Starting /dee2/code/volunteer_pipeline.sh SRR26075386 current disk space = 3051207544832 free memory = 1570947836 SRR26075386 SRAfilesize b9c0f265835fef35660e6dc5669c766f SRR26075386.sra SRR26075386.sra file validated SRR26075386 is paired end SRR26075386 is conventional basespace SRR26075386 read1 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR26075386_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.681 37.0 37.0 37.0 37.0 37.0 2 36.6125 37.0 37.0 37.0 37.0 37.0 3 36.624 37.0 37.0 37.0 37.0 37.0 4 36.6335 37.0 37.0 37.0 37.0 37.0 5 36.7325 37.0 37.0 37.0 37.0 37.0 6 36.7345 37.0 37.0 37.0 37.0 37.0 7 36.6645 37.0 37.0 37.0 37.0 37.0 8 36.6385 37.0 37.0 37.0 37.0 37.0 9 36.6175 37.0 37.0 37.0 37.0 37.0 10-14 36.65745 37.0 37.0 37.0 37.0 37.0 15-19 36.6457 37.0 37.0 37.0 37.0 37.0 20-24 36.54800000000001 37.0 37.0 37.0 37.0 37.0 25-29 36.503499999999995 37.0 37.0 37.0 37.0 37.0 30-34 36.394999999999996 37.0 37.0 37.0 37.0 37.0 35-39 36.43130000000001 37.0 37.0 37.0 37.0 37.0 40-44 36.364 37.0 37.0 37.0 37.0 37.0 45-49 36.331 37.0 37.0 37.0 37.0 37.0 50-54 36.374399999999994 37.0 37.0 37.0 37.0 37.0 55-59 36.3043 37.0 37.0 37.0 37.0 37.0 60-64 36.240700000000004 37.0 37.0 37.0 37.0 37.0 65-69 36.1851 37.0 37.0 37.0 37.0 37.0 70-74 36.1703 37.0 37.0 37.0 37.0 37.0 75-79 36.146699999999996 37.0 37.0 37.0 37.0 37.0 80-84 36.0779 37.0 37.0 37.0 37.0 37.0 85-89 36.0723 37.0 37.0 37.0 37.0 37.0 90-94 36.02139999999999 37.0 37.0 37.0 37.0 37.0 95-99 36.0469 37.0 37.0 37.0 37.0 37.0 100-104 35.9427 37.0 37.0 37.0 37.0 37.0 105-109 35.840799999999994 37.0 37.0 37.0 37.0 37.0 110-114 35.75920000000001 37.0 37.0 37.0 37.0 37.0 115-119 35.6442 37.0 37.0 37.0 37.0 37.0 120-124 35.642399999999995 37.0 37.0 37.0 37.0 37.0 125-129 35.563900000000004 37.0 37.0 37.0 37.0 37.0 130-134 35.3433 37.0 37.0 37.0 37.0 37.0 135-139 35.3599 37.0 37.0 37.0 32.2 37.0 140-144 35.17 37.0 37.0 37.0 27.4 37.0 145-149 35.187599999999996 37.0 37.0 37.0 25.0 37.0 150-151 34.972 37.0 37.0 37.0 31.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.0 1101 2 0.0 1101 3 0.0 1101 4 0.0 1101 5 0.0 1101 6 0.0 1101 7 0.0 1101 8 0.0 1101 9 0.0 1101 10-14 0.0 1101 15-19 0.0 1101 20-24 0.0 1101 25-29 0.0 1101 30-34 0.0 1101 35-39 0.0 1101 40-44 0.0 1101 45-49 0.0 1101 50-54 0.0 1101 55-59 0.0 1101 60-64 0.0 1101 65-69 0.0 1101 70-74 0.0 1101 75-79 0.0 1101 80-84 0.0 1101 85-89 0.0 1101 90-94 0.0 1101 95-99 0.0 1101 100-104 0.0 1101 105-109 0.0 1101 110-114 0.0 1101 115-119 0.0 1101 120-124 0.0 1101 125-129 0.0 1101 130-134 0.0 1101 135-139 0.0 1101 140-144 0.0 1101 145-149 0.0 1101 150-151 0.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 22 2.0 23 5.0 24 2.0 25 6.0 26 4.0 27 9.0 28 16.0 29 27.0 30 41.0 31 30.0 32 60.0 33 81.0 34 152.0 35 454.0 36 2902.0 37 209.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 45.666332665330664 12.875751503006011 6.88877755511022 34.569138276553105 2 20.200000000000003 13.525 32.550000000000004 33.725 3 18.575 17.7 27.474999999999998 36.25 4 21.224999999999998 24.825 25.474999999999998 28.475 5 24.224999999999998 28.4 25.474999999999998 21.9 6 22.575 31.75 22.45 23.225 7 15.475 27.775 38.175 18.575 8 17.175 28.599999999999998 30.25 23.974999999999998 9 18.15 22.0 37.05 22.8 10-14 19.45597279863993 29.746487324366218 27.586379318965946 23.211160558027903 15-19 19.695 27.950000000000003 27.92 24.435000000000002 20-24 20.285 29.28 26.935 23.5 25-29 19.56 28.18 27.744999999999997 24.515 30-34 19.34 27.515 28.49 24.654999999999998 35-39 20.61 27.83 27.675 23.885 40-44 20.315 29.220000000000002 26.695 23.77 45-49 21.07 26.834999999999997 27.63 24.465 50-54 20.84 27.145000000000003 28.09 23.925 55-59 19.675 27.694999999999997 27.644999999999996 24.985 60-64 20.115 28.53 27.084999999999997 24.27 65-69 20.39 27.650000000000002 27.544999999999998 24.415 70-74 19.925 27.96 26.97 25.145 75-79 20.54 27.22 28.255000000000003 23.985 80-84 21.015 27.705000000000002 28.28 23.0 85-89 20.16 27.994999999999997 27.445000000000004 24.4 90-94 20.305 27.805000000000003 27.694999999999997 24.195 95-99 19.27 27.83 27.939999999999998 24.959999999999997 100-104 20.355 27.47 28.225 23.95 105-109 20.745 26.895000000000003 28.265 24.095 110-114 20.965 27.145000000000003 27.639999999999997 24.25 115-119 20.119999999999997 27.334999999999997 28.199999999999996 24.345 120-124 21.23 27.735 26.935 24.099999999999998 125-129 21.22 27.365000000000002 28.134999999999998 23.28 130-134 21.315 27.310000000000002 27.36 24.015 135-139 21.92 27.395000000000003 26.87 23.815 140-144 21.310000000000002 27.305 26.840000000000003 24.545 145-149 22.470000000000002 28.9 25.1 23.53 150-151 21.825 28.212500000000002 26.2625 23.7 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.5 6 0.5 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.5 15 0.5 16 0.0 17 0.0 18 0.0 19 0.0 20 1.0 21 1.0 22 0.5 23 0.5 24 0.0 25 2.0 26 5.0 27 8.5 28 10.0 29 8.5 30 14.5 31 20.0 32 22.0 33 25.0 34 35.5 35 53.5 36 66.5 37 92.5 38 117.0 39 148.5 40 181.0 41 206.5 42 232.0 43 253.5 44 277.5 45 266.5 46 282.5 47 295.5 48 262.5 49 216.0 50 163.0 51 135.0 52 127.5 53 104.5 54 77.5 55 58.5 56 47.0 57 40.5 58 39.5 59 34.5 60 15.0 61 12.0 62 12.0 63 7.5 64 3.0 65 3.0 66 3.0 67 3.0 68 2.5 69 1.5 70 1.0 71 0.5 72 0.5 73 0.5 74 0.0 75 0.0 76 0.0 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.005 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150-151 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 57.275 #Duplication Level Percentage of deduplicated Percentage of total 1 59.75556525534701 34.225 2 22.47926669576604 25.75 3 9.384548232213008 16.125 4 4.146660846791795 9.5 5 2.4879965080750766 7.124999999999999 6 0.7420340462680053 2.55 7 0.4364906154517678 1.7500000000000002 8 0.30554343081623747 1.4000000000000001 9 0.08729812309035355 0.44999999999999996 >10 0.1745962461807071 1.125 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CCTCAACCGAATCTAAATCAGGCAAAGCTCCACCACAGGCAAAGTAGAGC 13 0.325 No Hit GGCATGGTTGAGACTGTGTCAGGTCCAATGAGGGGAGCAACATATAAAGT 12 0.3 No Hit GCAACCCAAAAGACAGGGTGTTTCTTCCCTGATTTGAAAGTGAAACTGGC 10 0.25 No Hit CGTTAGTGTTGGGTTAGAGCTTTGTACATGGTTTCATGGTACACTGTCAC 10 0.25 No Hit CTCAGCGCTGTTCTTCAAAATCCACTTTTCAACAGTTCCACAGTCTACTG 9 0.22499999999999998 No Hit GCTCAATATCATTGCCATCTAGGACGAGCTCATCCTTCACCTTTTCAGAT 9 0.22499999999999998 No Hit GTGGGGATCACCAGATGATCAATGCCATAAGCATGGTATAAAGAAGTTGG 8 0.2 No Hit GCCGTTTCTTGCCTCTAAAGCAGATGTATCAGTCTGTGGCTTACTCGATT 8 0.2 No Hit TCCTTGTCAGCAAATGAATTGAGATAGAAGATGGCATTCTGGAATGCCTT 8 0.2 No Hit GTCGACTTAATGCTTTGCATCTTCTTCTTCACAGAACTTAGCATAGTCAT 8 0.2 No Hit AGATCATGTTGAAATGGCCAAAAAGCCTCCTTTAAGTTTCTCAAAATCTT 8 0.2 No Hit CACTGAATGAAGCATTGAAACAACCATGGTAATCACCAGGAGGTAAGGAT 8 0.2 No Hit CCCAATTGCAATAGCATCTAAATAGTAAGGCACGACAACACCTCCAACAC 8 0.2 No Hit GGGAGGTTTTTATTTCTCAAGACTGTCTGATTCTATACAGCTGTGAGTGA 7 0.17500000000000002 No Hit GCACCCACAGTTTTGTCCACTAATTTTCCATCTTTCAGGAAAATAAAAGT 7 0.17500000000000002 No Hit GCCACGCCGTAAACTTTCCATGGGCCACCAGGTGTGTCGGAGAGTGGGAG 7 0.17500000000000002 No Hit GAGGTGTGGATGCTGCTGGACCACAATCTTCACTCTTTCCAGGGATTGAA 7 0.17500000000000002 No Hit GCCAAAGTTATTAAATGCTCTAGCACAGTCCACAAGCTGCTGTTCAGACA 7 0.17500000000000002 No Hit GGGCTGAGGGGAAGATCGGTGCTGTGGGTAGAGGGCGCCGATGTTGAGGT 7 0.17500000000000002 No Hit GTGGGGTTCATGCCGCCAAAAATGGAGGCGAAGTCGTCGAGTAAGAGGGC 7 0.17500000000000002 No Hit CTATCATCCTTATCTAGCAGGGTAGAGAGATAAGAAATGGACTCTTGCCA 7 0.17500000000000002 No Hit CACCATCTTGCTCATATTTCCTGACCCTTTTAGCACTTCTTGAGTTGCAA 7 0.17500000000000002 No Hit CTTCGCATTAGGAATGTACTCGATAGGGTAGGATGCTCGAGTGTTCTCTG 7 0.17500000000000002 No Hit GTGACTGCTGTTAAACTTTGAATAATGGGAACTGATAAGAGAACTTCTCC 6 0.15 No Hit GGCCAGGTTTTCTTGGTGGAGAAGACTAGCTTTCTTCCTCAGCTTCTCGT 6 0.15 No Hit CCACTGTAAGAAACAAGACTACTGGTGCTTTTACCATCTGCGCTGTAGGA 6 0.15 No Hit ATAAACTTGTGATTTGTCTGCGATCCATCTGGCTAACGCATCGACAGGCA 6 0.15 No Hit CCCCGCGAAGCAGCAACACTTGAAAAACTGAATCGAGTCCCACGCCTAAA 6 0.15 No Hit TGCCTATAGAATTAAAGAAAGCGATTATCCTCTAAGTGGAAATAAAGTGT 6 0.15 No Hit CGTCTTTCATGTATCCCTTCTTAACACAAGACAGTTTACTGGCGGAAGCG 6 0.15 No Hit GCTGCAAATAACTGGCAAGAAAAGCATAAGAGAGGAATCATAGAATAGAG 6 0.15 No Hit TTCCTGGATAGTGTTCTCTGTTTGAATGCTTCCATTGTTGAATAGACACC 6 0.15 No Hit CACAACAAGATTGCCTAGCATTCCATCTATAGCAGACTAACAATAAACAT 6 0.15 No Hit CTCGTCCTGTAATTGACTGGTTTCTTTTCATCGGCGAGTTTAAAAGTGAA 6 0.15 No Hit GCCCTCTTCCTCAGCTCATCCATGATTGGGTTCTCTGGATTTATCTCCAT 6 0.15 No Hit GCGATGGATCGAGGTCATGACTGGACACTGCATCGGAAGACACTTCGTGC 6 0.15 No Hit GGGAGACCTGACAAAAGCTGGAGGTGCAGGAAAAGATGACACATATGCAT 6 0.15 No Hit TATTAGGAGACCTAAACCATAAGCAGTCATTGCCCACAAGAAGTATCCGG 6 0.15 No Hit CCATTCCTTTGCAAACATCAATCGCAAACTTCAGCAATTGAGGGAGCTTC 6 0.15 No Hit GTAGATTCAAAGGCATGAGTGTATCCGGTTTAGCTCCGCAGACTCCATGC 6 0.15 No Hit CTTAGAGACAGAGTCCTAACTCCATTATCATGAATATAAATGCCTACGTC 5 0.125 No Hit TTACCAACAAGCCTATCAATCCACATCCAATCAAAACTTGCAAACTCACG 5 0.125 No Hit CTCACTCTTAAATATATGCATTCGATTTCTGAAGTTACTGAAGATGAACC 5 0.125 No Hit GAGCAAGTTTGCAAGTTCACCGAGCTCCATATCCTTGGTGTTCATCTCCA 5 0.125 No Hit GGTTGAGGTGCCAGCAAGCATCTGCTCGGGGGTAGAAAAGTGACGCTGAC 5 0.125 No Hit CTCCAATTCAGCCATATGCAACTTCCTCCACTTCTCCTCCATCTCTGCCT 5 0.125 No Hit CCTATGTCGAGACTTGCTAGCAGTCGCCCCATTTTCCTCTTTGTCCAAAG 5 0.125 No Hit GCTGCATGCAATGCTCTTCAGCATCAGCCTGAGTGCAAATAATCACCACT 5 0.125 No Hit CATCGATCTTGGGAATGACAATGTTAAGCACCCCGTCATTCATAACAGCA 5 0.125 No Hit AGGAGGGCGTCCTTGGCATAATCATTCTCTGCTCTTGCTTTGATGATTTG 5 0.125 No Hit GGATAATCGATATGCAGGGATGCAAGACGGTCAAGAGCTGAGGGAGGCAT 5 0.125 No Hit TACCTGATAGCTTCCAACTAGATGAATCACAATACAAGCATTAGCAATGT 5 0.125 No Hit CTCATTTTCGCTAAGTTGAACCTGCGGTTGTTTGGCTAACCTTGTTTGCC 5 0.125 No Hit CACTTCTCAAATGAACCATAAATTGAATCAAGCTTCTCAATAGCTGATTT 5 0.125 No Hit AGTGTATGATACTTCACCCCTCATAGACGAACTAAACACCAGTTTGAGAT 5 0.125 No Hit CCTTTGGATGATAGCTGTTCATAGACTTCTACCAACAACGGAGTGAAATT 5 0.125 No Hit GTGCAGACCTCAGAGGAGAAGGGGTAGGACTCGAAAAAGGTGCAGTGTAC 5 0.125 No Hit CTCACATTCATCAAGCTCCATGTCAGTAAGCTCAGCTGAGGTTCGTGGAG 5 0.125 No Hit GCATTAATTACCAACCTCCCAAACCCCAAATTCAAAAACCAACAAACGGT 5 0.125 No Hit CTTTTTTCACAGGGGGCAAAAATTACCCAACATTCAGCAAAGCAAGCAGA 5 0.125 No Hit GCAAAAGTTTCGGCTTATGTAGGTTCCCTGATCACTACCAAAATGTAATC 5 0.125 No Hit CCACCAACAAGGACAGCATCATGGACAGTGCTCTTGTCCATCTTCGCATC 5 0.125 No Hit CTGCTAAACAACTTGGTGAAAGACAGCCCCATTTTCTCACGTCTCGGCAG 5 0.125 No Hit GCAACAATAAGTGAATTTCCAGGTGCAGCTATAAATGCCTGACGGATCTT 5 0.125 No Hit GGTAATTTCAAGATCGCTACCAACATCCAACCCTAGGAGCTGCCCAGTCA 5 0.125 No Hit GCCTTTTCCTTGTCAGTGAAAACTCCAGTTGACTCCACAATATACTCAGC 5 0.125 No Hit GCCTCTAATCATTTTCAGAGTTTGATGATTTAAGGAAGATAAAATCGATC 5 0.125 No Hit CAGCTGCCGAATCAGCACCGGAAGGTGACCAATTCCCAGTGATGACACTG 5 0.125 No Hit GATCAGGTGCGGAATTGGACGAGGAAACTATACAGGAAGAACCGCCTAAA 5 0.125 No Hit GATCACTATCTGACCAACCCTCAACCCTAGCATCAGGATCAGACCAAAGT 5 0.125 No Hit TGTCACTGCAATTAAGGTTTCGAACATGGATTAGTGGTGGCTTCAACCAG 5 0.125 No Hit CCTGAAATCACCTCAACCGAATTGCTCCTGTCTCCCTCTATCTTCTGCAA 5 0.125 No Hit ACCGCCCACTCATTTTCTGCAGCAATCCTTCCTGAGCCACGGCCACCCAT 5 0.125 No Hit ACCATATCTATAATTATCACCTTCCCAGCTTTCTGCTGTCCCGTACTCAT 5 0.125 No Hit GTCATCTGTCTCTGGTCTCCAGTTACTCGTAGGCCTTCACCACCATTTAC 5 0.125 No Hit GGCCATCCTCGAGCTGCTTTCCGGCAAAGATCAACCTTTGCTGGTCCGGA 5 0.125 No Hit CCTTGACTTGCTATTGCGGATACCATTTGAGAAGCAATATTGAATCTTTG 5 0.125 No Hit AACACGAACAACATCATAAAAACTACACATATCATGCAGTGCTTTATCTT 5 0.125 No Hit GGAAGCTATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTG 5 0.125 No Hit GGTTGGCTCGAACCGTTCTGGTCCGGGATAGCTTGGATTAAGCAAGCAGC 5 0.125 No Hit TCCCACTGTTCTCAACCAAACCAACTGGAATAGCAGAATCCTCAAAGTCT 5 0.125 No Hit CCAAAAAGAACAATCTAGAACTCTCAAATCCAAAACGAACACCTTCTTCT 5 0.125 No Hit GGGGATGATGGGGTTGGTGTTGTGGAGATGGGTTTTGATCAGGACCGTTG 5 0.125 No Hit GTCTCCAATCTCTGCAAAGAGCTCCCTTATGTCTTCATTGGTCACGCCAT 5 0.125 No Hit CTCTTCATCAACCTCTCCCTCGTTAGAATCAACAGGCATTCTTGCCTTCA 5 0.125 No Hit TTTTGAATTGACGCACCGGTAAAGTTGGGCTGGTGAAAATCGTCATGGTG 5 0.125 No Hit GCCAATACCAACTTTCTCCCCAACTTTCCCCACGAGCTTGTGGAAGAAGC 5 0.125 No Hit CCTCTGCTCGATCTTTTCTCCTCTTTGACGAAGAGGAGGAATATTCCTCT 5 0.125 No Hit CTCAGCAGATTCAGAAGTCTCAATAACAGCAACTGCTTCTGACTCCTCCA 5 0.125 No Hit GCCATTGCTATATAGTAAAAGTGACAAAATAACTGCTTAAGTTCCACAAA 5 0.125 No Hit CCCTAAATCAGCATACCATCCCCTAGTTGGCCCATTTATACCATCCGATT 5 0.125 No Hit GCAGATTGGCTCCCAGGGGAATCATGTGAACTGTGTGACTGCGGATGGTC 5 0.125 No Hit GGCAGGAAAATGCAATTTAAAAGAGCAAGTACCGAAGATTATAGCATGAG 5 0.125 No Hit ATGAACTTTGCCAATCAGATCCAGCCGCACAGCATCGTCAGATGGTGAGA 5 0.125 No Hit GTGGTGTAACTTCATCCTGGTAATAGATCCTTAATCCGATCTCACCTTGT 5 0.125 No Hit GTCAACACAAGCCTTAGTTAAGGCCTCTGTTTCTAATCCCATAGCCTCTG 5 0.125 No Hit CCACTTTCTATTACGGTAACAATATCTTACAAAACCAAACAAATACATTC 5 0.125 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.025 0.0 0.0 0.0 0.0 50-51 0.025 0.0 0.0 0.0 0.0 52-53 0.025 0.0 0.0 0.0 0.0 54-55 0.025 0.0 0.0 0.0 0.0 56-57 0.025 0.0 0.0 0.0 0.0 58-59 0.025 0.0 0.0 0.0 0.0 60-61 0.025 0.0 0.0 0.0 0.0 62-63 0.025 0.0 0.0 0.0 0.0 64-65 0.025 0.0 0.0 0.0 0.0 66-67 0.025 0.0 0.0 0.0 0.0 68-69 0.025 0.0 0.0 0.0 0.0 70-71 0.025 0.0 0.0 0.0 0.0 72-73 0.05 0.0 0.0 0.0 0.0 74-75 0.1 0.0 0.0 0.0 0.0 76-77 0.15 0.0 0.0 0.0 0.0 78-79 0.175 0.0 0.0 0.0 0.0 80-81 0.2 0.0 0.0 0.0 0.0 82-83 0.21250000000000002 0.0 0.0 0.0 0.0 84-85 0.25 0.0 0.0 0.0 0.0 86-87 0.3125 0.0 0.0 0.0 0.0 88-89 0.4625 0.0 0.0 0.0 0.0 90-91 0.4875 0.0 0.0 0.0 0.0 92-93 0.5875 0.0 0.0 0.0 0.0 94-95 0.725 0.0 0.0 0.0 0.0 96-97 0.925 0.0 0.0 0.0 0.0 98-99 1.2000000000000002 0.0 0.0 0.0 0.0 100-101 1.45 0.0 0.0 0.0 0.0 102-103 1.6 0.0 0.0 0.0 0.0 104-105 1.7875 0.0 0.0 0.0 0.0 106-107 1.9375 0.0 0.0 0.0 0.0 108-109 2.1375 0.0 0.0 0.0 0.0 110-111 2.275 0.0 0.0 0.0 0.0 112-113 2.5 0.0 0.0 0.0 0.0 114-115 2.575 0.0 0.0 0.0 0.0 116-117 3.0125 0.0 0.0 0.0 0.0 118-119 3.475 0.0 0.0 0.0 0.0 120-121 3.6624999999999996 0.0 0.0 0.0 0.0 122-123 4.0375 0.0 0.0 0.0 0.0 124-125 4.575 0.0 0.0 0.0 0.0 126-127 5.137499999999999 0.0 0.0 0.0 0.0 128-129 5.725 0.0 0.0 0.0 0.0 130-131 6.1875 0.0 0.0 0.0 0.0 132-133 7.0 0.0 0.0 0.0 0.0 134-135 7.7875 0.0 0.0 0.0 0.0 136-137 8.5 0.0 0.0 0.0 0.0 138-139 9.25 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCAGCAT 10 0.006830828 145.0 2 TCTGTAA 10 0.006830828 145.0 5 AAGCATG 10 0.006830828 145.0 9 >>END_MODULE SRR26075386 read2 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR26075386_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.0345 37.0 37.0 37.0 37.0 37.0 2 36.1515 37.0 37.0 37.0 37.0 37.0 3 36.256 37.0 37.0 37.0 37.0 37.0 4 36.1775 37.0 37.0 37.0 37.0 37.0 5 36.2495 37.0 37.0 37.0 37.0 37.0 6 36.225 37.0 37.0 37.0 37.0 37.0 7 36.1645 37.0 37.0 37.0 37.0 37.0 8 36.294 37.0 37.0 37.0 37.0 37.0 9 36.2525 37.0 37.0 37.0 37.0 37.0 10-14 36.2211 37.0 37.0 37.0 37.0 37.0 15-19 36.2028 37.0 37.0 37.0 37.0 37.0 20-24 36.129400000000004 37.0 37.0 37.0 37.0 37.0 25-29 36.062200000000004 37.0 37.0 37.0 37.0 37.0 30-34 36.011199999999995 37.0 37.0 37.0 37.0 37.0 35-39 35.936800000000005 37.0 37.0 37.0 37.0 37.0 40-44 35.9027 37.0 37.0 37.0 37.0 37.0 45-49 35.8593 37.0 37.0 37.0 37.0 37.0 50-54 35.7915 37.0 37.0 37.0 37.0 37.0 55-59 35.76090000000001 37.0 37.0 37.0 37.0 37.0 60-64 35.724000000000004 37.0 37.0 37.0 37.0 37.0 65-69 35.708600000000004 37.0 37.0 37.0 37.0 37.0 70-74 35.6738 37.0 37.0 37.0 37.0 37.0 75-79 35.5969 37.0 37.0 37.0 37.0 37.0 80-84 35.5647 37.0 37.0 37.0 37.0 37.0 85-89 35.536 37.0 37.0 37.0 37.0 37.0 90-94 35.3941 37.0 37.0 37.0 37.0 37.0 95-99 35.5407 37.0 37.0 37.0 37.0 37.0 100-104 35.3766 37.0 37.0 37.0 34.6 37.0 105-109 35.3759 37.0 37.0 37.0 34.6 37.0 110-114 35.204699999999995 37.0 37.0 37.0 32.2 37.0 115-119 35.3307 37.0 37.0 37.0 37.0 37.0 120-124 35.1038 37.0 37.0 37.0 27.4 37.0 125-129 35.1686 37.0 37.0 37.0 29.8 37.0 130-134 35.1241 37.0 37.0 37.0 27.4 37.0 135-139 34.98479999999999 37.0 37.0 37.0 25.0 37.0 140-144 34.984899999999996 37.0 37.0 37.0 25.0 37.0 145-149 34.9428 37.0 37.0 37.0 25.0 37.0 150-151 34.53375 37.0 37.0 37.0 25.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.0 1101 2 0.0 1101 3 0.0 1101 4 0.0 1101 5 0.0 1101 6 0.0 1101 7 0.0 1101 8 0.0 1101 9 0.0 1101 10-14 0.0 1101 15-19 0.0 1101 20-24 0.0 1101 25-29 0.0 1101 30-34 0.0 1101 35-39 0.0 1101 40-44 0.0 1101 45-49 0.0 1101 50-54 0.0 1101 55-59 0.0 1101 60-64 0.0 1101 65-69 0.0 1101 70-74 0.0 1101 75-79 0.0 1101 80-84 0.0 1101 85-89 0.0 1101 90-94 0.0 1101 95-99 0.0 1101 100-104 0.0 1101 105-109 0.0 1101 110-114 0.0 1101 115-119 0.0 1101 120-124 0.0 1101 125-129 0.0 1101 130-134 0.0 1101 135-139 0.0 1101 140-144 0.0 1101 145-149 0.0 1101 150-151 0.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 12 1.0 13 2.0 14 2.0 15 3.0 16 3.0 17 4.0 18 8.0 19 6.0 20 8.0 21 1.0 22 10.0 23 7.0 24 7.0 25 13.0 26 12.0 27 18.0 28 19.0 29 24.0 30 34.0 31 34.0 32 56.0 33 96.0 34 231.0 35 758.0 36 2485.0 37 158.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.2 23.799999999999997 10.925 21.075 2 29.9 27.500000000000004 27.275 15.325 3 20.974999999999998 27.125 32.95 18.95 4 24.775 33.2 23.674999999999997 18.35 5 26.8 33.650000000000006 22.25 17.299999999999997 6 20.175 39.725 21.05 19.05 7 22.1 23.275000000000002 36.199999999999996 18.425 8 21.475 27.3 27.3 23.925 9 23.599999999999998 24.5 28.95 22.95 10-14 24.93 28.475 25.564999999999998 21.029999999999998 15-19 24.09 28.535 27.41 19.965 20-24 23.875 27.525 26.995 21.605 25-29 24.675 27.62 26.229999999999997 21.475 30-34 23.625 28.315 26.845000000000002 21.215 35-39 24.11 28.37 26.284999999999997 21.235 40-44 23.87 28.42 27.025 20.685000000000002 45-49 24.295 28.54 27.215 19.950000000000003 50-54 23.674999999999997 28.389999999999997 27.384999999999998 20.549999999999997 55-59 24.64 27.650000000000002 26.99 20.72 60-64 23.655 28.395 27.37 20.580000000000002 65-69 24.19 28.485 26.56 20.765 70-74 24.635 28.825 26.029999999999998 20.51 75-79 23.625 28.065 26.740000000000002 21.57 80-84 23.82 29.445 26.534999999999997 20.200000000000003 85-89 25.205 27.815 26.525 20.455000000000002 90-94 24.36 28.88 26.575 20.185 95-99 24.485 29.659999999999997 26.075 19.78 100-104 25.795 28.505000000000003 25.97 19.73 105-109 24.195 28.65 26.235000000000003 20.919999999999998 110-114 24.59 28.67 27.21 19.53 115-119 25.295 28.275 27.439999999999998 18.990000000000002 120-124 24.85 28.794999999999998 26.775 19.580000000000002 125-129 25.155 27.46 27.46 19.925 130-134 25.19 28.26 26.724999999999998 19.825 135-139 25.155 27.839999999999996 26.99 20.015 140-144 26.605 27.334999999999997 26.950000000000003 19.11 145-149 25.945 28.935 25.535000000000004 19.585 150-151 27.450000000000003 26.8 25.874999999999996 19.875 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 1.0 9 1.5 10 1.5 11 1.0 12 0.0 13 0.5 14 1.5 15 2.5 16 2.0 17 1.5 18 1.0 19 1.0 20 1.5 21 0.5 22 1.0 23 2.0 24 1.5 25 5.0 26 9.5 27 6.0 28 5.0 29 8.0 30 7.5 31 15.0 32 18.0 33 20.0 34 39.5 35 45.5 36 52.0 37 75.5 38 103.5 39 134.0 40 186.0 41 230.5 42 234.5 43 267.0 44 306.0 45 290.0 46 281.5 47 293.0 48 260.5 49 213.5 50 175.5 51 138.0 52 124.0 53 103.5 54 75.0 55 59.0 56 35.0 57 27.5 58 23.0 59 15.5 60 17.0 61 10.0 62 10.0 63 9.0 64 5.5 65 4.5 66 4.5 67 6.0 68 3.5 69 1.0 70 0.5 71 0.5 72 1.0 73 1.5 74 0.5 75 0.0 76 0.5 77 0.5 78 0.5 79 0.5 80 0.0 81 0.5 82 1.5 83 1.5 84 0.5 85 0.5 86 1.0 87 1.0 88 0.5 89 1.0 90 1.0 91 0.0 92 0.5 93 0.5 94 0.0 95 0.5 96 0.5 97 0.0 98 0.0 99 0.5 100 4.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150-151 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 58.825 #Duplication Level Percentage of deduplicated Percentage of total 1 62.55843603909902 36.8 2 20.356991075223117 23.95 3 9.26476838079048 16.35 4 4.037399065023375 9.5 5 2.08244793880153 6.125 6 0.8499787505312366 3.0 7 0.3399915002124947 1.4000000000000001 8 0.254993625159371 1.2 9 0.08499787505312367 0.44999999999999996 >10 0.16999575010624735 1.225 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG 14 0.35000000000000003 No Hit GACAGGGATCGTCCTGCGATGAATCTCCATTTTTCATCTACCTATGCTCT 13 0.325 No Hit CCTTGATCTACGAGGAAAGGCTGCTGTGGCTCAAGCAGGCCTAGCATACA 12 0.3 No Hit GCATGATATAAAAGATGCTGGATGTGGCCTCGACGCCTCATTGAGAAGAA 10 0.25 No Hit GATACAAGATGAGGTTTGTGTATGCTCACTTTCCCATCAACGCTTCCATC 9 0.22499999999999998 No Hit GACATGATCAATTTGTTAGCCCCTGATGAAGATAAGGAGAAATACTCTCG 9 0.22499999999999998 No Hit ATCACTTCCAAAACAACGATCTCGAGTACGTAGTTGATGATCCTTTTGGA 8 0.2 No Hit CCAATAAGCATGACAAAATGGCAGCTATTCATGCAGATTGATCAGTCATT 8 0.2 No Hit AAAGGTTAAGCTGCTTGGGCTCAAAAAAGGAAACAGCAGATCTTAGTCAT 8 0.2 No Hit GGAAATGTGGTTGAAGTTAATGAAGAACTGAGCAGCTCTCCTGGTTTGGT 8 0.2 No Hit TGTGTTTCCGGCATCTACATTTGGCTATGCACGCGGGGTTGCTGCTGGAA 8 0.2 No Hit GAGCAGGTTTTGACACATCGATCAGGTTCTGCTGTTATGCTTGCACTTAT 8 0.2 No Hit ACAGTGGATACCTGGAAAGAGCATTTCGAGAAGGGAAAAGGGTCTCAGAA 7 0.17500000000000002 No Hit AACTCAGAAAAGTCCCATTTTAATTCTTCAATAAAAAAAGGCGTCATGTC 7 0.17500000000000002 No Hit GGAGATGACGAGCATTGCTGGACTGAGACCGGAGTGTCAAATATTGAAGG 7 0.17500000000000002 No Hit TGTCCAACCCGTTCTTTCCCGCCACCGACCTCACAAAGCATAAAAAAGGC 7 0.17500000000000002 No Hit AAAACCTTCTCCTCTCTCTGTCTCTCTGTGTCTTCAACCATGCAGCAGGC 7 0.17500000000000002 No Hit CACACACAACCCATCTTTCCCAGCTTCTTCTCCCAAACCAGAGAATCAGC 7 0.17500000000000002 No Hit AGTTAACATGAGCTGGTGGTGGTCTGGTGCTATTGGCGCTGCCAAGAAAA 7 0.17500000000000002 No Hit GATTCCTTGAAGACTGAATCGTTACTGGAGTGTTTGGCTGTTGTGACATT 7 0.17500000000000002 No Hit TTACAAACTAGAGTCACGGTTCCCTCCAGAAAACCTTAAGAAACGCCTTG 6 0.15 No Hit AAGAAAACATAAAAAGAAACAATCAACAGTACTCTCAGAACCGAAAGATG 6 0.15 No Hit GCTGCTATCCATTAGATGTTGTGAAAACCAGATTACAAGCTCAAACACCA 6 0.15 No Hit TAGCAACTGATCTTATATCCTCTTTGAGAGCTAAAGGAAAATGTGCACTA 6 0.15 No Hit GACTAGTGTTACTCACCTTTTCTCTATTACCAAGTTTCTTGGTCTTCTCG 6 0.15 No Hit GGGAGATATGGTGGCTTACCAACCTTATGCAATGGGCAGGATGAAGTTCA 6 0.15 No Hit AGAGTACAGGGCCCGCAGTTTCTCTTCTTGGATCTTTGTGCAGCTTTTCT 6 0.15 No Hit CGTGAAGGACAGCTCCTCCTCCTCATTTTAATTTCCCTGTACTCTCTGCC 6 0.15 No Hit TGCAAGAGGAAGGAAAGCAGCAGAGGAAGGAGGGAACAACAATGGAGGCT 6 0.15 No Hit ATCTCAGCTAAACCGACGCAGTTTTGCTCCTTGGATTCTGAGCCCGGGCA 6 0.15 No Hit CTTCTCATAAGCTAATCCCATATAGGCATTAGGCACCAGTACGACCCTTC 6 0.15 No Hit GTTTCGCTGGTGGGATAGAGTTCATGAGTTTATATTGTTAGGTGCTGTTC 6 0.15 No Hit AGCGATTTTGAGCTCCTGCTCCGTCCTCTCGCCTAAAACAACGATGGCAA 6 0.15 No Hit CATACATCCATAGAGAGAAAGAGAAGACATGGCAACCAGAACTCCAAAGC 6 0.15 No Hit GGTTTCTGTATATTTGCTGCTATTGCTCCTCCAATTGTCTTCCATGGGAA 6 0.15 No Hit AAGTCGAGCAATTGGGTATTTCTTGGAGCCATTGGTTGTTCTTGGGTTGT 6 0.15 No Hit CACAAGCTTACGAACGCTCTTCTTCCTGAAACGAAAGACTGGAGGGAAGA 6 0.15 No Hit GCACTTGGGGCTGATTCACTTGATACGGTTGAGATTGTGATGGGACTTGA 6 0.15 No Hit GTTTGCAAAGCTCAGGGTTTTGTGTATGGGATCATTCCTGAGAAGGGAAA 6 0.15 No Hit CATCTTCTGGGTTTTTGTTTCCAAATGGTGGTTCAAGGAGAGTGTGATGA 6 0.15 No Hit AAAATAAAATGGAATCGAAAGAGAAAAAATAAATAAATAAATAGAGTGTT 5 0.125 No Hit CAATAACCAACTGGTTCAAGATTGAGAAGGCAGGTGATGATTACAAGCTA 5 0.125 No Hit GCTTGAATTCCTTGGTAATGAGTCGTGCATAAGAGTCTGCTTCATCTGTC 5 0.125 No Hit GGATTTGATTGAGTTGAAAGGGAAAATTTAAGGAGGCTGCAGTGTGATGA 5 0.125 No Hit CATTATTTCTGGCCAAAGTCACAAGAAACTATGGAGGCTGGTTCCTGGAG 5 0.125 No Hit GGGGCCTGTGAGAAAAGCTTTGAAGTACCTTTCTTGGTGCAAGATGGACT 5 0.125 No Hit GGAAGATATCGAAGAGAAAGTAGTTGAAAGCCTGAAAAAAGAATATATGA 5 0.125 No Hit CAGAAGAAGGGATTGAGGCCTGTCATGCTATTGCTTCTTTATGTGAAGTT 5 0.125 No Hit GAAATGGCGCAGATTTTGTTGCACGGGAATTTACATGTGACGATCTATGA 5 0.125 No Hit AACACGAACCATTAAAAAGTACAGTAAACAAGAGCAGATTTTGTCAAGAT 5 0.125 No Hit AGAAGATTGTGGTAGTGGACAGTGCAATGCCTAATGGAGATTCCCAGCGG 5 0.125 No Hit TAGAGGCCCTCTCTCAGTGAATGCTCGGCCACCAAAATACTCAATTGCCA 5 0.125 No Hit CTTCTCCCCATCTTATGTGATTCCTCACGGAAAAAAGCAATCTCTTATCT 5 0.125 No Hit GTGGTATCTGATCGTGTGGTGGACTCTCCCTGCTGCTTGGTCACTGGAGA 5 0.125 No Hit GTGTCATTCAGCTTTTATATCCCCTATTCTCTTCGTGAAGATGTCTTGCT 5 0.125 No Hit GGACAGGATCCGAGTGCTCCTGTGGAGATAGCCGATTTGTGTGAGGGTAT 5 0.125 No Hit CACACTTTGTGCGCAACAATTTCTACAACGACTTGATTGAGAAATTGGTA 5 0.125 No Hit AATTTTTCTTAGCGCCAGTTAATGTCTGAATCTTGACTGGCACTTTCAGC 5 0.125 No Hit AATTCCTATCCCTCGTCAACCAGGGAGCGTGAAGTTTGATTGATTGTTTT 5 0.125 No Hit TGCAATGATGCTGATCAGGACATTGGGGACGTTGGTTCCCCAAAGCTGAG 5 0.125 No Hit TACAGTACCAATCCATTCGTCTGGTTAGGCACCTTGAATGAGATGCAATG 5 0.125 No Hit AGAAACTTAGGAAAGAAAGAGGAGAGAGAGACCACTTCACTATCTAAAAA 5 0.125 No Hit GTGAGGATGCCGAGAAGTTCCCGACACAAATCGAGCAAGCATAGCTCGAG 5 0.125 No Hit GTTGTCACATTGATGATGAGCATACCATGGAATCCAAGAATGGGCGCAAG 5 0.125 No Hit CACAAATCGAGAAGTTAAAGGCTAGAATAGATATGATTGCAGCAGCCTGT 5 0.125 No Hit GAAGAAGTTTGCCGCTTTTAGGGCTTTTGCAGGACAGTCTTCAATTTCTA 5 0.125 No Hit GTTTTGCCTTTGGAACCCATCCATCCATCAGGAAGGAAGGATCAGGTCAA 5 0.125 No Hit TATCTGCAGCTTTTCGGGCAGCATTCTGCTTCAATTTGGGAGACAATGTT 5 0.125 No Hit TTGACATGCCTGGAATTGGAAGCAAAGGAGTGAGGGTGTGGTTTGAAAAC 5 0.125 No Hit GGAGGAGGAGGAGGGATATTGGAGAAGCCCGTTATCGAGAGAACCACTCC 5 0.125 No Hit GTTTTACTAGACAAGATGTGGGATGATGTTGTTGCTGGACCTCAGCCAGA 5 0.125 No Hit CTAATCTTCCCTCTCTCTCTCTCTAAAAATAACCAAAAAAACAAGTTACT 5 0.125 No Hit AGAACTCTCAGTGATCTGAATGGAAATGCAGAGCTGTTGGGTGAGCAGAA 5 0.125 No Hit GTTGAAGGTTTCGCCTAAAGCTGTGTTAAATAGGCCAGAGAGAGAGTTGA 5 0.125 No Hit ACGCAATTTTACTCAAGTGGATACTGCATGACTGGAGTGATGAAGACTGC 5 0.125 No Hit AAACGCTTGAGTTCAATGTTGGTAAGCCATCTAATGTTTTTGGGGACATG 5 0.125 No Hit CAACACTCGTGAGCCACCCAGCGAGACCACCGATGATGTTTTCAATCACG 5 0.125 No Hit GATAAGCTAGGCAAGATGGTTTTACTAGACAAGATGTGGGATGATGTTGT 5 0.125 No Hit GTTCTGTTGCACTGTCTCAAACCACCAAAGTGGAAATAGATGATCTCATA 5 0.125 No Hit GAACAGAGAAGCAAAAGCAACCGCTTCATTCTTTCCTTCCTTCATCAATT 5 0.125 No Hit GCGAAAGCAAAATGAAAATGAAGAGAGTCTTGGGAACAATTACCATCCAT 5 0.125 No Hit AGGAAGCATGAACCTCATGGTTAACTTGGCTCACCACAAGCTTGCTGCTG 5 0.125 No Hit CCTGCACCAGTGCTGCCACCACCGACTCCAAAGAAGCCGCCATCAGGTTA 5 0.125 No Hit CCTGATTGTTTATGGAACCGAAGGTGCTAAGATGGTTAAGGCCTTCCGTA 5 0.125 No Hit GGCAGCTCCTGATAAAATCTCAACCTGGGGAAGTAGTCAAACCTGATCAA 5 0.125 No Hit GCAGAGGAGGAGACAAAGAAGGTGGAAACTGAAACCCCATCAGAGACAAC 5 0.125 No Hit GGGTGATACGGAGGATGCTAATAGTGAAATGATACACAGGTTGCAATCTT 5 0.125 No Hit AAATATTTACCGACTGCTTTAATTGTTTGCCCGTTTCTGCACTGATTGAT 5 0.125 No Hit CTCCACTACTTCCTGCTTTTACTCCTACTGTTTATCTCTGTGAATCATGG 5 0.125 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.025 0.0 0.0 0.0 0.0 50-51 0.025 0.0 0.0 0.0 0.0 52-53 0.025 0.0 0.0 0.0 0.0 54-55 0.025 0.0 0.0 0.0 0.0 56-57 0.025 0.0 0.0 0.0 0.0 58-59 0.025 0.0 0.0 0.0 0.0 60-61 0.025 0.0 0.0 0.0 0.0 62-63 0.025 0.0 0.0 0.0 0.0 64-65 0.025 0.0 0.0 0.0 0.0 66-67 0.025 0.0 0.0 0.0 0.0 68-69 0.025 0.0 0.0 0.0 0.0 70-71 0.025 0.0 0.0 0.0 0.0 72-73 0.05 0.0 0.0 0.0 0.0 74-75 0.1 0.0 0.0 0.0 0.0 76-77 0.15 0.0 0.0 0.0 0.0 78-79 0.175 0.0 0.0 0.0 0.0 80-81 0.2 0.0 0.0 0.0 0.0 82-83 0.21250000000000002 0.0 0.0 0.0 0.0 84-85 0.25 0.0 0.0 0.0 0.0 86-87 0.3125 0.0 0.0 0.0 0.0 88-89 0.4625 0.0 0.0 0.0 0.0 90-91 0.4875 0.0 0.0 0.0 0.0 92-93 0.5875 0.0 0.0 0.0 0.0 94-95 0.725 0.0 0.0 0.0 0.0 96-97 0.925 0.0 0.0 0.0 0.0 98-99 1.1749999999999998 0.0 0.0 0.0 0.0 100-101 1.4 0.0 0.0 0.0 0.0 102-103 1.5499999999999998 0.0 0.0 0.0 0.0 104-105 1.75 0.0 0.0 0.0 0.0 106-107 1.9375 0.0 0.0 0.0 0.0 108-109 2.1375 0.0 0.0 0.0 0.0 110-111 2.3 0.0 0.0 0.0 0.0 112-113 2.55 0.0 0.0 0.0 0.0 114-115 2.625 0.0 0.0 0.0 0.0 116-117 3.0875000000000004 0.0 0.0 0.0 0.0 118-119 3.525 0.0 0.0 0.0 0.0 120-121 3.6875 0.0 0.0 0.0 0.0 122-123 4.0625 0.0 0.0 0.0 0.0 124-125 4.6625 0.0 0.0 0.0 0.0 126-127 5.262499999999999 0.0 0.0 0.0 0.0 128-129 5.8125 0.0 0.0 0.0 0.0 130-131 6.2625 0.0 0.0 0.0 0.0 132-133 7.075 0.0 0.0 0.0 0.0 134-135 7.85 0.0 0.0 0.0 0.0 136-137 8.55 0.0 0.0 0.0 0.0 138-139 9.275 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content pass >>END_MODULE Read 873936 spots for SRR26075386.sra Written 873936 spots for SRR26075386.sra Read 873936 spots for SRR26075386.sra Written 873936 spots for SRR26075386.sra Read 873936 spots for SRR26075386.sra Written 873936 spots for SRR26075386.sra Read 873936 spots for SRR26075386.sra Written 873936 spots for SRR26075386.sra Read 873936 spots for SRR26075386.sra Written 873936 spots for SRR26075386.sra Read 873936 spots for SRR26075386.sra Written 873936 spots for SRR26075386.sra Read 873936 spots for SRR26075386.sra Written 873936 spots for SRR26075386.sra Read 873936 spots for SRR26075386.sra Written 873936 spots for SRR26075386.sra Read 873936 spots for SRR26075386.sra Written 873936 spots for SRR26075386.sra Read 873936 spots for SRR26075386.sra Written 873936 spots for SRR26075386.sra Read 873936 spots for SRR26075386.sra Written 873936 spots for SRR26075386.sra Read 873936 spots for SRR26075386.sra Written 873936 spots for SRR26075386.sra Read 873936 spots for SRR26075386.sra Written 873936 spots for SRR26075386.sra Read 873936 spots for SRR26075386.sra Written 873936 spots for SRR26075386.sra Read 873936 spots for SRR26075386.sra Written 873936 spots for SRR26075386.sra Read 873936 spots for SRR26075386.sra Written 873936 spots for SRR26075386.sra Read 873936 spots for SRR26075386.sra Written 873936 spots for SRR26075386.sra Read 873947 spots for SRR26075386.sra Written 873947 spots for SRR26075386.sra Read 873936 spots for SRR26075386.sra Written 873936 spots for SRR26075386.sra Read 873936 spots for SRR26075386.sra Written 873936 spots for SRR26075386.sra SRR ids: ['SRR26075386.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_z539mvo2 SRR26075386.sra spots: 17478731 blocks: [[1, 873936], [873937, 1747872], [1747873, 2621808], [2621809, 3495744], [3495745, 4369680], [4369681, 5243616], [5243617, 6117552], [6117553, 6991488], [6991489, 7865424], [7865425, 8739360], [8739361, 9613296], [9613297, 10487232], [10487233, 11361168], [11361169, 12235104], [12235105, 13109040], [13109041, 13982976], [13982977, 14856912], [14856913, 15730848], [15730849, 16604784], [16604785, 17478731]] SRR26075386 file size 6449203 SRR26075386 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o SRR26075386 SRR26075386_1.fastq SRR26075386_2.fastq Input file: SRR26075386_1.fastq Paired file: SRR26075386_2.fastq trimmed: SRR26075386-trimmed-pair1.fastq, SRR26075386-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Wed Feb 12 00:49:21 2025 >> started Wed Feb 12 00:49:42 2025 >> done (21.005s) 17478731 read pairs processed; of these: 77 ( 0.00%) short read pairs filtered out after trimming by size control 14959 ( 0.09%) empty read pairs filtered out after trimming by size control 17463695 (99.91%) read pairs available; of these: 2394432 (13.71%) trimmed read pairs available after processing 15069263 (86.29%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 18 4 0.00% 19 5 0.00% 20 4 0.00% 21 5 0.00% 22 6 0.00% 23 12 0.00% 24 23 0.00% 25 7 0.00% 26 24 0.00% 27 18 0.00% 28 16 0.00% 29 14 0.00% 30 21 0.00% 31 21 0.00% 32 21 0.00% 33 20 0.00% 34 31 0.00% 35 25 0.00% 36 30 0.00% 37 29 0.00% 38 29 0.00% 39 26 0.00% 40 38 0.00% 41 23 0.00% 42 37 0.00% 43 25 0.00% 44 35 0.00% 45 57 0.00% 46 46 0.00% 47 57 0.00% 48 44 0.00% 49 55 0.00% 50 74 0.00% 51 69 0.00% 52 102 0.00% 53 97 0.00% 54 73 0.00% 55 113 0.00% 56 130 0.00% 57 118 0.00% 58 145 0.00% 59 204 0.00% 60 219 0.00% 61 253 0.00% 62 245 0.00% 63 325 0.00% 64 352 0.00% 65 382 0.00% 66 420 0.00% 67 414 0.00% 68 580 0.00% 69 638 0.00% 70 726 0.00% 71 837 0.00% 72 1052 0.01% 73 1203 0.01% 74 1349 0.01% 75 1499 0.01% 76 1644 0.01% 77 1986 0.01% 78 2090 0.01% 79 2413 0.01% 80 2618 0.01% 81 2995 0.02% 82 3334 0.02% 83 4022 0.02% 84 4393 0.03% 85 5184 0.03% 86 5413 0.03% 87 5586 0.03% 88 6264 0.04% 89 6579 0.04% 90 7274 0.04% 91 8255 0.05% 92 8777 0.05% 93 9974 0.06% 94 10836 0.06% 95 11735 0.07% 96 12750 0.07% 97 13493 0.08% 98 14286 0.08% 99 14858 0.09% 100 15700 0.09% 101 16333 0.09% 102 17743 0.10% 103 18519 0.11% 104 20147 0.12% 105 21605 0.12% 106 22701 0.13% 107 23670 0.14% 108 24087 0.14% 109 25139 0.14% 110 25735 0.15% 111 27263 0.16% 112 28494 0.16% 113 29131 0.17% 114 31171 0.18% 115 31992 0.18% 116 33015 0.19% 117 34786 0.20% 118 35506 0.20% 119 36549 0.21% 120 36859 0.21% 121 38663 0.22% 122 38882 0.22% 123 40580 0.23% 124 42261 0.24% 125 43708 0.25% 126 44633 0.26% 127 45968 0.26% 128 48025 0.27% 129 48294 0.28% 130 49765 0.28% 131 49663 0.28% 132 51045 0.29% 133 52772 0.30% 134 53043 0.30% 135 53869 0.31% 136 57044 0.33% 137 56596 0.32% 138 58739 0.34% 139 59791 0.34% 140 60524 0.35% 141 62061 0.36% 142 62593 0.36% 143 63427 0.36% 144 65493 0.38% 145 66191 0.38% 146 66520 0.38% 147 68742 0.39% 148 69678 0.40% 149 69371 0.40% 150 71160 0.41% 151 15069263 86.29% 17463695 reads passed initial QC criterion=sequence-density sequence-density=0.18 sequence-density-rank=1 fanout-score=7.06 fanout-score-rank=19 prefix-density=0.38 prefix-fanout=3.3 sequence=TCCACACTTGCAGCCATTCTCAGCACCAAAGTTCATCTCAGACC criterion=fanout-score sequence-density=0.03 sequence-density-rank=31 fanout-score=121.60 fanout-score-rank=1 prefix-density=0.31 prefix-fanout=11.8 sequence=CATCACCATCCCTGATTGATCTTTGATTCACAACAAGACCAACCAACCGTACAATGTTTACATAACTTGGGATAATCTCACAAGAAAGAAAGATCAATACAAAACTAGTCAGCTCAGGATGTTCCAGGCACGGTAGAACCGTAGAACCATAACAACAAGAGACATATTGCAGATGAGTACTGAAAAACAAAACACAGTACGTATTTACATGGGCAACCTTGGTTGAAGGCAACCTCATCAACGATGCTCGCTCTTCGTCTCTCCACTGTACATCCAGTCATAGACAGTGGGTGTGTTAGGCTGTGGCTTGTCAAAAACATGAGCACCAATATTCTTAGTAGCAAGGTTGCTACCAGGGTGGAACACGCTCCTCCAAACATTGCTACGCGCCGACACTGGGGTTGTAGGGGTCACTGGTGTCGTCGGTGTCCCTGGAGTTCCTGGCATAGTCATGGACCTCTGAAACTTATTAACAGGACTGCTCCCCTCTCCGACGTCAATATCTTTGATG criterion=sequence-density sequence-density=0.32 sequence-density-rank=1 fanout-score=2.56 fanout-score-rank=36 prefix-density=0.32 prefix-fanout=2.5 sequence=ATGTACCCTGAC criterion=fanout-score sequence-density=0.08 sequence-density-rank=26 fanout-score=289.87 fanout-score-rank=1 prefix-density=0.93 prefix-fanout=23.7 sequence=AGAAGAAGAGAGG SRR26075386 testing PE reads STAR mapping to Ensembl genome Started job on | Feb 12 00:50:20 Started mapping on | Feb 12 00:50:20 Finished on | Feb 12 00:52:54 Mapping speed, Million of reads per hour | 408.24 Number of input reads | 17463695 Average input read length | 295 UNIQUE READS: Uniquely mapped reads number | 15819036 Uniquely mapped reads % | 90.58% Average mapped length | 293.92 Number of splices: Total | 14912195 Number of splices: Annotated (sjdb) | 14549877 Number of splices: GT/AG | 14632100 Number of splices: GC/AG | 215917 Number of splices: AT/AC | 13522 Number of splices: Non-canonical | 50656 Mismatch rate per base, % | 0.40% Deletion rate per base | 0.03% Deletion average length | 3.46 Insertion rate per base | 0.02% Insertion average length | 2.55 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 449932 % of reads mapped to multiple loci | 2.58% Number of reads mapped to too many loci | 84067 % of reads mapped to too many loci | 0.48% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 6.08% % of reads unmapped: other | 0.28% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 1194727 1194727 1194727 N_multimapping 449932 449932 449932 N_noFeature 445155 15662347 527763 N_ambiguous 173291 803 98715 UnstrandedReadsAssigned:15200590 PositiveStrandReadsAssigned:155886 NegativeStrandReadsAssigned:15192558 Dataset is classified negative stranded MeadianReadLen=151 20thPercentileLength=151 echo kmer=147 SRR26075386 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,400 [index] number of k-mers: 62,057,036 [index] number of equivalence classes: 130,681 [quant] running in paired-end mode [quant] will process pair 1: SRR26075386-trimmed-pair1.fastq SRR26075386-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 17,463,695 reads, 15,421,814 reads pseudoaligned [quant] estimated average fragment length: 219.016 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,034 rounds 52401 SRR26075386.ke.tsv 34699 SRR26075386.se.tsv 87100 total ==> SRR26075386.ke.tsv <== target_id length eff_length est_counts tpm Potri.005G200100.1.v4.1 2018 1799.98 1823 61.3605 Potri.005G024800.1.v4.1 1035 816.984 1058 78.459 Potri.004G059700.1.v4.1 961 742.984 0 0 Potri.007G009000.2.v4.1 1416 1197.98 0 0 Potri.003G141000.2.v4.1 2943 2724.98 714 15.8747 Potri.016G087400.1.v4.1 270 86.4235 1350.44 946.705 Potri.015G069301.1.v4.1 564 348.121 0 0 Potri.010G195200.1.v4.1 1773 1554.98 458 17.8447 Potri.012G127500.1.v4.1 977 758.984 10504 838.48 ==> SRR26075386.se.tsv <== Potri.001G166300.v4.1 0 Potri.001G448400.v4.1 72 Potri.001G233950.v4.1 0 Potri.001G122700.v4.1 163 Potri.001G212900.v4.1 0 Potri.001G182400.v4.1 0 Potri.001G256600.v4.1 0 Potri.001G040500.v4.1 6 Potri.001G416900.v4.1 0 Potri.001G452600.v4.1 961 SRR26075386 completed mapping pipeline successfully