Starting /dee2/code/volunteer_pipeline.sh SRR26075390 current disk space = 3051701047296 free memory = 1476249860 SRR26075390 SRAfilesize d9e4db91e842662bacca329aa3082ea5 SRR26075390.sra SRR26075390.sra file validated SRR26075390 is paired end SRR26075390 is conventional basespace SRR26075390 read1 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR26075390_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.5905 37.0 37.0 37.0 37.0 37.0 2 36.6535 37.0 37.0 37.0 37.0 37.0 3 36.6615 37.0 37.0 37.0 37.0 37.0 4 36.651 37.0 37.0 37.0 37.0 37.0 5 36.651 37.0 37.0 37.0 37.0 37.0 6 36.6755 37.0 37.0 37.0 37.0 37.0 7 36.5305 37.0 37.0 37.0 37.0 37.0 8 36.6195 37.0 37.0 37.0 37.0 37.0 9 36.627 37.0 37.0 37.0 37.0 37.0 10-14 36.61645 37.0 37.0 37.0 37.0 37.0 15-19 36.611 37.0 37.0 37.0 37.0 37.0 20-24 36.562799999999996 37.0 37.0 37.0 37.0 37.0 25-29 36.521100000000004 37.0 37.0 37.0 37.0 37.0 30-34 36.427499999999995 37.0 37.0 37.0 37.0 37.0 35-39 36.412699999999994 37.0 37.0 37.0 37.0 37.0 40-44 36.419799999999995 37.0 37.0 37.0 37.0 37.0 45-49 36.2934 37.0 37.0 37.0 37.0 37.0 50-54 36.3249 37.0 37.0 37.0 37.0 37.0 55-59 36.222699999999996 37.0 37.0 37.0 37.0 37.0 60-64 36.1764 37.0 37.0 37.0 37.0 37.0 65-69 36.13 37.0 37.0 37.0 37.0 37.0 70-74 36.043600000000005 37.0 37.0 37.0 37.0 37.0 75-79 35.99550000000001 37.0 37.0 37.0 37.0 37.0 80-84 36.044599999999996 37.0 37.0 37.0 37.0 37.0 85-89 35.8574 37.0 37.0 37.0 37.0 37.0 90-94 35.836200000000005 37.0 37.0 37.0 37.0 37.0 95-99 35.892900000000004 37.0 37.0 37.0 37.0 37.0 100-104 35.8677 37.0 37.0 37.0 37.0 37.0 105-109 35.805899999999994 37.0 37.0 37.0 37.0 37.0 110-114 35.630900000000004 37.0 37.0 37.0 37.0 37.0 115-119 35.5595 37.0 37.0 37.0 37.0 37.0 120-124 35.5383 37.0 37.0 37.0 37.0 37.0 125-129 35.430400000000006 37.0 37.0 37.0 37.0 37.0 130-134 35.2959 37.0 37.0 37.0 32.2 37.0 135-139 35.2265 37.0 37.0 37.0 32.2 37.0 140-144 35.0049 37.0 37.0 37.0 25.0 37.0 145-149 34.9393 37.0 37.0 37.0 25.0 37.0 150-151 34.826750000000004 37.0 37.0 37.0 25.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.0 1101 2 0.0 1101 3 0.0 1101 4 0.0 1101 5 0.0 1101 6 0.0 1101 7 0.0 1101 8 0.0 1101 9 0.0 1101 10-14 0.0 1101 15-19 0.0 1101 20-24 0.0 1101 25-29 0.0 1101 30-34 0.0 1101 35-39 0.0 1101 40-44 0.0 1101 45-49 0.0 1101 50-54 0.0 1101 55-59 0.0 1101 60-64 0.0 1101 65-69 0.0 1101 70-74 0.0 1101 75-79 0.0 1101 80-84 0.0 1101 85-89 0.0 1101 90-94 0.0 1101 95-99 0.0 1101 100-104 0.0 1101 105-109 0.0 1101 110-114 0.0 1101 115-119 0.0 1101 120-124 0.0 1101 125-129 0.0 1101 130-134 0.0 1101 135-139 0.0 1101 140-144 0.0 1101 145-149 0.0 1101 150-151 0.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 19 1.0 20 1.0 21 0.0 22 2.0 23 2.0 24 6.0 25 6.0 26 12.0 27 7.0 28 21.0 29 25.0 30 36.0 31 41.0 32 64.0 33 101.0 34 175.0 35 482.0 36 2821.0 37 197.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 47.21664994984955 12.487462387161484 5.541624874623872 34.7542627883651 2 19.400000000000002 13.475000000000001 33.775 33.35 3 16.175 19.7 28.799999999999997 35.325 4 20.525 25.224999999999998 26.924999999999997 27.325 5 23.75 30.349999999999998 24.95 20.95 6 23.225 36.6 20.325 19.85 7 15.950000000000001 28.1 39.5 16.45 8 17.375 26.575 32.1 23.95 9 17.724999999999998 24.3 34.325 23.65 10-14 20.133019952992946 29.209381407211083 27.839175876381457 22.81842276341451 15-19 20.06 28.215 27.855 23.87 20-24 19.900000000000002 27.975 28.044999999999998 24.08 25-29 20.21 27.700000000000003 28.215 23.875 30-34 20.25 28.18 27.779999999999998 23.79 35-39 19.935 27.400000000000002 28.77 23.895 40-44 20.32 28.025 27.474999999999998 24.18 45-49 20.89 26.815 28.07 24.224999999999998 50-54 20.055 27.689999999999998 27.975 24.279999999999998 55-59 20.16 27.845 27.815 24.18 60-64 20.18 27.93 28.03 23.86 65-69 20.75 27.650000000000002 27.61 23.990000000000002 70-74 20.435 27.884999999999998 27.785 23.895 75-79 20.815 27.779999999999998 27.67 23.735 80-84 20.225 26.58 28.76 24.435000000000002 85-89 21.325 27.07 27.54 24.065 90-94 21.525 27.189999999999998 27.474999999999998 23.810000000000002 95-99 20.805 27.065 27.810000000000002 24.32 100-104 21.05 27.49 27.750000000000004 23.71 105-109 21.38 27.115000000000002 27.700000000000003 23.805 110-114 21.665 26.945000000000004 27.72 23.669999999999998 115-119 21.52 27.560000000000002 27.139999999999997 23.78 120-124 21.365000000000002 27.060000000000002 27.375 24.2 125-129 21.345 28.015 26.47 24.169999999999998 130-134 21.224999999999998 28.365000000000002 25.990000000000002 24.42 135-139 20.875 27.49 27.125 24.51 140-144 21.625 26.650000000000002 27.139999999999997 24.585 145-149 21.475 27.334999999999997 26.645000000000003 24.545 150-151 22.175 27.6375 26.474999999999998 23.7125 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.5 8 1.0 9 0.5 10 0.0 11 0.0 12 0.0 13 0.5 14 0.5 15 0.0 16 0.0 17 0.0 18 1.0 19 1.0 20 0.0 21 3.0 22 4.0 23 1.5 24 2.0 25 3.5 26 3.0 27 4.5 28 8.0 29 7.0 30 14.5 31 27.0 32 33.0 33 35.5 34 39.0 35 55.0 36 73.0 37 96.5 38 128.5 39 146.5 40 168.5 41 216.0 42 251.0 43 252.5 44 265.0 45 260.5 46 246.5 47 264.5 48 245.0 49 203.5 50 169.0 51 143.0 52 137.5 53 112.0 54 82.5 55 60.5 56 45.0 57 34.5 58 22.5 59 21.0 60 22.0 61 20.0 62 11.0 63 12.0 64 12.5 65 5.0 66 5.0 67 7.5 68 7.5 69 3.5 70 1.0 71 0.5 72 0.0 73 0.5 74 0.5 75 0.5 76 0.5 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.015 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150-151 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 61.224999999999994 #Duplication Level Percentage of deduplicated Percentage of total 1 62.882809309922415 38.5 2 21.641486320947326 26.5 3 9.269089424254798 17.025000000000002 4 3.552470396080033 8.7 5 1.5108207431604737 4.625 6 0.7758268681094325 2.85 7 0.12249897917517355 0.525 8 0.16333197223356472 0.8 9 0.04083299305839118 0.22499999999999998 >10 0.04083299305839118 0.25 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GCATCAGCAAGTTTGCAAAAAATGCCAGACCCACAAAGAGGAACAGAGGC 10 0.25 No Hit GCTTAATTGATGGGTGAATGTTTTCACTCGTCATCTTCATCATCAGCAGC 9 0.22499999999999998 No Hit CATCTCAGGTGTATCACTTCCCGACTGTTTAGCAGGAATTTCAGATAGGC 8 0.2 No Hit GTTTGGATGATAAACTTTTGTCTTAAAGGAAACCTTGGGTGGCTTGAATG 8 0.2 No Hit GTTAAACCAACATTAATACCACAACTATCTTAATTGCCACTGACTAGCAA 8 0.2 No Hit GGTCCGTCAACAAAGAGCAGATGGAGAGCAATACCTTGGATATGGTTAGA 8 0.2 No Hit GCCAAATCTGTACTTGAAGGAAGATATACTTTAATCTGTCCCTGTAGCTA 7 0.17500000000000002 No Hit CTCTGAAATATCAGTCATGAACTGATCTGTATCCCATCCAATCTGAGGGT 7 0.17500000000000002 No Hit CCGGAGCTTGCCGAGACCGCTTTCCGGAAGGGGACCGGCAACGGTGTCGT 7 0.17500000000000002 No Hit CTCTGGTTTTGATATGCCAAAGCCAACAGCTACAGGTTTGGTTGTTGTCT 6 0.15 No Hit CAACCAACAAAACACAACAGAATTCAAGCAAACAATCTAAGCTATATTAA 6 0.15 No Hit GCCATCACTGTTCTAAGCTACATCCCTCCAACAAAATCTTGGAAAGTTAG 6 0.15 No Hit GTCACGTTGAATATTCACTGCCCATTCGAATCTGTTTAAATCAGCGTGGC 6 0.15 No Hit GGAACGTCAGCTAACATAACCCCACAAGTAAGGTTCCGGAATAGCATTTG 6 0.15 No Hit GTCAAAATCATAGCACTTATCTCTTCAGGACTGAAGACCTTAGTCTCCCC 6 0.15 No Hit CCTCAAACAATTCTTGACTGGTGGGATCTTTAAGACTAATGGATTGCAAA 6 0.15 No Hit CCCAACGACCTGAAAAATGCGATCATATTGTTGTAAACATCTTCCTTTCC 6 0.15 No Hit GGTGGGAGTAGGGATTTGTGCTGTTTGTAACACAAAGTGAGGCATTGCTC 6 0.15 No Hit GCACAGAATCCAACCCACAAAAAAATAAGAGAAAGAAACTCACTGCCCAA 6 0.15 No Hit CCCAAAGTCATAGTTTTTGTACTTCTCCAACATGGCAGACATCCCCTTGC 6 0.15 No Hit GCATGGTATAAAGAAGTTGGCACTAGCGTCTCATATGGTTCATTCAGTGT 6 0.15 No Hit GGTGTTTAGTTTGTTCATGGCATTGAGGTTGAGGGTGGCCTCCTCCTCAC 6 0.15 No Hit CTTCCTTGCTCCCACAACTTTGTCCACAATCTTCCCCTCTTTCAGGAACA 6 0.15 No Hit GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGAACTGTATCTCGTAT 6 0.15 TruSeq Adapter, Index 22 (97% over 38bp) GTTGGGAGAGATCACCAGGGCATTCACAAACTATCAACAAGTCCATTCCT 6 0.15 No Hit GCTATCATTGTTTAAAACCAGAGCAGTAATATCCTGGCCTTCTCCAAGTT 6 0.15 No Hit CCTCATTATAATGTCCGATGTAGAAATGCCATCAGTTCTTTTCGTTTCCT 6 0.15 No Hit GCTAAGATAAACGACAAACACCAATCCATACAGTGTGGACATGGAGGTTA 6 0.15 No Hit GTTTCTCCTTCAGCTTTCACAACAGGCCCTTTCTTCAAAGGTTTCGCCTT 5 0.125 No Hit CGCTGACAGTGATTTCCATTTATCTCCTCCAGCTTTTCCAACAGCAGCAA 5 0.125 No Hit CACCACATAATTTTTGTTATCATAAGCTAGTGTGAAGAACACACCAAACC 5 0.125 No Hit GTCTTAGTAGCTTCATTGGCTCCTTTCTTGAGTTGCTTGTAGTTAGCAGC 5 0.125 No Hit CTCACCAAGAACTGGATTTGAAGGGGGAGTTCCCACGTGGTTACTGTGGT 5 0.125 No Hit GGCAGATACTCAAGTCAATGCACTTGAGGTTCTCGGTACCGAAAACGTTG 5 0.125 No Hit GGATTCTCTGGTGACTTTCTAACGCTTCCTTGTCCTGGTGAGGCATTTCC 5 0.125 No Hit TCCAGACTTTACCAATCCCGGATCACAAAAAACACAAGCATAAATACTTC 5 0.125 No Hit ACGCCATAAGCATTGAAGCTAGCACCCGTGACCTTATCAACCAGGACCCG 5 0.125 No Hit TGATGGGGTTGCAGCAATTGTCTGGTGGAGTGGTTGTGGTGGCAAGGAAG 5 0.125 No Hit GGCTCATCATGTGAGGAAGGAGAAACAAAAGCAGTGATTTTAAGAGAAGG 5 0.125 No Hit TTAACAATTGCCAGTTACCACCAACAGAACCACAAATACTAGCATCATCA 5 0.125 No Hit GGTTTGGCAGGGAATGGGGCAGCTGGTTTTTAGGGCCAGGAAAACCTTGC 5 0.125 No Hit CTTTAACAAGATTTATCAGTTCAACTCCAATGGCACATTCTTCTTCTTGC 5 0.125 No Hit TCCCAATTCTCCATCTTCTTTGCTTGAAAATTGCTTATATTGCTCTTGTC 5 0.125 No Hit GATCTCTACGCTTTCATATGCCTGTTTGTACATGTCCACCAAGCGAGAGC 5 0.125 No Hit GCGGTATTCATCACTCGCTTTGGCAATCTCAAACTGGTCTCTGAATTCAA 5 0.125 No Hit AATTGGATGCAATCATGCAATTAGGAGAACTTGCGACTAGTTTACTCGAA 5 0.125 No Hit GTCGTACATAGTACCCGCCTGGTAGAGACTAAACTACAAACTCGCCCTTG 5 0.125 No Hit GTATGATATTCCACCGGCAGACATGCCCAGCATTTTTGCCGTCAAACTGC 5 0.125 No Hit GGCGAAGGAAAGAAAAGGTGAGAATTTGTCTATGTTGTGTATTCAGCATT 5 0.125 No Hit CCATCTTTTTCACCTCCAATTTGTCTTCCTTGGCTCTGTGGGTCTTTGGA 5 0.125 No Hit CGGTTATGAATTGAAGCAGAACAAATTCTTAGCTGTGTGATTGCCACTCG 5 0.125 No Hit GGCAAGAACTCGTAGACCAGCATCGTCTCATCACTTTGAATACAGCATCC 5 0.125 No Hit ACCAGGAGTAACCTTGAAGGGCCAGTGCTTAATATCACCCTGGACTGAAG 5 0.125 No Hit GGATGTTGTTGTTGTTGCATCTGTTGGTGATGCTGCTGTTGCATTTGTTG 5 0.125 No Hit GCCTCATGTCCGATCCTGTAGTTCTCTCTGCACCAGCCCTCATGCTTGGT 5 0.125 No Hit GTTTTGCCCTTGTACTTGTGTGTGGTATCATCATTGCAACATCCTACGAG 5 0.125 No Hit TGGAAATGAAGCCATTTGTATATGCAGGGCCAGCAGTAGAGGTGACCACA 5 0.125 No Hit GGAGGCTTTGGTGGCTTAATAGGAGGCTTTGGTGGCTTTATAGGAGGAGT 5 0.125 No Hit GTTGTGTTTTGATGTAGTGCTGTTCGGTTTAGTGGACGGAGTGTTTATGA 5 0.125 No Hit CCTTATTTCTCGACTAACAGCCTCTCTCTGAGTAATAAGCTGGCTGGCAT 5 0.125 No Hit GCCATTATAAGCCTCCGAAACTATGAGCTGGTGACCATTATATTTTGGTC 5 0.125 No Hit GGGGTGAGGGCGATCCGGTGTTGGGTGGTGTGGACATGGGTGCTGGAGAA 5 0.125 No Hit ATCGTCTTTTCGGATAGGCATGGATCTCACGTTGTATTTCTGACGGAGGT 5 0.125 No Hit GTGGAAGGGAAGCCCAATTAGTTGAGGGCGGTGGCAATGACCTGGTTAGC 5 0.125 No Hit CTTCCTTCTTTGCTGTCCACACACAGTAAGTCCCAGGTTCTGCACTGTCT 5 0.125 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0125 0.0 0.0 0.0 0.0 58-59 0.025 0.0 0.0 0.0 0.0 60-61 0.025 0.0 0.0 0.0 0.0 62-63 0.075 0.0 0.0 0.0 0.0 64-65 0.1 0.0 0.0 0.0 0.0 66-67 0.125 0.0 0.0 0.0 0.0 68-69 0.125 0.0 0.0 0.0 0.0 70-71 0.125 0.0 0.0 0.0 0.0 72-73 0.15 0.0 0.0 0.0 0.0 74-75 0.175 0.0 0.0 0.0 0.0 76-77 0.1875 0.0 0.0 0.0 0.0 78-79 0.25 0.0 0.0 0.0 0.0 80-81 0.2875 0.0 0.0 0.0 0.0 82-83 0.4 0.0 0.0 0.0 0.0 84-85 0.425 0.0 0.0 0.0 0.0 86-87 0.475 0.0 0.0 0.0 0.0 88-89 0.5625 0.0 0.0 0.0 0.0 90-91 0.6125 0.0 0.0 0.0 0.0 92-93 0.75 0.0 0.0 0.0 0.0 94-95 1.0750000000000002 0.0 0.0 0.0 0.0 96-97 1.4 0.0 0.0 0.0 0.0 98-99 1.675 0.0 0.0 0.0 0.0 100-101 2.0875 0.0 0.0 0.0 0.0 102-103 2.45 0.0 0.0 0.0 0.0 104-105 2.9625 0.0 0.0 0.0 0.0 106-107 3.1500000000000004 0.0 0.0 0.0 0.0 108-109 3.5250000000000004 0.0 0.0 0.0 0.0 110-111 3.8375 0.0 0.0 0.0 0.0 112-113 4.275 0.0 0.0 0.0 0.0 114-115 4.7125 0.0 0.0 0.0 0.0 116-117 5.2625 0.0 0.0 0.0 0.0 118-119 5.737500000000001 0.0 0.0 0.0 0.0 120-121 6.225 0.0 0.0 0.0 0.0 122-123 6.7375 0.0 0.0 0.0 0.0 124-125 7.300000000000001 0.0 0.0 0.0 0.0 126-127 7.85 0.0 0.0 0.0 0.0 128-129 8.537500000000001 0.0 0.0 0.0 0.0 130-131 9.025 0.0 0.0 0.0 0.0 132-133 9.625 0.0 0.0 0.0 0.0 134-135 10.162500000000001 0.0 0.0 0.0 0.0 136-137 11.2 0.0 0.0 0.0 0.0 138-139 12.05 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATAATAC 10 0.006830828 145.0 145 >>END_MODULE SRR26075390 read2 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR26075390_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.2015 37.0 37.0 37.0 37.0 37.0 2 36.338 37.0 37.0 37.0 37.0 37.0 3 36.2255 37.0 37.0 37.0 37.0 37.0 4 36.28 37.0 37.0 37.0 37.0 37.0 5 36.244 37.0 37.0 37.0 37.0 37.0 6 36.218 37.0 37.0 37.0 37.0 37.0 7 36.1905 37.0 37.0 37.0 37.0 37.0 8 36.223 37.0 37.0 37.0 37.0 37.0 9 36.2485 37.0 37.0 37.0 37.0 37.0 10-14 36.192099999999996 37.0 37.0 37.0 37.0 37.0 15-19 36.1059 37.0 37.0 37.0 37.0 37.0 20-24 36.059799999999996 37.0 37.0 37.0 37.0 37.0 25-29 35.9882 37.0 37.0 37.0 37.0 37.0 30-34 35.855900000000005 37.0 37.0 37.0 37.0 37.0 35-39 35.8388 37.0 37.0 37.0 37.0 37.0 40-44 35.780199999999994 37.0 37.0 37.0 37.0 37.0 45-49 35.7556 37.0 37.0 37.0 37.0 37.0 50-54 35.6115 37.0 37.0 37.0 37.0 37.0 55-59 35.6429 37.0 37.0 37.0 37.0 37.0 60-64 35.702000000000005 37.0 37.0 37.0 37.0 37.0 65-69 35.586 37.0 37.0 37.0 37.0 37.0 70-74 35.587999999999994 37.0 37.0 37.0 37.0 37.0 75-79 35.5101 37.0 37.0 37.0 37.0 37.0 80-84 35.5056 37.0 37.0 37.0 37.0 37.0 85-89 35.5443 37.0 37.0 37.0 37.0 37.0 90-94 35.4392 37.0 37.0 37.0 37.0 37.0 95-99 35.4379 37.0 37.0 37.0 37.0 37.0 100-104 35.3871 37.0 37.0 37.0 37.0 37.0 105-109 35.3808 37.0 37.0 37.0 34.6 37.0 110-114 35.3172 37.0 37.0 37.0 37.0 37.0 115-119 35.318200000000004 37.0 37.0 37.0 37.0 37.0 120-124 35.118700000000004 37.0 37.0 37.0 29.8 37.0 125-129 35.17280000000001 37.0 37.0 37.0 32.2 37.0 130-134 35.0844 37.0 37.0 37.0 32.2 37.0 135-139 35.047399999999996 37.0 37.0 37.0 29.8 37.0 140-144 34.9544 37.0 37.0 37.0 25.0 37.0 145-149 34.974599999999995 37.0 37.0 37.0 25.0 37.0 150-151 34.661249999999995 37.0 37.0 37.0 25.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.0 1101 2 0.0 1101 3 0.0 1101 4 0.0 1101 5 0.0 1101 6 0.0 1101 7 0.0 1101 8 0.0 1101 9 0.0 1101 10-14 0.0 1101 15-19 0.0 1101 20-24 0.0 1101 25-29 0.0 1101 30-34 0.0 1101 35-39 0.0 1101 40-44 0.0 1101 45-49 0.0 1101 50-54 0.0 1101 55-59 0.0 1101 60-64 0.0 1101 65-69 0.0 1101 70-74 0.0 1101 75-79 0.0 1101 80-84 0.0 1101 85-89 0.0 1101 90-94 0.0 1101 95-99 0.0 1101 100-104 0.0 1101 105-109 0.0 1101 110-114 0.0 1101 115-119 0.0 1101 120-124 0.0 1101 125-129 0.0 1101 130-134 0.0 1101 135-139 0.0 1101 140-144 0.0 1101 145-149 0.0 1101 150-151 0.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 12 1.0 13 9.0 14 13.0 15 10.0 16 5.0 17 5.0 18 12.0 19 9.0 20 7.0 21 13.0 22 14.0 23 10.0 24 9.0 25 17.0 26 12.0 27 9.0 28 7.0 29 10.0 30 24.0 31 31.0 32 39.0 33 83.0 34 164.0 35 566.0 36 2665.0 37 256.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 48.449999999999996 22.7 7.9 20.95 2 28.925 26.474999999999998 27.450000000000003 17.150000000000002 3 23.974999999999998 25.75 30.349999999999998 19.925 4 26.325 34.2 21.7 17.775 5 27.650000000000002 36.9 19.2 16.25 6 22.0 38.525 21.725 17.75 7 23.625 22.85 34.2 19.325 8 21.375 26.55 25.900000000000002 26.174999999999997 9 24.325 24.95 27.400000000000002 23.325000000000003 10-14 25.924999999999997 28.735 24.45 20.89 15-19 24.925 28.435 26.355 20.285 20-24 24.485 29.32 25.245 20.95 25-29 25.755 27.465 26.765 20.015 30-34 24.315 28.685 26.685 20.315 35-39 25.525 27.36 26.665 20.45 40-44 24.97 28.87 25.865 20.294999999999998 45-49 24.725 29.275000000000002 26.340000000000003 19.66 50-54 23.96 28.48 27.284999999999997 20.275000000000002 55-59 24.54 27.925 26.995 20.54 60-64 24.654999999999998 28.12 26.22 21.005 65-69 25.419999999999998 28.945 25.555 20.080000000000002 70-74 24.87 29.435 25.405 20.29 75-79 24.09 29.709999999999997 26.090000000000003 20.11 80-84 23.5 28.76 27.505000000000003 20.235 85-89 25.4 29.125 25.569999999999997 19.905 90-94 24.485 28.849999999999998 26.095000000000002 20.57 95-99 24.375 29.794999999999998 25.374999999999996 20.455000000000002 100-104 25.36 29.060000000000002 25.5 20.080000000000002 105-109 24.38 29.060000000000002 25.61 20.95 110-114 24.735 28.994999999999997 25.66 20.61 115-119 25.575 28.64 26.005 19.78 120-124 25.7 28.994999999999997 25.66 19.645000000000003 125-129 25.255 28.494999999999997 26.009999999999998 20.24 130-134 25.445 28.749999999999996 25.919999999999998 19.885 135-139 25.595000000000002 29.145 26.075 19.185 140-144 25.465 29.375 25.650000000000002 19.509999999999998 145-149 25.95 29.59 26.44 18.02 150-151 26.450000000000003 27.787499999999998 25.5 20.2625 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 1.5 9 2.0 10 2.0 11 2.5 12 2.0 13 1.0 14 1.0 15 2.0 16 2.0 17 1.0 18 2.0 19 3.5 20 2.5 21 1.0 22 1.5 23 3.0 24 3.5 25 2.5 26 2.0 27 2.0 28 5.0 29 10.5 30 13.0 31 14.5 32 11.5 33 14.5 34 28.5 35 43.5 36 61.0 37 97.5 38 139.5 39 149.5 40 167.5 41 214.5 42 231.0 43 240.0 44 273.0 45 296.5 46 296.0 47 246.0 48 228.5 49 222.5 50 194.5 51 163.0 52 118.5 53 87.5 54 58.5 55 56.5 56 47.0 57 34.0 58 28.5 59 19.5 60 19.0 61 20.0 62 16.0 63 10.0 64 7.0 65 4.0 66 3.0 67 5.0 68 3.0 69 3.0 70 4.5 71 3.0 72 2.5 73 1.5 74 0.5 75 0.0 76 0.5 77 1.5 78 2.0 79 2.0 80 2.5 81 1.5 82 1.0 83 1.0 84 0.0 85 1.5 86 3.0 87 2.0 88 1.0 89 1.0 90 1.0 91 1.0 92 0.5 93 1.0 94 2.5 95 2.0 96 1.0 97 1.5 98 2.0 99 1.0 100 6.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150-151 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 61.85000000000001 #Duplication Level Percentage of deduplicated Percentage of total 1 64.3088116410671 39.775 2 20.89733225545675 25.85 3 9.013742926434922 16.725 4 3.3144704931285367 8.200000000000001 5 1.2934518997574778 4.0 6 0.6871463217461601 2.55 7 0.16168148746968472 0.7000000000000001 8 0.16168148746968472 0.8 9 0.0 0.0 >10 0.16168148746968472 1.4000000000000001 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG 26 0.65 No Hit GAAGGAGCCACTCAAGGAGCACAAGAGGTATAACTTGAAGAAGCTAACCT 10 0.25 No Hit GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGT 10 0.25 No Hit TGAAAGAACTGAGCAAGGGCTATAGCATAGTTGGTCTTTCCCAGGGCAAC 10 0.25 No Hit TCCAGATTATCCATTTAAGCCTCCCAAGGTAGCATTCCGAACAAAGGTAT 8 0.2 No Hit GACAACAACTACTACAGAAACATATTGGACAACAAGGGCTTGTTGATAGT 8 0.2 No Hit AGCACATCGCGTTGCTCAACACTATGGGCTGATAACCATGGTACAGGATA 8 0.2 No Hit GTGCAGGCCCTGCTGGTAGTGACATGTTCCATTGGCAAGCAACAATTATG 8 0.2 No Hit GTGACAGATGTGGAGCGAACTCTGAAGGAGTTACAGGCCAAACCAGAATT 7 0.17500000000000002 No Hit CCTTAAACTACGCCGTATTTGCGGGAGCGCGCCGTTTCTTTGGCTAAATT 7 0.17500000000000002 No Hit CTCTGAAACATCGAGTGCAACCATGCCACTCTTTCAGGTCACAGCTGTCA 7 0.17500000000000002 No Hit AACAGAAGGAAAATCAGGTGACCAAAAATGAAAGAAGCAGACCGAAGACA 7 0.17500000000000002 No Hit CACTACTTTGAAGATTTTCAAAAATGGACTCTCATGGGGACTTCAAGAAC 6 0.15 No Hit GGTTGATCGGTGAAGCAGCCAAGAATCTGGCGGCTGTCAATCCCGAGAGG 6 0.15 No Hit CAGAGACAGCTATGGCTGCCTCTGGAGCCACCAAGGGTGCTTATGTCCCA 6 0.15 No Hit GTTAGCGGATATTGTGGTTGCTTAATCCAGGATGCAGGCCAGCGATAGAT 6 0.15 No Hit GCAGGACGTGGTCCTTGTTTCAACTACCAGGGAGGGCTTTTAGGGTGATA 6 0.15 No Hit CACCATGCCATACTTGAGGGTACTCTCTTGAAGCCGAACATGGTTACTCC 6 0.15 No Hit TTTGTGAAAGTGATCCCTCAACGGTGACTAATTGCAAGCATGAGTTCCAT 6 0.15 No Hit ATCACCGTGGTCTCCGTCACTATTGGGGTCTTCGAGTGCGAGGACAGCAC 6 0.15 No Hit GTTGTTAGGAATAAGGTCCAGGCTGAGTTTCGCTGGTGGGATAGAGTTCA 6 0.15 No Hit TCGAGAAATGGCAGCTGAAGATGGACAAGTGATCGGGTGCCACACTGTTG 6 0.15 No Hit CCTCTCTATACTACATCGACTATATTGCTACCCTTCACAAGGTTGACAAG 6 0.15 No Hit ACCATCAATCCAACTCGCAAGAACCTCAACCACATTTGCCACAAAATTAC 6 0.15 No Hit GAATGAGTTGGTGGAATCTGCAGCAGAGATGCTTTACGGTCTTATTCATG 6 0.15 No Hit AACATCTTTGAAGCTGTGTTCTCTGCTGAGGCATTTCTTTTTCCCAAACA 6 0.15 No Hit GATTACTAATGTTACACGTACAAAACTAAAGATTCTCAAGGTGGAAGTCC 6 0.15 No Hit GACTCAAGTTCTGAGGAAGGAAGCTGTCAAGAATGGGCTTGAACTGGTAC 6 0.15 No Hit GCTGTGTTAGTTGCATTGAAATTTCTGTGGATGGGAAGAGTGCTTTGATT 6 0.15 No Hit CTTAGCTCCTCCTTCCCCCAGATCTCTCATTCGCTAACGTGCGCCAACTC 5 0.125 No Hit GTCTGTGAAGCGCGAGTTCTTCACCGGTACCGACCTCCATACCCACTGCC 5 0.125 No Hit AAATAAATAAAAACAGAGACAGTGTTAAAATTCCATTCCAATTCGAATGG 5 0.125 No Hit TTGACAATGTTAAGGCCAAAATCCAGGACAAGGAGGGGATTCCACCAGAC 5 0.125 No Hit CTCAGGTGAAGGGTAAGGATGGAAGAGATGCTGTGGCTCGAACTGCTGTT 5 0.125 No Hit CAGTGATTTTGATGACCACTTGGAGCTAGCACTCAAAACATACCAAGAAT 5 0.125 No Hit GCCAAACTGGAAATCTGTTTCTGAGACAGTCATAGACTCTGATGATAGCC 5 0.125 No Hit TTTGGTTTCTCTGAAATTCCAAGAAAGCCTTTTCATGGCAGCAAAACAGG 5 0.125 No Hit AAAAGAAACACGAAATGGCATCTTCTGTCAAGATTTCTATGGTGGTGGTG 5 0.125 No Hit CCAAAAGCAGAGAAAGTCTCCATCTTTGATTGAGAAACCCTAAACCTAAA 5 0.125 No Hit CTTGATTCTGCCAAGCAGCTGTGGAAAAACAGGAAGGAATTGAAGGTTGA 5 0.125 No Hit GCAAAGTCTCTTGTCATTCGAATTCTGATCCTTCAACACTACCACCATCG 5 0.125 No Hit AGGCAAGAGGTTAGCAGAGTTGTAGGGGTAGCAAAAGAGAGTGATTTTTG 5 0.125 No Hit GTCCAAGCTAAGCATGCTACATATGTCCCACACACAGCTGGGAGATACTC 5 0.125 No Hit GGATGTCCTCATCAAGTGAGGAATAGACAGTCATGCATAAAAGTGGCCCT 5 0.125 No Hit AGTACACCGAGGAAGACGACAAGGCCATTGACGACTGGATCGCTGACCAC 5 0.125 No Hit GACAACTCCAAGAGAAGAAGCTTTGTGCAGTAATCCAGTTACCATCTCAG 5 0.125 No Hit CAGGAAGTCGCGATCTCCAAATGGTGAAGATTGGGCTTCGAGTTCTGACT 5 0.125 No Hit GCAATGCCAAGGACTGACAGTGAAGGATTTCTCTGTGGGGCAAATTTCAA 5 0.125 No Hit CAGGAAAGGCAGCTAAGGATCCAAACAAGCCTAAGAGGCCTGCTAGCGCA 5 0.125 No Hit AGTAAAGTAAGGAGACACGATGGCTAAGTTTGCTGTGGCTAATCTCTTGA 5 0.125 No Hit CAGCAGCAGCCATCCACTAAGACACAACAAAGCCAACAAGGCCATCAAAA 5 0.125 No Hit ATTTGATACCAGCCAATTTGTCAGGCTTGGGAACCGTAGATTTGGTAGCT 5 0.125 No Hit ACAAGACGCCCTCGCAGTAAACCCTCCTGTCTCTCTCCCTCTCGAAATCT 5 0.125 No Hit GAACAAAGATATCCGGGCATGACTAAATGTTTCTGCTCATGGATTTCTTA 5 0.125 No Hit GTGAGATCCATGGCACGGTTGGAATCTACATATCCGTGGGTGATGGACCA 5 0.125 No Hit GCCCAAACCCTCCAGAACCAAGTTAGCCAACTCTATAACGAGGCTCAGAA 5 0.125 No Hit GACAAGAAATAGCTGTCAAGAGGCTCTCTAAGAATTCAAGACAAGGACTT 5 0.125 No Hit GTCTCTTCCCTCAATTCCTACCTTCCATTTCTTCTTCTTCATCATCTTCT 5 0.125 No Hit GAAAATTCAGAATGATTGAAGAACTGCGAACATTGTCTCTTGGATCCACC 5 0.125 No Hit CTTGCAATTACTTCTCAATATGAACAATAACAACTCTATTGGTACTGCTT 5 0.125 No Hit CACAATTCTCTCCAATTTCAGCTGATGATATCTTTTACAACGGCCAAATC 5 0.125 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0125 0.0 0.0 0.0 0.0 58-59 0.025 0.0 0.0 0.0 0.0 60-61 0.025 0.0 0.0 0.0 0.0 62-63 0.075 0.0 0.0 0.0 0.0 64-65 0.1 0.0 0.0 0.0 0.0 66-67 0.125 0.0 0.0 0.0 0.0 68-69 0.125 0.0 0.0 0.0 0.0 70-71 0.125 0.0 0.0 0.0 0.0 72-73 0.15 0.0 0.0 0.0 0.0 74-75 0.175 0.0 0.0 0.0 0.0 76-77 0.1875 0.0 0.0 0.0 0.0 78-79 0.25 0.0 0.0 0.0 0.0 80-81 0.30000000000000004 0.0 0.0 0.0 0.0 82-83 0.42500000000000004 0.0 0.0 0.0 0.0 84-85 0.45 0.0 0.0 0.0 0.0 86-87 0.5 0.0 0.0 0.0 0.0 88-89 0.5874999999999999 0.0 0.0 0.0 0.0 90-91 0.6375 0.0 0.0 0.0 0.0 92-93 0.775 0.0 0.0 0.0 0.0 94-95 1.1 0.0 0.0 0.0 0.0 96-97 1.4249999999999998 0.0 0.0 0.0 0.0 98-99 1.7 0.0 0.0 0.0 0.0 100-101 2.0875 0.0 0.0 0.0 0.0 102-103 2.4375 0.0 0.0 0.0 0.0 104-105 2.9625 0.0 0.0 0.0 0.0 106-107 3.1500000000000004 0.0 0.0 0.0 0.0 108-109 3.5250000000000004 0.0 0.0 0.0 0.0 110-111 3.8375 0.0 0.0 0.0 0.0 112-113 4.275 0.0 0.0 0.0 0.0 114-115 4.7375 0.0 0.0 0.0 0.0 116-117 5.275 0.0 0.0 0.0 0.0 118-119 5.8375 0.0 0.0 0.0 0.0 120-121 6.324999999999999 0.0 0.0 0.0 0.0 122-123 6.8875 0.0 0.0 0.0 0.0 124-125 7.4875 0.0 0.0 0.0 0.0 126-127 8.1 0.0 0.0 0.0 0.0 128-129 8.8125 0.0 0.0 0.0 0.0 130-131 9.275 0.0 0.0 0.0 0.0 132-133 9.875 0.0 0.0 0.0 0.0 134-135 10.4375 0.0 0.0 0.0 0.0 136-137 11.425 0.0 0.0 0.0 0.0 138-139 12.1875 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content pass >>END_MODULE Read 2231969 spots for SRR26075390.sra Written 2231969 spots for SRR26075390.sra Read 2231969 spots for SRR26075390.sra Written 2231969 spots for SRR26075390.sra Read 2231969 spots for SRR26075390.sra Written 2231969 spots for SRR26075390.sra Read 2231969 spots for SRR26075390.sra Written 2231969 spots for SRR26075390.sra Read 2231969 spots for SRR26075390.sra Written 2231969 spots for SRR26075390.sra Read 2231969 spots for SRR26075390.sra Written 2231969 spots for SRR26075390.sra Read 2231969 spots for SRR26075390.sra Written 2231969 spots for SRR26075390.sra Read 2231969 spots for SRR26075390.sra Written 2231969 spots for SRR26075390.sra Read 2231969 spots for SRR26075390.sra Written 2231969 spots for SRR26075390.sra Read 2231969 spots for SRR26075390.sra Written 2231969 spots for SRR26075390.sra Read 2231977 spots for SRR26075390.sra Written 2231977 spots for SRR26075390.sra Read 2231969 spots for SRR26075390.sra Written 2231969 spots for SRR26075390.sra Read 2231969 spots for SRR26075390.sra Written 2231969 spots for SRR26075390.sra Read 2231969 spots for SRR26075390.sra Written 2231969 spots for SRR26075390.sra Read 2231969 spots for SRR26075390.sra Written 2231969 spots for SRR26075390.sra Read 2231969 spots for SRR26075390.sra Written 2231969 spots for SRR26075390.sra Read 2231969 spots for SRR26075390.sra Written 2231969 spots for SRR26075390.sra Read 2231969 spots for SRR26075390.sra Written 2231969 spots for SRR26075390.sra Read 2231969 spots for SRR26075390.sra Written 2231969 spots for SRR26075390.sra Read 2231969 spots for SRR26075390.sra Written 2231969 spots for SRR26075390.sra SRR ids: ['SRR26075390.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_l714ytzg SRR26075390.sra spots: 44639388 blocks: [[1, 2231969], [2231970, 4463938], [4463939, 6695907], [6695908, 8927876], [8927877, 11159845], [11159846, 13391814], [13391815, 15623783], [15623784, 17855752], [17855753, 20087721], [20087722, 22319690], [22319691, 24551659], [24551660, 26783628], [26783629, 29015597], [29015598, 31247566], [31247567, 33479535], [33479536, 35711504], [35711505, 37943473], [37943474, 40175442], [40175443, 42407411], [42407412, 44639388]] SRR26075390 file size 16487650 SRR26075390 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o SRR26075390 SRR26075390_1.fastq SRR26075390_2.fastq Input file: SRR26075390_1.fastq Paired file: SRR26075390_2.fastq trimmed: SRR26075390-trimmed-pair1.fastq, SRR26075390-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Wed Feb 12 00:18:30 2025 >> started Wed Feb 12 00:19:19 2025 >> done (49.048s) 44639388 read pairs processed; of these: 323 ( 0.00%) short read pairs filtered out after trimming by size control 110111 ( 0.25%) empty read pairs filtered out after trimming by size control 44528954 (99.75%) read pairs available; of these: 7164713 (16.09%) trimmed read pairs available after processing 37364241 (83.91%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 18 38 0.00% 19 36 0.00% 20 56 0.00% 21 31 0.00% 22 51 0.00% 23 88 0.00% 24 59 0.00% 25 73 0.00% 26 85 0.00% 27 92 0.00% 28 117 0.00% 29 99 0.00% 30 121 0.00% 31 127 0.00% 32 108 0.00% 33 145 0.00% 34 123 0.00% 35 155 0.00% 36 165 0.00% 37 202 0.00% 38 211 0.00% 39 234 0.00% 40 221 0.00% 41 251 0.00% 42 266 0.00% 43 303 0.00% 44 364 0.00% 45 347 0.00% 46 406 0.00% 47 421 0.00% 48 515 0.00% 49 578 0.00% 50 630 0.00% 51 732 0.00% 52 773 0.00% 53 877 0.00% 54 873 0.00% 55 1075 0.00% 56 1127 0.00% 57 1256 0.00% 58 1499 0.00% 59 1707 0.00% 60 2060 0.00% 61 2286 0.01% 62 2475 0.01% 63 2550 0.01% 64 3040 0.01% 65 3341 0.01% 66 3508 0.01% 67 4025 0.01% 68 4579 0.01% 69 5069 0.01% 70 5944 0.01% 71 6538 0.01% 72 7634 0.02% 73 8775 0.02% 74 9470 0.02% 75 10670 0.02% 76 11613 0.03% 77 12630 0.03% 78 13824 0.03% 79 14977 0.03% 80 16986 0.04% 81 18585 0.04% 82 20755 0.05% 83 22596 0.05% 84 24874 0.06% 85 27386 0.06% 86 28765 0.06% 87 30470 0.07% 88 31859 0.07% 89 33986 0.08% 90 36741 0.08% 91 38955 0.09% 92 41264 0.09% 93 45586 0.10% 94 47612 0.11% 95 49702 0.11% 96 52538 0.12% 97 54042 0.12% 98 56456 0.13% 99 58066 0.13% 100 60465 0.14% 101 62689 0.14% 102 65923 0.15% 103 69114 0.16% 104 72633 0.16% 105 75659 0.17% 106 78940 0.18% 107 80634 0.18% 108 81378 0.18% 109 84259 0.19% 110 84281 0.19% 111 87762 0.20% 112 90609 0.20% 113 93289 0.21% 114 96025 0.22% 115 100615 0.23% 116 102751 0.23% 117 104193 0.23% 118 107246 0.24% 119 107467 0.24% 120 108822 0.24% 121 112133 0.25% 122 114458 0.26% 123 117111 0.26% 124 122647 0.28% 125 122469 0.28% 126 127383 0.29% 127 129984 0.29% 128 132338 0.30% 129 133883 0.30% 130 135580 0.30% 131 137316 0.31% 132 141270 0.32% 133 143431 0.32% 134 145398 0.33% 135 148984 0.33% 136 151740 0.34% 137 152733 0.34% 138 156864 0.35% 139 157580 0.35% 140 159938 0.36% 141 162875 0.37% 142 163781 0.37% 143 164441 0.37% 144 170365 0.38% 145 172973 0.39% 146 172693 0.39% 147 175100 0.39% 148 177560 0.40% 149 177336 0.40% 150 180726 0.41% 151 37364241 83.91% 44528954 reads passed initial QC criterion=sequence-density sequence-density=0.36 sequence-density-rank=1 fanout-score=3.30 fanout-score-rank=31 prefix-density=0.38 prefix-fanout=3.1 sequence=CCACACTTGCAG criterion=fanout-score sequence-density=0.08 sequence-density-rank=16 fanout-score=170.73 fanout-score-rank=1 prefix-density=0.59 prefix-fanout=23.6 sequence=TCATCTTCATCA criterion=sequence-density sequence-density=0.23 sequence-density-rank=1 fanout-score=11.83 fanout-score-rank=15 prefix-density=0.67 prefix-fanout=4.0 sequence=TGGCTGCAAGTG criterion=fanout-score sequence-density=0.10 sequence-density-rank=12 fanout-score=385.46 fanout-score-rank=1 prefix-density=1.10 prefix-fanout=34.4 sequence=AAGAAGAAGAAA SRR26075390 testing PE reads STAR mapping to Ensembl genome Started job on | Feb 12 00:19:59 Started mapping on | Feb 12 00:20:00 Finished on | Feb 12 00:27:00 Mapping speed, Million of reads per hour | 381.68 Number of input reads | 44528954 Average input read length | 293 UNIQUE READS: Uniquely mapped reads number | 39521553 Uniquely mapped reads % | 88.75% Average mapped length | 291.64 Number of splices: Total | 37717280 Number of splices: Annotated (sjdb) | 36816477 Number of splices: GT/AG | 37050197 Number of splices: GC/AG | 512527 Number of splices: AT/AC | 40622 Number of splices: Non-canonical | 113934 Mismatch rate per base, % | 0.39% Deletion rate per base | 0.03% Deletion average length | 2.92 Insertion rate per base | 0.02% Insertion average length | 2.51 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 981084 % of reads mapped to multiple loci | 2.20% Number of reads mapped to too many loci | 135713 % of reads mapped to too many loci | 0.30% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 8.25% % of reads unmapped: other | 0.48% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 4026317 4026317 4026317 N_multimapping 981084 981084 981084 N_noFeature 1110652 39114457 1332807 N_ambiguous 390832 2694 203951 UnstrandedReadsAssigned:38020069 PositiveStrandReadsAssigned:404402 NegativeStrandReadsAssigned:37984795 Dataset is classified negative stranded MeadianReadLen=151 20thPercentileLength=151 echo kmer=147 SRR26075390 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,400 [index] number of k-mers: 62,057,036 [index] number of equivalence classes: 130,681 [quant] running in paired-end mode [quant] will process pair 1: SRR26075390-trimmed-pair1.fastq SRR26075390-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 44,528,954 reads, 38,594,995 reads pseudoaligned [quant] estimated average fragment length: 213.095 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,278 rounds 52401 SRR26075390.ke.tsv 34699 SRR26075390.se.tsv 87100 total ==> SRR26075390.ke.tsv <== target_id length eff_length est_counts tpm Potri.005G200100.1.v4.1 2018 1805.91 4300 59.4567 Potri.005G024800.1.v4.1 1035 822.905 7477 226.885 Potri.004G059700.1.v4.1 961 748.912 2 0.0666848 Potri.007G009000.2.v4.1 1416 1203.91 0 0 Potri.003G141000.2.v4.1 2943 2730.91 2140.16 19.5689 Potri.016G087400.1.v4.1 270 91.3536 4267 1166.34 Potri.015G069301.1.v4.1 564 354.271 0 0 Potri.010G195200.1.v4.1 1773 1560.91 1476 23.6123 Potri.012G127500.1.v4.1 977 764.912 81883 2673.07 ==> SRR26075390.se.tsv <== Potri.001G166300.v4.1 0 Potri.001G448400.v4.1 55 Potri.001G233950.v4.1 3 Potri.001G122700.v4.1 494 Potri.001G212900.v4.1 0 Potri.001G182400.v4.1 0 Potri.001G256600.v4.1 0 Potri.001G040500.v4.1 0 Potri.001G416900.v4.1 0 Potri.001G452600.v4.1 510 SRR26075390 completed mapping pipeline successfully