Starting /dee2/code/volunteer_pipeline.sh SRR26075391 current disk space = 3051554443264 free memory = 1446007288 SRR26075391 SRAfilesize c02e72e5a2af4239a071ed6937ea2a89 SRR26075391.sra SRR26075391.sra file validated SRR26075391 is paired end SRR26075391 is conventional basespace SRR26075391 read1 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR26075391_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.66675 37.0 37.0 37.0 37.0 37.0 2 36.572 37.0 37.0 37.0 37.0 37.0 3 36.6305 37.0 37.0 37.0 37.0 37.0 4 36.6375 37.0 37.0 37.0 37.0 37.0 5 36.66 37.0 37.0 37.0 37.0 37.0 6 36.6805 37.0 37.0 37.0 37.0 37.0 7 36.6115 37.0 37.0 37.0 37.0 37.0 8 36.648 37.0 37.0 37.0 37.0 37.0 9 36.6575 37.0 37.0 37.0 37.0 37.0 10-14 36.67009999999999 37.0 37.0 37.0 37.0 37.0 15-19 36.6473 37.0 37.0 37.0 37.0 37.0 20-24 36.5426 37.0 37.0 37.0 37.0 37.0 25-29 36.4772 37.0 37.0 37.0 37.0 37.0 30-34 36.419799999999995 37.0 37.0 37.0 37.0 37.0 35-39 36.3702 37.0 37.0 37.0 37.0 37.0 40-44 36.3606 37.0 37.0 37.0 37.0 37.0 45-49 36.3182 37.0 37.0 37.0 37.0 37.0 50-54 36.273199999999996 37.0 37.0 37.0 37.0 37.0 55-59 36.189499999999995 37.0 37.0 37.0 37.0 37.0 60-64 36.186800000000005 37.0 37.0 37.0 37.0 37.0 65-69 36.143600000000006 37.0 37.0 37.0 37.0 37.0 70-74 36.0963 37.0 37.0 37.0 37.0 37.0 75-79 36.153499999999994 37.0 37.0 37.0 37.0 37.0 80-84 36.0102 37.0 37.0 37.0 37.0 37.0 85-89 35.9504 37.0 37.0 37.0 37.0 37.0 90-94 35.9097 37.0 37.0 37.0 37.0 37.0 95-99 35.954899999999995 37.0 37.0 37.0 37.0 37.0 100-104 35.91080000000001 37.0 37.0 37.0 37.0 37.0 105-109 35.826800000000006 37.0 37.0 37.0 37.0 37.0 110-114 35.8106 37.0 37.0 37.0 37.0 37.0 115-119 35.6135 37.0 37.0 37.0 37.0 37.0 120-124 35.6203 37.0 37.0 37.0 37.0 37.0 125-129 35.4684 37.0 37.0 37.0 37.0 37.0 130-134 35.3257 37.0 37.0 37.0 34.6 37.0 135-139 35.2193 37.0 37.0 37.0 32.2 37.0 140-144 35.088800000000006 37.0 37.0 37.0 25.0 37.0 145-149 35.1579 37.0 37.0 37.0 29.8 37.0 150-151 34.985 37.0 37.0 37.0 25.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.0 1101 2 0.0 1101 3 0.0 1101 4 0.0 1101 5 0.0 1101 6 0.0 1101 7 0.0 1101 8 0.0 1101 9 0.0 1101 10-14 0.0 1101 15-19 0.0 1101 20-24 0.0 1101 25-29 0.0 1101 30-34 0.0 1101 35-39 0.0 1101 40-44 0.0 1101 45-49 0.0 1101 50-54 0.0 1101 55-59 0.0 1101 60-64 0.0 1101 65-69 0.0 1101 70-74 0.0 1101 75-79 0.0 1101 80-84 0.0 1101 85-89 0.0 1101 90-94 0.0 1101 95-99 0.0 1101 100-104 0.0 1101 105-109 0.0 1101 110-114 0.0 1101 115-119 0.0 1101 120-124 0.0 1101 125-129 0.0 1101 130-134 0.0 1101 135-139 0.0 1101 140-144 0.0 1101 145-149 0.0 1101 150-151 0.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 18 1.0 19 1.0 20 0.0 21 1.0 22 3.0 23 3.0 24 6.0 25 7.0 26 10.0 27 11.0 28 11.0 29 20.0 30 29.0 31 35.0 32 53.0 33 95.0 34 184.0 35 467.0 36 2884.0 37 179.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.30597948461346 14.335751813860394 8.131098323742806 36.22717037778334 2 20.775 12.875 34.275 32.074999999999996 3 16.125 17.424999999999997 29.2 37.25 4 21.425 25.424999999999997 26.1 27.05 5 24.075 31.175000000000004 23.549999999999997 21.2 6 20.349999999999998 35.65 23.225 20.775 7 15.275 29.599999999999998 39.35 15.775 8 16.3 25.900000000000002 33.75 24.05 9 17.075000000000003 25.275 33.5 24.15 10-14 19.791979197919794 29.57795779577958 27.25272527252725 23.377337733773377 15-19 19.705000000000002 27.815 28.125 24.355 20-24 20.19 27.38 28.23 24.2 25-29 19.814999999999998 28.410000000000004 27.650000000000002 24.125 30-34 20.0 28.095 27.765 24.14 35-39 20.369999999999997 27.310000000000002 27.855 24.465 40-44 21.08 26.895000000000003 27.644999999999996 24.38 45-49 20.28 28.494999999999997 27.33 23.895 50-54 21.465 27.82 27.715 23.0 55-59 20.715 27.169999999999998 27.894999999999996 24.22 60-64 19.655 27.705000000000002 28.225 24.415 65-69 19.465 28.34 27.925 24.27 70-74 21.085 26.795 28.09 24.03 75-79 20.89 27.79 27.315 24.005000000000003 80-84 20.830000000000002 26.895000000000003 28.249999999999996 24.025 85-89 20.61 28.275 27.73 23.385 90-94 20.855 27.11 27.96 24.075 95-99 21.64 27.415 27.47 23.474999999999998 100-104 20.765 27.334999999999997 27.37 24.529999999999998 105-109 21.62 28.110000000000003 26.740000000000002 23.53 110-114 21.415 27.33 27.215 24.04 115-119 20.435 28.325 26.945000000000004 24.295 120-124 20.985 27.845 26.735 24.435000000000002 125-129 20.93 27.705000000000002 27.495000000000005 23.87 130-134 21.349999999999998 27.365000000000002 27.0 24.285 135-139 21.555 28.035 26.334999999999997 24.075 140-144 21.39 27.700000000000003 26.375 24.535 145-149 22.21 27.485 26.75 23.555 150-151 22.162499999999998 26.437500000000004 26.55 24.85 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 1.0 15 1.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 3.5 24 5.0 25 3.5 26 3.5 27 3.0 28 6.5 29 12.0 30 10.5 31 13.0 32 21.0 33 26.5 34 35.0 35 58.5 36 80.5 37 104.0 38 135.0 39 157.0 40 201.5 41 223.5 42 221.0 43 248.0 44 274.5 45 279.0 46 267.0 47 243.5 48 215.0 49 202.5 50 190.0 51 157.5 52 127.5 53 106.5 54 84.0 55 64.5 56 48.0 57 33.5 58 24.0 59 17.5 60 13.0 61 15.0 62 16.0 63 9.0 64 8.5 65 9.5 66 6.0 67 5.5 68 3.0 69 0.5 70 1.5 71 1.0 72 0.0 73 0.0 74 0.5 75 0.5 76 0.0 77 0.0 78 0.0 79 0.5 80 0.5 81 0.0 82 0.0 83 0.0 84 0.5 85 0.5 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.075 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.01 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150-151 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 58.525000000000006 #Duplication Level Percentage of deduplicated Percentage of total 1 61.59760785988894 36.05 2 22.08457923964118 25.85 3 8.116189662537378 14.249999999999998 4 4.57069628363947 10.7 5 1.49508756941478 4.375 6 1.1106364801366937 3.9 7 0.3844510892780863 1.575 8 0.299017513882956 1.4000000000000001 9 0.21358393848782573 1.125 >10 0.12815036309269542 0.775 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCCATATTCGTCCCCCATAAGCATCATTGGTGTTCCCTGAGATATCATT 11 0.27499999999999997 No Hit GGTTAATATGACCTCTTTGAATCTGACGATGTTGGGATGCCTAAGCGACC 10 0.25 No Hit GACAATTGCTTTTGCTCCAGTTTACCCAACTCCGTGAAGAAATGGTCGAG 10 0.25 No Hit TATAACTATCTCCACTCCCAACACCTCTCTTATCCCTTAGTCGTCCGATA 9 0.22499999999999998 No Hit CTTTAAACCCAGCCAGCAATGAAGCACAGCTCCAACTTCCGCTGACAAGT 9 0.22499999999999998 No Hit GGCAAACTCCACAGCTGATAACCTTGATAAAAACATCTTCTGGCCCCGTA 9 0.22499999999999998 No Hit GTCAAAGTATCTAAAGTTCCTGAGGCCAAAGCTCCTGTCTGTCTCCTTCT 9 0.22499999999999998 No Hit CGCCCTTAGACGTCTTGGTATACGGACAACTGATGGACCCACGTTGCGAG 9 0.22499999999999998 No Hit GCATCCATACCTCCAATTGGAGCATTGTACTTTCAGCGATTCAAGAACCT 8 0.2 No Hit ATCATCAACCCCAGAGACTGAACTGGAAGGACAAAAATCAATGTCTTCTG 8 0.2 No Hit TGTTCATTCAACTCTTCTCTCCATGCCCTAAAGTCCATTTTCCCTTTAGC 8 0.2 No Hit ACCACCATTCATAAAGTAAAAACAACATTGAAAAACAACTGATCCTTTCC 8 0.2 No Hit GAATTTTCCAGTTCCATTGTAATACGCCGGATACTAGTCCCTGGCCTCAC 8 0.2 No Hit CTCATCTTCAGCTCCTTCAACATCATACTCACTGGAATCATTAACGACCA 8 0.2 No Hit CCTCTTCCATCTTTGTCTGCTCCCCTCCTTTAACTATAGGAACAGATTTT 8 0.2 No Hit GATCAACAATAATACAAATGATTCTAAAGCATATCCTGCCAGCCTAATAC 7 0.17500000000000002 No Hit CCCTTCGGTAGCTAACTCAACGGTGGATGATGCGGTGTTGGTAGAATCTT 7 0.17500000000000002 No Hit GTGCAGATGATGAGGATGGTGGTGGGGATGGTGGCGCAGGAGATAATGGG 7 0.17500000000000002 No Hit GCCTATTTTCAGCAACCAACTTCCTCAAATCACGGACTTTGATGGGAGAA 7 0.17500000000000002 No Hit CCGATTTTTCATTCAGTCGCCGATTGATTCATTGCTGGGGCGGTCTCTCC 7 0.17500000000000002 No Hit TGGCAAATCTTTCTCAACTTGCCCACTATGCAAACTGCATCGATGTCCCC 7 0.17500000000000002 No Hit GGTTAGTGACAGTAACATAAAACAGAACAGTGAACAGTAACAAATAGTAA 7 0.17500000000000002 No Hit CCTCAGTCTTCCTCCTCTCCCTCATTCTCGGCAATGTTAAAGTAGCGCAA 7 0.17500000000000002 No Hit GGTGACTGAAGTATCCGTGACTGAGGAGTGATCACTAGAAGAGGGTTGAG 7 0.17500000000000002 No Hit GCCATAACCGATGGGATGTTGTAATTCATTCCTCTTCCTAAAACAGGGAA 6 0.15 No Hit GGCAATTCTATAGTAGTGATTGAAGATATCGAAGCTAATGAAGTTCAGTT 6 0.15 No Hit AGTAGTAGTGTGGATGTTGTTGTTAGACACTTCTTTTTGTCTTTAAATAT 6 0.15 No Hit AACGAAATAACTAGCCGGCCCTTTACATTCTTTGAAAAAATCCTTATGAT 6 0.15 No Hit CCCTTATAAAAACAGCAAGTTTTTTCTTTCTTGTTGAAAAATAGCATGCA 6 0.15 No Hit CAACAATGTAAGATGCATACAGATCCATGGAATACTTGATGGCATCAATT 6 0.15 No Hit GTTAAACAAATAGCTATCAATGTCACTACACGTAGATTAACGTGATCTTG 6 0.15 No Hit TGGAGAGAACAGAAAAGGGCAGAGAAAAAAAGAGAGAGGGGTTGAAAATT 6 0.15 No Hit CGGCACTACAATTATCGTGTTCCCCACAAACTCCATGATCTCCAATCCAT 6 0.15 No Hit GGTCAGTTGACAGGTTATTGATACACTCAAGTATCTTCAAAAGAATAGGG 6 0.15 No Hit ACAGAACCACAACTAAAACTAAGCCATTAAAATCATAAAACAACCACGTG 6 0.15 No Hit AACGGAACAAGCTTTGTGCCATTCGGACCTACCGTAAGCCTATATTTCGT 6 0.15 No Hit AGATGGGTTTAAAACTCAAGAGATAAGGAAATGAATCAGCAAAAGACCTA 6 0.15 No Hit GGCGGCCACAAATGAAGGAGATGCTGTGAGCGGGGTCTGCTGGCCGGGGC 6 0.15 No Hit GTCAGCTTTAACCTTCATTCCACCAGTAATGCGAACTAGGGTTTCCCTCC 6 0.15 No Hit TGGTTTCGCTGGATAGTAGACAGGGACAGTGGGAATCTCGTTAATCCATT 6 0.15 No Hit AGTCTATCTAATAACTATGCCACCAACAACAGCAAAGAAACATACCAGGA 6 0.15 No Hit GCTCTCTAAACTCCTCCATGAAAACGAAAAATGCGCTAGCAGGCCTCTTA 6 0.15 No Hit CTCTAGTACCAGAGAAGATGGTTGGGGATCCAAAATTTCTGGTGCATTTT 6 0.15 No Hit TCCTATTACAAAGTAAATAAAATTAAATAGAAATTAAAGCATGTGTGTAA 6 0.15 No Hit TGTAGATCAGCAGCAACCAAGTGCTGCAATCATTTGCCCCAGTTTCCATA 6 0.15 No Hit GTTTATTTCATTAATAACTGGAGAGCAGGAGATGCCAGTGCCTCAGACAA 6 0.15 No Hit ATCAATTACAAAGCATCAGGCTAGTTGCAAAGAGTAGGTAATTACTAGCA 6 0.15 No Hit TGATCCTCCTCAAAGTCCCTGGACTGATATCTGAATCCGAACTCTTATCA 6 0.15 No Hit CAGAAGATGGGTTGTTAGGGTTAGGGTTAAGGGGTTTGATGGACAAGGAA 6 0.15 No Hit AGGAGATCAAAAAGTTACACGACACTATAAAGCAGGCACCTCCTTCCCTT 6 0.15 No Hit GACTTTTTAGCTTTCTCAGATGCCTCTTTTCGCTTATCCTTGTCAGAGCC 5 0.125 No Hit GCCAAAATAAGGGGGAGCAGAGCAGCAGCAATAGAAACGCACGCACTGGG 5 0.125 No Hit ACAACATACAAGACGTCCAATACATCTAAAAATCTGATCATTCTATAAGA 5 0.125 No Hit ACCAAATCATCAACTTCATATTCACAAAAGTCAGAAGAATCGATGGGCCA 5 0.125 No Hit CTTCTCAACCACTGATTCAATACCAGTGCTCACATACTCATCCCCATTGA 5 0.125 No Hit CGAGGGACCTCTCCCAGGGAAGGAACCCAGACGCAGAATCGGCAAAATTG 5 0.125 No Hit GCTTCACCATCATCATTGCCGTCCTCATCTTCTTCATACTCCACCCTTCT 5 0.125 No Hit GCCGAAGTAACCAGGATGGTATTTGTCGAAGAGGATCCTGTGGTGGTGCA 5 0.125 No Hit AGAAACTGTGTTGGTGATGAAGTTTCACAACTATGAAGCAGGCTCTGTGT 5 0.125 No Hit AGATAGTTTTGCGTTGATCTTTTCCATTTCATCAAGCTCCTTCATTAATG 5 0.125 No Hit GCCGCAGGCTCCACTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTT 5 0.125 No Hit CCCTCAAGTAAACCTTACCTGGGAAAAAAGAATTTTGTCAGTGATTCTAG 5 0.125 No Hit GCTCCATAGCTGCCTTGACAAGTTCTTCAATGGTTTGAGGAACCCACAGG 5 0.125 No Hit AGCCTGAATTCTCAGAACCTTTTGGAGCTCCTTAACTGGTGGAACCGATC 5 0.125 No Hit CCAGGAATACAAGCTGCCAGAAGTGCTCCCCGGAGTCCAGAACTGAACAT 5 0.125 No Hit CTCCGAGTATCATCTCCAGTTGCTCTGTATGACAACCAATCACTCAAGTC 5 0.125 No Hit GTCTCTTGAAACTAGCTCTGCTTCGACCTCCTTGGTGATGTTGTCCAATT 5 0.125 No Hit AGCAAAACGTAGCTGTCTTTCCAGTTCCTGATTGTGCTTGTTGGATTACA 5 0.125 No Hit CCTCCTCCTCTCATATTGCTACGGTTACCATCTGCCTCTGCTTCATCCTC 5 0.125 No Hit CTCCTCGTCAGAATTATCCACGGGCAACTCGTGCCTACAAACCGGACAAG 5 0.125 No Hit CTCCATAAAGAACATCCCATTCTTCTCCGCATAGTCAATGCCATCTTGAG 5 0.125 No Hit AGCAGGGAAAGTCTTCTTCTTGGCTAGATCACCAGAAGCACCAAGAACAA 5 0.125 No Hit AGAAGGGAATAGAGTGAGTGATGAATTTCTCCACTTCCAGCTCCTTATTC 5 0.125 No Hit CTGCAGCATACCATGTTGGCTTTGATTGAATTTCACCTGCTACAGCAATT 5 0.125 No Hit GGCCTGGAAAGCAGCATGTCTAAATTGCTCATATGAATGTGCATACATCA 5 0.125 No Hit GGAATGGAAACGCCTCGCTACCTCATTGGCACTATCCAGCAAGCAGTGCT 5 0.125 No Hit CTCCACTAGAGCCTTCAAAAGCTTAGCCACGGTAAGAGGAAACAAAGCAG 5 0.125 No Hit ACTCGGAAAAAAAATCAAGACCGAAACCACGCCACCGCCTATCAAGAACC 5 0.125 No Hit GTTCAGAGACAATTATGCACTCTCCTGTAGAGGCCGAATTGAGTATCTGT 5 0.125 No Hit CAACCATAAAAATTATGACAACCAGATACATTTCCTAGAAGAAAGCATTT 5 0.125 No Hit GTTGTGGGCAGTGAAAATTAACAACAAACACATGCTAGAAAACAAAAGGC 5 0.125 No Hit GTGCAAAAGAAAAACAACTAGCCACTATGTGATGAACAGATATCAATACA 5 0.125 No Hit CCGAAACCACGCCACCGCCTATCAAGAACCCAGTTCTGTGAACAAAAGCC 5 0.125 No Hit CGAGTTAACAGCCACAGACTCGGTGGGTCTGAGTTCAAGCATAGAAGCAT 5 0.125 No Hit TGTCCTTAGGCATGATGGTGACTCTCTTGGCATGAATAGCACACAAGTTG 5 0.125 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0 0.0 0.0 0.0 0.0 64-65 0.0 0.0 0.0 0.0 0.0 66-67 0.0 0.0 0.0 0.0 0.0 68-69 0.0 0.0 0.0 0.0 0.0 70-71 0.025 0.0 0.0 0.0 0.0 72-73 0.09999999999999999 0.0 0.0 0.0 0.0 74-75 0.175 0.0 0.0 0.0 0.0 76-77 0.275 0.0 0.0 0.0 0.0 78-79 0.3 0.0 0.0 0.0 0.0 80-81 0.3375 0.0 0.0 0.0 0.0 82-83 0.35 0.0 0.0 0.0 0.0 84-85 0.35 0.0 0.0 0.0 0.0 86-87 0.55 0.0 0.0 0.0 0.0 88-89 0.6875 0.0 0.0 0.0 0.0 90-91 0.7749999999999999 0.0 0.0 0.0 0.0 92-93 0.85 0.0 0.0 0.0 0.0 94-95 1.0 0.0 0.0 0.0 0.0 96-97 1.0875 0.0 0.0 0.0 0.0 98-99 1.325 0.0 0.0 0.0 0.0 100-101 1.6625 0.0 0.0 0.0 0.0 102-103 1.8 0.0 0.0 0.0 0.0 104-105 1.925 0.0 0.0 0.0 0.0 106-107 2.1500000000000004 0.0 0.0 0.0 0.0 108-109 2.4875 0.0 0.0 0.0 0.0 110-111 2.625 0.0 0.0 0.0 0.0 112-113 2.8625 0.0 0.0 0.0 0.0 114-115 3.2125 0.0 0.0 0.0 0.0 116-117 3.7375 0.0 0.0 0.0 0.0 118-119 4.0625 0.0 0.0 0.0 0.0 120-121 4.525 0.0 0.0 0.0 0.0 122-123 4.8375 0.0 0.0 0.0 0.0 124-125 5.125 0.0 0.0 0.0 0.0 126-127 5.45 0.0 0.0 0.0 0.0 128-129 5.85 0.0 0.0 0.0 0.0 130-131 6.362500000000001 0.0 0.0 0.0 0.0 132-133 6.9125 0.0 0.0 0.0 0.0 134-135 7.625 0.0 0.0 0.0 0.0 136-137 8.4625 0.0 0.0 0.0 0.0 138-139 8.9625 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CAGTTTT 10 0.006830828 145.0 6 GCCAGTT 10 0.006830828 145.0 4 ATTGCTT 10 0.006830828 145.0 5 GACAATT 10 0.006830828 145.0 1 TGGGCTG 10 0.006830828 145.0 145 AGTTTTG 10 0.006830828 145.0 7 ACAATTG 10 0.006830828 145.0 2 CATATTC 20 3.5877043E-4 108.75 4 CCATATT 25 8.7132835E-4 87.0 3 >>END_MODULE SRR26075391 read2 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR26075391_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.26825 37.0 37.0 37.0 37.0 37.0 2 36.301 37.0 37.0 37.0 37.0 37.0 3 36.177 37.0 37.0 37.0 37.0 37.0 4 36.3475 37.0 37.0 37.0 37.0 37.0 5 36.3255 37.0 37.0 37.0 37.0 37.0 6 36.2475 37.0 37.0 37.0 37.0 37.0 7 36.32 37.0 37.0 37.0 37.0 37.0 8 36.4015 37.0 37.0 37.0 37.0 37.0 9 36.3415 37.0 37.0 37.0 37.0 37.0 10-14 36.3221 37.0 37.0 37.0 37.0 37.0 15-19 36.2723 37.0 37.0 37.0 37.0 37.0 20-24 36.251 37.0 37.0 37.0 37.0 37.0 25-29 36.187 37.0 37.0 37.0 37.0 37.0 30-34 36.052299999999995 37.0 37.0 37.0 37.0 37.0 35-39 36.0755 37.0 37.0 37.0 37.0 37.0 40-44 36.0204 37.0 37.0 37.0 37.0 37.0 45-49 35.9486 37.0 37.0 37.0 37.0 37.0 50-54 35.8324 37.0 37.0 37.0 37.0 37.0 55-59 35.873400000000004 37.0 37.0 37.0 37.0 37.0 60-64 35.9282 37.0 37.0 37.0 37.0 37.0 65-69 35.79109999999999 37.0 37.0 37.0 37.0 37.0 70-74 35.641 37.0 37.0 37.0 37.0 37.0 75-79 35.6671 37.0 37.0 37.0 37.0 37.0 80-84 35.713499999999996 37.0 37.0 37.0 37.0 37.0 85-89 35.7162 37.0 37.0 37.0 37.0 37.0 90-94 35.5509 37.0 37.0 37.0 37.0 37.0 95-99 35.64960000000001 37.0 37.0 37.0 37.0 37.0 100-104 35.520300000000006 37.0 37.0 37.0 37.0 37.0 105-109 35.4795 37.0 37.0 37.0 37.0 37.0 110-114 35.4703 37.0 37.0 37.0 37.0 37.0 115-119 35.3571 37.0 37.0 37.0 34.6 37.0 120-124 35.28415 37.0 37.0 37.0 32.2 37.0 125-129 35.201699999999995 37.0 37.0 37.0 32.2 37.0 130-134 35.2401 37.0 37.0 37.0 34.6 37.0 135-139 35.05525 37.0 37.0 37.0 25.0 37.0 140-144 35.11255 37.0 37.0 37.0 29.8 37.0 145-149 35.034749999999995 37.0 37.0 37.0 25.0 37.0 150-151 34.660624999999996 37.0 37.0 37.0 25.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.0 1101 2 0.0 1101 3 0.0 1101 4 0.0 1101 5 0.0 1101 6 0.0 1101 7 0.0 1101 8 0.0 1101 9 0.0 1101 10-14 0.0 1101 15-19 0.0 1101 20-24 0.0 1101 25-29 0.0 1101 30-34 0.0 1101 35-39 0.0 1101 40-44 0.0 1101 45-49 0.0 1101 50-54 0.0 1101 55-59 0.0 1101 60-64 0.0 1101 65-69 0.0 1101 70-74 0.0 1101 75-79 0.0 1101 80-84 0.0 1101 85-89 0.0 1101 90-94 0.0 1101 95-99 0.0 1101 100-104 0.0 1101 105-109 0.0 1101 110-114 0.0 1101 115-119 0.0 1101 120-124 0.0 1101 125-129 0.0 1101 130-134 0.0 1101 135-139 0.0 1101 140-144 0.0 1101 145-149 0.0 1101 150-151 0.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 12 1.0 13 3.0 14 3.0 15 3.0 16 5.0 17 0.0 18 1.0 19 5.0 20 3.0 21 2.0 22 7.0 23 8.0 24 12.0 25 14.0 26 7.0 27 17.0 28 15.0 29 25.0 30 21.0 31 33.0 32 51.0 33 97.0 34 212.0 35 738.0 36 2518.0 37 199.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 42.11052763190798 22.005501375343837 12.203050762690673 23.680920230057513 2 28.199999999999996 23.175 28.199999999999996 20.424999999999997 3 23.175 25.05 32.925 18.85 4 24.575 33.175 23.825 18.425 5 25.2 34.65 22.225 17.925 6 22.7 38.775 21.875 16.650000000000002 7 20.424999999999997 22.525000000000002 38.65 18.4 8 23.474999999999998 24.95 26.325 25.25 9 22.900000000000002 24.55 29.099999999999998 23.45 10-14 23.345 29.95 25.724999999999998 20.979999999999997 15-19 23.794999999999998 27.650000000000002 27.405 21.15 20-24 24.279999999999998 29.12 26.224999999999998 20.375 25-29 23.78 28.939999999999998 26.665 20.615 30-34 23.78 28.305000000000003 26.605 21.310000000000002 35-39 24.4 28.025 26.845000000000002 20.73 40-44 24.310000000000002 28.525 26.705000000000002 20.46 45-49 23.155 28.18 27.0 21.665 50-54 23.025000000000002 29.265 27.205000000000002 20.505000000000003 55-59 23.799999999999997 27.77 27.815 20.615 60-64 24.03 27.250000000000004 27.575 21.145 65-69 24.23 28.694999999999997 26.56 20.515 70-74 24.515 28.07 26.31 21.105 75-79 24.195 28.605000000000004 26.775 20.424999999999997 80-84 23.044999999999998 28.28 27.589999999999996 21.085 85-89 23.955000000000002 28.634999999999998 26.919999999999998 20.49 90-94 23.785 28.505000000000003 27.224999999999998 20.485 95-99 24.455 28.395 27.034999999999997 20.115 100-104 23.805 28.599999999999998 26.82 20.775 105-109 24.505 27.800000000000004 26.939999999999998 20.755000000000003 110-114 24.315 28.685 26.13 20.87 115-119 24.265 28.555000000000003 26.57 20.61 120-124 24.616230811540575 28.551427571378568 26.756337816890845 20.07600380019001 125-129 26.02 28.37 25.775 19.835 130-134 25.2 27.245 27.32 20.235 135-139 25.473821073160973 28.484272640896137 26.048907336100413 19.992998949842477 140-144 25.618842826423965 28.40926138920838 26.253938090713607 19.717957693654046 145-149 26.34158539634909 27.866966741685424 26.076519129782444 19.714928732183047 150-151 24.50306288286036 28.46605825728216 26.490811351418923 20.540067508438558 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.5 6 0.5 7 1.0 8 1.5 9 1.0 10 0.5 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.5 18 1.5 19 1.5 20 0.5 21 0.5 22 1.5 23 3.0 24 2.5 25 1.5 26 3.5 27 7.0 28 7.0 29 6.0 30 12.5 31 16.0 32 16.0 33 21.5 34 33.0 35 54.0 36 72.0 37 89.5 38 115.0 39 146.5 40 188.5 41 217.0 42 255.0 43 279.5 44 283.0 45 301.5 46 287.5 47 254.0 48 237.0 49 208.0 50 160.5 51 136.0 52 121.0 53 110.0 54 88.0 55 58.5 56 40.0 57 28.5 58 28.0 59 17.5 60 14.0 61 16.0 62 9.0 63 5.5 64 5.5 65 6.0 66 4.0 67 1.0 68 1.5 69 1.0 70 0.5 71 1.0 72 1.0 73 0.5 74 0.0 75 0.5 76 1.0 77 0.5 78 1.5 79 1.5 80 0.0 81 0.0 82 0.0 83 0.5 84 1.0 85 1.0 86 1.0 87 1.0 88 1.5 89 1.0 90 0.5 91 0.5 92 0.5 93 0.5 94 1.0 95 1.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.025 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.005 125-129 0.0 130-134 0.0 135-139 0.015 140-144 0.015 145-149 0.025 150-151 0.0125 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 60.0 #Duplication Level Percentage of deduplicated Percentage of total 1 64.04166666666666 38.425 2 20.333333333333332 24.4 3 8.041666666666666 14.475 4 4.125 9.9 5 1.5 4.5 6 0.9583333333333333 3.45 7 0.4166666666666667 1.7500000000000002 8 0.25 1.2 9 0.20833333333333334 1.125 >10 0.125 0.775 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CGCTGCGTGATCTTGTTTCATACAATTTCAAGCACAATGATGCTAATGGA 11 0.27499999999999997 No Hit GTTTGCTGCGGTTTCGCCTTGACCGCGGGAAGGAGACATAACGATAGCGA 10 0.25 No Hit GGGTCAGGGAATTTCGGGGTCGCCAGGTTGATGAGGGACAAACAGGCTGA 10 0.25 No Hit TTCTGATGGAAATGTTGTTGTGCTCAATGGTCAAAGTGGTGGTGCTCCCG 9 0.22499999999999998 No Hit AAGCTGAGGACATTGTCAAAGAACAAGTTAGACTCCTTTACAAGAGACAC 9 0.22499999999999998 No Hit TCTCTCTTAGCCTCATTGTTTCAAGAAAATGGGTAGCCTTGAAACAGAGA 9 0.22499999999999998 No Hit CTTCAGCTAGTCGATATGGAGTGTAGCTACCTGACAGTGGACTTTTTTCG 9 0.22499999999999998 No Hit AGAGCAATTACCATGTCTACGGGTCTCACTGTCAAGGCTTCCTTTACCAA 9 0.22499999999999998 No Hit GCTTGTTCTTCATGCAGCAAAAAACTCCAACATAAGTGTCCTTCTTGCTC 8 0.2 No Hit AGGAAACTTGCGTTACATATCATCAGGACAAATAGGTTTCCTGGATTTTG 8 0.2 No Hit ATTGATTCAGCTGAGATTGGGAAGAATAAGCAGCCTCATGTGTCCGTCTG 8 0.2 No Hit AGGGAATATGGTCAGGCATGTTAGGAGGGACCGTTTTGCAAACTATAATC 8 0.2 No Hit TGACAGCCAAGGAAAGTGCTACTTGGCTTGCAATTATCAACCAAGAGGAA 8 0.2 No Hit GGGATTGATGTGCGTCTTTGTGATGTTGGTGCTGCCATCCAAGAAGTCAT 8 0.2 No Hit AGGTGGTCAGCAATTGCATCAAGATTACCAGGAAGGACAGACAATGAGAT 7 0.17500000000000002 No Hit GCAACTTCTCTAAGCGGTATTTAAAGTACTTGACCAAAAAGTACTTGAAG 7 0.17500000000000002 No Hit TCTAGTTTCCCGAAGCTTTGTAGAGTTTGGTTGGAGTACTTCCTACCCGC 7 0.17500000000000002 No Hit TGGCACCCTCAAAGCTCATCTTCACCCTCTTCATCCTCCTTTCCTCCCTC 7 0.17500000000000002 No Hit GGACTCGGGGGGAGCATTTTATAGTGAGATGGGCAATCCCCCAGCTCCAT 7 0.17500000000000002 No Hit AGGACGAGGAGGATGGAGAAGAAGGAGAGAAGCAAGAAGAAGAAGACGAA 7 0.17500000000000002 No Hit GACCGATCGATCCGATCAGAGTTTGATGGGAGAGATTAATGAAGAAGACG 7 0.17500000000000002 No Hit CCTGTCTGAGTTCTTGACCTCAGACTTTCTTTAGTTGCTGTCTTTCATCT 7 0.17500000000000002 No Hit GTGGCATCATCACCACCATTTTACTGTGGATCACCACCATGTAGGGTATC 7 0.17500000000000002 No Hit GTCTCGAATCAGGTAGCGAAAGAAGAACAAGGGAGGCCTCGTGCCTTTCT 7 0.17500000000000002 No Hit GGAAGCAGAAGAAAACTTCAAAGGCAGTTTGGAGAATGATTTAGCTTGCC 6 0.15 No Hit CCCAAAAGACCACTACTCTTCCTCTCTATAATTTTCTAGGGTTTAGCAAT 6 0.15 No Hit ACCACTCTTCCTCTCTCTTTCAAACCCTAACCCCCCACACCGGTTTCTCC 6 0.15 No Hit AATTGGTTTAATGCCCATGATTGATCAGGGTGAAAAGGATGACAAAATCA 6 0.15 No Hit ATGGGATCCCATAAAACAATGCTTGGTTCTGGAGCATATGATTACATATT 6 0.15 No Hit GTTTTACTAGGCTAGACTAGCGTACGAGCACTATGGTCAGTAATTCCTGG 6 0.15 No Hit GTTGTTGGCTTCTGCTTTCTGCAACTGAACTTCATTAGCTTCGATATCTT 6 0.15 No Hit ATGATGAAGAGGTAGTTCTTGGAGATGAGATACGGCTTCTTGATAGGAAT 6 0.15 No Hit TATTGGTGGAAATAAGGATTTGATTAAATTGCTTGAGGTTGCAAGAGCTT 6 0.15 No Hit GTTCGATTTCTTAAAGGACTGAATATTCGGTGGCAGTATGGGATTTCTAA 6 0.15 No Hit GTTCGGACTAATTGGAATGAAGTTCTTGTCATGAAGGAAATCAGCAGAGA 6 0.15 No Hit GTGTAGTTTTTATGATGTTGTTCGTGTTGAGGAAAGTTTCAATGAGGTGC 6 0.15 No Hit TGGTCGTAGAGAAGCCTACACACCTATACTTAGTAATTGAAAAGGTAACT 6 0.15 No Hit TATTACCCTCAAGGATTACGAGATTCATGATGGCATGGGCCTCGAGCTCT 6 0.15 No Hit TTTCTGCCCAGTGCTCTGAATGTCAAAGTGAAGAAATTCAACCAAGCGCG 6 0.15 No Hit CCCAATTTCCCTCTCCTGGTCATCAGCACAGGCAGCCACCTCTCCCTTTG 6 0.15 No Hit GTAAGATTGATCACTTATTATCATGTTGATGTCTTATAGGCTATAGTAAG 6 0.15 No Hit ATCACGAGTAATGTTTTGAGTGACGGGCCATTTGTGATTTGGCAGATGAA 6 0.15 No Hit GTGAAATTTGATCTCAATTTATCTACCAATTTGAGTCAAGTGATCGATCC 6 0.15 No Hit AGTTCGTTTTCGGTGCAAGAAGTGAATGAGAGTGGAGGGAAGTGGGAGGT 6 0.15 No Hit GACAAGAGAGCCAAAGGAAGAAAATGTCACTCTTGGCCCAACTGTCCGGG 6 0.15 No Hit GTGGATCAAGGTATTCTTCCAGGGGCGGATCTGATGGTGGTAGCTGGAGG 6 0.15 No Hit GCAGCAGCCACATTAAAATTCTCAGCACTAATTGGTGGACCCAGTTGATG 6 0.15 No Hit GTGGGTTCTTGTTACTGGTCCATTTCTTGGATGTCTATCTGTTCATTATG 5 0.125 No Hit CCTTTGCACTCATAGCTGAAGAGTTGAAATTTGTAGCCAGTCAGTTCAGG 5 0.125 No Hit GGCATATCAAACATGCCATGGAAATAAATCCTTTTAGCTTTTTGACTAAT 5 0.125 No Hit TTGGGTGAAGGAGCTACAAAAACATGGTAGCCCTGATATAGTCATGGCTT 5 0.125 No Hit CCCCATATTTCAGATCACAAAAGATAGCGAGAGGAGGCAAATCTTGATAT 5 0.125 No Hit GAGGACTTCCTGATGATCAAGAAACCAAGTTATATATAGCTACAGATGGT 5 0.125 No Hit GGAAGAATTAATCCTTACATGTCCAGCCCTTGCCACATTGAGTTGACTTT 5 0.125 No Hit TGGGAAACAACATAACTGAAGTGACCAGCATGCCAGTGAACAGTTCTGCT 5 0.125 No Hit AATTATTGAGGCCTATCTACAGTAATATAAATTTGATTTCTATGGTCATA 5 0.125 No Hit GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGT 5 0.125 No Hit AAGATGATAAAACCTGTTCACCAGCTGTAGCCGAGAAACAAGATTTGGAA 5 0.125 No Hit GAAACTTCTGAAGGACATCCAAGAGTCTCCGATTCCGATTAATATTGTTC 5 0.125 No Hit GGAAAGAAAAATAAGAAAGGAAGACGAAGAATCCTCTCGGAGACGGGATT 5 0.125 No Hit TTGAGATTCAAACTCAAAGATTAGAGCTTTTGACTGCTCAAAGTATGGCA 5 0.125 No Hit GGGAAGCACGAATCCTTTATTGGAGACTATATCAAGTTTCATTGAACTGA 5 0.125 No Hit GTTAGATAAGGAATTTATACCTGATCTTGCCACATATAATAGGTTCATAG 5 0.125 No Hit ATTGCAGCCGTTCATAACCCTAAAGAAGCGATACCTCTCTTCGAAAACCA 5 0.125 No Hit TGCTGATGGAAGCCCTGTTACAGTTTCCTGGAGTGGTGATTCAAGACGGA 5 0.125 No Hit CTCTTTCAATCAATCAGACGACTATGGACTCATCTTCCCGTCGTCTCAAA 5 0.125 No Hit GAAATAGATGCATCATCGAGCTGTGTACACTTATTCAAAGTTTGCAGCAA 5 0.125 No Hit GCTAAACGACATATCTTCGACCAGAAACTGAATAGGAAGCTTATGCATAT 5 0.125 No Hit CCAGAGCCATGCTGTCCTTGCACTCCAGGAGGCTGCTGAGGCTTATCTCG 5 0.125 No Hit CTAACTGGCAAGATGACCAAAATAAGGGATCAAAGGACAGAGCACCAGGT 5 0.125 No Hit GAAGAGGAAGGCCCTGCTGGCACTTAAGACAGAGGAAAGAAAGGTGGATA 5 0.125 No Hit TTTTGATCGCTTACTGGACCAATTATCACAGATTGAGATAAACAGCTTAG 5 0.125 No Hit GCTTTTCGGTTAACCGGTATTGGACCACCGGCTCATGATAACACGGACCA 5 0.125 No Hit GCTTCCCTAACAGAACCTTAGTTCCGCTTGCCACAAATCCACAAACTGAC 5 0.125 No Hit CGTCAATTTGATTCTAGGATGAATGAGCTGCTCACAGCTGAAGGACAGGA 5 0.125 No Hit GGTATCAACTATGAATGCAGAGGTTGCAAGGGAATACCTGGAGAAAGTGG 5 0.125 No Hit GCCAAGGACACAGGCTTCCCTTGGCTACCAGCCTGCTGCAGGCCCAGTTA 5 0.125 No Hit GGGGGGGCAAACAATGGCTGCACGCTACTGGTGCCATATGTGCTCTCAAA 5 0.125 No Hit GTTGGGGTGGAGAAGGGCTGAGGAAGACTCTGTTTCGCTGGTCATCTTCA 5 0.125 No Hit ATGGGAACTCTTAATATCAGGGGTAAAACCCAACAAAGTATGTGCGCAAC 5 0.125 No Hit GGAGTTATCAAGAGCGTGGAGAAGAAGGATCCCTCTGGTGCTAAGGTGAC 5 0.125 No Hit GGACTGACCACTGAAGTTAAATCTGTTGAGATGCACCATGAAGCTCTTCA 5 0.125 No Hit GTTTGATTCCTGGTTTCTCATCAAGGTTTGCTGCTGCTTCTTCTGAAGAT 5 0.125 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0 0.0 0.0 0.0 0.0 64-65 0.0 0.0 0.0 0.0 0.0 66-67 0.0 0.0 0.0 0.0 0.0 68-69 0.0 0.0 0.0 0.0 0.0 70-71 0.0 0.0 0.0 0.0 0.0 72-73 0.075 0.0 0.0 0.0 0.0 74-75 0.15 0.0 0.0 0.0 0.0 76-77 0.25 0.0 0.0 0.0 0.0 78-79 0.275 0.0 0.0 0.0 0.0 80-81 0.3125 0.0 0.0 0.0 0.0 82-83 0.325 0.0 0.0 0.0 0.0 84-85 0.325 0.0 0.0 0.0 0.0 86-87 0.525 0.0 0.0 0.0 0.0 88-89 0.6875 0.0 0.0 0.0 0.0 90-91 0.7749999999999999 0.0 0.0 0.0 0.0 92-93 0.85 0.0 0.0 0.0 0.0 94-95 1.0 0.0 0.0 0.0 0.0 96-97 1.1 0.0 0.0 0.0 0.0 98-99 1.35 0.0 0.0 0.0 0.0 100-101 1.675 0.0 0.0 0.0 0.0 102-103 1.8125 0.0 0.0 0.0 0.0 104-105 1.95 0.0 0.0 0.0 0.0 106-107 2.1875 0.0 0.0 0.0 0.0 108-109 2.5375 0.0 0.0 0.0 0.0 110-111 2.675 0.0 0.0 0.0 0.0 112-113 2.9375 0.0 0.0 0.0 0.0 114-115 3.2875 0.0 0.0 0.0 0.0 116-117 3.8125 0.0 0.0 0.0 0.0 118-119 4.137499999999999 0.0 0.0 0.0 0.0 120-121 4.575 0.0 0.0 0.0 0.0 122-123 4.887499999999999 0.0 0.0 0.0 0.0 124-125 5.175000000000001 0.0 0.0 0.0 0.0 126-127 5.5125 0.0 0.0 0.0 0.0 128-129 5.95 0.0 0.0 0.0 0.0 130-131 6.4625 0.0 0.0 0.0 0.0 132-133 7.012499999999999 0.0 0.0 0.0 0.0 134-135 7.7125 0.0 0.0 0.0 0.0 136-137 8.525 0.0 0.0 0.0 0.0 138-139 9.0125 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CAGCTAG 10 0.006830828 145.0 4 GCTAGTC 10 0.006830828 145.0 6 AGCTAGT 10 0.006830828 145.0 5 TAGTCGA 10 0.006830828 145.0 8 TCAGCTA 10 0.006830828 145.0 3 TTCTTTG 10 0.006830828 145.0 6 AGTCGAT 10 0.006830828 145.0 9 GAATTTC 10 0.006830828 145.0 2 CTAGTCG 10 0.006830828 145.0 7 ATATCAA 10 0.006830828 145.0 4 >>END_MODULE Read 614758 spots for SRR26075391.sra Written 614758 spots for SRR26075391.sra Read 614758 spots for SRR26075391.sra Written 614758 spots for SRR26075391.sra Read 614758 spots for SRR26075391.sra Written 614758 spots for SRR26075391.sra Read 614758 spots for SRR26075391.sra Written 614758 spots for SRR26075391.sra Read 614758 spots for SRR26075391.sra Written 614758 spots for SRR26075391.sra Read 614758 spots for SRR26075391.sra Written 614758 spots for SRR26075391.sra Read 614758 spots for SRR26075391.sra Written 614758 spots for SRR26075391.sra Read 614758 spots for SRR26075391.sra Written 614758 spots for SRR26075391.sra Read 614758 spots for SRR26075391.sra Written 614758 spots for SRR26075391.sra Read 614758 spots for SRR26075391.sra Written 614758 spots for SRR26075391.sra Read 614758 spots for SRR26075391.sra Written 614758 spots for SRR26075391.sra Read 614758 spots for SRR26075391.sra Written 614758 spots for SRR26075391.sra Read 614758 spots for SRR26075391.sra Written 614758 spots for SRR26075391.sra Read 614776 spots for SRR26075391.sra Written 614776 spots for SRR26075391.sra Read 614758 spots for SRR26075391.sra Written 614758 spots for SRR26075391.sra Read 614758 spots for SRR26075391.sra Written 614758 spots for SRR26075391.sra Read 614758 spots for SRR26075391.sra Written 614758 spots for SRR26075391.sra Read 614758 spots for SRR26075391.sra Written 614758 spots for SRR26075391.sra Read 614758 spots for SRR26075391.sra Written 614758 spots for SRR26075391.sra Read 614758 spots for SRR26075391.sra Written 614758 spots for SRR26075391.sra SRR ids: ['SRR26075391.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_wy639tqv SRR26075391.sra spots: 12295178 blocks: [[1, 614758], [614759, 1229516], [1229517, 1844274], [1844275, 2459032], [2459033, 3073790], [3073791, 3688548], [3688549, 4303306], [4303307, 4918064], [4918065, 5532822], [5532823, 6147580], [6147581, 6762338], [6762339, 7377096], [7377097, 7991854], [7991855, 8606612], [8606613, 9221370], [9221371, 9836128], [9836129, 10450886], [10450887, 11065644], [11065645, 11680402], [11680403, 12295178]] SRR26075391 file size 4533384 SRR26075391 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o SRR26075391 SRR26075391_1.fastq SRR26075391_2.fastq Input file: SRR26075391_1.fastq Paired file: SRR26075391_2.fastq trimmed: SRR26075391-trimmed-pair1.fastq, SRR26075391-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Wed Feb 12 00:20:51 2025 >> started Wed Feb 12 00:21:06 2025 >> done (14.260s) 12295178 read pairs processed; of these: 64 ( 0.00%) short read pairs filtered out after trimming by size control 9837 ( 0.08%) empty read pairs filtered out after trimming by size control 12285277 (99.92%) read pairs available; of these: 1801897 (14.67%) trimmed read pairs available after processing 10483380 (85.33%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 18 8 0.00% 19 5 0.00% 20 4 0.00% 21 8 0.00% 22 9 0.00% 23 4 0.00% 24 3 0.00% 25 4 0.00% 26 11 0.00% 27 7 0.00% 28 9 0.00% 29 11 0.00% 30 13 0.00% 31 10 0.00% 32 16 0.00% 33 11 0.00% 34 16 0.00% 35 21 0.00% 36 11 0.00% 37 7 0.00% 38 15 0.00% 39 20 0.00% 40 17 0.00% 41 19 0.00% 42 21 0.00% 43 27 0.00% 44 33 0.00% 45 30 0.00% 46 35 0.00% 47 55 0.00% 48 27 0.00% 49 63 0.00% 50 79 0.00% 51 72 0.00% 52 68 0.00% 53 71 0.00% 54 99 0.00% 55 109 0.00% 56 121 0.00% 57 117 0.00% 58 202 0.00% 59 197 0.00% 60 219 0.00% 61 248 0.00% 62 264 0.00% 63 426 0.00% 64 418 0.00% 65 460 0.00% 66 442 0.00% 67 540 0.00% 68 628 0.01% 69 750 0.01% 70 794 0.01% 71 991 0.01% 72 1140 0.01% 73 1364 0.01% 74 1525 0.01% 75 1825 0.01% 76 1800 0.01% 77 1964 0.02% 78 2327 0.02% 79 2522 0.02% 80 2839 0.02% 81 3172 0.03% 82 3666 0.03% 83 4071 0.03% 84 4431 0.04% 85 5362 0.04% 86 5314 0.04% 87 5833 0.05% 88 6419 0.05% 89 6557 0.05% 90 7053 0.06% 91 7698 0.06% 92 8355 0.07% 93 9259 0.08% 94 10053 0.08% 95 10866 0.09% 96 11276 0.09% 97 11793 0.10% 98 12269 0.10% 99 12839 0.10% 100 13127 0.11% 101 13595 0.11% 102 14716 0.12% 103 15989 0.13% 104 17151 0.14% 105 17236 0.14% 106 17926 0.15% 107 19528 0.16% 108 19313 0.16% 109 20266 0.16% 110 20179 0.16% 111 20793 0.17% 112 21707 0.18% 113 22799 0.19% 114 23451 0.19% 115 25309 0.21% 116 25519 0.21% 117 26580 0.22% 118 27231 0.22% 119 27404 0.22% 120 27702 0.23% 121 28685 0.23% 122 28936 0.24% 123 30330 0.25% 124 31397 0.26% 125 31851 0.26% 126 33601 0.27% 127 34133 0.28% 128 34665 0.28% 129 35345 0.29% 130 35478 0.29% 131 36406 0.30% 132 36743 0.30% 133 37757 0.31% 134 38648 0.31% 135 39031 0.32% 136 39452 0.32% 137 40707 0.33% 138 42828 0.35% 139 42765 0.35% 140 42875 0.35% 141 43494 0.35% 142 44858 0.37% 143 45163 0.37% 144 45637 0.37% 145 47211 0.38% 146 47087 0.38% 147 49044 0.40% 148 49098 0.40% 149 49535 0.40% 150 50159 0.41% 151 10483380 85.33% 12285277 reads passed initial QC criterion=sequence-density sequence-density=0.21 sequence-density-rank=1 fanout-score=3.98 fanout-score-rank=28 prefix-density=0.35 prefix-fanout=2.3 sequence=CACTTGCAGCCATTCTCAGCACC criterion=fanout-score sequence-density=0.03 sequence-density-rank=34 fanout-score=118.74 fanout-score-rank=1 prefix-density=0.25 prefix-fanout=14.3 sequence=TTCTTGATAAAATCACGATGACCAGGGGCATCAATGACAGTGCAGTAGTACCTAGTTGTCTCAAACTTCCAGAGAGCAATGTCAATTGTGATACCACGCTCACGCTCAGCCTTAAGCTTGTCCAAAACCCAGGCATACTTGAATGACCTTTTGTTCATCTCAGCAGCTTCCTTCTC criterion=sequence-density sequence-density=0.27 sequence-density-rank=1 fanout-score=2.81 fanout-score-rank=29 prefix-density=0.28 prefix-fanout=2.8 sequence=ATGTACCCTGACTT criterion=fanout-score sequence-density=0.01 sequence-density-rank=36 fanout-score=193.15 fanout-score-rank=1 prefix-density=0.12 prefix-fanout=12.5 sequence=AGAAAAGATAAGCTAGGCAAGATGGTTTTACTA SRR26075391 testing PE reads STAR mapping to Ensembl genome Started job on | Feb 12 00:21:47 Started mapping on | Feb 12 00:21:47 Finished on | Feb 12 00:24:18 Mapping speed, Million of reads per hour | 292.89 Number of input reads | 12285277 Average input read length | 294 UNIQUE READS: Uniquely mapped reads number | 10938915 Uniquely mapped reads % | 89.04% Average mapped length | 293.17 Number of splices: Total | 9358959 Number of splices: Annotated (sjdb) | 9126829 Number of splices: GT/AG | 9184593 Number of splices: GC/AG | 133507 Number of splices: AT/AC | 10350 Number of splices: Non-canonical | 30509 Mismatch rate per base, % | 0.41% Deletion rate per base | 0.03% Deletion average length | 2.97 Insertion rate per base | 0.02% Insertion average length | 2.58 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 297823 % of reads mapped to multiple loci | 2.42% Number of reads mapped to too many loci | 43819 % of reads mapped to too many loci | 0.36% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 7.98% % of reads unmapped: other | 0.20% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 1048539 1048539 1048539 N_multimapping 297823 297823 297823 N_noFeature 362327 10817413 428101 N_ambiguous 126697 886 70346 UnstrandedReadsAssigned:10449891 PositiveStrandReadsAssigned:120616 NegativeStrandReadsAssigned:10440468 Dataset is classified negative stranded MeadianReadLen=151 20thPercentileLength=151 echo kmer=147 SRR26075391 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,400 [index] number of k-mers: 62,057,036 [index] number of equivalence classes: 130,681 [quant] running in paired-end mode [quant] will process pair 1: SRR26075391-trimmed-pair1.fastq SRR26075391-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 12,285,277 reads, 10,551,886 reads pseudoaligned [quant] estimated average fragment length: 215.859 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 997 rounds 52401 SRR26075391.ke.tsv 34699 SRR26075391.se.tsv 87100 total ==> SRR26075391.ke.tsv <== target_id length eff_length est_counts tpm Potri.005G200100.1.v4.1 2018 1803.14 1664 84.4155 Potri.005G024800.1.v4.1 1035 820.141 547 61.0095 Potri.004G059700.1.v4.1 961 746.148 2 0.24519 Potri.007G009000.2.v4.1 1416 1201.14 0 0 Potri.003G141000.2.v4.1 2943 2728.14 419 14.049 Potri.016G087400.1.v4.1 270 88.446 530 548.146 Potri.015G069301.1.v4.1 564 350.947 0 0 Potri.010G195200.1.v4.1 1773 1558.14 171 10.0389 Potri.012G127500.1.v4.1 977 762.148 5614 673.801 ==> SRR26075391.se.tsv <== Potri.001G166300.v4.1 0 Potri.001G448400.v4.1 104 Potri.001G233950.v4.1 0 Potri.001G122700.v4.1 144 Potri.001G212900.v4.1 0 Potri.001G182400.v4.1 0 Potri.001G256600.v4.1 0 Potri.001G040500.v4.1 7 Potri.001G416900.v4.1 1 Potri.001G452600.v4.1 227 SRR26075391 completed mapping pipeline successfully