Starting /dee2/code/volunteer_pipeline.sh SRR26075387 current disk space = 3051932876800 free memory = 1422172804 SRR26075387 SRAfilesize e9f0697f9ca3127a661a8a0b13658591 SRR26075387.sra SRR26075387.sra file validated SRR26075387 is paired end SRR26075387 is conventional basespace SRR26075387 read1 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR26075387_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.73875 37.0 37.0 37.0 37.0 37.0 2 36.625 37.0 37.0 37.0 37.0 37.0 3 36.6675 37.0 37.0 37.0 37.0 37.0 4 36.6915 37.0 37.0 37.0 37.0 37.0 5 36.7315 37.0 37.0 37.0 37.0 37.0 6 36.6345 37.0 37.0 37.0 37.0 37.0 7 36.6825 37.0 37.0 37.0 37.0 37.0 8 36.599 37.0 37.0 37.0 37.0 37.0 9 36.6445 37.0 37.0 37.0 37.0 37.0 10-14 36.66450000000001 37.0 37.0 37.0 37.0 37.0 15-19 36.5945 37.0 37.0 37.0 37.0 37.0 20-24 36.4933 37.0 37.0 37.0 37.0 37.0 25-29 36.445499999999996 37.0 37.0 37.0 37.0 37.0 30-34 36.3898 37.0 37.0 37.0 37.0 37.0 35-39 36.3418 37.0 37.0 37.0 37.0 37.0 40-44 36.2995 37.0 37.0 37.0 37.0 37.0 45-49 36.206 37.0 37.0 37.0 37.0 37.0 50-54 36.1591 37.0 37.0 37.0 37.0 37.0 55-59 36.029399999999995 37.0 37.0 37.0 37.0 37.0 60-64 36.028499999999994 37.0 37.0 37.0 37.0 37.0 65-69 35.9573 37.0 37.0 37.0 37.0 37.0 70-74 35.98870000000001 37.0 37.0 37.0 37.0 37.0 75-79 35.961 37.0 37.0 37.0 37.0 37.0 80-84 35.9952 37.0 37.0 37.0 37.0 37.0 85-89 35.8515 37.0 37.0 37.0 37.0 37.0 90-94 35.836400000000005 37.0 37.0 37.0 37.0 37.0 95-99 35.7389 37.0 37.0 37.0 37.0 37.0 100-104 35.773900000000005 37.0 37.0 37.0 37.0 37.0 105-109 35.6912 37.0 37.0 37.0 37.0 37.0 110-114 35.607899999999994 37.0 37.0 37.0 37.0 37.0 115-119 35.5613 37.0 37.0 37.0 37.0 37.0 120-124 35.5466 37.0 37.0 37.0 37.0 37.0 125-129 35.408500000000004 37.0 37.0 37.0 37.0 37.0 130-134 35.3494 37.0 37.0 37.0 34.6 37.0 135-139 35.1739 37.0 37.0 37.0 32.2 37.0 140-144 34.974900000000005 37.0 37.0 37.0 27.4 37.0 145-149 34.914100000000005 37.0 37.0 37.0 25.0 37.0 150-151 34.836 37.0 37.0 37.0 25.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.0 1101 2 0.0 1101 3 0.0 1101 4 0.0 1101 5 0.0 1101 6 0.0 1101 7 0.0 1101 8 0.0 1101 9 0.0 1101 10-14 0.0 1101 15-19 0.0 1101 20-24 0.0 1101 25-29 0.0 1101 30-34 0.0 1101 35-39 0.0 1101 40-44 0.0 1101 45-49 0.0 1101 50-54 0.0 1101 55-59 0.0 1101 60-64 0.0 1101 65-69 0.0 1101 70-74 0.0 1101 75-79 0.0 1101 80-84 0.0 1101 85-89 0.0 1101 90-94 0.0 1101 95-99 0.0 1101 100-104 0.0 1101 105-109 0.0 1101 110-114 0.0 1101 115-119 0.0 1101 120-124 0.0 1101 125-129 0.0 1101 130-134 0.0 1101 135-139 0.0 1101 140-144 0.0 1101 145-149 0.0 1101 150-151 0.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 17 1.0 18 1.0 19 1.0 20 3.0 21 3.0 22 4.0 23 7.0 24 9.0 25 12.0 26 9.0 27 19.0 28 19.0 29 21.0 30 24.0 31 28.0 32 63.0 33 122.0 34 175.0 35 449.0 36 2823.0 37 207.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 40.76019004751188 15.353838459614904 8.27706926731683 35.608902225556385 2 17.925 16.225 35.0 30.85 3 17.2 18.525 30.475 33.800000000000004 4 21.2 25.85 24.05 28.9 5 22.2 32.4 26.474999999999998 18.925 6 23.05 33.425 22.900000000000002 20.625 7 15.925 29.95 38.4 15.725 8 16.425 28.799999999999997 32.775 22.0 9 17.575 25.1 34.8 22.525000000000002 10-14 19.75197519751975 30.868086808680868 27.947794779477945 21.43214321432143 15-19 18.970000000000002 29.64 27.834999999999997 23.555 20-24 19.355 30.04 27.63 22.975 25-29 19.79 29.549999999999997 27.355 23.305 30-34 20.26 28.904999999999998 27.165 23.669999999999998 35-39 19.925 29.349999999999998 26.265 24.46 40-44 20.255000000000003 30.085 26.064999999999998 23.595 45-49 20.1 28.68 27.79 23.43 50-54 20.165 28.33 26.955000000000002 24.55 55-59 20.055 28.615000000000002 27.500000000000004 23.830000000000002 60-64 19.85 29.21 27.21 23.73 65-69 20.085 28.720000000000002 26.490000000000002 24.705 70-74 21.709999999999997 28.51 26.71 23.07 75-79 20.84 27.35 27.51 24.3 80-84 20.880000000000003 27.675 27.805000000000003 23.64 85-89 21.915000000000003 27.675 26.805 23.605 90-94 21.525 27.150000000000002 27.515 23.810000000000002 95-99 21.07 28.384999999999998 27.01 23.535 100-104 21.745 27.42 27.065 23.77 105-109 21.68 27.73 26.995 23.595 110-114 21.845 27.785 26.815 23.555 115-119 22.17 27.72 25.974999999999998 24.135 120-124 21.285 27.73 26.174999999999997 24.81 125-129 21.08 28.33 25.795 24.795 130-134 21.07 29.154999999999998 25.324999999999996 24.45 135-139 22.065 27.665 26.205000000000002 24.065 140-144 21.77 27.73 26.0 24.5 145-149 21.029999999999998 27.74 25.979999999999997 25.25 150-151 22.425 26.2125 25.4625 25.900000000000002 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.5 5 0.5 6 0.0 7 0.5 8 0.5 9 0.0 10 0.0 11 0.5 12 0.5 13 0.0 14 0.0 15 1.0 16 1.5 17 1.0 18 1.0 19 2.0 20 2.0 21 0.5 22 2.0 23 4.5 24 4.0 25 3.5 26 8.5 27 15.5 28 16.0 29 19.5 30 39.0 31 51.0 32 49.0 33 50.5 34 65.5 35 69.0 36 85.0 37 123.5 38 148.5 39 148.5 40 157.5 41 191.5 42 219.0 43 227.0 44 207.5 45 221.5 46 249.0 47 207.0 48 206.0 49 210.5 50 170.0 51 154.5 52 131.5 53 100.0 54 75.0 55 62.0 56 53.5 57 58.5 58 48.0 59 28.5 60 16.5 61 14.5 62 17.5 63 13.0 64 7.0 65 3.0 66 5.0 67 9.5 68 8.0 69 5.0 70 3.0 71 2.0 72 2.5 73 0.5 74 0.0 75 0.0 76 0.0 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.025 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.01 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150-151 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 61.575 #Duplication Level Percentage of deduplicated Percentage of total 1 63.74340235485181 39.25 2 21.599675192854242 26.6 3 8.526187576126674 15.75 4 3.126268777913114 7.7 5 1.705237515225335 5.25 6 0.8120178643930167 3.0 7 0.24360535931790497 1.05 8 0.08120178643930166 0.4 9 0.08120178643930166 0.44999999999999996 >10 0.08120178643930166 0.5499999999999999 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GATCGGAAGAGCACACGTCTGAACTCCAGTCACCATACCACATCTCGTAT 12 0.3 TruSeq Adapter, Index 22 (97% over 38bp) CATGGCTCTCCGGGTTGCATACTCTGGTGACATGTATAGAAGATCTCCTC 10 0.25 No Hit GCTCTTCGTCTCTCCACTGTACATCCAGTCATAGACAGTGGGTGTGTTAG 9 0.22499999999999998 No Hit CACTGGTTCTCCCTGCGCACCTCCTCTTCTTCCTCTGGAGTGAAGTCATT 9 0.22499999999999998 No Hit CCTCACTTAGTTACAGTTTTATGGATAATTGGGATATTCTTTGGTATAGT 8 0.2 No Hit GATCGGAAGAGCACACGTCTGAACTCCAGTCACCATACCACATCTCGTTT 8 0.2 TruSeq Adapter, Index 22 (97% over 38bp) GTTTTAACCCTAATAAGCTCCCGGCTTTATTCTGCCTACATAAACTTAAC 7 0.17500000000000002 No Hit CCAAGAACCAGAGTTTCGTTGGTGATCGTCTCTGAGGAGCTCATGTCAGG 7 0.17500000000000002 No Hit GCTATTAAAACCTTGAAAAGTATATTCAATATCCCTGTGGGATATTCTGA 7 0.17500000000000002 No Hit GACGTATCAAGCGTGGGCAGGTACCCGCTACCCACTAGCAACCGGCCACC 7 0.17500000000000002 No Hit GCCATAATTAAAATTTACAAAGGTACACACGAGCGTCCATGGATGTTTTA 7 0.17500000000000002 No Hit CTCCTGGAGCCTAAATGTGTCAACGATGTCAACAACCTTGGCAGCTTGGT 7 0.17500000000000002 No Hit GCCCTCCAAGCTCTTCCTTTTCAAATTTTCAAGAACTTTGTCCCTGCCAT 6 0.15 No Hit GCTCCGGGAAGCCATCGTCAAGCAACCTTGACGAATGTGACCATCTAATA 6 0.15 No Hit GGGCAGGTTCATTTTAAACGCGGTGACTAGGATGCTCATTTGAATGTCCC 6 0.15 No Hit ATGAGCTTTGTTTTCCCTACTAAAGGTTGTTATCGTTACATTTCCATCTG 6 0.15 No Hit GTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAACCAATGAGA 6 0.15 No Hit GTTATGTAACTTATCAAATACACCAGCACAAAATTCAAACTGACATAATT 6 0.15 No Hit TTTTTTTTACAGTCTGATTAAATTTAAGTCATCACCAACCCACAATCAAG 6 0.15 No Hit CACCAATAAAAATAAAAATTTGAAAATTAATAGAAGAAAAAATACATCCG 6 0.15 No Hit CTCATATCCAGGTTTTCTAATAAAATCTCATCTAACGGGCCGAGGATGCA 6 0.15 No Hit CTTGATGTTCCTAAAAGCAGCCTCACGAGCACCAGTGGTAAGGAGATACA 6 0.15 No Hit CCAGCAGAGTCTTTTAGCAGCTTCTCTAGTGCTGCAAACCCTTTGTTAAT 6 0.15 No Hit CAGAAATCAAAAGGGGATCCACATTGAACTAAGATCGCCACTCAAGAGGA 6 0.15 No Hit AGTAAAGATTTAGTAGAGTTCATAGTCCATCACAAATTGCTGCGACATGG 6 0.15 No Hit GCCCTTCCAACCCCATAAGCCCGCCATAACTGAGATTGGCTCTAACCACA 6 0.15 No Hit CTTTGAGATTCTCTTCTTCTTGTCAGATATTCTTGCACGCTTCCTTTGCA 6 0.15 No Hit CTCATCATTAATGTACACATCAAATTTCAAAGCTTTAGTTTTGTCAAACT 6 0.15 No Hit ATTGATGAAGCGCTTAGGAGTCTTTGTTTCACCTCGAGCCTGGTTCTTGA 6 0.15 No Hit ATTCACAACAAGACCAACCAACCGTACAATGTTTACATAACTTGGGATAA 6 0.15 No Hit CAAATTTAAGTTCCGCTTAGAAATCACCACCTCGAAGACGGAGCACGAGA 6 0.15 No Hit CCAACTCCCACAGGCGAATTCAAGCATCCAAATGTAGGTAAAAATATAGA 6 0.15 No Hit GGATTATCTTGGCAAATCATTTTGATTCGATTTGATAAATTAACATGTTC 5 0.125 No Hit CTCTTGTCAGCACCCTGTTTCTCTTTCTCTGCCTTCTCCTTCTCCTGCTT 5 0.125 No Hit TGCTTACAACCTTAGAGGTCGCCACATCTTCCAAGCACTTCCAAGTTAAA 5 0.125 No Hit CAGGAAATTAAAGAGAAAAAAATGGACAAACACTAAAGCCATAAGACGAC 5 0.125 No Hit CACATGATTAGGCCTTCCCAAAGAATCAAGCCCTTCAGTCCGGCTATGAA 5 0.125 No Hit GTAAAACGCAAGCACCGGCTGTCGAGTTGTACGGCCGTTCAGCCACGAGT 5 0.125 No Hit GCTAGATTGTGGACTCAGGCCAGCTGCTGCAGGGTTTGAAATTGGCAACT 5 0.125 No Hit GTGGAACAAGAGGAGCCTTCGTAAGGTAACTGAGAATAGTAGCAACAGGG 5 0.125 No Hit GAGGGAAACTATGGAAATAAATGGAGTATATATTGAAGATACATTTGCAG 5 0.125 No Hit CCCGTTGACTAAAGCCGTACGAACACCACTCGTTAACTCAATAGCAGATC 5 0.125 No Hit CTTGCCCACATCCATCAGTGCAATGCCCCTTGCAGACAAATCAGTCCTGA 5 0.125 No Hit GTCATAATATTTCACAATGGAATCAAACAACAACCGCAGTTAACAACAAT 5 0.125 No Hit ATTTCGAGGACACGTGTATCAAAATCGATGAATAAGGTCACAAATATGTG 5 0.125 No Hit GTCGAGGGGTCTTGGGCGTAGCCTTCTGGCCGCGTCTTCGATTATTCCTT 5 0.125 No Hit GTGGATGTTGTTGTTAGACACTTCTTTTTGTCTTTAAATATAAGGCGGGG 5 0.125 No Hit CAGGTTTTGTTTCCCTTAATTTTGAACCAAGCCTCTGCACATTGCTTATG 5 0.125 No Hit CTCCTCCTGTTTATATGAAGGCATGCAGCTTTTATCCTCGGAAAGATTTC 5 0.125 No Hit GGACAGGTCTCAGTTCTATTCCTCTTCCACACTCTACAATAGCATTAAAG 5 0.125 No Hit TTGACAAATCTAGTCAAATCCTTCTTGGGTGGAAGGGCACCACCAATTCC 5 0.125 No Hit GTAAGAGACTGGCCATGACATGGTTGCTGCATTTTTCTTTTGCGCGAGTT 5 0.125 No Hit ATCATCACAACCACTCTCCGTCTACAAGCATCCTACTAAAAAGACGCAAG 5 0.125 No Hit CCCTTAACCTCTCGGTTTCGCTTCTCGGCTCTCTGTCTCTCCTCCAGACT 5 0.125 No Hit GCCAAGAGCAATATCCTGAAGCTTCTCCCAACTAAGAGGACAGGTCTTGC 5 0.125 No Hit ATCAGCTTGCATTACATGCCTTCTCACTCTTGGGCTGGCGCTTCCAGCTA 5 0.125 No Hit AATCCGCAGGGTGCACAAAACATAAAACTTAAATTCCTTGGCAAATAAAA 5 0.125 No Hit GTCTCCGTAAGCTTTATTTCGAACGTAAAAATCAGAAGTAGAACTCTTCG 5 0.125 No Hit CTTTAGTTTTCTGTTGTAGAAAAAGAAATTGAAAGACCAAATGGCTCCTT 5 0.125 No Hit GGCCACACAAAGTTACAATCGGATCCTGAGCTAAGTCAAAGCAAATATTG 5 0.125 No Hit AGCCGTTACAGAGGTGCTGAGGTGACAGGAGGAGCTACACAAACATCATC 5 0.125 No Hit CACCTTTTTTCTGAGAACAAGAAACTTCTGTGTGCTCACAACAGTCATAT 5 0.125 No Hit CTCCGAGTTATCAGGCCTGAACTCGCGTTCACTTAGTTGTTACTTGGGCG 5 0.125 No Hit CCAGCATCATACATCAGAAACTAACTACTGCCAGGACTAAGTCTCTATTT 5 0.125 No Hit CTGTTGCTGGAGTTGGCTTGAGTAACTTTCCAGCCCAAGCAATTCCATCT 5 0.125 No Hit GCACAATATAATTTGAAATAAAACCAGACAACTTCTTGTTCCACTTTTAA 5 0.125 No Hit ATGAAAGGAGTAAATGGGCTAAGCATAAATTTCTTACTTCTCAAAATGGA 5 0.125 No Hit CAGTATAACGTAGACCACACCCAAAAAAAAAAAAGACTCATCGTATATAT 5 0.125 No Hit GCCTTCTCCTTGTGTTCAGCCCCTGGCGTTACCATGCTAGGCTTAAGTAG 5 0.125 No Hit CGCCAAGTTTGTGACACGGAAATTATGGGCGGACTCAATTTAACAGGTAT 5 0.125 No Hit GCTTTTAATCACACAACAAAAAATATTGAACAAACTTGCAAGTATTATGT 5 0.125 No Hit TCGTAACTGAGTCACAATGCGGACAGCATCAACCAAAATAGCAGCCTTGT 5 0.125 No Hit GTTAGTCATGAACAGCTTTCAACATGTGACCTAATACCATGCATGTGAAA 5 0.125 No Hit GCCTCACTTAGTTACAGTTTTATGGATAATTGGGATATTCTTTGGTATAG 5 0.125 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0 0.0 0.0 0.0 0.0 64-65 0.0125 0.0 0.0 0.0 0.0 66-67 0.075 0.0 0.0 0.0 0.0 68-69 0.075 0.0 0.0 0.0 0.0 70-71 0.0875 0.0 0.0 0.0 0.0 72-73 0.1 0.0 0.0 0.0 0.0 74-75 0.1 0.0 0.0 0.0 0.0 76-77 0.1375 0.0 0.0 0.0 0.0 78-79 0.175 0.0 0.0 0.0 0.0 80-81 0.2 0.0 0.0 0.0 0.0 82-83 0.325 0.0 0.0 0.0 0.0 84-85 0.4125 0.0 0.0 0.0 0.0 86-87 0.425 0.0 0.0 0.0 0.0 88-89 0.6 0.0 0.0 0.0 0.0 90-91 0.75 0.0 0.0 0.0 0.0 92-93 0.825 0.0 0.0 0.0 0.0 94-95 1.025 0.0 0.0 0.0 0.0 96-97 1.2625000000000002 0.0 0.0 0.0 0.0 98-99 1.525 0.0 0.0 0.0 0.0 100-101 1.8375 0.0 0.0 0.0 0.0 102-103 2.2750000000000004 0.0 0.0 0.0 0.0 104-105 2.7625 0.0 0.0 0.0 0.0 106-107 2.95 0.0 0.0 0.0 0.0 108-109 3.5 0.0 0.0 0.0 0.0 110-111 4.4125 0.0 0.0 0.0 0.0 112-113 4.85 0.0 0.0 0.0 0.0 114-115 5.25 0.0 0.0 0.0 0.0 116-117 5.8125 0.0 0.0 0.0 0.0 118-119 6.5625 0.0 0.0 0.0 0.0 120-121 7.15 0.0 0.0 0.0 0.0 122-123 7.9125 0.0 0.0 0.0 0.0 124-125 8.575 0.0 0.0 0.0 0.0 126-127 9.212499999999999 0.0 0.0 0.0 0.0 128-129 10.1875 0.0 0.0 0.0 0.0 130-131 10.9125 0.0 0.0 0.0 0.0 132-133 11.6125 0.0 0.0 0.0 0.0 134-135 12.7625 0.0 0.0 0.0 0.0 136-137 13.6375 0.0 0.0 0.0 0.0 138-139 14.325 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCCAATC 10 0.006830828 145.0 2 >>END_MODULE SRR26075387 read2 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR26075387_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.535 37.0 37.0 37.0 37.0 37.0 2 36.444 37.0 37.0 37.0 37.0 37.0 3 36.349 37.0 37.0 37.0 37.0 37.0 4 36.3755 37.0 37.0 37.0 37.0 37.0 5 36.299 37.0 37.0 37.0 37.0 37.0 6 36.272 37.0 37.0 37.0 37.0 37.0 7 36.3115 37.0 37.0 37.0 37.0 37.0 8 36.4685 37.0 37.0 37.0 37.0 37.0 9 36.2965 37.0 37.0 37.0 37.0 37.0 10-14 36.30459999999999 37.0 37.0 37.0 37.0 37.0 15-19 36.274 37.0 37.0 37.0 37.0 37.0 20-24 36.2051 37.0 37.0 37.0 37.0 37.0 25-29 36.1161 37.0 37.0 37.0 37.0 37.0 30-34 35.9371 37.0 37.0 37.0 37.0 37.0 35-39 35.833600000000004 37.0 37.0 37.0 37.0 37.0 40-44 35.7995 37.0 37.0 37.0 37.0 37.0 45-49 35.7645 37.0 37.0 37.0 37.0 37.0 50-54 35.725 37.0 37.0 37.0 37.0 37.0 55-59 35.7686 37.0 37.0 37.0 37.0 37.0 60-64 35.7726 37.0 37.0 37.0 37.0 37.0 65-69 35.6781 37.0 37.0 37.0 37.0 37.0 70-74 35.6369 37.0 37.0 37.0 37.0 37.0 75-79 35.5938 37.0 37.0 37.0 37.0 37.0 80-84 35.658500000000004 37.0 37.0 37.0 37.0 37.0 85-89 35.605900000000005 37.0 37.0 37.0 37.0 37.0 90-94 35.5558 37.0 37.0 37.0 37.0 37.0 95-99 35.612 37.0 37.0 37.0 37.0 37.0 100-104 35.559000000000005 37.0 37.0 37.0 37.0 37.0 105-109 35.50110000000001 37.0 37.0 37.0 37.0 37.0 110-114 35.3846 37.0 37.0 37.0 37.0 37.0 115-119 35.3617 37.0 37.0 37.0 37.0 37.0 120-124 35.2812 37.0 37.0 37.0 37.0 37.0 125-129 35.1796 37.0 37.0 37.0 34.6 37.0 130-134 35.1962 37.0 37.0 37.0 34.6 37.0 135-139 35.116200000000006 37.0 37.0 37.0 29.8 37.0 140-144 35.0476 37.0 37.0 37.0 27.4 37.0 145-149 35.028749999999995 37.0 37.0 37.0 25.0 37.0 150-151 34.735375000000005 37.0 37.0 37.0 25.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.0 1101 2 0.0 1101 3 0.0 1101 4 0.0 1101 5 0.0 1101 6 0.0 1101 7 0.0 1101 8 0.0 1101 9 0.0 1101 10-14 0.0 1101 15-19 0.0 1101 20-24 0.0 1101 25-29 0.0 1101 30-34 0.0 1101 35-39 0.0 1101 40-44 0.0 1101 45-49 0.0 1101 50-54 0.0 1101 55-59 0.0 1101 60-64 0.0 1101 65-69 0.0 1101 70-74 0.0 1101 75-79 0.0 1101 80-84 0.0 1101 85-89 0.0 1101 90-94 0.0 1101 95-99 0.0 1101 100-104 0.0 1101 105-109 0.0 1101 110-114 0.0 1101 115-119 0.0 1101 120-124 0.0 1101 125-129 0.0 1101 130-134 0.0 1101 135-139 0.0 1101 140-144 0.0 1101 145-149 0.0 1101 150-151 0.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 13 5.0 14 9.0 15 15.0 16 4.0 17 6.0 18 6.0 19 9.0 20 13.0 21 3.0 22 12.0 23 9.0 24 13.0 25 14.0 26 12.0 27 17.0 28 19.0 29 16.0 30 14.0 31 29.0 32 36.0 33 63.0 34 127.0 35 514.0 36 2769.0 37 266.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.275 22.6 11.200000000000001 21.925 2 32.300000000000004 24.6 26.1 17.0 3 23.974999999999998 29.325000000000003 28.349999999999998 18.35 4 27.474999999999998 31.175000000000004 21.8 19.55 5 28.875 34.325 19.625 17.175 6 24.825 35.425000000000004 22.25 17.5 7 25.0 21.85 34.9 18.25 8 26.025 26.650000000000002 24.525 22.8 9 26.0 26.075 26.575 21.349999999999998 10-14 26.474999999999998 28.999999999999996 23.990000000000002 20.535 15-19 25.540000000000003 27.744999999999997 25.865 20.849999999999998 20-24 24.855 28.935 25.380000000000003 20.830000000000002 25-29 25.869999999999997 27.139999999999997 26.33 20.66 30-34 25.85 28.044999999999998 25.135 20.97 35-39 24.975 27.62 26.384999999999998 21.02 40-44 25.314999999999998 27.965 25.650000000000002 21.07 45-49 25.235000000000003 28.095 25.805 20.865000000000002 50-54 23.775 28.194999999999997 27.525 20.505000000000003 55-59 25.2 27.395000000000003 27.175 20.23 60-64 25.05 28.1 25.945 20.905 65-69 25.180000000000003 27.71 26.85 20.26 70-74 24.895 28.08 26.845000000000002 20.18 75-79 24.065 28.345 26.779999999999998 20.810000000000002 80-84 24.315 28.425 26.555 20.705000000000002 85-89 24.425 28.405 26.75 20.419999999999998 90-94 24.945 27.810000000000002 27.450000000000003 19.794999999999998 95-99 25.435000000000002 28.62 26.779999999999998 19.165 100-104 25.3 27.82 26.745 20.135 105-109 25.240000000000002 27.665 25.665 21.43 110-114 24.959999999999997 28.610000000000003 26.645000000000003 19.785 115-119 26.115 28.49 26.025 19.37 120-124 25.915 28.005000000000003 26.169999999999998 19.91 125-129 25.314999999999998 28.53 26.424999999999997 19.73 130-134 25.69 28.77 27.12 18.42 135-139 25.535000000000004 28.865000000000002 26.700000000000003 18.9 140-144 26.31763176317632 28.762876287628764 25.757575757575758 19.161916191619163 145-149 26.31894784217633 28.43926588988348 26.483972595889384 18.75781367205081 150-151 26.790848856107015 27.203400425053132 27.51593949243655 18.4898112264033 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.5 3 0.5 4 0.0 5 0.5 6 0.5 7 0.0 8 0.5 9 1.0 10 1.0 11 2.0 12 1.5 13 2.0 14 2.5 15 0.5 16 0.5 17 1.0 18 1.5 19 2.0 20 1.0 21 2.0 22 3.5 23 3.0 24 2.5 25 1.5 26 0.5 27 1.0 28 3.5 29 8.5 30 9.5 31 6.0 32 16.5 33 23.0 34 31.0 35 43.5 36 64.0 37 85.5 38 100.5 39 118.5 40 165.5 41 209.5 42 229.5 43 246.5 44 247.0 45 272.5 46 290.5 47 275.5 48 240.0 49 205.5 50 176.5 51 154.5 52 148.5 53 130.0 54 102.5 55 66.0 56 56.0 57 50.5 58 32.5 59 27.5 60 18.0 61 11.5 62 9.0 63 10.0 64 7.0 65 3.0 66 2.5 67 2.0 68 2.0 69 2.5 70 2.5 71 3.0 72 1.5 73 1.0 74 1.5 75 0.5 76 1.5 77 2.0 78 2.5 79 2.5 80 0.5 81 0.5 82 1.0 83 1.0 84 2.0 85 1.5 86 1.0 87 2.0 88 2.0 89 1.5 90 2.5 91 5.5 92 5.5 93 2.5 94 2.5 95 2.0 96 0.5 97 0.5 98 0.5 99 0.5 100 4.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.01 145-149 0.015 150-151 0.0125 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 62.125 #Duplication Level Percentage of deduplicated Percentage of total 1 64.98993963782696 40.375 2 21.08651911468813 26.200000000000003 3 8.048289738430583 15.0 4 2.8973843058350104 7.199999999999999 5 1.5694164989939636 4.875 6 0.8450704225352111 3.15 7 0.2414486921529175 1.05 8 0.08048289738430583 0.4 9 0.08048289738430583 0.44999999999999996 >10 0.16096579476861167 1.3 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGT 16 0.4 No Hit GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG 16 0.4 No Hit AAGATTTACAATCTGCAGGGGAATAGCGAAGGGTTTGAAGTATCTTCACG 10 0.25 No Hit GGAGCGTGTTCCACCCTGGTAGCAACCTTGCTACTAAGAATATTGGTGCT 10 0.25 No Hit GTTTGTCAAGGTTGACCAAAATACCCTCTTTGACCTCATCCTGGCAGCTA 9 0.22499999999999998 No Hit TAATTAGGGCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATAGG 9 0.22499999999999998 No Hit GGACATTAATTAGGGCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCC 8 0.2 No Hit TGTAGACATTGGTGCAAATCCTTCAGCTGAAGGAGGTGATGAGGATGAGG 8 0.2 No Hit AGAGCGAGCATCGTTGATGAGGTTGCCTTCAACCAAGGTTGCCCATGTAA 7 0.17500000000000002 No Hit CAGGTTACCGGTGTTAGATTCGGCAACTCACAGGTTTCAATGGTTCCTGT 7 0.17500000000000002 No Hit GTCTCTTTCAGAAGGCATTGGTATCTTTTCCCCACTTCCAAGCATTTTTT 7 0.17500000000000002 No Hit GAGAGTTTGTATGTTGGCCCCAATGTTTTAAGTCTTAATCTATGCTCTGG 7 0.17500000000000002 No Hit TCAGCTTTTATATCCCCTATTCTCTTCGTGAAGATGTCTTGCTGTGGAGG 7 0.17500000000000002 No Hit ACTAATTAAGCTCAGTCAGTTCAAGGATTGCACGGTAGATTTGTAAACAA 7 0.17500000000000002 No Hit GTGATTCTGCTGATTGTAGGATTCACAGTGGCATTGGTGATGTTGTTCAA 6 0.15 No Hit GTATTTGGAAGAGAAGTACCCTCAGCATCCTTTGTTGCCTAGTGATCTTC 6 0.15 No Hit GGAGGGTTGCAGAAGCTCCAAAGAACGATAAATTCCAGTATACTCATTTT 6 0.15 No Hit GGAAGAATTAATCCTTACATGTCCAGCCCTTGCCACATTGAGTTGACTTT 6 0.15 No Hit GTATGGGGTTGATTGAATTGAGAAGGATTGAAGCATCGAGGGAAATCGCT 6 0.15 No Hit GTCAAACAGTATGTCCCAAGGGGACTTAAGCGCGGTGGCCTCCCCTATCC 6 0.15 No Hit ACTGCAGTCAGTCAACTATACCCACTCTCCTTTTGGCAACGAATCAATAT 6 0.15 No Hit GTCAACAGTGGGCCTCGTGAAGATGCTACTCGTATTGGCTCAGCTGGTGT 6 0.15 No Hit AAGGAAGAGCAGCTTCTTAGAGGAAATCCACTGTTGAATAATACAACCAC 6 0.15 No Hit CAACAACAACACAACAACAACCCAACAACACCAACCAACTTACAGGTATG 6 0.15 No Hit ATTTGTGTTGATATAGAAAACAATGGCACTACATGGAAAGATTGAGACAA 6 0.15 No Hit AGTCATTTTACATGGTTGGTGGTATTGAGGAGGTTATTGCCAAGGCTGAG 6 0.15 No Hit CACTCTTGAAGTTGAGAGCTCTGATACGATAGACAACGTGAAAGCCAAGA 6 0.15 No Hit ATTGCAGCTGAAAAGAAAAAGAACGCATTGACACCAATCACTGCTTTCCC 6 0.15 No Hit AGGAAATTCATTTTCAGCATTTTATTATCACATCAGAAGTTAGAAAAGGT 6 0.15 No Hit TCGGGAGCTTGCACTTGTTAGGCAGAGGAGTAAGGTGGAAGTCTTTTGAG 6 0.15 No Hit AGATAAGCTAGGCAAGATGGTTTTACTAGACAAGATGTGGGATGATGTTG 6 0.15 No Hit GCGGGTTATAGCTTTTCAGTCTCGACGGGCTAGCACACATCTGGTTGACT 6 0.15 No Hit AGAAACCCAAAATGCAAATACAGAAGCTGACAACAGGAAAAAGCAACCGA 6 0.15 No Hit TGGAGATAGCCCAGGTGATCCGCAGTCTAGATTTGACCATCCTGAAGGGC 6 0.15 No Hit CGGCTTACCAGGCGGTCTCTTTCCAAACAATGTCAAGTCCTACAGTCTTG 6 0.15 No Hit AGAAATCAGCAGCTGCTAAACCTAAACAGCCAAAGTCTATCAAGTCTCCT 5 0.125 No Hit GGACCATCCAATTGACAGGACCCTTGAAGTTGCTGAGAAGGTCTGGTCAG 5 0.125 No Hit ATTTGAAGAAGATGAAGTCAAACAGATGGAGACACGGGAGAAAAGAGATT 5 0.125 No Hit GGGATGCATTGTTAGTCAAGATCTCTCAGTTTTGAACTTGGTCATCGTAA 5 0.125 No Hit GGAAGAATGAGATTAATCTTAGTGCTTGCTCTTGCGTTTGCACTGCTGAA 5 0.125 No Hit GGTGGAGCAAGCTATTCTGAGGAGAATACCACTGTTGAAAACCAGTTACT 5 0.125 No Hit CAGGGAATGGTGAAGAGTTCGTTAACGAAGTGGGAACAATGGGAAGAATT 5 0.125 No Hit GGAAAGATTGAGACAACATTAGAACTCAAGTCCTCCGCAGAGAAGTTCTA 5 0.125 No Hit AGCATGGAAGACCTGGTATTGGTGCCACCCATTCTTCCAGGTTTATTCCC 5 0.125 No Hit AGCATCAAGGGCATGGATTGAGGAAAATGAGGGCAGCTCCTTGCTGGAAA 5 0.125 No Hit ACTCATTTGCAAAGTATCAGTGTGTCGACCTTCGTGTCAAAGATGAGTGA 5 0.125 No Hit GTAGTCGAGGCAAATGACAAGGATCTGCTTCTAGGTACTTCCGAGAAGGA 5 0.125 No Hit GCAGGACTAACAGAAGATGATGTGATGAGCCTGATTGAGGACAGAGCGGT 5 0.125 No Hit CGCATCTCTATCCAAAAAGGGACTTGGCTGGGTTTTGAGAGATGGCAAGT 5 0.125 No Hit AGGAATGGGAGTATTTGCACTTGTGGTAACGGTATTTGCATTATTGATGG 5 0.125 No Hit AGAAACTGACCGAGGAGTTAATTGCGTATGAGAATTACATGAAGTTTTAT 5 0.125 No Hit TCTCGATCTCCTCTTCCGCATGGCTCCCAAAAGAGGTGTGAAGGCCCCGT 5 0.125 No Hit GTTGGGGACTGTACAGTATGAGAAAATTAACATGTTCTCTCCAAGTGAAG 5 0.125 No Hit GCACAACACCTGTGAAGACTCCCTCTTGGCTGCACCAATCATCCTGGATT 5 0.125 No Hit GGAAACATTACAAGAGAGTTTAAAGAGGGGAAAAAAAATGTCATCTGATA 5 0.125 No Hit ATTTTTGAGGCAAGTTACACCTGTTGTTTGTGTTTGTTCATTTGTGTGCT 5 0.125 No Hit AGGGGATCTTCTGTGTCAGAGGTGGATCTGGAGTATGGGGCACCAGAGAT 5 0.125 No Hit GTTTGGAATTCAAGGTTGACAAACTATTTCAAATCCTCTCCTTGTTATCT 5 0.125 No Hit GTAGTGTGTCTAGCTCCAAAGCATGTAGGGAAAAGTTGCGAAGGGATAGG 5 0.125 No Hit GTCCGTTGGCCCTTGGATCATCACCTTTGCCGTTGCCTGCTACATCAACT 5 0.125 No Hit GGAGGAATGCACCTGGGTTATTGAGAGAGCTTTATCAGATGGATAAGTGT 5 0.125 No Hit GTTAAAATGGCTGCGGGATCGTTACCTGTTTTCTACCAAGATAAAAACAA 5 0.125 No Hit CTCTCTCTCCATATCAATCCCAGAAGGGGAATCACAAGTCGAAGATCTGA 5 0.125 No Hit CACCTTACCACTGTAGATGGTCAGGTCCTTTGGAGCAAGAGCCATGCAGG 5 0.125 No Hit ATTAGGGCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATAGGAG 5 0.125 No Hit GAGAGCTAGTGTTAGACAGGTTCTTGTTAGACAGAGAGATGGAGAAGAAA 5 0.125 No Hit GGATGTTCGGCGAAATGATGTACTCAAGGGTCTTCGGTAACTCGATAACC 5 0.125 No Hit ATCGGAGCACGAAGCTAATAATCGGTATGGAGGATGGTAACATGCAAGAG 5 0.125 No Hit GGAAGTAACTGTTCCACAAGTGAAGGGCGATCTGGTTCTTCAGCTTCATT 5 0.125 No Hit CAGAGATGGAAGCTGCCCGCATGGATTACGAACGCATTGGTGCTGATCTT 5 0.125 No Hit AACTCGTGGAGCTACTCGCTACTTCAGTGATGGCAAAGGTCGAGTTCTGA 5 0.125 No Hit GAATGGAGGTGGGGTCCAGAGTGATTCGGATTCATCATCAGTCCTTGATT 5 0.125 No Hit ATTCAAGAAAATAGTGGGAGGCTTTTATTTCCATTTGGTGAGCTGAAACA 5 0.125 No Hit CATGAAGAGCTCCTTAGCAAGGATGGGGTCTACACTGCACTAGTGAAGAG 5 0.125 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0 0.0 0.0 0.0 0.0 64-65 0.0125 0.0 0.0 0.0 0.0 66-67 0.075 0.0 0.0 0.0 0.0 68-69 0.075 0.0 0.0 0.0 0.0 70-71 0.0875 0.0 0.0 0.0 0.0 72-73 0.1 0.0 0.0 0.0 0.0 74-75 0.1 0.0 0.0 0.0 0.0 76-77 0.1375 0.0 0.0 0.0 0.0 78-79 0.175 0.0 0.0 0.0 0.0 80-81 0.2 0.0 0.0 0.0 0.0 82-83 0.325 0.0 0.0 0.0 0.0 84-85 0.4125 0.0 0.0 0.0 0.0 86-87 0.425 0.0 0.0 0.0 0.0 88-89 0.6 0.0 0.0 0.0 0.0 90-91 0.75 0.0 0.0 0.0 0.0 92-93 0.8375 0.0 0.0 0.0 0.0 94-95 1.075 0.0 0.0 0.0 0.0 96-97 1.3125 0.0 0.0 0.0 0.0 98-99 1.5875 0.0 0.0 0.0 0.0 100-101 1.875 0.0 0.0 0.0 0.0 102-103 2.3125 0.0 0.0 0.0 0.0 104-105 2.75 0.0 0.0 0.0 0.0 106-107 2.925 0.0 0.0 0.0 0.0 108-109 3.4749999999999996 0.0 0.0 0.0 0.0 110-111 4.4125 0.0 0.0 0.0 0.0 112-113 4.8625 0.0 0.0 0.0 0.0 114-115 5.25 0.0 0.0 0.0 0.0 116-117 5.8375 0.0 0.0 0.0 0.0 118-119 6.6125 0.0 0.0 0.0 0.0 120-121 7.2 0.0 0.0 0.0 0.0 122-123 7.9625 0.0 0.0 0.0 0.0 124-125 8.625 0.0 0.0 0.0 0.0 126-127 9.2625 0.0 0.0 0.0 0.0 128-129 10.2625 0.0 0.0 0.0 0.0 130-131 11.0125 0.0 0.0 0.0 0.0 132-133 11.725 0.0 0.0 0.0 0.0 134-135 12.825 0.0 0.0 0.0 0.0 136-137 13.7125 0.0 0.0 0.0 0.0 138-139 14.425 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTGGACC 10 0.006830828 145.0 6 TTGATAT 10 0.006830828 145.0 8 GTAGGGA 45 0.008957279 48.333332 145 >>END_MODULE Read 815485 spots for SRR26075387.sra Written 815485 spots for SRR26075387.sra Read 815488 spots for SRR26075387.sra Written 815488 spots for SRR26075387.sra Read 815485 spots for SRR26075387.sra Written 815485 spots for SRR26075387.sra Read 815485 spots for SRR26075387.sra Written 815485 spots for SRR26075387.sra Read 815485 spots for SRR26075387.sra Written 815485 spots for SRR26075387.sra Read 815485 spots for SRR26075387.sra Written 815485 spots for SRR26075387.sra Read 815485 spots for SRR26075387.sra Written 815485 spots for SRR26075387.sra Read 815485 spots for SRR26075387.sra Written 815485 spots for SRR26075387.sra Read 815485 spots for SRR26075387.sra Written 815485 spots for SRR26075387.sra Read 815485 spots for SRR26075387.sra Written 815485 spots for SRR26075387.sra Read 815485 spots for SRR26075387.sra Written 815485 spots for SRR26075387.sra Read 815485 spots for SRR26075387.sra Written 815485 spots for SRR26075387.sra Read 815485 spots for SRR26075387.sra Written 815485 spots for SRR26075387.sra Read 815485 spots for SRR26075387.sra Written 815485 spots for SRR26075387.sra Read 815485 spots for SRR26075387.sra Written 815485 spots for SRR26075387.sra Read 815485 spots for SRR26075387.sra Written 815485 spots for SRR26075387.sra Read 815485 spots for SRR26075387.sra Written 815485 spots for SRR26075387.sra Read 815485 spots for SRR26075387.sra Written 815485 spots for SRR26075387.sra Read 815485 spots for SRR26075387.sra Written 815485 spots for SRR26075387.sra Read 815485 spots for SRR26075387.sra Written 815485 spots for SRR26075387.sra SRR ids: ['SRR26075387.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_k7d19nth SRR26075387.sra spots: 16309703 blocks: [[1, 815485], [815486, 1630970], [1630971, 2446455], [2446456, 3261940], [3261941, 4077425], [4077426, 4892910], [4892911, 5708395], [5708396, 6523880], [6523881, 7339365], [7339366, 8154850], [8154851, 8970335], [8970336, 9785820], [9785821, 10601305], [10601306, 11416790], [11416791, 12232275], [12232276, 13047760], [13047761, 13863245], [13863246, 14678730], [14678731, 15494215], [15494216, 16309703]] SRR26075387 file size 6017129 SRR26075387 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o SRR26075387 SRR26075387_1.fastq SRR26075387_2.fastq Input file: SRR26075387_1.fastq Paired file: SRR26075387_2.fastq trimmed: SRR26075387-trimmed-pair1.fastq, SRR26075387-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Tue Feb 11 23:57:49 2025 >> started Tue Feb 11 23:58:13 2025 >> done (24.308s) 16309703 read pairs processed; of these: 65 ( 0.00%) short read pairs filtered out after trimming by size control 128073 ( 0.79%) empty read pairs filtered out after trimming by size control 16181565 (99.21%) read pairs available; of these: 3090540 (19.10%) trimmed read pairs available after processing 13091025 (80.90%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 18 11 0.00% 19 7 0.00% 20 11 0.00% 21 25 0.00% 22 11 0.00% 23 21 0.00% 24 29 0.00% 25 29 0.00% 26 25 0.00% 27 29 0.00% 28 28 0.00% 29 14 0.00% 30 27 0.00% 31 22 0.00% 32 17 0.00% 33 17 0.00% 34 31 0.00% 35 30 0.00% 36 29 0.00% 37 39 0.00% 38 28 0.00% 39 25 0.00% 40 27 0.00% 41 34 0.00% 42 34 0.00% 43 46 0.00% 44 52 0.00% 45 45 0.00% 46 50 0.00% 47 71 0.00% 48 55 0.00% 49 104 0.00% 50 88 0.00% 51 99 0.00% 52 102 0.00% 53 112 0.00% 54 136 0.00% 55 156 0.00% 56 133 0.00% 57 176 0.00% 58 237 0.00% 59 311 0.00% 60 245 0.00% 61 252 0.00% 62 370 0.00% 63 405 0.00% 64 464 0.00% 65 499 0.00% 66 500 0.00% 67 615 0.00% 68 760 0.00% 69 805 0.00% 70 987 0.01% 71 1165 0.01% 72 1219 0.01% 73 1586 0.01% 74 1727 0.01% 75 1924 0.01% 76 2043 0.01% 77 2454 0.02% 78 2664 0.02% 79 3027 0.02% 80 3382 0.02% 81 3917 0.02% 82 4471 0.03% 83 5174 0.03% 84 5598 0.03% 85 6355 0.04% 86 7115 0.04% 87 7630 0.05% 88 8253 0.05% 89 8647 0.05% 90 9937 0.06% 91 10485 0.06% 92 11771 0.07% 93 13368 0.08% 94 14285 0.09% 95 15114 0.09% 96 16239 0.10% 97 17868 0.11% 98 18149 0.11% 99 20052 0.12% 100 20690 0.13% 101 21288 0.13% 102 22971 0.14% 103 24263 0.15% 104 26315 0.16% 105 28234 0.17% 106 29857 0.18% 107 31130 0.19% 108 32285 0.20% 109 33648 0.21% 110 34197 0.21% 111 35713 0.22% 112 37789 0.23% 113 38643 0.24% 114 41068 0.25% 115 42949 0.27% 116 44042 0.27% 117 44936 0.28% 118 47726 0.29% 119 47830 0.30% 120 48608 0.30% 121 49422 0.31% 122 52621 0.33% 123 52577 0.32% 124 54866 0.34% 125 57152 0.35% 126 59489 0.37% 127 60325 0.37% 128 62626 0.39% 129 63408 0.39% 130 64662 0.40% 131 64739 0.40% 132 66245 0.41% 133 68240 0.42% 134 70142 0.43% 135 70257 0.43% 136 72018 0.45% 137 73502 0.45% 138 74876 0.46% 139 75607 0.47% 140 77039 0.48% 141 77122 0.48% 142 80061 0.49% 143 80062 0.49% 144 82266 0.51% 145 83534 0.52% 146 84448 0.52% 147 85261 0.53% 148 85329 0.53% 149 86561 0.53% 150 87807 0.54% 151 13091025 80.90% 16181565 reads passed initial QC criterion=sequence-density sequence-density=0.66 sequence-density-rank=1 fanout-score=2.11 fanout-score-rank=35 prefix-density=0.67 prefix-fanout=2.1 sequence=AGGAAACCTCCT criterion=fanout-score sequence-density=0.04 sequence-density-rank=27 fanout-score=60.26 fanout-score-rank=1 prefix-density=0.34 prefix-fanout=7.5 sequence=CATCACCATCCCTGATTGATCTTTGATTCACAACAAGACCAACCAACCGTACAATGTTTACATAACTTGGGATAATCTCACAAGAAAGAAAGATCAATACAAAACTAGTCAGCTCAGGATGTTCCAGGCACGGTAGAACCGTGGAACCATAACAACAAGAGACATATTGCAGATAAGTACTGAAAAACAAAACACAGTACGTATTTACATGGGCAACCTTGGTTGAAGGCAACCTCATCAACGATGCTCGCTCTTCGTCTCTCCACTGTACATCCAGTCATAGACAGTGGGTGTGTTAGGCTGTGGCTTGTCAAAAACATGAGCACCAATATTCTTAGTAGCAAGGTTGCTACCAGGGTGGAACACGCTCCTCCAAACATTGCTACGCGCCGACACTGGGGTTGTAGGGGTCACTGGTGTCGTCGGTGTCCCTGGAGTTCCTGGCATAGTCATGGACCTCTGAAACTTATTAACAGGACTGCTCCCCTCTCCGACGTCAATATCTTTGATG criterion=sequence-density sequence-density=0.38 sequence-density-rank=1 fanout-score=2.93 fanout-score-rank=24 prefix-density=0.38 prefix-fanout=2.9 sequence=ATGTACCCTGACTT criterion=fanout-score sequence-density=0.03 sequence-density-rank=27 fanout-score=39.28 fanout-score-rank=1 prefix-density=0.25 prefix-fanout=5.1 sequence=AACAACAACAACCAGGGGGTAACCCCAAACAACCAAACCCAGAGGTTCAATTCGATGGGAAGTTGTGGTTTTGGTGGTTGATGGAACCAGGATTTGAGGAACACGAACACGTTTGCACAGAGTGTGGGCATATCTATGCCCACTATCACCCCGGTGGGGAGGAGAACCACGCCCAAAGGCCATTTCAGTGTCCCAACACTGAATGTACTTGGTTCATCCTCCGGGGTTCGAGTCGGCGCCGCCCGACGCTCAGTGTGACCGTGCCTACCTCCGGCCTTGATTGTGTTGTTGTATCCCTAGCGGCGTACACCACGGACATGGATGCCAGCTCTAGCTACGGCTGGCTCCGACAGAAACTCACAGGCGGCACTCATTTGCAAAGTATCAGTGTGTCGACCTTCGTGTCAAAGATGAGTGAGTTATCTCGTGGTATGTCCAGAGTCACCGATGCTGTTGCCTTAGGGTATCAGCATCACTTTCAGGTGATACGGGGAAAATTAACATACTTGGG SRR26075387 testing PE reads STAR mapping to Ensembl genome Started job on | Feb 11 23:59:39 Started mapping on | Feb 11 23:59:39 Finished on | Feb 12 00:11:16 Mapping speed, Million of reads per hour | 83.58 Number of input reads | 16181565 Average input read length | 292 UNIQUE READS: Uniquely mapped reads number | 12640014 Uniquely mapped reads % | 78.11% Average mapped length | 291.36 Number of splices: Total | 9692209 Number of splices: Annotated (sjdb) | 9414320 Number of splices: GT/AG | 9501740 Number of splices: GC/AG | 138702 Number of splices: AT/AC | 11126 Number of splices: Non-canonical | 40641 Mismatch rate per base, % | 0.41% Deletion rate per base | 0.04% Deletion average length | 2.96 Insertion rate per base | 0.03% Insertion average length | 2.42 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 454200 % of reads mapped to multiple loci | 2.81% Number of reads mapped to too many loci | 94017 % of reads mapped to too many loci | 0.58% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 18.02% % of reads unmapped: other | 0.48% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 3087351 3087351 3087351 N_multimapping 454200 454200 454200 N_noFeature 380350 12473409 451986 N_ambiguous 167844 814 72548 UnstrandedReadsAssigned:12091820 PositiveStrandReadsAssigned:165791 NegativeStrandReadsAssigned:12115480 Dataset is classified negative stranded MeadianReadLen=151 20thPercentileLength=151 echo kmer=147 SRR26075387 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,400 [index] number of k-mers: 62,057,036 [index] number of equivalence classes: 130,681 [quant] running in paired-end mode [quant] will process pair 1: SRR26075387-trimmed-pair1.fastq SRR26075387-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 16,181,565 reads, 12,406,051 reads pseudoaligned [quant] estimated average fragment length: 200.109 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,154 rounds 52401 SRR26075387.ke.tsv 34699 SRR26075387.se.tsv 87100 total ==> SRR26075387.ke.tsv <== target_id length eff_length est_counts tpm Potri.005G200100.1.v4.1 2018 1818.89 773 23.6406 Potri.005G024800.1.v4.1 1035 835.891 777 51.7079 Potri.004G059700.1.v4.1 961 761.891 2 0.146023 Potri.007G009000.2.v4.1 1416 1216.89 0 0 Potri.003G141000.2.v4.1 2943 2743.89 369 7.48075 Potri.016G087400.1.v4.1 270 91.604 1845 1120.39 Potri.015G069301.1.v4.1 564 365.287 0 0 Potri.010G195200.1.v4.1 1773 1573.89 252 8.90659 Potri.012G127500.1.v4.1 977 777.891 12881 921.12 ==> SRR26075387.se.tsv <== Potri.001G166300.v4.1 0 Potri.001G448400.v4.1 50 Potri.001G233950.v4.1 0 Potri.001G122700.v4.1 490 Potri.001G212900.v4.1 0 Potri.001G182400.v4.1 0 Potri.001G256600.v4.1 0 Potri.001G040500.v4.1 0 Potri.001G416900.v4.1 0 Potri.001G452600.v4.1 436 SRR26075387 completed mapping pipeline successfully