Starting /dee2/code/volunteer_pipeline.sh SRR26075388 current disk space = 3049121906688 free memory = 1582127692 SRR26075388 SRAfilesize f232520796cd61a5048e0dc5ef2cef70 SRR26075388.sra SRR26075388.sra file validated SRR26075388 is paired end SRR26075388 is conventional basespace SRR26075388 read1 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR26075388_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.60275 37.0 37.0 37.0 37.0 37.0 2 36.623 37.0 37.0 37.0 37.0 37.0 3 36.657 37.0 37.0 37.0 37.0 37.0 4 36.733 37.0 37.0 37.0 37.0 37.0 5 36.7455 37.0 37.0 37.0 37.0 37.0 6 36.65 37.0 37.0 37.0 37.0 37.0 7 36.6695 37.0 37.0 37.0 37.0 37.0 8 36.6445 37.0 37.0 37.0 37.0 37.0 9 36.643 37.0 37.0 37.0 37.0 37.0 10-14 36.6266 37.0 37.0 37.0 37.0 37.0 15-19 36.6059 37.0 37.0 37.0 37.0 37.0 20-24 36.5822 37.0 37.0 37.0 37.0 37.0 25-29 36.4927 37.0 37.0 37.0 37.0 37.0 30-34 36.469899999999996 37.0 37.0 37.0 37.0 37.0 35-39 36.4479 37.0 37.0 37.0 37.0 37.0 40-44 36.402499999999996 37.0 37.0 37.0 37.0 37.0 45-49 36.3391 37.0 37.0 37.0 37.0 37.0 50-54 36.3565 37.0 37.0 37.0 37.0 37.0 55-59 36.2785 37.0 37.0 37.0 37.0 37.0 60-64 36.1879 37.0 37.0 37.0 37.0 37.0 65-69 36.1063 37.0 37.0 37.0 37.0 37.0 70-74 36.0647 37.0 37.0 37.0 37.0 37.0 75-79 36.1217 37.0 37.0 37.0 37.0 37.0 80-84 36.035700000000006 37.0 37.0 37.0 37.0 37.0 85-89 35.9666 37.0 37.0 37.0 37.0 37.0 90-94 35.9402 37.0 37.0 37.0 37.0 37.0 95-99 35.9575 37.0 37.0 37.0 37.0 37.0 100-104 35.8788 37.0 37.0 37.0 37.0 37.0 105-109 35.7966 37.0 37.0 37.0 37.0 37.0 110-114 35.722500000000004 37.0 37.0 37.0 37.0 37.0 115-119 35.5972 37.0 37.0 37.0 37.0 37.0 120-124 35.672 37.0 37.0 37.0 37.0 37.0 125-129 35.5017 37.0 37.0 37.0 37.0 37.0 130-134 35.412 37.0 37.0 37.0 37.0 37.0 135-139 35.2125 37.0 37.0 37.0 29.8 37.0 140-144 35.17960000000001 37.0 37.0 37.0 27.4 37.0 145-149 35.1809 37.0 37.0 37.0 29.8 37.0 150-151 34.934250000000006 37.0 37.0 37.0 25.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.0 1101 2 0.0 1101 3 0.0 1101 4 0.0 1101 5 0.0 1101 6 0.0 1101 7 0.0 1101 8 0.0 1101 9 0.0 1101 10-14 0.0 1101 15-19 0.0 1101 20-24 0.0 1101 25-29 0.0 1101 30-34 0.0 1101 35-39 0.0 1101 40-44 0.0 1101 45-49 0.0 1101 50-54 0.0 1101 55-59 0.0 1101 60-64 0.0 1101 65-69 0.0 1101 70-74 0.0 1101 75-79 0.0 1101 80-84 0.0 1101 85-89 0.0 1101 90-94 0.0 1101 95-99 0.0 1101 100-104 0.0 1101 105-109 0.0 1101 110-114 0.0 1101 115-119 0.0 1101 120-124 0.0 1101 125-129 0.0 1101 130-134 0.0 1101 135-139 0.0 1101 140-144 0.0 1101 145-149 0.0 1101 150-151 0.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 21 2.0 22 0.0 23 4.0 24 5.0 25 11.0 26 7.0 27 9.0 28 17.0 29 21.0 30 39.0 31 44.0 32 59.0 33 112.0 34 123.0 35 430.0 36 2906.0 37 211.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 41.37240170298021 13.498622589531681 9.391435011269722 35.73754069621838 2 21.224999999999998 15.325 35.0 28.449999999999996 3 20.200000000000003 18.5 25.624999999999996 35.675000000000004 4 21.2 24.3 24.65 29.849999999999998 5 25.674999999999997 29.599999999999998 24.425 20.3 6 22.400000000000002 33.1 21.95 22.55 7 15.5 28.525 38.75 17.224999999999998 8 17.150000000000002 27.150000000000002 33.275 22.425 9 19.725 21.175 36.3 22.8 10-14 20.18201820182018 29.27792779277928 27.53775377537754 23.002300230023 15-19 20.905 26.784999999999997 28.560000000000002 23.75 20-24 20.380000000000003 27.755000000000003 27.555000000000003 24.310000000000002 25-29 20.465 28.435 27.925 23.175 30-34 19.975 28.205000000000002 27.415 24.404999999999998 35-39 20.57 27.41 27.04 24.98 40-44 20.655 28.110000000000003 27.005000000000003 24.23 45-49 20.06 28.035 27.744999999999997 24.16 50-54 20.424999999999997 27.54 27.49 24.545 55-59 19.785 27.805000000000003 28.025 24.385 60-64 20.69 26.905 27.805000000000003 24.6 65-69 20.49 27.615000000000002 27.310000000000002 24.585 70-74 21.38 27.605 27.63 23.385 75-79 21.37 27.775 28.17 22.685 80-84 21.505 27.450000000000003 27.084999999999997 23.96 85-89 21.075 27.700000000000003 27.224999999999998 24.0 90-94 20.78 28.18 26.8 24.240000000000002 95-99 21.795 27.255000000000003 27.35 23.599999999999998 100-104 21.63 27.735 26.25 24.385 105-109 21.39 27.68 27.400000000000002 23.53 110-114 21.17 27.694999999999997 26.935 24.2 115-119 22.445 27.415 25.7 24.44 120-124 21.57 28.15 26.14 24.14 125-129 21.77 27.02 27.084999999999997 24.125 130-134 21.565 28.025 26.545 23.865 135-139 21.54 27.63 26.595000000000002 24.235 140-144 22.71 27.325 26.529999999999998 23.435 145-149 22.68 27.384999999999998 26.479999999999997 23.455000000000002 150-151 22.662499999999998 26.674999999999997 26.6625 24.0 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.5 11 1.0 12 0.5 13 0.5 14 1.0 15 0.5 16 2.0 17 2.0 18 0.0 19 0.0 20 0.5 21 1.0 22 1.0 23 0.5 24 0.5 25 1.0 26 3.0 27 4.5 28 8.5 29 16.5 30 19.0 31 18.5 32 23.0 33 33.0 34 61.5 35 81.0 36 85.0 37 110.0 38 118.0 39 117.5 40 141.5 41 181.0 42 226.5 43 242.0 44 264.5 45 244.5 46 195.5 47 223.0 48 237.5 49 231.5 50 206.0 51 158.0 52 146.0 53 130.5 54 107.5 55 91.5 56 66.0 57 40.5 58 39.0 59 29.5 60 12.5 61 16.0 62 16.0 63 8.5 64 3.0 65 5.0 66 7.0 67 4.5 68 2.5 69 4.0 70 2.5 71 0.5 72 2.0 73 1.5 74 0.0 75 0.0 76 0.0 77 0.0 78 0.0 79 0.5 80 0.5 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.17500000000000002 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.01 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150-151 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 57.8 #Duplication Level Percentage of deduplicated Percentage of total 1 61.20242214532872 35.375 2 20.804498269896193 24.05 3 9.126297577854672 15.825 4 4.930795847750865 11.4 5 2.292387543252595 6.625 6 0.6487889273356401 2.25 7 0.5622837370242215 2.275 8 0.17301038062283738 0.8 9 0.17301038062283738 0.8999999999999999 >10 0.08650519031141869 0.5 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GGGGATGGTAGCCAAATGAACCAAGAAGACTGCAAATCCAATGGGAAGGG 10 0.25 No Hit CCTTTCTCTTATCACTTTATTTCCTTTTGCAACATTGCTTATGCTAATCT 10 0.25 No Hit CACCGGAGCCTATTTCAGCTGTGCACACGATACTTTCAGGCGCAACACTC 9 0.22499999999999998 No Hit CCATGCCATCCCATAGCCAAATCATATCCACGGCTTTTAAGCTCGCGATA 9 0.22499999999999998 No Hit GTAAAACGCCGGATGGAGCCTGGGGCCGGAATGAGTCCGTCAAACAAATG 9 0.22499999999999998 No Hit GCGGATGCCGTGTCTTCTTCATCACTGTCCTCATTGAAGTAATCTTCCTC 9 0.22499999999999998 No Hit CTGCGATATATGTCGATGGTTTCCTCCCCAAGTGTTGTCGGAGGCCCAGG 8 0.2 No Hit AGCTGCACCGGTGAACTCTGCAAGACCCCTCTATTCATTGCCTTCCTACA 8 0.2 No Hit TGGAAGCCTTGAGGGAGAGTGAACAATGATTGCCACCCTGAGACCATGCA 8 0.2 No Hit TGGTGGTGTTGGAAAGTACTTGAAAGCAAAAAATGCTAAAACCGAATCCA 8 0.2 No Hit AGCATAGTTGTCGAATTGCCGCCCTTTTCTCCTCAGCCACAGCTGAAAGC 7 0.17500000000000002 No Hit AGGGGTTTCAAAGCAACAGCCCACTAAGCACGTGACCTATTGCGCACAAC 7 0.17500000000000002 No Hit GCACTGATTCCACACATAGCAAACTCTAGACAACTCCCATTCTGTCACAG 7 0.17500000000000002 No Hit CGATGCACCTATAATATCCATTTGGGTATGTCTCAGCTCCATTGACAAGG 7 0.17500000000000002 No Hit CACAGGCTCCTGCATCAGTACCAGAACATCCATAGGATCACTGTCCTCAC 7 0.17500000000000002 No Hit CACACCAATAGTTGGCTCGTATTTCTTCTCGAACTCTCCGGTAAGATGCC 7 0.17500000000000002 No Hit GTCTCCTTGCTATCTCCTCACCCCTGACCCCAGCAATTTAAGTACATAAC 7 0.17500000000000002 No Hit GTTCCAACTTTGAATCGAACACGACCATCAATCTTGATCATCAGCATCAC 7 0.17500000000000002 No Hit ATGAGATTTGAAGTTAAATAAAGGTGGGAGGAAACGCCCATTTGGCAGGC 7 0.17500000000000002 No Hit GGGACAACAAATTCTTCTCTTCCTCCTCTTTTCTAGTATGCAACCGATAC 7 0.17500000000000002 No Hit GTTAAAATGATTTTGGCCCGACGGAAACATAGATAAATTTGACTCAAGAT 7 0.17500000000000002 No Hit CCACCGTTCTATAGAACACAAGAATACTGCCTGCTGCCCTACTGGGAAGC 7 0.17500000000000002 No Hit CCCTTCTGGACATTGCAGAAAATGTTGCTTGTGTATGCTAGCCTGTCCAT 7 0.17500000000000002 No Hit GGGCAAATATGGAAGAATAAAGCGCCGTTATCCTGGAGAACAGGAGACAA 6 0.15 No Hit GCTTCCTTCATAGCATTCAAGAGATCAAGTGCAGCAACTGGATTGTTTGT 6 0.15 No Hit CTACGAAGCAACCCTTCTTTCATGCTTCATTATTCTTGGTTTCTTACACA 6 0.15 No Hit CTCTGATCACAACCTGGTGGTAAAGAGCTGCAAGTGCTGCTCCAATGAAG 6 0.15 No Hit ACGTGAAACTGCGGTGTGTAATTGTTAGAAGAACGTTCCAGAACTGGCGG 6 0.15 No Hit CCGTAATTTCAGCAGCATTCATAGCATTTGGAAGATCTTGCTTGTTAGCA 6 0.15 No Hit GTTCTCATCATCGAAGGAAACCTCCTCTTTAAAGACCCCGGCTTTCCCTC 6 0.15 No Hit GTCACCCTTTTCCCTGTCTGCATTGCTTGCTTCCCTCTCCAGCTCCTCCC 6 0.15 No Hit CCTTCAGCTTGGACGCATCCATCTCATTGTTGCTCCGGGGGGCAACTATC 6 0.15 No Hit GTCCCACAAGTCCCTGGTAGCTGTGCGGACACAGCTATATCCTCTTAATG 6 0.15 No Hit CCACTCTGCATGATGTAGTACAAGACGATTGCTTCATCAAAATCCAAGGT 6 0.15 No Hit GAATGGTGTCAAGCTAAAGCTCTGATTAGCTGGAAGGCTCCTAACATACC 6 0.15 No Hit GCTGTGTTGGTTTGTCATTGCAGTCAACAACGTAAGGCAGCGCACGCGGT 6 0.15 No Hit AGGGTCACTGGTGTCGTCGGTGTCCCTGGAGTTCCTGGCATAGTCATGGA 6 0.15 No Hit TATTCACATCGACCCAGCATCATACATCAGAAACTAACTACTGCCAGGAC 6 0.15 No Hit GGGCCTAGTTTGGTGACGGGAACCCATTTTTCTTCTTCCTCTTTGCGTCC 5 0.125 No Hit CGTCGGTGTCCCTGGAGTTCCTGGCATAGTCATGGACCTCTGAAACTTAT 5 0.125 No Hit GTGGGCTTATAGACCTTGTAAATTTTCATGACATCTCCTTCCAAACCATT 5 0.125 No Hit GGACGCGCAAATCCTCAAGTCCACCAGAACAAAAGATAGCAAAGCATTCA 5 0.125 No Hit CATACTTTCTCATTGGGAAGTATATTTCTCTCTTCTTCTCGCGCTCAGTC 5 0.125 No Hit GCGACGTTGAGGACGGTCCCATCTGTAATTTCCGTGAAGCGTTTTGGCAC 5 0.125 No Hit CTGCAAAGTTGAGTTACTCTTGCAAATGCAAGATGTCAACCACTATCTCC 5 0.125 No Hit GACCAGTCTCAGCTATGTTCCTCTCTGTATGACGGAATGCCCATATGATT 5 0.125 No Hit CACATCAGTTCATCTTGATCCCATTCAGCTCTCTTCGATTCACAATCATC 5 0.125 No Hit CATTATCTCTACATCGACCCCATGTCTGGCACAAATATCTTTAGCTTTAT 5 0.125 No Hit GGCTTCCTGTCTCGTCACGGTCCATTTTTTGCTTCTCCTGGTTAAACAAT 5 0.125 No Hit CTATAACACACAAAATAGCAATACACATGTAGAACATAGAATTCAGCCAG 5 0.125 No Hit CCGAGCCTTAAGCTGATCTTCGTAAGTGACAAACTGTTGTTGGAAGATCT 5 0.125 No Hit GTTTGGCACATCTCATGTTTTTCTCTCCCGAGGAGAGATGATATATGAGA 5 0.125 No Hit GTGCATAGCCCCATCTTCTCCTCCTGTAAAAATGAAATCCTCTGTATGGC 5 0.125 No Hit ACAGTGGTAGTACTTTCAGTTCATTGACATTGTTATGCTTCCATCATGAT 5 0.125 No Hit CCGGCACCAGAGTCCTCGGCATTTGCATCAGCAAAAGGATCATAAGCTGA 5 0.125 No Hit GTCAGTTTTGCCAAGCAGCCCGAGCCTTTTGGTGTAAGCTGCCAGACGGG 5 0.125 No Hit CCTGTCTATCCTTCGTCAGTCATTTCAAATGGGAGCTGCAAAAGTTGCTG 5 0.125 No Hit GTCTGCTGTTGTTGGTCGGACGATTAGTGGAGGAGGGGGATTGTGGTGGT 5 0.125 No Hit TGGCCACTTGCCGTAGTACTTCACTGGACGCTCGCCATTGGCACAGCGCG 5 0.125 No Hit CTTCAATCGCAGTACTTGACAGAACAGAAACAGTTGCCATATCAATAAAT 5 0.125 No Hit CCCACATCGAAGCATTGTTGACACGTCTTGAGCAGTCACCTGAAAGCTAC 5 0.125 No Hit TGGCTCTGCCCTGGAATCAGTTTCTGAGATCCTTTGCTTGATATCCCTCG 5 0.125 No Hit CGCAGAAGATGTGCCCATCGTTGTGTCTGAACTTTTTACCACCATAACAA 5 0.125 No Hit GTCCTGGAGACCCTCGGTCACACGAACAGACATGGTCTTCTCACCCTCAC 5 0.125 No Hit ATACAATAAGAGAAGAGTTTGTCGTATATATTAGCAGTTTGTGATGCTAA 5 0.125 No Hit ATACAGTTAGTGGAAGGATTGGACTCGGTGAGTTAGGGTTTTTTTTTTTT 5 0.125 No Hit GTCAGCTATCACCAGAATTAGCCACGGTGGGTGGCTGAGGCTGCTGGATG 5 0.125 No Hit GGAAGTTTTTTGGGCGCGGGAACAGTGCTGGAACAATGGTGGCGCGTTGC 5 0.125 No Hit AGAGCAGAATCTGATTGTTGATAAGCAGTCGATCTTGCCTTCGAAACTCT 5 0.125 No Hit CTGCTTTTAAAGAACAAGGTGGACTTTCCTGTCTCTTAGAATAGCTGGTT 5 0.125 No Hit GTCAAACACCTCGTTGAGCAGAGTGCCCTTCTTCATGCACATGATTCGCA 5 0.125 No Hit GGCCTCTTTCCTTTAACGGGATTCTCCTCCACCTTAGGCTCCTCCGTAGC 5 0.125 No Hit GTCTTGAGTATTTACGTGAGGGGTGCCGAGCCAGAATGGGTTGTGGCAAC 5 0.125 No Hit GCCGGAGGTAGGCACGGTCACACTGAGCGTCGGGCGGCGCCGACTCGAAC 5 0.125 No Hit TCTAGAGATAACCGATAGGAGACTCTTGTTATCGGAGGGGCCCATATCAA 5 0.125 No Hit CAGCTGTCGTATTGATCTTAGTTTCAGAGTCTCGAAGAGAAGCAGAGTCA 5 0.125 No Hit CAAGAAAAGCATAAGAGAGGAATCATAGAATAGAGCAAGAGGAAGAAGAT 5 0.125 No Hit GTCCTTCCATCTTCAAGCTGTTTTCCAGCAAAAATCAACCTCTGCTGGTC 5 0.125 No Hit CTACATACCATTACGAACAGCTACATAAATCAAATCAATAAAACAAATCA 5 0.125 No Hit GGGCATTCCTCATGCTTAGCTGGTTTTTCCTTTCCTCCCACTAACCTTGC 5 0.125 No Hit GGCTTATATCAGATCTCATCTAGCTTGTCTTTCCACACGGCATATCAGGT 5 0.125 No Hit ACCCGACATTGTCACCAGGAAGAGCCTCTAGGAGAGCCTCGTGGTGCATC 5 0.125 No Hit CTTGTATTTTGCTTCTTGCTCGGAGAAGTAGAACCCTTCGCAAGATTCTT 5 0.125 No Hit GGAGTTGTAATCATACTTTGGTTTCTCATCAACCCACAATTTCACCGCAG 5 0.125 No Hit TGAGGACTTGAGAAGTCATATGTCATGAACGAGAAACCATCTACATCATC 5 0.125 No Hit CGCGATATTTTCTTCTGTGAACCTTGGATGTGATTTCAGTACAGTCTCCG 5 0.125 No Hit GTTTGATAATCCTTTACAGCAGCCTTGATAGCATCCTCTGCAAGCATGCT 5 0.125 No Hit GTCCCGTTATTGTTGCTTGCCAGTGAAACATATCTTCAGCTACAGGGCCT 5 0.125 No Hit TCCCTATCACCACTTCCACTCCGTATTTCTCCACCGCTTTTGATAAAAGA 5 0.125 No Hit CACCTACAAGCGCTTTCTCATTGAGTTCGCCTTATTCTTCCAGAATGCCT 5 0.125 No Hit GCCTGTCAAAAGCATTGTGCCGTCGTCGTAAGGGAGAAACATCTTGCTCT 5 0.125 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.025 0.0 0.0 0.0 0.0 62-63 0.025 0.0 0.0 0.0 0.0 64-65 0.025 0.0 0.0 0.0 0.0 66-67 0.0625 0.0 0.0 0.0 0.0 68-69 0.1 0.0 0.0 0.0 0.0 70-71 0.1 0.0 0.0 0.0 0.0 72-73 0.1 0.0 0.0 0.0 0.0 74-75 0.1125 0.0 0.0 0.0 0.0 76-77 0.175 0.0 0.0 0.0 0.0 78-79 0.2375 0.0 0.0 0.0 0.0 80-81 0.275 0.0 0.0 0.0 0.0 82-83 0.275 0.0 0.0 0.0 0.0 84-85 0.325 0.0 0.0 0.0 0.0 86-87 0.375 0.0 0.0 0.0 0.0 88-89 0.475 0.0 0.0 0.0 0.0 90-91 0.625 0.0 0.0 0.0 0.0 92-93 0.8625 0.0 0.0 0.0 0.0 94-95 1.05 0.0 0.0 0.0 0.0 96-97 1.1 0.0 0.0 0.0 0.0 98-99 1.2875 0.0 0.0 0.0 0.0 100-101 1.45 0.0 0.0 0.0 0.0 102-103 1.5625 0.0 0.0 0.0 0.0 104-105 1.8375 0.0 0.0 0.0 0.0 106-107 2.1375 0.0 0.0 0.0 0.0 108-109 2.5 0.0 0.0 0.0 0.0 110-111 2.925 0.0 0.0 0.0 0.0 112-113 3.4124999999999996 0.0 0.0 0.0 0.0 114-115 3.825 0.0 0.0 0.0 0.0 116-117 4.1 0.0 0.0 0.0 0.0 118-119 4.387499999999999 0.0 0.0 0.0 0.0 120-121 4.9375 0.0 0.0 0.0 0.0 122-123 5.6375 0.0 0.0 0.0 0.0 124-125 6.0 0.0 0.0 0.0 0.0 126-127 6.2875 0.0 0.0 0.0 0.0 128-129 6.55 0.0 0.0 0.0 0.0 130-131 7.025 0.0 0.0 0.0 0.0 132-133 7.574999999999999 0.0 0.0 0.0 0.0 134-135 8.2 0.0 0.0 0.0 0.0 136-137 9.3375 0.0 0.0 0.0 0.0 138-139 10.162500000000001 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AGGGGAA 10 0.006830828 145.0 5 TCAGGGG 10 0.006830828 145.0 3 CAGGGGA 10 0.006830828 145.0 4 TTCAGGG 10 0.006830828 145.0 2 GGGGAAA 10 0.006830828 145.0 6 CCCCCCC 40 0.0076550315 18.125 90-94 >>END_MODULE SRR26075388 read2 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR26075388_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.0475 37.0 37.0 37.0 37.0 37.0 2 36.086 37.0 37.0 37.0 37.0 37.0 3 36.1095 37.0 37.0 37.0 37.0 37.0 4 36.2455 37.0 37.0 37.0 37.0 37.0 5 36.194 37.0 37.0 37.0 37.0 37.0 6 36.0465 37.0 37.0 37.0 37.0 37.0 7 36.166 37.0 37.0 37.0 37.0 37.0 8 36.185 37.0 37.0 37.0 37.0 37.0 9 36.18 37.0 37.0 37.0 37.0 37.0 10-14 36.1957 37.0 37.0 37.0 37.0 37.0 15-19 36.147800000000004 37.0 37.0 37.0 37.0 37.0 20-24 36.085899999999995 37.0 37.0 37.0 37.0 37.0 25-29 35.9919 37.0 37.0 37.0 37.0 37.0 30-34 35.9194 37.0 37.0 37.0 37.0 37.0 35-39 35.8463 37.0 37.0 37.0 37.0 37.0 40-44 35.8429 37.0 37.0 37.0 37.0 37.0 45-49 35.825700000000005 37.0 37.0 37.0 37.0 37.0 50-54 35.6288 37.0 37.0 37.0 37.0 37.0 55-59 35.7029 37.0 37.0 37.0 37.0 37.0 60-64 35.77720000000001 37.0 37.0 37.0 37.0 37.0 65-69 35.6413 37.0 37.0 37.0 37.0 37.0 70-74 35.538 37.0 37.0 37.0 37.0 37.0 75-79 35.4861 37.0 37.0 37.0 37.0 37.0 80-84 35.5134 37.0 37.0 37.0 37.0 37.0 85-89 35.505700000000004 37.0 37.0 37.0 37.0 37.0 90-94 35.495999999999995 37.0 37.0 37.0 37.0 37.0 95-99 35.4859 37.0 37.0 37.0 37.0 37.0 100-104 35.357600000000005 37.0 37.0 37.0 34.6 37.0 105-109 35.3079 37.0 37.0 37.0 32.2 37.0 110-114 35.1258 37.0 37.0 37.0 29.8 37.0 115-119 35.137800000000006 37.0 37.0 37.0 29.8 37.0 120-124 35.1426 37.0 37.0 37.0 32.2 37.0 125-129 35.042500000000004 37.0 37.0 37.0 27.4 37.0 130-134 35.028000000000006 37.0 37.0 37.0 25.0 37.0 135-139 34.8344 37.0 37.0 37.0 25.0 37.0 140-144 34.89640000000001 37.0 37.0 37.0 25.0 37.0 145-149 34.7365 37.0 37.0 37.0 25.0 37.0 150-151 34.4105 37.0 37.0 37.0 25.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.0 1101 2 0.0 1101 3 0.0 1101 4 0.0 1101 5 0.0 1101 6 0.0 1101 7 0.0 1101 8 0.0 1101 9 0.0 1101 10-14 0.0 1101 15-19 0.0 1101 20-24 0.0 1101 25-29 0.0 1101 30-34 0.0 1101 35-39 0.0 1101 40-44 0.0 1101 45-49 0.0 1101 50-54 0.0 1101 55-59 0.0 1101 60-64 0.0 1101 65-69 0.0 1101 70-74 0.0 1101 75-79 0.0 1101 80-84 0.0 1101 85-89 0.0 1101 90-94 0.0 1101 95-99 0.0 1101 100-104 0.0 1101 105-109 0.0 1101 110-114 0.0 1101 115-119 0.0 1101 120-124 0.0 1101 125-129 0.0 1101 130-134 0.0 1101 135-139 0.0 1101 140-144 0.0 1101 145-149 0.0 1101 150-151 0.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 13 3.0 14 4.0 15 4.0 16 3.0 17 6.0 18 1.0 19 5.0 20 3.0 21 8.0 22 9.0 23 13.0 24 13.0 25 10.0 26 8.0 27 12.0 28 14.0 29 23.0 30 40.0 31 50.0 32 60.0 33 106.0 34 261.0 35 811.0 36 2396.0 37 137.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 43.15 21.2 12.049999999999999 23.599999999999998 2 30.45 22.675 29.349999999999998 17.525 3 21.2 26.974999999999998 31.900000000000002 19.925 4 25.75 32.05 23.075000000000003 19.125 5 27.150000000000002 36.075 19.8 16.975 6 21.775 38.35 22.2 17.675 7 22.8 22.575 35.925000000000004 18.7 8 22.875 24.3 27.474999999999998 25.35 9 24.625 24.3 27.975 23.1 10-14 24.615000000000002 27.91 25.96 21.515 15-19 24.21 28.32 26.810000000000002 20.66 20-24 23.919999999999998 28.71 26.66 20.71 25-29 23.415 27.894999999999996 26.325 22.365 30-34 24.255 28.225 26.745 20.775 35-39 24.18 28.87 25.77 21.18 40-44 24.26 28.134999999999998 26.340000000000003 21.265 45-49 23.705000000000002 29.020000000000003 25.974999999999998 21.3 50-54 23.94 27.845 26.955000000000002 21.26 55-59 24.515 28.110000000000003 26.555 20.82 60-64 24.855 28.29 26.215 20.64 65-69 24.555 27.250000000000004 27.355 20.84 70-74 24.42 28.060000000000002 26.805 20.715 75-79 24.875 28.360000000000003 26.540000000000003 20.225 80-84 23.75 28.09 26.919999999999998 21.240000000000002 85-89 25.115 28.000000000000004 26.369999999999997 20.515 90-94 24.709999999999997 27.310000000000002 26.424999999999997 21.555 95-99 25.474999999999998 27.445000000000004 26.284999999999997 20.794999999999998 100-104 25.564999999999998 27.165 27.134999999999998 20.135 105-109 25.16 27.165 26.39 21.285 110-114 24.91 28.4 26.47 20.22 115-119 25.495 28.689999999999998 26.384999999999998 19.43 120-124 25.295 27.284999999999997 26.945000000000004 20.474999999999998 125-129 26.72 27.200000000000003 26.950000000000003 19.13 130-134 26.125 27.384999999999998 26.534999999999997 19.955000000000002 135-139 25.5 28.065 26.245 20.19 140-144 26.36 27.884999999999998 26.0 19.755 145-149 27.105 27.685 25.47 19.74 150-151 28.3625 27.525 26.5875 17.525 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.5 4 1.0 5 0.5 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.5 12 1.0 13 1.0 14 2.0 15 1.5 16 0.5 17 1.0 18 1.0 19 1.0 20 0.5 21 0.5 22 0.5 23 1.0 24 1.5 25 0.5 26 1.5 27 2.0 28 6.0 29 7.0 30 6.5 31 9.0 32 12.0 33 18.5 34 34.0 35 45.5 36 69.5 37 103.0 38 112.0 39 132.0 40 170.0 41 205.5 42 236.5 43 263.5 44 267.0 45 263.5 46 260.5 47 248.5 48 234.5 49 221.0 50 211.5 51 173.5 52 135.0 53 123.5 54 83.5 55 43.0 56 42.5 57 40.0 58 33.0 59 33.0 60 34.0 61 27.5 62 13.5 63 6.5 64 6.5 65 6.0 66 3.0 67 1.0 68 2.0 69 2.0 70 1.0 71 1.0 72 1.0 73 1.0 74 0.5 75 0.5 76 1.0 77 1.5 78 2.5 79 2.0 80 1.5 81 1.0 82 0.5 83 1.0 84 1.0 85 1.0 86 1.5 87 1.5 88 1.0 89 1.0 90 2.0 91 1.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.5 98 1.0 99 1.0 100 3.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150-151 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 59.575 #Duplication Level Percentage of deduplicated Percentage of total 1 63.32354175409149 37.724999999999994 2 20.10071338648762 23.95 3 8.686529584557281 15.525 4 4.280318925723877 10.2 5 1.9723038187159043 5.875 6 0.7553503986571549 2.7 7 0.5035669324381032 2.1 8 0.20981955518254303 1.0 9 0.1258917331095258 0.675 >10 0.0419639110365086 0.25 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source ATTCTTCCAATAGTTTGAAGGCGGCAAATGTTTTCTTGCAGATGTCAGGG 10 0.25 No Hit GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG 9 0.22499999999999998 No Hit TGAAAGTGTCGTAGTTATGTCCGTGTCGGAAGGTGCTGTAGATCATCATG 9 0.22499999999999998 No Hit TGTGTTACTTGTTTTCCATGTTGCTACTCATGCTTCTACCGTGCAAAAAT 9 0.22499999999999998 No Hit ACAACTTGCCCTTCATCAGCCTTTTTTCACAACAGAGCCTCTGACAAGGT 8 0.2 No Hit TCAGACAACCTCAACAATGAAATACCACCCTTCAAAATTTTACTTCCAAT 8 0.2 No Hit GTCTAGAGCAGTGTGGAGCAAAAAGTACTGGTAGCACATTGGATCCAAGA 8 0.2 No Hit GCAACAAGGTAAGATGAGGGTGTTCTCCCGAGGAGATGCCACGGAGATCC 8 0.2 No Hit GGAAGACCCCTCAAACTTTCTACAAAGACCCTGCTTAGGAGCTTCAAGAA 8 0.2 No Hit ACTGACTTGGTTACAGTGCATCCATCATTTGTGATTGGCCCTCTTTTACA 7 0.17500000000000002 No Hit GGGTGGCGGAGCTAATGTTGTGAACCATGGATACACCAAGGGTGATGGCC 7 0.17500000000000002 No Hit CGTCACCGGCCGAACCCCCTGAACAACCCTCGAAAAGCATACTACTGAGC 7 0.17500000000000002 No Hit GGAGTCGACCAGCTTGTGGAGATTATCAAGGTTTTGGGTACTCCAACAAG 7 0.17500000000000002 No Hit CCTGAAGCTGCAGTTTCCCATGAAGGAGATGCCAAGGAGAAGAAGGGACT 7 0.17500000000000002 No Hit GTTTGTGAGCATCCAGAAGCAACAGAAAGTTACTGCACAGTTCCTCAAGC 7 0.17500000000000002 No Hit GAAGAAAAGATCTTGGAAGGATTGCCTAGTTCTGTATCACCTATATTTTC 7 0.17500000000000002 No Hit CGAGCTTCGCTATTTTGAAAAATTAACCCAAAATGGCTTTGCCGAATCAG 7 0.17500000000000002 No Hit TCTTGAAGGTGGTCTTAAGAGTAAGATTGAAATCCAGTGGTCTGATATTA 7 0.17500000000000002 No Hit TGACAAGGCAAGTGGCCTTATAAAGGTTGATCGTGTTCTCTACTCATCAG 7 0.17500000000000002 No Hit TCTGCAACAGTTGCATACTGAACATGAAAAATTGATTGCTGGGTCTCAAA 7 0.17500000000000002 No Hit AGATAAGCTAGGCAAGATGGTTTTACTAGACAAGATGTGGGATGATGTTG 7 0.17500000000000002 No Hit GGGGAAGAAGGCTGTAGCGGTGAAGCTCAAGAGTGTGAAGAGAAAAGCAG 6 0.15 No Hit GACCGCCATTCCCTAAACATTAGTCGCACAGGGTGCGCTGGCCCTGCCAG 6 0.15 No Hit TGTGGTTTTGGTGGTTGATGGAACCAGGATTTGAGGAACACGAACACGTT 6 0.15 No Hit CTGCAATCATCTTCAACAAGGACAGCGCCTGGGATGATCACTGGATTTTC 6 0.15 No Hit GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGT 6 0.15 No Hit AAATTGTTGATCTAAGCTACCTCTCTTCCGTTATCCTCTTAAATCTTCTT 6 0.15 No Hit AATAGAGACTTGGTCCTGGCAGTAGTTAGGAAGGCCAAGAAATCAGCAGC 6 0.15 No Hit CTCTACGAACTTCCATACCTACTTCTTACCAAGGACACAAAGAGATTAAT 6 0.15 No Hit CTTTTCATAGTCCAGTGAGATTGATATTTGGATGGGATATGGTTATGATG 6 0.15 No Hit CCGGATGTTCTTACTTATAATGTTATTTTGTATGCAAAATGTAGGTTGGG 6 0.15 No Hit GAATGAAGAGTGCAGGATCTCTGTAGTTGATCCAGAAAAAAATGTCTTCG 6 0.15 No Hit GCAAGATGGTTTTACTAGACAAGATGTGGGATGATGTTGTTGCTGGACCT 6 0.15 No Hit GGCTCATCTTTGTGGTTGATAGCAATGATCGTGACCGTGTGGTTGAAGCT 6 0.15 No Hit TTCTGACAACTCAGAGGATTCTGACCAGGGTTACATACCATCAGATGCAG 6 0.15 No Hit ATGGATCTTCAAGGTTCACTCCCCCTCCAGGCTTCCTTTGCCGGCCCGAT 6 0.15 No Hit TTTCAATTGAGATGGCAAAGAGGAACTTGAGAGGCATATGGAACTTCACA 6 0.15 No Hit TGGACATGGAGACTGATCGAGCAAGTTTGGAGCAACTTATCAAGAAAAAT 6 0.15 No Hit GGATAACATCTCCGAAGAATTTGCCCTAGTTGCCAAGAAAGGAGCTCCAG 6 0.15 No Hit ATTACAAAGAAGGTGCTACCGATCCAACATTCTTGTACTTTGCCCCTTCT 5 0.125 No Hit CGGGGGGGCTTGAACAAGTTGCTTCAGCAGGAAAAAACGGAGAGGCAGTG 5 0.125 No Hit GAAATATTGTCTGGGACACTAATGTGGCAGCTTATGCAAGTGACTATGTT 5 0.125 No Hit ATTTGCCTTCTTGGGTTGATGGGCCTGCCCGAGACCTTAGAGCTATTGGG 5 0.125 No Hit GAAAGCTAGAGAAGCTAAAAGGCTACAGGAGCAAAACGTTGAACAACAAA 5 0.125 No Hit TGGATGAAAGCAAAAGGGCTGTGCCCACTTCCAAGGGCCAAGCTCTTGCT 5 0.125 No Hit GAAATTAAAACCTACTGCCTTGTGTGTTTTTTCTCCTTTTGCAGGTGACA 5 0.125 No Hit TTTTTTTTTGGGTTTTGGTTCATTGCCTGCAATCTAATAATATTGAAAAG 5 0.125 No Hit TGGGAACGGCGCTCTTTCAAGTGGCACTGCACTCAATCCAACAACAGGTG 5 0.125 No Hit AGCCAGTCCTTCCATGTTCCCAAACATGCTTCCAAGCATATCCAAGGAGT 5 0.125 No Hit CCTAATTTCTATCGTTTAACAGAACCAGCAACCATGGCAGAGCGCCCCCC 5 0.125 No Hit GGACTTTGATTTGCAAAATGAGAGAGAATGTTTCTCGTGCTTCTATGACT 5 0.125 No Hit CTCTCTCTCTCTCATACGGTCTCTCTCTCTCTCTCTCTCGATCAGACCGA 5 0.125 No Hit AGCCTTTTCTGATCCGCCAATCCCCTTAAACATGGCATGCCACAGGAATG 5 0.125 No Hit CTTGATCTGCGTCCCAAGAAGACCAGAGCTATCCGCCGGAGGCTTACCAA 5 0.125 No Hit AAACGCTTCACGGAAATTACAGATGGGACCGTCCTCAACGTCGCTAAAAC 5 0.125 No Hit TTGAGCAAAAGGTGCCAAAAGGGAACAGGAGAAGATTGGCCCAAGATCGC 5 0.125 No Hit CAACAGTACTCTCAGAACCGAAAGATGTGTTACAGGGAGAGATGCTCAAC 5 0.125 No Hit GGCAATGGTTTCGCACCAGCTTATCAGCAGGCTTACTACCGATAGACATG 5 0.125 No Hit CTTTGTTCTGGAGACCAAAAACATCTGGTAGAGGCACAAGAGCATGGAGT 5 0.125 No Hit CAAGTCTTCCATCCCTATTATAGTTTGTCGTAAACGCAGTTCAATATCGG 5 0.125 No Hit GGGTTATGTGAGTGATGAAGTGATGGGCATTTTTGCCCCAATTATCATTT 5 0.125 No Hit ATTACTCCGTGCCATTGCTACACATTGTTTCCCTGCCTTGATTCGTTTGT 5 0.125 No Hit AAAAAACTCTCTGTCTGTCTCTCTCCCCCTCCTCTCTGACTGGCTTGTCT 5 0.125 No Hit CTCCACAGGAACTTTGGCCACCGAAACAATATCCTTTAGCCATTTAAAGT 5 0.125 No Hit GCTGGATGACGGGTCTTCTAAGTTTTGCAGGGACAATTCGCTACATGTAT 5 0.125 No Hit GCGCTTATCCCTGCCTATCCGGAGATCCAAGCCAAGGCTCATGAGGAACT 5 0.125 No Hit AGCTCTCTTCTCCAACTTCTAGTACTGTGGTTTCAAGTAAAGTAAAAGAA 5 0.125 No Hit AGGAGATGAGGAGGAAGCAACAACAACAGGCCCAAGAAGCATATGATGAA 5 0.125 No Hit GTGAACTTGAGTCCACTCTGCTGTCACAGCAAGGATTTCCATTTGCCCAT 5 0.125 No Hit GTACGAGTTCCAAATGCAATATGGATCAATTGGTTGGTCAGTTGGTGCGA 5 0.125 No Hit GGTGGATCTGGATCTACAAAGCAGGAGTTTCTGGATGCCATTTCCTATGG 5 0.125 No Hit AGAAAAGGTTGCGGACTTTGAGAAATCAGATCCTTGTGCAGTGATGGATC 5 0.125 No Hit CTCCAAGGCAAGGTATGATGAAATTGTCAAGGAAGTGTCCTCCTACTTGA 5 0.125 No Hit AGACTCTCATCAGAAAAGCTTAGACTCATCCGATTTAGGGTTTCGCTTCA 5 0.125 No Hit TTGCTCACAAGTTGTGGACTCAGTCTCGTAGGCCATTGGCGTTGGCTTTA 5 0.125 No Hit TGAAAAGAAGAAGGTGATGGTGGCAATAGATGATAGTGAGAGCAGCCACT 5 0.125 No Hit CCCCACAAGGATTTATAGTGGGTCATGGGACATGACTGTTCGTATATGGG 5 0.125 No Hit GGGAAAATTAACCTAGATGAATACGTGGAGTATCTCAAGAAAGATAACAA 5 0.125 No Hit ATTGCTTCCTCCTCTGTTGCTACTGAATGGGTGAAAGGGAAGCAAATGGA 5 0.125 No Hit AGGAGTCCACCCTCCACTTGGTGCTTCGTCTACGTGGTGGCATGCAGATT 5 0.125 No Hit CCTCCGCCTCCGCCCTATCTTTGGGTGTAAGAAGAAAAAGAAATAAAAAG 5 0.125 No Hit GCTGAAACTGAGGCTCCAGTCGTGGAGCAACCAACTGCTACGGAGGAGCC 5 0.125 No Hit TCAAGGAATGGGAAGCAATATTTGCAATTGGTCTACGTTATCGGTCCACC 5 0.125 No Hit GTTTTTTGGCGATTATTGTTTGATTTTAGTGTTGGAGAAATGCTATAGAC 5 0.125 No Hit GCGTGTGATACAAGCGGTTCGCAAGAAATTAAGTCCCTACTGGCCTGCAC 5 0.125 No Hit TGGCAGAATTTCCTCCCACTCCACCTCAAGTTTTAGTTGATAAGTTCAGA 5 0.125 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.025 0.0 0.0 0.0 0.0 62-63 0.025 0.0 0.0 0.0 0.0 64-65 0.025 0.0 0.0 0.0 0.0 66-67 0.0625 0.0 0.0 0.0 0.0 68-69 0.1 0.0 0.0 0.0 0.0 70-71 0.1 0.0 0.0 0.0 0.0 72-73 0.1 0.0 0.0 0.0 0.0 74-75 0.1125 0.0 0.0 0.0 0.0 76-77 0.175 0.0 0.0 0.0 0.0 78-79 0.2375 0.0 0.0 0.0 0.0 80-81 0.275 0.0 0.0 0.0 0.0 82-83 0.275 0.0 0.0 0.0 0.0 84-85 0.325 0.0 0.0 0.0 0.0 86-87 0.4375 0.0 0.0 0.0 0.0 88-89 0.575 0.0 0.0 0.0 0.0 90-91 0.725 0.0 0.0 0.0 0.0 92-93 0.95 0.0 0.0 0.0 0.0 94-95 1.15 0.0 0.0 0.0 0.0 96-97 1.2 0.0 0.0 0.0 0.0 98-99 1.3875 0.0 0.0 0.0 0.0 100-101 1.55 0.0 0.0 0.0 0.0 102-103 1.6625 0.0 0.0 0.0 0.0 104-105 1.9375 0.0 0.0 0.0 0.0 106-107 2.2375 0.0 0.0 0.0 0.0 108-109 2.6 0.0 0.0 0.0 0.0 110-111 3.0250000000000004 0.0 0.0 0.0 0.0 112-113 3.5374999999999996 0.0 0.0 0.0 0.0 114-115 3.9625000000000004 0.0 0.0 0.0 0.0 116-117 4.25 0.0 0.0 0.0 0.0 118-119 4.5375 0.0 0.0 0.0 0.0 120-121 5.0875 0.0 0.0 0.0 0.0 122-123 5.7875 0.0 0.0 0.0 0.0 124-125 6.199999999999999 0.0 0.0 0.0 0.0 126-127 6.5375 0.0 0.0 0.0 0.0 128-129 6.800000000000001 0.0 0.0 0.0 0.0 130-131 7.3 0.0 0.0 0.0 0.0 132-133 7.824999999999999 0.0 0.0 0.0 0.0 134-135 8.4375 0.0 0.0 0.0 0.0 136-137 9.4875 0.0 0.0 0.0 0.0 138-139 10.3125 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ACACGAG 10 0.006830828 145.0 4 GTTTGCC 10 0.006830828 145.0 6 CACGAGG 10 0.006830828 145.0 5 GAACACG 10 0.006830828 145.0 2 TGAACAT 10 0.006830828 145.0 145 AACACGA 10 0.006830828 145.0 3 >>END_MODULE Read 985370 spots for SRR26075388.sra Written 985370 spots for SRR26075388.sra Read 985370 spots for SRR26075388.sra Written 985370 spots for SRR26075388.sra Read 985370 spots for SRR26075388.sra Written 985370 spots for SRR26075388.sra Read 985370 spots for SRR26075388.sra Written 985370 spots for SRR26075388.sra Read 985370 spots for SRR26075388.sra Written 985370 spots for SRR26075388.sra Read 985370 spots for SRR26075388.sra Written 985370 spots for SRR26075388.sra Read 985370 spots for SRR26075388.sra Written 985370 spots for SRR26075388.sra Read 985389 spots for SRR26075388.sra Written 985389 spots for SRR26075388.sra Read 985370 spots for SRR26075388.sra Written 985370 spots for SRR26075388.sra Read 985370 spots for SRR26075388.sra Written 985370 spots for SRR26075388.sra Read 985370 spots for SRR26075388.sra Written 985370 spots for SRR26075388.sra Read 985370 spots for SRR26075388.sra Written 985370 spots for SRR26075388.sra Read 985370 spots for SRR26075388.sra Written 985370 spots for SRR26075388.sra Read 985370 spots for SRR26075388.sra Written 985370 spots for SRR26075388.sra Read 985370 spots for SRR26075388.sra Written 985370 spots for SRR26075388.sra Read 985370 spots for SRR26075388.sra Written 985370 spots for SRR26075388.sra Read 985370 spots for SRR26075388.sra Written 985370 spots for SRR26075388.sra Read 985370 spots for SRR26075388.sra Written 985370 spots for SRR26075388.sra Read 985370 spots for SRR26075388.sra Written 985370 spots for SRR26075388.sra Read 985370 spots for SRR26075388.sra Written 985370 spots for SRR26075388.sra SRR ids: ['SRR26075388.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_pca0hmi2 SRR26075388.sra spots: 19707419 blocks: [[1, 985370], [985371, 1970740], [1970741, 2956110], [2956111, 3941480], [3941481, 4926850], [4926851, 5912220], [5912221, 6897590], [6897591, 7882960], [7882961, 8868330], [8868331, 9853700], [9853701, 10839070], [10839071, 11824440], [11824441, 12809810], [12809811, 13795180], [13795181, 14780550], [14780551, 15765920], [15765921, 16751290], [16751291, 17736660], [17736661, 18722030], [18722031, 19707419]] SRR26075388 file size 7272923 SRR26075388 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o SRR26075388 SRR26075388_1.fastq SRR26075388_2.fastq Input file: SRR26075388_1.fastq Paired file: SRR26075388_2.fastq trimmed: SRR26075388-trimmed-pair1.fastq, SRR26075388-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Wed Feb 12 04:16:26 2025 >> started Wed Feb 12 04:16:50 2025 >> done (23.374s) 19707419 read pairs processed; of these: 55 ( 0.00%) short read pairs filtered out after trimming by size control 37444 ( 0.19%) empty read pairs filtered out after trimming by size control 19669920 (99.81%) read pairs available; of these: 2986148 (15.18%) trimmed read pairs available after processing 16683772 (84.82%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 18 4 0.00% 19 9 0.00% 20 14 0.00% 21 8 0.00% 22 13 0.00% 23 21 0.00% 24 11 0.00% 25 9 0.00% 26 13 0.00% 27 15 0.00% 28 21 0.00% 29 20 0.00% 30 31 0.00% 31 20 0.00% 32 25 0.00% 33 31 0.00% 34 30 0.00% 35 18 0.00% 36 17 0.00% 37 30 0.00% 38 26 0.00% 39 33 0.00% 40 41 0.00% 41 51 0.00% 42 48 0.00% 43 50 0.00% 44 59 0.00% 45 57 0.00% 46 45 0.00% 47 77 0.00% 48 41 0.00% 49 101 0.00% 50 99 0.00% 51 77 0.00% 52 116 0.00% 53 117 0.00% 54 149 0.00% 55 138 0.00% 56 192 0.00% 57 167 0.00% 58 226 0.00% 59 245 0.00% 60 285 0.00% 61 348 0.00% 62 421 0.00% 63 442 0.00% 64 554 0.00% 65 537 0.00% 66 615 0.00% 67 714 0.00% 68 756 0.00% 69 965 0.00% 70 1137 0.01% 71 1218 0.01% 72 1524 0.01% 73 1686 0.01% 74 1883 0.01% 75 2021 0.01% 76 2562 0.01% 77 2776 0.01% 78 2831 0.01% 79 3259 0.02% 80 3721 0.02% 81 4032 0.02% 82 4690 0.02% 83 5263 0.03% 84 6035 0.03% 85 6790 0.03% 86 7336 0.04% 87 7876 0.04% 88 8572 0.04% 89 9358 0.05% 90 9966 0.05% 91 11207 0.06% 92 11993 0.06% 93 13395 0.07% 94 14596 0.07% 95 15514 0.08% 96 16411 0.08% 97 17777 0.09% 98 18156 0.09% 99 18789 0.10% 100 20228 0.10% 101 20911 0.11% 102 22608 0.11% 103 24199 0.12% 104 25644 0.13% 105 26899 0.14% 106 28561 0.15% 107 29645 0.15% 108 30614 0.16% 109 31285 0.16% 110 32378 0.16% 111 33721 0.17% 112 35126 0.18% 113 35780 0.18% 114 38552 0.20% 115 40476 0.21% 116 42076 0.21% 117 43227 0.22% 118 45522 0.23% 119 45840 0.23% 120 46845 0.24% 121 48027 0.24% 122 47829 0.24% 123 50440 0.26% 124 52210 0.27% 125 54884 0.28% 126 55923 0.28% 127 57726 0.29% 128 59731 0.30% 129 59910 0.30% 130 61296 0.31% 131 61552 0.31% 132 63454 0.32% 133 64280 0.33% 134 65699 0.33% 135 67891 0.35% 136 70307 0.36% 137 71194 0.36% 138 72027 0.37% 139 73780 0.38% 140 75458 0.38% 141 75661 0.38% 142 75891 0.39% 143 76747 0.39% 144 79303 0.40% 145 81221 0.41% 146 81581 0.41% 147 82876 0.42% 148 84525 0.43% 149 86851 0.44% 150 87211 0.44% 151 16683772 84.82% 19669920 reads passed initial QC criterion=sequence-density sequence-density=0.39 sequence-density-rank=1 fanout-score=2.98 fanout-score-rank=23 prefix-density=0.55 prefix-fanout=2.1 sequence=CACTTGCAGCCATTCTCAGCACCA criterion=fanout-score sequence-density=0.04 sequence-density-rank=36 fanout-score=87.28 fanout-score-rank=1 prefix-density=0.36 prefix-fanout=8.5 sequence=CATCACCATCCCTGATTGATCTTTGATTCACAACAAGACCAACCAACCGTACAATGTTTACATAACTTGGGATAATCTCACAAGAAAGAAAGATCAATACAAAACTAGTCAGCTCAGGATGTTCCAGGCACGGTAGAACCGTGGAACCATAACAACAAGAGACATATTGCAGATAAGTACTGAAAAACAAAACACAGTACGTATTTACATGGGCAACCTTGGTTGAAGGCAACCTCATCAACGATGCTCGCTCTTCGTCTCTCCACTGTACATCCAGTCATAGACAGTGGGTGTGTTAGGCTGTGGCTTGTCAAAAACATGAGCACCAATATTCTTAGTAGCAAGGTTGCTACCAGGGTGGAACACGCTCCTCCAAACATTGCTACGCGCCGACACTGGGGTTGTAGGGGTCACTGGTGTCGTCGGTGTCCCTGGAGTTCCTGGCATAGTCATGGACCTCTGAAACTTATTAACAGGACTGCTCCCCTCTCCGACGTCAATATCTTTGATG criterion=sequence-density sequence-density=0.46 sequence-density-rank=1 fanout-score=2.90 fanout-score-rank=24 prefix-density=0.47 prefix-fanout=2.9 sequence=ATGTACCCTGACTT criterion=fanout-score sequence-density=0.01 sequence-density-rank=31 fanout-score=191.92 fanout-score-rank=1 prefix-density=0.13 prefix-fanout=8.7 sequence=AGAGAAAAGATAAGCTAGGCAAGATGGTTTTACTAGACAAGATGTGGGATGATGTTGTTGCTGGACCTCAGCCAGAACGTGGCCTTGG SRR26075388 testing PE reads STAR mapping to Ensembl genome Started job on | Feb 12 04:17:39 Started mapping on | Feb 12 04:17:39 Finished on | Feb 12 04:23:16 Mapping speed, Million of reads per hour | 210.12 Number of input reads | 19669920 Average input read length | 294 UNIQUE READS: Uniquely mapped reads number | 16138014 Uniquely mapped reads % | 82.04% Average mapped length | 293.22 Number of splices: Total | 14695524 Number of splices: Annotated (sjdb) | 14313427 Number of splices: GT/AG | 14419620 Number of splices: GC/AG | 208624 Number of splices: AT/AC | 14839 Number of splices: Non-canonical | 52441 Mismatch rate per base, % | 0.42% Deletion rate per base | 0.03% Deletion average length | 2.97 Insertion rate per base | 0.02% Insertion average length | 2.58 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 598268 % of reads mapped to multiple loci | 3.04% Number of reads mapped to too many loci | 90722 % of reads mapped to too many loci | 0.46% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 14.15% % of reads unmapped: other | 0.30% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 2933638 2933638 2933638 N_multimapping 598268 598268 598268 N_noFeature 464622 15929172 571933 N_ambiguous 191873 973 89748 UnstrandedReadsAssigned:15481519 PositiveStrandReadsAssigned:207869 NegativeStrandReadsAssigned:15476333 Dataset is classified negative stranded MeadianReadLen=151 20thPercentileLength=151 echo kmer=147 SRR26075388 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,400 [index] number of k-mers: 62,057,036 [index] number of equivalence classes: 130,681 [quant] running in paired-end mode [quant] will process pair 1: SRR26075388-trimmed-pair1.fastq SRR26075388-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 19,669,920 reads, 15,782,820 reads pseudoaligned [quant] estimated average fragment length: 214.473 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,207 rounds 52401 SRR26075388.ke.tsv 34699 SRR26075388.se.tsv 87100 total ==> SRR26075388.ke.tsv <== target_id length eff_length est_counts tpm Potri.005G200100.1.v4.1 2018 1804.53 1526 45.6421 Potri.005G024800.1.v4.1 1035 821.527 816 53.6096 Potri.004G059700.1.v4.1 961 747.527 7 0.505412 Potri.007G009000.2.v4.1 1416 1202.53 0 0 Potri.003G141000.2.v4.1 2943 2729.53 703 13.9009 Potri.016G087400.1.v4.1 270 88.4395 1520 927.623 Potri.015G069301.1.v4.1 564 352.377 0 0 Potri.010G195200.1.v4.1 1773 1559.53 274 9.48269 Potri.012G127500.1.v4.1 977 763.527 26661 1884.63 ==> SRR26075388.se.tsv <== Potri.001G166300.v4.1 0 Potri.001G448400.v4.1 151 Potri.001G233950.v4.1 0 Potri.001G122700.v4.1 391 Potri.001G212900.v4.1 0 Potri.001G182400.v4.1 0 Potri.001G256600.v4.1 0 Potri.001G040500.v4.1 2 Potri.001G416900.v4.1 0 Potri.001G452600.v4.1 658 SRR26075388 completed mapping pipeline successfully