Starting /dee2/code/volunteer_pipeline.sh SRR26075389 current disk space = 3051724492800 free memory = 1490525036 SRR26075389 SRAfilesize 8269ace60d048a32baaf749b1b2e143d SRR26075389.sra SRR26075389.sra file validated SRR26075389 is paired end SRR26075389 is conventional basespace SRR26075389 read1 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR26075389_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.656 37.0 37.0 37.0 37.0 37.0 2 36.594 37.0 37.0 37.0 37.0 37.0 3 36.513 37.0 37.0 37.0 37.0 37.0 4 36.703 37.0 37.0 37.0 37.0 37.0 5 36.732 37.0 37.0 37.0 37.0 37.0 6 36.6665 37.0 37.0 37.0 37.0 37.0 7 36.6345 37.0 37.0 37.0 37.0 37.0 8 36.6415 37.0 37.0 37.0 37.0 37.0 9 36.6285 37.0 37.0 37.0 37.0 37.0 10-14 36.650800000000004 37.0 37.0 37.0 37.0 37.0 15-19 36.61800000000001 37.0 37.0 37.0 37.0 37.0 20-24 36.5388 37.0 37.0 37.0 37.0 37.0 25-29 36.4331 37.0 37.0 37.0 37.0 37.0 30-34 36.4071 37.0 37.0 37.0 37.0 37.0 35-39 36.348 37.0 37.0 37.0 37.0 37.0 40-44 36.312 37.0 37.0 37.0 37.0 37.0 45-49 36.1357 37.0 37.0 37.0 37.0 37.0 50-54 36.056799999999996 37.0 37.0 37.0 37.0 37.0 55-59 36.0037 37.0 37.0 37.0 37.0 37.0 60-64 35.9174 37.0 37.0 37.0 37.0 37.0 65-69 35.83149999999999 37.0 37.0 37.0 37.0 37.0 70-74 35.9335 37.0 37.0 37.0 37.0 37.0 75-79 35.9784 37.0 37.0 37.0 37.0 37.0 80-84 35.9803 37.0 37.0 37.0 37.0 37.0 85-89 35.848 37.0 37.0 37.0 37.0 37.0 90-94 35.802099999999996 37.0 37.0 37.0 37.0 37.0 95-99 35.87350000000001 37.0 37.0 37.0 37.0 37.0 100-104 35.81419999999999 37.0 37.0 37.0 37.0 37.0 105-109 35.775 37.0 37.0 37.0 37.0 37.0 110-114 35.6049 37.0 37.0 37.0 37.0 37.0 115-119 35.469800000000006 37.0 37.0 37.0 37.0 37.0 120-124 35.5236 37.0 37.0 37.0 37.0 37.0 125-129 35.344500000000004 37.0 37.0 37.0 34.6 37.0 130-134 35.2236 37.0 37.0 37.0 32.2 37.0 135-139 35.1551 37.0 37.0 37.0 29.8 37.0 140-144 34.9037 37.0 37.0 37.0 25.0 37.0 145-149 34.9528 37.0 37.0 37.0 25.0 37.0 150-151 34.759 37.0 37.0 37.0 25.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.0 1101 2 0.0 1101 3 0.0 1101 4 0.0 1101 5 0.0 1101 6 0.0 1101 7 0.0 1101 8 0.0 1101 9 0.0 1101 10-14 0.0 1101 15-19 0.0 1101 20-24 0.0 1101 25-29 0.0 1101 30-34 0.0 1101 35-39 0.0 1101 40-44 0.0 1101 45-49 0.0 1101 50-54 0.0 1101 55-59 0.0 1101 60-64 0.0 1101 65-69 0.0 1101 70-74 0.0 1101 75-79 0.0 1101 80-84 0.0 1101 85-89 0.0 1101 90-94 0.0 1101 95-99 0.0 1101 100-104 0.0 1101 105-109 0.0 1101 110-114 0.0 1101 115-119 0.0 1101 120-124 0.0 1101 125-129 0.0 1101 130-134 0.0 1101 135-139 0.0 1101 140-144 0.0 1101 145-149 0.0 1101 150-151 0.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 20 3.0 21 3.0 22 3.0 23 5.0 24 9.0 25 8.0 26 15.0 27 12.0 28 15.0 29 22.0 30 29.0 31 48.0 32 103.0 33 125.0 34 158.0 35 402.0 36 2848.0 37 192.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 43.568568568568566 14.98998998998999 6.731731731731731 34.709709709709706 2 19.475 15.35 33.625 31.55 3 18.45 17.875 28.349999999999998 35.325 4 21.475 24.099999999999998 26.150000000000002 28.275 5 24.55 27.900000000000002 26.275 21.275 6 23.225 33.725 21.349999999999998 21.7 7 16.575 29.099999999999998 39.35 14.975 8 18.35 25.724999999999998 31.125000000000004 24.8 9 18.025 24.075 34.849999999999994 23.05 10-14 20.785 28.99 26.915 23.31 15-19 20.794999999999998 26.43 28.194999999999997 24.58 20-24 20.985 27.150000000000002 27.505000000000003 24.36 25-29 19.955000000000002 27.04 29.315 23.69 30-34 20.205000000000002 27.82 27.865000000000002 24.11 35-39 20.979999999999997 27.595 27.805000000000003 23.62 40-44 21.25 26.875 28.415000000000003 23.46 45-49 21.740000000000002 26.705000000000002 27.435 24.12 50-54 21.58 26.82 28.29 23.31 55-59 21.9 27.025 27.950000000000003 23.125 60-64 21.875 26.765 27.500000000000004 23.86 65-69 20.71 27.474999999999998 27.965 23.849999999999998 70-74 21.825 27.1 27.58 23.494999999999997 75-79 21.759999999999998 26.415 27.71 24.115000000000002 80-84 21.715 26.96 27.515 23.810000000000002 85-89 21.665 27.57 26.584999999999997 24.18 90-94 21.105 27.48 27.415 24.0 95-99 22.06 26.784999999999997 28.050000000000004 23.105 100-104 21.8 27.58 26.57 24.05 105-109 22.17 26.545 27.725 23.56 110-114 21.790000000000003 27.065 26.985 24.16 115-119 22.375 26.645000000000003 27.450000000000003 23.53 120-124 22.45 27.18 27.065 23.305 125-129 22.384999999999998 27.575 26.369999999999997 23.669999999999998 130-134 22.11 27.02 25.990000000000002 24.88 135-139 22.795 27.345000000000002 26.3 23.56 140-144 22.775000000000002 27.250000000000004 25.72 24.255 145-149 23.07 28.035 24.715 24.18 150-151 22.412499999999998 26.9625 25.3 25.324999999999996 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.5 13 1.0 14 1.0 15 1.5 16 1.0 17 0.0 18 0.0 19 0.0 20 0.5 21 1.5 22 1.5 23 1.5 24 1.0 25 2.0 26 3.0 27 2.5 28 3.5 29 9.0 30 14.0 31 15.5 32 17.0 33 20.5 34 33.5 35 54.5 36 75.5 37 82.5 38 106.5 39 157.5 40 189.5 41 189.5 42 209.5 43 271.5 44 281.5 45 267.0 46 266.5 47 242.0 48 241.5 49 219.5 50 170.5 51 143.5 52 113.0 53 106.0 54 104.5 55 76.0 56 61.5 57 53.5 58 36.0 59 25.5 60 21.5 61 12.0 62 8.0 63 8.5 64 4.0 65 3.0 66 4.5 67 4.5 68 3.5 69 4.0 70 5.5 71 6.0 72 11.0 73 13.0 74 5.0 75 2.5 76 3.0 77 2.5 78 1.5 79 0.5 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.1 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150-151 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 59.025000000000006 #Duplication Level Percentage of deduplicated Percentage of total 1 60.779330792037264 35.875 2 23.08343922066921 27.250000000000004 3 8.640406607369759 15.299999999999999 4 4.320203303684879 10.2 5 1.736552308343922 5.125 6 0.6776789495976281 2.4 7 0.29648454044896233 1.225 8 0.25412960609911056 1.2 9 0.042354934349851756 0.22499999999999998 >10 0.16941973739940702 1.2 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCAAGCAAATCTCGGTT 17 0.42500000000000004 TruSeq Adapter, Index 2 (97% over 36bp) GGAGGCGGCTCAGACAGCCAACTCTGTTCGAGAAATCAGGATTCCATCAG 11 0.27499999999999997 No Hit ATCAATTCTGATTTCGTGAAATGCTCTGTAACAGAGTACTGATTTGAATG 10 0.25 No Hit ACTCTTTCTTCGCTTCCTTGGTAGTTTCCTTCACCTTTGGTCTTCCCTGT 10 0.25 No Hit GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCAAGCAAATCTCGGGT 9 0.22499999999999998 TruSeq Adapter, Index 2 (97% over 36bp) AAAGAGAGACAGAGAGAGAGAGAGGGAGGGAGAAAGAGAAATTTCTCTGA 8 0.2 No Hit CCATAATGTAACCCATGCTGTAAACCAGCCAGATGAAGATTAGAGTTTGA 8 0.2 No Hit TGCTTTAGTGGATGTTGCTGCAGGTGTTTTAATGGGTGGTGGTGATTTGG 8 0.2 No Hit CACCAGGCCACTTCCCCGCGGTGTACTTAAAAGTACCAGCTTTGCCTTCA 8 0.2 No Hit CCTCGAGCTTACCGGATTGGCCTCTTTGGGGCTCCGAATTGGCAACCTCG 8 0.2 No Hit AAGCTTGCCAAAGTGAAAGCTCAGGTCTCTGAAGTTAAGGGTGTCATGAT 8 0.2 No Hit CACATGTCAATCAGGCTTCTCAACTCATCCTGCTTACTTGATGGAGCTTT 7 0.17500000000000002 No Hit GACACGCGCTTCCCCGTATTCATTGAAATTGCCGCTCTGTCCGTCTGATC 7 0.17500000000000002 No Hit CTTGGCACAAACACCCGGCGTACTCCCTTTAGCAATAGTATTCTCAAAAA 7 0.17500000000000002 No Hit CGGGCTGTCCTCCTTGCGGTCTCGAATCCATTGGTTTCGCTCGATTTCTT 7 0.17500000000000002 No Hit GGAGGATCAGGTAGAGAAGAAGGGAAAGCTATTTCTCTTAAATCGTAGCG 7 0.17500000000000002 No Hit CCAGCCTCTTCATCTTGAATCTTGAAAAACAAACCTTGAATATTTACAAT 7 0.17500000000000002 No Hit ATGACAAGTATACAAGTGGAGAGAGCCAAAGTGTTTGATACCAAGAAAAC 7 0.17500000000000002 No Hit CCAGATTCTTCTATAAAAATGAAAGAGTATTGTTCTAAAATCGTATGGTA 6 0.15 No Hit GTTTCAACTCCTGATCCCGCAGCCAGTTCCAGAGACCTGCCTTGCTTGAA 6 0.15 No Hit GGGTTTCTTGCTTGGACTTGGAGGGTTTGGGCGTGCTTGTCTCATCTTCT 6 0.15 No Hit GTCAACCTGCGAATCTCGACCGACAGCATCAAATTACCATCTTGCCCTTC 6 0.15 No Hit CCGGTCTTTGTCCAGTTTGAAACATCAGAATCTACAATGTTAGCAGTCGC 6 0.15 No Hit GCTCTCTTGGTTTTGTTTGTAACTTTTCTTTGCGTTCTTGCTTGTATCCA 6 0.15 No Hit CCACGAGGTCGCTGGGTGCTCTGGTCGTACATTATTACTACATCAGTTAC 6 0.15 No Hit GTCCAATGCTGATCTTATTGTCATCTTTTGTTGTTGTAGCTCGAGATGCC 6 0.15 No Hit CCCCACTTGGCCGCTTCATCTGCATCATACTTTTCTTCAGATTTTGCCGT 6 0.15 No Hit AGACTGTAGTAGGATCTGTTATCCCTGATTACTATCTCTGCAAAGTGCAG 6 0.15 No Hit ACTGTATTGCATCTTCGCGCTTTCAGTTGCCGAGAAGTAAGACCCACCTT 6 0.15 No Hit CCGTCCTCTTCAGGGAATCAGGAAAACAAAATCAAGCAGCAACTTCTTCT 6 0.15 No Hit CTTTATCATTTCAAGCCCTTGCTCTAACTTGACCTCTTGAGTGTCCCTGC 6 0.15 No Hit CAAATATATATTTGCAACCCAACTATCACCCAAAGAAGGGCTCTTTTCCC 6 0.15 No Hit TGGCCCTGACCCCGGGGAACAGTCGCATGACGAGGCAGTTTCCAGAAACG 6 0.15 No Hit GTCTCAGGCTGGCGGAACAGGGGCATAATGTCAATGAAGTTGATTCCGGG 6 0.15 No Hit ATTGTGTCCGAGCTCTCAACTTCCAAGGTTATGGTCTTCCCAGTGAGGGT 5 0.125 No Hit GTGCTAGCCCGTCGAGACTGAAAAGCTATAACCCGCAGACCCGAGCGAAA 5 0.125 No Hit TGGCGCAAAAACAGCGGCGGCGGAACCCTTTGCATTCACCACCGGGAAAA 5 0.125 No Hit ATGCCAGTATGCAACCTTAGCCAGATTTGTGGACTTTCCAACCCCATTGA 5 0.125 No Hit ATTTTGGGACGGGGAATGTGAGGCAACTTAACAGAGCCACTGAACCTCTT 5 0.125 No Hit GCATCCTTGAGGGAAAGAAGCAAGTGACTTGCCACAAGCGCCCTTAAGCA 5 0.125 No Hit ACAGCAACACATTCCTGAAGAAGAAACCAGAGCAACCCTAATCGAACTCA 5 0.125 No Hit CTGCATGGTGGTGATCCACAGTAAAATGGTGGTGATGATGCCACCTGGAA 5 0.125 No Hit CTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTT 5 0.125 No Hit AACCTCTCCTCGAACCTTGTCTTTGTTTCCTTACAAGCCACGACTTTCTT 5 0.125 No Hit GCCAGAGTGACGTCCCTTTCTCTTAGCTTCCTTCATGCGTTGAGCGCGGG 5 0.125 No Hit GGCCTGTTGTTGTTGCTTCCTCCTCATCTCCTCCTCAATGTTCACATCAT 5 0.125 No Hit ATCCAACCACACTTCCTCCTACAACCTGCCCTTGCCCACCGGCTAGGTAT 5 0.125 No Hit GTCGTCGGATTTTGTGTTTCGGTCACCGGAGTCCGTAAAAATCAATTCCG 5 0.125 No Hit CTTGTACATATGGGCAATCTCCGGTACAAGAGGATCATCAGGATTTGGAT 5 0.125 No Hit GCCATGTACAATGGGTCGAAGAGGTCCGGCCGTAGGTCGAGGAAAGATTT 5 0.125 No Hit AGCGTTTATTGCCGTTATTGGAGTTGCGATTATCGTTGTTGGAGTTGCGG 5 0.125 No Hit GCTTCTCTTTGAGCTTGTCCACTACAAGACCGTTTGCTGAAGGACTGGGT 5 0.125 No Hit CCTAGGGTTTCTTTCAGAAGGTGCAGGAACTTATGCCTCCACATTTTATC 5 0.125 No Hit AGGCAAGCCAGTTGTATTTGGGTAGCCAATCCAAGTCACCTGAACAGGTG 5 0.125 No Hit GTCCTCATTACGGTCCCTATCCCGCTGGCTAGACCTATCGTGAGAGCGTC 5 0.125 No Hit TTCTTAGTGAGGGTGTTGGGGAAGTTACAAGGGCGTTGTGCTCCACAAGC 5 0.125 No Hit GGAGCATTTAATCTGGGCAGATCTTCCGTGAAAGGTGGATCATGAGCTTC 5 0.125 No Hit CACAGTTTTGTCCACTAATTTTCCATCTTTCAGGAAAATAAAAGTTGGCA 5 0.125 No Hit GTGGTTTCTCAGCAGGGGCAGCGTCTTTAACTTTAGGAGCGTGTTTAGGG 5 0.125 No Hit AGATGGTTTTGGTTTGCATCAAGGGAAATGGTTTCATTGTGCTCATCAGC 5 0.125 No Hit CTGAATTTCATGTATTCCCTGTTCTCTCTCCTCGATAACAGCCTCATTGA 5 0.125 No Hit GCCCTCTCCGGCGCCCCTTTCCAGGGGACTTGGGCCTGGTCCGCCGCTGA 5 0.125 No Hit GTTGTATTTTGCAAGACGTTCTGATCTGCAAGGAGCTCCAGTCTTGATCT 5 0.125 No Hit AGCGGCGGTTGGTGCAGGAGCTGGAGCTGACATAGGAGCAGCGGCGTGAA 5 0.125 No Hit CATAAATCTTTAAGGCCTTCGATTTTGTCTTGACAAAACTGCCTTCATGT 5 0.125 No Hit GCTTGTTTCATTGCAGCGCAGTGCCATGGCCGTCCTTTGTTTAAGTGAAT 5 0.125 No Hit CTCACGAAGTACAAATATATGGCCAGAGCCATCCCACTTTAATCATGGTT 5 0.125 No Hit TGGTAATATGTACTCGCATGTTGCAAGGTAAAGAACTGTGAAAAACGATA 5 0.125 No Hit GGGGAGAAGCATCTCGATTTCTATTTTAATGAAAGGTGATTTCCCCTCAT 5 0.125 No Hit GGGACTGTACCGGTGAGCTTATTGGAGGACAAATCAAGAAGCTGGAGCTT 5 0.125 No Hit GCAAAGGAAGAACAATTAACAACGTGCATCCCTAGTTGTTTTGGTCCAAC 5 0.125 No Hit GGAGACATTGAAAAGCTATGGAATTGAAATTCCCACTGAACTCAGTATAG 5 0.125 No Hit AGTGAATTATAGAAACAGAAAGGCATCATTCATTGGGTAGCAAAACCAAA 5 0.125 No Hit CATCTTTTCTTAAAACCATATTTCTTGCGTTCATAGAACAGATCCGTCTT 5 0.125 No Hit GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCAAGCAAATCGCGGTT 5 0.125 TruSeq Adapter, Index 2 (97% over 36bp) >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0125 0.0 0.0 0.0 0.0 50-51 0.025 0.0 0.0 0.0 0.0 52-53 0.025 0.0 0.0 0.0 0.0 54-55 0.025 0.0 0.0 0.0 0.0 56-57 0.025 0.0 0.0 0.0 0.0 58-59 0.05 0.0 0.0 0.0 0.0 60-61 0.05 0.0 0.0 0.0 0.0 62-63 0.05 0.0 0.0 0.0 0.0 64-65 0.0625 0.0 0.0 0.0 0.0 66-67 0.075 0.0 0.0 0.0 0.0 68-69 0.075 0.0 0.0 0.0 0.0 70-71 0.075 0.0 0.0 0.0 0.0 72-73 0.075 0.0 0.0 0.0 0.0 74-75 0.15 0.0 0.0 0.0 0.0 76-77 0.23750000000000002 0.0 0.0 0.0 0.0 78-79 0.3 0.0 0.0 0.0 0.0 80-81 0.3 0.0 0.0 0.0 0.0 82-83 0.3125 0.0 0.0 0.0 0.0 84-85 0.325 0.0 0.0 0.0 0.0 86-87 0.38749999999999996 0.0 0.0 0.0 0.0 88-89 0.5375 0.0 0.0 0.0 0.0 90-91 0.675 0.0 0.0 0.0 0.0 92-93 0.775 0.0 0.0 0.0 0.0 94-95 0.825 0.0 0.0 0.0 0.0 96-97 0.925 0.0 0.0 0.0 0.0 98-99 1.0499999999999998 0.0 0.0 0.0 0.0 100-101 1.1125 0.0 0.0 0.0 0.0 102-103 1.4125 0.0 0.0 0.0 0.0 104-105 1.875 0.0 0.0 0.0 0.0 106-107 2.325 0.0 0.0 0.0 0.0 108-109 2.6375 0.0 0.0 0.0 0.0 110-111 2.9625000000000004 0.0 0.0 0.0 0.0 112-113 3.575 0.0 0.0 0.0 0.0 114-115 4.025 0.0 0.0 0.0 0.0 116-117 4.4125 0.0 0.0 0.0 0.0 118-119 5.35 0.0 0.0 0.0 0.0 120-121 5.7875 0.0 0.0 0.0 0.0 122-123 6.3 0.0 0.0 0.0 0.0 124-125 6.7125 0.0 0.0 0.0 0.0 126-127 7.35 0.0 0.0 0.0 0.0 128-129 7.85 0.0 0.0 0.0 0.0 130-131 8.2625 0.0 0.0 0.0 0.0 132-133 9.0625 0.0 0.0 0.0 0.0 134-135 9.9125 0.0 0.0 0.0 0.0 136-137 10.55 0.0 0.0 0.0 0.0 138-139 11.3625 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AGATGGT 10 0.006830828 145.0 1 >>END_MODULE SRR26075389 read2 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR26075389_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.1785 37.0 37.0 37.0 37.0 37.0 2 36.325 37.0 37.0 37.0 37.0 37.0 3 36.3485 37.0 37.0 37.0 37.0 37.0 4 36.411 37.0 37.0 37.0 37.0 37.0 5 36.3915 37.0 37.0 37.0 37.0 37.0 6 36.3755 37.0 37.0 37.0 37.0 37.0 7 36.396 37.0 37.0 37.0 37.0 37.0 8 36.4605 37.0 37.0 37.0 37.0 37.0 9 36.3785 37.0 37.0 37.0 37.0 37.0 10-14 36.2405 37.0 37.0 37.0 37.0 37.0 15-19 36.1887 37.0 37.0 37.0 37.0 37.0 20-24 36.0757 37.0 37.0 37.0 37.0 37.0 25-29 35.8738 37.0 37.0 37.0 37.0 37.0 30-34 35.7159 37.0 37.0 37.0 37.0 37.0 35-39 35.6346 37.0 37.0 37.0 37.0 37.0 40-44 35.6078 37.0 37.0 37.0 37.0 37.0 45-49 35.57939999999999 37.0 37.0 37.0 37.0 37.0 50-54 35.3964 37.0 37.0 37.0 37.0 37.0 55-59 35.4237 37.0 37.0 37.0 37.0 37.0 60-64 35.531600000000005 37.0 37.0 37.0 37.0 37.0 65-69 35.49720000000001 37.0 37.0 37.0 37.0 37.0 70-74 35.341100000000004 37.0 37.0 37.0 37.0 37.0 75-79 35.305499999999995 37.0 37.0 37.0 37.0 37.0 80-84 35.345600000000005 37.0 37.0 37.0 37.0 37.0 85-89 35.3814 37.0 37.0 37.0 37.0 37.0 90-94 35.2505 37.0 37.0 37.0 34.6 37.0 95-99 35.4366 37.0 37.0 37.0 37.0 37.0 100-104 35.3755 37.0 37.0 37.0 37.0 37.0 105-109 35.3997 37.0 37.0 37.0 37.0 37.0 110-114 35.33 37.0 37.0 37.0 37.0 37.0 115-119 35.3314 37.0 37.0 37.0 37.0 37.0 120-124 35.17195 37.0 37.0 37.0 34.6 37.0 125-129 35.1995 37.0 37.0 37.0 34.6 37.0 130-134 35.0769 37.0 37.0 37.0 25.0 37.0 135-139 35.006299999999996 37.0 37.0 37.0 25.0 37.0 140-144 34.997550000000004 37.0 37.0 37.0 27.4 37.0 145-149 34.958099999999995 37.0 37.0 37.0 25.0 37.0 150-151 34.720375000000004 37.0 37.0 37.0 25.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.0 1101 2 0.0 1101 3 0.0 1101 4 0.0 1101 5 0.0 1101 6 0.0 1101 7 0.0 1101 8 0.0 1101 9 0.0 1101 10-14 0.0 1101 15-19 0.0 1101 20-24 0.0 1101 25-29 0.0 1101 30-34 0.0 1101 35-39 0.0 1101 40-44 0.0 1101 45-49 0.0 1101 50-54 0.0 1101 55-59 0.0 1101 60-64 0.0 1101 65-69 0.0 1101 70-74 0.0 1101 75-79 0.0 1101 80-84 0.0 1101 85-89 0.0 1101 90-94 0.0 1101 95-99 0.0 1101 100-104 0.0 1101 105-109 0.0 1101 110-114 0.0 1101 115-119 0.0 1101 120-124 0.0 1101 125-129 0.0 1101 130-134 0.0 1101 135-139 0.0 1101 140-144 0.0 1101 145-149 0.0 1101 150-151 0.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 13 9.0 14 18.0 15 10.0 16 6.0 17 6.0 18 7.0 19 2.0 20 5.0 21 8.0 22 6.0 23 11.0 24 10.0 25 16.0 26 20.0 27 20.0 28 22.0 29 20.0 30 25.0 31 26.0 32 47.0 33 96.0 34 175.0 35 636.0 36 2579.0 37 220.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 48.54927463731865 23.961980990495245 9.054527263631815 18.434217108554275 2 31.45 26.200000000000003 26.25 16.1 3 24.224999999999998 28.849999999999998 29.275000000000002 17.65 4 25.6 34.1 23.05 17.25 5 27.1 36.55 20.05 16.3 6 26.025 36.925000000000004 19.775000000000002 17.275 7 23.9 23.674999999999997 34.525 17.9 8 26.1 26.5 25.074999999999996 22.325 9 23.45 26.950000000000003 26.35 23.25 10-14 25.745 30.005 23.97 20.28 15-19 26.229999999999997 27.224999999999998 25.869999999999997 20.674999999999997 20-24 26.224999999999998 28.970000000000002 24.59 20.215 25-29 25.955000000000002 28.255000000000003 25.259999999999998 20.53 30-34 25.605 28.84 25.53 20.025000000000002 35-39 24.759999999999998 27.884999999999998 25.985000000000003 21.37 40-44 25.974999999999998 28.375 25.264999999999997 20.385 45-49 26.11 27.61 25.82 20.46 50-54 23.435 28.105000000000004 27.345000000000002 21.115000000000002 55-59 25.069999999999997 27.395000000000003 26.634999999999998 20.9 60-64 25.605 27.665 25.885 20.845 65-69 24.490000000000002 28.27 25.945 21.295 70-74 24.81 28.57 25.919999999999998 20.7 75-79 24.55 29.415000000000003 26.145000000000003 19.89 80-84 24.495 28.46 26.87 20.175 85-89 25.825 27.965 26.33 19.88 90-94 25.535000000000004 26.979999999999997 27.405 20.080000000000002 95-99 25.330000000000002 28.499999999999996 25.874999999999996 20.294999999999998 100-104 25.569999999999997 27.150000000000002 25.619999999999997 21.66 105-109 24.485 28.025 26.889999999999997 20.599999999999998 110-114 25.555 28.255000000000003 25.240000000000002 20.95 115-119 25.014999999999997 28.18 26.56 20.244999999999997 120-124 25.211260563028155 27.651382569128458 26.686334316715836 20.451022551127558 125-129 26.06 28.455000000000002 25.124999999999996 20.36 130-134 26.064999999999998 28.985 24.375 20.575 135-139 26.9503900780156 29.195839167833565 24.40988197639528 19.443888777755554 140-144 26.359225729005153 29.16520782273796 25.11879157705197 19.35677487120492 145-149 26.853426713356676 29.989994997498748 24.502251125562783 18.65432716358179 150-151 27.060147555333252 28.223083656371138 25.28448168063024 19.432287107665374 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.5 6 0.5 7 0.5 8 0.5 9 0.0 10 0.0 11 0.0 12 0.5 13 0.5 14 1.0 15 2.0 16 1.0 17 0.0 18 0.5 19 0.5 20 0.0 21 0.5 22 1.5 23 2.0 24 1.5 25 3.5 26 4.5 27 4.0 28 3.5 29 5.5 30 7.5 31 10.0 32 16.5 33 24.0 34 37.5 35 43.0 36 62.0 37 76.0 38 104.0 39 135.5 40 170.0 41 229.5 42 230.0 43 246.0 44 283.5 45 286.0 46 263.0 47 247.0 48 239.5 49 219.0 50 188.0 51 167.5 52 146.5 53 98.5 54 71.5 55 56.5 56 50.0 57 38.0 58 24.0 59 23.5 60 21.0 61 20.5 62 12.0 63 4.5 64 6.0 65 5.5 66 5.0 67 5.0 68 3.5 69 1.0 70 2.5 71 2.5 72 0.0 73 1.0 74 2.0 75 1.5 76 1.5 77 3.0 78 2.5 79 2.0 80 3.0 81 3.0 82 2.5 83 2.0 84 3.0 85 4.0 86 5.5 87 6.0 88 3.5 89 3.5 90 5.0 91 3.0 92 2.0 93 2.5 94 1.5 95 1.5 96 1.0 97 0.0 98 1.0 99 2.0 100 7.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.05 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.005 125-129 0.0 130-134 0.0 135-139 0.02 140-144 0.034999999999999996 145-149 0.05 150-151 0.0375 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 60.099999999999994 #Duplication Level Percentage of deduplicated Percentage of total 1 62.562396006655575 37.6 2 22.420965058236273 26.950000000000003 3 7.9866888519134775 14.399999999999999 4 4.159733777038269 10.0 5 1.5806988352745424 4.75 6 0.49916805324459235 1.7999999999999998 7 0.33277870216306155 1.4000000000000001 8 0.20798668885191346 1.0 9 0.04159733777038269 0.22499999999999998 >10 0.20798668885191346 1.875 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGT 24 0.6 No Hit GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG 20 0.5 No Hit AGCCAATAAGAAGGGGATTGGAGAGAAACATGTTCCTATTACCATAGGTG 11 0.27499999999999997 No Hit GTTCAAGAGAAATTTGGAGACGAGAGGAGAGAAGGAAAAGGTTAGCGATG 10 0.25 No Hit GGCTTTTGGTGACTACTTTCCAGGCGTGGTAAATCCCCTTGATGGGATAC 10 0.25 No Hit GCAGAGGGAGGGAGCCTGCCCTTGAGAAAGTGAGAGGGAGGTGGGGTTTA 9 0.22499999999999998 No Hit GGTGGTGAAGAGAAAGTGAGAAGGGCGAAGAGGAGGAGGAGGGCATGATG 8 0.2 No Hit ATCAGGCTACCGAATAGCCGGGAAAGAATTTGGTTAGGATCATATGATTC 8 0.2 No Hit GTTCCATAATCCCTTCCAAATCCTTGATCCCCTCCAACAAACCCCATCAG 8 0.2 No Hit ACTGGTTTACCAGAAATGGAGAACAGGCCAAAACTACATGTCGTTCAAGC 8 0.2 No Hit CAGAATCAAAACCTTTGTACAGAACTTCCTCATATCCCCAGCAGCCACAA 8 0.2 No Hit CAACAACAACGACAACAAGATGCCTCGAGACGACAGCCCACCGCCGCCTC 7 0.17500000000000002 No Hit AGAAATTTCAGTATGATCCAAAAACAGACTCACCAAGCGATGCTAGAAAT 7 0.17500000000000002 No Hit GGCCAAGTGCACTCAGTGCAAAAGATGAGATGGCAAGATTGTCACGGCAT 7 0.17500000000000002 No Hit TGAGTACCGAATGGAGCTGATGGAACTTCTATCCTTGACAAGCCATGAAG 7 0.17500000000000002 No Hit GCGGAAGTCTTGTGCCTGTTGATGAAGGTTCTCAGTCTTGTCCACAAGAA 7 0.17500000000000002 No Hit AATTACCTCCCGAGATTGGAGATAGACCCGGCCCTAAACCGGCTCTTTAT 7 0.17500000000000002 No Hit GTCAGATGGGGTTGTCGTTTGATCGGGCCACGGTGGAGGGGAGGAGACAG 7 0.17500000000000002 No Hit GGTTAAAGTCAAAAGCTTTATAAAACCCTAGCTAGCTAACCAAGCTCTCT 7 0.17500000000000002 No Hit ATTTGGTGAAGAGAGTTGAGGAGTTTGCCAAGGAGGAAAGGTTGAGGGTG 6 0.15 No Hit CGGTACTTCAGCTGCCCCAACCTACCTCCCTGCCCATTATTTTGAAACCA 6 0.15 No Hit GAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATAGGAGGTTTCCTCC 6 0.15 No Hit CAGCGATATTCAGTACACATAAATTGTGGGGGACCAGAAGCCACCATTGG 6 0.15 No Hit CTACTGATCGTTCTTCTTGCTTTGAGCGCAATAAGGTTGCACTCCGAATG 6 0.15 No Hit CTAGAAGTTTTGGAGAAAATGAAACTGAAGCCAATACTAATAAAGTGGCT 6 0.15 No Hit GTAAGATACAAGAACTAAAGCAATCAGTCTTGCAAAGAATGATACCATTA 6 0.15 No Hit GATGCACGTTACTGGAAATGGCCTTCCATATGTGAATCAGGTGATGTCAC 6 0.15 No Hit AGAAGCACAAAGAGAGAGGAAGAGAAAGAGAAGGAAGCAATGGCAGCGAT 6 0.15 No Hit ATTTAGAGCTGCACAAAGTTGGTTCAAGGTATGATAACTTAAGTACAACA 6 0.15 No Hit TGCAGAGATGACGGGCCGAGTTGATTTTGACAAGAGTGTGGAATATTGGC 6 0.15 No Hit GTAATATGCCCTACTGTGCGTGCAAAACAAGCAGGAAAACACGCTTTCCC 6 0.15 No Hit GGAGGGCATCCCTCCAGACCAACAGAGACTCATCTTTGCTGGGAAGCAAC 5 0.125 No Hit CGAAAGGGCGTGCATCCTACACCATGCAATTAGCCAAGTTTGATGTCGTC 5 0.125 No Hit ACCAGCTTGAGCAAATTCAGTTTCTAAGCAAAAGCTTTCCAGGCCCCTTT 5 0.125 No Hit TGCAATTTCATTCAACAGCTAAGGTGTGGAGGGAACCAGGGCTTTGTGGA 5 0.125 No Hit CTCTCTCTCTCTCTCCTTCCCAAAAGCAACAAGGAAACAAACGACAAGGA 5 0.125 No Hit TCTAAGCCAGAGCATTTTCTATTTGAAGCTCTTGCGTTCTCAGCCTTTCT 5 0.125 No Hit GGAAATTCAACCGAGTATGGTGGGAACAATGATGGTAAATAGGGTCACTT 5 0.125 No Hit GTTTCGTTAAGGTTGTTAACTATCAGCACCTGATGCCTACTAGGTATACT 5 0.125 No Hit GTTCTTGTTAGACAGAGAGATGGAGAAGAAATGCTATGGTCTTTTCTTGT 5 0.125 No Hit CCAGAGGCCATTCATGAGGGGGAAACTGTACATTCATTTCACTGTTGATT 5 0.125 No Hit CCTGGATCAAAAAACAAGCCCAAACCGCCAATTTTTGTGACTCGGGACAG 5 0.125 No Hit CGAGAGGAGCGGCTCTTCTTCATTTGCTTCGATCTCAAAGCAAACAGCTC 5 0.125 No Hit GAGAGGTCAAATCGAGTTTATAATTACACATTAGATCCATGTGGTCCTGT 5 0.125 No Hit GGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAGTCGGGTTGC 5 0.125 No Hit GAGTTGGGAAACTTGACAAACTTGGTGAATCTTGACCTCTCGTACAATGC 5 0.125 No Hit GTGAAGTTGTTTCTGTGAAAATGCCAACTGGAAAAGGATGTGCATTTGTA 5 0.125 No Hit CAGCCAGTGAAATAGATTTAAGTTTTGATAAAAGTCCAGAACAACGTGCT 5 0.125 No Hit GGGACTTTTAAATCCTCCTCTTAATGAACAAATCAGACCTCTAAGATTGC 5 0.125 No Hit AGGAACTCCACCTCACAAGGGGAAGAGCTGATTGATTTGCACAGCATTAA 5 0.125 No Hit GGTTTTTAAGGAAAAACAAACTTGCTGTCTGGTCTTTCTATGTTTTATTG 5 0.125 No Hit GTGAAGCGGTTACTACTATCAAAACTGGTGTCAATGAGAAGCCTCGCAAG 5 0.125 No Hit GTGATGTCCAAATCTGAGGTTTAATGGGTACTCCGGTCAATTCAAGTGTT 5 0.125 No Hit GACGTCTAAGGGCGGTGTACACCCTTTTGAGCAATGATTGCACAACCTGC 5 0.125 No Hit ACCAAACGCTCCCAATCTCCGCCATGTCTGCCTCCAAACAGGAGGGAAAC 5 0.125 No Hit GAGGGCATTATCAAGAGAGGAGCAGCAAACAAATGCCAGGTCTGGAAGCT 5 0.125 No Hit TGTTAATGTGAATAGTAATGGAATCAAGAGTGAATTGTCAAAGAAGAGTA 5 0.125 No Hit GGTCAGGCGGGACTACCCGCTGAGTTTAAGCATATCAATAAGCGGAGGAA 5 0.125 No Hit TGGAGGCAGCACTGGACCCGGAAGAGCATGGGAAAGGAATGGACATGTGC 5 0.125 No Hit AAACAGACGTTTTTCTGTTCTTCATATTATCGAGAGAGAAAATGGCCGAA 5 0.125 No Hit GGTAACAATATCAACATCGAAACTGTGATCCAAGAAACTACGATCAATAT 5 0.125 No Hit CAGACCTGGAGCCAGCATTGAAAGCTCTCAAGGATAGGCTCATGACCAAG 5 0.125 No Hit TGGGGATGTGCAGAGTGATTTCTTGGCCCAAGGTTTTGGATCTCTTGGCT 5 0.125 No Hit GATTGAGAAACTATGAGGTATTTCTTGCAGAGCTTGACAGTATTCAAAGT 5 0.125 No Hit GAACTGCTCAAGACAGCGATAGCCAAAGCTGGATATACTGGAAAGGTTGT 5 0.125 No Hit CATAAGGAAGCTTGTGAAGGATGGTTTCATTATCAGAAAGCCAACTAAGA 5 0.125 No Hit GTCTTAAAAAGGGGTGGGATATGGAGGGATATATACGGGATTGATGAGAA 5 0.125 No Hit ACACCATGCAAAGGCCTAAGCCTATCAGAACCAGCATTTCTGCTAGGCCA 5 0.125 No Hit GTTCGAGCTGAGTCTGGTTTAGTTGCTCAGCCACATGTGCAAGAAAGGGT 5 0.125 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0125 0.0 0.0 0.0 0.0 50-51 0.025 0.0 0.0 0.0 0.0 52-53 0.025 0.0 0.0 0.0 0.0 54-55 0.025 0.0 0.0 0.0 0.0 56-57 0.025 0.0 0.0 0.0 0.0 58-59 0.05 0.0 0.0 0.0 0.0 60-61 0.05 0.0 0.0 0.0 0.0 62-63 0.05 0.0 0.0 0.0 0.0 64-65 0.0625 0.0 0.0 0.0 0.0 66-67 0.075 0.0 0.0 0.0 0.0 68-69 0.075 0.0 0.0 0.0 0.0 70-71 0.075 0.0 0.0 0.0 0.0 72-73 0.075 0.0 0.0 0.0 0.0 74-75 0.15 0.0 0.0 0.0 0.0 76-77 0.23750000000000002 0.0 0.0 0.0 0.0 78-79 0.3 0.0 0.0 0.0 0.0 80-81 0.3 0.0 0.0 0.0 0.0 82-83 0.3125 0.0 0.0 0.0 0.0 84-85 0.325 0.0 0.0 0.0 0.0 86-87 0.38749999999999996 0.0 0.0 0.0 0.0 88-89 0.5125 0.0 0.0 0.0 0.0 90-91 0.6499999999999999 0.0 0.0 0.0 0.0 92-93 0.75 0.0 0.0 0.0 0.0 94-95 0.8 0.0 0.0 0.0 0.0 96-97 0.9 0.0 0.0 0.0 0.0 98-99 1.025 0.0 0.0 0.0 0.0 100-101 1.0875 0.0 0.0 0.0 0.0 102-103 1.4125 0.0 0.0 0.0 0.0 104-105 1.85 0.0 0.0 0.0 0.0 106-107 2.3 0.0 0.0 0.0 0.0 108-109 2.6125 0.0 0.0 0.0 0.0 110-111 2.9375 0.0 0.0 0.0 0.0 112-113 3.5375 0.0 0.0 0.0 0.0 114-115 3.975 0.0 0.0 0.0 0.0 116-117 4.3625 0.0 0.0 0.0 0.0 118-119 5.175 0.0 0.0 0.0 0.0 120-121 5.5875 0.0 0.0 0.0 0.0 122-123 6.125 0.0 0.0 0.0 0.0 124-125 6.5375 0.0 0.0 0.0 0.0 126-127 7.175 0.0 0.0 0.0 0.0 128-129 7.675000000000001 0.0 0.0 0.0 0.0 130-131 8.15 0.0 0.0 0.0 0.0 132-133 8.9625 0.0 0.0 0.0 0.0 134-135 9.75 0.0 0.0 0.0 0.0 136-137 10.3875 0.0 0.0 0.0 0.0 138-139 11.225000000000001 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTCATGA 10 0.006830828 145.0 4 >>END_MODULE Read 983138 spots for SRR26075389.sra Written 983138 spots for SRR26075389.sra Read 983138 spots for SRR26075389.sra Written 983138 spots for SRR26075389.sra Read 983138 spots for SRR26075389.sra Written 983138 spots for SRR26075389.sra Read 983138 spots for SRR26075389.sra Written 983138 spots for SRR26075389.sra Read 983138 spots for SRR26075389.sra Written 983138 spots for SRR26075389.sra Read 983138 spots for SRR26075389.sra Written 983138 spots for SRR26075389.sra Read 983138 spots for SRR26075389.sra Written 983138 spots for SRR26075389.sra Read 983138 spots for SRR26075389.sra Written 983138 spots for SRR26075389.sra Read 983138 spots for SRR26075389.sra Written 983138 spots for SRR26075389.sra Read 983145 spots for SRR26075389.sra Written 983145 spots for SRR26075389.sra Read 983138 spots for SRR26075389.sra Written 983138 spots for SRR26075389.sra Read 983138 spots for SRR26075389.sra Written 983138 spots for SRR26075389.sra Read 983138 spots for SRR26075389.sra Written 983138 spots for SRR26075389.sra Read 983138 spots for SRR26075389.sra Written 983138 spots for SRR26075389.sra Read 983138 spots for SRR26075389.sra Written 983138 spots for SRR26075389.sra Read 983138 spots for SRR26075389.sra Written 983138 spots for SRR26075389.sra Read 983138 spots for SRR26075389.sra Written 983138 spots for SRR26075389.sra Read 983138 spots for SRR26075389.sra Written 983138 spots for SRR26075389.sra Read 983138 spots for SRR26075389.sra Written 983138 spots for SRR26075389.sra Read 983138 spots for SRR26075389.sra Written 983138 spots for SRR26075389.sra SRR ids: ['SRR26075389.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_gm74g7jf SRR26075389.sra spots: 19662767 blocks: [[1, 983138], [983139, 1966276], [1966277, 2949414], [2949415, 3932552], [3932553, 4915690], [4915691, 5898828], [5898829, 6881966], [6881967, 7865104], [7865105, 8848242], [8848243, 9831380], [9831381, 10814518], [10814519, 11797656], [11797657, 12780794], [12780795, 13763932], [13763933, 14747070], [14747071, 15730208], [15730209, 16713346], [16713347, 17696484], [17696485, 18679622], [18679623, 19662767]] SRR26075389 file size 7256418 SRR26075389 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o SRR26075389 SRR26075389_1.fastq SRR26075389_2.fastq Input file: SRR26075389_1.fastq Paired file: SRR26075389_2.fastq trimmed: SRR26075389-trimmed-pair1.fastq, SRR26075389-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Wed Feb 12 00:13:50 2025 >> started Wed Feb 12 00:14:11 2025 >> done (21.339s) 19662767 read pairs processed; of these: 114 ( 0.00%) short read pairs filtered out after trimming by size control 145339 ( 0.74%) empty read pairs filtered out after trimming by size control 19517314 (99.26%) read pairs available; of these: 3080056 (15.78%) trimmed read pairs available after processing 16437258 (84.22%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 18 7 0.00% 19 14 0.00% 20 18 0.00% 21 21 0.00% 22 30 0.00% 23 23 0.00% 24 30 0.00% 25 29 0.00% 26 12 0.00% 27 42 0.00% 28 23 0.00% 29 27 0.00% 30 25 0.00% 31 38 0.00% 32 40 0.00% 33 45 0.00% 34 43 0.00% 35 25 0.00% 36 35 0.00% 37 31 0.00% 38 40 0.00% 39 61 0.00% 40 57 0.00% 41 52 0.00% 42 55 0.00% 43 73 0.00% 44 71 0.00% 45 81 0.00% 46 76 0.00% 47 85 0.00% 48 117 0.00% 49 224 0.00% 50 109 0.00% 51 114 0.00% 52 178 0.00% 53 159 0.00% 54 146 0.00% 55 185 0.00% 56 232 0.00% 57 278 0.00% 58 301 0.00% 59 387 0.00% 60 376 0.00% 61 512 0.00% 62 606 0.00% 63 606 0.00% 64 753 0.00% 65 815 0.00% 66 891 0.00% 67 950 0.00% 68 1128 0.01% 69 1247 0.01% 70 1611 0.01% 71 1712 0.01% 72 1951 0.01% 73 2415 0.01% 74 2748 0.01% 75 2929 0.02% 76 3337 0.02% 77 3458 0.02% 78 4116 0.02% 79 4454 0.02% 80 5125 0.03% 81 5804 0.03% 82 6534 0.03% 83 7090 0.04% 84 7863 0.04% 85 8952 0.05% 86 9133 0.05% 87 10310 0.05% 88 11069 0.06% 89 11820 0.06% 90 12255 0.06% 91 13421 0.07% 92 14588 0.07% 93 15909 0.08% 94 17149 0.09% 95 18219 0.09% 96 19777 0.10% 97 20436 0.10% 98 20913 0.11% 99 22349 0.11% 100 23172 0.12% 101 24294 0.12% 102 25364 0.13% 103 27031 0.14% 104 29135 0.15% 105 30630 0.16% 106 31477 0.16% 107 32603 0.17% 108 33656 0.17% 109 34357 0.18% 110 35155 0.18% 111 36555 0.19% 112 37378 0.19% 113 38943 0.20% 114 41005 0.21% 115 42790 0.22% 116 44304 0.23% 117 45194 0.23% 118 46053 0.24% 119 46534 0.24% 120 47547 0.24% 121 49289 0.25% 122 50834 0.26% 123 51911 0.27% 124 54681 0.28% 125 55596 0.28% 126 56787 0.29% 127 58085 0.30% 128 58376 0.30% 129 60504 0.31% 130 61591 0.32% 131 61800 0.32% 132 63012 0.32% 133 63902 0.33% 134 65916 0.34% 135 68244 0.35% 136 69081 0.35% 137 69129 0.35% 138 71168 0.36% 139 71815 0.37% 140 72750 0.37% 141 74533 0.38% 142 75789 0.39% 143 75829 0.39% 144 78084 0.40% 145 79153 0.41% 146 80797 0.41% 147 81097 0.42% 148 82347 0.42% 149 81493 0.42% 150 84316 0.43% 151 16437258 84.22% 19517314 reads passed initial QC criterion=sequence-density sequence-density=0.28 sequence-density-rank=1 fanout-score=2.87 fanout-score-rank=35 prefix-density=0.31 prefix-fanout=2.6 sequence=AGGAAACCTCCT criterion=fanout-score sequence-density=0.03 sequence-density-rank=37 fanout-score=95.25 fanout-score-rank=1 prefix-density=0.35 prefix-fanout=7.5 sequence=CATCACCATCCCTGATTGATCTTTGATTCACAACAAGACCAACCAACCGTACAATGTTTACATAACTTGGGATAATCTCACAAGAAAGAAAGATCAATACAAAACTAGTCAGCTCAGGATGTTCCAGGCACGGTAGAACCGTGGAACCATAACAACAAGAGACATATTGCAGATAAGTACTGAAAAACAAAACACAGTACGTATTTACATGGGCAACCTTGGTTGAAGGCAACCTCATCAACGATGCTCGCTCTTCGTCTCTCCACTGTACATCCAGTCATAGACAGTGGGTGTGTTAGGCTGTGGCTTGTCAAAAACATGAGCACCAATATTCTTAGTAGCAAGGTTGCTACCAGGGTGGAACACGCTCCTCCAAACATTGCTACGCGCCGACACTGGGGTTGTAGGGGTCACTGGTGTCGTCGGTGTCCCTGGAGTTCCTGGCATAGTCATGGACCTCTGAAACTTATTAACAGGACTGCTCCCCTCTCCGACGTCAATATCTTTGATG criterion=sequence-density sequence-density=0.39 sequence-density-rank=1 fanout-score=2.40 fanout-score-rank=34 prefix-density=0.41 prefix-fanout=2.3 sequence=TGGTTTTACTAG criterion=fanout-score sequence-density=0.04 sequence-density-rank=35 fanout-score=124.34 fanout-score-rank=1 prefix-density=0.39 prefix-fanout=11.6 sequence=AAACCCTAAAAGCTCAAACCTTTGGCGGCTATCCACTGAACCCACCAAAGGGTTTTATTATCTCACAAGATCTGTCATGGCTACCCTTTCATCTTCTGCTGACCAAACTTCAGATTTGCTGCAGAAGTTATCCTTGGACTCACAAAC SRR26075389 testing PE reads STAR mapping to Ensembl genome Started job on | Feb 12 00:15:02 Started mapping on | Feb 12 00:15:02 Finished on | Feb 12 00:18:42 Mapping speed, Million of reads per hour | 319.37 Number of input reads | 19517314 Average input read length | 294 UNIQUE READS: Uniquely mapped reads number | 17088790 Uniquely mapped reads % | 87.56% Average mapped length | 292.41 Number of splices: Total | 16240709 Number of splices: Annotated (sjdb) | 15844985 Number of splices: GT/AG | 15930343 Number of splices: GC/AG | 239887 Number of splices: AT/AC | 16756 Number of splices: Non-canonical | 53723 Mismatch rate per base, % | 0.40% Deletion rate per base | 0.03% Deletion average length | 3.10 Insertion rate per base | 0.02% Insertion average length | 2.63 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 483249 % of reads mapped to multiple loci | 2.48% Number of reads mapped to too many loci | 109632 % of reads mapped to too many loci | 0.56% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 8.95% % of reads unmapped: other | 0.46% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 1945275 1945275 1945275 N_multimapping 483249 483249 483249 N_noFeature 514202 16917438 607337 N_ambiguous 173080 1049 94217 UnstrandedReadsAssigned:16401508 PositiveStrandReadsAssigned:170303 NegativeStrandReadsAssigned:16387236 Dataset is classified negative stranded MeadianReadLen=151 20thPercentileLength=151 echo kmer=147 SRR26075389 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,400 [index] number of k-mers: 62,057,036 [index] number of equivalence classes: 130,681 [quant] running in paired-end mode [quant] will process pair 1: SRR26075389-trimmed-pair1.fastq SRR26075389-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 19,517,314 reads, 16,684,637 reads pseudoaligned [quant] estimated average fragment length: 214.361 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,131 rounds 52401 SRR26075389.ke.tsv 34699 SRR26075389.se.tsv 87100 total ==> SRR26075389.ke.tsv <== target_id length eff_length est_counts tpm Potri.005G200100.1.v4.1 2018 1804.64 1431 45.4665 Potri.005G024800.1.v4.1 1035 821.639 918 64.0624 Potri.004G059700.1.v4.1 961 747.645 1 0.0766915 Potri.007G009000.2.v4.1 1416 1202.64 0 0 Potri.003G141000.2.v4.1 2943 2729.64 732 15.3762 Potri.016G087400.1.v4.1 270 90.1737 1494 949.976 Potri.015G069301.1.v4.1 564 352.687 0 0 Potri.010G195200.1.v4.1 1773 1559.64 320 11.7643 Potri.012G127500.1.v4.1 977 763.639 6441 483.623 ==> SRR26075389.se.tsv <== Potri.001G166300.v4.1 0 Potri.001G448400.v4.1 110 Potri.001G233950.v4.1 0 Potri.001G122700.v4.1 193 Potri.001G212900.v4.1 0 Potri.001G182400.v4.1 0 Potri.001G256600.v4.1 0 Potri.001G040500.v4.1 0 Potri.001G416900.v4.1 0 Potri.001G452600.v4.1 799 SRR26075389 completed mapping pipeline successfully