 0.0 1101 55-59 0.0 1101 60-64 0.0 1101 65-69 0.0 1101 70-74 0.0 1101 75-79 0.0 1101 80-84 0.0 1101 85-89 0.0 1101 90-94 0.0 1101 95-99 0.0 1101 100-104 0.0 1101 105-109 0.0 1101 110-114 0.0 1101 115-119 0.0 1101 120-124 0.0 1101 125-129 0.0 1101 130-134 0.0 1101 135-139 0.0 1101 140-144 0.0 1101 145-149 0.0 1101 150-151 0.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 12 1.0 13 4.0 14 3.0 15 8.0 16 3.0 17 3.0 18 3.0 19 4.0 20 2.0 21 3.0 22 9.0 23 12.0 24 7.0 25 5.0 26 4.0 27 10.0 28 13.0 29 18.0 30 16.0 31 21.0 32 49.0 33 68.0 34 159.0 35 599.0 36 2752.0 37 224.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 37.609402350587644 20.705176294073517 13.703425856464117 27.981995498874717 2 28.075 25.7 30.375000000000004 15.85 3 21.7 28.199999999999996 32.0 18.099999999999998 4 23.150000000000002 30.775000000000002 25.775 20.3 5 27.125 34.2 23.200000000000003 15.475 6 22.275 37.625 22.85 17.25 7 20.5 23.400000000000002 37.5 18.6 8 21.625 25.775 28.15 24.45 9 23.125 23.674999999999997 30.175 23.025000000000002 10-14 23.71 30.764999999999997 25.380000000000003 20.145 15-19 24.265 28.439999999999998 26.665 20.630000000000003 20-24 23.335 27.755000000000003 27.445000000000004 21.465 25-29 23.405 28.625 26.619999999999997 21.349999999999998 30-34 23.535 28.655 27.029999999999998 20.78 35-39 24.154999999999998 28.115000000000002 27.07 20.66 40-44 23.150000000000002 27.785 28.035 21.029999999999998 45-49 23.35 28.060000000000002 27.375 21.215 50-54 23.04 28.999999999999996 27.139999999999997 20.82 55-59 23.49 28.854999999999997 27.77 19.885 60-64 23.625 28.560000000000002 27.189999999999998 20.625 65-69 24.465 28.215 26.555 20.765 70-74 23.595 27.765 28.235 20.405 75-79 23.98 28.34 26.765 20.915 80-84 23.49 28.084999999999997 27.095000000000002 21.33 85-89 23.53 29.315 26.810000000000002 20.345 90-94 24.035 28.444999999999997 27.425 20.095 95-99 24.02 27.87 28.26 19.85 100-104 23.990000000000002 28.005000000000003 26.995 21.01 105-109 24.169999999999998 28.485 26.135 21.21 110-114 24.349999999999998 28.810000000000002 26.375 20.465 115-119 24.135 28.810000000000002 26.99 20.064999999999998 120-124 25.141257062853146 28.021401070053503 26.71633581679084 20.121006050302515 125-129 24.875 29.035 26.8 19.29 130-134 25.105 28.535 26.740000000000002 19.62 135-139 24.618692803920588 29.19937990698605 26.333950092513874 19.847977196579485 140-144 25.398809821473222 28.70430564584688 26.223933590038506 19.672950942641396 145-149 26.241560390097522 28.30207551887972 26.2015503875969 19.254813703425857 150-151 27.169292323080768 29.182295573893473 24.48112028007002 19.16729182295574 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.5 8 0.5 9 1.0 10 1.0 11 0.5 12 0.5 13 0.5 14 1.0 15 1.0 16 1.0 17 1.0 18 1.5 19 1.5 20 0.5 21 1.5 22 1.5 23 0.5 24 1.0 25 1.5 26 3.5 27 4.0 28 3.5 29 6.5 30 5.5 31 10.0 32 19.5 33 28.0 34 34.0 35 41.0 36 69.5 37 106.0 38 141.5 39 176.5 40 200.0 41 215.0 42 266.5 43 300.0 44 281.5 45 273.0 46 290.5 47 274.5 48 236.5 49 211.0 50 179.0 51 141.5 52 102.0 53 79.5 54 65.5 55 54.0 56 43.0 57 31.0 58 19.5 59 18.0 60 13.5 61 6.5 62 3.5 63 3.0 64 2.0 65 1.5 66 1.5 67 0.5 68 1.0 69 0.5 70 1.0 71 1.0 72 0.5 73 1.0 74 1.0 75 1.5 76 1.0 77 0.0 78 1.0 79 1.0 80 1.0 81 1.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.5 88 1.0 89 1.0 90 0.5 91 0.5 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 1.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.025 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.005 125-129 0.0 130-134 0.0 135-139 0.015 140-144 0.015 145-149 0.025 150-151 0.025 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 60.675000000000004 #Duplication Level Percentage of deduplicated Percentage of total 1 63.78244746600742 38.7 2 20.93119077049856 25.4 3 7.993407498969922 14.549999999999999 4 4.243922538112897 10.299999999999999 5 1.4833127317676145 4.5 6 0.9888751545117428 3.5999999999999996 7 0.12360939431396785 0.525 8 0.288421920065925 1.4000000000000001 9 0.08240626287597858 0.44999999999999996 >10 0.08240626287597858 0.575 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source ATTCAGATTACGAACTGGCTCAAAGGAGTTTGTGAATATTTTGATGCATT 13 0.325 No Hit CCAGCTTGAGCAAATTCAGTTTCTAAGCAAAAGCTTTCCAGGCCCCTTTA 10 0.25 No Hit TTCTGGTATAAGGAAGATCCTTCCGCAATCTGAGATCTGCGATTTTGATT 9 0.22499999999999998 No Hit AATGGGTCTTCCATTTCATCCAAATCGGCACAGCCAAAAGTCCAAGTCCA 9 0.22499999999999998 No Hit GTTTGACAATAAAAGGAGCTCAACCAAAAGTAATTGTGGAGTGGTTGAGG 8 0.2 No Hit CGATGAGGTTGAAAAGAAAGCCAATAATGCTATGGAGCCAGCTTCAAAGC 8 0.2 No Hit TATGAAGTTAGTTTGAACCGGGTCGGGTACTTGACCCGGTTTAAGAACGC 8 0.2 No Hit ACAAGAAGAAAATTAAAGATGCAGTTAAGAAGATGTACGACATTCAGACC 8 0.2 No Hit CGTATTCAGAAGTTCGAGAGAGTACAGCAGGACAGGTCAAGATGAACAAC 8 0.2 No Hit CTGCAGAGTGAGTAGAAGAGAGATGGAGTTCCCAGTGATTAGCATGGAGA 8 0.2 No Hit TTTTGGGCGTGGAAGAAGACCTCACACCCGATTCTGTTGTCTGTGCTGGA 8 0.2 No Hit GTTTTTAATCTGCAAGTTCCTGGTTCAACACACTACAGTATGGTATTTTA 7 0.17500000000000002 No Hit TTACATCATCAACCTTGGGAAGACATGGGATAAACTTCTATTGGCTGCCA 7 0.17500000000000002 No Hit TGTGAACACTAGTCAGGTTACAGTTGGAAAGAGACCAGTAACTGGAATGA 7 0.17500000000000002 No Hit TGATCATGCTCAAGATGATCCCACCAACAAGAAACTGCCCATTTCTTCAA 6 0.15 No Hit CGAGGACACTAACCTTTGTGCCATCCATGCCAAGCGTGTCACTATCATGC 6 0.15 No Hit TTTTGCAGCCATTGATGAGCACTTCGGCCGGGCCAAGAGGCGGCCACCAG 6 0.15 No Hit GTGAGTTACAACCATCTGGGAAACAATGATGGCATGAATCTATCAGCACC 6 0.15 No Hit GAAAAAATTATGGTGTGAGTTGTTTTGATTATTTGATTGGATGAAATCAG 6 0.15 No Hit CTTGGTGCGAGGTTCAAATCTCAAGCCTTCACTTTGCTGACTTCTCTGGT 6 0.15 No Hit GTTTTATTAGCGAGACAAGAATTTTGGCAATGAACATTGAGGATGAGTTG 6 0.15 No Hit CCTGAGGATAAGGATGGCGGTGTGGCTGACCTGGAGGCGGTTGTTAATGT 6 0.15 No Hit CACCAAGCCACTCTCCAACCCAAAGAAGATCCAATCTTCTTGTCTAACAT 6 0.15 No Hit CTTCAACAGCAGTGGTATTGAAGTTCTTGATGGAAAAGAATTCTATTAAT 6 0.15 No Hit GATTTCTTCAAAGGATGGATTTGGCTCGCAAGTATGCCTTTGGGAAGATG 6 0.15 No Hit GAATACGTGAATAATTTGTTGTTCAAGCTCGGTTTGGCCAGCTGTGCTGA 6 0.15 No Hit CCCTATACAGAGCAGTCTCTCTCTCTAGATTCATTGAATCTGAAAGTAAA 6 0.15 No Hit GCAATCTTTCTTTCTCTAACGAAGAAATCAAGCAGAGAAAGAAAAGAAAA 6 0.15 No Hit TGTGTTACGATCACCTCATGTTGATAAAAAGTCCAGGGAACAATTTGAAA 6 0.15 No Hit CAATTCTCTCCATTATGCTAAGGGGTATTAAGTATGTGACATTCTGAGAT 6 0.15 No Hit CAACTTACAAAGTCGTTGAGCTGGATGAGATAAGTGATGGATCTCAACTT 6 0.15 No Hit GGGAGATCTCTTGGAAAATCACCAAGTCTGAGTTCAAGCAATACAAGAAC 6 0.15 No Hit AGAGGCCTGCCAGTGCATTCTTCGTCTTCATGGAGGAGTTCAGAGAGCAG 6 0.15 No Hit GAAGAAGATGATGATGATGATGATGATGAGAGAGATACCGGCGATGAGCC 6 0.15 No Hit TGGAAACTGAGATGGCCCATGCTTGTGGTGGCTGTGTAGTTTGTGCCGCT 6 0.15 No Hit GAGCTTGAGTGCATTCTTCTAAGTAAAAGAAATCCCTTAATTTCATCACA 6 0.15 No Hit GAAATCTTGATTGAAAACCTGATGGATCCTGCTCATGTTACCTATGCACA 6 0.15 No Hit GAAAGAAGAAGCCTGTTCCGTTCTCCCAAAAGTTCCCTAATGCAGACCCC 6 0.15 No Hit GGAATGGGAACTGCGCCGTGGATCGTAAACTCGGAGATATATCCACTGAG 5 0.125 No Hit GACACCATCAGCATTTATCTTTCTGTATGAAAACCAGTTGTTCTTAACAT 5 0.125 No Hit CCTAAACAGCCAAAGTCTATCAAGTCTCCTGCTGCCAAAAGGGCCAAGAA 5 0.125 No Hit CTTGCTAGCCTTAGATGAGAAAACTTTCAAAGTCGTTGCATACAGTGAGA 5 0.125 No Hit CTCTCTGTTTTTCTCTTTCATATTTAATATTTGAGAGAGGGAAAAAATAT 5 0.125 No Hit CATGCTGTGGAGGAGGAAGCTAGAGGAGCAAGCTGATTTGCAGCAAGCCC 5 0.125 No Hit ACTTTGAAGTTTGAAGGGAGAAATGGGAGGAGCAGCAGCAGCAGCAGCAC 5 0.125 No Hit GAAAGATCTCTCCTGCATATCAAAGGATCCATGCCGGATTGTTATTGTTG 5 0.125 No Hit GAGGAGATCTAGCTGGTGCAGCTTCTATCCTTGAAAACTGTCGAATGTTA 5 0.125 No Hit GTGATGATGATGATGAAAAGTTTCATGGTGAAAAGGAGAATTACTGTGAC 5 0.125 No Hit TGTTGGAATAGCTCGTGGGATTTTATATCTTCACCAAGATTCCAGGTTGA 5 0.125 No Hit GTCATCTTATTACCCCAAAGATACTGAATGAAATTGGGAGTGACTTGTCA 5 0.125 No Hit TGCAGCTATATATATACACGAAAACCATAAATAAAATGGCTCGCTCTCTC 5 0.125 No Hit GTCGATTCTGGTCTTGCATTGCCTTCTGTACCAGGATTAAAAACCCTCGC 5 0.125 No Hit GTGAAAGAAAAATTTGACAAGTGCAATAAAGAGAAGTTGTTGGAATTTTG 5 0.125 No Hit CTCCACACTCGAAAGAACCATCTCCAGAACCCGAATCCTTGCACCAAATC 5 0.125 No Hit GGGATCTTTTATAGAAAGTCACATTGGCCAAGTTGATGAAGCAAAAGATG 5 0.125 No Hit ATGTTGTCAAAATGAGTGGGGATCGTTATACTGCTCTTGTACTTAACACC 5 0.125 No Hit GAAAAATCTCATGGCTTCCTCGAAGTCTCACGATCTCGAGATCACAATAA 5 0.125 No Hit AGTTGAGCAAAGCAGAAGAGATGATCTGGAATCCCTTGGTTATGTGCTTA 5 0.125 No Hit GGAGACGATCTTGTCCTGGTATCACATTTGCATTGCAAGTGCTCCATTTG 5 0.125 No Hit GTAATAAGGAAAAAGGAGGCTTTGCAGCAATCAGCACCAGCAGCTGCTAT 5 0.125 No Hit ACCTGAGGATGCTGATGTGCACATAGTACTTGGTGTCCTGTACAATCTGT 5 0.125 No Hit GCTTCTTTGATGCTTCCTTCTCTAAGGAACACTGGAAGTCCTCTTTCTCT 5 0.125 No Hit GATTCAATTGCGAAGGAGATTGATTACATGATGAAAAAAGGATGGATCCC 5 0.125 No Hit CATCACGCTGCCTTGCTGATAGAAACCAGCTGGCTAAGAGTCTCCTCAGC 5 0.125 No Hit ATATTACTTCTACCACATCCTCTCTTGGCCGCATCTCCTTTATGTCGCCG 5 0.125 No Hit AGAGAGTAAGAACATTCTTTTTGGACCCCGAGTGCGAAGTAAAACCGCTA 5 0.125 No Hit AGTTGCCTCTGGTTTCCAGAGATATAATGATGGAGTACTCGAACAAAGTT 5 0.125 No Hit GGTCAAGGACCAGTGCTATGACAGGGAAAATGGAAGATGGGGCGGTGGCC 5 0.125 No Hit GGTTGAATCGTTATGGTCCATTCTTGGTTAACCTGGAAGACTGCACTTTT 5 0.125 No Hit TGTATATGTGAATTACTTTTCTTGTTTAATATCTGGTCCTCTTGTAGCAT 5 0.125 No Hit CCTAACCAAAAAAAACAAAGCAAGCAACCTTTTCTATTATTATTTTTCAA 5 0.125 No Hit GTGAAAGATCAAAGCAAGGTTTTTGAAGTGAATGGAAGGGTCCATTTTCG 5 0.125 No Hit AGGCATTGTACAAGAAGGTTCATGCTGCTATTCGTGCAGATCCAACAACC 5 0.125 No Hit TAGCGTTGGTGGTGGTTATAGGTCTGGAGGGGCCTACGGAAGTAGAGCTG 5 0.125 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0125 0.0 0.0 0.0 0.0 62-63 0.025 0.0 0.0 0.0 0.0 64-65 0.025 0.0 0.0 0.0 0.0 66-67 0.025 0.0 0.0 0.0 0.0 68-69 0.025 0.0 0.0 0.0 0.0 70-71 0.025 0.0 0.0 0.0 0.0 72-73 0.025 0.0 0.0 0.0 0.0 74-75 0.05 0.0 0.0 0.0 0.0 76-77 0.05 0.0 0.0 0.0 0.0 78-79 0.05 0.0 0.0 0.0 0.0 80-81 0.075 0.0 0.0 0.0 0.0 82-83 0.075 0.0 0.0 0.0 0.0 84-85 0.075 0.0 0.0 0.0 0.0 86-87 0.1 0.0 0.0 0.0 0.0 88-89 0.15 0.0 0.0 0.0 0.0 90-91 0.1875 0.0 0.0 0.0 0.0 92-93 0.23750000000000002 0.0 0.0 0.0 0.0 94-95 0.36250000000000004 0.0 0.0 0.0 0.0 96-97 0.525 0.0 0.0 0.0 0.0 98-99 0.8 0.0 0.0 0.0 0.0 100-101 0.9375 0.0 0.0 0.0 0.0 102-103 1.125 0.0 0.0 0.0 0.0 104-105 1.3125 0.0 0.0 0.0 0.0 106-107 1.4875 0.0 0.0 0.0 0.0 108-109 1.825 0.0 0.0 0.0 0.0 110-111 2.2125000000000004 0.0 0.0 0.0 0.0 112-113 2.5875 0.0 0.0 0.0 0.0 114-115 2.7875 0.0 0.0 0.0 0.0 116-117 3.3375 0.0 0.0 0.0 0.0 118-119 3.7874999999999996 0.0 0.0 0.0 0.0 120-121 4.1625 0.0 0.0 0.0 0.0 122-123 4.6875 0.0 0.0 0.0 0.0 124-125 5.3 0.0 0.0 0.0 0.0 126-127 6.0875 0.0 0.0 0.0 0.0 128-129 6.575 0.0 0.0 0.0 0.0 130-131 7.0875 0.0 0.0 0.0 0.0 132-133 7.875 0.0 0.0 0.0 0.0 134-135 8.5125 0.0 0.0 0.0 0.0 136-137 9.3 0.0 0.0 0.0 0.0 138-139 9.899999999999999 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content pass >>END_MODULE Read 788010 spots for SRR26075385.sra Written 788010 spots for SRR26075385.sra Read 788010 spots for SRR26075385.sra Written 788010 spots for SRR26075385.sra Read 788010 spots for SRR26075385.sra Written 788010 spots for SRR26075385.sra Read 788010 spots for SRR26075385.sra Written 788010 spots for SRR26075385.sra Read 788010 spots for SRR26075385.sra Written 788010 spots for SRR26075385.sra Read 788010 spots for SRR26075385.sra Written 788010 spots for SRR26075385.sra Read 788010 spots for SRR26075385.sra Written 788010 spots for SRR26075385.sra Read 788010 spots for SRR26075385.sra Written 788010 spots for SRR26075385.sra Read 788010 spots for SRR26075385.sra Written 788010 spots for SRR26075385.sra Read 788010 spots for SRR26075385.sra Written 788010 spots for SRR26075385.sra Read 788010 spots for SRR26075385.sra Written 788010 spots for SRR26075385.sra Read 788010 spots for SRR26075385.sra Written 788010 spots for SRR26075385.sra Read 788010 spots for SRR26075385.sra Written 788010 spots for SRR26075385.sra Read 788010 spots for SRR26075385.sra Written 788010 spots for SRR26075385.sra Read 788010 spots for SRR26075385.sra Written 788010 spots for SRR26075385.sra Read 788010 spots for SRR26075385.sra Written 788010 spots for SRR26075385.sra Read 788010 spots for SRR26075385.sra Written 788010 spots for SRR26075385.sra Read 788010 spots for SRR26075385.sra Written 788010 spots for SRR26075385.sra Read 788010 spots for SRR26075385.sra Written 788010 spots for SRR26075385.sra Read 788010 spots for SRR26075385.sra Written 788010 spots for SRR26075385.sra SRR ids: ['SRR26075385.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd__r1xmssz SRR26075385.sra spots: 15760200 blocks: [[1, 788010], [788011, 1576020], [1576021, 2364030], [2364031, 3152040], [3152041, 3940050], [3940051, 4728060], [4728061, 5516070], [5516071, 6304080], [6304081, 7092090], [7092091, 7880100], [7880101, 8668110], [8668111, 9456120], [9456121, 10244130], [10244131, 11032140], [11032141, 11820150], [11820151, 12608160], [12608161, 13396170], [13396171, 14184180], [14184181, 14972190], [14972191, 15760200]] SRR26075385 file size 5814041 SRR26075385 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o SRR26075385 SRR26075385_1.fastq SRR26075385_2.fastq Input file: SRR26075385_1.fastq Paired file: SRR26075385_2.fastq trimmed: SRR26075385-trimmed-pair1.fastq, SRR26075385-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Wed Feb 12 00:43:28 2025 >> started Wed Feb 12 00:43:46 2025 >> done (18.133s) 15760200 read pairs processed; of these: 45 ( 0.00%) short read pairs filtered out after trimming by size control 11463 ( 0.07%) empty read pairs filtered out after trimming by size control 15748692 (99.93%) read pairs available; of these: 2278273 (14.47%) trimmed read pairs available after processing 13470419 (85.53%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 18 1 0.00% 19 4 0.00% 20 4 0.00% 21 4 0.00% 22 4 0.00% 23 7 0.00% 24 9 0.00% 25 7 0.00% 26 11 0.00% 27 11 0.00% 28 5 0.00% 29 12 0.00% 30 6 0.00% 31 10 0.00% 32 7 0.00% 33 12 0.00% 34 23 0.00% 35 12 0.00% 36 13 0.00% 37 18 0.00% 38 15 0.00% 39 24 0.00% 40 18 0.00% 41 10 0.00% 42 25 0.00% 43 32 0.00% 44 26 0.00% 45 47 0.00% 46 24 0.00% 47 44 0.00% 48 45 0.00% 49 54 0.00% 50 63 0.00% 51 42 0.00% 52 78 0.00% 53 59 0.00% 54 85 0.00% 55 84 0.00% 56 100 0.00% 57 141 0.00% 58 147 0.00% 59 149 0.00% 60 204 0.00% 61 249 0.00% 62 302 0.00% 63 272 0.00% 64 355 0.00% 65 379 0.00% 66 401 0.00% 67 468 0.00% 68 556 0.00% 69 631 0.00% 70 728 0.00% 71 710 0.00% 72 940 0.01% 73 1124 0.01% 74 1273 0.01% 75 1570 0.01% 76 1649 0.01% 77 1704 0.01% 78 1998 0.01% 79 2202 0.01% 80 2514 0.02% 81 2933 0.02% 82 3161 0.02% 83 3726 0.02% 84 4181 0.03% 85 4569 0.03% 86 5284 0.03% 87 5545 0.04% 88 5873 0.04% 89 6470 0.04% 90 7237 0.05% 91 7974 0.05% 92 8538 0.05% 93 9243 0.06% 94 10493 0.07% 95 11204 0.07% 96 12063 0.08% 97 12972 0.08% 98 13442 0.09% 99 14191 0.09% 100 15102 0.10% 101 15847 0.10% 102 16800 0.11% 103 18013 0.11% 104 19549 0.12% 105 20218 0.13% 106 21574 0.14% 107 22684 0.14% 108 23420 0.15% 109 24119 0.15% 110 24954 0.16% 111 25959 0.16% 112 27220 0.17% 113 27826 0.18% 114 28522 0.18% 115 31090 0.20% 116 31931 0.20% 117 32729 0.21% 118 34436 0.22% 119 35164 0.22% 120 36368 0.23% 121 37575 0.24% 122 37304 0.24% 123 38607 0.25% 124 40387 0.26% 125 40971 0.26% 126 42433 0.27% 127 44366 0.28% 128 45471 0.29% 129 46249 0.29% 130 48089 0.31% 131 49441 0.31% 132 48373 0.31% 133 49128 0.31% 134 50723 0.32% 135 51701 0.33% 136 53850 0.34% 137 54655 0.35% 138 55704 0.35% 139 57412 0.36% 140 57263 0.36% 141 57863 0.37% 142 59572 0.38% 143 59653 0.38% 144 60757 0.39% 145 62131 0.39% 146 62391 0.40% 147 63683 0.40% 148 64817 0.41% 149 65925 0.42% 150 67434 0.43% 151 13470419 85.53% 15748692 reads passed initial QC criterion=sequence-density sequence-density=0.25 sequence-density-rank=1 fanout-score=4.18 fanout-score-rank=26 prefix-density=0.30 prefix-fanout=3.5 sequence=CCACATTTGCAGCCACTGCC criterion=fanout-score sequence-density=0.01 sequence-density-rank=39 fanout-score=167.81 fanout-score-rank=1 prefix-density=0.08 prefix-fanout=11.3 sequence=CTTTAGCTTCTACTTTTATTTAATAGTTTTATAGATTACACAAAGGAAATACAACACAAGATCTCCCCACAAATCACACACATTGATGCAGTACTGAACTCGTTGCACGAAAGCGCTTAGATATATATTATACAAGTACTAGCATGATCACAAACATGTGATGCTTATTGGTCGAGATCGATGACCCCTTCTATTACTCCGTGCTAAGGGCTTCGTCGATGTCTTTAGTCATATGAACCATAAGATCAACATAAATCTCTGGAACCGGGACTTCAGGATGGAGTTTTTCGTATTCAATGGTCAGTTTTGCCAAGCAGCCCGAGCCTTTTGGTGTAAGCTGCCAGACGGGCCTATAGACCTTGTAAATTTTCATGACATCTCCTTCCAAACCATTAAGAGTTATGATCTTGTTCTCATCATCGAAGGAAACCTCCTCTTTAAAGACCCCGGCTTTCCCTCCGATTGTGTACTGCCAAATCCTGATAGAGCCCGCAGTCTCCCAGTCACCTGC criterion=sequence-density sequence-density=0.38 sequence-density-rank=1 fanout-score=2.52 fanout-score-rank=33 prefix-density=0.39 prefix-fanout=2.5 sequence=ATGTACCCTGACTTAGGTTTCTCAGA criterion=fanout-score sequence-density=0.01 sequence-density-rank=34 fanout-score=170.03 fanout-score-rank=1 prefix-density=0.16 prefix-fanout=9.5 sequence=AGAGAAAAGATAAGCTAGGCAAGATGGTTTTACTAGACAAGATGTGGGATGATGTTGTTGCTGGACCTCAGCCAGAACGTGGCCTTGGCAAGCTTAGAAAGATCAGCACCAGACCACTTAACATCAAAGATATTGACGTCGGAGAGGGGAGCAGTCCTGTTAATAAGTTTCAGAGGTCCATGACTATGCCAGGAACTCCAGGGACACCGACGACACCAGTGACCCCTACAACCCCAGTGTCGGCGCGTAGCAATGTTTGGAGGAGCGTGTTCCACCCTGGTAGCAACCTTGCTACTAAGAATATTGGTGCTCATGTTTTTGACAAGCCACAGCCTAACACACCCACTGTCTATGACTGGATGTACAGTGGAGAGACGAAGAGCGAGCATCGTTGATGAGGTTGCCTTCAACCAAGGTTGCCCATGTAAATACGTACTGTGTTTTGTTTTTCAGTACTCATCTGCAATATGTCTCTTGTTGTTATGGTTCTACGGTTCTACCGTGCCTGGAA SRR26075385 testing PE reads STAR mapping to Ensembl genome Started job on | Feb 12 00:44:27 Started mapping on | Feb 12 00:44:27 Finished on | Feb 12 00:47:03 Mapping speed, Million of reads per hour | 363.43 Number of input reads | 15748692 Average input read length | 295 UNIQUE READS: Uniquely mapped reads number | 14367859 Uniquely mapped reads % | 91.23% Average mapped length | 293.78 Number of splices: Total | 13606070 Number of splices: Annotated (sjdb) | 13286068 Number of splices: GT/AG | 13362604 Number of splices: GC/AG | 186867 Number of splices: AT/AC | 12614 Number of splices: Non-canonical | 43985 Mismatch rate per base, % | 0.38% Deletion rate per base | 0.03% Deletion average length | 3.25 Insertion rate per base | 0.02% Insertion average length | 2.60 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 388186 % of reads mapped to multiple loci | 2.46% Number of reads mapped to too many loci | 58398 % of reads mapped to too many loci | 0.37% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 5.73% % of reads unmapped: other | 0.20% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 992647 992647 992647 N_multimapping 388186 388186 388186 N_noFeature 402555 14201651 476163 N_ambiguous 169732 790 76747 UnstrandedReadsAssigned:13795572 PositiveStrandReadsAssigned:165418 NegativeStrandReadsAssigned:13814949 Dataset is classified negative stranded MeadianReadLen=151 20thPercentileLength=151 echo kmer=147 SRR26075385 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,400 [index] number of k-mers: 62,057,036 [index] number of equivalence classes: 130,681 [quant] running in paired-end mode [quant] will process pair 1: SRR26075385-trimmed-pair1.fastq SRR26075385-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 15,748,692 reads, 13,941,856 reads pseudoaligned [quant] estimated average fragment length: 219.306 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,050 rounds 52401 SRR26075385.ke.tsv 34699 SRR26075385.se.tsv 87100 total ==> SRR26075385.ke.tsv <== target_id length eff_length est_counts tpm Potri.005G200100.1.v4.1 2018 1799.69 1990 70.3369 Potri.005G024800.1.v4.1 1035 816.694 429 33.4139 Potri.004G059700.1.v4.1 961 742.699 7 0.599534 Potri.007G009000.2.v4.1 1416 1197.69 0 0 Potri.003G141000.2.v4.1 2943 2724.69 596 13.9142 Potri.016G087400.1.v4.1 270 87.6314 1650.26 1197.9 Potri.015G069301.1.v4.1 564 348.221 0 0 Potri.010G195200.1.v4.1 1773 1554.69 585.777 23.9671 Potri.012G127500.1.v4.1 977 758.699 14151 1186.44 ==> SRR26075385.se.tsv <== Potri.001G166300.v4.1 0 Potri.001G448400.v4.1 171 Potri.001G233950.v4.1 0 Potri.001G122700.v4.1 393 Potri.001G212900.v4.1 0 Potri.001G182400.v4.1 0 Potri.001G256600.v4.1 0 Potri.001G040500.v4.1 2 Potri.001G416900.v4.1 0 Potri.001G452600.v4.1 916 SRR26075385 completed mapping pipeline successfully