Starting /dee2/code/volunteer_pipeline.sh SRR26075397 current disk space = 3051232927744 free memory = 1513937168 SRR26075397 SRAfilesize a3868e0ba9bcc4326fe2b2ca57a2bca7 SRR26075397.sra SRR26075397.sra file validated SRR26075397 is paired end SRR26075397 is conventional basespace SRR26075397 read1 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR26075397_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.54275 37.0 37.0 37.0 37.0 37.0 2 36.635 37.0 37.0 37.0 37.0 37.0 3 36.683 37.0 37.0 37.0 37.0 37.0 4 36.6195 37.0 37.0 37.0 37.0 37.0 5 36.6345 37.0 37.0 37.0 37.0 37.0 6 36.623 37.0 37.0 37.0 37.0 37.0 7 36.5095 37.0 37.0 37.0 37.0 37.0 8 36.551 37.0 37.0 37.0 37.0 37.0 9 36.6195 37.0 37.0 37.0 37.0 37.0 10-14 36.584050000000005 37.0 37.0 37.0 37.0 37.0 15-19 36.593399999999995 37.0 37.0 37.0 37.0 37.0 20-24 36.488299999999995 37.0 37.0 37.0 37.0 37.0 25-29 36.459 37.0 37.0 37.0 37.0 37.0 30-34 36.3686 37.0 37.0 37.0 37.0 37.0 35-39 36.34830000000001 37.0 37.0 37.0 37.0 37.0 40-44 36.3428 37.0 37.0 37.0 37.0 37.0 45-49 36.1687 37.0 37.0 37.0 37.0 37.0 50-54 36.1201 37.0 37.0 37.0 37.0 37.0 55-59 36.010799999999996 37.0 37.0 37.0 37.0 37.0 60-64 35.9935 37.0 37.0 37.0 37.0 37.0 65-69 35.884499999999996 37.0 37.0 37.0 37.0 37.0 70-74 35.9501 37.0 37.0 37.0 37.0 37.0 75-79 36.02810000000001 37.0 37.0 37.0 37.0 37.0 80-84 36.0127 37.0 37.0 37.0 37.0 37.0 85-89 35.948100000000004 37.0 37.0 37.0 37.0 37.0 90-94 35.9027 37.0 37.0 37.0 37.0 37.0 95-99 35.912699999999994 37.0 37.0 37.0 37.0 37.0 100-104 35.8652 37.0 37.0 37.0 37.0 37.0 105-109 35.7885 37.0 37.0 37.0 37.0 37.0 110-114 35.7719 37.0 37.0 37.0 37.0 37.0 115-119 35.5615 37.0 37.0 37.0 37.0 37.0 120-124 35.6605 37.0 37.0 37.0 37.0 37.0 125-129 35.470000000000006 37.0 37.0 37.0 37.0 37.0 130-134 35.342999999999996 37.0 37.0 37.0 34.6 37.0 135-139 35.2936 37.0 37.0 37.0 32.2 37.0 140-144 35.101 37.0 37.0 37.0 29.8 37.0 145-149 34.9781 37.0 37.0 37.0 25.0 37.0 150-151 34.94525 37.0 37.0 37.0 25.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.0 1101 2 0.0 1101 3 0.0 1101 4 0.0 1101 5 0.0 1101 6 0.0 1101 7 0.0 1101 8 0.0 1101 9 0.0 1101 10-14 0.0 1101 15-19 0.0 1101 20-24 0.0 1101 25-29 0.0 1101 30-34 0.0 1101 35-39 0.0 1101 40-44 0.0 1101 45-49 0.0 1101 50-54 0.0 1101 55-59 0.0 1101 60-64 0.0 1101 65-69 0.0 1101 70-74 0.0 1101 75-79 0.0 1101 80-84 0.0 1101 85-89 0.0 1101 90-94 0.0 1101 95-99 0.0 1101 100-104 0.0 1101 105-109 0.0 1101 110-114 0.0 1101 115-119 0.0 1101 120-124 0.0 1101 125-129 0.0 1101 130-134 0.0 1101 135-139 0.0 1101 140-144 0.0 1101 145-149 0.0 1101 150-151 0.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 20 2.0 21 1.0 22 3.0 23 1.0 24 3.0 25 8.0 26 9.0 27 20.0 28 27.0 29 22.0 30 37.0 31 39.0 32 67.0 33 107.0 34 161.0 35 427.0 36 2861.0 37 205.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 37.90342757067801 16.28721541155867 8.781586189642232 37.02777082812109 2 20.45 13.100000000000001 35.6 30.85 3 16.75 16.5 30.075000000000003 36.675000000000004 4 21.525 21.675 24.05 32.75 5 23.549999999999997 27.875 24.95 23.625 6 24.099999999999998 28.675 25.55 21.675 7 14.899999999999999 29.65 38.3 17.150000000000002 8 17.299999999999997 27.35 32.300000000000004 23.05 9 18.0 23.95 35.65 22.400000000000002 10-14 19.570978548927446 29.386469323466173 28.16640832041602 22.87614380719036 15-19 19.715 28.744999999999997 27.125 24.415 20-24 19.07 28.005000000000003 28.51 24.415 25-29 20.345 26.605 28.12 24.93 30-34 19.84 27.939999999999998 27.915 24.305 35-39 20.794999999999998 27.445000000000004 27.139999999999997 24.62 40-44 19.77 27.46 27.68 25.09 45-49 20.645 27.425 27.779999999999998 24.15 50-54 20.28 26.200000000000003 27.99 25.53 55-59 19.75 27.884999999999998 27.555000000000003 24.81 60-64 19.735 26.825 28.255000000000003 25.185000000000002 65-69 20.14 27.634999999999998 27.83 24.395 70-74 20.974999999999998 26.875 27.355 24.795 75-79 22.0 27.42 26.87 23.71 80-84 21.365000000000002 27.43 26.419999999999998 24.785 85-89 21.33 26.87 28.375 23.425 90-94 21.64 27.310000000000002 27.02 24.03 95-99 21.625 26.71 27.634999999999998 24.03 100-104 22.225 27.73 26.575 23.47 105-109 22.11 26.924999999999997 27.74 23.225 110-114 22.155 27.21 26.584999999999997 24.05 115-119 22.09 28.67 26.105 23.135 120-124 22.41 26.455000000000002 27.125 24.01 125-129 22.215 27.6 26.275 23.91 130-134 22.465 27.05 25.869999999999997 24.615000000000002 135-139 21.75 27.08 25.385 25.785000000000004 140-144 22.884999999999998 27.74 25.145 24.23 145-149 21.41 27.815 25.5 25.275 150-151 20.849999999999998 25.887500000000003 27.575 25.687500000000004 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 1.0 15 1.5 16 0.5 17 0.0 18 0.5 19 0.5 20 0.5 21 0.5 22 0.5 23 1.5 24 2.5 25 4.0 26 5.5 27 9.0 28 13.0 29 14.0 30 10.0 31 8.5 32 20.5 33 29.5 34 50.0 35 71.0 36 58.5 37 75.0 38 111.0 39 133.5 40 178.5 41 195.5 42 201.5 43 238.0 44 284.5 45 296.0 46 282.0 47 253.0 48 226.0 49 207.5 50 177.5 51 152.0 52 118.0 53 114.0 54 102.0 55 62.5 56 49.0 57 38.0 58 30.0 59 32.5 60 23.0 61 17.5 62 17.5 63 17.0 64 10.5 65 5.5 66 7.5 67 10.0 68 9.0 69 6.5 70 6.0 71 4.5 72 3.0 73 1.5 74 0.5 75 0.0 76 0.0 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.075 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.005 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150-151 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 57.550000000000004 #Duplication Level Percentage of deduplicated Percentage of total 1 61.29452649869679 35.275 2 21.763683753258036 25.05 3 8.992180712423979 15.525 4 3.5186794092093834 8.1 5 1.7376194613379672 5.0 6 1.4769765421372718 5.1 7 0.6081668114682884 2.45 8 0.26064291920069504 1.2 9 0.13032145960034752 0.675 >10 0.2172024326672459 1.625 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGACCATTATCTCGTAT 23 0.575 TruSeq Adapter, Index 4 (97% over 39bp) CTTGTTTAAAATAAGATCCCGCCATCCTCTTTCAGTATTCTCCACCACAG 12 0.3 No Hit GTGACTAAAAACAGTTGTTATGTTGAAGCAACCCTGACACTTCACATCCA 10 0.25 No Hit CCCAAAACCAACACCAAAAACATCTTCAAAACCACAAATAACAAACGTTT 10 0.25 No Hit CCAGTTTCATCACCAACAAGACATTCATTGATTCGAGAAGGGCGTTGAGG 10 0.25 No Hit CTCCTCCTTTGGTTGGTTTCTTGGTCACAGAGAGCTTGGCGCTCTTAGTA 9 0.22499999999999998 No Hit CTTTCTTGTCTGTCCTCCGTGCTAGCTGGGGATCTTTGCTAAGCAATGCT 9 0.22499999999999998 No Hit CTTAAGCAATCAAAGATTCTTGGTGGCTCGCCTTTGAGATTACTGTTTAG 9 0.22499999999999998 No Hit GCCAAAAATTAACTTCAAGAAAACGGTGAGGATGACCACCACAATGGAGA 8 0.2 No Hit GCCTCAGTAAGATCGTTGGGTTTGATGATTGGGACATCACTCTTTGGTGG 8 0.2 No Hit TGTCCCTTTCCTTTCCCTTTTCTCGAGGAGAGTGGAAGAGGTGGGATGCT 8 0.2 No Hit CTCTGCAAATAGTTCGATATTGCCAATGCATTATGGCTCTGACAGATGCT 8 0.2 No Hit GCTGCTGCTGCTGCTGCTGCTGCTGTTGCTGCAGCTGCTGGTGGTGGTGG 8 0.2 No Hit CACCAAGTTCCCCAAGTACAACTATCATTTTAACCTGTGGGATGTTGTTA 8 0.2 No Hit TCCTCCTTGGGTACACAGTTCAATAAGGAGCTCTCTTGTGCTCCAAGCTC 7 0.17500000000000002 No Hit GTGCAGTATAGGAGTCATACTCGTGTGTCGTACTTATTCTCACAATATGA 7 0.17500000000000002 No Hit ACCTGTGTTTCTGTCAAATTTGCTCTGAAGTATATTCAAGCATTCCGCTT 7 0.17500000000000002 No Hit CTTGTTCCCAGTAAAAAAGGCCCTCACAACTCAAGAAGGGACAAGCAGGG 7 0.17500000000000002 No Hit GTCGGAAAAAAAAAGATCAAGATAGGAAGATAAAAACCCTAAAACAGAAA 7 0.17500000000000002 No Hit CGCCGAGGCATGCTCATGGGCATCGATCTTGGCAACTTCTCTGACGAGGT 7 0.17500000000000002 No Hit GTCTCTGTCCTTCCTCCATGATTAGCTGATAAGCTGCAGATACGTGTTCC 7 0.17500000000000002 No Hit GCGTGGATGAGCTTCTATGAAGAAAGTTCCGTGTTAAGAGAAGAGGTGAA 7 0.17500000000000002 No Hit ACCCGTTCAAGTTTTGACCTGGCAGCATCAAGCTCGGCAACAACTTTCGC 7 0.17500000000000002 No Hit CTTGTCCTTCTGCCAATATTCCCTTATATACTGGTCCAAAACCACCTTCA 7 0.17500000000000002 No Hit GCCCCAAATGTTCTGCCCAGAGAGACATTCGGTACCCATATACCTGGCCT 7 0.17500000000000002 No Hit GGGGGATACGAGAAACGGCACGACCAGTACCCCATGATTCGGCTGAGGTC 7 0.17500000000000002 No Hit GCGGCTCTGAAGATGGTTAGCAGCGCGAGGAAGAGATTGAGGATGTCCAG 7 0.17500000000000002 No Hit GGCGTGGTCGTTACCAAGGTGCTCAAACTCAAAGGTGGACTCGAAGTTCT 7 0.17500000000000002 No Hit TTTTTTTTGAAAAAAACAAAGAAAATACATTTAAATTAAAACGGGAAAAT 6 0.15 No Hit CCAAAGTTTGACCAAAAGCTAGCAGAATCAAACTGCAGAATGCATAGACC 6 0.15 No Hit CCGCGGTGTCGGATCTCCTCTCCGGACTGAATTTCCAAATGCATTGCTGC 6 0.15 No Hit GCTCTCTTCTTCTTCCCTCCGCGTCCCTCTCCCTAACTTTTCAAATTAAA 6 0.15 No Hit ATCTCGTAGAACTGGGTTCCCAATTCTCTTGGTAATCAATACATATCCTC 6 0.15 No Hit TGGTCTTGTGAGAGAAATCCAAGGCTAGCTGGCGCAGCTCTTTTGTCAAA 6 0.15 No Hit GAGGCTTCAGAAAATTAAGACATGGAGGTAGCACTCTTGATTATATACTA 6 0.15 No Hit GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGACCATTATCGCGTAT 6 0.15 TruSeq Adapter, Index 4 (97% over 39bp) CTCCAGGTTGAGCCACTTTGACTAAAGCCCAAGTCAACAACTCTCTACTC 6 0.15 No Hit GTCACAATTAGGAACCCATTCAATTAACCCACTGTTTGAAGATAACGGAA 6 0.15 No Hit GTTGCTGGCAATACGGGGCCAGAGCTCAGCAGCCTCCTTTCCGACGGGGC 6 0.15 No Hit CTCCTCTTCATCCTCAGGTGCATCATCATTATTCTTGAGAGACTCGTCAA 6 0.15 No Hit GTTATAGCTGAATTCTTTTTTCAAACCTTGCACAGTTGTCAAGCTTTTCC 6 0.15 No Hit GGCCATATCAGTTTCTAGGCAGTGAGAGTTTGCCCTGGATGTAATGCAAG 6 0.15 No Hit ATTTCCTGTACCAAGGGGAATAATAGCAACAGGTGGTGGAGATTCAAAGT 6 0.15 No Hit CGAGAAATTAATTAATATTCACTACCACAACAGACCCGAAAACAAATCTT 6 0.15 No Hit ATCTAAATACCGATCGAAACATTTAGCCAAAGTGAATCAAAAGAATCCAG 6 0.15 No Hit CTTATAAACGCCTAGTAGATGAAATATTATTTCTTGTCAATCCGTCGATG 6 0.15 No Hit GGAAAAAATAAAGCATGGGCAAAGGTGTTTTGTGAGGTTTAGACAAAAGC 6 0.15 No Hit CTCTAAACTCCAATGAAGTGCTTCTGTATGGACAATTTAGTTGATGATTG 6 0.15 No Hit CCCTACCTCAATAAATTCTCCATATCAACCAGGACTTTGAGTTCATGACA 6 0.15 No Hit CTTGGATGAAGGGGCAAAGAGATCACCAGGTTTATTTCCGTTACCATCAA 6 0.15 No Hit GTAACTCACTATTGACGTTGGCTTAATACCGGCCCCAACAAGGAAATCAA 6 0.15 No Hit GTACCGTCTTGCCCTTGTGGGTATCAGCAAGCTTCACAGTGATAGTTCTC 6 0.15 No Hit ACACCTTAATGACATCACAATCATAATCTGTTCTTCAATAACAATAATCC 6 0.15 No Hit TCTGATTTGTTCACAAAGAGAAGGATTTAAAAGTAGTAATTTCCTTGGCT 6 0.15 No Hit AAGACGGGGACGAGGGATGCAAAGGTCTGGGAGGATATGCCCTCAGCTTC 6 0.15 No Hit CCTACTAGTTAGCTTGCGCATCAGATTCCACCCGTGTACACCTCCCCCTT 6 0.15 No Hit CGGTGAACATAGGACTGAAATTTCTAAGAAGCGAGTGCAATTTACTGGCT 6 0.15 No Hit GCCAGAGTTTACATGAAAATGCTGTTATTTCTCATAAAAACTACTAGTAT 6 0.15 No Hit CTTGCGAGCACGTCTTGGGAAGACAACCAATTTGGCCTTGTATGTTTTCA 6 0.15 No Hit TCTCGCAATAACTTCTCATTCTTTGCATTCTCTTCTTGAAATCGAGCCCA 6 0.15 No Hit GTGAGGAGAGAGTTGAGTGACGGTCGACGACGACGATGCGGGTGTCGCCT 6 0.15 No Hit GGGTCAAGTTCACCATCCTGAAAATTTATTGCCACTGTATCCCTCCAAAC 6 0.15 No Hit ACCGCTCCCATCTGCCCTTGCTTAGCACTTTTCTTGATCTCAGCGATTAG 5 0.125 No Hit TTTCCTTCTTCACAATGCCAGCCTGAACAAGGAAGGTCGATACATTCTTG 5 0.125 No Hit GCAAGTAGCAACCTCTCTTCCAAATGTTGTTTTGAAGGATACTCCGGTAA 5 0.125 No Hit GCATCTTTGACTTGAAAGAATGTATCTTCTTACGCTTGGCAGCTTTCACA 5 0.125 No Hit CCTTTGCTATGCTCTGCCTGATCGCATAGATCTGAGACATGTGTCCTCCA 5 0.125 No Hit ATCCACCATACCCTGGTCCTTGAGAATATTTGAAATGATCATCGGAATTA 5 0.125 No Hit GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGACCATTATCTCGTTT 5 0.125 TruSeq Adapter, Index 4 (97% over 39bp) ACCATGTTGACAAACAGAGGACGTATATATAGAAAGAAAGCAAACAGCAG 5 0.125 No Hit CGTATTTGCTCCTATCAGTCTTGTACATGTGGGCAATCTCAGGCACCAGA 5 0.125 No Hit CAACAGTAGTGCACCATCAATATCTGCAGTTGAAACCCTCTAGTGGAGGA 5 0.125 No Hit GGCCATCTCAATTTGTCTCGTATCTCCAGTTACACGCACTGTTCTTTCTT 5 0.125 No Hit GTACCGTGTTTATAACATACTAACATCCCTAGTTAAAAGGCCATCCTGAA 5 0.125 No Hit AGTAGTAATGCGAGAAAGTCCCTCTTCTTCAGCAGGCCCCACAGCAAGAC 5 0.125 No Hit TGCTTTTCTAAAATAAATGCTTGTCCTTCAAGGTGGCATTTGCCTTTCTT 5 0.125 No Hit AGGGTTTCTAGACTTTAATGAGTATCATCAAGCAGCTGCAGCCATTGGCA 5 0.125 No Hit GGATGCTTTCCATTAGAACTCTCTTGTTCTTGAACACATTACCCTTCACC 5 0.125 No Hit GCTCAAAACTGCATAAAAAATCCCTTGTAAGAAATGGACCAGATCACCCA 5 0.125 No Hit GCAATACACCAAATACTTAATTTGTCCTTTTATTGACTTGGAGTTCTGGC 5 0.125 No Hit CTCTGGTCTATTCATCTTTATGCATGCTACAGTAAGCTGCCAAAGCAAAG 5 0.125 No Hit GTCTTTGCAACTTTCTCCATAAACTCCACCATCTCTTCATAACGTTCGGC 5 0.125 No Hit GGTGGGTATCCTGGTGGTGGATAAGCGTCCTTAGGATATCCTTCTGGTGG 5 0.125 No Hit CTGTGATCAAATAACAGAGCGTGACGCGACCAAACCCATAGCCACCACCA 5 0.125 No Hit AAGTTGGTTCATTCCACCTTTAGTAGCTGAATATATAGGATATTGAGGGT 5 0.125 No Hit GTGAGGAGAGCGAGGATGATGAGGATGAGAGTGAGACTTCGGATTCTGAG 5 0.125 No Hit AGCGTCCTTCGTGGCCTGCCTTTGAGAGTCATTGAAGTAAGCAGGGACAG 5 0.125 No Hit CGGCAGGGTAAAGGGCCTGAATAGGTGTCCAGCCGAACGAGAAGAAGACC 5 0.125 No Hit GGTGATTTACAAAGTTACAGCTTCGAAGGTTTCTTGGAGCTCCGACCAGC 5 0.125 No Hit CCACAGTAGTGCCTATCAAAATGATTAGCCATGAAAGTTCCAGCACCGCA 5 0.125 No Hit TTGCCAGTGAAACATATCTTCAGCTACAGGACCTGCACTGCAAGAAGTAG 5 0.125 No Hit ACGTGAACAGGTTCTCCATGCATCCCTTCACTTTGAGAATCATCCCTGGC 5 0.125 No Hit CTAAATCTTAATACTTAAGAGTGATGTGATATCATATCCTAGTGTTCTTG 5 0.125 No Hit CTCCTGTTCTGAAACCACACCTCCACTTGTCTAGGTCTGAGTCCTAACTG 5 0.125 No Hit ACCTGCAGTATCACGAAGCATGCCTCTTTGTCTGAGAATGCCACATAGAT 5 0.125 No Hit CAGTGCTGTGAATCCACTAATGCATTGAGCTACATCTCCCTCGCCAACTT 5 0.125 No Hit CTCCTTTACACTTTTTGTCTTCCCATGCAGTTGGATCAATGTTCGAACCC 5 0.125 No Hit CGCACTCTCCAAGTCCCGTAAATGCAGAGTATATCTTATTCTCGGTAATT 5 0.125 No Hit CGGTATGGGTGCTCGGGGATTTGCTGGTGCTCACTGGCATTCCATAAGGC 5 0.125 No Hit GGTCTCTTTCCAATCAATACGGGTGTTAACAAAAGCTGGATTTTCACGAG 5 0.125 No Hit GCTCACACATACTCCACCAATAGCACCTCCCAGTACAGAATAGCAAATGA 5 0.125 No Hit GTTTTCTTGGTAGAATCTTTCTTTATAACCTTGCTTGGGTACTTCTTGAT 5 0.125 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0 0.0 0.0 0.0 0.0 64-65 0.025 0.0 0.0 0.0 0.0 66-67 0.025 0.0 0.0 0.0 0.0 68-69 0.037500000000000006 0.0 0.0 0.0 0.0 70-71 0.05 0.0 0.0 0.0 0.0 72-73 0.05 0.0 0.0 0.0 0.0 74-75 0.05 0.0 0.0 0.0 0.0 76-77 0.0875 0.0 0.0 0.0 0.0 78-79 0.1375 0.0 0.0 0.0 0.0 80-81 0.15 0.0 0.0 0.0 0.0 82-83 0.2 0.0 0.0 0.0 0.0 84-85 0.225 0.0 0.0 0.0 0.0 86-87 0.25 0.0 0.0 0.0 0.0 88-89 0.3 0.0 0.0 0.0 0.0 90-91 0.5125 0.0 0.0 0.0 0.0 92-93 0.6 0.0 0.0 0.0 0.0 94-95 0.6625 0.0 0.0 0.0 0.0 96-97 0.9624999999999999 0.0 0.0 0.0 0.0 98-99 1.1375000000000002 0.0 0.0 0.0 0.0 100-101 1.4500000000000002 0.0 0.0 0.0 0.0 102-103 1.7125 0.0 0.0 0.0 0.0 104-105 2.2625 0.0 0.0 0.0 0.0 106-107 2.7125 0.0 0.0 0.0 0.0 108-109 3.3625 0.0 0.0 0.0 0.0 110-111 3.7375 0.0 0.0 0.0 0.0 112-113 4.4625 0.0 0.0 0.0 0.0 114-115 5.0375 0.0 0.0 0.0 0.0 116-117 5.4125 0.0 0.0 0.0 0.0 118-119 5.95 0.0 0.0 0.0 0.0 120-121 6.362500000000001 0.0 0.0 0.0 0.0 122-123 6.925 0.0 0.0 0.0 0.0 124-125 7.7625 0.075 0.0 0.0 0.0 126-127 8.325 0.075 0.0 0.0 0.0 128-129 8.875 0.075 0.0 0.0 0.0 130-131 9.5125 0.075 0.0 0.0 0.0 132-133 10.55 0.075 0.0 0.0 0.0 134-135 11.6375 0.075 0.0 0.0 0.0 136-137 13.0125 0.075 0.0 0.0 0.0 138-139 13.675 0.075 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GAGGCAT 10 0.006830828 145.0 5 GGCATGC 10 0.006830828 145.0 7 CGAGGCA 10 0.006830828 145.0 4 CCGAGGC 10 0.006830828 145.0 3 ACAAGAA 10 0.006830828 145.0 5 GCCGAGG 10 0.006830828 145.0 2 CGCCGAG 10 0.006830828 145.0 1 >>END_MODULE SRR26075397 read2 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR26075397_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.2985 37.0 37.0 37.0 37.0 37.0 2 36.254 37.0 37.0 37.0 37.0 37.0 3 36.2195 37.0 37.0 37.0 37.0 37.0 4 36.321 37.0 37.0 37.0 37.0 37.0 5 36.273 37.0 37.0 37.0 37.0 37.0 6 36.0975 37.0 37.0 37.0 37.0 37.0 7 36.141 37.0 37.0 37.0 37.0 37.0 8 36.2455 37.0 37.0 37.0 37.0 37.0 9 36.1795 37.0 37.0 37.0 37.0 37.0 10-14 36.0303 37.0 37.0 37.0 37.0 37.0 15-19 35.98440000000001 37.0 37.0 37.0 37.0 37.0 20-24 35.8906 37.0 37.0 37.0 37.0 37.0 25-29 35.7382 37.0 37.0 37.0 37.0 37.0 30-34 35.6413 37.0 37.0 37.0 37.0 37.0 35-39 35.529199999999996 37.0 37.0 37.0 37.0 37.0 40-44 35.477199999999996 37.0 37.0 37.0 37.0 37.0 45-49 35.4159 37.0 37.0 37.0 37.0 37.0 50-54 35.1452 37.0 37.0 37.0 37.0 37.0 55-59 35.266999999999996 37.0 37.0 37.0 37.0 37.0 60-64 35.368900000000004 37.0 37.0 37.0 37.0 37.0 65-69 35.17530000000001 37.0 37.0 37.0 37.0 37.0 70-74 35.1395 37.0 37.0 37.0 34.6 37.0 75-79 35.0513 37.0 37.0 37.0 29.8 37.0 80-84 35.0728 37.0 37.0 37.0 34.6 37.0 85-89 35.120799999999996 37.0 37.0 37.0 34.6 37.0 90-94 35.029399999999995 37.0 37.0 37.0 25.0 37.0 95-99 35.0663 37.0 37.0 37.0 29.8 37.0 100-104 35.0111 37.0 37.0 37.0 29.8 37.0 105-109 34.9516 37.0 37.0 37.0 29.8 37.0 110-114 34.9272 37.0 37.0 37.0 27.4 37.0 115-119 34.9842 37.0 37.0 37.0 25.0 37.0 120-124 34.8645 37.0 37.0 37.0 25.0 37.0 125-129 34.8584 37.0 37.0 37.0 25.0 37.0 130-134 34.762299999999996 37.0 37.0 37.0 25.0 37.0 135-139 34.7622 37.0 37.0 37.0 25.0 37.0 140-144 34.626099999999994 37.0 37.0 37.0 25.0 37.0 145-149 34.65885 37.0 37.0 37.0 25.0 37.0 150-151 34.182874999999996 37.0 37.0 37.0 25.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.0 1101 2 0.0 1101 3 0.0 1101 4 0.0 1101 5 0.0 1101 6 0.0 1101 7 0.0 1101 8 0.0 1101 9 0.0 1101 10-14 0.0 1101 15-19 0.0 1101 20-24 0.0 1101 25-29 0.0 1101 30-34 0.0 1101 35-39 0.0 1101 40-44 0.0 1101 45-49 0.0 1101 50-54 0.0 1101 55-59 0.0 1101 60-64 0.0 1101 65-69 0.0 1101 70-74 0.0 1101 75-79 0.0 1101 80-84 0.0 1101 85-89 0.0 1101 90-94 0.0 1101 95-99 0.0 1101 100-104 0.0 1101 105-109 0.0 1101 110-114 0.0 1101 115-119 0.0 1101 120-124 0.0 1101 125-129 0.0 1101 130-134 0.0 1101 135-139 0.0 1101 140-144 0.0 1101 145-149 0.0 1101 150-151 0.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 13 2.0 14 16.0 15 9.0 16 14.0 17 9.0 18 11.0 19 11.0 20 16.0 21 17.0 22 24.0 23 13.0 24 23.0 25 20.0 26 30.0 27 33.0 28 20.0 29 12.0 30 19.0 31 30.0 32 50.0 33 66.0 34 163.0 35 575.0 36 2610.0 37 207.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 41.8 23.7 11.975 22.525000000000002 2 33.025 23.7 25.624999999999996 17.65 3 26.650000000000002 24.85 28.175 20.325 4 28.175 33.175 20.175 18.475 5 28.95 34.875 20.125 16.05 6 23.575 37.574999999999996 21.925 16.925 7 25.15 22.025 34.35 18.475 8 24.65 25.650000000000002 26.5 23.200000000000003 9 27.325 24.349999999999998 25.825 22.5 10-14 27.384999999999998 27.224999999999998 24.735 20.655 15-19 26.055 27.700000000000003 26.235000000000003 20.01 20-24 26.490000000000002 28.285 25.61 19.615 25-29 26.39 26.974999999999998 25.885 20.75 30-34 25.729999999999997 27.98 25.4 20.89 35-39 25.515 28.720000000000002 25.28 20.485 40-44 26.334999999999997 27.615000000000002 25.7 20.349999999999998 45-49 25.82 28.13 26.5 19.55 50-54 24.9 26.875 26.755000000000003 21.47 55-59 25.040000000000003 28.055000000000003 26.435 20.47 60-64 25.990000000000002 28.18 26.169999999999998 19.66 65-69 26.435 27.955000000000002 25.765 19.845 70-74 25.619999999999997 28.88 25.45 20.05 75-79 24.67 29.065 25.595000000000002 20.669999999999998 80-84 25.77 27.955000000000002 25.645 20.630000000000003 85-89 26.11 28.105000000000004 25.245 20.54 90-94 26.255 28.449999999999996 25.805 19.49 95-99 26.255 28.335 26.22 19.189999999999998 100-104 26.41 28.975 24.915000000000003 19.7 105-109 26.095000000000002 29.265 25.14 19.5 110-114 26.14 28.515 25.615 19.73 115-119 26.66 28.535 25.540000000000003 19.265 120-124 27.11 27.71 26.245 18.935 125-129 27.025 27.884999999999998 26.3 18.790000000000003 130-134 27.935 29.185 24.855 18.025 135-139 26.845000000000002 28.544999999999998 24.795 19.814999999999998 140-144 27.507750775077504 29.752975297529755 24.782478247824784 17.956795679567957 145-149 28.284242636395458 28.029204380657095 24.418662799419913 19.267890183527527 150-151 29.141142642830353 27.565945743217902 25.115639454931866 18.17727215901988 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.5 6 1.5 7 1.0 8 1.5 9 2.5 10 1.0 11 0.5 12 2.0 13 2.0 14 1.5 15 1.0 16 0.5 17 3.0 18 3.0 19 1.5 20 2.0 21 2.0 22 1.5 23 3.0 24 5.5 25 4.0 26 3.5 27 5.5 28 4.5 29 8.0 30 12.5 31 17.5 32 16.5 33 23.0 34 25.5 35 25.5 36 53.5 37 68.0 38 88.5 39 122.5 40 152.5 41 197.0 42 238.5 43 282.0 44 292.0 45 295.5 46 297.5 47 254.0 48 219.0 49 201.0 50 171.5 51 141.5 52 119.5 53 103.0 54 84.5 55 64.0 56 49.5 57 39.5 58 34.5 59 24.5 60 21.0 61 16.0 62 13.0 63 11.5 64 8.5 65 11.5 66 8.0 67 4.0 68 3.0 69 3.0 70 3.5 71 2.5 72 1.5 73 3.5 74 3.5 75 1.0 76 1.5 77 1.5 78 1.0 79 3.0 80 4.5 81 2.5 82 3.5 83 6.0 84 6.0 85 5.0 86 6.0 87 8.0 88 7.0 89 5.0 90 3.0 91 2.0 92 1.5 93 1.5 94 1.5 95 0.5 96 0.0 97 1.5 98 2.0 99 2.0 100 17.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.01 145-149 0.015 150-151 0.0125 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 58.4 #Duplication Level Percentage of deduplicated Percentage of total 1 63.6986301369863 37.2 2 20.11986301369863 23.5 3 8.732876712328768 15.299999999999999 4 3.3818493150684934 7.9 5 1.8407534246575343 5.375 6 1.1558219178082192 4.05 7 0.3852739726027397 1.575 8 0.3424657534246575 1.6 9 0.08561643835616438 0.44999999999999996 >10 0.21404109589041095 1.675 >50 0.04280821917808219 1.375 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG 55 1.375 No Hit GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGT 25 0.625 No Hit AGTGAAGGTGATGCATACTGATATACCCGAGGAAGACAGATATGGGAATG 12 0.3 No Hit GTTTTGACGCTTGTGAATTGGTGAGTCAGTTATTGCCTGTTGTTGAAGGT 10 0.25 No Hit AAGCAAGTGTCTGGGGTACCAAGTACTTCAAGAACAACTTCAACAGGTTG 10 0.25 No Hit AATGGTCACAATCTCACTGTCAAAGTTCTTGAATCTAAACCAGTCCCTGT 10 0.25 No Hit AGAGTTTCAAGAGAGGGAGACATGCCCAACTTCTTCCCTACAGCTTCCTC 9 0.22499999999999998 No Hit CTGCGAAAGAAAGAAAGAAGGAAAGAAGCTGTTTCAAAGTTCAAAATGGT 9 0.22499999999999998 No Hit GATGACATTATACAGAATCGTGTGTTAGAAGCATTGTATCAGCGTATTAT 8 0.2 No Hit AGAAGAAAAGATTAAAAAAAAAAAATCAAGAAAAGGAATTTTGATTAAAT 8 0.2 No Hit GTTGGATTTGTCTCCAAATCAGGTGGTATGTCTAATGAACTATACAATAG 8 0.2 No Hit TGATGATGGCTACTTTAAAATCAGAAGAGGAACAAATGAGTGTGGTATTG 8 0.2 No Hit GCTTTCCTCTCCAAAATTCACTTTCTGCTATGATGCACTACATGGAGTTG 8 0.2 No Hit GAAGAAGGTTTTTGAAATCCTTTACCAGGTTGCAGAGATTTCTTTCTTAA 8 0.2 No Hit TCTGCTGCTACAAGAGGGCATACTGCAGTAGTCATTGAATTGCTGTCAAA 8 0.2 No Hit AGCTGATACTGTGGAAGGAGTGGAAATTAAGCTATGGCAGGTACCTGAAA 8 0.2 No Hit TGAGTCTTCTACCCACTCTTCAGGGAATAACATGACACAAAGCTGGATCG 7 0.17500000000000002 No Hit GATAACTTCAAGGATGTAAGGAAAGAAGACATGGAGTTTCCGATATTTTA 7 0.17500000000000002 No Hit ATATGACACCGATAACCTAATCAAGCGTTTCAGTTATGATGAATACATAT 7 0.17500000000000002 No Hit ATGAGATCTGACGTTTGGAATCTCATGGAAAAAACTTGACAAAGTTGATA 7 0.17500000000000002 No Hit ACTTGGCGGGGAATACCACGGAAGCCAAAGAATGGAAGGGAAACAACCTG 7 0.17500000000000002 No Hit AGGAAACTTCTTCAAAAGCAAATCATGGGTTCAAAGAAATCCAATAAGAA 7 0.17500000000000002 No Hit CTGGATGCCATTTTCAGAAATCCGACGGATCTACATGAAGGATCTTCAGA 7 0.17500000000000002 No Hit AATACTGGGATCTGCCAATATTAACGAACGATCTATGGCCGGTTCAAGAG 7 0.17500000000000002 No Hit AGAGCAAGAAGCAGCTGTGATGGAATTGCAAAACCGTTTGAAAATAAACA 7 0.17500000000000002 No Hit GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGT 6 0.15 No Hit ATCTAATGCATAGAAAAGCGGAAGTGAAGGATATCATTACAGATGTGATA 6 0.15 No Hit TGAAGATAAGAAAGAGGAGAAGCCCGATCCCTCAACTTGAGGGTTGATTG 6 0.15 No Hit TTTTGATGGGGATTTTAGTGATGATGAGGAGGAGGATTTGAAGAAAATTG 6 0.15 No Hit CCGCAACACCGATTCCAACTACTTCGACGACCCACTGCCCCCTCTCTCTT 6 0.15 No Hit CGCCTATTAAAGAGCTACCCTTATCGATCAAAGATATGGTATGTTTGGAT 6 0.15 No Hit GTCATGAACTGCTGTGACAACTCCGGTGCCCGAAACCTCTACATCATCGC 6 0.15 No Hit GATTAAATATGGTTTGGTTGTGTTATTTAATGTGACAATGTGGGGATGTT 6 0.15 No Hit TGTGGACATTGTCTCTGGCACACGAGTCAAGTCTCACTTCGCCAAGCCTG 6 0.15 No Hit CAGGTTCAAACCCTTGAATTCTCCGTTCGAGACCAACCAAAACAACAGCG 6 0.15 No Hit GACGAGATGATTGAGTGAATTGCTGGTGGTTTCTGAAGTCGGCTCTATTT 6 0.15 No Hit GTGAGATTGAAGAAGGCTCAGTTGCCTATAACAAAGATACTAATGAATCA 6 0.15 No Hit AGCACCTTCCAGCAGATGTGGATTTCCAAGGGTGAGTATGATGAGTCTGG 6 0.15 No Hit AACACCAACATCTTTCTCTTCAAGTCTCTACTCTAATTGCTATATGAGCA 6 0.15 No Hit CTTTTGTTCCAGGACTTGTTGATTTGGCTATTAAGAGGAACAGTTTGATT 6 0.15 No Hit AGGGACGTCGTGGTGCCGAATATGTTCCTGCAAGTAAAGCAGGTGGTACT 6 0.15 No Hit AGAGAAGGGTCACTTTGCGGGAGCTGCTGAGACGGTCGTGGGAGCCGAGA 6 0.15 No Hit TTAAGTTTGTTTGGCTCCTTTTCTCATGGTGGTCTTTCATGGCAAGAAGG 6 0.15 No Hit AGAGAATCTAGAAGGGAGAAAAAGGCTGAAACAGCTGCTGAACTGGATAA 6 0.15 No Hit GGTCAACTTGGGCCTATTCTTCGAAGGACACTGAACATGCTGTTGAATCC 6 0.15 No Hit GCCAGACTTTGAAGTATAACATGAAAGTGAGAGCTGGTAGGGGATTTTCT 6 0.15 No Hit AGCTTAGACTCATCCGATTTAGGGTTTCGCTTCATATCAATCAAATAATC 6 0.15 No Hit CAGGTACTGGTCGGCCTATTCCGGTTCAAATCTCTATCAGGTCACCAAAC 6 0.15 No Hit CGTCAGTTGGGGTTGTGAAGCTCTTGCTTGATGCATCTGCTGATCCTAAC 6 0.15 No Hit GAATCTTTCGGCAGTTGTAAACTCTTCAACTTTTTAAATCACAATCACTC 6 0.15 No Hit GGGACATGAAGATTTGCGCCAAGACGAACGTGTCATGCAGCTATTTGGAT 6 0.15 No Hit GGCTGTTCAATGTGCTCATAGTCAAATCCGATTGGGTTTCCCCGACAGGG 6 0.15 No Hit GAAACAGCAATTATAGTGGAAGGGAGAACAGTAGGGAAGGAAGACGCAAT 5 0.125 No Hit TATCTCTCCGTAAAAATCTAAGCTTTGATCCGTTCTCTCTCTTTGTCCCT 5 0.125 No Hit GCATCTAAGGGCACGGAATTGGAATGTCAAGAAGGCACTGAAAATGCTGA 5 0.125 No Hit GGAAGTAGTTTTATGTCTGAAGTGCTGGATGGCCCTGATGATGGATGCCG 5 0.125 No Hit GTTGGTCCACTGTCGACTTATTTCCAGATCGCAGATTTCCCCTTCCATCA 5 0.125 No Hit TGGAGCTTGGACTTTCACAGGATGGGCATGGTGGGATGTTCAATCCTCAA 5 0.125 No Hit GTGAAAATGCGGAATAAGAAGACCGTGTCACCGGTCCAGGCAATTCATCC 5 0.125 No Hit TGAATATAAACCTTGGATCTGCTTTCCATCTGTCCCAACTTGCACATCCA 5 0.125 No Hit CATCCAAGGTGCCTTTGGAAGGCTTTAGTGCTCTCCAAGGAATCTCAGGT 5 0.125 No Hit CAGAGTACTACTCTTCTTCTTACCATACCTTTCAGCAATAATCCTCTCAA 5 0.125 No Hit CCTGATAGTCCGTATGCAGGAGGAGTCTTTCTAGTCACAATTCATTTTCC 5 0.125 No Hit TTGGATTCAGGGCGATAACATGTATGCTTCCTGTGATTCACGGCATTATG 5 0.125 No Hit ACCAACTACTCCTCTTTTCTTATATAAAAACAGCCCACTGACAACCTAAC 5 0.125 No Hit GTCTTTTGGTGGTGGCTATGGCTATAACTCCTTGAGCCAGAGTGTTTCTT 5 0.125 No Hit CTGAAATCCCAGAGGATCTCTACATGTTGATCAAGAAGGCCGTTTCCGTC 5 0.125 No Hit TGGGCGCAGAATTTGCCTTCAAGTTCTTCCTCCTTTATCTTCTGCTTGTT 5 0.125 No Hit GGTCGTTGCTGGTCCTGGTGACAAGCCTATGATTGTGGTTACCTACAAGG 5 0.125 No Hit AAGTTCCCCATCGGTTCAAAAGTTCAAGCTGTATGGAGTGAAGATGGGGA 5 0.125 No Hit ATAAGTTTAATATCTATCCAGATCAGATTCCTCCCTGGCTGGTTGAATTC 5 0.125 No Hit CCTTTGGCCAGCTCTCCGGAAACGGCCTTATTACATACTTCCTTCCCGTC 5 0.125 No Hit GAGCATCAAAATTCTCCAGTTTCCTGCTCCTGCTGACAGGGATGTCGATC 5 0.125 No Hit CGGCAACACAGAGAGTAAAGGAGAGAGGGAGAGAAAACCCTAAGCCGCCG 5 0.125 No Hit CTGATAGCTATGGGCCTTGCTACTAGTGCACAAGCTGCTGAAGTGGGAGA 5 0.125 No Hit AGGCAATCAAGAGTTCCACAGCTAGTCATCAAACCAAAAGATTGTATCCG 5 0.125 No Hit TCTCGGTCCCGCGCTCAACGCATGAAGGAAGCTAAGAGAAAGGGACGTCA 5 0.125 No Hit CTTAATGGCAGCAACTCTTCATCCTCAAAGGGTGATGTCTATCCTGTGGA 5 0.125 No Hit CCTGAAGTTGGACCTGGGTCATGCTCTTTCTTTAGAAAAGGAGAGGGCTC 5 0.125 No Hit CGCATTTTGTTAAGACCTAGAGCGAAGAAGCTTGGATTAGGCACGGCTTA 5 0.125 No Hit ATAGACTATAGGATATCTTCTATTTTCAAAAAAAAAAGAAAAAAGATTGG 5 0.125 No Hit ATTTTGACAAGAGTGTGGAATATTGGCAACAAGACAAGTGGACAGGCTAT 5 0.125 No Hit GTTTATATGCTGTGATTGTGATGAACAAGGTTCAGGATGGGCTTATCAGT 5 0.125 No Hit TGGCTGTTCCACAGGTTGAAGATATTGTTCGTGCAGCAAAGAGAGCTTGC 5 0.125 No Hit AGAGAGCGAGAGAGAGATCCGTCTCTGCAAATCTCTTTCCACGAAGTAAC 5 0.125 No Hit TGACAACTGTGCAAGGTTTGAAAAAAGAATTCAGCTATAACAAGATACTA 5 0.125 No Hit GAAGATACAATGCTTTGGATGCACTGCGCTTGATTTCTCTAGTTGATGAA 5 0.125 No Hit GTGAATGAGCATGCTCAGAAGGCATCAGATGGTTTTCATGATGCAATGTG 5 0.125 No Hit AGGGTGCCTTTCCTCGGTTTTTGGCTATGCAGGAGGCAATTAATCAGCTA 5 0.125 No Hit TGGAAATGGTGGCTTTTGACTTTTCTATATCTTTGATTTTTTCTCTTTGG 5 0.125 No Hit GCCACAGAGACGCTTCCAAGGAACGAGGTGCTTGGAGGATATATCTTTGT 5 0.125 No Hit AGAAGATGGTGAAACCTCAAGAAAGAAGCTCAGGCTGTCTAAAGATCAGG 5 0.125 No Hit GCCTGAGAAACGGCTACCACATCCAAGGAAGGCAGCAGGCGCGCAAATTA 5 0.125 No Hit AGAAGGTCAAGCTCGCTGTTCTCCAGTTCTATAAGGTTGATGATAGCGGC 5 0.125 No Hit AGATCCGGCTGGCAAAGGAGGCCACCGACCATGCCGTCTACATTGATACC 5 0.125 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0 0.0 0.0 0.0 0.0 64-65 0.025 0.0 0.0 0.0 0.0 66-67 0.025 0.0 0.0 0.0 0.0 68-69 0.037500000000000006 0.0 0.0 0.0 0.0 70-71 0.05 0.0 0.0 0.0 0.0 72-73 0.05 0.0 0.0 0.0 0.0 74-75 0.05 0.0 0.0 0.0 0.0 76-77 0.0875 0.0 0.0 0.0 0.0 78-79 0.1375 0.0 0.0 0.0 0.0 80-81 0.15 0.0 0.0 0.0 0.0 82-83 0.2 0.0 0.0 0.0 0.0 84-85 0.225 0.0 0.0 0.0 0.0 86-87 0.25 0.0 0.0 0.0 0.0 88-89 0.3 0.0 0.0 0.0 0.0 90-91 0.5125 0.0 0.0 0.0 0.0 92-93 0.6 0.0 0.0 0.0 0.0 94-95 0.675 0.0 0.0 0.0 0.0 96-97 0.9875 0.0 0.0 0.0 0.0 98-99 1.1625 0.0 0.0 0.0 0.0 100-101 1.4875 0.0 0.0 0.0 0.0 102-103 1.775 0.0 0.0 0.0 0.0 104-105 2.3375 0.0 0.0 0.0 0.0 106-107 2.75 0.0 0.0 0.0 0.0 108-109 3.4625000000000004 0.0 0.0 0.0 0.0 110-111 3.8 0.0 0.0 0.0 0.0 112-113 4.5125 0.0 0.0 0.0 0.0 114-115 5.1125 0.0 0.0 0.0 0.0 116-117 5.4875 0.0 0.0 0.0 0.0 118-119 6.0375 0.0 0.0 0.0 0.0 120-121 6.425000000000001 0.0 0.0 0.0 0.0 122-123 6.975 0.0 0.0 0.0 0.0 124-125 7.800000000000001 0.0 0.0 0.0 0.0 126-127 8.375 0.0 0.0 0.0 0.0 128-129 8.9875 0.0 0.0 0.0 0.0 130-131 9.649999999999999 0.0 0.0 0.0 0.0 132-133 10.7125 0.0 0.0 0.0 0.0 134-135 11.7 0.0 0.0 0.0 0.0 136-137 13.075 0.0 0.0 0.0 0.0 138-139 13.75 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGCTGAT 10 0.006830828 145.0 9 GCTACTT 10 0.006830828 145.0 9 GGCTACT 10 0.006830828 145.0 8 GTGGAAC 10 0.006830828 145.0 6 TGGCTAC 10 0.006830828 145.0 7 >>END_MODULE Read 910235 spots for SRR26075397.sra Written 910235 spots for SRR26075397.sra Read 910235 spots for SRR26075397.sra Written 910235 spots for SRR26075397.sra Read 910235 spots for SRR26075397.sra Written 910235 spots for SRR26075397.sra Read 910235 spots for SRR26075397.sra Written 910235 spots for SRR26075397.sra Read 910235 spots for SRR26075397.sra Written 910235 spots for SRR26075397.sra Read 910235 spots for SRR26075397.sra Written 910235 spots for SRR26075397.sra Read 910235 spots for SRR26075397.sra Written 910235 spots for SRR26075397.sra Read 910235 spots for SRR26075397.sra Written 910235 spots for SRR26075397.sra Read 910235 spots for SRR26075397.sra Written 910235 spots for SRR26075397.sra Read 910235 spots for SRR26075397.sra Written 910235 spots for SRR26075397.sra Read 910235 spots for SRR26075397.sra Written 910235 spots for SRR26075397.sra Read 910235 spots for SRR26075397.sra Written 910235 spots for SRR26075397.sra Read 910235 spots for SRR26075397.sra Written 910235 spots for SRR26075397.sra Read 910251 spots for SRR26075397.sra Written 910251 spots for SRR26075397.sra Read 910235 spots for SRR26075397.sra Written 910235 spots for SRR26075397.sra Read 910235 spots for SRR26075397.sra Written 910235 spots for SRR26075397.sra Read 910235 spots for SRR26075397.sra Written 910235 spots for SRR26075397.sra Read 910235 spots for SRR26075397.sra Written 910235 spots for SRR26075397.sra Read 910235 spots for SRR26075397.sra Written 910235 spots for SRR26075397.sra Read 910235 spots for SRR26075397.sra Written 910235 spots for SRR26075397.sra SRR ids: ['SRR26075397.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_zl8ldru5 SRR26075397.sra spots: 18204716 blocks: [[1, 910235], [910236, 1820470], [1820471, 2730705], [2730706, 3640940], [3640941, 4551175], [4551176, 5461410], [5461411, 6371645], [6371646, 7281880], [7281881, 8192115], [8192116, 9102350], [9102351, 10012585], [10012586, 10922820], [10922821, 11833055], [11833056, 12743290], [12743291, 13653525], [13653526, 14563760], [14563761, 15473995], [15473996, 16384230], [16384231, 17294465], [17294466, 18204716]] SRR26075397 file size 6717529 SRR26075397 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o SRR26075397 SRR26075397_1.fastq SRR26075397_2.fastq Input file: SRR26075397_1.fastq Paired file: SRR26075397_2.fastq trimmed: SRR26075397-trimmed-pair1.fastq, SRR26075397-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Wed Feb 12 01:02:16 2025 >> started Wed Feb 12 01:02:36 2025 >> done (20.079s) 18204716 read pairs processed; of these: 99 ( 0.00%) short read pairs filtered out after trimming by size control 173873 ( 0.96%) empty read pairs filtered out after trimming by size control 18030744 (99.04%) read pairs available; of these: 3371229 (18.70%) trimmed read pairs available after processing 14659515 (81.30%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 18 10 0.00% 19 9 0.00% 20 8 0.00% 21 12 0.00% 22 17 0.00% 23 13 0.00% 24 12 0.00% 25 15 0.00% 26 20 0.00% 27 21 0.00% 28 27 0.00% 29 26 0.00% 30 21 0.00% 31 27 0.00% 32 40 0.00% 33 35 0.00% 34 30 0.00% 35 27 0.00% 36 28 0.00% 37 37 0.00% 38 39 0.00% 39 44 0.00% 40 46 0.00% 41 41 0.00% 42 70 0.00% 43 58 0.00% 44 55 0.00% 45 64 0.00% 46 91 0.00% 47 67 0.00% 48 89 0.00% 49 111 0.00% 50 116 0.00% 51 116 0.00% 52 151 0.00% 53 129 0.00% 54 133 0.00% 55 219 0.00% 56 196 0.00% 57 225 0.00% 58 251 0.00% 59 295 0.00% 60 304 0.00% 61 424 0.00% 62 449 0.00% 63 465 0.00% 64 575 0.00% 65 577 0.00% 66 729 0.00% 67 836 0.00% 68 858 0.00% 69 1087 0.01% 70 1125 0.01% 71 1299 0.01% 72 1530 0.01% 73 1902 0.01% 74 2082 0.01% 75 2277 0.01% 76 2503 0.01% 77 2964 0.02% 78 3236 0.02% 79 3631 0.02% 80 4213 0.02% 81 4672 0.03% 82 5500 0.03% 83 6360 0.04% 84 7129 0.04% 85 7762 0.04% 86 8465 0.05% 87 9124 0.05% 88 10392 0.06% 89 10690 0.06% 90 11941 0.07% 91 12653 0.07% 92 14037 0.08% 93 15888 0.09% 94 17210 0.10% 95 18220 0.10% 96 19979 0.11% 97 21631 0.12% 98 23016 0.13% 99 23188 0.13% 100 24697 0.14% 101 26167 0.15% 102 27462 0.15% 103 29735 0.16% 104 31824 0.18% 105 33517 0.19% 106 35105 0.19% 107 36608 0.20% 108 37965 0.21% 109 39695 0.22% 110 40817 0.23% 111 42188 0.23% 112 43160 0.24% 113 44130 0.24% 114 46735 0.26% 115 48734 0.27% 116 50122 0.28% 117 51806 0.29% 118 53181 0.29% 119 52614 0.29% 120 54280 0.30% 121 56105 0.31% 122 57134 0.32% 123 58785 0.33% 124 61757 0.34% 125 62643 0.35% 126 64269 0.36% 127 65524 0.36% 128 66395 0.37% 129 68936 0.38% 130 68678 0.38% 131 69583 0.39% 132 70233 0.39% 133 72445 0.40% 134 73751 0.41% 135 74515 0.41% 136 77611 0.43% 137 77516 0.43% 138 77462 0.43% 139 80287 0.45% 140 80085 0.44% 141 82068 0.46% 142 81522 0.45% 143 82123 0.46% 144 83801 0.46% 145 86158 0.48% 146 85276 0.47% 147 86387 0.48% 148 87385 0.48% 149 88486 0.49% 150 89803 0.50% 151 14659515 81.30% 18030744 reads passed initial QC criterion=sequence-density sequence-density=0.36 sequence-density-rank=1 fanout-score=2.20 fanout-score-rank=32 prefix-density=0.38 prefix-fanout=2.1 sequence=TATATATAGCTGCAGATGATACCGCTAATCCTTCTTGGAGTGGATTCTCAAGAAGAACAGCGTCCTGCAGAGCAGCTAGCTAGCGGCTCGCTCTCTATACAAA criterion=fanout-score sequence-density=0.02 sequence-density-rank=34 fanout-score=158.80 fanout-score-rank=1 prefix-density=0.36 prefix-fanout=10.7 sequence=CATCACCATCCCTGATTGATCTTTGATTCACAACAAGACCAACCAACCGTACAATGTTTACATAACTTGGGATAATCTCACAAGAAAGAAAGATCAATACAAAACTAGTCAGCTCAGGATGTTCCAGGCACGGTAGAACCGTAGAACCATAACAACAAGAGACATATTGCAGATGAGTACTGAAAAACAAAACACAGTACGTATTTACATGGGCAACCTTGGTTGAAGGCAACCTCATCAACGATGCTCGCTCTTCGTCTCTCCACTGTACATCCAGTCATAGACAGTGGGTGTGTTAGGCTGTGGCTTGTCAAAAACATGAGCACCAATATTCTTAGTAGCAAGGTTGCTACCAGGGTGGAACACGCTCCTCCAAACATTGCTACGCGCCGACACTGGGGTTGTAGGGGTCACTGGTGTCGTCGGTGTCCCTGGAGTTCCTGGCATAGTCATGGACCTCTGAAACTTATTAACAGGACTGCTCCCCTCTCCGACGTCAATATCTTTGATG criterion=sequence-density sequence-density=0.48 sequence-density-rank=1 fanout-score=2.91 fanout-score-rank=34 prefix-density=0.73 prefix-fanout=1.9 sequence=ATATATATACACGAAAACCATAAATAAAATGGCTCGCTCTCTCCCAAACGCTAAGCTTCTTGTCGCTTCTCTTGCTGATGGTCTTTCCCTCTCTGTTTTCCGGAGAGGTTACGCGGCTGCTGCACCGATCAGTGCTGCTGTAACAGCGAGCTTTGGTAGGGGTGGGTCAAGGACCAGTGCTATGACAGGGAAAATGGAAGATGGGGCGGTGGCCAAAGAAGATTCTGAGGCCTACTCTGCATGGGCTCCTGATCCAGTTACTGGGTATTACAGGCCTGCTAATTATGTAGCAGAGATCGATCCAGCTGAACTTAGGGAAATGGTGTTGAGCCATAGGGTTAGGCCACAGTAGAGGTTGCAGGATATGGTTCTGTTACTAGATGGTGGTGGCTATGGGTTTGGTCGCGTCACGCTCT criterion=fanout-score sequence-density=0.11 sequence-density-rank=21 fanout-score=361.15 fanout-score-rank=1 prefix-density=1.23 prefix-fanout=31.4 sequence=AAGAAGAAGAAA SRR26075397 testing PE reads STAR mapping to Ensembl genome Started job on | Feb 12 01:03:36 Started mapping on | Feb 12 01:03:37 Finished on | Feb 12 01:06:36 Mapping speed, Million of reads per hour | 362.63 Number of input reads | 18030744 Average input read length | 284 UNIQUE READS: Uniquely mapped reads number | 13575199 Uniquely mapped reads % | 75.29% Average mapped length | 285.34 Number of splices: Total | 12989453 Number of splices: Annotated (sjdb) | 12651815 Number of splices: GT/AG | 12748247 Number of splices: GC/AG | 181752 Number of splices: AT/AC | 16899 Number of splices: Non-canonical | 42555 Mismatch rate per base, % | 0.46% Deletion rate per base | 0.04% Deletion average length | 3.55 Insertion rate per base | 0.02% Insertion average length | 2.56 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 370160 % of reads mapped to multiple loci | 2.05% Number of reads mapped to too many loci | 100579 % of reads mapped to too many loci | 0.56% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 21.53% % of reads unmapped: other | 0.57% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 4085387 4085387 4085387 N_multimapping 370160 370160 370160 N_noFeature 292010 13425928 375600 N_ambiguous 269504 2414 202192 UnstrandedReadsAssigned:13013685 PositiveStrandReadsAssigned:146857 NegativeStrandReadsAssigned:12997407 Dataset is classified negative stranded MeadianReadLen=151 20thPercentileLength=151 echo kmer=147 SRR26075397 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,400 [index] number of k-mers: 62,057,036 [index] number of equivalence classes: 130,681 [quant] running in paired-end mode [quant] will process pair 1: SRR26075397-trimmed-pair1.fastq SRR26075397-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 18,030,744 reads, 15,670,273 reads pseudoaligned [quant] estimated average fragment length: 198.735 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,163 rounds 52401 SRR26075397.ke.tsv 34699 SRR26075397.se.tsv 87100 total ==> SRR26075397.ke.tsv <== target_id length eff_length est_counts tpm Potri.005G200100.1.v4.1 2018 1820.26 2056 59.6998 Potri.005G024800.1.v4.1 1035 837.265 4335 273.659 Potri.004G059700.1.v4.1 961 763.27 7 0.484735 Potri.007G009000.2.v4.1 1416 1218.26 0 0 Potri.003G141000.2.v4.1 2943 2745.26 1050 20.2157 Potri.016G087400.1.v4.1 270 99.8868 1818.51 962.258 Potri.015G069301.1.v4.1 564 367.689 0 0 Potri.010G195200.1.v4.1 1773 1575.26 107 3.59016 Potri.012G127500.1.v4.1 977 779.27 2546 172.685 ==> SRR26075397.se.tsv <== Potri.001G166300.v4.1 0 Potri.001G448400.v4.1 5 Potri.001G233950.v4.1 0 Potri.001G122700.v4.1 115 Potri.001G212900.v4.1 0 Potri.001G182400.v4.1 0 Potri.001G256600.v4.1 0 Potri.001G040500.v4.1 0 Potri.001G416900.v4.1 0 Potri.001G452600.v4.1 285 SRR26075397 completed mapping pipeline successfully