Starting /dee2/code/volunteer_pipeline.sh SRR26075393 current disk space = 3051397836800 free memory = 1472348220 SRR26075393 SRAfilesize e6bf3047756a5c270d05108c0af68b96 SRR26075393.sra SRR26075393.sra file validated SRR26075393 is paired end SRR26075393 is conventional basespace SRR26075393 read1 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR26075393_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.6265 37.0 37.0 37.0 37.0 37.0 2 36.6025 37.0 37.0 37.0 37.0 37.0 3 36.6215 37.0 37.0 37.0 37.0 37.0 4 36.7015 37.0 37.0 37.0 37.0 37.0 5 36.7045 37.0 37.0 37.0 37.0 37.0 6 36.709 37.0 37.0 37.0 37.0 37.0 7 36.603 37.0 37.0 37.0 37.0 37.0 8 36.582 37.0 37.0 37.0 37.0 37.0 9 36.653 37.0 37.0 37.0 37.0 37.0 10-14 36.64535 37.0 37.0 37.0 37.0 37.0 15-19 36.598699999999994 37.0 37.0 37.0 37.0 37.0 20-24 36.561 37.0 37.0 37.0 37.0 37.0 25-29 36.4481 37.0 37.0 37.0 37.0 37.0 30-34 36.4373 37.0 37.0 37.0 37.0 37.0 35-39 36.3806 37.0 37.0 37.0 37.0 37.0 40-44 36.3417 37.0 37.0 37.0 37.0 37.0 45-49 36.2528 37.0 37.0 37.0 37.0 37.0 50-54 36.2124 37.0 37.0 37.0 37.0 37.0 55-59 36.210899999999995 37.0 37.0 37.0 37.0 37.0 60-64 36.1241 37.0 37.0 37.0 37.0 37.0 65-69 36.0895 37.0 37.0 37.0 37.0 37.0 70-74 36.0563 37.0 37.0 37.0 37.0 37.0 75-79 36.0452 37.0 37.0 37.0 37.0 37.0 80-84 35.976 37.0 37.0 37.0 37.0 37.0 85-89 35.9303 37.0 37.0 37.0 37.0 37.0 90-94 35.8697 37.0 37.0 37.0 37.0 37.0 95-99 35.80550000000001 37.0 37.0 37.0 37.0 37.0 100-104 35.80179999999999 37.0 37.0 37.0 37.0 37.0 105-109 35.7787 37.0 37.0 37.0 37.0 37.0 110-114 35.6513 37.0 37.0 37.0 37.0 37.0 115-119 35.6049 37.0 37.0 37.0 37.0 37.0 120-124 35.660399999999996 37.0 37.0 37.0 37.0 37.0 125-129 35.5492 37.0 37.0 37.0 37.0 37.0 130-134 35.3947 37.0 37.0 37.0 34.6 37.0 135-139 35.248900000000006 37.0 37.0 37.0 32.2 37.0 140-144 35.165499999999994 37.0 37.0 37.0 27.4 37.0 145-149 35.1151 37.0 37.0 37.0 27.4 37.0 150-151 34.917 37.0 37.0 37.0 25.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.0 1101 2 0.0 1101 3 0.0 1101 4 0.0 1101 5 0.0 1101 6 0.0 1101 7 0.0 1101 8 0.0 1101 9 0.0 1101 10-14 0.0 1101 15-19 0.0 1101 20-24 0.0 1101 25-29 0.0 1101 30-34 0.0 1101 35-39 0.0 1101 40-44 0.0 1101 45-49 0.0 1101 50-54 0.0 1101 55-59 0.0 1101 60-64 0.0 1101 65-69 0.0 1101 70-74 0.0 1101 75-79 0.0 1101 80-84 0.0 1101 85-89 0.0 1101 90-94 0.0 1101 95-99 0.0 1101 100-104 0.0 1101 105-109 0.0 1101 110-114 0.0 1101 115-119 0.0 1101 120-124 0.0 1101 125-129 0.0 1101 130-134 0.0 1101 135-139 0.0 1101 140-144 0.0 1101 145-149 0.0 1101 150-151 0.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 18 3.0 19 2.0 20 1.0 21 2.0 22 5.0 23 5.0 24 6.0 25 5.0 26 11.0 27 12.0 28 23.0 29 26.0 30 24.0 31 41.0 32 48.0 33 78.0 34 155.0 35 440.0 36 2926.0 37 187.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 46.82182182182182 13.98898898898899 6.531531531531531 32.65765765765766 2 19.825 13.825000000000001 34.1 32.25 3 17.525 19.075 30.599999999999998 32.800000000000004 4 22.15 24.275 25.424999999999997 28.15 5 23.549999999999997 32.35 23.724999999999998 20.375 6 19.325 33.75 24.675 22.25 7 16.025 28.749999999999996 38.725 16.5 8 18.175 28.7 30.55 22.575 9 15.75 24.0 36.8 23.45 10-14 19.500975048752437 28.88644432221611 28.021401070053503 23.59117955897795 15-19 19.865 27.779999999999998 27.810000000000002 24.545 20-24 20.45 28.12 27.800000000000004 23.630000000000003 25-29 19.675 28.43 28.535 23.36 30-34 19.939999999999998 28.685 27.134999999999998 24.240000000000002 35-39 19.375 27.985 27.905 24.735 40-44 20.52 28.67 27.200000000000003 23.61 45-49 20.31 27.345000000000002 28.16 24.185000000000002 50-54 20.69 28.860000000000003 26.8 23.65 55-59 20.375 27.66 27.55 24.415 60-64 20.04 27.450000000000003 27.455000000000002 25.055 65-69 20.135 27.950000000000003 27.474999999999998 24.44 70-74 20.395 27.005000000000003 29.04 23.56 75-79 19.869999999999997 27.61 28.125 24.395 80-84 20.27 27.215 27.750000000000004 24.765 85-89 20.78 27.63 27.63 23.96 90-94 21.044999999999998 28.08 27.79 23.085 95-99 20.62 27.43 27.66 24.29 100-104 20.905 28.395 27.495000000000005 23.205000000000002 105-109 20.97 27.825 27.589999999999996 23.615 110-114 21.905 27.93 26.745 23.419999999999998 115-119 21.34 28.22 26.705000000000002 23.735 120-124 21.44 27.96 26.375 24.224999999999998 125-129 21.21 27.63 26.515 24.645 130-134 21.04 28.425 26.045 24.490000000000002 135-139 20.775 27.72 26.419999999999998 25.085 140-144 22.28 27.62 26.655 23.445 145-149 20.9 28.799999999999997 26.215 24.085 150-151 22.3 26.6625 25.474999999999998 25.5625 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.5 4 0.5 5 0.5 6 1.0 7 0.5 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 1.0 17 1.0 18 0.5 19 0.5 20 2.0 21 3.5 22 1.5 23 0.0 24 3.0 25 8.0 26 8.5 27 6.0 28 8.0 29 15.0 30 16.0 31 14.0 32 19.0 33 35.0 34 51.0 35 57.5 36 74.0 37 104.0 38 124.0 39 149.0 40 167.5 41 183.5 42 226.0 43 237.5 44 236.0 45 303.0 46 322.5 47 268.5 48 234.0 49 221.5 50 195.0 51 142.0 52 110.0 53 90.5 54 79.5 55 70.0 56 52.5 57 37.0 58 28.0 59 23.0 60 14.5 61 10.5 62 12.5 63 8.5 64 4.0 65 1.5 66 0.0 67 1.5 68 3.0 69 2.0 70 1.5 71 3.0 72 3.0 73 1.0 74 0.5 75 0.5 76 0.0 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.1 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.005 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150-151 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 58.675 #Duplication Level Percentage of deduplicated Percentage of total 1 61.09927567106945 35.85 2 22.709842351938644 26.650000000000002 3 8.691947166595654 15.299999999999999 4 3.6642522368981676 8.6 5 1.9173412867490414 5.625 6 1.0651896037494673 3.75 7 0.3834682573498083 1.575 8 0.21303792074989347 1.0 9 0.08521516829995739 0.44999999999999996 >10 0.17043033659991477 1.2 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CCCAGCAACTTAAGTACATGACCTGCCTCAGTGACTCCTCGGATTGCCTT 14 0.35000000000000003 No Hit GCTTGAGATCCTTGACAGCAACATTTAACATTGAAACCAACATTGTCACC 12 0.3 No Hit GTTCCCAGCAAACCACTTCCAGTAGCCATCTCTGTCTTTCTTAAGTTGAA 12 0.3 No Hit CTTAATGGCACTTACCCTATCTATGATGCAAAAATACACAGAGATTGACA 10 0.25 No Hit CCTGGCTCAATCTCTACCATGTGGGGTTGCACTCCTGCCATTTTCATCAG 9 0.22499999999999998 No Hit CTGTGATTGAAGGGTAATTGAGGTTCTCTGGCAATGGTTTCTTCTCTAGA 9 0.22499999999999998 No Hit GTTCGTCTCTTGCGGTCCAAACTCCCCAAACCCAAACCAAGTGGAACAGC 8 0.2 No Hit CAGCTTTTTCTTGTGCTTTCCTTCTAGAAACTTCGTTATCGCATACTGGC 8 0.2 No Hit CTCAAATTCTCCACTGCCAACAACATAAAAGCAATCACCTTCACCACCCT 8 0.2 No Hit CCCGCATCCGACGCCACTGGCACGTGGTCTCTTGGGTTGAGGCTTGGCAG 8 0.2 No Hit GCACGGTTGACCTAGCTAATTTAGGTGAAATAAGCAATAACAGAGATTTT 8 0.2 No Hit GTTCATTATATTTCTTTGTGGGGATCTCCCGTACCCATGATCTAACTATT 7 0.17500000000000002 No Hit GTAGGATTGTCACCAATCAGGCAATCACCACCACTGATGAACTAGAACTC 7 0.17500000000000002 No Hit CAGAAAGAAACCAAATAGGAATTGGCACCTTCTTACCGTGATGTTTGAAA 7 0.17500000000000002 No Hit GGTGGGCTACGTACTGTGAAATTGCTCTTGATTCAAAGAGCTTAAGATCT 7 0.17500000000000002 No Hit CCCGAGCCCAGCCCTCAGAGCCAATCCTTTTCCCGAGGTTACGGATCCAT 7 0.17500000000000002 No Hit GCTCATTTTAGCTAAAGCCCTGCTTGCATCCTTGCCTGCAAACATTGCAT 7 0.17500000000000002 No Hit CCAAAAACCCTAGACCGATAATCCTTGAGTCTCGTAACAGGACACTCGTA 7 0.17500000000000002 No Hit GACCAAAGTACAAAAACGTTGGATCGGTGGCACCTTCTTTGTAGTAAGCA 7 0.17500000000000002 No Hit GGTCAAATTCGTTGTGGCTATGAGTGCGAGTGTTGGTAGATCCTGCACTT 7 0.17500000000000002 No Hit CACCTTAAGAGCATCAGGGATAACCATCCTCTTCTTCTTGTCATAAGGAG 6 0.15 No Hit CCGGCGACTGAATGGGCTCAACTTTGCAAGTACCCTGCATCTGCAAATTC 6 0.15 No Hit GCTAGAGAGAAATGCCATATGAAACAGGATGACACATTACAAACTTGACC 6 0.15 No Hit AGCAAACTTGAAAATTCTCAAACACAGAACCCATCAATTTTAGAGGATCC 6 0.15 No Hit CTTCTGCGAAGCCATTGCCATTACCCCTTTCATAATCCCCCATGTCCTAT 6 0.15 No Hit CATCAGTGAAATTGTGGATTTTGCAAACAATAGCTAAAGCAAGCTCGCAA 6 0.15 No Hit GCCAGCTTCAAGAGGGAAATTCAAGATCGCTTTGCTTCCTCGTAACCTGA 6 0.15 No Hit CCAGCATCTCCTTTCTACTGCTCATAACCGTATTGAACACTTCATTCACG 6 0.15 No Hit CTCCATTATGGTGTCTCCATATTGAGCAACAACTGCCTTGCACTCTTGAC 6 0.15 No Hit AGGCAGCTTCATCTTCCACAATGCCTTGCAATACTGCACTGGAATTCATG 6 0.15 No Hit GGCACCCTTTCCCCAGGTATAAACCTCATTTCTGGATGTCAAAACTGCTA 6 0.15 No Hit CTATGAGGTATGTACTTGAGGCAGCCCCAAACCACTTGGCCTCCCATGGA 6 0.15 No Hit GGATAGAAAAAATCAGTCAGTCTCATACAAGTAAGAAATTAAAATTGCAC 6 0.15 No Hit CTCTTGTTCTAGAATCTTAGCAGCCTGAGCCTTGTCCAAAGTTGGAAGAG 6 0.15 No Hit ATGAAAATAGACCAGCCTCCAGCAATAAGACACACAACAGCAGCTGCATG 6 0.15 No Hit AGGGATAAGTATACTTCACAATAGACCATTGCCATGTTCGGAAGGATATT 6 0.15 No Hit GCCTTGAATATCATATTCCTGAGCCAACTCATTCTCTTCCGTTGCATCCA 6 0.15 No Hit GCATAGTTCTCCAAAGAGTTCTTGGACTCGACCTTCTTCTTGTGCTCCTC 6 0.15 No Hit GCATTTATAAACTGTACTAATTGAATCCTGAGAGGACATCATTATGACTG 6 0.15 No Hit GCTTCAAACTGGTATCTATATACAGAAGTTACAATGAGAGAAAATGCAAA 6 0.15 No Hit GGTAGAATTACTGGCACTCCAATGATTCCATATAACGGCCATAATGGAGC 6 0.15 No Hit TTTTTTAACCGAAGCAGTGGGCTTTTGTTCATCCTCCACAGGTTCAGCGT 6 0.15 No Hit GTCAGGCATGTGGCTCCATCTGATTGAGATGGTAGTTGCCCTCATTGTTC 6 0.15 No Hit GCGACCTGTTGAGGTGCTCTGTGGCTCTCTTGAGCATGTCTGTGTAATAG 6 0.15 No Hit AGGGACAGCAATTTGTTTAAAAAACAGAAGGGGGCCAGAAGGGGCGGAAA 6 0.15 No Hit GGCCATCATAAAAGAGAATTCTGCCGTCGGCAACACCAGTGTAAGGACCA 5 0.125 No Hit TAGCATAACTGCTCAGAATACAGAATATAAAGAACGCAATACCGGGAGAA 5 0.125 No Hit AGGAGGGAGAAGTGACAATGATAGGGACAACTGGATTAGCAATAGCGGAA 5 0.125 No Hit GCCTTCAGTGACTGTGAGATGCCGTCTACGAGTGGCACGAATCGCACGGA 5 0.125 No Hit GCTTTTATTACCCCAGCTAGATTCCTGGTTTCCATCTCTCTTGCTGTCCT 5 0.125 No Hit GCCGTAGAAACTTGTTTGTTTCTTCTTCGAGAAACTTTCTGCAGCAGTTG 5 0.125 No Hit CCCATTACCTATGTTCTCCAGACGACCAAAGCTATGCGGACATGATGACG 5 0.125 No Hit GGTTCATATTCTCCACTTTTCTTCACCTCACGATTCCCAAGGCAGAAAAA 5 0.125 No Hit TTTGATTGTTGTACCCAAAACTATAACAGAAAAGATTAGGGTGCAGATCA 5 0.125 No Hit GCTCCATCACATGCACTACAACTAAAAAGGAAACTTCCACCTAAATACTT 5 0.125 No Hit CTCCATTATTAGGATCTTCAAGAAATTTAGCTGCTCCCTCTACCCCAACC 5 0.125 No Hit GCAAAGGTGATGATCCAAGGGCCAACGGACTCAAAGTTGTCATGTCCACT 5 0.125 No Hit CAACCTCAAATCCTCCTCAACAAACTCAATCTGCTCTTTAGTGCTCCTAA 5 0.125 No Hit CTTGATTTTGCGAGTTCTAAGCCACAGCAGGGATGCTGGAATTTTGGCGA 5 0.125 No Hit GTTGAGTTGGGACTTCCCATCACATATGCCACACTCTCTAAACCAAATTT 5 0.125 No Hit GTATATTATAATCCTTTAGAGTCCTCATTATGTCATAAAGAAGACCTTTG 5 0.125 No Hit CTCATAAATCAACAGGTTGCAATCCGAACTCGAGAAATAACAGTACAACT 5 0.125 No Hit GCAGCTTTAGCAACAACTTCACCTTCAGTGAAGATATAGCTAATGGAGTC 5 0.125 No Hit GCTCATGTCAGGGTACATCTTGCATCCTCCACAGCCGCTGCCGCACTTGC 5 0.125 No Hit CCAAGAACTTCTTCTGGCAGGGTTTCAGGTACCTGCCATAGCTTAATTTC 5 0.125 No Hit CAGAGATCGATTCCTGCGACGGTGATCAACTGCAATTCCAATTGTTGGAG 5 0.125 No Hit CTTCATGCCAACATCATAAATCCTGATCTTAGAGTCAGGCACACCACGGC 5 0.125 No Hit CTTGCATCAACCAAAATAAGACTCCATAATTTTATTCATAATTGCCCATT 5 0.125 No Hit CATGACTGCAAATGGTACCACATCACGGTACTCGTAGCACAAGGGTCTAT 5 0.125 No Hit GCCAAGACTTCTCAGCTATATCCTACTTGCTGCTGCTGGCCCTGCTGATA 5 0.125 No Hit AATTTCAAAGTGAGAAGGCAGTTAATATCATAAGAATAGAAGCAAACCTG 5 0.125 No Hit GCTTCCGCGGGATTGTGCTTCATTCACGGTGATGTTACGCCCATCAAGGT 5 0.125 No Hit GTCAGCTTTCTTATGTCCTGGCAATTTCTCCTTGATTTTGTCAAGGAAAC 5 0.125 No Hit GCATTGCAGTAGGGGTCGGAATGGTGTTTGGTAGACAAAAGGAAACTCTG 5 0.125 No Hit TGTTAAACCAACTCTTTTCTTGCAAGAGGTGCACCGGCTTGGCCCCTCCT 5 0.125 No Hit AGATGCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTT 5 0.125 No Hit GGTTTCTCATCTTTCTCATCAGACACGTCAGCATCAAAATTTGTGCTTTT 5 0.125 No Hit CCCTGTCTTTCCTGATTGAACTATCATTTCTATGCAGTGTGTTGTCCAGG 5 0.125 No Hit CCAGCATATCACAAATCCTGTGTCCCTCAAGAAAGCTGGCCGCAATCAGC 5 0.125 No Hit TGAAACAATCGAGCAATAAAAAGCAACAAGAAACACAGAAAAACATAGTA 5 0.125 No Hit GAGTTTTTCATCATTGATGCTATCATCTAGATTCTTGATATACAAATTCA 5 0.125 No Hit GCTGCGTAACTTGCTTCATTGCTTTGCTTCCAGAGGAACCAGCGAGAGCA 5 0.125 No Hit ACTCTTCCTCTTACGGCGTTGCCGATCATCCTCTTCATCTGATGACTCGT 5 0.125 No Hit ATCACGACTTCTATCAGGACTGTATCCATCTTTCCTTTCATCATCATCTC 5 0.125 No Hit GGCTAAAGAAACGACCATGATTGAGATCCCAATTATTATCAATGGCCACT 5 0.125 No Hit AGGCAGTAAAGCTCCCAGCAAGCATTGCCGACCTGAATACCGGCTTGACC 5 0.125 No Hit CTCTGCTTCCACTTCATCAAAGTCATCAGCCCTCTTCTCCAAACTCCTTT 5 0.125 No Hit GCATGTTTTGCTGCTATGATCCTTGAATATTTTTATCAATTTAAGCCCTT 5 0.125 No Hit GTGTATATGTAGGAAGGCTAGAGGTACAGATTGGTATCCGGATTCCTGGA 5 0.125 No Hit CAAAGAAAATCACCAAAGCCAAGCTATTTACACACGGGGAAGGGGGGAAA 5 0.125 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0 0.0 0.0 0.0 0.0 64-65 0.0 0.0 0.0 0.0 0.0 66-67 0.0 0.0 0.0 0.0 0.0 68-69 0.0 0.0 0.0 0.0 0.0 70-71 0.0 0.0 0.0 0.0 0.0 72-73 0.0 0.0 0.0 0.0 0.0 74-75 0.0 0.0 0.0 0.0 0.0 76-77 0.0 0.0 0.0 0.0 0.0 78-79 0.0 0.0 0.0 0.0 0.0 80-81 0.0375 0.0 0.0 0.0 0.0 82-83 0.15 0.0 0.0 0.0 0.0 84-85 0.175 0.0 0.0 0.0 0.0 86-87 0.2 0.0 0.0 0.0 0.0 88-89 0.2 0.0 0.0 0.0 0.0 90-91 0.2625 0.0 0.0 0.0 0.0 92-93 0.35 0.0 0.0 0.0 0.0 94-95 0.475 0.0 0.0 0.0 0.0 96-97 0.5625 0.0 0.0 0.0 0.0 98-99 0.7625 0.0 0.0 0.0 0.0 100-101 1.1 0.0 0.0 0.0 0.0 102-103 1.4249999999999998 0.0 0.0 0.0 0.0 104-105 1.7875 0.0 0.0 0.0 0.0 106-107 2.0375 0.0 0.0 0.0 0.0 108-109 2.4375 0.0 0.0 0.0 0.0 110-111 2.7750000000000004 0.0 0.0 0.0 0.0 112-113 3.25 0.0 0.0 0.0 0.0 114-115 3.65 0.0 0.0 0.0 0.0 116-117 4.2625 0.0 0.0 0.0 0.0 118-119 4.9 0.0 0.0 0.0 0.0 120-121 5.4125 0.0 0.0 0.0 0.0 122-123 6.1125 0.0 0.0 0.0 0.0 124-125 6.7 0.0 0.0 0.0 0.0 126-127 7.1375 0.0 0.0 0.0 0.0 128-129 7.7625 0.0 0.0 0.0 0.0 130-131 8.3125 0.0 0.0 0.0 0.0 132-133 9.15 0.0 0.0 0.0 0.0 134-135 10.3875 0.0 0.0 0.0 0.0 136-137 11.0625 0.0 0.0 0.0 0.0 138-139 11.85 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AAGATCC 10 0.006830828 145.0 4 >>END_MODULE SRR26075393 read2 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR26075393_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.2075 37.0 37.0 37.0 37.0 37.0 2 36.279 37.0 37.0 37.0 37.0 37.0 3 36.2315 37.0 37.0 37.0 37.0 37.0 4 36.4035 37.0 37.0 37.0 37.0 37.0 5 36.3255 37.0 37.0 37.0 37.0 37.0 6 36.225 37.0 37.0 37.0 37.0 37.0 7 36.2585 37.0 37.0 37.0 37.0 37.0 8 36.288 37.0 37.0 37.0 37.0 37.0 9 36.308 37.0 37.0 37.0 37.0 37.0 10-14 36.2784 37.0 37.0 37.0 37.0 37.0 15-19 36.194599999999994 37.0 37.0 37.0 37.0 37.0 20-24 36.1029 37.0 37.0 37.0 37.0 37.0 25-29 36.0175 37.0 37.0 37.0 37.0 37.0 30-34 35.859300000000005 37.0 37.0 37.0 37.0 37.0 35-39 35.853899999999996 37.0 37.0 37.0 37.0 37.0 40-44 35.7324 37.0 37.0 37.0 37.0 37.0 45-49 35.7385 37.0 37.0 37.0 37.0 37.0 50-54 35.587799999999994 37.0 37.0 37.0 37.0 37.0 55-59 35.6441 37.0 37.0 37.0 37.0 37.0 60-64 35.6514 37.0 37.0 37.0 37.0 37.0 65-69 35.5901 37.0 37.0 37.0 37.0 37.0 70-74 35.5167 37.0 37.0 37.0 37.0 37.0 75-79 35.432399999999994 37.0 37.0 37.0 37.0 37.0 80-84 35.4485 37.0 37.0 37.0 37.0 37.0 85-89 35.4673 37.0 37.0 37.0 37.0 37.0 90-94 35.396100000000004 37.0 37.0 37.0 37.0 37.0 95-99 35.41759999999999 37.0 37.0 37.0 37.0 37.0 100-104 35.2825 37.0 37.0 37.0 37.0 37.0 105-109 35.3634 37.0 37.0 37.0 34.6 37.0 110-114 35.1555 37.0 37.0 37.0 29.8 37.0 115-119 35.1977 37.0 37.0 37.0 32.2 37.0 120-124 35.138400000000004 37.0 37.0 37.0 29.8 37.0 125-129 35.0606 37.0 37.0 37.0 27.4 37.0 130-134 35.02910000000001 37.0 37.0 37.0 25.0 37.0 135-139 34.7832 37.0 37.0 37.0 25.0 37.0 140-144 34.885400000000004 37.0 37.0 37.0 25.0 37.0 145-149 34.887600000000006 37.0 37.0 37.0 25.0 37.0 150-151 34.576499999999996 37.0 37.0 37.0 25.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.0 1101 2 0.0 1101 3 0.0 1101 4 0.0 1101 5 0.0 1101 6 0.0 1101 7 0.0 1101 8 0.0 1101 9 0.0 1101 10-14 0.0 1101 15-19 0.0 1101 20-24 0.0 1101 25-29 0.0 1101 30-34 0.0 1101 35-39 0.0 1101 40-44 0.0 1101 45-49 0.0 1101 50-54 0.0 1101 55-59 0.0 1101 60-64 0.0 1101 65-69 0.0 1101 70-74 0.0 1101 75-79 0.0 1101 80-84 0.0 1101 85-89 0.0 1101 90-94 0.0 1101 95-99 0.0 1101 100-104 0.0 1101 105-109 0.0 1101 110-114 0.0 1101 115-119 0.0 1101 120-124 0.0 1101 125-129 0.0 1101 130-134 0.0 1101 135-139 0.0 1101 140-144 0.0 1101 145-149 0.0 1101 150-151 0.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 13 3.0 14 15.0 15 12.0 16 10.0 17 8.0 18 5.0 19 1.0 20 5.0 21 7.0 22 8.0 23 5.0 24 7.0 25 12.0 26 9.0 27 10.0 28 16.0 29 34.0 30 19.0 31 42.0 32 56.0 33 94.0 34 202.0 35 712.0 36 2523.0 37 185.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.0 24.099999999999998 9.375 21.525 2 28.825 27.1 26.55 17.525 3 22.8 26.85 31.924999999999997 18.425 4 25.45 32.675 24.025 17.849999999999998 5 27.200000000000003 33.475 23.025000000000002 16.3 6 21.925 38.85 21.875 17.349999999999998 7 22.75 21.375 35.949999999999996 19.925 8 22.35 26.875 25.674999999999997 25.1 9 23.849999999999998 26.325 27.675 22.15 10-14 25.16 28.71 24.925 21.205 15-19 25.635 27.87 26.490000000000002 20.005 20-24 23.755000000000003 28.37 26.25 21.625 25-29 25.245 28.560000000000002 25.05 21.145 30-34 24.775 28.285 26.27 20.669999999999998 35-39 25.45 27.169999999999998 26.61 20.77 40-44 24.990000000000002 28.595 25.919999999999998 20.495 45-49 25.06 27.700000000000003 27.32 19.919999999999998 50-54 23.68 27.57 28.115000000000002 20.635 55-59 25.305 27.66 26.76 20.275000000000002 60-64 24.48 28.27 26.855 20.395 65-69 25.380000000000003 28.299999999999997 25.805 20.515 70-74 24.795 28.985 26.33 19.89 75-79 25.09 28.685 26.27 19.955000000000002 80-84 24.625 28.67 26.474999999999998 20.23 85-89 24.65 26.83 27.860000000000003 20.66 90-94 24.875 28.49 26.605 20.03 95-99 23.915 28.785 26.474999999999998 20.825 100-104 23.965 28.435 26.39 21.21 105-109 24.915000000000003 27.575 27.355 20.155 110-114 24.555 28.884999999999998 26.355 20.205000000000002 115-119 24.27 28.325 26.435 20.97 120-124 24.665 28.74 27.235 19.36 125-129 24.990000000000002 28.83 26.325 19.855 130-134 25.105 28.615000000000002 26.375 19.905 135-139 26.56 28.615000000000002 25.430000000000003 19.395 140-144 26.375 28.865000000000002 25.465 19.295 145-149 26.685 28.27 25.775 19.27 150-151 27.275 26.637499999999996 26.525 19.5625 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 1.0 7 1.0 8 0.0 9 0.5 10 0.5 11 0.0 12 0.5 13 0.5 14 0.0 15 0.5 16 1.0 17 2.0 18 2.5 19 1.5 20 0.5 21 0.0 22 1.0 23 1.5 24 0.5 25 1.0 26 1.5 27 0.5 28 4.0 29 4.0 30 7.5 31 13.0 32 20.0 33 25.5 34 32.5 35 55.5 36 58.0 37 79.5 38 106.5 39 130.0 40 183.0 41 221.5 42 271.0 43 294.0 44 262.5 45 247.5 46 261.0 47 254.0 48 236.5 49 217.5 50 194.0 51 163.0 52 129.0 53 102.5 54 86.0 55 69.5 56 49.5 57 42.0 58 26.5 59 19.5 60 19.5 61 17.0 62 9.5 63 4.5 64 4.0 65 2.5 66 3.5 67 2.0 68 2.0 69 1.5 70 0.5 71 2.5 72 2.5 73 2.5 74 4.0 75 2.0 76 0.5 77 1.0 78 0.5 79 0.5 80 2.5 81 4.0 82 2.5 83 1.5 84 2.0 85 2.0 86 1.5 87 1.5 88 2.5 89 2.5 90 1.5 91 1.5 92 1.5 93 0.5 94 0.0 95 0.5 96 0.5 97 0.0 98 0.5 99 0.5 100 2.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150-151 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 59.724999999999994 #Duplication Level Percentage of deduplicated Percentage of total 1 63.87609878610297 38.15 2 20.468815403934702 24.45 3 8.455420678107995 15.15 4 3.3486814566764336 8.0 5 1.8836333193804937 5.625 6 1.1301799916282964 4.05 7 0.2930096274591879 1.225 8 0.25115110925073253 1.2 9 0.041858518208455424 0.22499999999999998 >10 0.25115110925073253 1.925 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG 16 0.4 No Hit AGGCTTTGAAAGATCAAGGATTTTGCAGATGGAACTACACAATAAGATCA 14 0.35000000000000003 No Hit AAAAGAACGGTGGTCATCCCCTTGAGAGCACCAAGTGCTACCAGGTACCT 13 0.325 No Hit AATGGTTGTGACCTTTGGCCCCTCTGGGCTGACCACTGAAGTTATATCTG 12 0.3 No Hit GATGCTTAAAACTGGTAGGCTCTCTGAACCCTACAAGGGCATTGGTGATT 11 0.27499999999999997 No Hit GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGT 11 0.27499999999999997 No Hit GAAAGAAAGCAAGCACGAGCACAGAAAGATGGTGAACCTAGTGGCAGCAC 9 0.22499999999999998 No Hit CCAGCCCATAGGAAAACTTGGGAAGAAGAAGCACACATTGAGAGAGCATT 8 0.2 No Hit TAGAGATGGAGATGTTGCTCCTCTTCATCCTCCTCCTAAGTGGAAGGATT 8 0.2 No Hit TCTCAATCTTGATCGAGCAATGGATCCAGGGTTGGTTTACGATATTACCA 8 0.2 No Hit TGAGGTCAACGAACGAGAGCTGGCAGCCCTCAAGGCTGTGATCAAGTGCA 8 0.2 No Hit GTCTTCTTCAGTGAACATACCATCATCTTCAGTGAACATATCATCTTCTT 8 0.2 No Hit ACCAAAGCCAAGAAACCCAAATCCCCTCGCGCTTATCCATCTTTCCACGT 8 0.2 No Hit GGGAGCCACCAAGTTCTTGCTTTCGAAGATCAAGGACTTCCAATTCTTTG 7 0.17500000000000002 No Hit CGGCGGCCCTTGAAAATCCGGAGGACCGAGTGCCTCCCACGCCCGGTCGT 7 0.17500000000000002 No Hit GAAATCTTTCTTGAATGTCATTATAGTAGGTGGCAAAGATGTTGTCCTGG 7 0.17500000000000002 No Hit TAGGAATGGGAGTATTTGCACTTGTGGTAACGGTATTTGCATTATTGATG 7 0.17500000000000002 No Hit AAAACAAAATTTCACTTCAGGTATAAAGTAGGCTTAGTGGAAGGAGAGCA 7 0.17500000000000002 No Hit ATGGGAATGACACCGCTCTAATCAGAAGAGCTTTACTGTCTTGTTTATAT 7 0.17500000000000002 No Hit CAAAAACCGTATCTAACGGAAGCGGTAAGACTCACGAGTGCTCCATCTGC 7 0.17500000000000002 No Hit GGTTTAGAGGCTCATTCTACATAGGAACAGTGGTGAAGGCAACAAGAACC 6 0.15 No Hit TGATGAAAAAGAAGGGGGTGAAATTGTTTTTGGTGGGGTAGATCCTGATC 6 0.15 No Hit CTTCTGAGTTGGCCGAGTGTCGTATCAGGCGCCCTTTTTTAATTGTCGTC 6 0.15 No Hit CTTGCTCTCACGATGCTTAACAGTTGCATTTTCTCGGCTGAGATTAGGAG 6 0.15 No Hit GATATACTCTATCAATGGCTGTAAAAATCTATATCATTTACTACTCTATG 6 0.15 No Hit AGTACTGTTTATGGTCAACTTGGAAATCCTTATGCTGATGGAAAGGTACC 6 0.15 No Hit GGCTAGAATAGATATGATTGCAGCAGCCTGTGAAGGTGCTGAAAAAGTAT 6 0.15 No Hit AGGAACTCCTCAGTGGAGAGAAAGAAGGTCTTATCCAGCTCCCTACAGAT 6 0.15 No Hit AAATTATGTTGGGTGGTGTCCTTTTTGGTGAAGGGAAGCAACCCTGTTGT 6 0.15 No Hit AGATGATTCTGCAAAATTTGATATGCTTCTCAAGGTGAAGAGGGGAAGCA 6 0.15 No Hit GATTAAGAAGAGGGTAAGTAGCGATTCAGAGGATGGGGATGAGGATAGGA 6 0.15 No Hit GGCATGGGAGATACTGAAAGGAAGGCCACATGGCATAGACCTCATATTGA 6 0.15 No Hit GATGAAGCAGGCGGAGAAAAAGGGGAGAGCCTAAACGGTGCCGTTAAGGA 6 0.15 No Hit AGACGATGATGTTCAAGATCAACTAGATGAGGATGGAGAAGATGATGCTG 6 0.15 No Hit GCTTGCTGATCACTTAGAACTAGGATCCAAGTTTTATGTGATGGGGTTCT 6 0.15 No Hit ATGTTGGGAAAGCCCAGAAAAAGTCTGAAAGAGAGCTTGAACTGAAAGGG 6 0.15 No Hit CAGCTCTTCATCAAACTCCAATAAACAAACAATTCAAACACATCAACAAG 6 0.15 No Hit GAGGGACAACAACTTGCTTGGCAAATTTGAGCTCTCAGGCATTCCTCCTG 6 0.15 No Hit CAGAGCTAGAGAGAATCTAAGATTTTGCAGTCTCAAATTCAGAAGGGAGA 6 0.15 No Hit CCGCTAAGCACTAAGCACCTCACCACCGAATCCCACTTTCGACCTACGAT 6 0.15 No Hit AGATTGACGACAAGGACGTCGTCGTTTTGAAGGAAGGGAATTTCAGTGAT 6 0.15 No Hit ATTTTGTATATCAGTAACTTCTAGTTCTGTATGACTGCTACACCTTAGTG 6 0.15 No Hit CCTTGAAACTCCATGGAACCCCTATATCGACCAACACACAGCGAGTTCTT 6 0.15 No Hit CCTACTACTACTACACCTCTCTCCGCTTTTGCTTCACCTATTCACCGGAG 6 0.15 No Hit CATCAATCGGATCTCATTCCAGGCTCAACAAGACATCTCTTCTCTGAAGA 6 0.15 No Hit GAGATCCGTGACCCTAACAAAAAAGGAGCTCGTGTATGGCTTGGAACATT 6 0.15 No Hit CACAAGACTAAGAAAGGGGAGGCAGCACTTGCAAGATTGAAGGCTTATGA 6 0.15 No Hit GAGAGAGACGGAGCAGAGAGAGAGATCCGACGCGCTTGTTGCAGAAAGGG 5 0.125 No Hit GGGAAACATGTGGTGTTCGGAAAAGTTGTGGAGGGTATGAATGTTGTCAA 5 0.125 No Hit TTCTATCTAGGGTTTGGGCTTGATAATAATTTCTTTGAAGATGTTGGAGG 5 0.125 No Hit GGAGCACCCAAAACATGATGACAATCATCATGACCACTCGAACAATGAGC 5 0.125 No Hit GCGATAACATCGTCGGTCCCACCGGATGGCAACTCGCCGAGATCGACACA 5 0.125 No Hit CACAAGAGCAGATTTCAACAAAGTGCTTGCACGGCAGAAGCCAACAGTGA 5 0.125 No Hit CGAAAAATTCGTGAATCTTCAACATCTATCTATAGCGAACATTGGTGTTT 5 0.125 No Hit AGTCTATCCACATCTTAGGACAGCGGTGTGATGATCTTGGAGGAGATCCA 5 0.125 No Hit GTTTTATAGTTTAGTTAGACGGTAAAAGCGGCTAACAGGTTTTCTTTTAT 5 0.125 No Hit GGGCCATGGAGGATCTGGGACTGTGTACAGAATTGAACTGAGCAGTGGAG 5 0.125 No Hit CTTTGTTCTTGTCTACACTGTCTTCTCTGCTACTGATGCCAAGAGAAACG 5 0.125 No Hit GGTTTCTGCGCCGATCTACTGCACACAAAGTCGCCATGGGTAGAAGACCT 5 0.125 No Hit CTCTCATTGGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCA 5 0.125 No Hit AAGCACTGGACGCCACAAACTTAAGCAAGCTGCTGGATCGAGTGATTTCA 5 0.125 No Hit GGCCACTACTGACCAATCCCTTCAAGAAGCGTTTAGCCAGTACGGTGAAA 5 0.125 No Hit GCCACAGGCGTCATACTTCCCAAGAAGCGGCCATAGCCCAGATGCGAGGT 5 0.125 No Hit CTCGAGCTGTAATTGGCTCTTTAGGTAGATCGAATAAGATAGTTGCCGAG 5 0.125 No Hit AGAGTGTCGAGTATTGGCAACAGGACAAGTGGACAGGCTATTTCCCTGTG 5 0.125 No Hit TTAGAGAGGAAGGGAGAGGGAGAGAGAGAGAGGAGGGGGAGCTCAAGATC 5 0.125 No Hit CCCATTTTTGCCAGTTTATATTGCCTTTTCTTTGCTTACGTGGGTGCTGC 5 0.125 No Hit GGAGTGGCAAAGTTCAATGTCCCCCTGGCCCATATGATCATTGCTGGTGC 5 0.125 No Hit GAAGTCTTCTGCCCATTTGGTGATGAGTTCTCAGCACCACAGTTCACAGA 5 0.125 No Hit GGGCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATAGGAGGTTT 5 0.125 No Hit TGATTGCTGGGTTGCTTAAAAATGCTGAGGTTGCATTGGATGCTCTGTCA 5 0.125 No Hit GTCGGAATCCAGTCTCTTTGTATACCCTTACAAAGTTATCATCAAAACTT 5 0.125 No Hit AATTTTATTAAATGGTGCTGTTAAAAAGGGTGAGCGCTTAGTACCACCTT 5 0.125 No Hit GATAAGCTAGGCAAGATGGTTTTACTAGACAAGATGTGGGATGATGTTGT 5 0.125 No Hit CTCTAGACAGCCACAGACTTGTAAATTTTGCCGGCCAACAGGGTCTTGAT 5 0.125 No Hit CTAACTCCCTCTCCAATCTCTCTCTCTCCCCCTCTCATGTAGAAGAAGAG 5 0.125 No Hit ATTTCATAGGCGTCTTGATAAACGCCACCCACCCTCAGCATCAATTCCAA 5 0.125 No Hit GCCCAAGGAAGAAGAGGAGTCTGAGAAGTCAATGTCAGAGGTCAATGATG 5 0.125 No Hit CATGGCCAGGTCCAACCTCACCATGCTGCTTCATGACTCACTATGACTAA 5 0.125 No Hit CATTCTCGAATCGCCTTTATCTGCATATATTCGCTCCCCCATGCTATCTA 5 0.125 No Hit CTACTCCCTAAAGAGAAGGATGAAGAAAACTTACTCCAAAGATCAGAAAT 5 0.125 No Hit ACCTACGGCCGGACCTCTTCGACCCATTGTACATGGCCAGACTTTGAAAT 5 0.125 No Hit GCTTGATTGTGAAGATTTGATGAGCTCTTGGATGAGTGGTTTGTGGTGTG 5 0.125 No Hit GAACTCCCTAGTTCACTAACTTCCACCAGTGTTTCTGTCAATATGGACAA 5 0.125 No Hit TATTCTTGCAAACTTAAACAGAGAGAGAGAGAGCCGAGAAAGAAAGATAG 5 0.125 No Hit GGGTCTCCTCCATCCAGAGTAGGCAACCCATTAATTCAAGATGCTAGATT 5 0.125 No Hit CCGTAATCCTTCAAATAAACAGGCTCAGCCAGATGCAAACCAGTATGGTG 5 0.125 No Hit AGGGATTATAATTCTGGGAGAAACATGGGGTTCAAACTGCCCTGTTGAAG 5 0.125 No Hit CTTAAGATAGTTAGCAAGATTGAGGCAGGGGAGAGGTTCACTGTTTATGT 5 0.125 No Hit GCTTTTATATCCCCTATTCTCTTCGTGAAGATGTCTTGCTGTGGAGGAAA 5 0.125 No Hit CCTAATGGTTGTACTGAAATGCGTTGAAGATTACAAGCTTGAAACTGTAT 5 0.125 No Hit TGAATGGATCGGCAAGCAGCAATGTGAATGAACCTGTTATTGCTGACACC 5 0.125 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0 0.0 0.0 0.0 0.0 64-65 0.0 0.0 0.0 0.0 0.0 66-67 0.0 0.0 0.0 0.0 0.0 68-69 0.0 0.0 0.0 0.0 0.0 70-71 0.0 0.0 0.0 0.0 0.0 72-73 0.0 0.0 0.0 0.0 0.0 74-75 0.0 0.0 0.0 0.0 0.0 76-77 0.0 0.0 0.0 0.0 0.0 78-79 0.0 0.0 0.0 0.0 0.0 80-81 0.0375 0.0 0.0 0.0 0.0 82-83 0.15 0.0 0.0 0.0 0.0 84-85 0.175 0.0 0.0 0.0 0.0 86-87 0.2 0.0 0.0 0.0 0.0 88-89 0.2 0.0 0.0 0.0 0.0 90-91 0.2875 0.0 0.0 0.0 0.0 92-93 0.375 0.0 0.0 0.0 0.0 94-95 0.5 0.0 0.0 0.0 0.0 96-97 0.6125 0.0 0.0 0.0 0.0 98-99 0.8125 0.0 0.0 0.0 0.0 100-101 1.15 0.0 0.0 0.0 0.0 102-103 1.4500000000000002 0.0 0.0 0.0 0.0 104-105 1.7999999999999998 0.0 0.0 0.0 0.0 106-107 2.0999999999999996 0.0 0.0 0.0 0.0 108-109 2.4875 0.0 0.0 0.0 0.0 110-111 2.825 0.0 0.0 0.0 0.0 112-113 3.325 0.0 0.0 0.0 0.0 114-115 3.7375 0.0 0.0 0.0 0.0 116-117 4.3375 0.0 0.0 0.0 0.0 118-119 4.95 0.0 0.0 0.0 0.0 120-121 5.6375 0.0 0.0 0.0 0.0 122-123 6.3875 0.0 0.0 0.0 0.0 124-125 6.9625 0.0 0.0 0.0 0.0 126-127 7.4 0.0 0.0 0.0 0.0 128-129 8.05 0.0 0.0 0.0 0.0 130-131 8.649999999999999 0.0 0.0 0.0 0.0 132-133 9.55 0.0 0.0 0.0 0.0 134-135 10.825 0.0 0.0 0.0 0.0 136-137 11.525 0.0 0.0 0.0 0.0 138-139 12.35 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content pass >>END_MODULE Read 779349 spots for SRR26075393.sra Written 779349 spots for SRR26075393.sra Read 779349 spots for SRR26075393.sra Written 779349 spots for SRR26075393.sra Read 779349 spots for SRR26075393.sra Written 779349 spots for SRR26075393.sra Read 779349 spots for SRR26075393.sra Written 779349 spots for SRR26075393.sra Read 779349 spots for SRR26075393.sra Written 779349 spots for SRR26075393.sra Read 779349 spots for SRR26075393.sra Written 779349 spots for SRR26075393.sra Read 779349 spots for SRR26075393.sra Written 779349 spots for SRR26075393.sra Read 779349 spots for SRR26075393.sra Written 779349 spots for SRR26075393.sra Read 779349 spots for SRR26075393.sra Written 779349 spots for SRR26075393.sra Read 779349 spots for SRR26075393.sra Written 779349 spots for SRR26075393.sra Read 779349 spots for SRR26075393.sra Written 779349 spots for SRR26075393.sra Read 779358 spots for SRR26075393.sra Written 779358 spots for SRR26075393.sra Read 779349 spots for SRR26075393.sra Written 779349 spots for SRR26075393.sra Read 779349 spots for SRR26075393.sra Written 779349 spots for SRR26075393.sra Read 779349 spots for SRR26075393.sra Written 779349 spots for SRR26075393.sra Read 779349 spots for SRR26075393.sra Written 779349 spots for SRR26075393.sra Read 779349 spots for SRR26075393.sra Written 779349 spots for SRR26075393.sra Read 779349 spots for SRR26075393.sra Written 779349 spots for SRR26075393.sra Read 779349 spots for SRR26075393.sra Written 779349 spots for SRR26075393.sra Read 779349 spots for SRR26075393.sra Written 779349 spots for SRR26075393.sra SRR ids: ['SRR26075393.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_cecugj1k SRR26075393.sra spots: 15586989 blocks: [[1, 779349], [779350, 1558698], [1558699, 2338047], [2338048, 3117396], [3117397, 3896745], [3896746, 4676094], [4676095, 5455443], [5455444, 6234792], [6234793, 7014141], [7014142, 7793490], [7793491, 8572839], [8572840, 9352188], [9352189, 10131537], [10131538, 10910886], [10910887, 11690235], [11690236, 12469584], [12469585, 13248933], [13248934, 14028282], [14028283, 14807631], [14807632, 15586989]] SRR26075393 file size 5750024 SRR26075393 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o SRR26075393 SRR26075393_1.fastq SRR26075393_2.fastq Input file: SRR26075393_1.fastq Paired file: SRR26075393_2.fastq trimmed: SRR26075393-trimmed-pair1.fastq, SRR26075393-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Wed Feb 12 00:25:41 2025 >> started Wed Feb 12 00:26:05 2025 >> done (24.353s) 15586989 read pairs processed; of these: 67 ( 0.00%) short read pairs filtered out after trimming by size control 36282 ( 0.23%) empty read pairs filtered out after trimming by size control 15550640 (99.77%) read pairs available; of these: 2521905 (16.22%) trimmed read pairs available after processing 13028735 (83.78%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 18 8 0.00% 19 9 0.00% 20 9 0.00% 21 8 0.00% 22 13 0.00% 23 8 0.00% 24 16 0.00% 25 14 0.00% 26 17 0.00% 27 17 0.00% 28 14 0.00% 29 24 0.00% 30 16 0.00% 31 16 0.00% 32 17 0.00% 33 22 0.00% 34 19 0.00% 35 25 0.00% 36 17 0.00% 37 15 0.00% 38 24 0.00% 39 32 0.00% 40 23 0.00% 41 38 0.00% 42 37 0.00% 43 41 0.00% 44 51 0.00% 45 40 0.00% 46 35 0.00% 47 58 0.00% 48 63 0.00% 49 80 0.00% 50 90 0.00% 51 86 0.00% 52 125 0.00% 53 101 0.00% 54 104 0.00% 55 119 0.00% 56 138 0.00% 57 131 0.00% 58 140 0.00% 59 171 0.00% 60 265 0.00% 61 309 0.00% 62 300 0.00% 63 349 0.00% 64 429 0.00% 65 432 0.00% 66 442 0.00% 67 559 0.00% 68 589 0.00% 69 684 0.00% 70 759 0.00% 71 951 0.01% 72 1087 0.01% 73 1247 0.01% 74 1455 0.01% 75 1595 0.01% 76 1759 0.01% 77 1992 0.01% 78 2161 0.01% 79 2486 0.02% 80 2847 0.02% 81 3170 0.02% 82 3818 0.02% 83 4114 0.03% 84 4437 0.03% 85 4924 0.03% 86 5566 0.04% 87 6078 0.04% 88 6499 0.04% 89 7002 0.05% 90 7789 0.05% 91 8548 0.05% 92 9645 0.06% 93 10508 0.07% 94 11561 0.07% 95 12417 0.08% 96 13383 0.09% 97 14210 0.09% 98 15031 0.10% 99 15946 0.10% 100 16664 0.11% 101 18208 0.12% 102 19142 0.12% 103 20292 0.13% 104 21595 0.14% 105 22747 0.15% 106 24851 0.16% 107 25205 0.16% 108 26687 0.17% 109 27685 0.18% 110 27591 0.18% 111 29309 0.19% 112 30485 0.20% 113 32135 0.21% 114 33763 0.22% 115 34799 0.22% 116 35947 0.23% 117 37744 0.24% 118 38340 0.25% 119 39153 0.25% 120 39979 0.26% 121 41398 0.27% 122 42390 0.27% 123 43474 0.28% 124 45186 0.29% 125 46079 0.30% 126 48741 0.31% 127 48756 0.31% 128 50032 0.32% 129 52181 0.34% 130 51744 0.33% 131 51614 0.33% 132 53945 0.35% 133 55078 0.35% 134 56038 0.36% 135 57200 0.37% 136 58758 0.38% 137 59283 0.38% 138 60420 0.39% 139 62266 0.40% 140 62900 0.40% 141 64250 0.41% 142 66192 0.43% 143 65636 0.42% 144 67008 0.43% 145 66493 0.43% 146 69357 0.45% 147 70054 0.45% 148 69399 0.45% 149 71384 0.46% 150 72954 0.47% 151 13028735 83.78% 15550640 reads passed initial QC criterion=sequence-density sequence-density=0.27 sequence-density-rank=1 fanout-score=3.32 fanout-score-rank=30 prefix-density=0.39 prefix-fanout=2.2 sequence=CACTTGCAGCCATTCTCAGCACCAGAG criterion=fanout-score sequence-density=0.05 sequence-density-rank=28 fanout-score=342.77 fanout-score-rank=1 prefix-density=0.78 prefix-fanout=22.7 sequence=TCATCATCACCACCATCACCATCCCTGATTGATCTTTGATTCACAACAAGACCAACCAACCGTACAATGTTTACATAACTTGGGATAATCTCACAAGAAAGAAAGATCAATACAAAACTAGTCAGCTCAGGATGTTCCAGGCACGGTAGAACCGTAGAACCATAACAACAAGAGACATATTGCAGATGAGTACTGAAAAACAAAACACAGTACGTATTTACATGGGCAACCTTGGTTGAAGGCAACCTCATCAACGATGCTCGCTCTTCGTCTCTCCACTGTACATCCAGTCATAGACAGTGGGTGTGTTAGGCTGTGGCTTGTCAAAAACATGAGCACCAATATTCTTAGTAGCAAGGTTGCTACCAGGGTGGAACACGCTCCTCCAAACATTGCTACGCGCCGACACTGGGGTTGTAGGGGTCACTGGTGTCGTCGGTGTCCCTGGAGTTCCTGGCATAGTCATGGACCTCTGAAACTTATTAACAGGACTGCTCCCCTCTCCGACGTC criterion=sequence-density sequence-density=0.31 sequence-density-rank=1 fanout-score=2.98 fanout-score-rank=29 prefix-density=0.32 prefix-fanout=2.9 sequence=ATGTACCCTGACTT criterion=fanout-score sequence-density=0.01 sequence-density-rank=38 fanout-score=325.40 fanout-score-rank=1 prefix-density=0.15 prefix-fanout=14.5 sequence=AGAGAAAAGATAAGCTAGGCAAGATGGTTTTACTA SRR26075393 testing PE reads STAR mapping to Ensembl genome Started job on | Feb 12 00:26:50 Started mapping on | Feb 12 00:26:51 Finished on | Feb 12 00:30:31 Mapping speed, Million of reads per hour | 254.47 Number of input reads | 15550640 Average input read length | 294 UNIQUE READS: Uniquely mapped reads number | 13597977 Uniquely mapped reads % | 87.44% Average mapped length | 292.79 Number of splices: Total | 12479698 Number of splices: Annotated (sjdb) | 12167865 Number of splices: GT/AG | 12239043 Number of splices: GC/AG | 189092 Number of splices: AT/AC | 16010 Number of splices: Non-canonical | 35553 Mismatch rate per base, % | 0.40% Deletion rate per base | 0.03% Deletion average length | 3.11 Insertion rate per base | 0.02% Insertion average length | 2.53 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 340514 % of reads mapped to multiple loci | 2.19% Number of reads mapped to too many loci | 58681 % of reads mapped to too many loci | 0.38% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 9.65% % of reads unmapped: other | 0.34% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 1612149 1612149 1612149 N_multimapping 340514 340514 340514 N_noFeature 335503 13445442 418137 N_ambiguous 142517 744 72262 UnstrandedReadsAssigned:13119957 PositiveStrandReadsAssigned:151791 NegativeStrandReadsAssigned:13107578 Dataset is classified negative stranded MeadianReadLen=151 20thPercentileLength=151 echo kmer=147 SRR26075393 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,400 [index] number of k-mers: 62,057,036 [index] number of equivalence classes: 130,681 [quant] running in paired-end mode [quant] will process pair 1: SRR26075393-trimmed-pair1.fastq SRR26075393-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 15,550,640 reads, 13,316,295 reads pseudoaligned [quant] estimated average fragment length: 210.557 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,259 rounds 52401 SRR26075393.ke.tsv 34699 SRR26075393.se.tsv 87100 total ==> SRR26075393.ke.tsv <== target_id length eff_length est_counts tpm Potri.005G200100.1.v4.1 2018 1808.44 1247 45.514 Potri.005G024800.1.v4.1 1035 825.443 1132 90.5196 Potri.004G059700.1.v4.1 961 751.443 1 0.087839 Potri.007G009000.2.v4.1 1416 1206.44 0 0 Potri.003G141000.2.v4.1 2943 2733.44 698 16.855 Potri.016G087400.1.v4.1 270 89.9096 1069 784.792 Potri.015G069301.1.v4.1 564 355.715 0 0 Potri.010G195200.1.v4.1 1773 1563.44 356 15.0297 Potri.012G127500.1.v4.1 977 767.443 12101 1040.78 ==> SRR26075393.se.tsv <== Potri.001G166300.v4.1 0 Potri.001G448400.v4.1 65 Potri.001G233950.v4.1 4 Potri.001G122700.v4.1 198 Potri.001G212900.v4.1 0 Potri.001G182400.v4.1 0 Potri.001G256600.v4.1 0 Potri.001G040500.v4.1 0 Potri.001G416900.v4.1 0 Potri.001G452600.v4.1 328 SRR26075393 completed mapping pipeline successfully