Starting /dee2/code/volunteer_pipeline.sh SRR26075396 current disk space = 3051359494144 free memory = 1403680196 SRR26075396 SRAfilesize 0b7bcfffce99af0d69cc0cbc7f2351e6 SRR26075396.sra SRR26075396.sra file validated SRR26075396 is paired end SRR26075396 is conventional basespace SRR26075396 read1 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR26075396_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.64075 37.0 37.0 37.0 37.0 37.0 2 36.677 37.0 37.0 37.0 37.0 37.0 3 36.6695 37.0 37.0 37.0 37.0 37.0 4 36.765 37.0 37.0 37.0 37.0 37.0 5 36.7405 37.0 37.0 37.0 37.0 37.0 6 36.714 37.0 37.0 37.0 37.0 37.0 7 36.677 37.0 37.0 37.0 37.0 37.0 8 36.7265 37.0 37.0 37.0 37.0 37.0 9 36.7045 37.0 37.0 37.0 37.0 37.0 10-14 36.6506 37.0 37.0 37.0 37.0 37.0 15-19 36.6003 37.0 37.0 37.0 37.0 37.0 20-24 36.539 37.0 37.0 37.0 37.0 37.0 25-29 36.479 37.0 37.0 37.0 37.0 37.0 30-34 36.3474 37.0 37.0 37.0 37.0 37.0 35-39 36.404500000000006 37.0 37.0 37.0 37.0 37.0 40-44 36.2569 37.0 37.0 37.0 37.0 37.0 45-49 36.1899 37.0 37.0 37.0 37.0 37.0 50-54 36.1212 37.0 37.0 37.0 37.0 37.0 55-59 36.073699999999995 37.0 37.0 37.0 37.0 37.0 60-64 36.017 37.0 37.0 37.0 37.0 37.0 65-69 35.9332 37.0 37.0 37.0 37.0 37.0 70-74 35.9988 37.0 37.0 37.0 37.0 37.0 75-79 36.039300000000004 37.0 37.0 37.0 37.0 37.0 80-84 35.964999999999996 37.0 37.0 37.0 37.0 37.0 85-89 35.9034 37.0 37.0 37.0 37.0 37.0 90-94 35.83460000000001 37.0 37.0 37.0 37.0 37.0 95-99 35.8333 37.0 37.0 37.0 37.0 37.0 100-104 35.83800000000001 37.0 37.0 37.0 37.0 37.0 105-109 35.788 37.0 37.0 37.0 37.0 37.0 110-114 35.5692 37.0 37.0 37.0 37.0 37.0 115-119 35.5587 37.0 37.0 37.0 37.0 37.0 120-124 35.5347 37.0 37.0 37.0 37.0 37.0 125-129 35.3445 37.0 37.0 37.0 37.0 37.0 130-134 35.25430000000001 37.0 37.0 37.0 34.6 37.0 135-139 35.0634 37.0 37.0 37.0 27.4 37.0 140-144 34.8977 37.0 37.0 37.0 25.0 37.0 145-149 34.6866 37.0 37.0 37.0 25.0 37.0 150-151 34.5145 37.0 37.0 37.0 25.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.0 1101 2 0.0 1101 3 0.0 1101 4 0.0 1101 5 0.0 1101 6 0.0 1101 7 0.0 1101 8 0.0 1101 9 0.0 1101 10-14 0.0 1101 15-19 0.0 1101 20-24 0.0 1101 25-29 0.0 1101 30-34 0.0 1101 35-39 0.0 1101 40-44 0.0 1101 45-49 0.0 1101 50-54 0.0 1101 55-59 0.0 1101 60-64 0.0 1101 65-69 0.0 1101 70-74 0.0 1101 75-79 0.0 1101 80-84 0.0 1101 85-89 0.0 1101 90-94 0.0 1101 95-99 0.0 1101 100-104 0.0 1101 105-109 0.0 1101 110-114 0.0 1101 115-119 0.0 1101 120-124 0.0 1101 125-129 0.0 1101 130-134 0.0 1101 135-139 0.0 1101 140-144 0.0 1101 145-149 0.0 1101 150-151 0.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 18 2.0 19 0.0 20 1.0 21 1.0 22 10.0 23 7.0 24 12.0 25 14.0 26 14.0 27 12.0 28 24.0 29 30.0 30 25.0 31 31.0 32 74.0 33 91.0 34 160.0 35 429.0 36 2838.0 37 225.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 39.6797598198649 15.13635226419815 8.906680010007506 36.27720790592945 2 19.400000000000002 15.55 34.050000000000004 31.0 3 16.575 17.65 29.9 35.875 4 20.75 25.775 24.675 28.799999999999997 5 22.5 28.9 24.975 23.625 6 20.9 33.25 23.65 22.2 7 16.725 29.9 37.55 15.825 8 17.875 29.275000000000002 31.175000000000004 21.675 9 16.25 27.125 34.0 22.625 10-14 18.3968396839684 31.148114811481147 27.802780278027804 22.65226522652265 15-19 19.085 28.92 27.815 24.18 20-24 19.375 29.67 28.32 22.634999999999998 25-29 19.08 29.26 27.855 23.805 30-34 19.509999999999998 28.860000000000003 28.199999999999996 23.43 35-39 20.474999999999998 29.520000000000003 27.229999999999997 22.775000000000002 40-44 19.545 28.265 28.255000000000003 23.935000000000002 45-49 20.805 27.755000000000003 27.875 23.565 50-54 21.044999999999998 28.050000000000004 26.640000000000004 24.265 55-59 19.285 28.999999999999996 26.745 24.97 60-64 20.25 27.750000000000004 27.61 24.39 65-69 20.05 28.325 27.415 24.21 70-74 20.265 28.134999999999998 27.66 23.94 75-79 21.195 27.900000000000002 26.995 23.91 80-84 21.445 28.125 26.57 23.86 85-89 21.135 28.660000000000004 26.66 23.544999999999998 90-94 21.62 27.779999999999998 26.52 24.08 95-99 21.55 27.58 27.750000000000004 23.119999999999997 100-104 22.235 27.515 26.945000000000004 23.305 105-109 21.77 28.645 26.44 23.145 110-114 21.72 28.68 25.569999999999997 24.03 115-119 21.89 27.875 25.569999999999997 24.665 120-124 21.25 28.544999999999998 25.705 24.5 125-129 22.525000000000002 28.449999999999996 25.314999999999998 23.71 130-134 22.115000000000002 27.655 25.27 24.959999999999997 135-139 21.995 27.195000000000004 25.885 24.925 140-144 22.785 27.905 25.005 24.305 145-149 22.73 27.825 24.755 24.69 150-151 23.9875 28.549999999999997 23.799999999999997 23.6625 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 3.0 1 1.5 2 0.0 3 0.0 4 0.0 5 0.5 6 2.0 7 2.0 8 1.5 9 2.0 10 1.0 11 0.0 12 0.0 13 0.0 14 1.5 15 2.0 16 1.0 17 1.0 18 1.0 19 1.0 20 1.0 21 3.0 22 6.0 23 5.0 24 3.5 25 5.5 26 7.0 27 12.0 28 13.5 29 14.0 30 29.0 31 41.0 32 52.0 33 54.0 34 64.0 35 75.5 36 81.0 37 111.0 38 135.5 39 155.0 40 175.5 41 182.5 42 201.0 43 241.5 44 246.0 45 228.5 46 241.0 47 239.0 48 220.0 49 200.5 50 166.0 51 123.5 52 120.5 53 105.0 54 71.5 55 58.0 56 42.0 57 40.0 58 39.5 59 32.0 60 23.5 61 14.5 62 10.5 63 11.0 64 5.5 65 7.0 66 5.0 67 7.0 68 9.5 69 5.5 70 6.0 71 10.0 72 9.0 73 4.5 74 3.0 75 1.5 76 0.5 77 2.5 78 2.5 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.075 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.01 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150-151 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 60.675000000000004 #Duplication Level Percentage of deduplicated Percentage of total 1 64.02966625463536 38.85 2 21.054800164812526 25.55 3 8.281829419035846 15.075 4 3.4610630407911 8.4 5 1.3185002060156572 4.0 6 0.865265760197775 3.15 7 0.3708281829419036 1.575 8 0.3296250515039143 1.6 9 0.08240626287597858 0.44999999999999996 >10 0.20601565718994644 1.35 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GGTTTGGCTTATCGAGTTTCCTTAACGAAATACTGGTTGGTCCACAAGGG 12 0.3 No Hit GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCAAGCAATCTCGGTT 11 0.27499999999999997 TruSeq Adapter, Index 1 (97% over 37bp) GTCCACAGCATCTTGTTCTTTCTTCTTCGGATCTTGTTGCTTCTTTGGCT 11 0.27499999999999997 No Hit GTTTCCTTGATCATGGAAAAAGTTTGCTGGGCGGCTCAGTGGTTCATCCA 10 0.25 No Hit GTCGAGGGAAGAACTGACGGTTTATTGGACGGAAGGTCACAACTGTTAAC 10 0.25 No Hit GCACCGTGTCTGAACTTCGTATTTACTCAATTCTAAGAACAGCAATCTCT 9 0.22499999999999998 No Hit CTCATTATATCTCTGCATTTTTCCATGTTTTCACTTGAAGAAAATGGATT 9 0.22499999999999998 No Hit CCACAAGAACTCCAGCAGAACTTGTAACGTTGATGAAGATTTTATTTAGG 8 0.2 No Hit CCTTCAACTTACTTGCCTCCCAAAATACCAAGAAAAAGGTGGGGGGGAAA 8 0.2 No Hit TGCTGCTACATCTGTCTATGTTTGATCAAGAAGATCATTATTTGGGTAAT 8 0.2 No Hit AGAGCACATACCAAACCTTAGATTCTCTGCACAAGTTTGGATCAGAAAAC 8 0.2 No Hit CTTATCCTACACCTCTCAAGTCATTTCACAAAGTCGGACTAGAGTCAAGC 8 0.2 No Hit GGCAGTTTCCAGAAACGTGTATCACATCTAGGCATGGAATCTTATGCCAG 8 0.2 No Hit TATCTTTGCCACGACCGCCCATGATGGCATCCTTTGCAGACTTGAAGGTG 8 0.2 No Hit GTGACTTCGGTCCATTCCCCGTCACCTTGATCACTATCTTTTGCTTCATG 8 0.2 No Hit CTCATCACTCACAAGCAAGTCGTGGCGTAGGAATTGAATTAATGAACTTG 7 0.17500000000000002 No Hit GCAGATTGAACAGAAGAGTAGACACAGTAGATGATATATAGTTCTTGGGG 7 0.17500000000000002 No Hit TGGGCCTTGTCTTCATTGAGAGGCTTTAACCTCCCTAGCTCCTTCATCTG 7 0.17500000000000002 No Hit GACAGCATAAATAATTCCAGGGATGTAGCCTAAGATGGTGAGGAGCAAGC 7 0.17500000000000002 No Hit GGTGGAACAAGAGGAGCCTTCGTAAGGTAACTGAGAATAGTAGCAACAGG 7 0.17500000000000002 No Hit TACACGAAGAACATCAACCCCTGGCCCATGACAAAGTATAGCCATAATGG 7 0.17500000000000002 No Hit CAACAAACAAGACCAGACACTAAGCAAAAGTTCAGAATCCTTGGAGCTTA 7 0.17500000000000002 No Hit AGGTTCTGCAATAGAGATTAAAATAGGGCAGGGAGCTAAGCCTGGAATTG 7 0.17500000000000002 No Hit GTTCGCCCAGTGGGCTCCGCGCGTGAGCACTTCCCACTGATCCAAGCGTC 7 0.17500000000000002 No Hit GTCCTGCTGTCTTAATCGACCAACACCCTTTGTGGGTTCTAGGTTAGCGC 6 0.15 No Hit GTAAGACCATCAGCAAGAGAAGCGACAAGAAGCTTAGCGTTTGGGAGAGA 6 0.15 No Hit GCATCGTATTTCAGAACATGAAACTTCATCCCATCAGATCTTCAGATTTT 6 0.15 No Hit CATAATAATCTCTCAATGGCAAAGTCTTCAAGAAAGCACCAAGTCCGCAC 6 0.15 No Hit GTAGCAATACAATTACTGATGTTATTTATAGGAACTTCAAAATTTTTCAA 6 0.15 No Hit CAGCAATGCCCATCCGTTCCTGTGGCGACTCGACAGAACAGGAGATTCCA 6 0.15 No Hit CCTGCAACCTCTACTGTGGCCTAACCCTATGGCTCAACACCATTTCCCTA 6 0.15 No Hit AGAATACGATGTCTTCTGATGCCTCTCAGTATGCCTCCTGTTGTAATAAG 6 0.15 No Hit AGCAGCAACTTCTTTCCCAGTCATTATCTCCAGTGTGAGAATTCCAAATG 6 0.15 No Hit CCCTTTACTCTGCTTACCATCCAAGGCAATCTTATATAGATAGAATGCAG 6 0.15 No Hit CTCATACACAACAGCCTGGTACGTGGCATTAGCCCTTCCTTTCACTGCCA 6 0.15 No Hit CTTCTTGAATACCAATGGGAATTTGATTTTGGAGTTGTGGAACTGCTTGG 6 0.15 No Hit CTACCGTGCAATCCTTGAACTGACTGAGCTTAATTAGTTCCCCCCCTGGC 6 0.15 No Hit CTTGCATGTGAAAAGAAGCATGAACACTATCTACCCTATCTTCCATTCCC 6 0.15 No Hit CCCCAGGTTAGCTCTTTCAGCTTTTCATATGGCTCAGGAACACTATATCG 6 0.15 No Hit GTTCGGATCTATACATTTCAGCTCCATCGCCATTCATGACCGGAAGCTTT 6 0.15 No Hit CCTCACAAGATTCTACTGGATCAGTTCATTTTCCTCCAGCTTTCAGCATC 6 0.15 No Hit GCCATTTTACAATTTGAACAGTTCAGGACGCGAAAGAAATTAATTTCTAC 6 0.15 No Hit CTTGATTGTCTTATCCCTTGAAGCAGAAACGATCTGTCGGTTATCAAAAG 6 0.15 No Hit ATAATTTAAAGCTGCCAGCCAGGTGCATTGCTTCCGGTTCCCGTCCCTGT 6 0.15 No Hit CCTAGAGTCTCAATGAACCTTGGAAAGAATGGTTTGGTGTTGGTGTTGGA 6 0.15 No Hit GCATTTAATTTTTACAATGAAAGAACAATTAAACTTGACAACTTCACTCG 5 0.125 No Hit ACTCCAAAGTCGATCTTACCGATAATCTAGAGGACCTAAACCAGATTATT 5 0.125 No Hit CTCCTAATGAAATAGGTATCGATCCCTTCAATACAATCATCACATAATTG 5 0.125 No Hit ACGCCCTCCTGTTTCTTCCCTCATGTCAACTGTGGATGTCGTGATCTTTT 5 0.125 No Hit CTTCCACATGGGCTTGGTAATTTACCTAAAACTCAGATCAAGAGCAAGCA 5 0.125 No Hit GGCGTATGTTACTAGCTGCACCTTGTCCTCCATGCCTGCTGCAAGCTGGT 5 0.125 No Hit GGCGCCAAAAACACTGCTGCACTCAAGATTCTGTTCTGTATTTCTGGCTG 5 0.125 No Hit TTTAGAAACCCTTTGAATCTCCCCAATCTTCTCATTGAAAGCGTGCTGTG 5 0.125 No Hit CAGTGGTTTTGCTAAGTTCATGTCCTCCCTTGCCAAGATCATCAGGATCT 5 0.125 No Hit CCCTTCGATCCTCACCACAGCTGGCATGTCCCCAAATTTCATTCGGACAC 5 0.125 No Hit TTTAACAGCAACGGGAACTTAATCCCTTATTTTACATGGTATTGAATTTG 5 0.125 No Hit CTCTATTTGAACGTTCATAAAAATCCATCAGGTTTGCATTCCACAAGTTT 5 0.125 No Hit GTCTCAGGCTCCTCCCAAGCATTCCAAAGATGAAAACAGAAGCAGTCAGG 5 0.125 No Hit CTCAAATTCAAGCTTATCATGCACATTTCTGGTTCTGGCATTTCATTATC 5 0.125 No Hit TCTCAAACAACCAAAAAACATAACAAGCTCGGCTTGTAAAATTCCAAAAC 5 0.125 No Hit GGGGAAGAAAAGAAAGAAAAATGATAGGGAGATTTCTTCTTTTTTTGGTT 5 0.125 No Hit GCCTCTCCTTTTTGTTGATATCATGAAGCACACAAACATCCGAAGCAGTT 5 0.125 No Hit GCCACAACAGGATTCAAATAACTACCTATCCTCTAAGTAATTATATCATT 5 0.125 No Hit ACTGAGCTGATCATAGCCTTTCTTGATCCCAACCAAGATATTAAAATGAA 5 0.125 No Hit AGGTATTAGAATAACAATCAAGTCCCCACAAGAGATCTATATTCTATAGT 5 0.125 No Hit ATTCAGTACAAGAGACCCTGCATAGTTACTTTCTAATTTACTTGGGAAGC 5 0.125 No Hit ATCACAAACACATTACATCACAAACACATTATGTTGCATTATAAAATTTG 5 0.125 No Hit CTTCACAATGATAGGAAGAGCCGACATCGAAGGATCAAAAAGCAACGTCG 5 0.125 No Hit GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCAAGCAATCGCGGTT 5 0.125 TruSeq Adapter, Index 1 (97% over 37bp) ATCTGGTCTTGGAAATTCTCTTCTCGGCAAGCTTCAAAATGTTCTCCGCC 5 0.125 No Hit CGGCCAAGCCAAACTCCCCACCTGACAATGTCTTCCGCCCGGATCGGCCG 5 0.125 No Hit ACAGGAATAGGATTTTGCTGGTCGGATTGACCATCCATGGTTGGATAGCC 5 0.125 No Hit TTCTTCTTCTTGCATGCTGATGAAGATGTTGGCATTGGCGTTGAAGTCTC 5 0.125 No Hit CCAGTCCCGAACCCGTCGGCTGTCGGTGGACTGCTCGAGCTGCTCCCGCG 5 0.125 No Hit CTGACAAAATTGAAGAGGATCTGATGTTCTTGGCAGAAACAGAAATTTCA 5 0.125 No Hit GTTTTTGCATTCCCCATGCATGTACCACAGAAAAATTTCCTTGTCATGAG 5 0.125 No Hit GAGAGACTGCTTCAGGAAGTGCTCTGGATATAAACCTAGTACTTGTTGGT 5 0.125 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0125 0.0 0.0 0.0 0.0 64-65 0.05 0.0 0.0 0.0 0.0 66-67 0.05 0.0 0.0 0.0 0.0 68-69 0.05 0.0 0.0 0.0 0.0 70-71 0.05 0.0 0.0 0.0 0.0 72-73 0.05 0.0 0.0 0.0 0.0 74-75 0.05 0.0 0.0 0.0 0.0 76-77 0.1375 0.0 0.0 0.0 0.0 78-79 0.1875 0.0 0.0 0.0 0.0 80-81 0.2875 0.0 0.0 0.0 0.0 82-83 0.3625 0.0 0.0 0.0 0.0 84-85 0.55 0.0 0.0 0.0 0.0 86-87 0.7375 0.0 0.0 0.0 0.0 88-89 0.9750000000000001 0.0 0.0 0.0 0.0 90-91 1.125 0.0 0.0 0.0 0.0 92-93 1.2625 0.0 0.0 0.0 0.0 94-95 1.3875000000000002 0.0 0.0 0.0 0.0 96-97 1.5499999999999998 0.0 0.0 0.0 0.0 98-99 1.95 0.0 0.0 0.0 0.0 100-101 2.775 0.0 0.0 0.0 0.0 102-103 3.325 0.0 0.0 0.0 0.0 104-105 3.9875 0.0 0.0 0.0 0.0 106-107 4.7375 0.0 0.0 0.0 0.0 108-109 5.425 0.0 0.0 0.0 0.0 110-111 6.050000000000001 0.0 0.0 0.0 0.0 112-113 6.6 0.0 0.0 0.0 0.0 114-115 7.4375 0.0 0.0 0.0 0.0 116-117 8.0875 0.0 0.0 0.0 0.0 118-119 8.75 0.0 0.0 0.0 0.0 120-121 9.65 0.0 0.0 0.0 0.0 122-123 10.5625 0.0 0.0 0.0 0.0 124-125 11.3375 0.0 0.0 0.0 0.0 126-127 12.3375 0.0 0.0 0.0 0.0 128-129 13.125 0.0 0.0 0.0 0.0 130-131 13.7125 0.0 0.0 0.0 0.0 132-133 14.787500000000001 0.0 0.0 0.0 0.0 134-135 15.9625 0.0 0.0 0.0 0.0 136-137 17.1 0.0 0.0 0.0 0.0 138-139 18.012500000000003 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTTTCAA 10 0.006830828 145.0 5 CAATCCC 10 0.006830828 145.0 9 GCTTTCA 10 0.006830828 145.0 4 >>END_MODULE SRR26075396 read2 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR26075396_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.44175 37.0 37.0 37.0 37.0 37.0 2 36.398 37.0 37.0 37.0 37.0 37.0 3 36.3835 37.0 37.0 37.0 37.0 37.0 4 36.47 37.0 37.0 37.0 37.0 37.0 5 36.5775 37.0 37.0 37.0 37.0 37.0 6 36.445 37.0 37.0 37.0 37.0 37.0 7 36.3955 37.0 37.0 37.0 37.0 37.0 8 36.4985 37.0 37.0 37.0 37.0 37.0 9 36.4575 37.0 37.0 37.0 37.0 37.0 10-14 36.4253 37.0 37.0 37.0 37.0 37.0 15-19 36.430699999999995 37.0 37.0 37.0 37.0 37.0 20-24 36.29729999999999 37.0 37.0 37.0 37.0 37.0 25-29 36.2199 37.0 37.0 37.0 37.0 37.0 30-34 36.104499999999994 37.0 37.0 37.0 37.0 37.0 35-39 36.0673 37.0 37.0 37.0 37.0 37.0 40-44 36.008799999999994 37.0 37.0 37.0 37.0 37.0 45-49 35.8763 37.0 37.0 37.0 37.0 37.0 50-54 35.812599999999996 37.0 37.0 37.0 37.0 37.0 55-59 35.8049 37.0 37.0 37.0 37.0 37.0 60-64 35.9075 37.0 37.0 37.0 37.0 37.0 65-69 35.7641 37.0 37.0 37.0 37.0 37.0 70-74 35.7614 37.0 37.0 37.0 37.0 37.0 75-79 35.6394 37.0 37.0 37.0 37.0 37.0 80-84 35.7803 37.0 37.0 37.0 37.0 37.0 85-89 35.683299999999996 37.0 37.0 37.0 37.0 37.0 90-94 35.587 37.0 37.0 37.0 37.0 37.0 95-99 35.693200000000004 37.0 37.0 37.0 37.0 37.0 100-104 35.6434 37.0 37.0 37.0 37.0 37.0 105-109 35.5184 37.0 37.0 37.0 37.0 37.0 110-114 35.538 37.0 37.0 37.0 37.0 37.0 115-119 35.4519 37.0 37.0 37.0 37.0 37.0 120-124 35.41045 37.0 37.0 37.0 37.0 37.0 125-129 35.343999999999994 37.0 37.0 37.0 37.0 37.0 130-134 35.295100000000005 37.0 37.0 37.0 34.6 37.0 135-139 35.062149999999995 37.0 37.0 37.0 27.4 37.0 140-144 35.14315 37.0 37.0 37.0 27.4 37.0 145-149 35.06875 37.0 37.0 37.0 25.0 37.0 150-151 34.67875 37.0 37.0 37.0 25.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.0 1101 2 0.0 1101 3 0.0 1101 4 0.0 1101 5 0.0 1101 6 0.0 1101 7 0.0 1101 8 0.0 1101 9 0.0 1101 10-14 0.0 1101 15-19 0.0 1101 20-24 0.0 1101 25-29 0.0 1101 30-34 0.0 1101 35-39 0.0 1101 40-44 0.0 1101 45-49 0.0 1101 50-54 0.0 1101 55-59 0.0 1101 60-64 0.0 1101 65-69 0.0 1101 70-74 0.0 1101 75-79 0.0 1101 80-84 0.0 1101 85-89 0.0 1101 90-94 0.0 1101 95-99 0.0 1101 100-104 0.0 1101 105-109 0.0 1101 110-114 0.0 1101 115-119 0.0 1101 120-124 0.0 1101 125-129 0.0 1101 130-134 0.0 1101 135-139 0.0 1101 140-144 0.0 1101 145-149 0.0 1101 150-151 0.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 13 3.0 14 2.0 15 2.0 16 4.0 17 7.0 18 6.0 19 2.0 20 10.0 21 8.0 22 8.0 23 7.0 24 11.0 25 10.0 26 15.0 27 14.0 28 12.0 29 12.0 30 21.0 31 19.0 32 36.0 33 75.0 34 193.0 35 663.0 36 2625.0 37 235.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.63615903975994 22.455613903475868 11.677919479869967 21.230307576894223 2 29.925 26.85 26.650000000000002 16.575 3 23.35 28.025 29.725 18.9 4 25.85 31.674999999999997 23.0 19.475 5 26.674999999999997 34.575 20.5 18.25 6 23.075000000000003 38.925 20.125 17.875 7 23.200000000000003 23.025000000000002 36.425000000000004 17.349999999999998 8 24.349999999999998 27.700000000000003 23.549999999999997 24.4 9 26.55 25.775 27.275 20.4 10-14 26.55 28.035 25.22 20.195 15-19 25.369999999999997 28.025 26.445 20.16 20-24 25.580000000000002 27.57 26.075 20.775 25-29 25.645 27.985 25.83 20.54 30-34 25.040000000000003 28.09 26.465 20.405 35-39 25.535000000000004 28.410000000000004 25.8 20.255000000000003 40-44 25.81 27.415 26.540000000000003 20.235 45-49 24.955 28.01 26.96 20.075000000000003 50-54 25.019999999999996 27.334999999999997 27.16 20.485 55-59 25.155 26.87 28.03 19.945 60-64 26.200000000000003 27.485 26.02 20.294999999999998 65-69 25.729999999999997 28.000000000000004 25.629999999999995 20.64 70-74 24.8 27.93 26.729999999999997 20.54 75-79 24.375 28.189999999999998 27.01 20.424999999999997 80-84 24.834999999999997 28.09 26.685 20.39 85-89 24.915000000000003 28.38 26.32 20.385 90-94 25.135 27.810000000000002 27.0 20.055 95-99 26.02 27.185 26.905 19.89 100-104 25.35 28.29 26.369999999999997 19.99 105-109 25.869999999999997 28.084999999999997 26.16 19.885 110-114 26.58 28.03 25.869999999999997 19.52 115-119 26.035000000000004 27.950000000000003 26.669999999999998 19.345000000000002 120-124 26.50132506625331 28.221411070553525 26.181309065453274 19.095954797739886 125-129 26.14 29.154999999999998 25.795 18.91 130-134 27.195000000000004 28.27 26.21 18.325 135-139 27.524128619292892 27.639145871880782 26.739010851627743 18.09771465719858 140-144 28.019202880432065 28.144221633244985 25.468820323048458 18.36775516327449 145-149 28.43710927731933 29.067266816704173 25.03125781445361 17.46436609152288 150-151 27.84446111527882 30.195048762190545 23.293323330832706 18.667166791697927 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.5 3 0.5 4 0.5 5 0.5 6 1.0 7 1.0 8 0.0 9 1.0 10 1.5 11 1.5 12 1.5 13 1.5 14 1.5 15 1.0 16 2.0 17 1.5 18 1.0 19 1.0 20 0.0 21 0.0 22 0.0 23 0.5 24 1.5 25 1.5 26 0.5 27 2.0 28 4.0 29 7.5 30 11.0 31 14.5 32 20.5 33 26.5 34 34.5 35 38.0 36 65.0 37 92.5 38 91.0 39 119.0 40 176.5 41 223.5 42 254.5 43 249.5 44 249.5 45 258.5 46 279.5 47 277.5 48 233.5 49 206.5 50 168.0 51 137.5 52 123.5 53 108.0 54 89.0 55 79.0 56 56.5 57 37.0 58 36.0 59 33.5 60 31.0 61 23.0 62 18.0 63 11.0 64 5.5 65 3.5 66 4.0 67 5.5 68 3.5 69 3.0 70 3.0 71 5.0 72 5.0 73 1.5 74 1.0 75 0.5 76 0.0 77 3.5 78 4.0 79 1.0 80 0.5 81 0.0 82 1.0 83 2.5 84 2.0 85 1.0 86 2.0 87 3.0 88 2.5 89 2.0 90 4.0 91 3.5 92 1.0 93 1.5 94 2.5 95 1.5 96 0.0 97 1.0 98 1.5 99 1.0 100 4.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.025 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.005 125-129 0.0 130-134 0.0 135-139 0.015 140-144 0.015 145-149 0.025 150-151 0.025 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 60.650000000000006 #Duplication Level Percentage of deduplicated Percentage of total 1 64.4270403957131 39.074999999999996 2 20.8161582852432 25.25 3 8.285243198680957 15.075 4 3.215169002473207 7.8 5 1.4014839241549877 4.25 6 0.9480626545754328 3.45 7 0.2885408079142622 1.225 8 0.2885408079142622 1.4000000000000001 9 0.08244023083264633 0.44999999999999996 >10 0.24732069249793898 2.025 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG 19 0.475 No Hit GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGT 17 0.42500000000000004 No Hit GTGATTGGGGACGTTGATCCAGTAGACATAGTGAGCAAACTGAGAAAGCT 13 0.325 No Hit TACCGATTCAAATGCTTGGAAGCAAGTCAGTTGATCTGTTGAGAAAGAAG 12 0.3 No Hit TCAAAATGGCCGATAAACTCGCCGGTACCATCACTGTCACTGTTCTTTTC 10 0.25 No Hit GCACAAACCAGTCATGATCCAAATTGGAATTTTGAGCTGATGGACCAATC 10 0.25 No Hit GAAACTTCAGGTCATTACAGAGCTTTCCTTCTCGCTCTCTTGGGGCCCAA 9 0.22499999999999998 No Hit AGATTACTGCCCAGACCCCAGCAAATATGGCCATGAAATAGGCCCGCTGG 9 0.22499999999999998 No Hit TATGAAGATGTGAATCACTACGAGTCAAAGGCACCACATCCAAAAATGGC 8 0.2 No Hit GGAACACTCAATGTTCTTGGCTCATGTGCAAAACACCCGTCAATCAGACG 8 0.2 No Hit TAAACGAAGAAGGTGAAGCTGAAACCAGGGTGGAGACTGTCGATCACCGG 8 0.2 No Hit TTTCGGCACTTTCTCTAGCTTTCCCACTCTAGAGTCATGGAAGGTTGTGT 8 0.2 No Hit AAACCATCACTTTTGGTGTCTCCTGGACTGTAAACAGCTCCTCATTCCCT 8 0.2 No Hit GACGAAGAAGAGATATTATTGCACTCTGTGTGCTAGAAAATTAGCTGTCC 8 0.2 No Hit GCTCTGAATGTCAAAGTGAAGAAATTCAACCAAGCGCGGGTAAACGGCGG 8 0.2 No Hit GCACGCCACAGGCGTCATACTTCCCAAGAAGCGGCCATAGCCCAGATGCG 7 0.17500000000000002 No Hit GAACAAGGCTAGCAGGGAGAGGAAATTTGCTAGGAGGGAAGAACGATTAG 7 0.17500000000000002 No Hit GTTCGAAATAAAGCATACGGAGACTTCCTTAACGATAATTTTAATGCAAC 7 0.17500000000000002 No Hit CGAGGAAGGGGACACTTGCACCACAAGGCACGGCCTTCCAGGAGGGCAAC 7 0.17500000000000002 No Hit AAGCTGTCAACGGCAAGCCTGCCTGAGACTCCAAAAAGTAAACCAAACTT 7 0.17500000000000002 No Hit TGTCATGGTGAGGAGCTGGTGAGATAGCATCACTTCCCTCAGGAATCATT 7 0.17500000000000002 No Hit ATGAGTACACCTCAGAGATATTCATGGGTGGCCAAAACACCATTGTGATG 7 0.17500000000000002 No Hit GGCACATCGTTGGGACTAAAGGTTACATGGCTCCCGAGTACTTGGAAAAT 6 0.15 No Hit AGACAATCACAAAGCGAGGCGCCGGGAGGGAGAATCTGGAGTACACTGAT 6 0.15 No Hit CCGAAAAAAAGGGATTGAACTCTTGGAGAAAAAAGTAGAGACATTTTATG 6 0.15 No Hit GCCATATGGTGGGCAAGTGGAGGAGGGTGTGGTGAGGACACATCCAGGTG 6 0.15 No Hit GCTTAGGACATTCTCTTCTTGCCTACAAAAGCGCGTTGCAAGGAATAGCA 6 0.15 No Hit GGAAGATGGGGCGGTGGCCAAAGAAGATTCTGAGGCCTACTCTGCATGGG 6 0.15 No Hit GCAATACATTATTTTTCAGGGGAGTACTTTAGTAGAGAATTAATAAGAAG 6 0.15 No Hit GAGAAAGTGTTGCAGATTGCAGATCCTACTCTTCTTCAAATAAAATTTGA 6 0.15 No Hit AGATGAGAGTGTGACCACTTGGAATCACAGAGATAGATTGATAAGGATGG 6 0.15 No Hit AAATTTGTTGATGCAGTGTTGAATGAGTGGCATAAAACAGTGATGGATTT 6 0.15 No Hit GGCTGATCGTGGTTTTACTAGGCTAGACTAGCGTACGAGCACTATGGTCA 6 0.15 No Hit CCTTTGTATAGAGAGCGAGCCGCTAGCTAGCTGCTCTGCAGGACGCTGTT 6 0.15 No Hit GTGACCTCTTTTGTGCATCTCATCGCTACTCAGACAAACATGACTGCCCA 6 0.15 No Hit AGAGCCGAACTGGTTATCACAACATGTACAAGGAATACCGAGACACGACC 6 0.15 No Hit GAATGCCACTGCATTTTAGCACAAAGAACGGTGATCTTTACATCACGTTT 6 0.15 No Hit GCTGCATTTGTGTGCAATCCAACACTCTGGCTGGTTTCGCCTGTAGAACC 6 0.15 No Hit GGTACCTAATGTTGGTGGATCAGTGTACACTACTCACGTACCAATTATTG 6 0.15 No Hit CATGGGTGGACAACAAATATGATTATCACTGAAAGGAAAAATTACATGGG 6 0.15 No Hit AGGAAGAAGGGATGTCATCGGAGTCTACAACCAATGTTTGCAGCTGCAGC 6 0.15 No Hit GCTACTGGTGTCTCTGCTCGCCGATTCGTCGGCCACACCAAGGATGTGTT 6 0.15 No Hit CTGAGCCACACCTAACTGTACTGTAAAATTGCAGGTTGTTTCTGTACAGA 6 0.15 No Hit AGATCGCGGAGTTCGCCGTTGCAGAGCATAACAAGGAAGCCAAATCAAAT 6 0.15 No Hit GACGGCGCGGCTGCTTCGTTGAGCCGCGCCACGGAATCGAGAGCTCCAAG 6 0.15 No Hit GAAATACCACTACTTTTAACGTTATTTTACTTATTCCGTGAATCGGAGGC 5 0.125 No Hit TGAAGGTATTGCTGGAGGTCTTGATGAGGAAGATGATGGTGCTGGTGATG 5 0.125 No Hit GGACTCACTGTTACCTACAAGATTGTGTTCCACCTCTACTGGCCCTCCTA 5 0.125 No Hit GGAGGAAGTGCTCCATCCGAGGATGCAACTGCCAATGAATGAGAAGTTAC 5 0.125 No Hit CATGATTTTGCTATCACAGAGAGGTTTGTTGTGGTACCTGATCAGCAAGT 5 0.125 No Hit CGACATTCAGAGCACTGGGCAGAAATCACATTGCGTGAGCATCCGCAGGG 5 0.125 No Hit GGCCAATGGAGTGGTCTACTGTTCCATATACAGGGTCACAAGGACCTGGA 5 0.125 No Hit CAACCACCACAACCGAACTTCTCTCGTCCTTATCTCCATTCTTCCATAGC 5 0.125 No Hit AGGATATCTTGTGTTTTGTTCTCCTAGAGAATTTTCTTCTTTCTTCTTAG 5 0.125 No Hit CAGCAAAGGGCTGTACTTGACAAGCGGATCATGAAGATTTCAGAACTAGG 5 0.125 No Hit CTTGACTATTTTCTACAATGGAACCGTTGCCGTTTTCAATGTCCCTCGAG 5 0.125 No Hit GTATTACAGGCCTGCTAATTATGTAGCAGAGATCGATCCAGCTGAACTTA 5 0.125 No Hit GATGTTCTGACACTTGTTGTTGTAGCAGGCTGTTGCTATGAAATTTATGT 5 0.125 No Hit CATCACAGCAGCAGCAGATTCAGGCAATAACAAGAAGAACCGAATCCAAG 5 0.125 No Hit GCCCGTCTTCATCATCACTTGCCGCACCCAGGAGGTGCATGTCTACCGCA 5 0.125 No Hit CCACAAATGGCTGCATGTCAACTGGCCCGCATTTTAATCCTGTAGGCAAA 5 0.125 No Hit GTTCTATCGGGTAAAGCCAATGATTAGAGGCATCGGGGGCGCAACGCCCT 5 0.125 No Hit ATTACAACAAGTTGCCCAAAGAGAAAAACAGAAGACTCCTTCAAAATCTT 5 0.125 No Hit GAAATTACTACTTTTAAATCCTTCTCTTTGTGAACAAATCAGACCTCTAA 5 0.125 No Hit GGCCGAGTCCCACATTGAAGAACAGAGGCATCGAAGCATTTCAATCTCAT 5 0.125 No Hit ATTTGGCTTTCCAGCATTTTTCACGGATAGGGAATATGCAGAGAGCATTT 5 0.125 No Hit GGCGAAGATTAAATACAACTCTTGGAGTGGTTCTTGATGACATCAATTAA 5 0.125 No Hit GTGATTCAGCACAGGTGACAACAGCCTCATGAAGTGCTCTCTCTCCCTCT 5 0.125 No Hit GAAGGCCAAGATCCAGGATAAGGAAGGTATTCCCCCAGACCAACAAAGGT 5 0.125 No Hit GTTACGCGGCTGCTGCACCGATCAGTGCTGCTGTAACAGCGAGCTTTGGT 5 0.125 No Hit AGTGGTTCGGCTGAGAAGAAGCAGGTCTCCAACAATTATCACAGAGCTCA 5 0.125 No Hit CACCAATCGAGAAAATGGATAGGGTGTGGAGGGAGCACGGGTTTTGGAAC 5 0.125 No Hit GGGGCTTTGAAGAATGTTTGCATTGTTGGGAATTCTGGGCACCCTTTAAT 5 0.125 No Hit AGCCTTCGTAGGAGAAGCAAGAATTAATCCTGTTTCTTGGGGGGGGGGGG 5 0.125 No Hit CTTCACCTTGGCCGATCTGCACCACCTCCCTAACATCCAGGCCTTGTTGG 5 0.125 No Hit GTTTGCTGTGGAACCCTAGATATGTTTTGAGCAGTGTAACTAGTGATCAA 5 0.125 No Hit GTCAGACCGTGGCGGTTGGTGTCATTAAGAGTGTTGAGAAGAAGGATCCA 5 0.125 No Hit AAACAAGAAGGATAAGGAGAAGAAAGAGAAAAAGCACAGGGATGGAGCAG 5 0.125 No Hit ATTTCCACAAACGTCAGCCTTGATGGAGTTGACACCTCCTCCATCCTCTC 5 0.125 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0125 0.0 0.0 0.0 0.0 64-65 0.05 0.0 0.0 0.0 0.0 66-67 0.05 0.0 0.0 0.0 0.0 68-69 0.05 0.0 0.0 0.0 0.0 70-71 0.05 0.0 0.0 0.0 0.0 72-73 0.05 0.0 0.0 0.0 0.0 74-75 0.05 0.0 0.0 0.0 0.0 76-77 0.1375 0.0 0.0 0.0 0.0 78-79 0.1875 0.0 0.0 0.0 0.0 80-81 0.2875 0.0 0.0 0.0 0.0 82-83 0.3625 0.0 0.0 0.0 0.0 84-85 0.5249999999999999 0.0 0.0 0.0 0.0 86-87 0.7 0.0 0.0 0.0 0.0 88-89 0.925 0.0 0.0 0.0 0.0 90-91 1.0750000000000002 0.0 0.0 0.0 0.0 92-93 1.2125 0.0 0.0 0.0 0.0 94-95 1.35 0.0 0.0 0.0 0.0 96-97 1.525 0.0 0.0 0.0 0.0 98-99 1.9375 0.0 0.0 0.0 0.0 100-101 2.8 0.0 0.0 0.0 0.0 102-103 3.4625 0.0 0.0 0.0 0.0 104-105 4.2125 0.0 0.0 0.0 0.0 106-107 4.9625 0.0 0.0 0.0 0.0 108-109 5.725 0.0 0.0 0.0 0.0 110-111 6.375 0.0 0.0 0.0 0.0 112-113 6.925 0.0 0.0 0.0 0.0 114-115 7.7625 0.0 0.0 0.0 0.0 116-117 8.4125 0.0 0.0 0.0 0.0 118-119 9.1 0.0 0.0 0.0 0.0 120-121 10.0125 0.0 0.0 0.0 0.0 122-123 10.9625 0.0 0.0 0.0 0.0 124-125 11.75 0.0 0.0 0.0 0.0 126-127 12.7625 0.0 0.0 0.0 0.0 128-129 13.5875 0.0 0.0 0.0 0.0 130-131 14.225000000000001 0.0 0.0 0.0 0.0 132-133 15.25 0.0 0.0 0.0 0.0 134-135 16.375 0.0 0.0 0.0 0.0 136-137 17.4875 0.0 0.0 0.0 0.0 138-139 18.450000000000003 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TGCTGTC 10 0.006830828 145.0 6 GCTGTCT 10 0.006830828 145.0 7 AAAGATC 40 0.005621335 54.375 145 AAAAAAA 165 5.380233E-4 8.787879 95-99 >>END_MODULE Read 677440 spots for SRR26075396.sra Written 677440 spots for SRR26075396.sra Read 677440 spots for SRR26075396.sra Written 677440 spots for SRR26075396.sra Read 677440 spots for SRR26075396.sra Written 677440 spots for SRR26075396.sra Read 677440 spots for SRR26075396.sra Written 677440 spots for SRR26075396.sra Read 677440 spots for SRR26075396.sra Written 677440 spots for SRR26075396.sra Read 677440 spots for SRR26075396.sra Written 677440 spots for SRR26075396.sra Read 677440 spots for SRR26075396.sra Written 677440 spots for SRR26075396.sra Read 677440 spots for SRR26075396.sra Written 677440 spots for SRR26075396.sra Read 677440 spots for SRR26075396.sra Written 677440 spots for SRR26075396.sra Read 677440 spots for SRR26075396.sra Written 677440 spots for SRR26075396.sra Read 677440 spots for SRR26075396.sra Written 677440 spots for SRR26075396.sra Read 677440 spots for SRR26075396.sra Written 677440 spots for SRR26075396.sra Read 677440 spots for SRR26075396.sra Written 677440 spots for SRR26075396.sra Read 677440 spots for SRR26075396.sra Written 677440 spots for SRR26075396.sra Read 677440 spots for SRR26075396.sra Written 677440 spots for SRR26075396.sra Read 677452 spots for SRR26075396.sra Written 677452 spots for SRR26075396.sra Read 677440 spots for SRR26075396.sra Written 677440 spots for SRR26075396.sra Read 677440 spots for SRR26075396.sra Written 677440 spots for SRR26075396.sra Read 677440 spots for SRR26075396.sra Written 677440 spots for SRR26075396.sra Read 677440 spots for SRR26075396.sra Written 677440 spots for SRR26075396.sra SRR ids: ['SRR26075396.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_80wstqry SRR26075396.sra spots: 13548812 blocks: [[1, 677440], [677441, 1354880], [1354881, 2032320], [2032321, 2709760], [2709761, 3387200], [3387201, 4064640], [4064641, 4742080], [4742081, 5419520], [5419521, 6096960], [6096961, 6774400], [6774401, 7451840], [7451841, 8129280], [8129281, 8806720], [8806721, 9484160], [9484161, 10161600], [10161601, 10839040], [10839041, 11516480], [11516481, 12193920], [12193921, 12871360], [12871361, 13548812]] SRR26075396 file size 4996721 SRR26075396 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o SRR26075396 SRR26075396_1.fastq SRR26075396_2.fastq Input file: SRR26075396_1.fastq Paired file: SRR26075396_2.fastq trimmed: SRR26075396-trimmed-pair1.fastq, SRR26075396-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Wed Feb 12 00:33:31 2025 >> started Wed Feb 12 00:33:56 2025 >> done (25.358s) 13548812 read pairs processed; of these: 62 ( 0.00%) short read pairs filtered out after trimming by size control 85000 ( 0.63%) empty read pairs filtered out after trimming by size control 13463750 (99.37%) read pairs available; of these: 3662576 (27.20%) trimmed read pairs available after processing 9801174 (72.80%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 18 6 0.00% 19 9 0.00% 20 8 0.00% 21 13 0.00% 22 16 0.00% 23 13 0.00% 24 11 0.00% 25 18 0.00% 26 21 0.00% 27 27 0.00% 28 17 0.00% 29 18 0.00% 30 29 0.00% 31 28 0.00% 32 17 0.00% 33 21 0.00% 34 16 0.00% 35 41 0.00% 36 30 0.00% 37 38 0.00% 38 26 0.00% 39 29 0.00% 40 56 0.00% 41 37 0.00% 42 62 0.00% 43 52 0.00% 44 58 0.00% 45 74 0.00% 46 63 0.00% 47 70 0.00% 48 94 0.00% 49 120 0.00% 50 111 0.00% 51 157 0.00% 52 162 0.00% 53 196 0.00% 54 216 0.00% 55 246 0.00% 56 227 0.00% 57 307 0.00% 58 326 0.00% 59 363 0.00% 60 369 0.00% 61 418 0.00% 62 525 0.00% 63 722 0.01% 64 805 0.01% 65 800 0.01% 66 928 0.01% 67 1026 0.01% 68 1152 0.01% 69 1385 0.01% 70 1579 0.01% 71 1866 0.01% 72 2066 0.02% 73 2492 0.02% 74 2784 0.02% 75 3217 0.02% 76 3610 0.03% 77 4055 0.03% 78 4449 0.03% 79 4947 0.04% 80 5572 0.04% 81 6299 0.05% 82 7319 0.05% 83 8428 0.06% 84 9575 0.07% 85 10695 0.08% 86 11859 0.09% 87 12329 0.09% 88 13387 0.10% 89 14429 0.11% 90 15586 0.12% 91 17020 0.13% 92 18806 0.14% 93 20708 0.15% 94 21820 0.16% 95 24085 0.18% 96 25818 0.19% 97 27047 0.20% 98 28118 0.21% 99 29400 0.22% 100 31652 0.24% 101 32942 0.24% 102 35144 0.26% 103 36417 0.27% 104 39115 0.29% 105 40843 0.30% 106 43165 0.32% 107 44924 0.33% 108 45864 0.34% 109 46694 0.35% 110 47978 0.36% 111 48556 0.36% 112 50953 0.38% 113 52886 0.39% 114 54347 0.40% 115 56266 0.42% 116 57915 0.43% 117 59539 0.44% 118 60511 0.45% 119 61248 0.45% 120 61115 0.45% 121 63019 0.47% 122 64617 0.48% 123 65814 0.49% 124 66955 0.50% 125 68019 0.51% 126 70556 0.52% 127 71636 0.53% 128 71877 0.53% 129 72716 0.54% 130 72690 0.54% 131 73522 0.55% 132 74450 0.55% 133 74494 0.55% 134 76647 0.57% 135 76728 0.57% 136 78085 0.58% 137 78582 0.58% 138 78771 0.59% 139 80169 0.60% 140 79163 0.59% 141 80987 0.60% 142 81781 0.61% 143 81687 0.61% 144 82169 0.61% 145 82794 0.61% 146 82666 0.61% 147 83217 0.62% 148 82508 0.61% 149 83341 0.62% 150 83898 0.62% 151 9801174 72.80% 13463750 reads passed initial QC criterion=sequence-density sequence-density=0.37 sequence-density-rank=1 fanout-score=3.90 fanout-score-rank=20 prefix-density=0.47 prefix-fanout=3.0 sequence=TCTCATCACTCACAAGCAAGTCGTGGCGTAGGAATTGAATTAATGAACTTGGTGGCAGCAAGGAAGGCGTTGTAGGAGGCTAA criterion=fanout-score sequence-density=0.03 sequence-density-rank=33 fanout-score=64.88 fanout-score-rank=1 prefix-density=0.28 prefix-fanout=6.4 sequence=TTATTTATTTAAGCAAAGTACCCAGCAACGCCACACATATGAAAAACAAGTAAGAGACTGGCCATGACATGGTTGCTGCATTTTTCTTTTGCGCGAGTTGCATTATTTCACTAACAAGGCTTGGCGCAAAAACAGCGGCGGCGGAACCCTTTGCATTCACCACCGGGAAAACCTTCAGTCTTGCACACACTAGCACAGTTGTGGCCTCTTACACATGGTCCTTTAAAACTATGGCTCTGTGACAAACAAACCCTAGCCTCAGCAGGTACCATCATTTCCTGGGAAGCCAGGGCAATGAGCAGCAACAAGAAAAGACCATAGCATTTCTTCTCCATCTCTCTGTCTAACAAGAACCTGTCTAACACTAGCTCTCTGTCTGCAACTACTACTAGTATC criterion=sequence-density sequence-density=0.34 sequence-density-rank=1 fanout-score=2.07 fanout-score-rank=30 prefix-density=0.35 prefix-fanout=2.0 sequence=TTAGCCTCCTACAACGCCTTCCTTGCTGCCACCAAGTTCATTAATTCAATTCCTACGCCACGACTTGCTTGTGAGTGATGAGA criterion=fanout-score sequence-density=0.09 sequence-density-rank=20 fanout-score=305.47 fanout-score-rank=1 prefix-density=1.07 prefix-fanout=24.9 sequence=AAGAAGAAGAATGAAGATGGCCATAGCAGCAGCAGTGACAGCGACTAAAAATCTTGCACTGCTTCCATGCATTAGGTGTGGAGGAGGTCGAGGTCCTGTCTACCAGTGTTGCTGATTATCACTATAAAAAAAAGAAAGAAAA SRR26075396 testing PE reads STAR mapping to Ensembl genome Started job on | Feb 12 00:34:42 Started mapping on | Feb 12 00:34:42 Finished on | Feb 12 00:41:51 Mapping speed, Million of reads per hour | 112.98 Number of input reads | 13463750 Average input read length | 287 UNIQUE READS: Uniquely mapped reads number | 11000655 Uniquely mapped reads % | 81.71% Average mapped length | 285.97 Number of splices: Total | 7601488 Number of splices: Annotated (sjdb) | 7377119 Number of splices: GT/AG | 7462112 Number of splices: GC/AG | 99037 Number of splices: AT/AC | 9594 Number of splices: Non-canonical | 30745 Mismatch rate per base, % | 0.43% Deletion rate per base | 0.04% Deletion average length | 2.85 Insertion rate per base | 0.03% Insertion average length | 2.59 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 397309 % of reads mapped to multiple loci | 2.95% Number of reads mapped to too many loci | 443013 % of reads mapped to too many loci | 3.29% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 11.24% % of reads unmapped: other | 0.81% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 2065786 2065786 2065786 N_multimapping 397309 397309 397309 N_noFeature 352303 10839814 438444 N_ambiguous 142798 1269 67349 UnstrandedReadsAssigned:10505554 PositiveStrandReadsAssigned:159572 NegativeStrandReadsAssigned:10494862 Dataset is classified negative stranded MeadianReadLen=151 20thPercentileLength=138 echo kmer=133 SRR26075396 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,400 [index] number of k-mers: 62,057,036 [index] number of equivalence classes: 130,681 [quant] running in paired-end mode [quant] will process pair 1: SRR26075396-trimmed-pair1.fastq SRR26075396-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 13,463,750 reads, 11,097,395 reads pseudoaligned [quant] estimated average fragment length: 187.39 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,108 rounds 52401 SRR26075396.ke.tsv 34699 SRR26075396.se.tsv 87100 total ==> SRR26075396.ke.tsv <== target_id length eff_length est_counts tpm Potri.005G200100.1.v4.1 2018 1831.61 2132 73.9549 Potri.005G024800.1.v4.1 1035 848.61 4488 336.014 Potri.004G059700.1.v4.1 961 774.61 10 0.820218 Potri.007G009000.2.v4.1 1416 1229.61 0 0 Potri.003G141000.2.v4.1 2943 2756.61 447 10.3026 Potri.016G087400.1.v4.1 270 101.357 1034 648.154 Potri.015G069301.1.v4.1 564 377.876 0 0 Potri.010G195200.1.v4.1 1773 1586.61 25 1.00111 Potri.012G127500.1.v4.1 977 790.61 5049 405.747 ==> SRR26075396.se.tsv <== Potri.001G166300.v4.1 0 Potri.001G448400.v4.1 15 Potri.001G233950.v4.1 0 Potri.001G122700.v4.1 113 Potri.001G212900.v4.1 0 Potri.001G182400.v4.1 0 Potri.001G256600.v4.1 0 Potri.001G040500.v4.1 0 Potri.001G416900.v4.1 0 Potri.001G452600.v4.1 145 SRR26075396 completed mapping pipeline successfully