Starting /dee2/code/volunteer_pipeline.sh SRR26075394 current disk space = 3051300343808 free memory = 1467519692 SRR26075394 SRAfilesize 6dddff81c7e84c364330e8c1276b95f2 SRR26075394.sra SRR26075394.sra file validated SRR26075394 is paired end SRR26075394 is conventional basespace SRR26075394 read1 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR26075394_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.62 37.0 37.0 37.0 37.0 37.0 2 36.641 37.0 37.0 37.0 37.0 37.0 3 36.6655 37.0 37.0 37.0 37.0 37.0 4 36.652 37.0 37.0 37.0 37.0 37.0 5 36.744 37.0 37.0 37.0 37.0 37.0 6 36.6845 37.0 37.0 37.0 37.0 37.0 7 36.6005 37.0 37.0 37.0 37.0 37.0 8 36.6735 37.0 37.0 37.0 37.0 37.0 9 36.679 37.0 37.0 37.0 37.0 37.0 10-14 36.62765 37.0 37.0 37.0 37.0 37.0 15-19 36.6046 37.0 37.0 37.0 37.0 37.0 20-24 36.62 37.0 37.0 37.0 37.0 37.0 25-29 36.533699999999996 37.0 37.0 37.0 37.0 37.0 30-34 36.4794 37.0 37.0 37.0 37.0 37.0 35-39 36.402100000000004 37.0 37.0 37.0 37.0 37.0 40-44 36.4281 37.0 37.0 37.0 37.0 37.0 45-49 36.378 37.0 37.0 37.0 37.0 37.0 50-54 36.320499999999996 37.0 37.0 37.0 37.0 37.0 55-59 36.1783 37.0 37.0 37.0 37.0 37.0 60-64 36.204499999999996 37.0 37.0 37.0 37.0 37.0 65-69 36.203700000000005 37.0 37.0 37.0 37.0 37.0 70-74 36.2044 37.0 37.0 37.0 37.0 37.0 75-79 36.158 37.0 37.0 37.0 37.0 37.0 80-84 36.0777 37.0 37.0 37.0 37.0 37.0 85-89 36.01279999999999 37.0 37.0 37.0 37.0 37.0 90-94 35.9726 37.0 37.0 37.0 37.0 37.0 95-99 35.977799999999995 37.0 37.0 37.0 37.0 37.0 100-104 35.879099999999994 37.0 37.0 37.0 37.0 37.0 105-109 35.8407 37.0 37.0 37.0 37.0 37.0 110-114 35.757999999999996 37.0 37.0 37.0 37.0 37.0 115-119 35.6462 37.0 37.0 37.0 37.0 37.0 120-124 35.6515 37.0 37.0 37.0 37.0 37.0 125-129 35.4475 37.0 37.0 37.0 37.0 37.0 130-134 35.296 37.0 37.0 37.0 29.8 37.0 135-139 35.324600000000004 37.0 37.0 37.0 34.6 37.0 140-144 35.1711 37.0 37.0 37.0 29.8 37.0 145-149 35.1647 37.0 37.0 37.0 27.4 37.0 150-151 34.900999999999996 37.0 37.0 37.0 25.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.0 1101 2 0.0 1101 3 0.0 1101 4 0.0 1101 5 0.0 1101 6 0.0 1101 7 0.0 1101 8 0.0 1101 9 0.0 1101 10-14 0.0 1101 15-19 0.0 1101 20-24 0.0 1101 25-29 0.0 1101 30-34 0.0 1101 35-39 0.0 1101 40-44 0.0 1101 45-49 0.0 1101 50-54 0.0 1101 55-59 0.0 1101 60-64 0.0 1101 65-69 0.0 1101 70-74 0.0 1101 75-79 0.0 1101 80-84 0.0 1101 85-89 0.0 1101 90-94 0.0 1101 95-99 0.0 1101 100-104 0.0 1101 105-109 0.0 1101 110-114 0.0 1101 115-119 0.0 1101 120-124 0.0 1101 125-129 0.0 1101 130-134 0.0 1101 135-139 0.0 1101 140-144 0.0 1101 145-149 0.0 1101 150-151 0.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 18 1.0 19 2.0 20 0.0 21 0.0 22 1.0 23 4.0 24 9.0 25 4.0 26 5.0 27 12.0 28 23.0 29 20.0 30 34.0 31 27.0 32 42.0 33 103.0 34 144.0 35 461.0 36 2906.0 37 202.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 39.37875751503006 15.731462925851702 7.940881763527054 36.948897795591186 2 19.650000000000002 14.95 33.675 31.724999999999998 3 19.175 18.3 28.499999999999996 34.025 4 21.224999999999998 23.525 26.724999999999998 28.525 5 24.425 29.325000000000003 25.2 21.05 6 21.875 33.5 21.55 23.075000000000003 7 15.675 27.500000000000004 39.85 16.975 8 18.224999999999998 27.025 31.75 23.0 9 19.275000000000002 21.775 37.15 21.8 10-14 20.186009300465024 29.42647132356618 27.38136906845342 23.006150307515373 15-19 19.86 28.134999999999998 27.700000000000003 24.305 20-24 20.369999999999997 26.97 28.615000000000002 24.044999999999998 25-29 19.814999999999998 27.04 28.37 24.775 30-34 20.560000000000002 27.655 27.67 24.115000000000002 35-39 20.34 27.815 28.365000000000002 23.48 40-44 21.02 28.205000000000002 26.85 23.925 45-49 20.68 28.02 27.555000000000003 23.745 50-54 20.145 27.034999999999997 27.944999999999997 24.875 55-59 20.580000000000002 27.61 27.49 24.32 60-64 21.11 28.055000000000003 28.23 22.605 65-69 20.674999999999997 27.91 28.199999999999996 23.215 70-74 21.275 28.34 26.740000000000002 23.645 75-79 21.75 28.244999999999997 26.174999999999997 23.830000000000002 80-84 20.68 27.99 27.315 24.015 85-89 20.26 27.689999999999998 27.889999999999997 24.16 90-94 21.95 27.6 26.700000000000003 23.75 95-99 21.575 26.545 27.865000000000002 24.015 100-104 20.775 27.810000000000002 27.255000000000003 24.16 105-109 21.385 27.025 27.889999999999997 23.7 110-114 20.95 27.310000000000002 27.875 23.865 115-119 21.04 27.779999999999998 27.36 23.82 120-124 21.815 28.08 27.13 22.975 125-129 20.97 27.37 27.61 24.05 130-134 21.11 27.735 27.325 23.830000000000002 135-139 22.235 27.26 26.645000000000003 23.86 140-144 22.035 26.150000000000002 27.145000000000003 24.67 145-149 21.565 27.175 27.115000000000002 24.145 150-151 21.7 27.450000000000003 26.5375 24.3125 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 1.0 9 1.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.5 22 1.0 23 2.0 24 2.5 25 1.5 26 2.5 27 2.5 28 7.5 29 15.0 30 11.5 31 13.0 32 20.5 33 25.5 34 35.5 35 61.5 36 84.0 37 83.5 38 103.5 39 140.0 40 175.5 41 205.0 42 232.5 43 272.5 44 299.0 45 299.5 46 268.5 47 262.0 48 261.0 49 207.5 50 156.5 51 141.5 52 129.0 53 100.5 54 75.5 55 55.5 56 41.0 57 36.5 58 38.0 59 29.0 60 21.0 61 17.5 62 9.5 63 8.0 64 5.5 65 2.0 66 6.0 67 6.5 68 7.5 69 9.0 70 3.0 71 0.0 72 1.5 73 1.5 74 0.0 75 0.0 76 0.0 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.005 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150-151 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 58.15 #Duplication Level Percentage of deduplicated Percentage of total 1 60.79105760963026 35.35 2 21.75408426483233 25.3 3 9.37231298366294 16.35 4 4.514187446259673 10.5 5 1.8486672398968185 5.375 6 0.8598452278589854 3.0 7 0.3869303525365434 1.575 8 0.21496130696474636 1.0 9 0.04299226139294927 0.22499999999999998 >10 0.21496130696474636 1.325 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CCCATTTTCCACTTGATATTCGGAAAGGAGATGTTCATCCTTCAAAACCC 13 0.325 No Hit TTCTTCGCTAGTTTCCTTCAGATTCTTGTCCAATGTAGATACTTGCTCTT 10 0.25 No Hit AGGGTAAGGCAAAGCGGTAGCCATCTTAGGTGTGCGGGTTGATGAGGTGG 10 0.25 No Hit CTCTAGAGAAGTATTTCTGTGTCAAGCATAGAGAGAAGGGAGCACCTTTT 10 0.25 No Hit AGATGATTTTACAGCGTTTGCCATCAGGAAGCTTTTCCTTCCAAATAATT 10 0.25 No Hit CACCCGGCGGCATGGCATGGGGCATCAAGTAGATGCTTCCCTAAACTTGG 9 0.22499999999999998 No Hit GCTTGAAGTTCCTTGTAAGCTTCCCTCTTGGGCCTTCGACTTCGATGATT 8 0.2 No Hit CATGACAAATCGACACTTAATTGTTTCCATAGCAAAATGAAAATTTAAAT 8 0.2 No Hit CAGGCATGGACACCGCTTCCAGGACCATCCAATGCTCGCATCCTAGTCAA 8 0.2 No Hit GCATATCATTCTCAATGACCTCAGTCTTCTGCAAATCTTGATGCAATCCA 8 0.2 No Hit CAATGTTTTAGGTCCATCTGGACCTGCCCGCCATTGTGGAGTCTTTTCAA 8 0.2 No Hit CTCATCCACCAGGGCCTGACGTTCTGCAGGAGTGCCAAAAGTTAAGCATT 7 0.17500000000000002 No Hit CTGCAATGCAGCATCTTCCCAATCTTCCCAAACTACGATGGTATTACTGG 7 0.17500000000000002 No Hit ACACAAATTAAACCAGCACACTGGGCTTGTCGCTCTTCTCAGCTAGCGCA 7 0.17500000000000002 No Hit ATTGGCAATACATTAACGGGCAAATTTACCTCATCAGAAATCCCCATTGG 7 0.17500000000000002 No Hit GGGAGGAAGAAAGTGTCTAGAAACTGGCAGATTGGTTGGTAGTATAGCAG 7 0.17500000000000002 No Hit CCTCAGAATTCACATAGATATGATTGACTCGAAGATAGAGATTTGTAGCA 7 0.17500000000000002 No Hit TGATGCGGTTATGAGTACGACCGGGCGTGGGAGGCACTCGGTCCTCCGGA 7 0.17500000000000002 No Hit AGAAGAAAGGAACTGGATGGTCAAGAGAAGGATAATGGGCAAGAGTAACA 7 0.17500000000000002 No Hit GCTCTCTTATTCTGAAACCATATCGCAACCTGTCGTGGTTGCAACCCAAG 7 0.17500000000000002 No Hit ATTTGCCATTGATAAAGTCCCAGGTCCAGTGTGCTTCAACTTGAAATTCT 6 0.15 No Hit AGCCGCAGTTGCACCCACTCTTCCATGGGCTGTTGCTCTTGTTTTCAGAA 6 0.15 No Hit GGCCTCCTCTAATTTAGATATCTGTTCCAACAATGGTTGAAAATGCTCAT 6 0.15 No Hit ATGGCATTGAAAAGACCTGAGGGAATTTTTCCAGTCAAAGACAATTCCTG 6 0.15 No Hit ATTCAACAAACATTTACTAACAATACAAAGCTGAGTCAAAGCCGGAAATA 6 0.15 No Hit TTTCCATGCCATCCCCAACGGTTGTCATTGTTGGGTCATCTGTCAAAACC 6 0.15 No Hit CGGACCTTCAGGGAGGAGGAAAGCATTGCAACTTTTGTCCCAAAAACACA 6 0.15 No Hit GCCAGACTGAGCTTCGTGTACTAATTAAGCATCCCGACGAACAAGGTAAA 6 0.15 No Hit CCTTCAAATTCCTTTAGTTGTCCCACGGCATATTCATCAATTGCATCGAC 6 0.15 No Hit ATGACACGGAAGATGAAATGATTTTCTTCTTGAATCAAGCAGGACCATTT 6 0.15 No Hit CTCCTCAGTTTCATAAGAAACACATGGCGAGCACATAGCCAGAACATTAT 6 0.15 No Hit CAGCAAGCAACCTAAACCGGAGAACCAAGACCTAAAAAAAGCAAAGAGGG 6 0.15 No Hit CACTGAATGTCAATTCCATTTTGCTTGGCATACCTCATCACAGGCAAATT 6 0.15 No Hit GTTTGATTTGTGACAGCATGCACCGAACATCCTTACTGTTAGGCCTCTGT 6 0.15 No Hit CAGGCATCCAAATCTATACATCGGAGGCAAACCAGCAATGTTCGTTTCAA 6 0.15 No Hit GGAGGAGGTGGTGGAGGCGAAGATTGTGGATCGTCAGTGGGTGGCTTATC 6 0.15 No Hit GCAATTTCTACCGGAATAGACTGAGGTGCACGCCTAGTGAGAGATGCTTC 6 0.15 No Hit CAAGTAGTTCCTATCTAGTGAGGGATCATTAATAGCAGAGAGCTGGGTAG 6 0.15 No Hit CCTTCTTCTTCTCTGAACACTTCTCAAATGAACCATAAATTGAATCAAGC 6 0.15 No Hit GGTGAATTGGTCCAAAATAAGCAATATATGGGCATTGATGAGTAGCAGAT 6 0.15 No Hit GCTGGCATGTGCATGTAAAAGGGCTGTGCCATTTTCAGTGATTTAAGGTC 5 0.125 No Hit GCCTCAAATATGTTTGCACACCTATCATCATTGAACAACACACCAAAAGT 5 0.125 No Hit AGCAGCAGTAGTGACAGTTATAGCAGCCTTTCTCCTGCTACGAGCATATC 5 0.125 No Hit TCCGTCAAAACGCTCCCGTTTCGATCCCTGTTGGAATTGACGGCCACTAC 5 0.125 No Hit CAGCAAACAAAGCAACTTCTGTTCGACTATGTGAAGGAGATTGTTGAGAC 5 0.125 No Hit ACCATCCGTCGCGGAACTCCTCCAGCATACGAACCGAATCCCCAACGTCA 5 0.125 No Hit ATAATTTTTTCCTTGTTGATTTCATGAACTATGGCATAAGCTAAGGCTAT 5 0.125 No Hit GTCCTTTCCTTTCACACTGGACAACAGCAGCTCAATCCTGTCATCGTCAG 5 0.125 No Hit CCTAAGTCGGGCACGGCGAGGTGCCCTCTCGGACTATAAGCACAAGGCAG 5 0.125 No Hit CAAGCACTTCAGCTTCTACTCTTGTGCCATAGAATTTTGGAACAGGTTGT 5 0.125 No Hit GCCCAAACCTTTTATCATTGGCTATCATTGCTGCCAAGGAACCCAAATCT 5 0.125 No Hit TTGTGATTGTTATTATTATTGTTATTATTCTCCAAGGCAGGCGAATCGGA 5 0.125 No Hit GCCCACTGGGAAGACCAAATTTTGGCAAGATTTCAAAGACTGTAGGAGAA 5 0.125 No Hit CTCTCTATCAGTTTCTCCTCCATCGCCCCCTCCCAATCCCTCTCTTTCTC 5 0.125 No Hit TCCTCCTCTGGTGCCATGAGTGTAGCTTCTTTTGTAACAAGACCTTCAAG 5 0.125 No Hit CAAGAAGTTCTTCTGCAGATGATTTTGATGATATCTGTGAGAAAGGGACG 5 0.125 No Hit GGAGATTGGAGAGAGTGGTCTGACAAGCGCGGTTAACATCGAGTGGAGTA 5 0.125 No Hit GCGTAGATTAGGGAAGTTAAGCCTTGCAAAGTCGCCTCTGAGTTTGTAAG 5 0.125 No Hit GAGCAGGTAGGATCTCCCAAGGTCTTTTTGCCCTATCCATATCGCCTTTC 5 0.125 No Hit GCTTGTCCTTCATCTGGCCAACAAACCCTTCCTGGCGCTCACCTTGTGCG 5 0.125 No Hit GGTGAGGAAGGAGGTGGAAGTAATTGGTCGCAACATAATAGAAGATAGAG 5 0.125 No Hit GTTTTTGATACCCAATATGAGTTGTCCGCAAGCTTGGTTCCCATTTCTTC 5 0.125 No Hit AAGGATAACAAATTTAGTATATATGCAATGGTCAATTGCAAACAAGATGA 5 0.125 No Hit AGTACTTCTTGAAGAAATAACCAAAACACCTCATCACAAAAGGCACTGAA 5 0.125 No Hit CTCTTGAATAGTGCACAAAATATTGTGGGACTGCTGGCTTGGGTCAGAAA 5 0.125 No Hit CTCCAGATTGGCTTCCAGCAATAAGGACACAATTGTTGACAGACACCCCA 5 0.125 No Hit CGGCTTTCGTCTTCTCATGTTTGAGCCAACTATGATGTGATTGTTTGGGA 5 0.125 No Hit GAACAATACTGGGGAACAGAAAACGAACAACTGCTGGTGGCCTGCTTCTT 5 0.125 No Hit GGCCGTCATCGCCTGTAACAATGTTTCCAACTGTTCGAAGGGCAGGAATA 5 0.125 No Hit GCCTTCTCATCCTTTGCAACCAAGATATTGTCAAACAATATGCCATCCTG 5 0.125 No Hit GCACCATCAGTGAGAAATATGAGTTCTGGGTCACTTGGATATCCATCACG 5 0.125 No Hit ACCAAAGCTGTACACGTCACTCTTTTCATCAACCTTCAGTGTGTAGGCAT 5 0.125 No Hit TCATCGGATGGCACTCCACTGTTGGGCAATGGAGGTGTGGTTTTACCCCA 5 0.125 No Hit CGGGACGAGTCGAGGACTGTCTCTACCGAGTTCTCCCGCTGTCCAGGGTT 5 0.125 No Hit CTTCTAGAGTTCAAAATCTGTCATAACACCACATATTCAGTTTAGTTTCA 5 0.125 No Hit CTTCAGTGTAGTGAAATCAACATGGTAAAGTCCGTATCTTTGTGTATAAC 5 0.125 No Hit GTTCGAAACGCGGCATTTTTATCAACTACACAAAAACACGCACTTGGATA 5 0.125 No Hit CTTAGAGACACGAACACTTGTGAATGGATTGATGTTCCAATAACGAATCT 5 0.125 No Hit GTAGCATTTGCTTTCACAAGCCGAGTCACTTCCTGTGTAATTTGTTCACA 5 0.125 No Hit GCATTCTCTACTGCCATTTTCAACTAGCATTAGATCTTTCGATCCATTCC 5 0.125 No Hit CCCAGATTAGCAAGCCAGTCTTACCCCCAACATAAACATCACCACTTGGA 5 0.125 No Hit ATAGACCTAGTGCCAGCAATACCAACAAGTAGATCAAGCTTAGCTTGATC 5 0.125 No Hit TTCTGCTTTGTTATATTGTTCTCGGTCTGGAACCACAGTTGTCTTATCCT 5 0.125 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.037500000000000006 0.0 0.0 0.0 0.0 64-65 0.05 0.0 0.0 0.0 0.0 66-67 0.05 0.0 0.0 0.0 0.0 68-69 0.25 0.0 0.0 0.0 0.0 70-71 0.25 0.0 0.0 0.0 0.0 72-73 0.2625 0.0 0.0 0.0 0.0 74-75 0.3 0.0 0.0 0.0 0.0 76-77 0.35 0.0 0.0 0.0 0.0 78-79 0.4125 0.0 0.0 0.0 0.0 80-81 0.425 0.0 0.0 0.0 0.0 82-83 0.425 0.0 0.0 0.0 0.0 84-85 0.425 0.0 0.0 0.0 0.0 86-87 0.5 0.0 0.0 0.0 0.0 88-89 0.575 0.0 0.0 0.0 0.0 90-91 0.625 0.0 0.0 0.0 0.0 92-93 0.7124999999999999 0.0 0.0 0.0 0.0 94-95 0.8125 0.0 0.0 0.0 0.0 96-97 0.9125000000000001 0.0 0.0 0.0 0.0 98-99 1.1124999999999998 0.0 0.0 0.0 0.0 100-101 1.4375 0.0 0.0 0.0 0.0 102-103 1.7625000000000002 0.0 0.0 0.0 0.0 104-105 2.125 0.0 0.0 0.0 0.0 106-107 2.5 0.0 0.0 0.0 0.0 108-109 2.7125 0.0 0.0 0.0 0.0 110-111 3.1625 0.0 0.0 0.0 0.0 112-113 3.375 0.0 0.0 0.0 0.0 114-115 3.7125000000000004 0.0 0.0 0.0 0.0 116-117 4.1125 0.0 0.0 0.0 0.0 118-119 4.4125 0.0 0.0 0.0 0.0 120-121 5.050000000000001 0.0 0.0 0.0 0.0 122-123 5.875 0.0 0.0 0.0 0.0 124-125 6.5 0.0 0.0 0.0 0.0 126-127 7.137499999999999 0.0 0.0 0.0 0.0 128-129 7.7375 0.0 0.0 0.0 0.0 130-131 8.425 0.0 0.0 0.0 0.0 132-133 9.075 0.0 0.0 0.0 0.0 134-135 9.5625 0.0 0.0 0.0 0.0 136-137 10.3375 0.0 0.0 0.0 0.0 138-139 11.162500000000001 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CCTGTAA 10 0.006830828 145.0 1 GTTCACA 10 0.006830828 145.0 145 CCTCCTC 10 0.006830828 145.0 3 AAGTGAG 10 0.006830828 145.0 6 TTTTTTT 20 0.00593511 29.0 115-119 CACACGT 55 1.1668232E-4 18.454546 135-139 GTCTGAA 55 1.1668232E-4 18.454546 140-144 GGGGGGG 40 0.0076550315 18.125 140-144 CGTCTGA 50 0.0013298223 17.4 140-144 GCACACG 55 0.0025160722 15.818182 135-139 ATCGGAA 65 4.1823133E-4 15.615384 125-129 AAGAGCA 70 7.343502E-4 14.5 130-134 GATCGGA 65 0.0076375785 13.384615 125-129 >>END_MODULE SRR26075394 read2 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR26075394_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 35.47225 37.0 37.0 37.0 37.0 37.0 2 35.803 37.0 37.0 37.0 37.0 37.0 3 35.863 37.0 37.0 37.0 37.0 37.0 4 35.868 37.0 37.0 37.0 37.0 37.0 5 35.866 37.0 37.0 37.0 37.0 37.0 6 35.627 37.0 37.0 37.0 37.0 37.0 7 35.6385 37.0 37.0 37.0 37.0 37.0 8 35.8065 37.0 37.0 37.0 37.0 37.0 9 35.798 37.0 37.0 37.0 37.0 37.0 10-14 35.692 37.0 37.0 37.0 37.0 37.0 15-19 35.6666 37.0 37.0 37.0 37.0 37.0 20-24 35.59439999999999 37.0 37.0 37.0 37.0 37.0 25-29 35.5026 37.0 37.0 37.0 37.0 37.0 30-34 35.391000000000005 37.0 37.0 37.0 37.0 37.0 35-39 35.3245 37.0 37.0 37.0 37.0 37.0 40-44 35.2573 37.0 37.0 37.0 37.0 37.0 45-49 35.211 37.0 37.0 37.0 34.6 37.0 50-54 35.0894 37.0 37.0 37.0 34.6 37.0 55-59 35.1505 37.0 37.0 37.0 34.6 37.0 60-64 35.1418 37.0 37.0 37.0 34.6 37.0 65-69 35.0575 37.0 37.0 37.0 29.8 37.0 70-74 35.008 37.0 37.0 37.0 27.4 37.0 75-79 34.8914 37.0 37.0 37.0 25.0 37.0 80-84 35.0364 37.0 37.0 37.0 27.4 37.0 85-89 34.9006 37.0 37.0 37.0 25.0 37.0 90-94 34.75825 37.0 37.0 37.0 25.0 37.0 95-99 34.8361 37.0 37.0 37.0 25.0 37.0 100-104 34.7908 37.0 37.0 37.0 25.0 37.0 105-109 34.7743 37.0 37.0 37.0 25.0 37.0 110-114 34.6456 37.0 37.0 37.0 25.0 37.0 115-119 34.6399 37.0 37.0 37.0 25.0 37.0 120-124 34.396950000000004 37.0 37.0 37.0 25.0 37.0 125-129 34.466899999999995 37.0 37.0 37.0 25.0 37.0 130-134 34.4659 37.0 37.0 37.0 25.0 37.0 135-139 34.2121 37.0 37.0 37.0 25.0 37.0 140-144 34.163650000000004 37.0 37.0 37.0 25.0 37.0 145-149 34.228950000000005 37.0 37.0 37.0 25.0 37.0 150-151 33.779250000000005 37.0 37.0 37.0 25.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.0 1101 2 0.0 1101 3 0.0 1101 4 0.0 1101 5 0.0 1101 6 0.0 1101 7 0.0 1101 8 0.0 1101 9 0.0 1101 10-14 0.0 1101 15-19 0.0 1101 20-24 0.0 1101 25-29 0.0 1101 30-34 0.0 1101 35-39 0.0 1101 40-44 0.0 1101 45-49 0.0 1101 50-54 0.0 1101 55-59 0.0 1101 60-64 0.0 1101 65-69 0.0 1101 70-74 0.0 1101 75-79 0.0 1101 80-84 0.0 1101 85-89 0.0 1101 90-94 0.0 1101 95-99 0.0 1101 100-104 0.0 1101 105-109 0.0 1101 110-114 0.0 1101 115-119 0.0 1101 120-124 0.0 1101 125-129 0.0 1101 130-134 0.0 1101 135-139 0.0 1101 140-144 0.0 1101 145-149 0.0 1101 150-151 0.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 13 7.0 14 16.0 15 17.0 16 24.0 17 13.0 18 12.0 19 10.0 20 13.0 21 11.0 22 10.0 23 19.0 24 16.0 25 13.0 26 20.0 27 24.0 28 17.0 29 28.0 30 31.0 31 46.0 32 81.0 33 125.0 34 265.0 35 835.0 36 2204.0 37 143.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.71092773193298 22.980745186296573 11.40285071267817 21.905476369092273 2 31.324999999999996 26.924999999999997 25.1 16.650000000000002 3 24.349999999999998 29.575000000000003 26.724999999999998 19.35 4 27.650000000000002 32.550000000000004 20.775 19.025 5 28.549999999999997 35.6 18.75 17.1 6 22.675 39.775 21.075 16.475 7 23.474999999999998 24.55 35.475 16.5 8 24.8 23.799999999999997 25.775 25.624999999999996 9 23.775 26.924999999999997 27.800000000000004 21.5 10-14 26.25 29.470000000000002 24.145 20.135 15-19 26.205000000000002 28.810000000000002 25.36 19.625 20-24 25.869999999999997 28.07 26.314999999999998 19.744999999999997 25-29 25.415 28.465 25.374999999999996 20.745 30-34 25.105 29.220000000000002 26.484999999999996 19.189999999999998 35-39 24.884999999999998 29.080000000000002 25.924999999999997 20.11 40-44 24.7 28.384999999999998 26.16 20.755000000000003 45-49 24.95 27.944999999999997 26.615 20.49 50-54 22.795 28.735 27.92 20.549999999999997 55-59 24.275 29.68 25.705 20.34 60-64 24.73 29.080000000000002 24.65 21.54 65-69 24.154999999999998 29.575000000000003 25.755 20.515 70-74 24.39 29.15 26.075 20.385 75-79 23.01 28.994999999999997 26.96 21.035 80-84 24.12 28.895 25.91 21.075 85-89 24.98 27.955000000000002 26.02 21.044999999999998 90-94 24.40622031101555 27.816390819540977 26.77133856692835 21.006050302515124 95-99 24.55 29.145 26.625 19.68 100-104 24.395 28.705000000000002 26.745 20.155 105-109 24.705 28.235 27.845 19.215 110-114 25.990000000000002 29.18 25.064999999999998 19.765 115-119 24.68 29.13 26.224999999999998 19.965 120-124 25.141257062853146 29.546477323866192 25.951297564878246 19.36096804840242 125-129 25.6 28.575 26.265 19.56 130-134 26.26 29.315 25.72 18.705 135-139 25.1000200040008 29.905981196239246 25.580116023204642 19.41388277655531 140-144 25.99909968488971 29.28024808683039 26.129145200820286 18.59150702745961 145-149 27.229976487067887 29.306118365100804 24.678573215268397 18.78533193256291 150-151 26.813406703351678 28.251625812906454 26.075537768884445 18.859429714857427 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.5 5 1.5 6 1.0 7 1.0 8 2.0 9 1.5 10 1.5 11 3.0 12 3.5 13 1.5 14 1.5 15 3.5 16 2.5 17 1.0 18 2.5 19 2.0 20 3.0 21 5.0 22 4.0 23 3.0 24 2.5 25 3.0 26 2.5 27 3.0 28 4.5 29 11.0 30 12.0 31 15.5 32 22.0 33 26.0 34 37.5 35 43.5 36 52.5 37 73.0 38 101.0 39 144.5 40 175.5 41 207.5 42 253.0 43 293.5 44 310.5 45 276.0 46 246.5 47 258.0 48 223.0 49 170.0 50 171.5 51 166.0 52 124.5 53 95.5 54 80.0 55 62.0 56 53.0 57 34.5 58 19.0 59 19.0 60 18.0 61 13.0 62 12.5 63 10.0 64 6.5 65 10.0 66 10.5 67 6.0 68 3.5 69 3.5 70 4.0 71 1.5 72 2.5 73 3.5 74 3.0 75 1.5 76 1.0 77 2.0 78 2.0 79 3.0 80 3.5 81 2.5 82 1.5 83 2.0 84 1.5 85 0.0 86 1.5 87 2.5 88 2.0 89 2.0 90 1.0 91 0.0 92 0.0 93 1.0 94 1.5 95 1.5 96 1.5 97 1.0 98 1.5 99 2.5 100 8.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.025 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.005 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.005 125-129 0.0 130-134 0.0 135-139 0.02 140-144 0.034999999999999996 145-149 0.055 150-151 0.05 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 60.35 #Duplication Level Percentage of deduplicated Percentage of total 1 65.16155758077879 39.324999999999996 2 18.889809444904724 22.8 3 8.864954432477216 16.05 4 3.645401822700911 8.799999999999999 5 1.9055509527754766 5.75 6 0.579950289975145 2.1 7 0.4556752278376139 1.925 8 0.2071251035625518 1.0 9 0.08285004142502071 0.44999999999999996 >10 0.2071251035625518 1.7999999999999998 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG 29 0.7250000000000001 No Hit CTATAGAGCTCAATATCAAGACTTTAGATTCTCAGATTTATAGTTTTCAG 13 0.325 No Hit CGAAGATGGCAGGGAATTTAGTGAAGGAGGATTCCAGTTCGGTTCAGGAT 10 0.25 No Hit TCATGATCATGGACATCGCCGCAAGTTCCGTGACAACGGAAACGTTCCTT 10 0.25 No Hit AAGTCAGTAAACGTGTGCCATCAAACGGTTTGTATTTATGTTCTGTAGTT 10 0.25 No Hit GAAATCCAAGAGCAAGAGTGTTGGTGGAGTCAGGAAAATTTCATAACGAG 9 0.22499999999999998 No Hit GTAGGAGGTGGCTTTGGCGGTGATGGTGCTGCTTTTGGTGCCGTGAATAT 9 0.22499999999999998 No Hit GGAGGAGATGCTGGAAACCGTGAGAACTACATTAATGAGCTTATCAGGAG 8 0.2 No Hit CAGATGTTGGCTTTTCCTATATAGCAGCAAAAAGTCCAGGAGAAGGATCA 8 0.2 No Hit ATCAAGATCAATGCTAGAATCATCGAAGTCGAAGGCCCAAGAGGGAAGCT 8 0.2 No Hit CAAGATTCATGATGGCAATGAGCATGTTAGCCTTGCATCTTGTCAGGGAA 8 0.2 No Hit GCCGATTTGTAGAGATTAGAGATTCTAAGTGGTGGTGTACAATCTGCAAT 8 0.2 No Hit GGGGCCTCGGGAAGAGTTATCTTTTCTGTTTAACAGCCCGCCCACCCTGG 7 0.17500000000000002 No Hit TCTGACTTGTAATTACTCAATTGTTCTGAATTCAGTACATCTAAATTCAG 7 0.17500000000000002 No Hit GAATACTACTAGCAGTAGTAGTATCCATAGTGGTGCTAGCTCCATTCATC 7 0.17500000000000002 No Hit TGGGAAACCACATGAGCGTTCAGCAATAATAAAGAAATTAACTGGGCAGA 7 0.17500000000000002 No Hit ACCAGATGAAACTTACTGTAATTGGGTTAAGAAGTTGGAAAAGAGACTTG 7 0.17500000000000002 No Hit GTTCATACTAAAAGGAATTGCTGAGAAGATTTTCTGGCAACCGGGTCCTG 7 0.17500000000000002 No Hit AAGATATCGATGCAAGTCTTACGAAGGCATAATAATGGAAAGAAGAGACA 7 0.17500000000000002 No Hit GTCACAACCTCCAGAAAGAAGAAGAACAAGATTAAAAGGAGGTTTAGCGA 7 0.17500000000000002 No Hit GAGAGATATAGGGGAAAAGAAAGCTAAAAAGGAAAAGAAGTTTCAAGTAC 7 0.17500000000000002 No Hit ATCCAGCAGTTTCTGGAGGAGGAGCCATTGGTCTTGGTGCTCCATCTATC 7 0.17500000000000002 No Hit GGCCAAAGAAGCGAGTCAACTGACAGCGGTCACCACAAGGACCACAAATG 7 0.17500000000000002 No Hit CATTTCTTCTCTGCTTTTTCCCCCAAAATTTTAAGCCCTCAAAATTTCCT 6 0.15 No Hit CCATGGATGACTGGGCACATGATGAGGACCTTTATGACTTAAGTTACTCT 6 0.15 No Hit GTGCTAGATACTAGTAGTAGTTGCAGACAGAGAGCTAGTGTTAGACAGGT 6 0.15 No Hit GACCTACGCTGGTTAGTAGACAGCCCTACCTGGGGCAGCTAGAGACCATC 6 0.15 No Hit ACTCAATTAGCAGGATACAATCACAACTTTTGAAACTATCTGTGCGGAGG 6 0.15 No Hit TTTGGAAGAGAAGATGTCAGGCAAAGGAGCAAAGGGATTGTTAACTAGCA 6 0.15 No Hit ATGGAAAACCAGCTGGTCGCATTACCATGGGACTATTTGGCAAGACAGTT 6 0.15 No Hit ACTTGGTTGATTGGTTCAAAGGAATGGTAGCAAGCCGTCGTGGAGAGGAG 6 0.15 No Hit GAAATATTATGATATCAGAGAATCCAAGTCAAGCTATTGATCCAAAGCTG 6 0.15 No Hit AGAAAATTGAGTTTGTGGCACATCAGAAAGGAGTCCACCGTTTTTGCTTC 6 0.15 No Hit TACAAGTCATAACAGGTGTAGGCAACCATAGCCGGGGCCAAGCTGCACTC 6 0.15 No Hit AGAAAGAAGAAATGAAGCAGAGAAACTGAGTTTGGAGCAAAAGATGTTAG 6 0.15 No Hit GGACAACAAGTGGTGGAGCTTCAGGAGGAGCAGCTGGGACCATGCTTCAG 6 0.15 No Hit GTGAATGAGTCTGTTTACCTTTGCACTACCAAGCCTTTGAGTGAGCATGA 6 0.15 No Hit GTTTCAGCCCTCCACGCGACGGACGAGAAAGAAAATATTGAGCTGATTTG 5 0.125 No Hit ATTGCAAAAGCTAAGCATTATCTTGCTATGACTACTGGTGGTCTAGGTGC 5 0.125 No Hit CCACAAAAGGTCTATGATTTCGTTCTGATGCTGATGAAGAAGTTTGGTAT 5 0.125 No Hit CAGACTCTCATCAGAAAAGCTTAGACTCATCCGATTTAGGGTTTCGCTTC 5 0.125 No Hit CTTAGACTCACCAAAGAGCAATCTGCCCTTTTGGAGGAAAGCTTCAAACA 5 0.125 No Hit TACCACTTAAGTGCATTGCTTGGAGCCTATGCAGTTTTCATCCGACAGAC 5 0.125 No Hit GCGGTAGAATGTCTGTTCCTCCTTCCTCTGCTAAGGTGGAATCTGATGTT 5 0.125 No Hit AAGCAAGCGAATGATGACCAGATGAAATTGAAGATTCAAGCACAACATCA 5 0.125 No Hit GCATGAAATTCTAAAGAAGAAAAATGTGCAGGAGAAGCACAACAGACCTG 5 0.125 No Hit CAGCTGGTTCTACTCCTCCGGTGTCTCCTTTCTCTGGAAGCAGAGAGTCC 5 0.125 No Hit CTATGAACCTCCTTATTTGGATATTGGGGATGGTGTCACTTATAGGATAC 5 0.125 No Hit TGAACAGAAGTTGAGAGAGGTACTAAGATTTGCAAATGCATGTGGAGCCA 5 0.125 No Hit CCCAAACCAGATCCACCAGATCCAAACCCAAATCCACCAAAATAACAGCC 5 0.125 No Hit GGAAGCCCCTGGTTGTGGTGAATGGAAGAAACCAATGAAGAGAAATCCAG 5 0.125 No Hit AAATATTTCTCCAAAATTTAAGCTACCATGGGGGAAGAAGTGAAAATGAG 5 0.125 No Hit GATTCTCACGCGATCCGGCCTGGAAAGGGGAAAATGAGGAACAGGAGGTA 5 0.125 No Hit AAAAAAGAAGGGAAGAGAGAAAGAGAGAAACCATGAATGTTGAAGAAGAG 5 0.125 No Hit AAATGGACATGGGTGGTTAGCTATCGACAGCAAATACAAACCCAAGGGGC 5 0.125 No Hit TCTTTTTCTCCGAAGAATCTCTCTCTCGCTCTCTCAAATCCAAACCCCCC 5 0.125 No Hit GAGAGAGAACATTCTCTCATTGATTACACGCATAAAGCATCCACAAGCTT 5 0.125 No Hit GTTCATTATCCTCGCATCAGACGGGGTGTGGGATGTGCTTAGCAACAAGG 5 0.125 No Hit GGTTGATGCCTCTGGGAGGAAGGGCAAGGGAAAGGGAGTGTACCAATTTG 5 0.125 No Hit AAACGACAAAATTCAGGCTGTGATTGATGCTGGTGTCTGTCGACGTCTGG 5 0.125 No Hit CTGGAACAAATCTAACTGCTGCTTCGTTGGAGTCTTATAATCTTGGGAGA 5 0.125 No Hit CTTGGATCGATCTGAGAGGTAGAAGCTCCATTGCGCAATCATGTTGGTCT 5 0.125 No Hit GATTGCTGTTGAGGCTGCAAAGGGCCTTTGCTACCTTCATCATGATTGCT 5 0.125 No Hit GGCATAAATTACCAAAATTTGCCGTCTTGCATACCGAGAAGATCGAAATG 5 0.125 No Hit TAAAGGAATCTTGTCTGGTACGAATGAGGAACTAGCTAAGGAAGCAATGA 5 0.125 No Hit GATATCACTCGACTAAGAGTGGTGATGAGTTGACCAGCCTGAAGGATTAT 5 0.125 No Hit GCTACAAATGCGGAGAATGGTTACAATCCGACAAGATCTGGTCGGGCCGG 5 0.125 No Hit TGAAATGACCGGGAGGAGGAGAGTGGTTTCTGCTTGTATGGGTCAAGAGG 5 0.125 No Hit TTCATACCATCTTTCTCTGTTGAGGTCTTCAAGCAGCTTAATTTGCCTCT 5 0.125 No Hit GGACAAGCTCCCGGGCGGGGGCGGTGGCGGTGGCGGTGGCATGACGCAAG 5 0.125 No Hit GGCACCTGAATTAACATACGACGTGCCCGAACTCGTCATGTCTCGCAAGT 5 0.125 No Hit AGAAGATCTAAGAAAAATGTCGGCAGTTAGCGTAGAGCCTTTCCTTCGAT 5 0.125 No Hit GATCATTACAGAGAATGGGTATGGCCAGCAGAACAATCCAAACCTCACTA 5 0.125 No Hit GGCTGTTTTTGGACTCCTCAGGATTTGCCAGCGGCTGCTGCCCTACAAAG 5 0.125 No Hit GGACATGACAAGAAGGTTGTCCTGTGGAACATGGATAACCTGCAAACAGA 5 0.125 No Hit CATGCCTGTGTTTCCTGGCAACAAGGATGAAAAAGTATCAAGGTGTTGGA 5 0.125 No Hit GTGCAGAAAGGGAGAGGAGGAGAAAACGTCCCAGATCGCCCTGCCTCTTG 5 0.125 No Hit GCATTCCTGTGCGTTATGAAGCATTTTGGAAGGAGCTTGATTATGCTAAG 5 0.125 No Hit ATGAATAGAAATGAATCATCAAGTTATATTTTGGTTGCCAATATTTACAA 5 0.125 No Hit CACGTCTGTCACCAATCCACCTAATCCACCTACACCTCCAGTCGGCACCA 5 0.125 No Hit GTTGCATTTATCTAAAGTATTCTCACTTTACTTAACACTTGAGCTACATA 5 0.125 No Hit GACTCGTGGAAGAGGCAAGTGGGAGGTGATTGAGAAACCAAGATTTGGTT 5 0.125 No Hit ATCCTGAAAGACTCTCTTCCCTGTGTTTGTTTCTTCTCTTTATTGAAAAT 5 0.125 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.025 0.0 0.0 0.0 0.0 60-61 0.025 0.0 0.0 0.0 0.0 62-63 0.0625 0.0 0.0 0.0 0.0 64-65 0.075 0.0 0.0 0.0 0.0 66-67 0.075 0.0 0.0 0.0 0.0 68-69 0.275 0.0 0.0 0.0 0.0 70-71 0.275 0.0 0.0 0.0 0.0 72-73 0.2875 0.0 0.0 0.0 0.0 74-75 0.325 0.0 0.0 0.0 0.0 76-77 0.375 0.0 0.0 0.0 0.0 78-79 0.4375 0.0 0.0 0.0 0.0 80-81 0.45 0.0 0.0 0.0 0.0 82-83 0.45 0.0 0.0 0.0 0.0 84-85 0.45 0.0 0.0 0.0 0.0 86-87 0.525 0.0 0.0 0.0 0.0 88-89 0.6 0.0 0.0 0.0 0.0 90-91 0.65 0.0 0.0 0.0 0.0 92-93 0.7375 0.0 0.0 0.0 0.0 94-95 0.8374999999999999 0.0 0.0 0.0 0.0 96-97 0.9375 0.0 0.0 0.0 0.0 98-99 1.1375000000000002 0.0 0.0 0.0 0.0 100-101 1.4625 0.0 0.0 0.0 0.0 102-103 1.8 0.0 0.0 0.0 0.0 104-105 2.175 0.0 0.0 0.0 0.0 106-107 2.55 0.0 0.0 0.0 0.0 108-109 2.7375 0.0 0.0 0.0 0.0 110-111 3.1625 0.0 0.0 0.0 0.0 112-113 3.375 0.0 0.0 0.0 0.0 114-115 3.7 0.0 0.0 0.0 0.0 116-117 4.0875 0.0 0.0 0.0 0.0 118-119 4.375 0.0 0.0 0.0 0.0 120-121 5.0 0.0 0.0 0.0 0.0 122-123 5.824999999999999 0.0 0.0 0.0 0.0 124-125 6.475 0.0 0.0 0.0 0.0 126-127 7.137499999999999 0.0 0.0 0.0 0.0 128-129 7.75 0.0 0.0 0.0 0.0 130-131 8.45 0.0 0.0 0.0 0.0 132-133 9.1 0.0 0.0 0.0 0.0 134-135 9.5875 0.0 0.0 0.0 0.0 136-137 10.3625 0.0 0.0 0.0 0.0 138-139 11.162500000000001 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACAGGA 10 0.006830828 145.0 145 GTGAACC 10 0.006830828 145.0 1 CAAAAGC 10 0.006830828 145.0 2 TGTAGGG 50 5.60876E-5 20.3 140-144 CGTCGTG 55 1.1668232E-4 18.454546 135-139 GTGTAGG 50 0.0013298223 17.4 140-144 AAGAGCG 60 2.2715089E-4 16.916668 130-134 GCGTCGT 55 0.0025160722 15.818182 135-139 ATCGGAA 65 4.1823133E-4 15.615384 125-129 GTCGTGT 60 0.004491891 14.500001 135-139 GATCGGA 65 0.0076375785 13.384615 125-129 GAAGAGC 65 0.0076375785 13.384615 130-134 >>END_MODULE Read 1112504 spots for SRR26075394.sra Written 1112504 spots for SRR26075394.sra Read 1112504 spots for SRR26075394.sra Written 1112504 spots for SRR26075394.sra Read 1112504 spots for SRR26075394.sra Written 1112504 spots for SRR26075394.sra Read 1112504 spots for SRR26075394.sra Written 1112504 spots for SRR26075394.sra Read 1112504 spots for SRR26075394.sra Written 1112504 spots for SRR26075394.sra Read 1112504 spots for SRR26075394.sra Written 1112504 spots for SRR26075394.sra Read 1112504 spots for SRR26075394.sra Written 1112504 spots for SRR26075394.sra Read 1112504 spots for SRR26075394.sra Written 1112504 spots for SRR26075394.sra Read 1112504 spots for SRR26075394.sra Written 1112504 spots for SRR26075394.sra Read 1112504 spots for SRR26075394.sra Written 1112504 spots for SRR26075394.sra Read 1112504 spots for SRR26075394.sra Written 1112504 spots for SRR26075394.sra Read 1112504 spots for SRR26075394.sra Written 1112504 spots for SRR26075394.sra Read 1112504 spots for SRR26075394.sra Written 1112504 spots for SRR26075394.sra Read 1112504 spots for SRR26075394.sra Written 1112504 spots for SRR26075394.sra Read 1112511 spots for SRR26075394.sra Written 1112511 spots for SRR26075394.sra Read 1112504 spots for SRR26075394.sra Written 1112504 spots for SRR26075394.sra Read 1112504 spots for SRR26075394.sra Written 1112504 spots for SRR26075394.sra Read 1112504 spots for SRR26075394.sra Written 1112504 spots for SRR26075394.sra Read 1112504 spots for SRR26075394.sra Written 1112504 spots for SRR26075394.sra Read 1112504 spots for SRR26075394.sra Written 1112504 spots for SRR26075394.sra SRR ids: ['SRR26075394.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_dgnzc4ri SRR26075394.sra spots: 22250087 blocks: [[1, 1112504], [1112505, 2225008], [2225009, 3337512], [3337513, 4450016], [4450017, 5562520], [5562521, 6675024], [6675025, 7787528], [7787529, 8900032], [8900033, 10012536], [10012537, 11125040], [11125041, 12237544], [12237545, 13350048], [13350049, 14462552], [14462553, 15575056], [15575057, 16687560], [16687561, 17800064], [17800065, 18912568], [18912569, 20025072], [20025073, 21137576], [21137577, 22250087]] SRR26075394 file size 8212673 SRR26075394 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o SRR26075394 SRR26075394_1.fastq SRR26075394_2.fastq Input file: SRR26075394_1.fastq Paired file: SRR26075394_2.fastq trimmed: SRR26075394-trimmed-pair1.fastq, SRR26075394-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Wed Feb 12 00:45:18 2025 >> started Wed Feb 12 00:45:47 2025 >> done (28.656s) 22250087 read pairs processed; of these: 100 ( 0.00%) short read pairs filtered out after trimming by size control 75265 ( 0.34%) empty read pairs filtered out after trimming by size control 22174722 (99.66%) read pairs available; of these: 3437941 (15.50%) trimmed read pairs available after processing 18736781 (84.50%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 18 6 0.00% 19 7 0.00% 20 30 0.00% 21 13 0.00% 22 13 0.00% 23 7 0.00% 24 20 0.00% 25 12 0.00% 26 15 0.00% 27 15 0.00% 28 38 0.00% 29 21 0.00% 30 22 0.00% 31 25 0.00% 32 27 0.00% 33 21 0.00% 34 35 0.00% 35 35 0.00% 36 32 0.00% 37 35 0.00% 38 37 0.00% 39 42 0.00% 40 45 0.00% 41 61 0.00% 42 73 0.00% 43 90 0.00% 44 70 0.00% 45 89 0.00% 46 74 0.00% 47 83 0.00% 48 92 0.00% 49 96 0.00% 50 130 0.00% 51 132 0.00% 52 165 0.00% 53 175 0.00% 54 185 0.00% 55 245 0.00% 56 231 0.00% 57 245 0.00% 58 276 0.00% 59 338 0.00% 60 380 0.00% 61 506 0.00% 62 538 0.00% 63 617 0.00% 64 645 0.00% 65 741 0.00% 66 788 0.00% 67 948 0.00% 68 1215 0.01% 69 1257 0.01% 70 1415 0.01% 71 1620 0.01% 72 1996 0.01% 73 2134 0.01% 74 2403 0.01% 75 2788 0.01% 76 2976 0.01% 77 3299 0.01% 78 3854 0.02% 79 4219 0.02% 80 4714 0.02% 81 5250 0.02% 82 6024 0.03% 83 7045 0.03% 84 7787 0.04% 85 8601 0.04% 86 9249 0.04% 87 10264 0.05% 88 10931 0.05% 89 11841 0.05% 90 13012 0.06% 91 13960 0.06% 92 14797 0.07% 93 16523 0.07% 94 17741 0.08% 95 19212 0.09% 96 20797 0.09% 97 21322 0.10% 98 22576 0.10% 99 23939 0.11% 100 25117 0.11% 101 25469 0.11% 102 27428 0.12% 103 29445 0.13% 104 31125 0.14% 105 33301 0.15% 106 34688 0.16% 107 35949 0.16% 108 37145 0.17% 109 38455 0.17% 110 39161 0.18% 111 40912 0.18% 112 41877 0.19% 113 42964 0.19% 114 45983 0.21% 115 47521 0.21% 116 48830 0.22% 117 50209 0.23% 118 51927 0.23% 119 52814 0.24% 120 53540 0.24% 121 55175 0.25% 122 56267 0.25% 123 58573 0.26% 124 60743 0.27% 125 62711 0.28% 126 63917 0.29% 127 65867 0.30% 128 67505 0.30% 129 69807 0.31% 130 70148 0.32% 131 70985 0.32% 132 71356 0.32% 133 74110 0.33% 134 74582 0.34% 135 76282 0.34% 136 77866 0.35% 137 78611 0.35% 138 81508 0.37% 139 82637 0.37% 140 84167 0.38% 141 84138 0.38% 142 85519 0.39% 143 86222 0.39% 144 88778 0.40% 145 89486 0.40% 146 91229 0.41% 147 91265 0.41% 148 93228 0.42% 149 92881 0.42% 150 95191 0.43% 151 18736781 84.50% 22174722 reads passed initial QC criterion=sequence-density sequence-density=0.36 sequence-density-rank=1 fanout-score=28.70 fanout-score-rank=10 prefix-density=0.36 prefix-fanout=28.7 sequence=GGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGCTTGGTATCTCGTATGCCGTCTTCTGCTTG criterion=fanout-score sequence-density=0.02 sequence-density-rank=33 fanout-score=189.18 fanout-score-rank=1 prefix-density=0.23 prefix-fanout=17.5 sequence=AAGAAGAAGCTCCTGACTCGAGTTCTAGCAACCTCTCCTCGTCTACGTTTAGCAGAGGGAGAATATCGTACAGGACTAAAGGTAACACAACTGGAGGAGGTTATGCATACAAGAAAGCTAAGTGATTAATCA criterion=sequence-density sequence-density=0.27 sequence-density-rank=1 fanout-score=2.85 fanout-score-rank=32 prefix-density=0.28 prefix-fanout=2.7 sequence=ATGTACCCTGAC criterion=fanout-score sequence-density=0.10 sequence-density-rank=16 fanout-score=361.84 fanout-score-rank=1 prefix-density=1.04 prefix-fanout=33.8 sequence=AAGAAGAAGAAA SRR26075394 testing PE reads STAR mapping to Ensembl genome Started job on | Feb 12 00:46:35 Started mapping on | Feb 12 00:46:35 Finished on | Feb 12 00:51:18 Mapping speed, Million of reads per hour | 282.08 Number of input reads | 22174722 Average input read length | 294 UNIQUE READS: Uniquely mapped reads number | 19466949 Uniquely mapped reads % | 87.79% Average mapped length | 292.51 Number of splices: Total | 18920297 Number of splices: Annotated (sjdb) | 18470205 Number of splices: GT/AG | 18567681 Number of splices: GC/AG | 274576 Number of splices: AT/AC | 20463 Number of splices: Non-canonical | 57577 Mismatch rate per base, % | 0.42% Deletion rate per base | 0.03% Deletion average length | 3.37 Insertion rate per base | 0.02% Insertion average length | 2.61 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 580158 % of reads mapped to multiple loci | 2.62% Number of reads mapped to too many loci | 105508 % of reads mapped to too many loci | 0.48% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 8.49% % of reads unmapped: other | 0.63% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 2127615 2127615 2127615 N_multimapping 580158 580158 580158 N_noFeature 468995 19267523 571891 N_ambiguous 204505 966 107363 UnstrandedReadsAssigned:18793449 PositiveStrandReadsAssigned:198460 NegativeStrandReadsAssigned:18787695 Dataset is classified negative stranded MeadianReadLen=151 20thPercentileLength=151 echo kmer=147 SRR26075394 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,400 [index] number of k-mers: 62,057,036 [index] number of equivalence classes: 130,681 [quant] running in paired-end mode [quant] will process pair 1: SRR26075394-trimmed-pair1.fastq SRR26075394-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 22,174,722 reads, 19,364,088 reads pseudoaligned [quant] estimated average fragment length: 213.514 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,136 rounds 52401 SRR26075394.ke.tsv 34699 SRR26075394.se.tsv 87100 total ==> SRR26075394.ke.tsv <== target_id length eff_length est_counts tpm Potri.005G200100.1.v4.1 2018 1805.49 1811 48.1626 Potri.005G024800.1.v4.1 1035 822.486 943 55.0515 Potri.004G059700.1.v4.1 961 748.486 11 0.705659 Potri.007G009000.2.v4.1 1416 1203.49 0 0 Potri.003G141000.2.v4.1 2943 2730.49 850 14.9474 Potri.016G087400.1.v4.1 270 90.0848 1653.86 881.519 Potri.015G069301.1.v4.1 564 353.134 0 0 Potri.010G195200.1.v4.1 1773 1560.49 218 6.70783 Potri.012G127500.1.v4.1 977 764.486 9160 575.323 ==> SRR26075394.se.tsv <== Potri.001G166300.v4.1 0 Potri.001G448400.v4.1 309 Potri.001G233950.v4.1 0 Potri.001G122700.v4.1 212 Potri.001G212900.v4.1 0 Potri.001G182400.v4.1 0 Potri.001G256600.v4.1 0 Potri.001G040500.v4.1 0 Potri.001G416900.v4.1 2 Potri.001G452600.v4.1 771 SRR26075394 completed mapping pipeline successfully