Starting /dee2/code/volunteer_pipeline.sh SRR26075395 current disk space = 3051349147648 free memory = 1528282424 SRR26075395 SRAfilesize f061cf5987c2e6ac150a787d4fec332a SRR26075395.sra SRR26075395.sra file validated SRR26075395 is paired end SRR26075395 is conventional basespace SRR26075395 read1 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR26075395_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.4455 37.0 37.0 37.0 37.0 37.0 2 36.6115 37.0 37.0 37.0 37.0 37.0 3 36.638 37.0 37.0 37.0 37.0 37.0 4 36.6245 37.0 37.0 37.0 37.0 37.0 5 36.723 37.0 37.0 37.0 37.0 37.0 6 36.668 37.0 37.0 37.0 37.0 37.0 7 36.609 37.0 37.0 37.0 37.0 37.0 8 36.7165 37.0 37.0 37.0 37.0 37.0 9 36.6355 37.0 37.0 37.0 37.0 37.0 10-14 36.637299999999996 37.0 37.0 37.0 37.0 37.0 15-19 36.6169 37.0 37.0 37.0 37.0 37.0 20-24 36.5489 37.0 37.0 37.0 37.0 37.0 25-29 36.46169999999999 37.0 37.0 37.0 37.0 37.0 30-34 36.42249999999999 37.0 37.0 37.0 37.0 37.0 35-39 36.4149 37.0 37.0 37.0 37.0 37.0 40-44 36.3163 37.0 37.0 37.0 37.0 37.0 45-49 36.3156 37.0 37.0 37.0 37.0 37.0 50-54 36.2387 37.0 37.0 37.0 37.0 37.0 55-59 36.15650000000001 37.0 37.0 37.0 37.0 37.0 60-64 36.22429999999999 37.0 37.0 37.0 37.0 37.0 65-69 36.0406 37.0 37.0 37.0 37.0 37.0 70-74 36.0754 37.0 37.0 37.0 37.0 37.0 75-79 35.9625 37.0 37.0 37.0 37.0 37.0 80-84 35.952299999999994 37.0 37.0 37.0 37.0 37.0 85-89 35.845800000000004 37.0 37.0 37.0 37.0 37.0 90-94 35.7599 37.0 37.0 37.0 37.0 37.0 95-99 35.8158 37.0 37.0 37.0 37.0 37.0 100-104 35.6464 37.0 37.0 37.0 37.0 37.0 105-109 35.6435 37.0 37.0 37.0 37.0 37.0 110-114 35.5278 37.0 37.0 37.0 37.0 37.0 115-119 35.438599999999994 37.0 37.0 37.0 37.0 37.0 120-124 35.50450000000001 37.0 37.0 37.0 37.0 37.0 125-129 35.424099999999996 37.0 37.0 37.0 37.0 37.0 130-134 35.2318 37.0 37.0 37.0 32.2 37.0 135-139 35.1142 37.0 37.0 37.0 29.8 37.0 140-144 35.0732 37.0 37.0 37.0 27.4 37.0 145-149 35.0251 37.0 37.0 37.0 25.0 37.0 150-151 34.8495 37.0 37.0 37.0 25.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.0 1101 2 0.0 1101 3 0.0 1101 4 0.0 1101 5 0.0 1101 6 0.0 1101 7 0.0 1101 8 0.0 1101 9 0.0 1101 10-14 0.0 1101 15-19 0.0 1101 20-24 0.0 1101 25-29 0.0 1101 30-34 0.0 1101 35-39 0.0 1101 40-44 0.0 1101 45-49 0.0 1101 50-54 0.0 1101 55-59 0.0 1101 60-64 0.0 1101 65-69 0.0 1101 70-74 0.0 1101 75-79 0.0 1101 80-84 0.0 1101 85-89 0.0 1101 90-94 0.0 1101 95-99 0.0 1101 100-104 0.0 1101 105-109 0.0 1101 110-114 0.0 1101 115-119 0.0 1101 120-124 0.0 1101 125-129 0.0 1101 130-134 0.0 1101 135-139 0.0 1101 140-144 0.0 1101 145-149 0.0 1101 150-151 0.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 16 1.0 17 0.0 18 0.0 19 0.0 20 1.0 21 1.0 22 6.0 23 4.0 24 2.0 25 7.0 26 10.0 27 19.0 28 18.0 29 26.0 30 26.0 31 67.0 32 69.0 33 99.0 34 153.0 35 456.0 36 2836.0 37 199.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 37.85750125439037 15.905669844455595 7.325639739086803 38.91118916206723 2 18.175 13.450000000000001 36.449999999999996 31.924999999999997 3 16.375 17.45 29.825000000000003 36.35 4 22.35 22.25 27.200000000000003 28.199999999999996 5 23.849999999999998 30.525000000000002 24.5 21.125 6 20.625 35.475 21.725 22.175 7 17.075000000000003 26.375 39.75 16.8 8 17.65 25.025 33.575 23.75 9 18.099999999999998 22.75 34.949999999999996 24.2 10-14 18.915000000000003 29.975 27.685 23.425 15-19 18.9 28.23 28.110000000000003 24.759999999999998 20-24 20.52 27.51 27.779999999999998 24.19 25-29 19.46 27.584999999999997 28.79 24.165 30-34 19.73 27.839999999999996 28.54 23.89 35-39 19.21 28.26 28.389999999999997 24.14 40-44 20.11 28.15 27.715 24.025 45-49 19.62 27.63 27.83 24.92 50-54 19.325 27.534999999999997 28.255000000000003 24.884999999999998 55-59 19.715 27.07 28.83 24.385 60-64 20.665 25.905 28.044999999999998 25.385 65-69 19.72 27.595 28.799999999999997 23.885 70-74 20.745 27.200000000000003 27.975 24.08 75-79 19.99 27.145000000000003 27.834999999999997 25.03 80-84 20.105 27.450000000000003 28.73 23.715 85-89 20.645 26.76 28.71 23.885 90-94 20.59 27.389999999999997 27.334999999999997 24.685000000000002 95-99 20.44 27.565 28.134999999999998 23.86 100-104 19.905 27.465 28.365000000000002 24.265 105-109 19.605 26.915 28.475 25.005 110-114 20.165 27.115000000000002 27.694999999999997 25.025 115-119 20.979999999999997 26.790000000000003 27.41 24.82 120-124 20.69 27.58 27.55 24.18 125-129 21.135 26.784999999999997 27.505000000000003 24.575 130-134 20.775 27.755000000000003 27.37 24.099999999999998 135-139 21.845 27.255000000000003 26.43 24.47 140-144 21.224999999999998 27.169999999999998 27.73 23.875 145-149 20.64 27.275 26.815 25.27 150-151 20.4625 27.3 26.487500000000004 25.75 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.5 4 0.5 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.5 16 0.5 17 0.0 18 0.0 19 1.0 20 1.5 21 2.5 22 2.5 23 2.0 24 3.5 25 4.5 26 4.5 27 6.0 28 9.0 29 7.5 30 12.5 31 21.5 32 33.0 33 41.0 34 46.5 35 55.5 36 72.5 37 88.0 38 98.5 39 136.0 40 181.0 41 221.5 42 221.0 43 226.0 44 295.0 45 303.0 46 272.0 47 278.5 48 250.0 49 216.0 50 197.0 51 147.5 52 112.5 53 109.5 54 96.0 55 65.5 56 37.0 57 22.0 58 15.5 59 14.5 60 13.0 61 10.5 62 9.5 63 6.0 64 4.5 65 4.5 66 6.0 67 6.0 68 3.5 69 2.0 70 1.0 71 0.5 72 0.5 73 0.0 74 0.0 75 0.0 76 0.0 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.35000000000000003 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150-151 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 63.425 #Duplication Level Percentage of deduplicated Percentage of total 1 65.90461174615689 41.8 2 20.417816318486402 25.900000000000002 3 7.9621600315333065 15.15 4 3.1533307055577455 8.0 5 1.4189988175009853 4.5 6 0.8277493102089082 3.15 7 0.1970831690973591 0.8750000000000001 8 0.07883326763894363 0.4 9 0.039416633819471816 0.22499999999999998 >10 0.0 0.0 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTTACAACTTGCATTGGTTTTACAATATTACCTCCGGTTTTCACCCTTTG 9 0.22499999999999998 No Hit CTGAAATCTTGAATTTGACCCCTCGTCATACAGTAGCATATCTCGAATCA 8 0.2 No Hit CGCTACGTGATACATTTCTCCCAGGAAGCATCTGAGTAGCACTTACAGGC 8 0.2 No Hit CTTGATTGTCACCATCTTCTTCAGAAATGTTGAATAAGAGCTTTGGAAGA 7 0.17500000000000002 No Hit GCTCACCATCAAAGTTATTAGCCCTCAGAAGAAGAACACTCAATGATGAA 7 0.17500000000000002 No Hit ACCTAGGGACCTTTTGATAAGTCAAAGAAAGCAGGTGAATTGCATAGGCT 7 0.17500000000000002 No Hit GAATACTGGAAGACCAGTGTTCTGTTCTTGTTGTTGAATTCCGGTATCTT 7 0.17500000000000002 No Hit AGCGTCGCAGAGACAACAGCAACACAAGGCAGCACAACATCCCTTCCAGA 7 0.17500000000000002 No Hit CTGCACTTCACTTGAACTGCTAGTGTCTTGCTGATACCTGGACAATCATC 6 0.15 No Hit GTTCTGAGAATCCTCGTGAATACCAAGCTTCAGATTCTTAGAGAATGCCT 6 0.15 No Hit CCACACCTTCCATTTTTCCCAAAACCCTTTTCACAGCCCCAACACAACCT 6 0.15 No Hit GTAAATACTAGATTGATTCAAAAACCATGAATCTTGATCTGGTCCTTCTT 6 0.15 No Hit GTCTGATAACCGACATCCCACCCGGAGTTGTCAACCTAAAGATGCCATGG 6 0.15 No Hit GTGCTTCTTCACGAAGTTCTCATCGGCAAACTTAGCCCCATAGATTGATT 6 0.15 No Hit TGGCGCAATGAATTTACATGGAGGGCACCATGAAGCAGTAAAATCCACCA 6 0.15 No Hit GCCCACAACACTGCTACCACTAGAACAAATGTCAAAATACCAAACTCCGA 6 0.15 No Hit CCCGCGTTTTCCCCAATCTTCTCGAAACCAAAGATACACACGCATTCACA 6 0.15 No Hit GCAAGATACATCACTAGCAAGTGGATCCGCTACTCTCCACCACGACCAAA 6 0.15 No Hit GCATACTTAAAATAGTTCCGAGAACATTATAGATTCCCTTTAACAATTGC 6 0.15 No Hit GCATCTTCAATGAAAACTTGGAGATCTACACTCTTGAATCTGTTTTGATT 6 0.15 No Hit GATCGAGCAGATTGGTATAGTCATTAATGTAGTTGTCGATTTGTTCTCGT 6 0.15 No Hit GCCGAGATAGTACCTTGGAGTTGAATTTCCCACACTAAAGAAGCCTAGAG 6 0.15 No Hit GCTACAGTTGAATAAGAGAACACACAGTTTGCACCATAGTGAGTATGAGG 6 0.15 No Hit CTGAATTGGGCCCGACAATGGATTGGTCCCCTTTTCATCCATCTGAAGGA 6 0.15 No Hit ATCGGTAACTTCTTCATTAGCATCCATGCTGAGGACTACAGATTGGCTGT 6 0.15 No Hit TGGCAAACCAGTCTTGACTCCTAGTCCCAGATGGTCACAGATGACCTTGG 6 0.15 No Hit GAAGGAACGATATCTTCAAGCTTTTTCCCATTAAATATATCAATCCCCAC 6 0.15 No Hit GGCACAGTCACAGTAAGAACTCCATTTTCCATAGAAGCCTTGACCTGATC 6 0.15 No Hit CTTGACTTCCTCTTTAGGCACAGTGACAGTAAGAACCCCGTTCTCCATAG 6 0.15 No Hit ACATGGATCATCACACACCATCGCCACCATCTACCATCTATACATACATC 5 0.125 No Hit ATTTGATTCCACGGAATCCAGCTCCAGTGGCCAGGGCCATGAATTCATGT 5 0.125 No Hit CACCAAGTGGAGTGTGGATTCCTTCTGGATGTTGTAGTCAGCAAGGGTGC 5 0.125 No Hit CTGTGATGTGTTTTCCCAATGACTTTCATCTCATTCTGAAATTCTCTTTC 5 0.125 No Hit AGCAGCAGAAGAATCTAAGCAGTTTCCCATTTGGCAAACCAAAGCAAAGC 5 0.125 No Hit GTCTCTTTCCAATCAATGCGGGTGTTGACAAAGGCTGAATTTTCACGAGA 5 0.125 No Hit GCTGGATTTTCACGAGAAAAGAGAGAGTTTGAAGAGGAAATGAAAGGGGT 5 0.125 No Hit GCCGAAGTTATGCTTTAAACATTTAATAGACATTAAAGTTACAAATAAGA 5 0.125 No Hit GCCTTCCCCACATTCTCCGGCTTGCCAGCCCAGGCCTTGAGGGTACTCTG 5 0.125 No Hit ATCGTCTTCGAGCCCCCAACTTTCGTTCTTGATTAATGAAAACATCCTTG 5 0.125 No Hit GTACAAAGGGCAGGGACGTAGTCAACGCGAGCTGATGACTCGCGCTTACT 5 0.125 No Hit AGTGGACATAGTTTTATGCAGTAGATCCTGACGCTAGTAGAAGTGAAGGA 5 0.125 No Hit ATCCACTTCCCTCCCGACAATTTCAAGCACTCTTTGACTCTCTTTTCAAA 5 0.125 No Hit GTTCCTCATCATTTTTGACATCTAATGCTTGAGGTTGTTGCGCACACACC 5 0.125 No Hit ATCTTGAGTCACATAGGATATCCATTGCATTTGAGGGTGGATTCCTTTCT 5 0.125 No Hit CCATGGGCTCCCCAGCCACCATAGGTGTCAATAATGATCTTGCGTCCAGT 5 0.125 No Hit CCGCGGGATTGAGCTTCGTTCACGGTGATGTTACGGCCATCGAGGTCCTG 5 0.125 No Hit CTTCTTCTCTTGCTTTCTCTTGCCAGTCTGGATGCATAGATAGAACTACC 5 0.125 No Hit AGCCAACGTTGTCTTTCCACATCCTCCAGGAGCAGATAACACAATATGCT 5 0.125 No Hit CCAACAGATGTTATACTTGAATTTTTTTTGAAAACCCAAATATTGAAAAA 5 0.125 No Hit ATCCCTCAAACACTTCTCTACAGGCTCCATGCACTTCCTGAAGAGATCCA 5 0.125 No Hit GCACATGTCATACAACCAAACATAGGAAAGATAATAATAATAATAGATTT 5 0.125 No Hit TCACGGTGTTCCTCATGTTATAAGCATAATTTTCCAGAGCATTCTTGGCT 5 0.125 No Hit GCACATTTCACAGCCCCTTCTCAATTACTTTGGTGCTAAGAGCACTTCTA 5 0.125 No Hit CTCTGTCTTTGCACTCCTCAACCACTTTTTTTGTATCCTTCGAGTCTTTA 5 0.125 No Hit AGAAGAAGGGAAAGGGAAGTCCTTGAATGGATCGAAAGAAGAGAAAGGGT 5 0.125 No Hit GCAGGACATGGAAAGGATGAACCCTGACTCTCAAATGGAAAGCATCTTTT 5 0.125 No Hit GCCAAATCCGCGAAAACTGGCGCAATGAATTTACATGGAGGGCACCATGA 5 0.125 No Hit AGGTTCACAAAGATTTGACAAACAGAACTCCATATGAAGGCATGATGGCA 5 0.125 No Hit GGGAGGACAGGTTTTGGACGAAGAAAGAGATTTCCTCTATAGACTCAGCA 5 0.125 No Hit CAGGTATTGATAGCGCTGTCCAAAGAGAGAGCTGACAATTTGAAAGCCTC 5 0.125 No Hit CTCTTTTAGCCAAAACAATAAATTTCATTACGTCCAATATAAAATTTGAA 5 0.125 No Hit AAAACGGGAAAATAAGCTGGGTTTCTTCACAAGAGAGCCACTACCACTCG 5 0.125 No Hit ATCGTTTTTCCTCTATAGTTTCCCTTGAACCTCAACAATCTACGCTCAGG 5 0.125 No Hit CTTCCTTTTAACACCTCCAAACGTTGAAGAATCGCAAATAACCAAGTAGT 5 0.125 No Hit GCCTTGGTTTCTTCTATACAAGAAGATGAACAGTATATGGGAGTACCGAA 5 0.125 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.05 0.0 0.0 0.0 0.0 52-53 0.1 0.0 0.0 0.0 0.0 54-55 0.1 0.0 0.0 0.0 0.0 56-57 0.1 0.0 0.0 0.0 0.0 58-59 0.1 0.0 0.0 0.0 0.0 60-61 0.1 0.0 0.0 0.0 0.0 62-63 0.1 0.0 0.0 0.0 0.0 64-65 0.1 0.0 0.0 0.0 0.0 66-67 0.1 0.0 0.0 0.0 0.0 68-69 0.1 0.0 0.0 0.0 0.0 70-71 0.1125 0.0 0.0 0.0 0.0 72-73 0.125 0.0 0.0 0.0 0.0 74-75 0.125 0.0 0.0 0.0 0.0 76-77 0.125 0.0 0.0 0.0 0.0 78-79 0.125 0.0 0.0 0.0 0.0 80-81 0.125 0.0 0.0 0.0 0.0 82-83 0.1375 0.0 0.0 0.0 0.0 84-85 0.15 0.0 0.0 0.0 0.0 86-87 0.225 0.0 0.0 0.0 0.0 88-89 0.30000000000000004 0.0 0.0 0.0 0.0 90-91 0.4125 0.0 0.0 0.0 0.0 92-93 0.4875 0.0 0.0 0.0 0.0 94-95 0.6125 0.0 0.0 0.0 0.0 96-97 0.6875 0.0 0.0 0.0 0.0 98-99 0.95 0.0 0.0 0.0 0.0 100-101 1.075 0.0 0.0 0.0 0.0 102-103 1.35 0.0 0.0 0.0 0.0 104-105 1.5375 0.0 0.0 0.0 0.0 106-107 1.775 0.0 0.0 0.0 0.0 108-109 2.0375 0.0 0.0 0.0 0.0 110-111 2.4875 0.0 0.0 0.0 0.0 112-113 2.775 0.0 0.0 0.0 0.0 114-115 3.075 0.0 0.0 0.0 0.0 116-117 3.5625 0.0 0.0 0.0 0.0 118-119 4.0625 0.0 0.0 0.0 0.0 120-121 4.625 0.0 0.0 0.0 0.0 122-123 5.15 0.0 0.0 0.0 0.0 124-125 5.8125 0.0 0.0 0.0 0.0 126-127 6.35 0.0 0.0 0.0 0.0 128-129 6.925 0.0 0.0 0.0 0.0 130-131 7.6125 0.0 0.0 0.0 0.0 132-133 8.25 0.0 0.0 0.0 0.0 134-135 9.1875 0.0 0.0 0.0 0.0 136-137 9.9125 0.0 0.0 0.0 0.0 138-139 10.8875 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TTTTGTG 10 0.006830828 145.0 145 GCAAGAT 10 0.006830828 145.0 1 TGTTAAC 10 0.006830828 145.0 6 TAACAGG 10 0.006830828 145.0 9 TTAACAG 10 0.006830828 145.0 8 GACTTCT 10 0.006830828 145.0 3 GTTAACA 10 0.006830828 145.0 7 GGGGGGG 35 0.0035366106 20.714287 140-144 >>END_MODULE SRR26075395 read2 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR26075395_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.2925 37.0 37.0 37.0 37.0 37.0 2 36.2685 37.0 37.0 37.0 37.0 37.0 3 36.2745 37.0 37.0 37.0 37.0 37.0 4 36.3425 37.0 37.0 37.0 37.0 37.0 5 36.416 37.0 37.0 37.0 37.0 37.0 6 36.2715 37.0 37.0 37.0 37.0 37.0 7 36.314 37.0 37.0 37.0 37.0 37.0 8 36.455 37.0 37.0 37.0 37.0 37.0 9 36.453 37.0 37.0 37.0 37.0 37.0 10-14 36.304500000000004 37.0 37.0 37.0 37.0 37.0 15-19 36.3269 37.0 37.0 37.0 37.0 37.0 20-24 36.311899999999994 37.0 37.0 37.0 37.0 37.0 25-29 36.2024 37.0 37.0 37.0 37.0 37.0 30-34 36.14880000000001 37.0 37.0 37.0 37.0 37.0 35-39 36.1682 37.0 37.0 37.0 37.0 37.0 40-44 36.1109 37.0 37.0 37.0 37.0 37.0 45-49 36.0832 37.0 37.0 37.0 37.0 37.0 50-54 35.99380000000001 37.0 37.0 37.0 37.0 37.0 55-59 36.04350000000001 37.0 37.0 37.0 37.0 37.0 60-64 36.0242 37.0 37.0 37.0 37.0 37.0 65-69 35.98310000000001 37.0 37.0 37.0 37.0 37.0 70-74 35.869 37.0 37.0 37.0 37.0 37.0 75-79 35.775200000000005 37.0 37.0 37.0 37.0 37.0 80-84 35.8014 37.0 37.0 37.0 37.0 37.0 85-89 35.8033 37.0 37.0 37.0 37.0 37.0 90-94 35.6671 37.0 37.0 37.0 37.0 37.0 95-99 35.801300000000005 37.0 37.0 37.0 37.0 37.0 100-104 35.663700000000006 37.0 37.0 37.0 37.0 37.0 105-109 35.6006 37.0 37.0 37.0 37.0 37.0 110-114 35.5659 37.0 37.0 37.0 37.0 37.0 115-119 35.5114 37.0 37.0 37.0 37.0 37.0 120-124 35.4314 37.0 37.0 37.0 37.0 37.0 125-129 35.3818 37.0 37.0 37.0 37.0 37.0 130-134 35.3803 37.0 37.0 37.0 37.0 37.0 135-139 35.2774 37.0 37.0 37.0 32.2 37.0 140-144 35.1467 37.0 37.0 37.0 27.4 37.0 145-149 35.1401 37.0 37.0 37.0 25.0 37.0 150-151 34.914 37.0 37.0 37.0 25.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.0 1101 2 0.0 1101 3 0.0 1101 4 0.0 1101 5 0.0 1101 6 0.0 1101 7 0.0 1101 8 0.0 1101 9 0.0 1101 10-14 0.0 1101 15-19 0.0 1101 20-24 0.0 1101 25-29 0.0 1101 30-34 0.0 1101 35-39 0.0 1101 40-44 0.0 1101 45-49 0.0 1101 50-54 0.0 1101 55-59 0.0 1101 60-64 0.0 1101 65-69 0.0 1101 70-74 0.0 1101 75-79 0.0 1101 80-84 0.0 1101 85-89 0.0 1101 90-94 0.0 1101 95-99 0.0 1101 100-104 0.0 1101 105-109 0.0 1101 110-114 0.0 1101 115-119 0.0 1101 120-124 0.0 1101 125-129 0.0 1101 130-134 0.0 1101 135-139 0.0 1101 140-144 0.0 1101 145-149 0.0 1101 150-151 0.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 13 4.0 14 8.0 15 2.0 16 5.0 17 0.0 18 2.0 19 2.0 20 4.0 21 3.0 22 9.0 23 8.0 24 4.0 25 8.0 26 9.0 27 16.0 28 15.0 29 18.0 30 30.0 31 23.0 32 34.0 33 71.0 34 180.0 35 626.0 36 2719.0 37 200.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.225 25.45 9.4 21.925 2 29.599999999999998 28.575 25.224999999999998 16.6 3 22.0 29.849999999999998 29.9 18.25 4 26.974999999999998 33.375 22.325 17.325 5 25.05 37.2 19.225 18.525 6 21.075 39.324999999999996 21.85 17.75 7 23.200000000000003 24.05 33.35 19.400000000000002 8 22.675 28.249999999999996 25.724999999999998 23.35 9 23.375 26.275 28.575 21.775 10-14 25.35 30.415 23.94 20.294999999999998 15-19 24.5 28.549999999999997 26.39 20.560000000000002 20-24 23.855 29.145 26.435 20.565 25-29 25.14 29.145 25.845000000000002 19.869999999999997 30-34 23.815 28.884999999999998 26.669999999999998 20.630000000000003 35-39 24.91 28.15 26.755000000000003 20.185 40-44 24.635 28.675 26.035000000000004 20.655 45-49 25.055 28.525 27.105 19.314999999999998 50-54 24.515 28.93 26.68 19.875 55-59 24.32 28.715000000000003 26.424999999999997 20.54 60-64 25.405 27.750000000000004 26.415 20.43 65-69 25.040000000000003 29.465000000000003 26.435 19.06 70-74 25.115 28.599999999999998 26.195 20.09 75-79 23.985 29.365000000000002 26.68 19.97 80-84 24.555 28.660000000000004 26.634999999999998 20.150000000000002 85-89 25.155 28.22 26.729999999999997 19.895 90-94 24.88 28.125 26.419999999999998 20.575 95-99 24.43 28.585 27.075 19.91 100-104 25.025 29.09 26.085 19.8 105-109 24.67 28.555000000000003 26.724999999999998 20.05 110-114 25.330000000000002 28.275 26.640000000000004 19.755 115-119 24.13 29.520000000000003 26.665 19.685 120-124 25.34 27.994999999999997 26.290000000000003 20.375 125-129 26.1 28.435 26.135 19.33 130-134 25.94 28.415000000000003 26.55 19.095000000000002 135-139 25.955000000000002 27.685 26.665 19.695 140-144 27.060000000000002 28.849999999999998 25.735000000000003 18.355 145-149 27.48 27.91 26.305 18.305 150-151 28.0625 28.212500000000002 25.4375 18.2875 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.5 6 1.0 7 0.5 8 0.5 9 0.5 10 0.5 11 1.0 12 1.0 13 0.5 14 0.5 15 2.0 16 2.5 17 1.5 18 1.0 19 1.0 20 0.5 21 0.5 22 1.0 23 0.5 24 0.5 25 1.0 26 2.0 27 1.5 28 2.5 29 7.5 30 10.5 31 9.5 32 10.5 33 19.0 34 33.0 35 60.0 36 79.0 37 82.0 38 99.0 39 138.0 40 197.0 41 218.0 42 241.0 43 274.5 44 278.5 45 291.0 46 286.0 47 283.5 48 261.5 49 213.5 50 189.0 51 176.0 52 134.5 53 82.5 54 61.0 55 53.5 56 49.0 57 28.5 58 13.0 59 19.5 60 15.5 61 8.5 62 7.0 63 7.0 64 7.0 65 4.0 66 2.0 67 1.0 68 0.5 69 1.0 70 1.0 71 0.5 72 0.5 73 0.5 74 1.5 75 1.5 76 1.5 77 1.5 78 1.0 79 0.5 80 0.0 81 0.0 82 0.0 83 0.5 84 0.5 85 0.0 86 0.0 87 0.0 88 0.0 89 0.5 90 0.5 91 0.5 92 1.0 93 1.0 94 0.5 95 0.5 96 1.0 97 0.5 98 0.0 99 1.0 100 3.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150-151 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 63.425 #Duplication Level Percentage of deduplicated Percentage of total 1 66.25936145053213 42.025 2 20.299566417027986 25.75 3 7.686243594797004 14.625 4 3.1139140717382734 7.9 5 1.3401655498620417 4.25 6 0.7883326763894364 3.0 7 0.27591643673630273 1.225 8 0.1970831690973591 1.0 9 0.039416633819471816 0.22499999999999998 >10 0.0 0.0 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source TCTGAAAATCGTAAATGCAAGCTGGTGTCCAAATTTGGTGTCTTTCCCCA 9 0.22499999999999998 No Hit GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG 8 0.2 No Hit GATCCCTATTTCTTTGTACATTTCCATGGAGCTTGTCCGGGTTGGTCAGG 8 0.2 No Hit GAGGCAAAGAAGATGAGAAGAGAAGTAGCTGTAATGGAGAAGGAGGTTGC 8 0.2 No Hit ACTGCACCAATGAGTCGCTGCATGAGAATAAATTTAGAGGTGATATCAGA 8 0.2 No Hit AATCAACACCAAACTCGAAGTCTTCTCTCTGTCTTCTCGATTCCTTGTTT 8 0.2 No Hit TAGATGCAAATCTTTGCCTTCTTCGCCTCTATCAGTTTGAGCCGGAGAGA 7 0.17500000000000002 No Hit CATGTCTTCAAGGCAGATCTTCCAGGGCTTAAAAAAGAGGAAGTGAAGGT 7 0.17500000000000002 No Hit CAGCAATTCACAACGATTATTCTTCAGCAGACGACCAACCAGCAAAGCAA 7 0.17500000000000002 No Hit ATTTTTGAAGAGAGATTTGAAGATGGTTGGGAGAGCCGCTGGGTTAGATC 7 0.17500000000000002 No Hit TGGATTTGAGTAACAATCAATTTTCAGGCATGCTTCCAAGAAGCTTTTTT 7 0.17500000000000002 No Hit CTTTATCTTATTATCATGTGACAGGGTGGTTTCTTCTTGATTTCTCTCAC 7 0.17500000000000002 No Hit CCACAACCGAACTTCTCTCGTCCTTATCTCCATTCTTCCATAGCTCTCAT 7 0.17500000000000002 No Hit CCTCAGATCACTGTCTGCTTTGATATTGATGCAAACGGTATCTTGAATGT 6 0.15 No Hit GTGACACTGGACAACAACTATAACACATACAGTTTTAATGGCAAGAAGAA 6 0.15 No Hit GGGGCATAGTGCAGGCCGTTAGGCATTATAATGATCCTCATGTTTTGGTT 6 0.15 No Hit GTTTGCAAGCTTCGGTGATCCTTTGGGTACCTGTGGAGACTATGCTGTTG 6 0.15 No Hit CTAGTGCCCATGAATCTAATTCACAGCCTTACCATGGCCAGAAGATGCAT 6 0.15 No Hit CATCAAGAACCGTCCTTGCAAGGTTGTTGAGGTTTCCACCTCAAAGACAG 6 0.15 No Hit CGAGAATTTCCGTGCTCTTTGCACCGGTGAGAAAGGTGTTGGCAAGTCCG 6 0.15 No Hit GGAGAGAGGAATGTGGAGAAGGAAGACCAGAACGATACATGGCATCGTGT 6 0.15 No Hit GCTTACACTGCGGTATCGATGTGAGAGGACCGATGTTAATCGGTAGCAGA 6 0.15 No Hit ATGTTTGAAAAATATTTTAACTATTTTCCTTATACAATTCGTGGGTATGC 6 0.15 No Hit AAAGAGAGCAGCATACATCCATAGAGAGAAAGAGAAGACATGGCAACCAG 6 0.15 No Hit AATGGTACAGTGGTGCAGGACCTTGAATTAGGCCAGGTTATTCAACTTCA 6 0.15 No Hit AGAAGAAGCAGAACAACATCAAGCTCTATGTCCGCCGTGTCTTCATCATG 6 0.15 No Hit GCAAGAACGCAAAGAAAAGTTACAAACAAAACCAAGAGAGCAATGGATTG 6 0.15 No Hit CTTTCTTTCAGCCTCACAAATAGATAATGGGGTTCTATGTTTTGCTGCTT 6 0.15 No Hit TTCAATGATCAATTTCCTTGTCATTACTCCTAAGGATGTGGAAATCCTTG 6 0.15 No Hit GATAATGTTGCCAGAAGCCGTTGCCATTGTCATGGCACCAAGAGATTCTT 6 0.15 No Hit CAATATCCAGAAGGAGTCCACTCTGCACCTCGTGCTTCGCCTCCGTGGTG 6 0.15 No Hit GGCGCCGGAGCTTGAAGTAGCAATTGTGAAAGCAACGAGCCACGACGATG 6 0.15 No Hit CCGGAAGCCGGGTTACGGTGCCCAACTGCGCGCTAACCTAGAACCCACAA 6 0.15 No Hit GATATTCCATCGACTGGCGGCAGTGGTGCTGGCCCTAAGATTGAAGAAGT 5 0.125 No Hit AAAAGATTGTATCCGACAAAGAAAGAAGAGAGAGATGGCGTTGATTCCAA 5 0.125 No Hit AACCAGAAATGTCATGTCAAGAACAAGGATATCCGAAAATTTCTTGATGG 5 0.125 No Hit GACAAGCCCTGCTGCGAGAGCGATCTCTCAACTTGATGTGCTGATGATGA 5 0.125 No Hit AGCAAACGAGTGATTTTGTGAAGAAGAAGAAGAAGGATGGCAATGATTCC 5 0.125 No Hit GCAGAGTTCATTGGGCTATCTGTTTGGGAGTGGAGAGGCTCCAAAACCTG 5 0.125 No Hit CAGAGCTCTTAGGAGGTTGAGAACTGCCTGTGAGAGGGCAAAAAGGACTC 5 0.125 No Hit GGTGAACGGAGGAGAAGAGAAAGAGGATACGATCGACGCCACCGAGATAG 5 0.125 No Hit GGTCGTGCCTCCGGTGCTGTTACTTTGAAGAAATTAGAGTGCTCAAAGCA 5 0.125 No Hit CCACAACAAAGGAAGCTGGAGACATGTGTTCTACAAGGTCAGGTACGAGG 5 0.125 No Hit AGGATGGAGCAGAACTCCATGCACTCCCTTGCAACCATCATTTCCATGCA 5 0.125 No Hit GAGGAGTTTGCTCCTCGAGCTTTCACTTATGCAGAGCTAGAAAATGTTAC 5 0.125 No Hit TAAAGGAGTATGTCATCAAGCCTGTTATCCCGGAGAAGTACCTTGATGAG 5 0.125 No Hit TGATCACCATGCCATACTTGAGGGTACTCTCTTGAAGCCGAACATGGTTA 5 0.125 No Hit GGATCCTGTTGGGTTGAAGATTCCATTGAGTGATCTGGAGATAAAGCTGC 5 0.125 No Hit AAAAATATTTGGTTGCTTCAGTTTCGGTAAGAGAATGGCAGAAGAAGGAC 5 0.125 No Hit CCGTTAACGAACGAGACCTCAGCCTGCTAACTAGCTATGCGGAGGTGACC 5 0.125 No Hit CTCGCTTGGGCCACCACTGACCAAGTCCTCCAAGAGGCTTTTAGCCAGTA 5 0.125 No Hit CCATTCCAAAGGGCACCCTGTTCCCCATGTGTGGTATGAATTTGTGTTTC 5 0.125 No Hit CAGTCTTTGCTTATCCCTCATGAATTCCTATGTCCAATTACTCTGGAGAT 5 0.125 No Hit GCAATAAAACACTACAACCCCAGCCCCGCAGCGTATGACCAGTTTTTGAA 5 0.125 No Hit TGAAGAGAAGACCAAGAGGAAAGTCGAGATTATCTCTCCCCAACCTAAGA 5 0.125 No Hit CGAGGGAGAAGAGTCTGAGATTTGCAGAATAAGATATTTTCTTTAGAAAT 5 0.125 No Hit GAAGAATCTGAGGAAGAAACAGAAAAGGACAAGGGAGTTAAAGGTGGGGA 5 0.125 No Hit CAGATCTAGCAAACCAGCCACCCCCTTCCTGGCTCCCTCACCACACACGC 5 0.125 No Hit CTGATAATGACATGAAAATTGAAGATGTAATCGAGGAATGCAAGTTGTTC 5 0.125 No Hit GGCATTGCAAGACCGGGTTATGGAGGAAACAAAAGAAAAGAAAAATGCTG 5 0.125 No Hit GCTGATGCTGGTGAAAAGAAAGATGAGGCCAAAGTTATTTCCGTTTACAA 5 0.125 No Hit GCACTAGATGTGGCGGACTTCTTGTAACAAACCACAGAAACAGCAGCCCA 5 0.125 No Hit CAGTCACTGTTTACATGGAACACAAAGCAGCTTTTCATATTTGTAGCAGC 5 0.125 No Hit AGGTCAGGCGGGACTACCCGCTGAGTTTAAGCATATCAATAAGCGGAGGA 5 0.125 No Hit GTGTCCACAACCTTTAAAGGAAGTTCTCAAGTTGTGTGAGAATCGACGAG 5 0.125 No Hit GTGATTCCAAGTTCGGAAGAAGAACCTTTTCGTCAGGAACAACCATTTCC 5 0.125 No Hit AGAAAATAGTCCTGAAGTGAAAGGAACTGAAGCACCGGCACATGAAATAA 5 0.125 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.05 0.0 0.0 0.0 0.0 52-53 0.1 0.0 0.0 0.0 0.0 54-55 0.1 0.0 0.0 0.0 0.0 56-57 0.1 0.0 0.0 0.0 0.0 58-59 0.1 0.0 0.0 0.0 0.0 60-61 0.1 0.0 0.0 0.0 0.0 62-63 0.1 0.0 0.0 0.0 0.0 64-65 0.1 0.0 0.0 0.0 0.0 66-67 0.1 0.0 0.0 0.0 0.0 68-69 0.1 0.0 0.0 0.0 0.0 70-71 0.1125 0.0 0.0 0.0 0.0 72-73 0.125 0.0 0.0 0.0 0.0 74-75 0.125 0.0 0.0 0.0 0.0 76-77 0.125 0.0 0.0 0.0 0.0 78-79 0.125 0.0 0.0 0.0 0.0 80-81 0.125 0.0 0.0 0.0 0.0 82-83 0.1375 0.0 0.0 0.0 0.0 84-85 0.15 0.0 0.0 0.0 0.0 86-87 0.225 0.0 0.0 0.0 0.0 88-89 0.30000000000000004 0.0 0.0 0.0 0.0 90-91 0.4125 0.0 0.0 0.0 0.0 92-93 0.4875 0.0 0.0 0.0 0.0 94-95 0.6125 0.0 0.0 0.0 0.0 96-97 0.675 0.0 0.0 0.0 0.0 98-99 0.9375 0.0 0.0 0.0 0.0 100-101 1.075 0.0 0.0 0.0 0.0 102-103 1.35 0.0 0.0 0.0 0.0 104-105 1.5375 0.0 0.0 0.0 0.0 106-107 1.775 0.0 0.0 0.0 0.0 108-109 2.0999999999999996 0.0 0.0 0.0 0.0 110-111 2.5625 0.0 0.0 0.0 0.0 112-113 2.875 0.0 0.0 0.0 0.0 114-115 3.15 0.0 0.0 0.0 0.0 116-117 3.6375 0.0 0.0 0.0 0.0 118-119 4.1375 0.0 0.0 0.0 0.0 120-121 4.725 0.0 0.0 0.0 0.0 122-123 5.275 0.0 0.0 0.0 0.0 124-125 5.9375 0.0 0.0 0.0 0.0 126-127 6.4875 0.0 0.0 0.0 0.0 128-129 7.075 0.0 0.0 0.0 0.0 130-131 7.7625 0.0 0.0 0.0 0.0 132-133 8.45 0.0 0.0 0.0 0.0 134-135 9.45 0.0 0.0 0.0 0.0 136-137 10.1875 0.0 0.0 0.0 0.0 138-139 11.2 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTTGAAT 10 0.006830828 145.0 1 GGGCATA 10 0.006830828 145.0 2 GTGCAGG 10 0.006830828 145.0 9 GGCATAG 10 0.006830828 145.0 3 TAGTGCA 10 0.006830828 145.0 7 GAGCAAT 10 0.006830828 145.0 4 GCTGCTA 10 0.006830828 145.0 145 ATAGTGC 10 0.006830828 145.0 6 AGTGCAG 10 0.006830828 145.0 8 TTAAGGC 10 0.006830828 145.0 5 CATAGTG 10 0.006830828 145.0 5 GGGGCAT 10 0.006830828 145.0 1 TGAGCAA 10 0.006830828 145.0 3 CAAGTTA 10 0.006830828 145.0 1 GCATAGT 10 0.006830828 145.0 4 TAAGGCT 10 0.006830828 145.0 6 GTTAAGG 10 0.006830828 145.0 4 >>END_MODULE Read 2227818 spots for SRR26075395.sra Written 2227818 spots for SRR26075395.sra Read 2227818 spots for SRR26075395.sra Written 2227818 spots for SRR26075395.sra Read 2227818 spots for SRR26075395.sra Written 2227818 spots for SRR26075395.sra Read 2227818 spots for SRR26075395.sra Written 2227818 spots for SRR26075395.sra Read 2227818 spots for SRR26075395.sra Written 2227818 spots for SRR26075395.sra Read 2227818 spots for SRR26075395.sra Written 2227818 spots for SRR26075395.sra Read 2227818 spots for SRR26075395.sra Written 2227818 spots for SRR26075395.sra Read 2227818 spots for SRR26075395.sra Written 2227818 spots for SRR26075395.sra Read 2227818 spots for SRR26075395.sra Written 2227818 spots for SRR26075395.sra Read 2227818 spots for SRR26075395.sra Written 2227818 spots for SRR26075395.sra Read 2227818 spots for SRR26075395.sra Written 2227818 spots for SRR26075395.sra Read 2227822 spots for SRR26075395.sra Written 2227822 spots for SRR26075395.sra Read 2227818 spots for SRR26075395.sra Written 2227818 spots for SRR26075395.sra Read 2227818 spots for SRR26075395.sra Written 2227818 spots for SRR26075395.sra Read 2227818 spots for SRR26075395.sra Written 2227818 spots for SRR26075395.sra Read 2227818 spots for SRR26075395.sra Written 2227818 spots for SRR26075395.sra Read 2227818 spots for SRR26075395.sra Written 2227818 spots for SRR26075395.sra Read 2227818 spots for SRR26075395.sra Written 2227818 spots for SRR26075395.sra Read 2227818 spots for SRR26075395.sra Written 2227818 spots for SRR26075395.sra Read 2227818 spots for SRR26075395.sra Written 2227818 spots for SRR26075395.sra SRR ids: ['SRR26075395.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_lv97hicw SRR26075395.sra spots: 44556364 blocks: [[1, 2227818], [2227819, 4455636], [4455637, 6683454], [6683455, 8911272], [8911273, 11139090], [11139091, 13366908], [13366909, 15594726], [15594727, 17822544], [17822545, 20050362], [20050363, 22278180], [22278181, 24505998], [24505999, 26733816], [26733817, 28961634], [28961635, 31189452], [31189453, 33417270], [33417271, 35645088], [35645089, 37872906], [37872907, 40100724], [40100725, 42328542], [42328543, 44556364]] SRR26075395 file size 16456962 SRR26075395 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o SRR26075395 SRR26075395_1.fastq SRR26075395_2.fastq Input file: SRR26075395_1.fastq Paired file: SRR26075395_2.fastq trimmed: SRR26075395-trimmed-pair1.fastq, SRR26075395-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Wed Feb 12 00:45:46 2025 >> started Wed Feb 12 00:46:38 2025 >> done (52.257s) 44556364 read pairs processed; of these: 173 ( 0.00%) short read pairs filtered out after trimming by size control 34048 ( 0.08%) empty read pairs filtered out after trimming by size control 44522143 (99.92%) read pairs available; of these: 6633754 (14.90%) trimmed read pairs available after processing 37888389 (85.10%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 18 19 0.00% 19 24 0.00% 20 19 0.00% 21 30 0.00% 22 27 0.00% 23 40 0.00% 24 35 0.00% 25 32 0.00% 26 33 0.00% 27 39 0.00% 28 50 0.00% 29 47 0.00% 30 59 0.00% 31 71 0.00% 32 67 0.00% 33 60 0.00% 34 54 0.00% 35 63 0.00% 36 58 0.00% 37 66 0.00% 38 78 0.00% 39 62 0.00% 40 88 0.00% 41 81 0.00% 42 114 0.00% 43 92 0.00% 44 107 0.00% 45 151 0.00% 46 144 0.00% 47 124 0.00% 48 129 0.00% 49 150 0.00% 50 192 0.00% 51 214 0.00% 52 211 0.00% 53 250 0.00% 54 233 0.00% 55 271 0.00% 56 347 0.00% 57 324 0.00% 58 381 0.00% 59 434 0.00% 60 531 0.00% 61 571 0.00% 62 637 0.00% 63 713 0.00% 64 883 0.00% 65 879 0.00% 66 1166 0.00% 67 1179 0.00% 68 1281 0.00% 69 1598 0.00% 70 1690 0.00% 71 1954 0.00% 72 2437 0.01% 73 2858 0.01% 74 3271 0.01% 75 3584 0.01% 76 4038 0.01% 77 4642 0.01% 78 4968 0.01% 79 5783 0.01% 80 6450 0.01% 81 7465 0.02% 82 8554 0.02% 83 9390 0.02% 84 10953 0.02% 85 12436 0.03% 86 13409 0.03% 87 14678 0.03% 88 15729 0.04% 89 17724 0.04% 90 18961 0.04% 91 20982 0.05% 92 22680 0.05% 93 25486 0.06% 94 27779 0.06% 95 30432 0.07% 96 32774 0.07% 97 35337 0.08% 98 36932 0.08% 99 39137 0.09% 100 41558 0.09% 101 43847 0.10% 102 46924 0.11% 103 50416 0.11% 104 53027 0.12% 105 57397 0.13% 106 61591 0.14% 107 63094 0.14% 108 67067 0.15% 109 69002 0.15% 110 69784 0.16% 111 73800 0.17% 112 76332 0.17% 113 79622 0.18% 114 84066 0.19% 115 88213 0.20% 116 91535 0.21% 117 96710 0.22% 118 100068 0.22% 119 100982 0.23% 120 105948 0.24% 121 106650 0.24% 122 110072 0.25% 123 113585 0.26% 124 118774 0.27% 125 120228 0.27% 126 125274 0.28% 127 130529 0.29% 128 132593 0.30% 129 136373 0.31% 130 139532 0.31% 131 140680 0.32% 132 143964 0.32% 133 147609 0.33% 134 150605 0.34% 135 153921 0.35% 136 158097 0.36% 137 160776 0.36% 138 165791 0.37% 139 169202 0.38% 140 169610 0.38% 141 172960 0.39% 142 176660 0.40% 143 177354 0.40% 144 180827 0.41% 145 183793 0.41% 146 186149 0.42% 147 191362 0.43% 148 195064 0.44% 149 197299 0.44% 150 200417 0.45% 151 37888389 85.10% 44522143 reads passed initial QC criterion=sequence-density sequence-density=0.83 sequence-density-rank=1 fanout-score=3.41 fanout-score-rank=20 prefix-density=0.89 prefix-fanout=3.2 sequence=GCATTCTCAGGCAGCCT criterion=fanout-score sequence-density=0.17 sequence-density-rank=31 fanout-score=37.44 fanout-score-rank=1 prefix-density=1.09 prefix-fanout=5.7 sequence=TCATCTTCAATTTCGACCTTCACTTCCTCTTTTTTAAGCCCTGGAAGATC criterion=sequence-density sequence-density=0.46 sequence-density-rank=1 fanout-score=5.56 fanout-score-rank=13 prefix-density=0.66 prefix-fanout=3.9 sequence=GATGGCAATGAT criterion=fanout-score sequence-density=0.02 sequence-density-rank=40 fanout-score=404.89 fanout-score-rank=1 prefix-density=0.51 prefix-fanout=14.6 sequence=AAGAGAAGAAACACTAAAAAAATCACACAAGCGAAATCTCCAGCGAGAAGAGAAAGATGGATTTCAGAATCATGGGTCTTGACGCGCCACTCTTCAACACCCTCCAGCACATGATGGATGCAAGTGATCATGAGGCAGACAAGTCCTTCAATGCGCCAACACGCACTTACGTACGTGATGCCAAGGCAATGGCATCAACACCAGCTGATGTGAAAGAGTATCCAA SRR26075395 testing PE reads STAR mapping to Ensembl genome Started job on | Feb 12 00:47:25 Started mapping on | Feb 12 00:47:25 Finished on | Feb 12 00:53:30 Mapping speed, Million of reads per hour | 439.12 Number of input reads | 44522143 Average input read length | 295 UNIQUE READS: Uniquely mapped reads number | 40062681 Uniquely mapped reads % | 89.98% Average mapped length | 293.49 Number of splices: Total | 29772942 Number of splices: Annotated (sjdb) | 28864528 Number of splices: GT/AG | 29319165 Number of splices: GC/AG | 326224 Number of splices: AT/AC | 36303 Number of splices: Non-canonical | 91250 Mismatch rate per base, % | 0.42% Deletion rate per base | 0.03% Deletion average length | 2.79 Insertion rate per base | 0.03% Insertion average length | 3.23 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 1443183 % of reads mapped to multiple loci | 3.24% Number of reads mapped to too many loci | 336541 % of reads mapped to too many loci | 0.76% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 5.73% % of reads unmapped: other | 0.29% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 3016279 3016279 3016279 N_multimapping 1443183 1443183 1443183 N_noFeature 974001 39554031 1296468 N_ambiguous 439496 4147 250502 UnstrandedReadsAssigned:38649184 PositiveStrandReadsAssigned:504503 NegativeStrandReadsAssigned:38515711 Dataset is classified negative stranded MeadianReadLen=151 20thPercentileLength=151 echo kmer=147 SRR26075395 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,400 [index] number of k-mers: 62,057,036 [index] number of equivalence classes: 130,681 [quant] running in paired-end mode [quant] will process pair 1: SRR26075395-trimmed-pair1.fastq SRR26075395-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 44,522,143 reads, 39,327,232 reads pseudoaligned [quant] estimated average fragment length: 213.946 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,207 rounds 52401 SRR26075395.ke.tsv 34699 SRR26075395.se.tsv 87100 total ==> SRR26075395.ke.tsv <== target_id length eff_length est_counts tpm Potri.005G200100.1.v4.1 2018 1805.05 3566 41.457 Potri.005G024800.1.v4.1 1035 822.054 15291 390.339 Potri.004G059700.1.v4.1 961 748.054 167 4.68479 Potri.007G009000.2.v4.1 1416 1203.05 0 0 Potri.003G141000.2.v4.1 2943 2730.05 1177 9.04715 Potri.016G087400.1.v4.1 270 88.7223 3998 945.62 Potri.015G069301.1.v4.1 564 352.939 0 0 Potri.010G195200.1.v4.1 1773 1560.05 20 0.269028 Potri.012G127500.1.v4.1 977 764.054 36123 992.125 ==> SRR26075395.se.tsv <== Potri.001G166300.v4.1 0 Potri.001G448400.v4.1 366 Potri.001G233950.v4.1 0 Potri.001G122700.v4.1 260 Potri.001G212900.v4.1 4 Potri.001G182400.v4.1 0 Potri.001G256600.v4.1 0 Potri.001G040500.v4.1 1 Potri.001G416900.v4.1 0 Potri.001G452600.v4.1 232 SRR26075395 completed mapping pipeline successfully