Starting /dee2/code/volunteer_pipeline.sh SRR26075392 current disk space = 3048999755776 free memory = 1534784152 SRR26075392 SRAfilesize bedbc3b998da3b6b88ca33508d75d182 SRR26075392.sra SRR26075392.sra file validated SRR26075392 is paired end SRR26075392 is conventional basespace SRR26075392 read1 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR26075392_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.5685 37.0 37.0 37.0 37.0 37.0 2 36.555 37.0 37.0 37.0 37.0 37.0 3 36.669 37.0 37.0 37.0 37.0 37.0 4 36.6435 37.0 37.0 37.0 37.0 37.0 5 36.704 37.0 37.0 37.0 37.0 37.0 6 36.61 37.0 37.0 37.0 37.0 37.0 7 36.659 37.0 37.0 37.0 37.0 37.0 8 36.6655 37.0 37.0 37.0 37.0 37.0 9 36.669 37.0 37.0 37.0 37.0 37.0 10-14 36.650349999999996 37.0 37.0 37.0 37.0 37.0 15-19 36.638799999999996 37.0 37.0 37.0 37.0 37.0 20-24 36.6144 37.0 37.0 37.0 37.0 37.0 25-29 36.5413 37.0 37.0 37.0 37.0 37.0 30-34 36.4559 37.0 37.0 37.0 37.0 37.0 35-39 36.432900000000004 37.0 37.0 37.0 37.0 37.0 40-44 36.407799999999995 37.0 37.0 37.0 37.0 37.0 45-49 36.3902 37.0 37.0 37.0 37.0 37.0 50-54 36.3291 37.0 37.0 37.0 37.0 37.0 55-59 36.304899999999996 37.0 37.0 37.0 37.0 37.0 60-64 36.281 37.0 37.0 37.0 37.0 37.0 65-69 36.1539 37.0 37.0 37.0 37.0 37.0 70-74 36.109300000000005 37.0 37.0 37.0 37.0 37.0 75-79 36.0648 37.0 37.0 37.0 37.0 37.0 80-84 35.933899999999994 37.0 37.0 37.0 37.0 37.0 85-89 35.9164 37.0 37.0 37.0 37.0 37.0 90-94 35.8837 37.0 37.0 37.0 37.0 37.0 95-99 35.843199999999996 37.0 37.0 37.0 37.0 37.0 100-104 35.7305 37.0 37.0 37.0 37.0 37.0 105-109 35.7177 37.0 37.0 37.0 37.0 37.0 110-114 35.704299999999996 37.0 37.0 37.0 37.0 37.0 115-119 35.6298 37.0 37.0 37.0 37.0 37.0 120-124 35.592499999999994 37.0 37.0 37.0 37.0 37.0 125-129 35.5595 37.0 37.0 37.0 37.0 37.0 130-134 35.3551 37.0 37.0 37.0 34.6 37.0 135-139 35.162400000000005 37.0 37.0 37.0 29.8 37.0 140-144 35.096799999999995 37.0 37.0 37.0 25.0 37.0 145-149 35.056000000000004 37.0 37.0 37.0 27.4 37.0 150-151 34.961 37.0 37.0 37.0 25.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.0 1101 2 0.0 1101 3 0.0 1101 4 0.0 1101 5 0.0 1101 6 0.0 1101 7 0.0 1101 8 0.0 1101 9 0.0 1101 10-14 0.0 1101 15-19 0.0 1101 20-24 0.0 1101 25-29 0.0 1101 30-34 0.0 1101 35-39 0.0 1101 40-44 0.0 1101 45-49 0.0 1101 50-54 0.0 1101 55-59 0.0 1101 60-64 0.0 1101 65-69 0.0 1101 70-74 0.0 1101 75-79 0.0 1101 80-84 0.0 1101 85-89 0.0 1101 90-94 0.0 1101 95-99 0.0 1101 100-104 0.0 1101 105-109 0.0 1101 110-114 0.0 1101 115-119 0.0 1101 120-124 0.0 1101 125-129 0.0 1101 130-134 0.0 1101 135-139 0.0 1101 140-144 0.0 1101 145-149 0.0 1101 150-151 0.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 21 3.0 22 2.0 23 1.0 24 5.0 25 3.0 26 11.0 27 16.0 28 17.0 29 35.0 30 39.0 31 42.0 32 54.0 33 85.0 34 162.0 35 426.0 36 2896.0 37 203.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.092092092092095 14.93993993993994 7.207207207207207 35.76076076076076 2 22.3 12.0 33.525 32.175 3 18.05 19.775000000000002 28.749999999999996 33.425 4 22.15 24.45 26.424999999999997 26.974999999999998 5 23.05 30.599999999999998 24.375 21.975 6 21.4 35.35 21.349999999999998 21.9 7 15.7 27.675 39.1 17.525 8 19.0 24.3 30.8 25.900000000000002 9 18.025 25.025 32.95 24.0 10-14 19.592938940841126 30.17952692903936 27.444116617492625 22.783417512626894 15-19 20.330000000000002 28.415000000000003 27.894999999999996 23.36 20-24 20.145 27.62 28.715000000000003 23.52 25-29 20.064999999999998 27.450000000000003 28.685 23.799999999999997 30-34 20.54 27.66 28.625 23.175 35-39 20.32 28.15 27.465 24.065 40-44 20.215 28.005000000000003 27.675 24.104999999999997 45-49 20.26 28.134999999999998 27.66 23.945 50-54 19.97 27.63 27.425 24.975 55-59 20.919999999999998 28.535 27.365000000000002 23.18 60-64 21.029999999999998 27.61 28.189999999999998 23.169999999999998 65-69 20.48 27.98 27.515 24.025 70-74 20.46 27.505000000000003 27.975 24.060000000000002 75-79 21.029999999999998 27.944999999999997 27.205000000000002 23.82 80-84 19.650000000000002 27.38 28.294999999999998 24.675 85-89 20.13 27.36 27.785 24.725 90-94 20.825 28.555000000000003 27.279999999999998 23.34 95-99 20.830000000000002 27.605 26.85 24.715 100-104 21.125 27.55 27.915 23.41 105-109 21.08 27.525 26.740000000000002 24.654999999999998 110-114 21.41 27.665 27.089999999999996 23.835 115-119 21.055 28.005000000000003 27.200000000000003 23.74 120-124 21.735 27.544999999999998 27.215 23.505000000000003 125-129 21.205 28.305000000000003 26.745 23.745 130-134 21.97 27.805000000000003 26.58 23.645 135-139 21.165 26.889999999999997 27.61 24.335 140-144 21.455 26.695 27.365000000000002 24.485 145-149 22.1 27.634999999999998 26.71 23.555 150-151 21.9 29.7125 25.087500000000002 23.3 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1.0 1 0.5 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.5 14 0.5 15 0.0 16 0.0 17 0.0 18 0.5 19 0.5 20 0.0 21 0.5 22 2.5 23 2.5 24 1.0 25 1.5 26 3.0 27 3.5 28 8.5 29 17.0 30 20.0 31 19.5 32 15.0 33 25.0 34 49.5 35 61.0 36 71.5 37 103.5 38 128.0 39 144.0 40 170.0 41 214.5 42 250.5 43 271.0 44 272.0 45 256.0 46 270.0 47 263.5 48 238.0 49 221.0 50 192.5 51 152.5 52 111.0 53 83.0 54 60.0 55 57.5 56 59.5 57 43.5 58 27.0 59 22.0 60 27.5 61 18.0 62 7.5 63 7.5 64 7.5 65 5.5 66 3.5 67 3.5 68 2.0 69 0.5 70 0.0 71 0.5 72 0.5 73 0.5 74 0.5 75 0.0 76 0.0 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.1 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.015 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150-151 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 58.95 #Duplication Level Percentage of deduplicated Percentage of total 1 62.25614927905004 36.7 2 20.610687022900763 24.3 3 9.372349448685327 16.575 4 4.156064461407973 9.8 5 1.7811704834605597 5.25 6 0.8905852417302799 3.15 7 0.6361323155216284 2.625 8 0.16963528413910092 0.8 9 0.0 0.0 >10 0.1272264631043257 0.8 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CGTGTTGGAAATTTTCTGTCTCTTGTTCCTTTGGCTGGTATTGTGCAACG 11 0.27499999999999997 No Hit CTTCTCTTCAAAAACCAGGCTGATTTCTCATTTTCTCCATCAAGAAAGCC 11 0.27499999999999997 No Hit TTCCCGTCCTTTTATCCCTTTATTCTTCAGAACCACCTTCTGACCAGGTT 10 0.25 No Hit CGTTCGTCCAGCACCCATCCCAACAAAACAAAACCCTGAACATCCTGCAC 8 0.2 No Hit CAATAATGGAGTATGCCATCCGACTCAGAATCACTATCGCTGCATTGCAT 8 0.2 No Hit GCCAGAAAGAACACCAATATATAATTATAAAAAGTTACCTTAAATACATC 8 0.2 No Hit CCCTTCCCAAGAACTTTGAAGTACCCGAACTGAGTGACATCGATCATCGG 8 0.2 No Hit GAACAAACTTCATGCAGGTACTCTAATGCCCTAGCGGTCCCCAGAGCTAT 7 0.17500000000000002 No Hit GACTGAGCTAGTCCTGGTGGCGCCTGGCCCTGATCCGAACCAAAATCAGG 7 0.17500000000000002 No Hit CCTCTGCGTTCTCTGCTTCCACTATCTCTCCTCTCCCTGCCATTCTCTCC 7 0.17500000000000002 No Hit GTGTCTTCATCAGCATCTTGATAAATTTCTCGGAAAAATTTGTTCAAAGC 7 0.17500000000000002 No Hit GGCGGTGGCGGTGAAGTTGGCGGAGGCGGAGGCGGAGGCGGCGGCGGATG 7 0.17500000000000002 No Hit ATTGCGTTGCTGTATACGAATGTGCACATAATCTTTTGTCCCAGCACCAG 7 0.17500000000000002 No Hit TTGTAAAGATTCAACCTTTCCTTCTTTCTTCAATTCGATATATCTATCTA 7 0.17500000000000002 No Hit GTCTAAATCCTGGTAGATCTCGCTTTGAAATGTCTCTTTCGAGATGGGAA 7 0.17500000000000002 No Hit GGATGGGTATGCTGCAGATATGTGATCAGAACAAGGAATGCATTCACCAC 7 0.17500000000000002 No Hit GCCACTTTTCCAGTCATTGCCTCGTTTGGCAGCCCATTATTACTGTCAGA 7 0.17500000000000002 No Hit CGGCGTAATAATAGATGGGTAAGGGTCCCCACTGAATAGATCCGAACATA 7 0.17500000000000002 No Hit CCTCACACGAACATATGCCTGAGAAAATGCATTTCGAAACTCAGAGTCAT 7 0.17500000000000002 No Hit GGTTAGTGAAAAAAATAGCAAGATTTGGGGATATTCAACATGATGAAAGC 7 0.17500000000000002 No Hit TTTGACAGGGGCTGGAACCTTCTCCCCTAGCTCCTTAAGCTGCTTCTCAG 7 0.17500000000000002 No Hit GGGTTTTTCCTGCGTGATATTCTGCAAACTGGTCTCGGCGCGTCCCGTCA 7 0.17500000000000002 No Hit CTTGCAAGTCTTTCAAACATGACACTGTCAGCCCCTTTACAAAGTAGAAG 6 0.15 No Hit GTCAGCTCGCTTTGGTTTCCAATCAAGCATCATCCACTGCTTCTCTGCTG 6 0.15 No Hit CGACCCTCTGCATCTTTAATGGCTATATATGGCTTGGACTTTACACGATT 6 0.15 No Hit GGTAATAGTTCTTCTGAAGTTAAGTTCTTCTGTAACCTCTTATACATTGC 6 0.15 No Hit ATAGGCCTGCATGAGAGAGGGTCTAACAGTTGCTTGCAGTTCAGTCCAGG 6 0.15 No Hit ATAACAGAGCAAGCATTAGGGTTTTCATCACTGAAAATCTGAGGAGCATA 6 0.15 No Hit ATTAACATTGGTGTCTGGGAGTGATAACAACTCATTCACTATCTCTACTC 6 0.15 No Hit GGGAAACCAAGGCAGTTGGTTGGGTAATATCAGAGCCAAGAGTGGCAGCG 6 0.15 No Hit GCCATCACTGAGACTCTCATATGCATGTTGAATTCCTTGCTGGGCATCTT 6 0.15 No Hit CGGTGACAGCGGTGACCGGATATTCTTTAGCAATGCAACCACATCACAAT 6 0.15 No Hit TTTTAGTGATACAGTCACAGGGTCATCAACAGGCATATATATTATTCACC 6 0.15 No Hit GTGGCATGCGAAGGGCCTGACCGAGTTGAGAGGCATGAGACCCTGACACA 6 0.15 No Hit CCTGACTCCTTTTCCACACCAGAATGTCAATGAAAGCAATGTGCAATACC 6 0.15 No Hit ACATCATCTCGGGATTGATTTCCTGTACAGAATAGCGATAATCAGTGTTG 6 0.15 No Hit TGCCAGTCCAGACTGTAGCATTTTTCTGCAACTTGGACATCAAGATTCAC 6 0.15 No Hit GTTGTAAGTAAGATTATCCTGCCCTTCTTTATTCTTTTTATTAACTGCCA 6 0.15 No Hit AGGAAATATACACATCGTACGGTGGTTGCATGGAACACATTGAAGTGTTC 6 0.15 No Hit GCCAGAATGATTGCTCTGATCAAGAGTTGCTGAGGAAAAGCCTTGTTCAT 6 0.15 No Hit GGTGGGTACCCACCATAACCATGTTGTGGTGGCGGCGGCTGATTATAAGG 6 0.15 No Hit GCATTGATGTCATAGAAAAAGGGTCTGGTCTTGAATAAGGGTATGATCTT 6 0.15 No Hit TGAAAATTTGGTAGGGCCACAGCTCCCCTGCCCATTAGTGCAGCAATATT 6 0.15 No Hit CTCATTTTGGCATGCGCTCTGTGTGGTTTCATGTGGCATATTTTTATCTC 5 0.125 No Hit TATGAATTTGCTCAAGAACTGGTGCACAGTTGGTATGATAAACGGTACTA 5 0.125 No Hit GTGTATGGCACTAAAGGTATTGCCATTATCGTTAAGATAAAAATCGGAAA 5 0.125 No Hit CGGTCCACAAACCTTGAATATGAACTGAAAACCTACATTGGCATGAAACA 5 0.125 No Hit GTTGTTTCCATGCCCTTGTTCACAAATTTTGTTTCACCTTTATCTGATAA 5 0.125 No Hit GCCACAGCTGTAGTTGACTGAACAAGATTCTTTTCAGCTTCAGAAGGTTT 5 0.125 No Hit ACTCATACAAACAATTAAATCAGCAAATGTATATCAGGAGGGCAGTAATT 5 0.125 No Hit GGTTGCTTTAGGAAGCGAAGCATCCTCCTTGGATTTACCAACTATATCCA 5 0.125 No Hit CCTCAAAAACCCCTCTTTTCTCTAATCCATCAATCAGCTTGCCAATCATC 5 0.125 No Hit CAACAATCTGCAACCTATCTTTAATTAACTGCACAATCACACTAGTCCCT 5 0.125 No Hit ACGAGCGTCCATGGATGTTTTATGATACTGCTAGAGAGAAATGCCATATG 5 0.125 No Hit CCATAGCCACCACCATCTAGTAACAGAACCATATCCTGCAACCTCTACTG 5 0.125 No Hit CGGCATCTGTACATCCACCTGAGACAACCAACTTAGAACCTTCTATGGTG 5 0.125 No Hit CACAGTGAGTCATAGAGGGATACAAATTTATTTGGAGGAAAGCCAACAAG 5 0.125 No Hit AGGGTTTCATTCACGTTCAAAAATAAAGTTCTTGGACGATATATCAAAGA 5 0.125 No Hit CCTGTCTCTTAAATAGTAAAGCTTGGCCCTCCTCACTTTCTTCCTGTCAT 5 0.125 No Hit GCAGTTTTCTCAGCTTGCTCAGCATTCTCCTTAATCTTCTCTAGGGCCAC 5 0.125 No Hit CGTGGAGATGGGCGGCGTAACGGTGGACTTGACCTCCCAGAGCTCGAAAC 5 0.125 No Hit ACCTGAAGAAACCAGAAAGGTGGAATTTGTGAATGGAATAGGTTGGGAAG 5 0.125 No Hit ACTGAAGTTAGATACCGATGCAGCGACTTTGCTCTTCTCAAATTTGCAAG 5 0.125 No Hit CTACTATCTTTGTAACAAACTTCTGCATCTCTTCCTTAAAAGGAGCATTA 5 0.125 No Hit CCGGGGAAAATGTATGCATTGTTTGCCTGGCCAGGCATGAAAGTTTTACC 5 0.125 No Hit AGATTGTGCATGATTGGACAGGAAGGAGCTAGCAGGTTCAGGTGAATCAG 5 0.125 No Hit CATCAATACCAGCAGCTAGGTAAACCTCAAATGGAAGGTACAAGCTGAAG 5 0.125 No Hit AGCTTCCTTGCCAAAAACAGCCCAAAGGTCACAGCTGGGTTGATGTGTCC 5 0.125 No Hit CTAGCATTTAATGAAACTCCCTGCAGCGGAGACATTAGAGAAGAATCGAG 5 0.125 No Hit CCTGTTTTCTCCTCCCCGATATACTTAATGGGAACATGAACAGCCGGGCT 5 0.125 No Hit CTCCAAAATATAATTCAGAATTCCCATGTATCAACAACAGCATCCCAAAT 5 0.125 No Hit GCCAGCTACAATCAAATTGGTTCACGAAAGGACCCGGCAGCAGAGGCTTC 5 0.125 No Hit CGGCAACCTATTATTGTAGCAGATTTAAGCGTTGCAACCCAACATTTCAA 5 0.125 No Hit GCTGTAGCCAACAAGAAATCTGTGTATTTTACAGTAGCAGAATTGATAGT 5 0.125 No Hit GCCCTCAGCTCGCTTAATCTGGATGATTTTCTCAGCTTCTGCTTTCTCAT 5 0.125 No Hit GTCGTCTTCTTCCAACCAATAAGTCTCGAGAATCTTGACTGCCTTCTCAT 5 0.125 No Hit CAGCAGCTGGTAGTGCATCCTCCTCTTCCTGTTGCTTGGCTTCTTCAGGT 5 0.125 No Hit AGGTAAACAATCTTTGCCTCCTGTTTTACATACTCAACCTCCTGAATCAC 5 0.125 No Hit CTTTGATAGTTGCACGCATGTCTCGGTAGAACAATTCCAATCTACGAAGC 5 0.125 No Hit GAATTGGTGACGCTGTGGTCGATAATTGGTGATGTAGAAGGGTTACGGGG 5 0.125 No Hit GTATGCCGCATCCAGCTTCTTGTTACCATTAGGTGTGCTAGCCCAGACGT 5 0.125 No Hit AGCATCTCTTAACTACAAACAAAAGTGCCACCACAATGCAGGAGGACTTA 5 0.125 No Hit GCATAACGCCGTAGACGTGTGATAAAATGTGAAGCTAGATCAAGAACTGC 5 0.125 No Hit CCTCAGGATAGGTTCTTTCACTAGGGTTGATTGAACTCCCAGATATATCC 5 0.125 No Hit ACCCAAACCAACATGTCCATTCCCATCACCAACAACAACAAAGGCCTTGA 5 0.125 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.025 0.0 0.0 0.0 0.0 60-61 0.025 0.0 0.0 0.0 0.0 62-63 0.025 0.0 0.0 0.0 0.0 64-65 0.025 0.0 0.0 0.0 0.0 66-67 0.025 0.0 0.0 0.0 0.0 68-69 0.025 0.0 0.0 0.0 0.0 70-71 0.037500000000000006 0.0 0.0 0.0 0.0 72-73 0.05 0.0 0.0 0.0 0.0 74-75 0.05 0.0 0.0 0.0 0.0 76-77 0.05 0.0 0.0 0.0 0.0 78-79 0.05 0.0 0.0 0.0 0.0 80-81 0.1 0.0 0.0 0.0 0.0 82-83 0.15 0.0 0.0 0.0 0.0 84-85 0.2625 0.0 0.0 0.0 0.0 86-87 0.375 0.0 0.0 0.0 0.0 88-89 0.4625 0.0 0.0 0.0 0.0 90-91 0.5 0.0 0.0 0.0 0.0 92-93 0.55 0.0 0.0 0.0 0.0 94-95 0.8125 0.0 0.0 0.0 0.0 96-97 0.95 0.0 0.0 0.0 0.0 98-99 1.2625 0.0 0.0 0.0 0.0 100-101 1.4375 0.0 0.0 0.0 0.0 102-103 1.7125 0.0 0.0 0.0 0.0 104-105 2.075 0.0 0.0 0.0 0.0 106-107 2.3625 0.0 0.0 0.0 0.0 108-109 2.625 0.0 0.0 0.0 0.0 110-111 2.8499999999999996 0.0 0.0 0.0 0.0 112-113 3.125 0.0 0.0 0.0 0.0 114-115 3.7125000000000004 0.0 0.0 0.0 0.0 116-117 4.1625 0.0 0.0 0.0 0.0 118-119 4.5625 0.0 0.0 0.0 0.0 120-121 4.9 0.0 0.0 0.0 0.0 122-123 5.5375 0.0 0.0 0.0 0.0 124-125 6.0625 0.0 0.0 0.0 0.0 126-127 6.75 0.0 0.0 0.0 0.0 128-129 7.225 0.0 0.0 0.0 0.0 130-131 7.9875 0.0 0.0 0.0 0.0 132-133 8.475 0.0 0.0 0.0 0.0 134-135 9.087499999999999 0.0 0.0 0.0 0.0 136-137 9.5875 0.0 0.0 0.0 0.0 138-139 10.1 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGAGATA 10 0.006830828 145.0 4 AGCTAGA 10 0.006830828 145.0 145 GATAAAA 10 0.006830828 145.0 7 ATAAAAG 10 0.006830828 145.0 8 AAGGAGA 10 0.006830828 145.0 2 GAGATAA 10 0.006830828 145.0 5 AGGAGAT 10 0.006830828 145.0 3 >>END_MODULE SRR26075392 read2 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR26075392_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.168 37.0 37.0 37.0 37.0 37.0 2 36.2085 37.0 37.0 37.0 37.0 37.0 3 36.1525 37.0 37.0 37.0 37.0 37.0 4 36.2425 37.0 37.0 37.0 37.0 37.0 5 36.3045 37.0 37.0 37.0 37.0 37.0 6 36.13 37.0 37.0 37.0 37.0 37.0 7 36.107 37.0 37.0 37.0 37.0 37.0 8 36.2865 37.0 37.0 37.0 37.0 37.0 9 36.277 37.0 37.0 37.0 37.0 37.0 10-14 36.1694 37.0 37.0 37.0 37.0 37.0 15-19 36.150800000000004 37.0 37.0 37.0 37.0 37.0 20-24 36.1995 37.0 37.0 37.0 37.0 37.0 25-29 36.0929 37.0 37.0 37.0 37.0 37.0 30-34 35.9844 37.0 37.0 37.0 37.0 37.0 35-39 35.9276 37.0 37.0 37.0 37.0 37.0 40-44 35.9495 37.0 37.0 37.0 37.0 37.0 45-49 35.866 37.0 37.0 37.0 37.0 37.0 50-54 35.7177 37.0 37.0 37.0 37.0 37.0 55-59 35.7321 37.0 37.0 37.0 37.0 37.0 60-64 35.8015 37.0 37.0 37.0 37.0 37.0 65-69 35.7634 37.0 37.0 37.0 37.0 37.0 70-74 35.5861 37.0 37.0 37.0 37.0 37.0 75-79 35.4894 37.0 37.0 37.0 37.0 37.0 80-84 35.6546 37.0 37.0 37.0 37.0 37.0 85-89 35.6255 37.0 37.0 37.0 37.0 37.0 90-94 35.3869 37.0 37.0 37.0 37.0 37.0 95-99 35.482899999999994 37.0 37.0 37.0 37.0 37.0 100-104 35.291900000000005 37.0 37.0 37.0 34.6 37.0 105-109 35.3464 37.0 37.0 37.0 34.6 37.0 110-114 35.269 37.0 37.0 37.0 32.2 37.0 115-119 35.2779 37.0 37.0 37.0 34.6 37.0 120-124 35.0163 37.0 37.0 37.0 27.4 37.0 125-129 35.09740000000001 37.0 37.0 37.0 25.0 37.0 130-134 35.0441 37.0 37.0 37.0 27.4 37.0 135-139 34.833999999999996 37.0 37.0 37.0 25.0 37.0 140-144 34.8693 37.0 37.0 37.0 25.0 37.0 145-149 34.7425 37.0 37.0 37.0 25.0 37.0 150-151 34.38875 37.0 37.0 37.0 25.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.0 1101 2 0.0 1101 3 0.0 1101 4 0.0 1101 5 0.0 1101 6 0.0 1101 7 0.0 1101 8 0.0 1101 9 0.0 1101 10-14 0.0 1101 15-19 0.0 1101 20-24 0.0 1101 25-29 0.0 1101 30-34 0.0 1101 35-39 0.0 1101 40-44 0.0 1101 45-49 0.0 1101 50-54 0.0 1101 55-59 0.0 1101 60-64 0.0 1101 65-69 0.0 1101 70-74 0.0 1101 75-79 0.0 1101 80-84 0.0 1101 85-89 0.0 1101 90-94 0.0 1101 95-99 0.0 1101 100-104 0.0 1101 105-109 0.0 1101 110-114 0.0 1101 115-119 0.0 1101 120-124 0.0 1101 125-129 0.0 1101 130-134 0.0 1101 135-139 0.0 1101 140-144 0.0 1101 145-149 0.0 1101 150-151 0.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 13 1.0 14 0.0 15 7.0 16 3.0 17 4.0 18 7.0 19 3.0 20 8.0 21 8.0 22 5.0 23 10.0 24 8.0 25 12.0 26 13.0 27 15.0 28 18.0 29 21.0 30 27.0 31 46.0 32 67.0 33 99.0 34 244.0 35 792.0 36 2437.0 37 145.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.675 23.724999999999998 10.424999999999999 20.175 2 27.525 26.825 25.074999999999996 20.575 3 22.0 27.800000000000004 31.374999999999996 18.825 4 26.700000000000003 32.225 24.099999999999998 16.975 5 27.3 33.7 21.375 17.625 6 22.925 37.3 21.6 18.175 7 21.025 23.400000000000002 36.1 19.475 8 23.674999999999997 25.224999999999998 26.424999999999997 24.675 9 23.075000000000003 26.5 29.299999999999997 21.125 10-14 24.529999999999998 28.665000000000003 25.724999999999998 21.08 15-19 24.42 28.655 25.715 21.21 20-24 23.799999999999997 27.525 27.169999999999998 21.505 25-29 23.91 28.084999999999997 26.974999999999998 21.029999999999998 30-34 24.395 29.09 26.41 20.105 35-39 24.16 28.34 26.685 20.815 40-44 24.95 28.21 26.35 20.49 45-49 24.29 28.439999999999998 26.005 21.265 50-54 23.97 27.865000000000002 28.28 19.885 55-59 24.275 28.044999999999998 26.924999999999997 20.755000000000003 60-64 25.169999999999998 28.144999999999996 25.66 21.025 65-69 24.095 28.315 26.56 21.029999999999998 70-74 24.94 28.415000000000003 26.56 20.085 75-79 24.985 28.199999999999996 26.815 20.0 80-84 24.465 27.6 27.47 20.465 85-89 24.38 28.215 27.169999999999998 20.235 90-94 24.015 28.84 26.840000000000003 20.305 95-99 24.044999999999998 27.800000000000004 27.815 20.34 100-104 24.845 28.110000000000003 26.179999999999996 20.865000000000002 105-109 25.355 28.18 26.195 20.27 110-114 24.98 29.165000000000003 25.869999999999997 19.985 115-119 24.55 28.53 26.775 20.145 120-124 24.060000000000002 28.455000000000002 26.735 20.75 125-129 25.0 28.410000000000004 26.650000000000002 19.939999999999998 130-134 25.905 27.605 26.72 19.77 135-139 26.445 27.810000000000002 25.790000000000003 19.955000000000002 140-144 26.32 27.48 26.47 19.73 145-149 26.939999999999998 27.325 26.979999999999997 18.755 150-151 26.900000000000002 28.8625 26.1125 18.125 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.5 8 0.5 9 0.0 10 0.0 11 0.5 12 1.5 13 2.0 14 2.0 15 1.0 16 1.5 17 2.0 18 1.0 19 1.0 20 1.0 21 0.5 22 1.5 23 2.5 24 2.0 25 2.0 26 4.0 27 4.5 28 6.5 29 7.0 30 9.0 31 10.5 32 9.0 33 16.0 34 24.0 35 42.5 36 65.0 37 83.5 38 109.0 39 149.0 40 206.5 41 252.5 42 243.5 43 238.5 44 273.0 45 282.0 46 265.0 47 265.0 48 270.5 49 236.5 50 181.0 51 150.5 52 124.5 53 97.5 54 75.5 55 49.0 56 36.5 57 34.0 58 34.5 59 30.0 60 19.5 61 10.5 62 6.5 63 7.5 64 6.5 65 4.0 66 2.5 67 1.0 68 1.5 69 1.0 70 0.0 71 0.0 72 0.5 73 1.5 74 1.0 75 0.5 76 0.5 77 0.0 78 0.5 79 0.5 80 1.0 81 1.0 82 0.5 83 1.0 84 0.5 85 0.5 86 0.5 87 0.5 88 1.0 89 0.5 90 0.5 91 0.5 92 1.0 93 2.5 94 2.0 95 1.0 96 1.5 97 1.0 98 0.5 99 1.0 100 4.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150-151 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 59.52499999999999 #Duplication Level Percentage of deduplicated Percentage of total 1 63.628727425451494 37.875 2 19.781604367912642 23.549999999999997 3 8.81982360352793 15.75 4 4.199916001679966 10.0 5 1.7219655606887863 5.125 6 0.9239815203695927 3.3000000000000003 7 0.629987400251995 2.625 8 0.125997480050399 0.6 9 0.0 0.0 >10 0.16799664006719867 1.175 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG 16 0.4 No Hit GAGTACAAATCGATATCCTCATTTCTCTACCATTTTTTAAGGAGTAGAGA 11 0.27499999999999997 No Hit CTGGCTAATTCAGACCAACCTATTGATTCCACCGCTAATGTTTCCTATGC 10 0.25 No Hit GGAGCAGACCCAGAGAAAAATCAACCTGGTGATCTCTATGTGACAATCAA 10 0.25 No Hit GTCGAGGGCACACCAGGAATCAAGAAACTTGATTGGCATGAGGATTCTGA 8 0.2 No Hit GCAAGAAGATTGATACAGTGAACAAAGAATTGAAGCCATTAGGACATACT 8 0.2 No Hit GCGTTACTTCCACAAGCTCCGCAACAAGTTCCACTGTCCGATCGTGAACA 8 0.2 No Hit ATCTAACGCGTCGAAACCCTAATCACCGGATAAGGTCTGAGCTTTCAGGT 7 0.17500000000000002 No Hit GGAGCCTATCAGCATCACAGTCCAATAATGGCGATGTGGTTTTGAGAATG 7 0.17500000000000002 No Hit AAGCAAAGGACGTAACAAGGAGGGGCTTGAATATATATATATATATCAAC 7 0.17500000000000002 No Hit GACAAGTGCAAGTATAACATCTTGTCCACCAAAGTTGAATTTCGACTTGC 7 0.17500000000000002 No Hit GAAGGCTCAACCGCTAACCGCCGTAGCTAAAAACGATTAACTTCCCTCCC 7 0.17500000000000002 No Hit TAATAACTGGAAAAATCAGCCAATGCCTAATTCCGCCGAAATGAGGCGGC 7 0.17500000000000002 No Hit TCTTGATTGACCCATCAGTTGAGCAACTAATTATCAAGGACTAGCAGAAG 7 0.17500000000000002 No Hit CCGGATTTACCTGACCCCCCAGTTCTGAACCATTTCATCGATGTCTTTTT 7 0.17500000000000002 No Hit TCCTATAGAAGCAACATCAGCAGCTAATTCTGCAAGACCTTCAATCGAGA 7 0.17500000000000002 No Hit GATGGGCATCGTTTAAGGGTGGAACTTGCACATGGTGGGCGTGGACATTC 7 0.17500000000000002 No Hit GCACCTTACCTAGTGATGAGGTTGAGAGGATGGTCAGCGAAGCTGATAAG 7 0.17500000000000002 No Hit GGGAAAAGAAGTGGAAATGCGATAAATGTTCGAAGAAGTATGCTGTTCAG 7 0.17500000000000002 No Hit ATATGGGATCCGCAGAGACCCATGAATGTGATGGAGAGACGGTCAAGAAG 7 0.17500000000000002 No Hit GTGTATAATTAGAGGAGATGATAAACTGGGACTAGGAGTTAATGTAACAA 7 0.17500000000000002 No Hit ATTTGGGGAATAGTGGAGATGCTAATTTCTATGCCCAGATGATAGATGAT 7 0.17500000000000002 No Hit ACATCATCGACGTGGGCCCCGCTATCGAACGCTGATTTCAACCGTACAAT 6 0.15 No Hit CTCCACTCTGTACCCGTCTCCACCAGCAAACAGGGCCAACAACATGCTGG 6 0.15 No Hit CAGCATCAGTGGAAACTGCGAAGAAGGAGGATGTTGTCTCATCACCGGCA 6 0.15 No Hit GAAATTTGCTCCGATCCTATGCTATCAACCAGAAATTTCAAGAATGGTAT 6 0.15 No Hit GCTGTGAGGTGGTGAAATTGCTTCTCGATGCAGATGCTGCGATTGTGATG 6 0.15 No Hit CTCGCTATCTGCCATACTGCAATACCTGAAATTGATGAAGAAACTGGAAG 6 0.15 No Hit AAGTCAGATGAGGAAATTAAAACAATGACAACTTCAGTTGCAGAGTTGGC 6 0.15 No Hit GCAGAACATGAAGGTTAAGCTGATTGTATTGGGTATACTGATTGTCTTGA 6 0.15 No Hit GCCAGCTGGTCCAGCCCCTTATTTTCACAAGGATTGCATATCGAATATCC 6 0.15 No Hit AGTAAACTATGGACACCACGAGATACCCCAGTGGCTCCTGCAATATTCAA 6 0.15 No Hit ATATCGTCCACGCCGAGTGGAGGTGAGGTTGCGGCGACGGTGCAGAGGCC 6 0.15 No Hit GAAAGTCGAAATTTGAAATTTTGGGAGAGAGATGGCAACAGCATCAAGCA 6 0.15 No Hit TGGAAAGATCTTTTGATTGCTGTGTTTATGCAGACACCTGCCGATATGGA 6 0.15 No Hit GCTTGTACACGTCATATCAGCATGATTATTTTCTATCAATTTGAGAAACT 6 0.15 No Hit GCCGGCGGGAAGGAAATGAACAAGCCTGTCACTGCCTACCCTGTGATGGG 6 0.15 No Hit GACATGAGAGAGTGCTCGCTAGTGAAGCTGCCAAATTCTGTTGCATCATT 6 0.15 No Hit CATCAGGCGGTGGGAAGTGTCAAGCGCTTCTTTGCACGGCAGATATTAAT 6 0.15 No Hit GGGTTATTTGGACGTGTTTATCGAGCTGAGTTTGATGATGGGAAGGTTGT 6 0.15 No Hit TATGTATCCCAATTCGAGCATACCATCCTACTGCGGCCAACCTGCAAAGA 6 0.15 No Hit GCCCATTCTATTTTCAACAGTTTAATGGACGAGGGACACAAGCCAACTCT 6 0.15 No Hit GGTACAAGTGCATTTCTAAAAAGTATATCGCTTGAAGCTGTTGGATTTGG 6 0.15 No Hit GAAGAGGTGGTTGAGATTGTATCCTCAGCTCCAACACGGGCATCAGCAGC 6 0.15 No Hit GAGGAGGGTGTTGGACCCAGCTCAGAGATCCTGGTGAAAATTGCAGACAG 5 0.125 No Hit GGGATATCCGGATGATTTACTCAAAAGACAATGAGGGACTGCTTTGTGTT 5 0.125 No Hit GGAGACATAGTTTCTGCCATTATGGAGCTTACCAATTGATAGGTTCGTAT 5 0.125 No Hit AGTGGATTTGGGCTTTGAGGATGACATAAGGGAAGTTTTCGACCACTTCA 5 0.125 No Hit GGAGAAGCAAGGCAGTTTAGGACATCTCTGCAGTATGAAAACTCCATATC 5 0.125 No Hit GTGCGACTCAAAGTGGAAGTGCCGGAGAATAAGCGGCGTGTTTCGACTAT 5 0.125 No Hit CCATGGCAGGCTTCTGGCGAGACATTATCTGGCATTGATCCTGAAGCAAT 5 0.125 No Hit ACAAGGGGCACATAAATCAGGATAACAATTTCTTGCTCATTTTTCTAGGA 5 0.125 No Hit AGTAGTTGCAGACAGAGAGCTAGTGTTAGACAGGTTCTTGTTAGACAGAG 5 0.125 No Hit GCAAGATTTGCGACTGTCCTCCCTGTATAGTACCGGAAAGCATCAAGGAT 5 0.125 No Hit TTTGGATTGGTACTGGAACTGTTATTGGATTAATTATCCTAGCCATCACC 5 0.125 No Hit AGAAGAAATAATCAACAGGATAGTGATCCAGCTCATATGCAGGTGGAGGA 5 0.125 No Hit CGAGTTGTTCCTGCGCCGAATCGAGAAGGAGCGTATCAGAGAGGAAATAA 5 0.125 No Hit AGATTGCCCACATGTACAAGACTGACAGGAGCAAATACGAGACAACTGCA 5 0.125 No Hit GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGT 5 0.125 No Hit AGATATTTCAAGTGCTGCCTTATTGGTGTGCTCACTCTTGTTCACAACTC 5 0.125 No Hit TGATGATGATGAAGCAAAGCTAGCAATCTCCGAGTTGAGGAAGGGTGTCT 5 0.125 No Hit AATTCTCTCTCTTTCCAACCCAACTTCACAATCTGAATGTACCGCTGAAG 5 0.125 No Hit AACGAAATAGCAAGAGCTGTTGAAGAGGAGCTTGAAAAGGCCATGTCTGC 5 0.125 No Hit GAGAGAGTAGAAGGACACCAAAGCACGCAACTAGTGTTCATTCCAAGAAA 5 0.125 No Hit CAAACCCCTCTCCCCAGTCTCCGCCACTCTTCGTTCCTCTTAAACCCTAA 5 0.125 No Hit CGGACAAATCCGATCGCCGATCTCTCCTTCTCTCCGATTTTCCGGGTTCA 5 0.125 No Hit GAACATTTAGAAATTTTTGCTGCATTGAAGGGTGTGAAGGAAGATATTTT 5 0.125 No Hit GTTACTCAAGCCAACTCCAGCAACAGCAACAGCAACTAAGGGATGAGATG 5 0.125 No Hit TTTGAATGAGCTGAACAGAGGACCTAGGGCCAAGGGATATTTCAAAAACC 5 0.125 No Hit CTTGATGCTGCAAATCCAGTGTGGCAGCGGATAAGTGTGAAATCATCCCC 5 0.125 No Hit TGTGGATTGTGATCACGAGTGTGATCCAGAAGAATATGGTGCCTGTGACT 5 0.125 No Hit AACAAAGGCAAGCACAGCATCGGATGCCTGTTTTGGCTGTTGGCAAGAAT 5 0.125 No Hit TGAGTTGATGAGTGGAATTGGTGGTCAAGTGAAAGGAGGGGAGGCAAAAG 5 0.125 No Hit GGTTCGACTGCTAATGACTTCGTAGATGATTGGGTCAAAATCGGTTTACC 5 0.125 No Hit CCTGTGTTTATGACCTTGTATTTTGAGGATCCTGATCATCAGGGTCATAA 5 0.125 No Hit CCTGGCTATGAGCACTTGAATGATCCACTTCACATCTTAATCGAGGCCGA 5 0.125 No Hit AGGAGGAAGAGAAATGGGTACCAGTCACAAAACTAGGCCGCCTTGTAAAA 5 0.125 No Hit AAGGAACCTCTGGCATTGACAACAAGTTTACAACTGTGCAATTTACTGGA 5 0.125 No Hit GCAGCCATGCCTCCTTTCCTCCTCTTGATCTCTCATTCCACCAATACAGC 5 0.125 No Hit GTTACTAGATCATCAGGTCCCTGTGCAATCTTCAATTTTCATATAGTTCG 5 0.125 No Hit AGTGTTTGTACCGAAGAAGAAATCTGGTATCCGTTGGTACTCCAAATACC 5 0.125 No Hit AGTTAAAGGACAGTATTACTTTGAAGGGAGATATGATCTTGTAAAGTTTG 5 0.125 No Hit ATGCGATGTCCGTGCCTATGGCGATAGCACTCTCTGGACAGCCCCTTCTT 5 0.125 No Hit GGCAGACCTTTTACTTACCAAGATATCCCAATCTCATTGCAGAATTCAGG 5 0.125 No Hit AAGAGGCTCAACATTGATCATCAATATGCCTGGGAATCCTAATGCGGTTG 5 0.125 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.025 0.0 0.0 0.0 0.0 60-61 0.025 0.0 0.0 0.0 0.0 62-63 0.025 0.0 0.0 0.0 0.0 64-65 0.025 0.0 0.0 0.0 0.0 66-67 0.025 0.0 0.0 0.0 0.0 68-69 0.025 0.0 0.0 0.0 0.0 70-71 0.037500000000000006 0.0 0.0 0.0 0.0 72-73 0.05 0.0 0.0 0.0 0.0 74-75 0.05 0.0 0.0 0.0 0.0 76-77 0.05 0.0 0.0 0.0 0.0 78-79 0.05 0.0 0.0 0.0 0.0 80-81 0.1 0.0 0.0 0.0 0.0 82-83 0.15 0.0 0.0 0.0 0.0 84-85 0.2625 0.0 0.0 0.0 0.0 86-87 0.3875 0.0 0.0 0.0 0.0 88-89 0.4875 0.0 0.0 0.0 0.0 90-91 0.525 0.0 0.0 0.0 0.0 92-93 0.575 0.0 0.0 0.0 0.0 94-95 0.8375 0.0 0.0 0.0 0.0 96-97 1.025 0.0 0.0 0.0 0.0 98-99 1.3375 0.0 0.0 0.0 0.0 100-101 1.5125 0.0 0.0 0.0 0.0 102-103 1.8 0.0 0.0 0.0 0.0 104-105 2.175 0.0 0.0 0.0 0.0 106-107 2.4625 0.0 0.0 0.0 0.0 108-109 2.725 0.0 0.0 0.0 0.0 110-111 2.95 0.0 0.0 0.0 0.0 112-113 3.2375 0.0 0.0 0.0 0.0 114-115 3.85 0.0 0.0 0.0 0.0 116-117 4.325 0.0 0.0 0.0 0.0 118-119 4.725 0.0 0.0 0.0 0.0 120-121 5.0375 0.0 0.0 0.0 0.0 122-123 5.65 0.0 0.0 0.0 0.0 124-125 6.1625 0.0 0.0 0.0 0.0 126-127 6.8125 0.0 0.0 0.0 0.0 128-129 7.275 0.0 0.0 0.0 0.0 130-131 8.175 0.0 0.0 0.0 0.0 132-133 8.712499999999999 0.0 0.0 0.0 0.0 134-135 9.337499999999999 0.0 0.0 0.0 0.0 136-137 9.899999999999999 0.0 0.0 0.0 0.0 138-139 10.412500000000001 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTGAGC 10 0.006830828 145.0 5 AGCTACA 10 0.006830828 145.0 9 GAGCTAC 10 0.006830828 145.0 8 GTGAGCT 10 0.006830828 145.0 6 TGGTGAG 10 0.006830828 145.0 4 TGAGCTA 10 0.006830828 145.0 7 >>END_MODULE Read 825240 spots for SRR26075392.sra Written 825240 spots for SRR26075392.sra Read 825240 spots for SRR26075392.sra Written 825240 spots for SRR26075392.sra Read 825240 spots for SRR26075392.sra Written 825240 spots for SRR26075392.sra Read 825240 spots for SRR26075392.sra Written 825240 spots for SRR26075392.sra Read 825240 spots for SRR26075392.sra Written 825240 spots for SRR26075392.sra Read 825240 spots for SRR26075392.sra Written 825240 spots for SRR26075392.sra Read 825240 spots for SRR26075392.sra Written 825240 spots for SRR26075392.sra Read 825240 spots for SRR26075392.sra Written 825240 spots for SRR26075392.sra Read 825240 spots for SRR26075392.sra Written 825240 spots for SRR26075392.sra Read 825240 spots for SRR26075392.sra Written 825240 spots for SRR26075392.sra Read 825240 spots for SRR26075392.sra Written 825240 spots for SRR26075392.sra Read 825240 spots for SRR26075392.sra Written 825240 spots for SRR26075392.sra Read 825240 spots for SRR26075392.sra Written 825240 spots for SRR26075392.sra Read 825240 spots for SRR26075392.sra Written 825240 spots for SRR26075392.sra Read 825240 spots for SRR26075392.sra Written 825240 spots for SRR26075392.sra Read 825240 spots for SRR26075392.sra Written 825240 spots for SRR26075392.sra Read 825240 spots for SRR26075392.sra Written 825240 spots for SRR26075392.sra Read 825240 spots for SRR26075392.sra Written 825240 spots for SRR26075392.sra Read 825243 spots for SRR26075392.sra Written 825243 spots for SRR26075392.sra Read 825240 spots for SRR26075392.sra Written 825240 spots for SRR26075392.sra SRR ids: ['SRR26075392.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_xvfy67ws SRR26075392.sra spots: 16504803 blocks: [[1, 825240], [825241, 1650480], [1650481, 2475720], [2475721, 3300960], [3300961, 4126200], [4126201, 4951440], [4951441, 5776680], [5776681, 6601920], [6601921, 7427160], [7427161, 8252400], [8252401, 9077640], [9077641, 9902880], [9902881, 10728120], [10728121, 11553360], [11553361, 12378600], [12378601, 13203840], [13203841, 14029080], [14029081, 14854320], [14854321, 15679560], [15679561, 16504803]] SRR26075392 file size 6089251 SRR26075392 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o SRR26075392 SRR26075392_1.fastq SRR26075392_2.fastq Input file: SRR26075392_1.fastq Paired file: SRR26075392_2.fastq trimmed: SRR26075392-trimmed-pair1.fastq, SRR26075392-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Wed Feb 12 03:51:27 2025 >> started Wed Feb 12 03:51:55 2025 >> done (28.151s) 16504803 read pairs processed; of these: 95 ( 0.00%) short read pairs filtered out after trimming by size control 25806 ( 0.16%) empty read pairs filtered out after trimming by size control 16478902 (99.84%) read pairs available; of these: 2506583 (15.21%) trimmed read pairs available after processing 13972319 (84.79%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 18 7 0.00% 19 12 0.00% 20 8 0.00% 21 13 0.00% 22 17 0.00% 23 23 0.00% 24 13 0.00% 25 19 0.00% 26 10 0.00% 27 14 0.00% 28 23 0.00% 29 20 0.00% 30 20 0.00% 31 19 0.00% 32 20 0.00% 33 20 0.00% 34 23 0.00% 35 27 0.00% 36 29 0.00% 37 29 0.00% 38 39 0.00% 39 24 0.00% 40 34 0.00% 41 27 0.00% 42 45 0.00% 43 58 0.00% 44 36 0.00% 45 62 0.00% 46 56 0.00% 47 82 0.00% 48 71 0.00% 49 83 0.00% 50 77 0.00% 51 89 0.00% 52 118 0.00% 53 93 0.00% 54 122 0.00% 55 157 0.00% 56 146 0.00% 57 187 0.00% 58 243 0.00% 59 247 0.00% 60 266 0.00% 61 310 0.00% 62 375 0.00% 63 424 0.00% 64 452 0.00% 65 538 0.00% 66 510 0.00% 67 661 0.00% 68 730 0.00% 69 775 0.00% 70 936 0.01% 71 1306 0.01% 72 1306 0.01% 73 1554 0.01% 74 1664 0.01% 75 1979 0.01% 76 2141 0.01% 77 2373 0.01% 78 2742 0.02% 79 3003 0.02% 80 3475 0.02% 81 3966 0.02% 82 4394 0.03% 83 5030 0.03% 84 5525 0.03% 85 6204 0.04% 86 7037 0.04% 87 7310 0.04% 88 7613 0.05% 89 8325 0.05% 90 9356 0.06% 91 9733 0.06% 92 10804 0.07% 93 11972 0.07% 94 12796 0.08% 95 13878 0.08% 96 15022 0.09% 97 15791 0.10% 98 15970 0.10% 99 16973 0.10% 100 17844 0.11% 101 18873 0.11% 102 20203 0.12% 103 21826 0.13% 104 22950 0.14% 105 23772 0.14% 106 25593 0.16% 107 26449 0.16% 108 26700 0.16% 109 27600 0.17% 110 28448 0.17% 111 29272 0.18% 112 30296 0.18% 113 31836 0.19% 114 33519 0.20% 115 35253 0.21% 116 36381 0.22% 117 37287 0.23% 118 38368 0.23% 119 38620 0.23% 120 38905 0.24% 121 39786 0.24% 122 40534 0.25% 123 42560 0.26% 124 44641 0.27% 125 44809 0.27% 126 47383 0.29% 127 47809 0.29% 128 48670 0.30% 129 49706 0.30% 130 50433 0.31% 131 50606 0.31% 132 51590 0.31% 133 53883 0.33% 134 54809 0.33% 135 56252 0.34% 136 57532 0.35% 137 58240 0.35% 138 58952 0.36% 139 60288 0.37% 140 60136 0.36% 141 60866 0.37% 142 61641 0.37% 143 62823 0.38% 144 64427 0.39% 145 66188 0.40% 146 67654 0.41% 147 68647 0.42% 148 69247 0.42% 149 68493 0.42% 150 70302 0.43% 151 13972319 84.79% 16478902 reads passed initial QC criterion=sequence-density sequence-density=0.23 sequence-density-rank=1 fanout-score=5.23 fanout-score-rank=29 prefix-density=0.33 prefix-fanout=3.6 sequence=TCCACACTTGCAGCCATTCTC criterion=fanout-score sequence-density=0.03 sequence-density-rank=35 fanout-score=107.77 fanout-score-rank=1 prefix-density=0.34 prefix-fanout=9.8 sequence=CATCACCATCCCTGATTGATCTTTGATTCACAACAAGACCAACCAACCGTACAATGTTTACATAACTTGGGATAATCTCACAAGAAAGAAAGATCAATACAAAACTAGTCAGCTCAGGATGTTCCAGGCACGGTAGAACCGTAGAACCATAACAACAAGAGACATATTGCAGATGAGTACTGAAAAACAAAACACAGTACGTATTTACATGGGCAACCTTGGTTGAAGGCAACCTCATCAACGATGCTCGCTCTTCGTCTCTCCACTGTACATCCAGTCATAGACAGTGGGTGTGTTAGGCTGTGGCTTGTCAAAAACATGAGCACCAATATTCTTAGTAGCAAGGTTGCTACCAGGGTGGAACACGCTCCTCCAAACATTGCTACGCGCCGACACTGGGGTTGTAGGGGTCACTGGTGTCGTCGGTGTCCCTGGAGTTCCTGGCATAGTCATGGACCTCTGAAACTTATTAACAGGACTGCTCCCCTCTCCGACGTCAATATCTTTGATG criterion=sequence-density sequence-density=0.42 sequence-density-rank=1 fanout-score=2.46 fanout-score-rank=33 prefix-density=0.42 prefix-fanout=2.4 sequence=ATGTACCCTGAC criterion=fanout-score sequence-density=0.09 sequence-density-rank=21 fanout-score=373.74 fanout-score-rank=1 prefix-density=1.02 prefix-fanout=33.9 sequence=AAGAAGAAGAAA SRR26075392 testing PE reads STAR mapping to Ensembl genome Started job on | Feb 12 03:52:34 Started mapping on | Feb 12 03:52:34 Finished on | Feb 12 03:54:52 Mapping speed, Million of reads per hour | 429.88 Number of input reads | 16478902 Average input read length | 294 UNIQUE READS: Uniquely mapped reads number | 15036120 Uniquely mapped reads % | 91.24% Average mapped length | 292.85 Number of splices: Total | 14277803 Number of splices: Annotated (sjdb) | 13927320 Number of splices: GT/AG | 14002268 Number of splices: GC/AG | 213702 Number of splices: AT/AC | 16272 Number of splices: Non-canonical | 45561 Mismatch rate per base, % | 0.40% Deletion rate per base | 0.03% Deletion average length | 3.04 Insertion rate per base | 0.02% Insertion average length | 2.57 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 386495 % of reads mapped to multiple loci | 2.35% Number of reads mapped to too many loci | 43913 % of reads mapped to too many loci | 0.27% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 5.86% % of reads unmapped: other | 0.28% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 1056287 1056287 1056287 N_multimapping 386495 386495 386495 N_noFeature 391264 14876506 481026 N_ambiguous 153228 1040 82611 UnstrandedReadsAssigned:14491628 PositiveStrandReadsAssigned:158574 NegativeStrandReadsAssigned:14472483 Dataset is classified negative stranded MeadianReadLen=151 20thPercentileLength=151 echo kmer=147 SRR26075392 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,400 [index] number of k-mers: 62,057,036 [index] number of equivalence classes: 130,681 [quant] running in paired-end mode [quant] will process pair 1: SRR26075392-trimmed-pair1.fastq SRR26075392-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 16,478,902 reads, 14,664,232 reads pseudoaligned [quant] estimated average fragment length: 217.251 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,213 rounds 52401 SRR26075392.ke.tsv 34699 SRR26075392.se.tsv 87100 total ==> SRR26075392.ke.tsv <== target_id length eff_length est_counts tpm Potri.005G200100.1.v4.1 2018 1801.75 1415 52.5675 Potri.005G024800.1.v4.1 1035 818.749 1361 111.266 Potri.004G059700.1.v4.1 961 744.749 1 0.0898763 Potri.007G009000.2.v4.1 1416 1199.75 0 0 Potri.003G141000.2.v4.1 2943 2726.75 838 20.5709 Potri.016G087400.1.v4.1 270 89.4957 837 626.006 Potri.015G069301.1.v4.1 564 350.072 0 0 Potri.010G195200.1.v4.1 1773 1556.75 727 31.2587 Potri.012G127500.1.v4.1 977 760.749 38536 3390.63 ==> SRR26075392.se.tsv <== Potri.001G166300.v4.1 0 Potri.001G448400.v4.1 21 Potri.001G233950.v4.1 0 Potri.001G122700.v4.1 169 Potri.001G212900.v4.1 0 Potri.001G182400.v4.1 0 Potri.001G256600.v4.1 0 Potri.001G040500.v4.1 0 Potri.001G416900.v4.1 0 Potri.001G452600.v4.1 576 SRR26075392 completed mapping pipeline successfully